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[PMID]:28895509
[Au] Autor:Yavari CA; Ramírez AS; Nicholas RAJ; Radford AD; Darby AC; Bradbury JM
[Ad] Endereço:1​University of Liverpool, Institute of Infection and Global Health, Leahurst Campus, Neston, CH64 7TE, UK.
[Ti] Título:Mycoplasma tullyi sp. nov., isolated from penguins of the genus Spheniscus.
[So] Source:Int J Syst Evol Microbiol;67(10):3692-3698, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A mycoplasma isolated from the liver of a dead Humboldt penguin (Spheniscus humboldti) and designated strain 56A97 , was investigated to determine its taxonomic status. Complete 16S rRNA gene sequence analysis indicated that the organism was most closely related to Mycoplasma gallisepticum and Mycoplasma imitans(99.7 and 99.9 % similarity, respectively). The average DNA-DNA hybridization values between strain 56A97 and M. gallisepticum and M. imitans were 39.5 and 30 %, respectively and the Genome to Genome Distance Calculator gave results of 29.10 and 23.50 %, respectively. The 16S-23S rRNA intergenic spacer was 72-73 % similar to M. gallisepticum strains and 52.2 % to M. imitans. A partial sequence of rpoB was 91.1-92 % similar to M. gallisepticum strains and 84.7 % to M. imitans. Colonies possessed a typical fried-egg appearance and electron micrographs revealed the lack of a cell wall and a nearly spherical morphology, with an electron-dense tip-like structure on some flask-shaped cells. The isolate required sterol for growth, fermented glucose, adsorbed and haemolysed erythrocytes, but did not hydrolyse arginine or urea. The strain was compared serologically against 110 previously described Mycoplasma reference strains, showing that, except for M. gallisepticum, strain 56A97 is not related to any of the previously described species, although weak cross-reactions were evident. Genomic information, serological reactions and phenotypic properties demonstrate that this organism represents a novel species of the genus Mycoplasma, for which the name Mycoplasma tullyi sp. nov. is proposed; the type strain is 56A97 (ATCC BAA-1432 , DSM 21909 , NCTC 11747 ).
[Mh] Termos MeSH primário: Fígado/microbiologia
Mycoplasma/classificação
Filogenia
Spheniscidae/microbiologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
DNA Bacteriano/genética
Mycoplasma/genética
Mycoplasma/isolamento & purificação
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002052


  2 / 8877 MEDLINE  
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[PMID]:28859111
[Au] Autor:Woo S; Yang SH; Chen HJ; Tseng YF; Hwang SJ; De Palmas S; Denis V; Imahara Y; Iwase F; Yum S; Tang SL
[Ad] Endereço:Korea Institute of Ocean Science & Technology, Geoje, Republic of Korea.
[Ti] Título:Geographical variations in bacterial communities associated with soft coral Scleronephthya gracillimum.
[So] Source:PLoS One;12(8):e0183663, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Environmental impacts can alter relationships between a coral and its symbiotic microbial community. Furthermore, changes in the microbial community associated with increased seawater temperatures can cause opportunistic infections, coral disease and death. Interactions between soft corals and their associated microbes are not well understood. The species Scleronephthya gracillimum is distributed in tropical to temperate zones in coral assemblages along the Kuroshio Current region. In this study we collected S. gracillimum from various sites at different latitudes, and compared composition of their bacterial communities using Next Generation Sequencing. Coral samples from six geographically distinct areas (two sites each in Taiwan, Japan, and Korea) had considerable variation in their associated bacterial communities and diversity. Endozoicimonaceae was the dominant group in corals from Korea and Japan, whereas Mycoplasma was dominant in corals from Taiwan corals. Interestingly, the latter corals had lower relative abundance of Endozoicimonaceae, but greater diversity. These biogeographic differences in bacterial composition may have been due to varying environmental conditions among study locations, or because of host responses to prevailing environmental conditions. This study provided a baseline for future studies of soft coral microbiomes, and assessment of functions of host metabolites and soft coral holobionts.
[Mh] Termos MeSH primário: Antozoários/genética
Antozoários/microbiologia
Mycoplasma/genética
Simbiose/genética
[Mh] Termos MeSH secundário: Animais
Antozoários/crescimento & desenvolvimento
Bactérias/classificação
Bactérias/genética
Monitoramento Ambiental
Geografia
Sequenciamento de Nucleotídeos em Larga Escala
Japão
Mycoplasma/isolamento & purificação
RNA Ribossômico 16S/genética
República da Coreia
Água do Mar/microbiologia
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183663


  3 / 8877 MEDLINE  
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[PMID]:28821624
[Au] Autor:Hsieh J; Yalcindag A; Coghlin DT
[Ad] Endereço:Hasbro Children's Hospital, Warren Alpert Medical School at Brown University, Providence, Rhode Island daniel.coghlin@brown.edu.
[Ti] Título:A Sweet Case of .
[So] Source:Pediatrics;140(3), 2017 Sep.
[Is] ISSN:1098-4275
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sweet syndrome, also known as acute febrile neutrophilic dermatosis, is an uncommon inflammatory disorder marked by fever and swelling of the skin that can be very painful. It is especially rare in the pediatric population. Infection is a well-known trigger for Sweet syndrome, but this entity has, to our knowledge, never been described after infection. Herein, we describe the first pediatric case of febrile neutrophilic dermatosis associated with infection.
[Mh] Termos MeSH primário: Infecções por Mycoplasma/complicações
Mycoplasma
Síndrome de Sweet/complicações
[Mh] Termos MeSH secundário: Criança
Seres Humanos
Masculino
Pele
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170820
[St] Status:MEDLINE


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[PMID]:28652181
[Au] Autor:Zhao Y; Wang Z; Hou Y; Zhang K; Peng X
[Ad] Endereço:Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction (Huazhong Agricultural University), Ministry of Education, College of Animal Science and Technology & College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
[Ti] Título:gga-miR-99a targets SMARCA5 to regulate Mycoplasma gallisepticum (HS strain) infection by depressing cell proliferation in chicken.
[So] Source:Gene;627:239-247, 2017 Sep 05.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mycoplasma gallisepticum (MG), one of the primary etiological agents of poultry chronic respiratory disease, has caused significant economic losses worldwide, and increasing evidence has recently indicated that miRNAs are involved in its microbial pathogenesis. gga-miR-99a, a member of the miR-99 family, plays an essential role in a variety of diseases. Through miRNA Solexa sequencing, we previously found that gga-miR-99a is significantly down-regulated in the lungs of MG-infected chicken embryos. In this study, we further verified that the expression of gga-miR-99 was significantly down-regulated in both MG-infected lungs and a chicken embryonic fibroblast cell line (DF-1) by qPCR. Moreover, we predicted and validated SMARCA5 as its target gene through a luciferase reporter assay, qPCR, and western blot analysis. The over-expression of gga-miR-99a significantly depressed SMARCA5 expression, whereas a gga-miR-99a inhibitor enhanced the expression of SMARCA5. Inversely, SMARCA5 was significantly up-regulated and gga-miR-99a was obviously down-regulated in MG-HS-infected chicken embryonic lungs and DF-1 cells. At 72h post-transfection, the over-expression of gga-miR-99a significantly repressed the proliferation of DF-1 cells by inhibiting the transition from the G1 phase to the S and G2 phases. This study reveals that gga-miR-99a plays a key role in MG infection through the regulation of SMARCA5 expression and provides new insights regarding the mechanisms of MG pathogenesis.
[Mh] Termos MeSH primário: Doenças das Aves/genética
Proliferação Celular
Proteínas Cromossômicas não Histona/genética
MicroRNAs/genética
Infecções por Mycoplasma/genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Embrião de Galinha
Galinhas
Proteínas Cromossômicas não Histona/metabolismo
Fibroblastos/metabolismo
Fibroblastos/microbiologia
Fibroblastos/fisiologia
Mycoplasma/patogenicidade
Infecções por Mycoplasma/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Chromosomal Proteins, Non-Histone); 0 (MicroRNAs)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE


  5 / 8877 MEDLINE  
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[PMID]:28549435
[Au] Autor:Wang X; Cui Y; Zhang Y; Shi K; Yan Y; Jian F; Zhang L; Wang R; Ning C
[Ad] Endereço:College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
[Ti] Título:Molecular characterization of hemotropic mycoplasmas (Mycoplasma ovis and 'Candidatus Mycoplasma haemovis') in sheep and goats in China.
[So] Source:BMC Vet Res;13(1):142, 2017 May 26.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hemotropic mycoplasmas (hemoplasmas) are emerging zoonotic pathogens with a worldwide distribution that can cause mild to severe hemolytic anemia, icterus, ill-thrift, infertility, and poor weight gain. However, understanding of the molecular epidemiology of hemoplasmas (Mycoplasma ovis and 'Candidatus Mycoplasma haemovis') is limited in sheep and goats, and the hemoplasma strain/species/variant 'Candidatus M. haemovis' was poorly studied throughout the world and had never been detected in China until now. Thus, the aim of the present study was to determine the molecular prevalence of hemoplasmas, including M. ovis and 'Candidatus M. haemovis' in sheep and goats from seven provinces and one autonomous region of China. METHODS: A total of 1364 blood samples were collected from sheep and goats in seven provinces and one autonomous region of China. All blood samples were tested for hemoplasmas (M. ovis and 'Candidatus M. haemovis') by nested PCR amplification based on 16S rRNA gene. Positive specimens underwent nucleotide sequencing and phylogenetic analysis. RESULTS: Overall, 610 specimens (44.7%, 610/1364) were shown to be hemoplasmas (M. ovis and 'Candidatus M. haemovis') -positive by nested PCR amplification based on 16S rRNA gene. The prevalence in goats was 44.1% (379/860), and 45.8% (231/504) in sheep, while that in grazing small ruminants was 54.4% (396/728) and 33.6% (214/636) in house feeding small ruminants. Sequencing of the nearly complete 16S rRNA gene was successful for the 103 randomly selected positive specimens from different farms in different sampling sites of China. Among them, analysis of the 16S rRNA gene sequences identified M. ovis (n = 56) and 'Candidatus M. haemovis' (n = 47). Two (KU983740 and KU983746) of the four novel genotypes obtained in this study were closely related to M. ovis, while the other two genotypes (KU983748 and KU983749) had high identity with 'Candidatus M. haemovis'. Remarkably, the genotype (KU983740) of M. ovis in sheep and goats in this study fell in a clade with two human hemoplasmas from USA (KF313922 and GU230144) and shared 99.8%-99.9% with them. CONCLUSIONS: In this study, 'Candidatus M. haemovis' was first detected in Chinese sheep and goats and hemoplasmas (M. ovis and 'Candidatus M. haemovis') are highly prevalent, and widely distributed in China.
[Mh] Termos MeSH primário: Doenças das Cabras/microbiologia
Infecções por Mycoplasma/veterinária
Mycoplasma/isolamento & purificação
Doenças dos Ovinos/microbiologia
[Mh] Termos MeSH secundário: Animais
China/epidemiologia
Feminino
Doenças das Cabras/epidemiologia
Cabras
Masculino
Tipagem Molecular
Mycoplasma/classificação
Mycoplasma/genética
Infecções por Mycoplasma/epidemiologia
Infecções por Mycoplasma/genética
Filogenia
Prevalência
RNA Bacteriano
RNA Ribossômico 16S
Ovinos
Doenças dos Ovinos/epidemiologia
Zoonoses/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170528
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-017-1062-z


  6 / 8877 MEDLINE  
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[PMID]:28541806
[Au] Autor:Barie PS
[Ad] Endereço:Departments of Surgery and Medicine, Weill Cornell Medicine , New York, New York.
[Ti] Título:Atypical Wound Pathogens.
[So] Source:Surg Infect (Larchmt);18(4):455-460, 2017 May/Jun.
[Is] ISSN:1557-8674
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Atypical wound pathogens may be so described because they are uncommon pathogens of soft tissue among human beings, or because they may be fastidious and difficult to recover/isolate in the laboratory. METHODS: A review of pertinent English-language literature was performed. RESULTS: These wound pathogens are a diverse lot, including aerobic and anaerobic gram-positive and gram-negative bacilli, non-tuberculous mycobacteria, and bacteria that cannot be characterized conventionally because they lack a cell wall (the Mycoplasmataceae). They are diverse with respect to their virulence, but many are opportunistic pathogens. CONCLUSIONS: Among these atypical pathogens, clinical reports are most common of wound infections caused by Mycoplasma/Ureaplasma (sometimes as co-infecting agents), and the so-called rapidly growing non-tuberculous mycobacteria (Runyon Type IV; e.g., M. chelonae).
[Mh] Termos MeSH primário: Infecção da Ferida Cirúrgica/microbiologia
[Mh] Termos MeSH secundário: Bacteroides
Seres Humanos
Mycoplasma
Micobactérias não Tuberculosas
Ureaplasma
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1089/sur.2017.100


  7 / 8877 MEDLINE  
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[PMID]:28502279
[Au] Autor:Ikeda P; Seki MC; Carrasco AOT; Rudiak LV; Miranda JMD; Gonçalves SMM; Hoppe EGL; Albuquerque ACA; Teixeira MMG; Passos CE; Werther K; Machado RZ; André MR
[Ad] Endereço:Faculdade de Ciências Agrárias e Veterinárias,Universidade Estadual Paulista (Unesp),Jaboticabal, SP,Brazil.
[Ti] Título:Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil.
[So] Source:Epidemiol Infect;145(10):2038-2052, 2017 07.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The order Chiroptera is considered the second largest group of mammals in the world, hosting important zoonotic virus and bacteria. Bartonella and hemotropic mycoplasmas are bacteria that parasite different mammals' species, including humans, causing different clinical manifestations. The present work aimed investigating the occurrence and assessing the phylogenetic positioning of Bartonella spp. and Mycoplasma spp. in neotropical bats sampled from Brazil. Between December 2015 and April 2016, 325 blood and/or tissues samples were collected from 162 bats comprising 19 different species sampled in five states of Brazil. Out of 322 bat samples collected, while 17 (5·28%) were positive to quantitative PCR for Bartonella spp. based on nuoG gene, 45 samples (13·97%) were positive to cPCR assays for hemoplasmas based on 16S rRNA gene. While seven sequences were obtained for Bartonella (nuoG) (n = 3), gltA (n = 2), rpoB (n = 1), ftsZ (n = 1), five 16S rRNA sequences were obtained for hemoplasmas. In the phylogenetic analysis, the Bartonella sequences clustered with Bartonella genotypes detected in bats sampled in Latin America countries. All five hemoplasmas sequences clustered together as a monophyletic group by Maximum Likelihood and Bayesian Inference analyses. The present work showed the first evidence of circulation of Bartonella spp. and hemoplasmas among bats in Brazil.
[Mh] Termos MeSH primário: Infecções por Bartonella/veterinária
Bartonella/genética
Quirópteros
Infecções por Mycoplasma/veterinária
Mycoplasma/genética
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Bartonella/isolamento & purificação
Infecções por Bartonella/epidemiologia
Infecções por Bartonella/microbiologia
Brasil/epidemiologia
DNA Bacteriano/genética
Mycoplasma/isolamento & purificação
Infecções por Mycoplasma/epidemiologia
Infecções por Mycoplasma/microbiologia
Filogenia
Análise de Sequência de DNA/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1017/S0950268817000966


  8 / 8877 MEDLINE  
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[PMID]:28390431
[Au] Autor:Cornelissen JB; de Bree FM; van der Wal FJ; Kooi EA; Koene MG; Bossers A; Smid B; Antonis AF; Wisselink HJ
[Ad] Endereço:Department of Infection Biology, Central Veterinary Institute of Wageningen UR, P.O. Box 65, 8200, AB, Lelystad, The Netherlands. jan.cornelissen@wur.nl.
[Ti] Título:Mycoplasma detection by triplex real-time PCR in bronchoalveolar lavage fluid from bovine respiratory disease complex cases.
[So] Source:BMC Vet Res;13(1):97, 2017 Apr 08.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In this study we evaluated the RespoCheck Mycoplasma triplex real-time PCR for the detection in bronchoalveolar lavage fluid (BALF) of Mycoplasma (M.) dispar, M. bovis and M. bovirhinis, all three associated with bovine respiratory disease (BRD). Primers and probes of the RespoCheck Mycoplasma triplex real-time PCR are based on the V3/V4 region of the 16S rRNA gene of the three Mycoplasma species. RESULTS: The analytical sensitivity of the RespoCheck triplex real-time PCR was, as determined by spiking experiments of the Mycoplasma strains in Phosphate Buffered Saline, 300 colony forming units (cfu)/mL for M. dispar, and 30 cfu/mL for M. bovis or M. bovirhinis. The analytical sensitivity of the RespoCheck Mycoplasma triplex real-time PCRwas, as determined on purified DNA, 10 fg DNA per assay for M. dispar and 100 fg fo rM. bovis and M. bovirhinis. The analytical specificity of the RespoCheck Mycoplasma triplex real-time PCR was, as determined by testing Mycoplasmas strains (n = 17) and other bacterial strains (n = 107), 100, 98.2 and 99.1% for M. bovis, M. dispar and M. bovirhinis respectively. The RespoCheck Mycoplasma triplex real-time PCR was compared with the PCR/DGGE analysis for M. bovis, M. dispar and M. bovirhinis respectively by testing 44 BALF samples from calves. CONCLUSION: In conclusion, the RespoCheck PCR assay can be a valuable tool for timely and accurate detection of three Mycoplasma species associated with in bovine respiratory disease.
[Mh] Termos MeSH primário: Complexo Respiratório Bovino/diagnóstico
Infecções por Mycoplasma/veterinária
Mycoplasma/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Complexo Respiratório Bovino/microbiologia
Líquido da Lavagem Broncoalveolar/microbiologia
Bovinos
Mycoplasma/genética
Infecções por Mycoplasma/diagnóstico
RNA Ribossômico 16S/genética
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Sensibilidade e Especificidade
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170410
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-017-1023-6


  9 / 8877 MEDLINE  
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[PMID]:28285864
[Au] Autor:Ishag HZ; Xiong Q; Liu M; Feng Z; Shao G
[Ad] Endereço:Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Research Center for Engineering and Technology of Veterinary Bio-products, Nanjing 210014, China; College of Veterinary Sc
[Ti] Título:E. coli recA gene improves gene targeted homologous recombination in Mycoplasma hyorhinis.
[So] Source:J Microbiol Methods;136:49-56, 2017 May.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mycoplasma hyorhinis is an opportunistic pathogen of pigs. Recently, it has been shown to transform cell cultures, increasing the attention of the researchers. Studies on the pathogenesis require specific genetic tool that is not yet available for the pathogen. To address this limitation, we constructed two suicide plasmids pGEMT-tetM/LR and pGEMT-recA-tetM/LR having a tetracycline resistance marker flanked by two hemolysin gene arms. The latter plasmid encodes an E. coli recA, a gene involved in DNA recombination, repair and maintenance of DNA. Using inactivation of the hemolysin gene, which results in a detectable and measurable phenotype, we found that each plasmid can disrupt the hemolysin gene of M. hyorhinis through a double cross-over homologous recombination. However, inclusion of the E. coli recA gene in the construct resulted in 9-fold increase in the frequency of hemolysin gene mutants among the screened tetracycline resistance colonies. The resultant hemolysin mutant strain lacks the ability to lyse mouse bed blood cells (RBC) when tested in vitro (p<0.001). The host-plasmid system described in this study, has applications for the genetic manipulation of this pathogen and potentially other mycoplasmas.
[Mh] Termos MeSH primário: Escherichia coli/genética
Marcação de Genes/métodos
Recombinação Homóloga
Mycoplasma hyorhinis/genética
Recombinases Rec A/genética
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
DNA Bacteriano
Genes Bacterianos
Vetores Genéticos
Proteínas Hemolisinas/genética
Camundongos
Mutação
Mycoplasma/genética
Infecções por Mycoplasma/diagnóstico
Mycoplasma hyorhinis/patogenicidade
Fenótipo
Plasmídeos
Regiões Promotoras Genéticas
Proteínas Recombinantes/genética
Recombinação Genética
Reparo de DNA por Recombinação
Suínos
Resistência a Tetraciclina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Hemolysin Proteins); 0 (Recombinant Proteins); EC 2.7.7.- (Rec A Recombinases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE


  10 / 8877 MEDLINE  
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[PMID]:28285597
[Au] Autor:Attipa C; Papasouliotis K; Solano-Gallego L; Baneth G; Nachum-Biala Y; Sarvani E; Knowles TG; Mengi S; Morris D; Helps C; Tasker S
[Ad] Endereço:Molecular Diagnostic Unit, Diagnostic Laboratories, Langford Vets and School of Veterinary Sciences, University of Bristol, Langford, UK. attipacy@gmail.com.
[Ti] Título:Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus.
[So] Source:Parasit Vectors;10(1):130, 2017 Mar 13.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Feline infectious agent studies are lacking in Cyprus. The aims of this study were to determine the prevalence and risk factors for various feline infectious agents, including feline vector-borne pathogens (FVBP), in cats from Cyprus. METHODS: A cross-sectional, descriptive, multicentre study was performed on 174 feline samples [138 owned and 36 shelter-feral, including both healthy (43) and non-healthy (131), cats] from private veterinary clinics from all six districts of Cyprus. Real-time quantitative polymerase chain reaction (qPCR) assays were used to detect Mycoplasma haemofelis (Mhf), "Candidatus Mycoplasma haemominutum" (CMhm) and "Candidatus Mycoplasma turicensis" (CMt). The population was tested for four FVBP including Bartonella henselae and Leishmania spp. using qPCR, while conventional PCR assays were used to detect Ehrlichia/Anaplasma spp. and Hepatozoon spp. Serological assays were performed to detect Leishmania infantum antibodies, feline leukaemia virus (FeLV) antigen and feline immunodeficiency virus (FIV) antibodies. Statistical analysis was performed to test associations and possible risk factors between variables and infectious agents. RESULTS: Ninety-six (55.2%) of the 174 cats were PCR-positive for at least one infectious agent. Forty-six cats (26.4%) were haemoplasma positive, including 13 (7.5%) for Mhf, 36 (20.7%) for CMhm and 12 (6.9%) for CMt. Sixty-six cats (37.9%) were positive for Hepatozoon spp., while 19 (10.9%) were positive for B. henselae, four (2.3%) for Leishmania spp. and one (0.6%) for Ehrlichia/Anaplasma spp. Sequencing revealed the presence of Hepatozoon felis, L. infantum and Anaplasma platys. Of the 164 cats that underwent retroviral serology, 10 (6.1%) were FeLV-positive and 31 (18.9%) were FIV-positive, while L. infantum serology was positive in 7 (4.4%) of the 160 cats tested. Multivariable logistic regression revealed significant associations for various infectious agents including L. infantum with each of Hepatozoon spp. and CMt infection. CONCLUSIONS: A high prevalence of infectious agents was found in cats from Cyprus with Mhf, CMhm, CMt, L. infantum, B. henselae, H. felis, A. platys, FeLV and FIV infections reported for the first time. The significant associations between different pathogens provide a better understanding of similarities in the epidemiology of these pathogens and interactions between them.
[Mh] Termos MeSH primário: Infecções Bacterianas/veterinária
Doenças do Gato/epidemiologia
Infecções por Mycoplasma/veterinária
Infecções Protozoárias em Animais/epidemiologia
Infecções por Retroviridae/veterinária
Doenças Transmitidas por Carrapatos/veterinária
[Mh] Termos MeSH secundário: Anaplasma/genética
Anaplasma/isolamento & purificação
Anaplasmose/epidemiologia
Anaplasmose/microbiologia
Animais
Infecções Bacterianas/epidemiologia
Infecções Bacterianas/microbiologia
Doenças do Gato/microbiologia
Doenças do Gato/parasitologia
Doenças do Gato/virologia
Gatos
Coccidiose/epidemiologia
Coccidiose/veterinária
Estudos Transversais
Chipre/epidemiologia
Ehrlichia/genética
Ehrlichia/isolamento & purificação
Ehrlichiose/epidemiologia
Ehrlichiose/microbiologia
Ehrlichiose/veterinária
Análise Fatorial
Feminino
Leishmania infantum/genética
Leishmania infantum/isolamento & purificação
Leishmaniose Visceral/epidemiologia
Leishmaniose Visceral/parasitologia
Leishmaniose Visceral/veterinária
Vírus da Leucemia Felina/genética
Vírus da Leucemia Felina/isolamento & purificação
Masculino
Mycoplasma/genética
Mycoplasma/isolamento & purificação
Infecções por Mycoplasma/epidemiologia
Infecções por Mycoplasma/microbiologia
Prevalência
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Análise de Regressão
Infecções por Retroviridae/epidemiologia
Infecções por Retroviridae/virologia
Medição de Risco
Fatores de Risco
Doenças Transmitidas por Carrapatos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2063-2



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