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[PMID]:28845949
[Au] Autor:Khryanin AA; Reshetnikov OV
[Ad] Endereço:Novosibirsk State Medical University of Minzdrav of Russia, Novosibirsk, Russia.
[Ti] Título:[The role of mycoplasma infection (M. hominis and U. urealyticum) in inflammatory diseases of the genitourinary system the debate continues].
[So] Source:Urologiia;(3):112-119, 2017 Jul.
[Is] ISSN:1728-2985
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The article presents current views on urogenital diseases caused by M. hominis and U. urealyticum. Particular attention is paid to concurrent (co-occurring) urogenital infections. The review reports the data from epidemiological studies and outlines recent Russian and international guidelines and consensuses on managing patients with urogenital inflammatory diseases. The importance of adequate diagnosis and rational therapy of urogenital infections is discussed.
[Mh] Termos MeSH primário: Doenças Urogenitais Femininas/microbiologia
Doenças Urogenitais Masculinas/microbiologia
Infecções por Mycoplasma/complicações
Mycoplasma hominis/patogenicidade
Infecções por Ureaplasma/complicações
Ureaplasma urealyticum/patogenicidade
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Masculino
Mycoplasma hominis/isolamento & purificação
Ureaplasma urealyticum/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE


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[PMID]:28806998
[Au] Autor:Kotaskova I; Nemec P; Vanerkova M; Malisova B; Tejkalova R; Orban M; Zampachova V; Freiberger T
[Ad] Endereço:Molecular and Genetics Laboratory, Centre for Cardiovascular Surgery and Transplantation, Brno, Czech Republic.
[Ti] Título:First report of Sneathia sanguinegens together with Mycoplasma hominis in postpartum prosthetic valve infective endocarditis: a case report.
[So] Source:BMC Infect Dis;17(1):563, 2017 Aug 14.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The presence of more than one bacterial agent is relatively rare in infective endocarditis, although more common in prosthetic cases. Molecular diagnosis from a removed heart tissue is considered a quick and effective way to diagnose fastidious or intracellular agents. CASE PRESENTATION: Here we describe the case of postpartum polymicrobial prosthetic valve endocarditis in a young woman. Sneathia sanguinegens and Mycoplasma hominis were simultaneously detected from the heart valve sample using broad range 16S rRNA polymerase chain reaction (PCR) followed by sequencing while culture remained negative. Results were confirmed by independent PCR combined with denaturing gradient gel electrophoresis. Before the final agent identification, the highly non-compliant patient left from the hospital against medical advice on empirical intravenous treatment with aminopenicillins, clavulanate and gentamicin switched to oral amoxycillin and clavulanate. Four months after surgery, no signs of inflammation were present despite new regurgitation and valve leaflet flail was detected. However, after another 5 months the patient died from sepsis and recurrent infective endocarditis of unclarified etiology. CONCLUSIONS: Mycoplasma hominis is a rare causative agent of infective endocarditis. To the best of our knowledge, presented case is the first report of Sneathia sanguinegens detected in this condition. Molecular techniques were shown to be useful even in polymicrobial infective endocarditis samples.
[Mh] Termos MeSH primário: Endocardite Bacteriana/microbiologia
Infecções por Fusobacteriaceae/microbiologia
Leptotrichia/patogenicidade
Mycoplasma hominis/patogenicidade
Infecções Relacionadas à Prótese/microbiologia
[Mh] Termos MeSH secundário: Adulto
Amoxicilina/uso terapêutico
Antibacterianos/uso terapêutico
Endocardite Bacteriana/tratamento farmacológico
Feminino
Próteses Valvulares Cardíacas
Seres Humanos
Leptotrichia/genética
Leptotrichia/isolamento & purificação
Masculino
Infecções por Mycoplasma/tratamento farmacológico
Infecções por Mycoplasma/microbiologia
Mycoplasma hominis/genética
Período Pós-Parto
Gravidez
Infecções Relacionadas à Prótese/tratamento farmacológico
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (RNA, Ribosomal, 16S); 804826J2HU (Amoxicillin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2654-8


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[PMID]:28753664
[Au] Autor:Henrich B; Kretzmer F; Deenen R; Köhrer K
[Ad] Endereço:Institute of Medical Microbiology and Hospital Hygiene, Medical Faculty of Heinrich-Heine-University Duesseldorf, Duesseldorf, Germany.
[Ti] Título:Validation of a novel Mho microarray for a comprehensive characterisation of the Mycoplasma hominis action in HeLa cell infection.
[So] Source:PLoS One;12(7):e0181383, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycoplasma hominis is the second smallest facultative pathogen of the human urogenital tract. With less than 600 protein-encoding genes, it represents an ideal model organism for the study of host-pathogen interactions. For a comprehensive characterisation of the M. hominis action in infection a customized Mho microarray, which was based on two genome sequences (PG21 and LBD-4), was designed to analyze the dynamics of the mycoplasma transcriptome during infection and validated for M. hominis strain FBG. RNA preparation was evaluated and adapted to ensure the highest recovery of mycoplasmal mRNAs from in vitro HeLa cell infection assays. Following cRNA hybridization, the read-out strategy of the hybridization results was optimized and confirmed by RT-PCR. A statistically robust infection assay with M. hominis strain FBG enabled the identification of differentially regulated key effector molecules such as critical cytoadhesins (4 h post infection (pI)), invasins (48 h pI) and proteins associated with establishing chronic infection of the host (336 h pI). Of the 294 differentially regulated genes (>2-fold) 128 (43.5%) encoded hypothetical proteins, including lipoproteins that seem to play a central role as virulence factors at each stage of infection: P75 as a novel cytoadhesin candidate, which is also differentially upregulated in chronic infection; the MHO_2100 protein, a postulated invasin and the MHO_730-protein, a novel ecto-nuclease and domain of an ABC transporter, the function of which in chronic infection has still to be elucidated. Implementation of the M. hominis microarray strategy led to a comprehensive identification of to date unknown candidates for virulence factors at relevant stages of host cell infection.
[Mh] Termos MeSH primário: Mycoplasma hominis/genética
Mycoplasma hominis/patogenicidade
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Genoma Bacteriano/genética
Células HeLa
Seres Humanos
RNA Mensageiro/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (RNA, Messenger)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170729
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181383


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[PMID]:28559291
[Au] Autor:Goret J; Béven L; Faustin B; Contin-Bordes C; Le Roy C; Claverol S; Renaudin H; Bébéar C; Pereyre S
[Ad] Endereço:Univ. Bordeaux, USC EA 3671 Mycoplasmal and chlamydial infections in humans, Bordeaux, France.
[Ti] Título:Interaction of Mycoplasma hominis PG21 with Human Dendritic Cells: Interleukin-23-Inducing Mycoplasmal Lipoproteins and Inflammasome Activation of the Cell.
[So] Source:J Bacteriol;199(15), 2017 Aug 01.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:lacks a cell wall, and lipoproteins anchored to the extracellular side of the plasma membrane are in direct contact with the host components. A Triton X-114 extract of enriched with lipoproteins was shown to stimulate the production of interleukin-23 (IL-23) by human dendritic cells (hDCs). The inflammasome activation of the host cell has never been reported upon infection. We studied here the interaction between PG21 and hDCs by analyzing both the inflammation-inducing mycoplasmal lipoproteins and the inflammasome activation of the host cell. IL-23-inducing lipoproteins were determined using a sequential extraction strategy with two nondenaturing detergents, Sarkosyl and Triton X-114, followed by SDS-PAGE separation and mass spectrometry identification. The activation of the hDC inflammasome was assessed using PCR array and enzyme-linked immunosorbent assay (ELISA). We defined a list of 24 lipoproteins that could induce the secretion of IL-23 by hDCs, 5 with a molecular mass between 20 and 35 kDa and 19 with a molecular mass between 40 and 100 kDa. Among them, lipoprotein MHO_4720 was identified as potentially bioactive, and a synthetic lipopeptide corresponding to the N-terminal part of the lipoprotein was subsequently shown to induce IL-23 release by hDCs. Regarding the hDC innate immune response, inflammasome activation with caspase-dependent production of IL-1ß was observed. After 24 h of coincubation of hDCs with s, downregulation of the NLRP3-encoding gene and of the adaptor PYCARD-encoding gene was noticed. Overall, this study provides insight into both protagonists of the interaction of and hDCs. is a human urogenital pathogen involved in gynecologic and opportunistic infections. lacks a cell wall, and its membrane contains many lipoproteins that are anchored to the extracellular side of the plasma membrane. In the present study, we focused on the interaction between and human dendritic cells and examined both sides of the interaction, the mycoplasmal lipoproteins involved in the activation of the host cell and the immune response of the cell. On the mycoplasmal side, we showed for the first time that lipoproteins with high molecular mass were potentially bioactive. On the cell side, we reported an activation of the inflammasome, which is involved in the innate immune response.
[Mh] Termos MeSH primário: Células Dendríticas/imunologia
Interações Hospedeiro-Patógeno
Imunidade Inata
Inflamassomos/metabolismo
Interleucina-23/metabolismo
Lipoproteínas/metabolismo
Mycoplasma hominis/imunologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/metabolismo
Células Cultivadas
Fracionamento Químico
Células Dendríticas/microbiologia
Detergentes
Eletroforese em Gel de Poliacrilamida
Ensaio de Imunoadsorção Enzimática
Seres Humanos
Lipoproteínas/química
Lipoproteínas/isolamento & purificação
Espectrometria de Massas
Análise em Microsséries
Peso Molecular
Mycoplasma hominis/química
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Detergents); 0 (Inflammasomes); 0 (Interleukin-23); 0 (Lipoproteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170601
[St] Status:MEDLINE


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[PMID]:28291426
[Au] Autor:Sato M; Kubota N; Katsuyama Y; Suzuki Y; Miyairi Y; Minami K; Kasai M
[Ad] Endereço:Departments of 1 Pediatric Intensive Care.
[Ti] Título:Case report of a 6-year-old girl with Mycoplasma hominis ventriculoperitoneal shunt infection.
[So] Source:J Neurosurg Pediatr;19(5):620-624, 2017 May.
[Is] ISSN:1933-0715
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycoplasma hominis is a rare causative pathogen for surgical site infections after neurosurgical procedures. This organism lacks a cell wall, rendering it undetectable by Gram staining and making it resistant to beta-lactam antibiotics. In addition, some special techniques are required to identify this organism. Thus, it is very difficult to diagnose infections caused by this pathogen. Here, the authors report a pediatric case of M. hominis ventriculoperitoneal shunt (VPS) infection with central nervous system involvement for which beta-lactam antibiotics were not effective and Gram staining revealed no pathogens. Because few cases have been described that involve the treatment of M. hominis infection after neurosurgery, in this case the patient's serum and CSF were monitored for antibiotic drug concentrations. Successful treatment of the infection was achieved after approximately 6 weeks of administration of clindamycin and ciprofloxacin antibiotics in addition to external ventricular drain revision and subsequent VPS replacement. When beta-lactam antibiotics are ineffective and when Gram staining cannot detect the responsible pathogens, it is important to consider M. hominis as the atypical pathogen.
[Mh] Termos MeSH primário: Infecções por Mycoplasma/diagnóstico
Infecções por Mycoplasma/terapia
Mycoplasma hominis
Infecções Relacionadas à Prótese/diagnóstico
Infecções Relacionadas à Prótese/terapia
Derivação Ventriculoperitoneal/efeitos adversos
[Mh] Termos MeSH secundário: Criança
Diagnóstico Diferencial
Feminino
Seres Humanos
Hidrocefalia/cirurgia
Infecções por Mycoplasma/líquido cefalorraquidiano
Infecções por Mycoplasma/patologia
Procedimentos Neurocirúrgicos/efeitos adversos
Infecções Relacionadas à Prótese/líquido cefalorraquidiano
Infecções Relacionadas à Prótese/patologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE
[do] DOI:10.3171/2017.1.PEDS16520


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[PMID]:28268197
[Au] Autor:Rowlands S; Danielewski JA; Tabrizi SN; Walker SP; Garland SM
[Ad] Endereço:Department of Maternity Services, Epworth Freemasons Hospital, East Melbourne, Victoria, Australia; Department of Obstetrics and Gynaecology, University of Melbourne, Royal Women's Hospital, Parkville, Victoria, Australia.
[Ti] Título:Microbial invasion of the amniotic cavity in midtrimester pregnancies using molecular microbiology.
[So] Source:Am J Obstet Gynecol;217(1):71.e1-71.e5, 2017 Jul.
[Is] ISSN:1097-6868
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The objective of the study was to determine the frequency of microbial invasion of the amniotic cavity in the midtrimester of pregnancy in patients undergoing amniocentesis for clinical indications. STUDY DESIGN: This was a prospective investigation of the amniotic fluid of 344 asymptomatic women recruited in midpregnancy for the presence of microbial DNA. Amniotic samples obtained at the time of amniocentesis for genetic testing on women between 15 and 22 weeks of gestation were tested specifically for the presence of Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis, and Mycoplasma genitalium as well as for other bacteria and fungi using broad-range polymerase chain reaction only. Pregnancy outcomes were reviewed independent of all molecular test results. RESULTS: Using broad-range polymerase chain reaction, the prevalence of microbial invasion of the amniotic cavity in women between 15 and 22 weeks of gestation was 0% (0 vs 344). Early preterm delivery occurred in only 4 women (1%); 1 delivered electively and 3 spontaneously. None were associated with Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis, or Mycoplasma genitalium. In addition, broad range polymerase chain reaction did not reveal the presence of other bacterial or fungal microbes. CONCLUSION: Microbial invasion of the amniotic cavity in midtrimester gestations of low-risk pregnant women was not detected using molecular methods in 344 patients.
[Mh] Termos MeSH primário: Líquido Amniótico/microbiologia
DNA Bacteriano/análise
Idade Gestacional
[Mh] Termos MeSH secundário: Adulto
Amniocentese/efeitos adversos
Austrália
Feminino
Seres Humanos
Meia-Idade
Mycoplasma genitalium/genética
Mycoplasma genitalium/isolamento & purificação
Mycoplasma hominis/genética
Mycoplasma hominis/isolamento & purificação
Trabalho de Parto Prematuro/microbiologia
Reação em Cadeia da Polimerase
Gravidez
Resultado da Gravidez
Nascimento Prematuro/microbiologia
Estudos Prospectivos
Ureaplasma/genética
Ureaplasma/isolamento & purificação
Ureaplasma urealyticum/genética
Ureaplasma urealyticum/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE


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[PMID]:28257513
[Au] Autor:Otgonjargala B; Becker K; Batbaatar G; Tsogtsaikhan S; Enkhtsetseg J; Enkhjargal A; Pfeffer K; Adams O; Battogtokh C; Henrich B
[Ad] Endereço:Department of Microbiology and Immunology, School of Pharmacy and Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.
[Ti] Título:Effect of Mycoplasma hominis and cytomegalovirus infection on pregnancy outcome: A prospective study of 200 Mongolian women and their newborns.
[So] Source:PLoS One;12(3):e0173283, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Mongolia, diagnostic tests for the detection of the sexually transmitted mycoplasmas, ureaplasmas, Herpes simplex virus (HSV), and cytomegalovirus (CMV) are currently not routinely used in clinical settings and the frequency of these STIs are enigmatic. The prevalence of these STI pathogens were prospectively evaluated among 200 Mongolian pregnant women and their newborns and correlated with pregnancy outcome. TaqMan PCRs were used to detect bacterial and viral STI pathogens in pre-birth vaginal swabs of the pregnant women and in oral swabs of their newborns. A standardized questionnaire concerning former and present pregnancies was developed and linear regression analysis was used to correlate pathogen detection with pregnancy outcome. Ureaplasmas were the most prevalent of the tested pathogens (positive in 90.5% positive women and 47.5% newborns), followed by mycoplasmas (32.5% and 7.5%), chlamydia (14.5% and 7.5%), trichomonas (8.5% and 4.0%) and gonococcus (0.5% and 0%). CMV was found in 46.5% of the pregnant women and in 10.5% of their newborns, whereas HSV-2 was detected in only two mothers. Multiple regression analyses indicate that colonization of the mothers with U. urealyticum, M. hominis, T. vaginalis or CMV is associated with transmission to newborns and that transmission of M. hominis or CMV from Mongolian pregnant women to offspring is associated with reduced neonatal length and gestational age. Thus, diagnostic tests for their detection should be implemented in the clinical settings in Mongolia.
[Mh] Termos MeSH primário: Infecções por Citomegalovirus/epidemiologia
Infecções por Mycoplasma/epidemiologia
Complicações Infecciosas na Gravidez/epidemiologia
[Mh] Termos MeSH secundário: Citomegalovirus/isolamento & purificação
Citomegalovirus/patogenicidade
Infecções por Citomegalovirus/complicações
Infecções por Citomegalovirus/virologia
Feminino
Seres Humanos
Recém-Nascido
Mongólia/epidemiologia
Infecções por Mycoplasma/complicações
Infecções por Mycoplasma/microbiologia
Mycoplasma hominis/isolamento & purificação
Mycoplasma hominis/patogenicidade
Gravidez
Complicações Infecciosas na Gravidez/microbiologia
Complicações Infecciosas na Gravidez/fisiopatologia
Complicações Infecciosas na Gravidez/virologia
Resultado da Gravidez
Estudos Prospectivos
Doenças Sexualmente Transmissíveis/complicações
Doenças Sexualmente Transmissíveis/diagnóstico
Doenças Sexualmente Transmissíveis/microbiologia
Doenças Sexualmente Transmissíveis/virologia
Inquéritos e Questionários
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173283


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[PMID]:28141596
[Au] Autor:D'Inzeo T; De Angelis G; Fiori B; Menchinelli G; Liotti FM; Morandotti GA; De Maio F; Nagel D; Antonaci M; Sanguinetti M; Spanu T
[Ad] Endereço:Institute of Microbiology, Catholic University, Rome, Italy. tiziana.dinzeo@rm.unicatt.it.
[Ti] Título:Comparison of Mycoplasma IES, Mycofast Revolution and Mycoplasma IST2 to detect genital mycoplasmas in clinical samples.
[So] Source:J Infect Dev Ctries;11(1):98-101, 2017 Jan 30.
[Is] ISSN:1972-2680
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Culture is regarded as the gold standard for the detection of genital mycoplasma in clinical samples. Commercially available diagnostic kits, based on liquid broth cultures, provide interesting alternatives to conventional culture. We assessed the laboratory performances of Mycoplasma IES (IES), the Mycofast Revolution (REV) and Mycoplasma IST 2 (IST2) compared to A7 agar plates for the detection of Ureaplasma urealyticum and Mycoplasma hominis in clinical samples. METHODOLOGY: From April to July 2013, endocervical or vaginal samples were collected from sexually active women with abnormal vaginal discharge. Each specimen was tested in parallel using the three commercial kits and the A7 agar plates. RESULTS: A total of 303 samples were included in this study, 35.6% (108/303) of which were positive on A7 plates. Sensitivities for the detection of U. urealyticum of IES, REV and IST2 were 100%, 96.2% and 95.3%, respectively while those for M. hominis were of 92.8%, 92.8% and 85.7%, respectively. Specificity was 100% for the 3 methods. Concerning antimicrobial susceptibility testing, full agreement between IES and REV was documented. CONCLUSIONS: The Mycoplasma IES kit was found to be equivalent or superior compared to other commercial culture-based assays for a rapid and accurate identification of U. urealyticum and M. hominis and detection of resistance. It might be considered a cost-effective tool for detection of these organisms, particularly attractive in developing countries.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas/métodos
Testes Diagnósticos de Rotina/métodos
Infecções por Mycoplasma/diagnóstico
Mycoplasma hominis/isolamento & purificação
Infecções do Sistema Genital/diagnóstico
Infecções por Ureaplasma/diagnóstico
Ureaplasma urealyticum/isolamento & purificação
[Mh] Termos MeSH secundário: Colo do Útero/microbiologia
Custos e Análise de Custo
Feminino
Seres Humanos
Estudos Prospectivos
Kit de Reagentes para Diagnóstico
Sensibilidade e Especificidade
Fatores de Tempo
Vagina/microbiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Reagent Kits, Diagnostic)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.3855/jidc.8039


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[PMID]:28069785
[Au] Autor:Bergin SM; Mendis SM; Young B; Binti Izharuddin E
[Ad] Endereço:Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore, Singapore.
[Ti] Título:Postoperative Mycoplasma hominis brain abscess: keep it in mind!
[So] Source:BMJ Case Rep;2017, 2017 Jan 09.
[Is] ISSN:1757-790X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A temporal lobe abscess was diagnosed in a 57-year-old man. A urethral catheter had been inserted 12 days earlier, just prior to clot evacuation of a subacute haematoma secondary to an arterio-venous malformation. Fever persisted despite debridement and treatment with meropenem and vancomycin. Gram stains of operative samples showed no bacteria. Extended cultures grew pinpoint colonies after 5 days. Meanwhile, sequencing of bacterial 16S rDNA from operative specimens had identified Mycoplasma hominis; the bacterial colonies were subsequently similarly identified. The patient responded promptly following addition of oral doxycycline 100 mg two times per day. There is a growing literature of similar cases. Transient bacteraemia, following urinary catheterisation, with seeding of existing sites of inflammation is the proposed explanation. Urethral carriage of M. hominis is 15% and catheterisation is a common procedure. Mycoplasma hominis maybe more common than appreciated, especially as the need for extended cultures makes a correct diagnosis less likely.
[Mh] Termos MeSH primário: Abscesso Encefálico/etiologia
Infecções por Mycoplasma/diagnóstico
Mycoplasma hominis
Complicações Pós-Operatórias/diagnóstico por imagem
Cateterismo Urinário/efeitos adversos
[Mh] Termos MeSH secundário: Abscesso Encefálico/diagnóstico por imagem
Abscesso Encefálico/cirurgia
DNA Bacteriano/isolamento & purificação
Hematoma Subdural Agudo/cirurgia
Seres Humanos
Malformações Arteriovenosas Intracranianas/complicações
Malformações Arteriovenosas Intracranianas/cirurgia
Masculino
Meia-Idade
Mycoplasma hominis/genética
Complicações Pós-Operatórias/cirurgia
Lobo Temporal/diagnóstico por imagem
Tomografia Computadorizada por Raios X
Uretra
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170111
[St] Status:MEDLINE


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[PMID]:27881098
[Au] Autor:Jironkin A; Brown RJ; Underwood A; Chalker VJ; Spiller OB
[Ad] Endereço:Colindale, Public Health England, London, UK. Aleksey.Jironkin@phe.gov.uk.
[Ti] Título:Genomic determination of minimum multi-locus sequence typing schemas to represent the genomic phylogeny of Mycoplasma hominis.
[So] Source:BMC Genomics;17(1):964, 2016 11 23.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mycoplasma hominis is an opportunistic human pathogen, associated with clinically diverse disease. Currently, there is no standardised method for typing M. hominis, which would aid in understanding pathogen epidemiology and transmission. Due to availability and costs of whole genome sequencing and the challenges in obtaining adequate M. hominis DNA, the use of whole genome sequence analysis to provide clinical guidance is unpractical for this bacterial species as well as other fastidious organisms. RESULTS: This study identified pan-genome set of 700 genes found to be present in four published reference genomes. A subset of 417 genes was identified to be core genome for 18 isolates and 1 reference. Leave-one-out analysis of the core genes highlighted set of 48 genes that are required to recapture the original phylogenetic relationships observed using whole genome SNP analysis. Three 7-locus MLST schemas with high diversity index (97%) and low dN/dS ratios (0.1, 0.13, and 0.11) were derived that could be used to confer good discrimination between strains and could be of practical use in future studies direct on clinical specimens. CONCLUSIONS: The genes proposed in this study could be utilised to design a cost-effective and rapid PCR-based MLST assay that could be applied directly to clinical isolates, without prior isolation. This study includes additional genomic analysis revealing high levels of genetic heterogeneity among this species. This provides a novel and evidence based approach for the development of MLST schema that accurately represent genomic phylogeny for use in epidemiology and transmission studies.
[Mh] Termos MeSH primário: Genoma Bacteriano
Genômica
Mycoplasma hominis/classificação
Mycoplasma hominis/genética
[Mh] Termos MeSH secundário: Alelos
Análise por Conglomerados
Genes Bacterianos
Genômica/métodos
Genótipo
Seres Humanos
Tipagem de Sequências Multilocus
Mycoplasma hominis/isolamento & purificação
Filogenia
Polimorfismo de Nucleotídeo Único
Recombinação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161125
[St] Status:MEDLINE



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