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[PMID]:28748939
[Au] Autor:Perkel JM
[Ad] Endereço:Nature.
[Ti] Título:Cell engineering: How to hack the genome.
[So] Source:Nature;547(7664):477-479, 2017 07 26.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Engenharia Celular
Engenharia Genética
Genoma/genética
Biologia Sintética/tendências
[Mh] Termos MeSH secundário: Animais
Cromatina/genética
Cromatina/metabolismo
Cromossomos Artificiais de Levedura/genética
DNA/biossíntese
DNA/genética
DNA/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Redes Reguladoras de Genes/genética
Seres Humanos
Mycoplasma mycoides/genética
Saccharomyces cerevisiae/genética
Salmonella typhimurium/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 9007-49-2 (DNA)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1038/547477a


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[PMID]:28332650
[Au] Autor:Almaw G; Duguma M; Wubetie A; Tuli G; Koran T
[Ti] Título:A contagious bovine pleuropneumonia outbreak on a research farm in Ethiopia, and its dynamics over an eight-month period.
[So] Source:Rev Sci Tech;35(3):787-793, 2016 Dec.
[Is] ISSN:0253-1933
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Contagious bovine pleuropneumonia (CBPP) was recognised on Bako Agricultural Research Farm, in the Oromia Region of Ethiopia, for the first time on 5 May 2011. The outbreak was investigated by combining recognition of clinical signs, post-mortem examination, mycoplasma isolation and serological testing using competitive enzymelinked immunosorbent assay (c-ELISA). The clinical cases were monitored for eight months; sick animals were treated with a range of antibiotics and isolated if necessary. The outbreak of CBPP was confirmed both bacteriologically and serologically and had spread to almost the entire herd (96.7%) within the eight-month observation period. Of the animals that recovered after antibiotic treatment, 12.3% fell sick again, showed typical signs of CBPP and were considered to be carriers. The role of treatment in the prevention of the spread of CBPP was minimal. Newly purchased animals that were not tested and quarantined before being introduced onto the farm were suspected to have been the most probable source of infection.
[Mh] Termos MeSH primário: Antibacterianos/administração & dosagem
Doenças dos Bovinos/epidemiologia
Surtos de Doenças/veterinária
Mycoplasma mycoides/imunologia
Pleuropneumonia Contagiosa/epidemiologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Bovinos
Doenças dos Bovinos/tratamento farmacológico
Doenças dos Bovinos/microbiologia
Etiópia/epidemiologia
Feminino
Pulmão/patologia
Masculino
Mycoplasma/crescimento & desenvolvimento
Mycoplasma/isolamento & purificação
Oxitetraciclina/administração & dosagem
Penicilinas/administração & dosagem
Pleuropneumonia Contagiosa/tratamento farmacológico
Estreptomicina/administração & dosagem
Tilosina/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antibodies, Bacterial); 0 (Penicillins); X20I9EN955 (Oxytetracycline); Y45QSO73OB (Streptomycin); YEF4JXN031 (Tylosin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.20506/rst.35.3.2569


  3 / 582 MEDLINE  
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[PMID]:27649681
[Au] Autor:Kama-Kama F; Midiwo J; Nganga J; Maina N; Schiek E; Omosa LK; Osanjo G; Naessens J
[Ad] Endereço:Department of Biochemistry, Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000, Nairobi, Kenya; Biosciences Eastern & Central Africa - International Livestock Research Institute, Old Naivasha Road, PO Box 30709, 00100 Nairobi, Kenya. Electronic address: franciscayeye@yahoo.fr
[Ti] Título:Selected ethno-medicinal plants from Kenya with in vitro activity against major African livestock pathogens belonging to the "Mycoplasma mycoides cluster".
[So] Source:J Ethnopharmacol;192:524-534, 2016 Nov 04.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMOCOLOGICAL RELEVANCE: Members of 'Mycoplasma mycoides cluster' are important ruminant pathogens in Africa. Diseases caused by these Mycoplasma negatively affect the agricultural sector especially in developing countries through losses in livestock productivity, mortality and international trade restrictions. There is therefore urgent need to develop antimicrobials from alternative sources such as medicinal plants to curb these diseases. In Kenya, smallholder farmers belonging to the Maasai, Kuria and Luo rely on traditional Kenyan herbals to treat respiratory symptoms in ruminants. In the current study extracts from some of these plants were tested against the growth of members of Mycoplasma mycoides cluster. AIM: This study aimed at identifying plants that exhibit antimycoplasmal activities using an ethnobotanical approach. MATERIALS AND METHODS: Kenyan farmers of Maasai, Luo and Kuria ethnic groups were interviewed for plant remedies given to livestock with respiratory syndromes. The plant materials were thereafter collected and crude extracts prepared using a mixture of 50% of methanol (MeOH) in dichloromethane (CH Cl ), neat methanol (MeOH), ethanol (EtOH) and water to yield four crude extracts per plant part. The extracts were tested in vitro against five strains of Mycoplasma mycoides subsp. capri, five strains of Mycoplasma mycoides subsp. mycoides and one strain of Mycoplasma capricolum subsp capricolum using broth micro-dilution assays with an initial concentration of 1mg/ml. Minimum inhibitory concentration (MIC) of the most active extracts were determined by serial dilution. RESULTS: Extracts from five plants namely: Solanum aculeastrum, Albizia coriaria, Ekebergia capensis, Piliostigma thonningii and Euclea divinorum exhibited the highest activities against the Mycoplasma strains tested. Mycoplasma mycoides subsp. mycoides were more susceptible to these extracts than Mycoplasma mycoides subsp. capri and Mycoplasma capricolum susp. capricolum. The activities of the crude extracts varied with the solvent used for extraction. The MICs mean values of the active extracts varied from 0.02 to 0.6mg/ml. CONCLUSIONS: The results suggested that these plants could potentially contain antimicrobial compounds that might be useful for the treatment of respiratory diseases in ruminants. Future work should focus on the isolation and identification of the active compounds from the plant extracts that showed interesting activities and evaluation of their antimicrobial and cytotoxic potential.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Gado/microbiologia
Mycoplasma mycoides/efeitos dos fármacos
Extratos Vegetais/farmacologia
Plantas Medicinais/química
Pleuropneumonia Contagiosa/tratamento farmacológico
Drogas Veterinárias/farmacologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/isolamento & purificação
Etnobotânica
Etnofarmacologia
Fazendeiros
Entrevistas como Assunto
Quênia
Testes de Sensibilidade Microbiana
Fitoterapia/veterinária
Extratos Vegetais/isolamento & purificação
Pleuropneumonia Contagiosa/microbiologia
Solventes/química
Drogas Veterinárias/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Plant Extracts); 0 (Solvents); 0 (Veterinary Drugs)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160922
[St] Status:MEDLINE


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[PMID]:27496744
[Au] Autor:Mwirigi M; Nkando I; Olum M; Attah-Poku S; Ochanda H; Berberov E; Potter A; Gerdts V; Perez-Casal J; Wesonga H; Soi R; Naessens J
[Ad] Endereço:Kenya Agricultural and Livestock Research Organization, Biotechnology Research Institute, P.O. Box 14733, 00800, Nairobi, Kenya. Electronic address: martin.mwirigi@kalro.org.
[Ti] Título:Capsular polysaccharide from Mycoplasma mycoides subsp. mycoides shows potential for protection against contagious bovine pleuropneumonia.
[So] Source:Vet Immunol Immunopathol;178:64-9, 2016 Oct 01.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Contagious Bovine Pleuropneumonia (CBPP) is a severe respiratory disease caused by Mycoplasma mycoides subsp. mycoides (Mmm) which is widespread in Africa. The capsule polysaccharide (CPS) of Mmm is one of the few identified virulence determinants. In a previous study, immunization of mice against CPS generated antibodies, but they were not able to prevent multiplication of Mmm in this model animal. However, mice cannot be considered as a suitable animal model, as Mmm does not induce pathology in this species. Our aim was to induce antibody responses to CPS in cattle, and challenge them when they had specific CPS antibody titres similar or higher than those from cattle vaccinated with the live vaccine. The CPS was linked to the carrier protein ovalbumin via a carbodiimide-mediated condensation with 1-ethyl-3(3-imethylaminopropyl) carbodiimide (EDC). Ten animals were immunized twice and challenged three weeks after the booster inoculation, and compared to a group of challenged non-immunized cattle. When administered subcutaneously to adult cattle, the vaccine elicited CPS-specific antibody responses with the same or a higher titre than animals vaccinated with the live vaccine. Pathology in the group of immunized animals was significantly reduced (57%) after challenge with Mmm strain Afadé compared to the non-immunized group, a figure in the range of the protection provided by the live vaccine.
[Mh] Termos MeSH primário: Cápsulas Bacterianas/imunologia
Doenças dos Bovinos/prevenção & controle
Mycoplasma mycoides/imunologia
Pleuropneumonia Contagiosa/prevenção & controle
Polissacarídeos Bacterianos/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Vacinas Bacterianas/administração & dosagem
Vacinas Bacterianas/imunologia
Bovinos
Doenças dos Bovinos/imunologia
Imunização Secundária/veterinária
Camundongos
Pleuropneumonia Contagiosa/imunologia
Vacinação/veterinária
Vacinas Conjugadas/administração & dosagem
Vacinas Conjugadas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Bacterial Vaccines); 0 (Polysaccharides, Bacterial); 0 (Vaccines, Conjugate)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160807
[St] Status:MEDLINE


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[PMID]:27488189
[Au] Autor:Labroussaa F; Lebaudy A; Baby V; Gourgues G; Matteau D; Vashee S; Sirand-Pugnet P; Rodrigue S; Lartigue C
[Ad] Endereço:INRA, UMR 1332 de Biologie du Fruit et Pathologie, F-33140 Villenave d'Ornon, France University of Bordeaux, UMR 1332 de Biologie du Fruit et Pathologie, F-33140 Villenave d'Ornon, France.
[Ti] Título:Impact of donor-recipient phylogenetic distance on bacterial genome transplantation.
[So] Source:Nucleic Acids Res;44(17):8501-11, 2016 Sep 30.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Genome transplantation (GT) allows the installation of purified chromosomes into recipient cells, causing the resulting organisms to adopt the genotype and the phenotype conferred by the donor cells. This key process remains a bottleneck in synthetic biology, especially for genome engineering strategies of intractable and economically important microbial species. So far, this process has only been reported using two closely related bacteria, Mycoplasma mycoides subsp. capri (Mmc) and Mycoplasma capricolum subsp. capricolum (Mcap), and the main factors driving the compatibility between a donor genome and a recipient cell are poorly understood. Here, we investigated the impact of the evolutionary distance between donor and recipient species on the efficiency of GT. Using Mcap as the recipient cell, we successfully transplanted the genome of six bacteria belonging to the Spiroplasma phylogenetic group but including species of two distinct genera. Our results demonstrate that GT efficiency is inversely correlated with the phylogenetic distance between donor and recipient bacteria but also suggest that other species-specific barriers to GT exist. This work constitutes an important step toward understanding the cellular factors governing the GT process in order to better define and eventually extend the existing genome compatibility limit.
[Mh] Termos MeSH primário: Genoma Bacteriano
Mycoplasma capricolum/genética
Mycoplasma mycoides/genética
Filogenia
Transformação Genética
[Mh] Termos MeSH secundário: Clonagem Molecular
Replicação do DNA/genética
DNA Bacteriano/genética
Marcadores Genéticos
Genótipo
Mutagênese Insercional/genética
Fenótipo
Plasmídeos/metabolismo
Reprodutibilidade dos Testes
Saccharomyces cerevisiae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Genetic Markers)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160805
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkw688


  6 / 582 MEDLINE  
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[PMID]:27387734
[Au] Autor:Paterna A; Tatay-Dualde J; Amores J; Prats-van der Ham M; Sánchez A; de la Fe C; Contreras A; Corrales JC; Gómez-Martín Á
[Ad] Endereço:Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence 'Campus Mare Nostrum', Universidad de Murcia, Campus de Espinardo s/n., 30100 Murcia, Spain.
[Ti] Título:In vitro assessment of the antimicrobial susceptibility of caprine isolates of Mycoplasma mycoides subsp. capri.
[So] Source:Vet J;214:96-101, 2016 Aug.
[Is] ISSN:1532-2971
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The minimum inhibitory concentration (MIC) and minimum mycoplasmacidal concentration (MMC) of 17 antimicrobials against 41 Spanish caprine isolates of Mycoplasma mycoides subsp. capri (Mmc) obtained from different specimens (milk, external auricular canal and semen) were determined using a liquid microdilution method. For half of the isolates, the MIC was also estimated for seven of the antimicrobials using an epsilometric test (ET), in order to compare both methods and assess the validity of ET. Mutations in genes gyrA, gyrB, parC and parE conferring fluoroquinolone resistance, which have been recently described in Mmc, were investigated using PCR. The anatomical origin of the isolate had no effect on its antimicrobial susceptibility. Moxifloxacin and doxycycline had the lowest MIC values. The rest of the fluoroquinolones studied (except norfloxacin), together with tylosin and clindamycin, also had low MIC values, although the MMC obtained for clindamycin was higher than for the other antimicrobials. For all the aminoglycosides, spiramycin and erythromycin, a notable level of resistance was observed. The ET was in close agreement with broth microdilution at low MICs, but not at intermediate or high MICs. The analysis of the genomic sequences revealed the presence of an amino acid substitution in codon 83 of the gene gyrA, which has not been described previously in Mmc.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana
Doenças das Cabras/tratamento farmacológico
Mycoplasma mycoides/efeitos dos fármacos
Pleuropneumonia Contagiosa/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Meato Acústico Externo/microbiologia
Feminino
Doenças das Cabras/microbiologia
Cabras
Masculino
Testes de Sensibilidade Microbiana/veterinária
Leite/microbiologia
Pleuropneumonia Contagiosa/microbiologia
Espanha
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160709
[St] Status:MEDLINE


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[PMID]:27374908
[Au] Autor:Tatay-Dualde J; Prats-van der Ham M; de la Fe C; Gómez-Martín Á; Paterna A; Corrales JC; Contreras A; Sánchez A
[Ad] Endereço:Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Campus de Espinardo s/n., 30100 Murcia, Spain.
[Ti] Título:Multilocus sequence typing of Mycoplasma mycoides subsp. capri to assess its genetic variability in a contagious agalactia endemic area.
[So] Source:Vet Microbiol;191:60-4, 2016 Aug 15.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mycoplasma mycoides subsp. capri (Mmc) is one of the main causative agents of caprine contagious agalactia. Besides, the absence of accurate control methods eases its dispersion between different herds within endemic areas of this disease. In this context, there is a need to implement molecular typing schemes which offer valuable information useful to establish control measures and enables the surveillance of this pathogen. The aim of this study was to assess the genetic variability of different strains of Mmc from a contagious agalactia endemic area through multilocus sequence typing (MLST). For this purpose, five house-keeping genes (fusA, glpQ, gyrB, lepA, rpoB) from 39 field isolates were analysed. These isolates were obtained from different geographic areas of Spain, between the years 2004 and 2015. The results obtained in this study suggest that the selected MLST scheme could be a useful technique to monitor the genetic variability of Mmc in endemic areas. Despite the significant differences found between the assessed field isolates, they could be classified according to their geographical origin. Moreover, it was also possible to detect genetic differences between Mmc strains coming from the same herd at the same sampling time, which may need to be taken into consideration when designing or arranging prophylactic strategies.
[Mh] Termos MeSH primário: Variação Genética
Doenças das Cabras/microbiologia
Infecções por Mycoplasma/veterinária
Mycoplasma mycoides/genética
[Mh] Termos MeSH secundário: Animais
Doenças Endêmicas
Genes Bacterianos/genética
Doenças das Cabras/epidemiologia
Cabras
Tipagem de Sequências Multilocus
Infecções por Mycoplasma/epidemiologia
Infecções por Mycoplasma/microbiologia
Mycoplasma mycoides/classificação
Filogenia
Espanha/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170210
[Lr] Data última revisão:
170210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160705
[St] Status:MEDLINE


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[PMID]:27247066
[Au] Autor:Daniel G; Abdurahaman M; Tuli G; Deresa B
[Ti] Título:Contagious bovine pleuropneumonia: Seroprevalence and risk factors in Western Oromia, Ethiopia.
[So] Source:Onderstepoort J Vet Res;83(1):a958, 2016 May 12.
[Is] ISSN:2219-0635
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:Contagious bovine pleuropneumonia (CBPP) is one of the most important threats to cattle health and production in Ethiopia. At the livestock farm of the Bako Agricultural Research Center, an outbreak of respiratory disease of cattle occurred in May 2011, and many animals were affected and died before the disease was diagnosed. Therefore, this study was designed to determine the seroprevalence of CBPP antibodies in selected districts of Western Oromia Region and to assess the potential risk factors for the occurrence of the disease. A crosssectional study was conducted from November 2013 to March 2014 in three selected districts of Western Oromia Region. A total of 386 sera were examined for the presence of specific antibodies against Mycoplasma mycoidesmycoides small colony (MmmSC), using a competitive enzyme-linked immunosorbent assay. The risk factors that were evaluated in this study were geographical location, age, sex, breed and body condition. The overall seroprevalence in this study was 28.5%. The seroprevalence of Mycoplasma mycoidesmycoides small colony antibodies at the district level was 40.3%, 19.0% and 5.7% in Gobbu Sayyo, BakoTibbe and Horro districts, respectively. There was a statistically significant variation ( p < 0.05) in the prevalence of antibodies amongst the districts. However, animal-related risk factors, such as age, sex, breed and body condition, were not significantly associated ( p > 0.05) with the serological status of the animal. This study showed that the overall prevalence of CBPP in Western Oromia Zones was high. This warrants the implementation of appropriate preventive and control measures to minimise the economic losses associated with the disease.
[Mh] Termos MeSH primário: Doenças dos Bovinos/epidemiologia
Surtos de Doenças/veterinária
Mycoplasma mycoides/isolamento & purificação
Pleuropneumonia Contagiosa/epidemiologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Bovinos
Doenças dos Bovinos/microbiologia
Estudos Transversais
Ensaio de Imunoadsorção Enzimática/veterinária
Etiópia/epidemiologia
Feminino
Masculino
Pleuropneumonia Contagiosa/microbiologia
Prevalência
Fatores de Risco
Estudos Soroepidemiológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160602
[St] Status:MEDLINE
[do] DOI:10.4102/ojvr.v83i1.958


  9 / 582 MEDLINE  
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[PMID]:27237398
[Au] Autor:Alhaji NB; Babalobi OO
[Ad] Endereço:Department of Veterinary Public Health and Preventive Medicine, University of Ibadan, Ibadan, Nigeria; Zoonoses and Epidemiology Unit, Niger State Ministry of Livestock and Fisheries Development, Minna, Nigeria. Electronic address: nmabida62@gmail.com.
[Ti] Título:Qualitative and quantitative impacts assessment of contagious bovine pleuropneumonia in Fulani pastoral herds of North-central Nigeria: The associated socio-cultural factors.
[So] Source:Prev Vet Med;128:124-34, 2016 Jun 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Contagious bovine pleuropneumonia is one of the most important trans-boundary disease affecting Fulani cattle herds of Nigeria and whose control is urgently needed. A Participatory Epidemiology approach and cross-sectional study were concurrently conducted to investigate qualitative and quantitative impacts of CBPP, respectively and associated socio-cultural factors that influenced exposure of Fulani nomadic pastoral communities to its risk in Niger State, North-central Nigeria between January and December 2013. A total of nine pastoral communities were purposively selected for qualitative impact assessment using Participatory Rural Appraisal tools, while 765 cattle randomly sampled from 125 purposively selected nomadic herds were analyzed using c-ELISA. Data on socio-cultural characteristics were collected using structured questionnaires administered on nomadic herd owners of the 125 selected herds. Kendall's Coefficient of Concordance W statistics and OpenEpi 2.3 were used for statistical analyses. Pastoralists' dependent factors associated with their socio-cultural activities were tested using Chisquare tests and likelihood backward logistic regressions. The mean proportional piles (relative qualitative impact) of CBPP was 12.6%, and nomads agreement on this impact was strong (W=0.6855) and statistically significant (P<0.001). This was validated by 16.2% (95% CI: 13.7, 19.0) sero-positive (quantitative impact). Highest sero-prevalence of 25.3% was observed in Northern agro-ecological zone, while lowest of 6.2% was in Eastern zone. Pastoralists in the age groups 51-60 and 61-70 years were more likely (OR 13.07; 95% CI: 3.21, 53.12 and OR 7.10; 95% CI: 1.77, 28.33, respectively) to have satisfactory information/awareness on CBPP and lowland transhumance pastoralists were more likely (OR 5.21; 95% CI: 2.01, 13.54) to have satisfactory information. Socio-cultural activities of extensive husbandry system was six times more likely (OR 5.79; 95% CI: 2.55, 13.13) to be satisfactory practice that influenced CBPP occurrence in herds, while culture of borrowing and loaning of cattle was twenty times more likely (OR 19.94; 95% CI: 6.36, 62.48) to be satisfactory practice that influenced CBPP occurrence in herds. Also, sharing a water source that caused concentration of stocks in one point was fifty three times more likely (OR 53.08; 95% CI: 14.91, 189.00) to be satisfactory practice that influenced occurrence of the disease in herds. This study highlighted the critical gap that exists in terms of significant influence of socio-cultural factors on CBPP occurrence in pastoral herds in Nigeria. Thus, CBPP surveillance, control and prevention programs that take these factors into consideration will be beneficial to the livestock industry in Nigeria, and indeed Africa.
[Mh] Termos MeSH primário: Doenças dos Bovinos/epidemiologia
Mycoplasma mycoides/fisiologia
Pleuropneumonia Contagiosa/epidemiologia
[Mh] Termos MeSH secundário: Criação de Animais Domésticos
Animais
Bovinos
Doenças dos Bovinos/virologia
Estudos Transversais
Ensaio de Imunoadsorção Enzimática/veterinária
Modelos Logísticos
Nigéria/epidemiologia
Pleuropneumonia Contagiosa/virologia
Prevalência
Estudos Soroepidemiológicos
Fatores Socioeconômicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160531
[St] Status:MEDLINE


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[PMID]:27114507
[Au] Autor:Arfi Y; Minder L; Di Primo C; Le Roy A; Ebel C; Coquet L; Claverol S; Vashee S; Jores J; Blanchard A; Sirand-Pugnet P
[Ad] Endereço:INRA (Institut National de la Recherche Agronomique), UMR 1332 Biologie du Fruit et Pathologie, F-33882 Villenave d'Ornon, France; University of Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, F-33882 Villenave d'Ornon, France; yonathan.arfi@u-bordeaux.fr.
[Ti] Título:MIB-MIP is a mycoplasma system that captures and cleaves immunoglobulin G.
[So] Source:Proc Natl Acad Sci U S A;113(19):5406-11, 2016 May 10.
[Is] ISSN:1091-6490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycoplasmas are "minimal" bacteria able to infect humans, wildlife, and a large number of economically important livestock species. Mycoplasma infections include a spectrum of clinical manifestations ranging from simple fever to fulminant inflammatory diseases with high mortality rates. These infections are mostly chronic, suggesting that mycoplasmas have developed means to evade the host immune response. Here we present and functionally characterize a two-protein system from Mycoplasma mycoides subspecies capri that is involved in the capture and cleavage of IgG. The first component, Mycoplasma Ig binding protein (MIB), is an 83-kDa protein that is able to tightly bind to the Fv region of a wide range of IgG. The second component, Mycoplasma Ig protease (MIP), is a 97-kDa serine protease that is able to cleave off the VH domain of IgG. We demonstrate that MIB is necessary for the proteolytic activity of MIP. Cleavage of IgG requires a sequential interaction of the different partners of the system: first MIB captures the IgG, and then MIP is recruited to the MIB-IgG complex, enabling protease activity. MIB and MIP are encoded by two genes organized in tandem, with homologs found in the majority of pathogenic mycoplasmas and often in multiple copies. Phylogenetic studies suggest that genes encoding the MIB-MIP system are specific to mycoplasmas and have been disseminated by horizontal gene transfer. These results highlight an original and complex system targeting the host immunoglobulins, playing a potentially key role in the immunity evasion by mycoplasmas.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Imunoglobulina G/metabolismo
Complexos Multiproteicos/metabolismo
Mycoplasma mycoides/metabolismo
[Mh] Termos MeSH secundário: Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Immunoglobulin G); 0 (Multiprotein Complexes)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160427
[St] Status:MEDLINE
[do] DOI:10.1073/pnas.1600546113



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