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[PMID]:28470277
[Au] Autor:Tseng TS; Tsai KC; Chen C
[Ad] Endereço:Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan. bmchinp@ibms.sinica.edu.tw.
[Ti] Título:Characterizing the structure-function relationship reveals the mode of action of a novel antimicrobial peptide, P1, from jumper ant Myrmecia pilosula.
[So] Source:Mol Biosyst;13(6):1193-1201, 2017 Jun 01.
[Is] ISSN:1742-2051
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microbial infections of antibiotic-resistant strains cause serious diseases and have a significant impact on public health worldwide, so novel antimicrobial drugs are urgently needed. Insect venoms, a rich source of bioactive components containing antimicrobial peptides (AMPs), are attractive candidates for new therapeutic agents against microbes. Recently, a novel peptide, P1, identified from the venom of the Australian jumper ant Myrmecia pilosula, showed potent antimicrobial activities against both Gram-negative and Gram-positive bacteria, but its structure-function relationship is unknown. Here, we used biochemical and biophysical techniques coupled with computational simulations to explore the mode of action of P1 interaction with dodecylphosphocholine (DPC) micelles as a model membrane system. Our circular dichroism (CD) and NMR studies revealed an amphipathic α-helical structure for P1 upon interaction with DPC micelles. A paramagnetic relaxation enhancement approach revealed that P1 orients its α-helix segment (F6-G14) into DPC micelles. In addition, the α-helix segment could be essential for membrane permeabilization and antimicrobial activity. Moreover, the arginine residues R8, R11, and R15 significantly contribute to helix formation and membrane-binding affinity. The lysine residue K19 of the C-terminus functionally guides P1 to interact with DPC micelles in the early interaction stage. Our study provides insights into the mode of action of P1, which is valuable in modifying and developing potent AMPs as antibiotic drugs.
[Mh] Termos MeSH primário: Anti-Infecciosos/química
Anti-Infecciosos/farmacologia
Biologia Computacional
[Mh] Termos MeSH secundário: Peptídeos Catiônicos Antimicrobianos/química
Peptídeos Catiônicos Antimicrobianos/farmacologia
Dicroísmo Circular
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Espectroscopia de Ressonância Magnética
Micelas
Testes de Sensibilidade Microbiana
Estrutura Secundária de Proteína
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antimicrobial Cationic Peptides); 0 (Micelles)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1039/c6mb00810k


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[PMID]:29458541
[Au] Autor:Kim JH; Kim TS; Song SH; Choi J; Han S; Kim DY; Kwon S; Lee E; Song KH; Choe PG; Bang JH; Kim ES; Park SW; Kim HB; Kim NJ; Park WB; Oh MD
[Ad] Endereço:1​Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.
[Ti] Título:Direct rapid antibiotic susceptibility test (dRAST) for blood culture and its potential usefulness in clinical practice.
[So] Source:J Med Microbiol;67(3):325-331, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The direct rapid antibiotic susceptibility test (dRAST), based on analysing changes in bacterial micro-colonies under antibiotic conditions, detects antibiotic resistance within 6 h of direct smear examination results. This study aimed to assess the accuracy of dRAST and evaluate its potential usefulness for improving selection of appropriate antibiotic in real clinical practice settings. METHODOLOGY: We evaluated the accuracy of dRAST by comparing the antibiotic treatments that should have been administered based on dRAST results and the broth microdilution (BMD) test and its potential usefulness via simulation. RESULT: For 49/52 (94.2 %) patients with Gram-positive bacteraemia and 66/67 (98.5 %) patients with Gram-negative bacteraemia, antibiotics indicated by dRAST results were the same as those indicated by the BMD test. Among 34 patients with ineffective and suboptimal treatment, 19 (55.9 %) of patients could have received optimal treatment 1 to 2 days earlier with dRAST results. Among 33 patients given unnecessary broad-spectrum antibiotics, 1 to 2 days earlier de-escalation could have been possible for 27 (81.8 %) patients based on dRAST results. CONCLUSION: The introduction of dRAST could increase the use of optimal antibiotics and reduce unnecessary broad-spectrum antibiotic use in the early period of bacteraemia.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Hemocultura
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Testes de Sensibilidade Microbiana/métodos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Bacteriemia/tratamento farmacológico
Bacteriemia/microbiologia
Acurácia dos Dados
Feminino
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Positivas/isolamento & purificação
Seres Humanos
Masculino
Meia-Idade
Estudos Retrospectivos
Adulto Jovem
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000678


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[PMID]:29340524
[Au] Autor:Baraba A; Kqiku L; Gabric D; Verzak Z; Hanscho K; Miletic I
[Ad] Endereço:Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
[Ti] Título:Efficacy of removal of cariogenic bacteria and carious dentin by ablation using different modes of Er:YAG lasers.
[So] Source:Braz J Med Biol Res;51(3):e6872, 2018 Jan 11.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The primary objective of this in vitro study was to evaluate the efficiency of removal of cariogenic bacteria and carious dentin by ablation using two lasers: fluorescence-feedback controlled (FFC) Er:YAG laser and different pulses of Er:YAG laser based on variable square pulse technology (VSPt). The secondary objective was to measure the temperature during laser ablation of carious tissue. Seventy-two extracted human molars were used in this study. Sixty teeth with carious dentin were randomly divided into four experimental groups according to the treatment for caries removal: group 1: 400 µs (FFC group); group 2: super short pulse (SSP group, 50 µs pulse); group 3: medium short pulse (MSP group, 100 µs pulse); group 4: short pulse (SP group, 300 µs pulse) and one positive control group with no treatment. Twelve teeth without carious lesion were used as a negative control group. After caries removal, swabs were taken with cotton pellets and real-time PCR analysis was performed. During caries ablation, a thermal infrared camera was used to measure the temperature changes. In all experimental groups, specimens were free of bacterial contamination after the treatment. In the SSP, MSP and SP groups, temperatures measured during caries ablation were significantly higher compared to temperatures in the FFC group (P<0.001). In this in vitro study, laser treatment for removal of carious dentin and cariogenic bacteria was an efficient treatment modality without causing excessive temperatures that might adversely affect pulp vitality.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Cárie Dentária/terapia
Preparo da Cavidade Dentária/métodos
Dentina/microbiologia
Lasers de Estado Sólido/uso terapêutico
[Mh] Termos MeSH secundário: Cárie Dentária/diagnóstico
Polpa Dentária/fisiologia
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Positivas/isolamento & purificação
Seres Humanos
Raios Infravermelhos
Reação em Cadeia da Polimerase em Tempo Real
Temperatura Ambiente
Termografia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


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[PMID]:29317632
[Au] Autor:Fang G; Li W; Shen X; Perez-Aguilar JM; Chong Y; Gao X; Chai Z; Chen C; Ge C; Zhou R
[Ad] Endereço:School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.
[Ti] Título:Differential Pd-nanocrystal facets demonstrate distinct antibacterial activity against Gram-positive and Gram-negative bacteria.
[So] Source:Nat Commun;9(1):129, 2018 01 09.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Noble metal-based nanomaterials have shown promise as potential enzyme mimetics, but the facet effect and underlying molecular mechanisms are largely unknown. Herein, with a combined experimental and theoretical approach, we unveil that palladium (Pd) nanocrystals exhibit facet-dependent oxidase and peroxidase-like activities that endow them with excellent antibacterial properties via generation of reactive oxygen species. The antibacterial efficiency of Pd nanocrystals against Gram-positive bacteria is consistent with the extent of their enzyme-like activity, that is {100}-faceted Pd cubes with higher activities kill bacteria more effectively than {111}-faceted Pd octahedrons. Surprisingly, a reverse trend of antibacterial activity is observed against Gram-negative bacteria, with Pd octahedrons displaying stronger penetration into bacterial membranes than Pd nanocubes, thereby exerting higher antibacterial activity than the latter. Our findings provide a deeper understanding of facet-dependent enzyme-like activities and might advance the development of noble metal-based nanomaterials with both enhanced and targeted antibacterial activities.
[Mh] Termos MeSH primário: Antibacterianos/química
Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Positivas/crescimento & desenvolvimento
Nanopartículas/química
Paládio/química
[Mh] Termos MeSH secundário: Antibacterianos/metabolismo
Antibacterianos/farmacologia
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Microscopia Eletrônica de Transmissão
Nanopartículas/metabolismo
Nanopartículas/ultraestrutura
Oxirredutases/metabolismo
Paládio/metabolismo
Paládio/farmacologia
Peroxidase/metabolismo
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Reactive Oxygen Species); 5TWQ1V240M (Palladium); EC 1.- (Oxidoreductases); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02502-3


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[PMID]:29254300
[Au] Autor:Khalid M; Bilal M; Hassani D; Iqbal HMN; Huang D
[Ad] Endereço:School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
[Ti] Título:Antimicrobial, antioxidant, cytotoxicity and LC-MS analyses of Aerva javanica: an ethnomedicinally important plant.
[So] Source:J Biol Regul Homeost Agents;31(4):963-969, 2017 Oct-Dec.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:In this study, Aerva javanica was used to extract the essential oil with notable medicinal activities. The chemical composition was investigated by high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC-MS). Ten major chemical compounds were identified as flavonoids derivatives, dihydroxylated and glycosylated metabolites. The antimicrobial, antioxidant, and cytotoxicity activities were tested using agar well-diffusion assay, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging and linoleic acid oxidation assays and hemolytic assay against human erythrocytes (RBCs), respectively. Plant extracts exhibited different extents of antimicrobial activities against selected bacterial and fungal strains; however, the essential oil displayed potent antimicrobial activity against all the tested strains. The percentage inhibition of linoleic acid oxidation and inhibitory concentration (IC50) were recorded to be in the range of 42.45-96.21% and 14.21-38.18 µg/mL, respectively. Cytotoxicity profile of A. javanica extracts and essential oil was found in the range of 5.82 to 14.47%. In conclusion, A. javanica essential oil could be a potential alternative to chemical additives in the food and pharmaceutical industries.
[Mh] Termos MeSH primário: Amaranthaceae/química
Anti-Infecciosos/farmacologia
Antioxidantes/farmacologia
Citotoxinas/farmacologia
Flavonoides/farmacologia
Óleos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Anti-Infecciosos/isolamento & purificação
Antioxidantes/isolamento & purificação
Compostos de Bifenilo/antagonistas & inibidores
Compostos de Bifenilo/química
Citotoxinas/isolamento & purificação
Flavonoides/isolamento & purificação
Fungos/efeitos dos fármacos
Fungos/crescimento & desenvolvimento
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Positivas/efeitos dos fármacos
Bactérias Gram-Positivas/crescimento & desenvolvimento
Concentração Inibidora 50
Ácido Linoleico/química
Testes de Sensibilidade Microbiana
Óleos Voláteis/isolamento & purificação
Oxirredução
Picratos/antagonistas & inibidores
Picratos/química
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Cytotoxins); 0 (Flavonoids); 0 (Oils, Volatile); 0 (Picrates); 0 (Plant Extracts); 9KJL21T0QJ (Linoleic Acid); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


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[PMID]:29232572
[Au] Autor:Pandiyan N; Murugesan B; Sonamuthu J; Samayanan S; Mahalingam S
[Ad] Endereço:Advanced Green Chemistry Lab, Department of Industrial Chemistry, School of Chemical Sciences, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
[Ti] Título:Facile biological synthetic strategy to morphologically aligned CeO /ZrO core nanoparticles using Justicia adhatoda extract and ionic liquid: Enhancement of its bio-medical properties.
[So] Source:J Photochem Photobiol B;178:481-488, 2018 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:In this study, a typical green synthesis route has approached for CeO /ZrO core metal oxide nanoparticles using ionic liquid mediated Justicia adhatoda extract. This synthesis method is carried out at simple room temperature condition to obtain the core metal oxide nanoparticles. XRD, SEM and TEM studies employed to study the crystalline and surface morphological properties under nucleation, growth, and aggregation processes. CeO /ZrO core metal oxides display agglomerated nano stick-like structure with 20-45nm size. GC-MS spectroscopy confirms the presence of vasicinone and N,N-Dimethylglycine present in the plant extract, which are capable of converting the corresponding metal ion precursor to CeO /ZrO core metal oxide nanoparticles. In FTIR, the corresponding stretching for Ce-O and Zr-O bands indicated at 498 and 416cm and Raman spectroscopy also supports typical stretching frequencies at 463 and 160cm . Band gap energy of the CeO /ZrO core metal oxide is 3.37eV calculated from UV- DRS spectroscopy. The anti-bacterial studies performed against a set of bacterial strains the result showed that core metal oxide nanoparticles more susceptible to gram-positive (G+) bacteria than gram-negative (G-) bacteria. A unique feature of the antioxidant behaviors core metal oxides reduces the concentration of DPPH radical up to 89%. The CeO /ZrO core metal oxide nanoparticles control the S. marcescent bio-film formation and restrict the quorum sensing. The toxicology behavior of CeO /ZrO core metal oxide NPs is found due to the high oxygen site vacancies, ROS formation, smallest particle size and higher surface area. This type of green synthesis route may efficient and the core metal oxide nanoparticles will possess a good bio-medical agent in future.
[Mh] Termos MeSH primário: Antibacterianos/química
Cério/química
Líquidos Iônicos/química
Justicia/química
Nanopartículas Metálicas/química
Zircônio/química
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Antioxidantes/química
Biofilmes/efeitos dos fármacos
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Química Verde
Justicia/metabolismo
Nanopartículas Metálicas/toxicidade
Microscopia Eletrônica de Varredura
Tamanho da Partícula
Extratos Vegetais/química
Folhas de Planta/química
Folhas de Planta/metabolismo
Percepção de Quorum/efeitos dos fármacos
Serratia marcescens/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antioxidants); 0 (Ionic Liquids); 0 (Plant Extracts); 30K4522N6T (Cerium); 619G5K328Y (ceric oxide); C6V6S92N3C (Zirconium); S38N85C5G0 (zirconium oxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


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[PMID]:29195216
[Au] Autor:Popov AL; Zholobak NM; Balko OI; Balko OB; Shcherbakov AB; Popova NR; Ivanova OS; Baranchikov AE; Ivanov VK
[Ad] Endereço:Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region 142290, Russia.
[Ti] Título:Photo-induced toxicity of tungsten oxide photochromic nanoparticles.
[So] Source:J Photochem Photobiol B;178:395-403, 2018 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:We synthesised a new type of photochromic tungsten oxide nanoparticles, analysed their photocatalytic activity and carried out a thorough analysis of their effect on prokaryotic and eukaryotic organisms. Ultrasmall hydrated tungsten oxide nanoparticles were prepared by means of hydrothermal treatment of tungstic acid in the presence of polyvinylpyrrolidone as a template, stabiliser and growth regulator. Tungstic acid was synthesised through an ion-exchange method using sodium tungstate solution and a strongly acidic cation exchange resin. Upon illumination, photochromic nanoparticles of WO were shown to increase greatly their toxicity against both bacterial (both gram-positive and gram-negative - P. aeruginosa, E. coli and S. aureus) and mammalian cells (primary mouse embryonic fibroblasts); under the same conditions, fungi (C. albicans) were less sensitive to the action of tungsten oxide nanoparticles. UV irradiation of primary mouse fibroblasts in the presence of WO nanoparticles demonstrated a time- and dose-dependent toxic effect, the latter leading to a significant decrease in dehydrogenase activity and an increase in the number of dead cells. WO nanoparticles were photocatalytically active under both UV light and even diffused daylight filtered through a window glass, leading to indigo carmine organic dye discolouration. The obtained experimental data not only show good prospects for biomedical applications of tungsten trioxide, but also demonstrate the need for clear control of biosafety when it is used in various household materials and appliances.
[Mh] Termos MeSH primário: Nanopartículas Metálicas/química
Óxidos/química
Tungstênio/química
[Mh] Termos MeSH secundário: Animais
Catálise
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos da radiação
Corantes/química
Fungos/efeitos dos fármacos
Fungos/efeitos da radiação
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/efeitos da radiação
Bactérias Gram-Positivas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos da radiação
Nanopartículas Metálicas/toxicidade
Camundongos
Microscopia de Fluorescência
Oxirredutases/metabolismo
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Oxides); 940E10M08M (tungsten oxide); EC 1.- (Oxidoreductases); V9306CXO6G (Tungsten)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE


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[PMID]:29298353
[Au] Autor:Nehme H; Saulnier P; Ramadan AA; Cassisa V; Guillet C; Eveillard M; Umerska A
[Ad] Endereço:MINT, UNIV Angers, INSERM 1066, CNRS 6021, Université Bretagne Loire, Angers, France.
[Ti] Título:Antibacterial activity of antipsychotic agents, their association with lipid nanocapsules and its impact on the properties of the nanocarriers and on antibacterial activity.
[So] Source:PLoS One;13(1):e0189950, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial antibiotic resistance is an emerging public health problem worldwide; therefore, new therapeutic strategies are needed. Many studies have described antipsychotic compounds that present antibacterial activity. Hence, the aims of this study were to evaluate the in vitro antibacterial activity of antipsychotics belonging to different chemical families, to assess the influence of their association with lipid nanocapsules (LNCs) on their antimicrobial activity as well as drug release and to study the uptake of LNCs by bacterial cells. Antibacterial activity was evaluated against Gram-positive Staphylococcus aureus and Gram negative Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii by minimum inhibitory concentration (MIC) assay, and the capability of killing tested microorganisms was evaluated by time kill assay. LNCs were prepared by phase inversion method, and the antipsychotic agents were incorporated using pre-loading and post-loading strategies. Only phenothiazines and thioxanthenes showed antibacterial activity, which was independent of antibiotic-resistance patterns. Loading the nanocarriers with the drugs affected the properties of the former, particularly their zeta potential. The release rate depended on the drug and its concentration-a maximum of released drug of less than 40% over 24 hours was observed for promazine. The influence of the drug associations on the antibacterial properties was concentration-dependent since, at low concentrations (high nanocarrier/drug ratio), the activity was lost, probably due to the high affinity of the drug to nanocarriers and slow release rate, whereas at higher concentrations, the activity was well maintained for the majority of the drugs. Chlorpromazine and thioridazine increased the uptake of the LNCs by bacteria compared with blank LNCs, even below the minimum inhibitory concentration.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antipsicóticos/farmacologia
Lipídeos/química
Nanocápsulas/química
[Mh] Termos MeSH secundário: Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Técnicas In Vitro
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antipsychotic Agents); 0 (Lipids); 0 (Nanocapsules)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189950


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[PMID]:29293083
[Au] Autor:Li X; Qi X; Yuan G; Ju S; Yu Z; Deng W; Liu Y; Li Y; Bu X; Ding M; Li Q; Guo X
[Ad] Endereço:1​Department of Anti-Infection, The Institute of Clinical Pharmacology, Peking University First Hospital, Beijing, PR China.
[Ti] Título:Microbiological profile and clinical characteristics of diabetic foot infection in northern China: a retrospective multicentre survey in the Beijing area.
[So] Source:J Med Microbiol;67(2):160-168, 2018 Feb.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: We aimed to define the microbiological characteristics of diabetic foot infection in patients in the Beijing area and to explore the demographic and clinical factors correlated with pathogen distribution. METHODOLOGY: As part of a retrospective multicentre surveillance program conducted in eight hospitals in Beijing 2010-2014, we recruited all inpatients for whom bacterial culture had been performed. Demographic, clinical, laboratory and surgery data were obtained from medical records. Statistical analysis was performed to analyse data on microbiological and clinical characteristics.Results/Key findings. A total of 456 cases were included. The culture positivity was 95.4 %. Among all patients with positive cultures, 88 cases (20.2 %) had polymicrobial infections. Five hundred and fifty-one species were isolated from all specimens, including 39.6 % Gram-positive bacteria and 57.5 % Gram-negative bacteria. Enterobacteriaceae accounted for 41.0 % of all isolates. Staphylococcus aureus (17.1 %), Pseudomonas aeruginosa (13.1 %), Proteus spp. (9.8 %), Escherichia coli (9.3 %) and coagulase-negative Staphylococcus (8.3 %) were the most frequently isolated species. The rate of resistance to methicillin was 24.5 % for S. aureus. The susceptibility of P. aeruginosa to all antibiotics was over 60 %. The rate of extended-spectrum ß-lactamase production among E. coli was 52.6 %. P. aeruginosa and Enterobacteriaceae show high sensitivity to piperacillin/tazobactam, carbapenems and amikacin. Multivariate analysis showed that patient age >60 years was independently associated with Gram-negative rods. CONCLUSIONS: Enterobacteriaceae were the most frequently isolated organisms in our area. Older patients were more likely to suffer from Gram-negative rod infections. Gram-negative rods show high sensitivity to piperacillin/tazobactam, carbapenems and amikacin.
[Mh] Termos MeSH primário: Infecções Bacterianas/microbiologia
Pé Diabético/microbiologia
Enterobacteriaceae/isolamento & purificação
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Positivas/isolamento & purificação
[Mh] Termos MeSH secundário: Idoso
Antibacterianos/farmacologia
Antibacterianos/uso terapêutico
Carbapenêmicos/farmacologia
Carbapenêmicos/uso terapêutico
China
Pé Diabético/diagnóstico
Pé Diabético/tratamento farmacológico
Enterobacteriaceae/classificação
Enterobacteriaceae/efeitos dos fármacos
Feminino
Bactérias Gram-Negativas/classificação
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/classificação
Bactérias Gram-Positivas/efeitos dos fármacos
Seres Humanos
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Estudos Retrospectivos
Fatores de Risco
Inquéritos e Questionários
Centros de Atenção Terciária
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Carbapenems)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000658


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[PMID]:29025667
[Au] Autor:Alvarado-Gomez E; Perez-Diaz M; Valdez-Perez D; Ruiz-Garcia J; Magaña-Aquino M; Martinez-Castañon G; Martinez-Gutierrez F
[Ad] Endereço:Microbiology Laboratory, Facultad de Ciencias Quimicas, Universidad Autonoma de San Luis Potosi, Av. Manuel Nava No. 6, CP 78210 San Luis Potosi, S.L.P., Mexico.
[Ti] Título:Adhesion forces of biofilms developed in vitro from clinical strains of skin wounds.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:336-344, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A biofilm is a very complex consortium formed by a mix of different microorganisms, which have become an important health problem, because its formation is a resistance mechanism used by bacteria against antibiotics or the immune system. In this work, we show differences between some physicochemical properties of biofilms in mono- and multi-species, formed by bacteria from clinical samples of infected chronic wounds. Of the most prevalent bacteria in wounds, two mono- and one multi-species biofilms were developed in vitro by Drip Flow Reactor: one biofilm was developed by S. aureus, other by P. aeruginosa, and a third one by the mix of both strains. With these biofilms, we determined microbial growth by plate counting, and their physicochemical characterization by Atomic Force Microscopy, Raman Micro-Spectroscopy and Scanning Electron Microscopy. We found that the viability of S. aureus was less than P. aeruginosa in multi-species biofilm. However, the adhesion force of S. aureus is much higher than that of P. aeruginosa, but it decreased while that of P. aeruginosa increased in the multi-species biofilm. In addition, we found free pyrimidines functional groups in the P. aeruginosa biofilm and its mix with S. aureus. Surprisingly, each bacterium alone formed single layer biofilms, while the mix bacteria formed a multilayer biofilm at the same observation time. Our results show the necessity to evaluate biofilms from clinically isolated strains and have a better understanding of the adhesion forces of bacteria in biofilm multispecies, which could be of prime importance in developing more effective treatments against biofilm formation.
[Mh] Termos MeSH primário: Aderência Bacteriana/fisiologia
Biofilmes/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Negativas/fisiologia
Bactérias Gram-Positivas/crescimento & desenvolvimento
Bactérias Gram-Positivas/isolamento & purificação
Bactérias Gram-Positivas/fisiologia
Seres Humanos
Microscopia de Força Atômica
Microscopia Eletrônica de Varredura
Dermatopatias/microbiologia
Dermatopatias/patologia
Análise Espectral Raman
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE



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