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Pesquisa : B03.510.024.049.050 [Categoria DeCS]
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[PMID]:29216246
[Au] Autor:Yassin AF; Langenberg S; Huntemann M; Clum A; Pillay M; Palaniappan K; Varghese N; Mikhailova N; Mukherjee S; Reddy TBK; Daum C; Shapiro N; Ivanova N; Woyke T; Kyrpides NC
[Ad] Endereço:Institut für medizinische Mikrobiologie und Immunologie der Universität Bonn, Bonn, Germany.
[Ti] Título:Draft genome sequence of Actinotignum schaalii DSM 15541T: Genetic insights into the lifestyle, cell fitness and virulence.
[So] Source:PLoS One;12(12):e0188914, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The permanent draft genome sequence of Actinotignum schaalii DSM 15541T is presented. The annotated genome includes 2,130,987 bp, with 1777 protein-coding and 58 rRNA-coding genes. Genome sequence analysis revealed absence of genes encoding for: components of the PTS systems, enzymes of the TCA cycle, glyoxylate shunt and gluconeogensis. Genomic data revealed that A. schaalii is able to oxidize carbohydrates via glycolysis, the nonoxidative pentose phosphate and the Entner-Doudoroff pathways. Besides, the genome harbors genes encoding for enzymes involved in the conversion of pyruvate to lactate, acetate and ethanol, which are found to be the end products of carbohydrate fermentation. The genome contained the gene encoding Type I fatty acid synthase required for de novo FAS biosynthesis. The plsY and plsX genes encoding the acyltransferases necessary for phosphatidic acid biosynthesis were absent from the genome. The genome harbors genes encoding enzymes responsible for isoprene biosynthesis via the mevalonate (MVA) pathway. Genes encoding enzymes that confer resistance to reactive oxygen species (ROS) were identified. In addition, A. schaalii harbors genes that protect the genome against viral infections. These include restriction-modification (RM) systems, type II toxin-antitoxin (TA), CRISPR-Cas and abortive infection system. A. schaalii genome also encodes several virulence factors that contribute to adhesion and internalization of this pathogen such as the tad genes encoding proteins required for pili assembly, the nanI gene encoding exo-alpha-sialidase, genes encoding heat shock proteins and genes encoding type VII secretion system. These features are consistent with anaerobic and pathogenic lifestyles. Finally, resistance to ciprofloxacin occurs by mutation in chromosomal genes that encode the subunits of DNA-gyrase (GyrA) and topisomerase IV (ParC) enzymes, while resistant to metronidazole was due to the frxA gene, which encodes NADPH-flavin oxidoreductase.
[Mh] Termos MeSH primário: Actinomycetaceae/genética
Genoma Bacteriano
[Mh] Termos MeSH secundário: Actinomycetaceae/classificação
Actinomycetaceae/metabolismo
Actinomycetaceae/patogenicidade
Antibacterianos/farmacologia
Metabolismo dos Carboidratos
Resistência Microbiana a Medicamentos
Fermentação
Genótipo
Metabolismo dos Lipídeos
Estresse Oxidativo
Fenótipo
Filogenia
Ácido Pirúvico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 8558G7RUTR (Pyruvic Acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188914


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[PMID]:28257873
[Au] Autor:Karstrup CC; Pedersen HG; Jensen TK; Agerholm JS
[Ad] Endereço:Department of Large Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 68, DK-1870 Frederiksberg, Denmark.
[Ti] Título:Bacterial invasion of the uterus and oviducts in bovine pyometra.
[So] Source:Theriogenology;93:93-98, 2017 Apr 15.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pyometra is a common disease of cattle that causes infertility and thereby financial losses to the cattle industry. Bacteria involved in the development and progression of pyometra have been investigated by microbial culture but their tissue invading abilities, which is an important aspect of bacterial pathogenicity and development of lesions, have not been investigated. Bacterial invasion of the uterus and oviducts was studied in 21 cows diagnosed with pyometra at the time of slaughter by applying fluorescence in situ hybridization using probes targeting 16S ribosomal RNA of Fusobacterium necrophorum, Porphyromonas levii, Trueperella pyogenes and the overall bacterial domain Bacteria. Fusobacterium necrophorum and P. levii were found to invade the endometrium, especially if the endometrium was ulcerated, and penetrated deep into the lamina propria. These species co-localized within the tissue thus indicating a synergism. Trueperella pyogenes did not invade the uterine tissue. In addition to endometrial lesions, most cows with pyometra also had salpingitis but without significant bacterial invasion of the oviductal wall.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Doenças dos Bovinos/microbiologia
Tubas Uterinas/microbiologia
Piometra/veterinária
Útero/microbiologia
[Mh] Termos MeSH secundário: Actinomycetaceae/genética
Actinomycetaceae/isolamento & purificação
Animais
Bactérias/genética
Bovinos
Endométrio/microbiologia
Feminino
Fusobacterium necrophorum/genética
Fusobacterium necrophorum/isolamento & purificação
Hibridização in Situ Fluorescente/veterinária
Porphyromonas/genética
Porphyromonas/isolamento & purificação
Piometra/microbiologia
RNA Ribossômico 16S/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE


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[PMID]:28246411
[Au] Autor:Paetsch C; Fenton K; Perrett T; Janzen E; Clark T; Shearer J; Jelinski M
[Ad] Endereço:Paetsch Veterinary Services, Muenster, Saskatchewan (Paetsch); Feedlot Health Management Services, Oktotoks, Alberta (Fenton, Perrett); Production Animal Health, University of Calgary Faculty of Veterinary Medicine, Calgary, Alberta (Janzen, Clark); Veterinary Diagnostic and Production Animal Medici
[Ti] Título:Prospective case-control study of toe tip necrosis syndrome (TTNS) in western Canadian feedlot cattle.
[So] Source:Can Vet J;58(3):247-254, 2017 Mar.
[Is] ISSN:0008-5286
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:A case-control study was conducted to investigate potential risk factors for toe tip necrosis syndrome (TTNS) in western Canadian feedlot cattle. Feedlot veterinarians provided hooves from 222 animals that died of either TTNS ("cases") or from all other causes ("controls"). The claws were sectioned by researchers to confirm the diagnoses; there was very good agreement between the practitioners' field diagnosis and that of the researchers (Cohen's kappa = 0.81; < 0.001). The sole thickness of the apical white line region was thinner ( < 0.001) in the cases (3.74 mm) than the controls (4.72 mm). Claws from cases were 5.0 [95% confidence interval (CI): 1.5 to 8.6; < 0.001] and 7.3 times (95% CI: 1.5 to 69.3; < 0.01) more likely than those of controls to yield a heavy growth of and respectively. Cases were 4.4 times (95% CI: 4.4 to 22.9; < 0.001) more likely to be acutely/transiently infected with bovine viral diarrhea virus than were controls. The findings support the hypothesis that TTNS is initiated by excessive wear along the white line, leading to separation and bacterial colonization of the 3rd phalangeal bone (P3) and associated soft tissues.
[Mh] Termos MeSH primário: Doenças dos Bovinos/epidemiologia
Doenças do Pé/veterinária
Casco e Garras/patologia
Coxeadura Animal/epidemiologia
[Mh] Termos MeSH secundário: Actinomycetaceae
Alberta/epidemiologia
Animais
Estudos de Casos e Controles
Bovinos
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/virologia
Vírus da Diarreia Viral Bovina
Escherichia coli
Doenças do Pé/epidemiologia
Doenças do Pé/microbiologia
Doenças do Pé/virologia
Casco e Garras/microbiologia
Casco e Garras/virologia
Coxeadura Animal/microbiologia
Coxeadura Animal/virologia
Necrose
Estudos Prospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE


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[PMID]:28188901
[Au] Autor:Zhang D; Zhao J; Wang Q; Liu Y; Tian C; Zhao Y; Yu L; Liu M
[Ad] Endereço:Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
[Ti] Título:Trueperella pyogenes isolated from dairy cows with endometritis in Inner Mongolia, China: Tetracycline susceptibility and tetracycline-resistance gene distribution.
[So] Source:Microb Pathog;105:51-56, 2017 Apr.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Trueperella pyogenes plays a crucial role in endometritis pathogenesis and is also associated with many infections, including metritis, mastitis, arthritis and liver abscessation, in many domestic animals. In this study, we investigated the prevalence of tetracycline resistance in T. pyogenes isolated from dairy cows with endometritis in Inner Mongolia, China, and we assessed tetracycline-resistance gene distribution among the isolates. Our results indicated that 68.7% and 62.5% of the isolates were resistant to tetracycline and doxycycline, respectively, and the rate of resistance to metacycline was 18.8%. The tetracycline resistance gene tetK was present in all isolates (n = 32), whereas the tetM gene was identified in 12.5% and 9.4% of the isolates, in the chromosome and plasmid, respectively. Strains carrying tetW were also common in the chromosome and plasmid, with abundances of 53.1% and 46.9%, respectively. However, tetO and otrA were absent in all isolates. The resistance phenotype analysis indicated that 6.3% of strains were susceptible to all tetracyclines, while 3.1% showed resistance to all tetracyclines.
[Mh] Termos MeSH primário: Actinomycetaceae/isolamento & purificação
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/microbiologia
Endometrite/veterinária
Resistência a Tetraciclina
[Mh] Termos MeSH secundário: Actinomycetaceae/efeitos dos fármacos
Actinomycetaceae/genética
Animais
Antibacterianos/farmacologia
Bovinos
China/epidemiologia
Cromossomos Bacterianos
Endometrite/epidemiologia
Endometrite/microbiologia
Feminino
Genes Bacterianos
Plasmídeos
Prevalência
Tetraciclinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Tetracyclines)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170212
[St] Status:MEDLINE


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[PMID]:28144921
[Au] Autor:Zhao K; Li W; Huang T; Song X; Zhang X; Yue B
[Ad] Endereço:Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, 610064, Chengdu, People's Republic of China.
[Ti] Título:Comparative transcriptome analysis of Trueperella pyogenes reveals a novel antimicrobial strategy.
[So] Source:Arch Microbiol;199(5):649-655, 2017 Jul.
[Is] ISSN:1432-072X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Trueperella pyogenes is a prevalent opportunistic bacterium that normally causes diverse suppurative lesions, endometritis and pneumonia in various economically important animals. Although the genomic information of this species has been announced, little is known about its functional profiles. In this study, by performing a comparative transcriptome analysis between the highly and moderately virulent T. pyogenes isolates, we found the expression of a LuxR-type DNA-binding response regulator, PloR, was significantly up-regulated in the highly virulent T. pyogenes. Protein crystal structure prediction and primary functional assessment suggested that, the quorum-sensing signal molecules of Gram-negative bacteria such as Pseudomonas aeruginosa and Escherichia coli could significantly inhibit the growth, biofilm production and hemolysis of T. pyogenes by binding to the upstream sensor histidine kinase, PloS. Therefore, the PloS/PlosR two-component regulatory system might dominate the virulence of T. pyogenes. Our findings provide a major advance in understanding the pathogenesis of T. pyogenes, and may shed new light on the development of novel therapeutic strategies to control T. pyogenes infection.
[Mh] Termos MeSH primário: Actinomycetaceae/genética
Actinomycetaceae/patogenicidade
Infecções por Actinomycetales/patologia
Proteínas de Ligação a DNA/biossíntese
Perfilação da Expressão Gênica
Histidina Quinase/metabolismo
[Mh] Termos MeSH secundário: Actinomycetaceae/crescimento & desenvolvimento
Infecções por Actinomycetales/microbiologia
Animais
Anti-Infecciosos
Biofilmes/crescimento & desenvolvimento
Escherichia coli/metabolismo
Feminino
Histidina Quinase/genética
Pseudomonas aeruginosa/metabolismo
Percepção de Quorum
Virulência/genética
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (DNA-Binding Proteins); 0 (Virulence Factors); EC 2.7.13.1 (Histidine Kinase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170202
[St] Status:MEDLINE
[do] DOI:10.1007/s00203-017-1338-5


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[PMID]:27957598
[Au] Autor:Pedersen H; Senneby E; Rasmussen M
[Ad] Endereço:Department of Clinical Sciences, Division of Infection Medicine, Lund University, BMC, B14, Tornavägen 10, 22184, Lund, Sweden.
[Ti] Título:Clinical and microbiological features of Actinotignum bacteremia: a retrospective observational study of 57 cases.
[So] Source:Eur J Clin Microbiol Infect Dis;36(5):791-796, 2017 May.
[Is] ISSN:1435-4373
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The purpose of this study was to investigate the incidence, clinical presentation, and prognosis of Actinotignum bacteremia in southern Sweden. Actinotignum isolates in blood cultures were identified retrospectively between 1st January 2012 and 31st March 2016 through searches in the clinical microbiology laboratory database. The population covered by this laboratory is approximately 1.3 million. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used for species determination. Etests were used for minimum inhibitory concentration (MIC) determination. The patients' medical charts were reviewed. Fifty-eight episodes in fifty-seven patients with Actinotignum bacteremia were identified (A. schaalii = 53, A. sanguinis = 1, A. urinale = 2, and Actinotignum species = 3), which corresponds to an incidence of 11 cases per million inhabitants. Fifty-one percent of the isolates were in pure culture. The MICs were low for ß-lactam antibiotics, whereas high MICs were recorded for ciprofloxacin and trimethoprim. Patients had a median age of 82 years, 72% were male, and a majority had underlying urological conditions. Thirty-six of the patients were diagnosed with a focus from the urinary tract. Thirty-one patients developed severe sepsis and nine patients died during the hospital stay. Our study is the largest of Actinotignum bacteremia and demonstrates that it is a condition with a significant fatality that affects elderly persons with underlying conditions. ß-Lactams represent a rational treatment option.
[Mh] Termos MeSH primário: Actinomycetaceae/isolamento & purificação
Infecções por Actinomycetales/epidemiologia
Infecções por Actinomycetales/patologia
Bacteriemia/epidemiologia
Bacteriemia/patologia
[Mh] Termos MeSH secundário: Actinomycetaceae/classificação
Actinomycetaceae/efeitos dos fármacos
Infecções por Actinomycetales/microbiologia
Idoso
Idoso de 80 Anos ou mais
Antibacterianos/farmacologia
Bacteriemia/microbiologia
Sangue/microbiologia
Feminino
Seres Humanos
Incidência
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Estudos Retrospectivos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Análise de Sobrevida
Suécia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE
[do] DOI:10.1007/s10096-016-2862-y


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[PMID]:27940245
[Au] Autor:Vallet A; Noël N; Bahi R; Teicher E; Quertainmont Y; Delfraissy JF; Ferlicot S; Potron A; Goujard C; Lambotte O
[Ad] Endereço:Assistance Publique Hôpitaux de Paris, Service de Médecine Interne et Immunologie Clinique, Hôpitaux Universitaires Paris Sud, Le Kremlin Bicêtre, France.
[Ti] Título:Recurrent obstructive acute pyelonephritis: A rare form of Actinotignum (Actinobaculum) schaalii infection in a HIV-1 infected patient.
[So] Source:Anaerobe;43:75-77, 2017 Feb.
[Is] ISSN:1095-8274
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Actinobaculum schaalii is a rarely reported, anaerobic, Gram-positive bacterium which role as uropathogen is emerging. We report here the case of a 47 year old HIV-1 infected woman presented with five recurrent episodes of obstructive pyelonephritis in the context of multiple renal stones. No bacteria was found until the fifth episode, during which prolonged urinary cultures as well as 16S rDNA sequencing allowed the diagnosis of A. schaalii infection. She had developed a life-threatening condition with severe renal failure. A right nephrectomy was performed and found that the intrarenal stones were attributed to the antiretroviral therapy. The renal parenchyma corresponded to an end-stage renal disease with chronic pyelonephritis without abcesses or granules. The situation improved after six months of amoxicillin therapy.
[Mh] Termos MeSH primário: Actinomycetaceae/isolamento & purificação
Infecções por Actinomycetales/diagnóstico por imagem
Antibacterianos/uso terapêutico
Infecções por HIV/complicações
Hepatite C/complicações
Pielonefrite/diagnóstico por imagem
[Mh] Termos MeSH secundário: Actinomycetaceae/genética
Infecções por Actinomycetales/complicações
Infecções por Actinomycetales/tratamento farmacológico
Infecções por Actinomycetales/cirurgia
Amoxicilina/uso terapêutico
Coinfecção
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Feminino
Seres Humanos
Rim/patologia
Cálculos Renais
Meia-Idade
Pielonefrite/complicações
Pielonefrite/tratamento farmacológico
Pielonefrite/cirurgia
RNA Ribossômico 16S/genética
Recidiva
Análise de Sequência de DNA
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 804826J2HU (Amoxicillin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


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[PMID]:27913316
[Au] Autor:Maraki S; Evangelou G; Stafylaki D; Scoulica E
[Ad] Endereço:Department of Clinical Microbiology, Parasitology, Zoonoses and Geographical Medicine, University Hospital of Heraklion, Crete, Greece. Electronic address: sofiamaraki@in.gr.
[Ti] Título:Actinotignum schaalii subcutaneous abscesses in a patient with hidradenitis suppurativa: Case report and literature review.
[So] Source:Anaerobe;43:43-46, 2017 Feb.
[Is] ISSN:1095-8274
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Actinotignum schaalii (formerly Actinobaculum schaalii) is a Gram-positive, facultative anaerobic rod that is typically involved in urinary tract infections in elderly patients or those with underlying urological pathologies. In contrast, abscess formation caused by A. schaalii is very rare. We present a case of multiple abscesses in the perineal area in a young patient with hidradenitis suppurativa associated with A. schaalii and Prevotella melaninogenica and review the relevant literature on the topic.
[Mh] Termos MeSH primário: Abscesso/diagnóstico
Actinomycetaceae/isolamento & purificação
Infecções Bacterianas/diagnóstico
Infecções por Bacteroidaceae/complicações
Hidradenite Supurativa/complicações
Prevotella melaninogenica/isolamento & purificação
[Mh] Termos MeSH secundário: Abscesso/complicações
Abscesso/tratamento farmacológico
Actinomycetaceae/classificação
Actinomycetaceae/genética
Adulto
Infecções Bacterianas/microbiologia
Infecções por Bacteroidaceae/microbiologia
DNA Ribossômico/química
DNA Ribossômico/genética
Hidradenite Supurativa/microbiologia
Seres Humanos
Masculino
Testes de Sensibilidade Microbiana
Filogenia
Prevotella melaninogenica/classificação
Prevotella melaninogenica/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (DNA, Ribosomal)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161204
[St] Status:MEDLINE


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[PMID]:27894322
[Au] Autor:Jaureguiberry M; Madoz LV; Giuliodori MJ; Wagener K; Prunner I; Grunert T; Ehling-Schulz M; Drillich M; de la Sota RL
[Ad] Endereço:Cátedra y Servicio de Reproducción Animal, Facultad de Ciencias Veterinarias, Universidad de La Plata (FCV-UNLP), B1900AVW, La Plata, Argentina.
[Ti] Título:Identification of Escherichia coli and Trueperella pyogenes isolated from the uterus of dairy cows using routine bacteriological testing and Fourier transform infrared spectroscopy.
[So] Source:Acta Vet Scand;58(1):81, 2016 Nov 28.
[Is] ISSN:1751-0147
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Uterine disorders are common postpartum diseases in dairy cows. In practice, uterine treatment is often based on systemic or locally applied antimicrobials with no previous identification of pathogens. Accurate on-farm diagnostics are not available, and routine testing is time-consuming and cost intensive. An accurate method that could simplify the identification of uterine pathogenic bacteria and improve pathogen-specific treatments could be an important advance to practitioners. The objective of the present study was to evaluate whether a database built with uterine bacteria from European dairy cows could be used to identify bacteria from Argentinean cows by Fourier transformed infrared (FTIR) spectroscopy. Uterine samples from 64 multiparous dairy cows with different types of vaginal discharge (VD) were collected between 5 and 60 days postpartum, analyzed by routine bacteriological testing methods and then re-evaluated by FTIR spectroscopy (n = 27). RESULTS: FTIR spectroscopy identified Escherichia coli in 12 out of 14 samples and Trueperella pyogenes in 8 out of 10 samples. The agreement between the two methods was good with a Kappa coefficient of 0.73. In addition, the likelihood for bacterial growth of common uterine pathogens such as E. coli and T. pyogenes tended to increase with VD score. The odds for a positive result to E. coli or T. pyogenes was 1.88 times higher in cows with fetid VD than in herdmates with clear normal VD. CONCLUSIONS: We conclude that the presence of E. coli and T. pyogenes in uterine samples from Argentinean dairy cows can be detected with FTIR with the use of a database built with uterine bacteria from European dairy cows. Future studies are needed to determine if FTIR can be used as an alternative to routine bacteriological testing methods.
[Mh] Termos MeSH primário: Actinomycetaceae/isolamento & purificação
Infecções por Actinomycetales/veterinária
Criação de Animais Domésticos/métodos
Doenças dos Bovinos/microbiologia
Infecções por Escherichia coli/veterinária
Escherichia coli/isolamento & purificação
Espectroscopia de Infravermelho com Transformada de Fourier
[Mh] Termos MeSH secundário: Actinomycetaceae/classificação
Infecções por Actinomycetales/diagnóstico
Infecções por Actinomycetales/microbiologia
Animais
Argentina
Técnicas Bacteriológicas/veterinária
Bovinos
Doenças dos Bovinos/diagnóstico
Bases de Dados Factuais
Escherichia coli/classificação
Infecções por Escherichia coli/microbiologia
Feminino
Reprodutibilidade dos Testes
Doenças Uterinas/diagnóstico
Doenças Uterinas/microbiologia
Doenças Uterinas/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161130
[St] Status:MEDLINE


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Fotocópia
[PMID]:27759406
[Au] Autor:ElSalhy M; Söderling E; Honkala E; Fontana M; Flannagan S; Kokaras A; Paster BJ; Varghese A; Honkala S
[Ad] Endereço:Faculty of Dentistry, Kuwait University, Kuwait - Institute of Dentistry, University of Turku, Finland.
[Ti] Título:Salivary microbiota and caries occurrence in Mutans Streptococci-positive school children.
[So] Source:Eur J Paediatr Dent;17(3):188-192, 2016 Sep.
[Is] ISSN:1591-996X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:AIM: To compare the composition of the salivary microbiota in caries-affected vs. caries-free mutans streptococci (MS)- positive children with mixed dentition. MATERIALS AND METHODS: Twenty eight healthy, 11-12-year-old schoolchildren with high MS counts (>10⊃5 CFU/mL) were included in this study. The children were screened with the Dentocult SM Strip Mutans test (Orion Diagnostica, Espoo, Finland) and examined using the International Caries Detection and Assessment System (ICDAS). The microbial composition of the saliva was assessed using the Human Oral Microbe Identification Microarray (HOMIM). Microbial differences between caries-affected (n=18) and caries-free children (n=10) were compared by Mann-Whitney analysis. RESULTS: The microbiota of the caries-affected vs. caries-free children was rather similar. Abiotrophia defectiva and Actinomyces meyeri/A. odontolyticus were significantly higher in caries-affected than in caries-free children (p=0.006, 0.046, respectively). Shuttleworthia satelles was significantly higher in caries-free compared to caries-affected children (p=0.031). A. defectiva and A. meyeri/A. odontolyticus correlated positively with caries severity measured by ICDAS Caries Index (p = 0.494, 0.454, 0.400 respectively) while S. satelles was negatively correlated with caries severity (p= -0.489). CONCLUSIONS: Salivary A. defectiva and A. meyeri/A. odontolyticus and are associated with caries occurrence in MS-positive children with mixed dentition.
[Mh] Termos MeSH primário: Cárie Dentária/microbiologia
Saliva/microbiologia
Streptococcus mutans/isolamento & purificação
[Mh] Termos MeSH secundário: Abiotrophia/isolamento & purificação
Actinomyces/classificação
Actinomyces/isolamento & purificação
Actinomycetaceae/classificação
Actinomycetaceae/isolamento & purificação
Carga Bacteriana
Carnobacteriaceae/isolamento & purificação
Criança
Índice CPO
Dentição Mista
Gemella/isolamento & purificação
Bactérias Gram-Positivas/classificação
Bactérias Gram-Positivas/isolamento & purificação
Seres Humanos
Streptococcus/classificação
Streptococcus/isolamento & purificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE



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