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Pesquisa : B03.510.024.049.180.600 [Categoria DeCS]
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[PMID]:29246537
[Au] Autor:Gomide ACP; de Sá PG; Cavalcante ALQ; de Jesus Sousa T; Gomes LGR; Ramos RTJ; Azevedo V; Silva A; Folador ARC
[Ad] Endereço:Department of General Biology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Antônio Carlos, Belo Horizonte 31.270-901, Brazil. Electronic address: acybelle@gmail.com.
[Ti] Título:Heat shock stress: Profile of differential expression in Corynebacterium pseudotuberculosis biovar Equi.
[So] Source:Gene;645:124-130, 2018 Mar 01.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Transcriptome studies on Corynebacterium pseudotuberculosis have recently contributed to the understanding about this microorganism's survival mechanisms in various hostile conditions. The gene expression profile of the C. pseudotuberculosis strain 1002 (Ovis biovar), has revealed genes that are possible candidates responsible for its maintenance in adverse environments, such as those found in the host. In another strain of this bacterium, 258 (Equi biovar), a high temperature condition was simulated, in order to verify which genes are responsible for promoting the persistence of the bacterium in these conditions, since it tolerates temperatures higher than 40°C, despite being a mesophilic bacterium. It was possible to generate a list of genes using RNAseq technology that possibly contribute to the survival of the bacteria in this hostile environment. A total of 562 genes were considered as differentially expressed, then, after the fold-change cutoff, 113 were considered induced and 114 repressed, resulting in a total of 227 genes. Therefore, hypothetical proteins presented a fold change above 6, and genes characteristically in control for this type of stress, such as hspR, grpE, and dnaK, presented a fold change above 3. The clpB gene, a chaperone, drew attention due to presenting a fold change above 3 and located in a pathogenicity island. These genes may contribute towards efficient solutions to the effects caused by ulcerative lymphangitis in equines, thus attenuating the damage it causes to agribusiness.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Corynebacterium pseudotuberculosis/crescimento & desenvolvimento
Perfilação da Expressão Gênica/métodos
Análise de Sequência de RNA/métodos
[Mh] Termos MeSH secundário: Animais
Corynebacterium pseudotuberculosis/genética
Corynebacterium pseudotuberculosis/isolamento & purificação
Regulação Bacteriana da Expressão Gênica
Ilhas Genômicas
Cavalos/microbiologia
Temperatura Alta
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE


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[PMID]:28974474
[Au] Autor:Catarina Teodoro Castro B; Cançado de Faria R; Faria BF; Azevedo V; Lara Dos Santos L; Júnior MC; Machado CR; de Oliveira Lopes D
[Ad] Endereço:Laboratory of Molecular Biology, Federal University of São João Del-Rei (CCO), Av. Sebastião Gonçalves Coelho, 400, Divinópolis, MG 35501-296, Brazil. Electronic address: barbarateodoro@outlook.com.
[Ti] Título:UvrB protein of Corynebacterium pseudotuberculosis complements the phenotype of knockout Escherichia coli and recognizes DNA damage caused by UV radiation but not 8-oxoguanine in vitro.
[So] Source:Gene;639:34-43, 2018 Jan 10.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In prokaryotic cells, the UvrB protein plays a central role in nucleotide excision repair, which is involved in the recognition of bulky DNA lesions generated by chemical or physical agents. The present investigation aimed to characterize the uvrB gene of Corynebacterium pseudotuberculosis (CpuvrB) and evaluate its involvement in the DNA repair system of this pathogenic organism. In computational analysis, the alignment of the UvrB protein sequences of Escherichia coli, Mycobacterium tuberculosis, Bacillus caldotenax and Corynebacterium pseudotuberculosis showed high similarity and the catalytic amino acid residues and functional domains are preserved. A CpUvrB model was constructed by comparative modeling and presented structural similarity with the UvrB of E. coli. Moreover, in molecular docking analysis CpUvrB showed favorable interaction with EcUvrA and revealed a preserved ATP incorporation site. Heterologous functional complementation assays using E. coli uvrB-deficient cells exposed to UV irradiation showed that the CpUvrB protein contributed to an increased survival rate in relation to those in the absence of CpUvrB. Damaged oligonucleotides containing thymine dimer or 8-oxoguanine lesion were synthesized and incubated with CpUvrB protein, which was able to recognize and excise UV irradiation damage but not 8-oxoguanine. These results suggest that CpUvrB is involved in repairing lesions derived from UV light and encodes a protein orthologous to EcUvrB.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Corynebacterium pseudotuberculosis/genética
Dano ao DNA
Escherichia coli/genética
Guanina/análogos & derivados
Raios Ultravioleta
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/metabolismo
Clonagem Molecular
Corynebacterium pseudotuberculosis/metabolismo
Corynebacterium pseudotuberculosis/efeitos da radiação
Técnicas de Silenciamento de Genes
Guanina/metabolismo
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 5614-64-2 (8-hydroxyguanine); 5Z93L87A1R (Guanine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE


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[PMID]:28516859
[Au] Autor:Brum AA; Rezende AFS; Brilhante FS; Collares T; Begnine K; Seixas FK; Collares TV; Dellagostin OA; Azevedo V; Santos A; Portela RW; Borsuk S
[Ad] Endereço:1​Laboratório de Biotecnologia Infecto-parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, RS 96010-900, Brazil.
[Ti] Título:Recombinant esterase from Corynebacterium pseudotuberculosis in DNA and subunit recombinant vaccines partially protects mice against challenge.
[So] Source:J Med Microbiol;66(5):635-642, 2017 May.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: We tested the efficacy of the esterase encoded by cp1002_RS09720 from Corynebacteriumpseudotuberculosis in recombinant subunit and DNA caseous lymphadenitis (CLA) vaccines. This target was predicted as one of the best CLA vaccine candidates by mature epitope density analysis. METHODOLOGY: Gene cp1002_RS09720 was cloned into two different vectors (pAE for subunit vaccine and pTARGET for DNA vaccine). Four groups of 15 mice each were immunized with the recombinant esterase rCP09720 associated with aluminium hydroxide adjuvant (G1), pTARGET/cp09720 DNA vaccine (G2), a naked pTARGET (G3) or PBS as a negative control (G4). Immunization occurred in two doses intercalated by a 21 day interval. Twenty-one days after the last dose administration, animals were challenged with a virulent C. pseudotuberculosis MIC-6 strain. RESULTS: G1 showed high levels of IgG1 and IgG2a on days 21 and 42 post-immunization and a significant level of IFN-γ (P<0.05), suggesting a Th1 response. The protection levels obtained were 58.3 and 16.6 % for G1 and G2, respectively. CONCLUSION: The subunit vaccine composed of the recombinant esterase rCP09720 and Al(OH)3 is a promising antigenic formulation for use against CLA.
[Mh] Termos MeSH primário: Infecções por Corynebacterium/prevenção & controle
Corynebacterium pseudotuberculosis/enzimologia
Corynebacterium pseudotuberculosis/imunologia
Esterases/genética
Linfadenite/prevenção & controle
Vacinas de DNA/imunologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos
Animais
Infecções por Corynebacterium/imunologia
Corynebacterium pseudotuberculosis/genética
Corynebacterium pseudotuberculosis/patogenicidade
Citocinas/secreção
Esterases/administração & dosagem
Esterases/imunologia
Imunoglobulina G/sangue
Interferon gama/imunologia
Linfadenite/microbiologia
Camundongos
Camundongos Endogâmicos BALB C
Células Th1/imunologia
Vacinação
Vacinas de DNA/administração & dosagem
Vacinas Sintéticas/imunologia
Vacinas Sintéticas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Cytokines); 0 (Immunoglobulin G); 0 (Vaccines, DNA); 0 (Vaccines, Synthetic); 82115-62-6 (Interferon-gamma); EC 3.1.- (Esterases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000477


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[PMID]:28445543
[Au] Autor:Viana MVC; Figueiredo H; Ramos R; Guimarães LC; Pereira FL; Dorella FA; Selim SAK; Salaheldean M; Silva A; Wattam AR; Azevedo V
[Ad] Endereço:Departament of General Biology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
[Ti] Título:Comparative genomic analysis between Corynebacterium pseudotuberculosis strains isolated from buffalo.
[So] Source:PLoS One;12(4):e0176347, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Corynebacterium pseudotuberculosis is a Gram-positive, pleomorphic, facultative intracellular pathogen that causes Oedematous Skin Disease (OSD) in buffalo. To better understand the pathogenic mechanisms of OSD, we performed a comparative genomic analysis of 11 strains of C. pseudotuberculosis isolated from different buffalo found to be infected in Egypt during an outbreak that occurred in 2008. Sixteen previously described pathogenicity islands (PiCp) were present in all of the new buffalo strains, but one of them, PiCp12, had an insertion that contained both a corynephage and a diphtheria toxin gene, both of which may play a role in the adaptation of C. pseudotuberculosis to this new host. Synteny analysis showed variations in the site of insertion of the corynephage during the same outbreak. A gene functional comparison showed the presence of a nitrate reductase operon that included genes involved in molybdenum cofactor biosynthesis, which is necessary for a positive nitrate reductase phenotype and is a possible adaptation for intracellular survival. Genomes from the buffalo strains also had fusions in minor pilin genes in the spaA and spaD gene cluster (spaCX and spaYEF), which could suggest either an adaptation to this particular host, or mutation events in the immediate ancestor before this particular epidemic. A phylogenomic analysis confirmed a clear separation between the Ovis and Equi biovars, but also showed what appears to be a clustering by host species within the Equi strains.
[Mh] Termos MeSH primário: Hibridização Genômica Comparativa
Infecções por Corynebacterium/microbiologia
Corynebacterium pseudotuberculosis/genética
Genoma Bacteriano
Dermatopatias Bacterianas/microbiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Búfalos
Infecções por Corynebacterium/epidemiologia
Infecções por Corynebacterium/patologia
Corynebacterium pseudotuberculosis/classificação
Corynebacterium pseudotuberculosis/isolamento & purificação
Toxina Diftérica/classificação
Toxina Diftérica/genética
Surtos de Doenças
Egito/epidemiologia
Genômica
Sequenciamento de Nucleotídeos em Larga Escala
Família Multigênica
Filogenia
Análise de Sequência de DNA
Dermatopatias Bacterianas/epidemiologia
Dermatopatias Bacterianas/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Diphtheria Toxin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0176347


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[PMID]:28275091
[Au] Autor:Varela-Castro L; Lara-Vergara J; Ortega N; Salinas J; Colom-Cadena A; Lavín S; Tizzani P; Velarde R; Serrano E; Mentaberre G
[Ad] Endereço:Servei d'Ecopatologia de Fauna Salvatge (SEFaS), Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona (UAB), Bellaterra, Spain.
[Ti] Título:Endemic caseous lymphadenitis in a wild Caprinae population.
[So] Source:Vet Rec;180(16):405, 2017 Apr 22.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Between 2010 and 2013, an outbreak of caseous lymphadenitis (CLA) occurred in a captive stock of Iberian ibexes ( , Schinz 1838) maintained for conservation purposes in the National Game Reserve 'Ports de Tortosa i Beseit' (PTB), northeastern Spain. The aim of this study was to assess the CLA status in the free-ranging donor population of ibexes. An ELISA test to detect antibodies to CLA was performed in 360 hunter-harvested ibexes between 2007 and 2013. A spatial analysis and recursive partitioning approaches with regression tree models were used to explore CLA-associated risk factors. Nineteen per cent of ibexes had antibodies to CLA. Significant differences in seroprevalence were observed among game management areas but no clear trends of CLA occurrence were observed over the study period. Ibexes from areas of higher population density and living close to aggregation points displayed a higher probability of testing positive to CLA. These results suggest that CLA is endemic in the Iberian ibex population inhabiting PTB and density-dependent risk factors. To the best of our knowledge, this is the first record of CLA maintenance in a free-ranging wild Caprinae population.
[Mh] Termos MeSH primário: Animais Selvagens/microbiologia
Infecções por Corynebacterium/veterinária
Doenças Endêmicas/veterinária
Doenças das Cabras/epidemiologia
Linfadenite/veterinária
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/isolamento & purificação
Infecções por Corynebacterium/epidemiologia
Infecções por Corynebacterium/microbiologia
Corynebacterium pseudotuberculosis/imunologia
Ensaio de Imunoadsorção Enzimática/veterinária
Feminino
Doenças das Cabras/microbiologia
Cabras
Linfadenite/epidemiologia
Linfadenite/microbiologia
Masculino
Estudos Soroepidemiológicos
Espanha/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1136/vr.103925


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[PMID]:28176661
[Au] Autor:Coronado MA; Caruso IP; Oliveira VM; Contessoto VG; Leite VBP; Kawai LA; Arni RK; Eberle RJ
[Ad] Endereço:Multiuser Center for Biomolecular Innovation, Universidade Estadual Paulista (UNESP), Sao Jose do Rio Preto-SP, 15054-000. Brazil.
[Ti] Título:Cold Shock Protein A from Corynebacterium pseudotuberculosis: Role of Electrostatic Forces in the Stability of the Secondary Structure.
[So] Source:Protein Pept Lett;24(4):358-367, 2017.
[Is] ISSN:1875-5305
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The conformational stability of the Cold shock protein A (CspA) from C. pseudotuberculosis (Cp), a nucleic acid binding protein in function of pH and salt concentration was examined by using differential scanning calorimetry and CD spectroscopy in combination with computational analysis to identify the specify amino acids undergoing change. Our approach identified a sodiumbinding site in CpCspA and at pH 8.0 a significant reduction in the ß-sheet content was observed which resulted in a decrease of the protein thermal stability. The computational analyses identified His30 and His65 as the amino acids with the largest charge shifts at different pHs. His30/His65 are part of the extensive hydrogen bonding network and along with the ion-binding site are essential for the conformational stability of CspA.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Corynebacterium pseudotuberculosis/química
[Mh] Termos MeSH secundário: Corynebacterium pseudotuberculosis/metabolismo
Hidrogênio/química
Hidrogênio/metabolismo
Concentração de Íons de Hidrogênio
Modelos Moleculares
Ligação Proteica
Estabilidade Proteica
Estrutura Secundária de Proteína
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Sódio/química
Sódio/metabolismo
Eletricidade Estática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Recombinant Proteins); 0 (cold shock protein CS7.4, Bacteria); 7YNJ3PO35Z (Hydrogen); 9NEZ333N27 (Sodium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.2174/0929866524666170207153808


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[PMID]:28125655
[Au] Autor:Baraúna RA; Ramos RT; Veras AA; Pinheiro KC; Benevides LJ; Viana MV; Guimarães LC; Edman JM; Spier SJ; Azevedo V; Silva A
[Ad] Endereço:Laboratory of Genomics and Bioinformatics, Center of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará, Brazil.
[Ti] Título:Assessing the Genotypic Differences between Strains of Corynebacterium pseudotuberculosis biovar equi through Comparative Genomics.
[So] Source:PLoS One;12(1):e0170676, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Seven genomes of Corynebacterium pseudotuberculosis biovar equi were sequenced on the Ion Torrent PGM platform, generating high-quality scaffolds over 2.35 Mbp. This bacterium is the causative agent of disease known as "pigeon fever" which commonly affects horses worldwide. The pangenome of biovar equi was calculated and two phylogenomic approaches were used to identify clustering patterns within Corynebacterium genus. Furthermore, other comparative analyses were performed including the prediction of genomic islands and prophages, and SNP-based phylogeny. In the phylogenomic tree, C. pseudotuberculosis was divided into two distinct clades, one formed by nitrate non-reducing species (biovar ovis) and another formed by nitrate-reducing species (biovar equi). In the latter group, the strains isolated from California were more related to each other, while the strains CIP 52.97 and 1/06-A formed the outermost clade of the biovar equi. A total of 1,355 core genes were identified, corresponding to 42.5% of the pangenome. This pangenome has one of the smallest core genomes described in the literature, suggesting a high genetic variability of biovar equi of C. pseudotuberculosis. The analysis of the similarity between the resistance islands identified a higher proximity between the strains that caused more severe infectious conditions (infection in the internal organs). Pathogenicity islands were largely conserved between strains. Several genes that modulate the pathogenicity of C. pseudotuberculosis were described including peptidases, recombination enzymes, micoside synthesis enzymes, bacteriocins with antimicrobial activity and several others. Finally, no genotypic differences were observed between the strains that caused the three different types of infection (external abscess formation, infection with abscess formation in the internal organs, and ulcerative lymphangitis). Instead, it was noted that there is a higher phenetic correlation between strains isolated at California compared to the other strains. Additionally, high variability of resistance islands suggests gene acquisition through several events of horizontal gene transfer.
[Mh] Termos MeSH primário: Infecções por Corynebacterium/genética
Corynebacterium pseudotuberculosis/genética
Genoma Bacteriano/genética
Doenças dos Cavalos/genética
Rhodococcus equi/genética
[Mh] Termos MeSH secundário: Animais
Infecções por Corynebacterium/microbiologia
Corynebacterium pseudotuberculosis/patogenicidade
Genótipo
Sequenciamento de Nucleotídeos em Larga Escala
Doenças dos Cavalos/microbiologia
Cavalos/microbiologia
Filogenia
Polimorfismo de Nucleotídeo Único/genética
Rhodococcus equi/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170676


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[PMID]:27979735
[Au] Autor:Haas DJ; Dorneles EM; Spier SJ; Carroll SP; Edman J; Azevedo VA; Heinemann MB; Lage AP
[Ad] Endereço:Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
[Ti] Título:Molecular epidemiology of Corynebacterium pseudotuberculosis isolated from horses in California.
[So] Source:Infect Genet Evol;49:186-194, 2017 Apr.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Corynebacterium pseudotuberculosis biovar Equi is an important pathogen of horses. It is increasing in frequency in the United States, and is responsible for various clinical forms of infection, including external abscesses, internal abscesses of the abdominal or thoracic cavities, and ulcerative lymphangitis. The host/pathogen factors dictating the form or severity of infection are currently unknown. Our recent investigations have shown that genotyping C. pseudotuberculosis isolates using enterobacterial repetitive intergenic consensus (ERIC)-PCR is useful for understanding the evolutionary genetics of the species as well for molecular epidemiology studies. The aims of the present study were to assess (i) the genetic diversity of C. pseudotuberculosis strains isolated from horses in California, United States and (ii) the epidemiologic relationships among isolates. One hundred and seven C. pseudotuberculosis biovar Equi isolates from ninety-five horses, and two C. pseudotuberculosis biovar Ovis strains, C. pseudotuberculosis ATCC 19410 type strain and C. pseudotuberculosis 1002 vaccine strain, were fingerprinted using the ERIC 1+2-PCR. C. pseudotuberculosis isolated from horses showed a high genetic diversity, clustering in twenty-seven genotypes with a diversity index of 0.91. Minimal spanning tree showed four major clonal complexes with a pattern of temporal clustering. Strains isolated from the same horse showed identical ERIC 1+2-PCR genotype, with the exception of two strains isolated from the same animal that showed distinct genotypes, suggesting a co-infection. We found no strong genetic signals related to clinical form (including internal versus external infections). However, temporal clustering of genotypes was observed.
[Mh] Termos MeSH primário: Infecções por Corynebacterium/veterinária
Corynebacterium pseudotuberculosis/genética
DNA Bacteriano/genética
DNA Intergênico/genética
Doenças dos Cavalos/epidemiologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
California/epidemiologia
Análise por Conglomerados
Infecções por Corynebacterium/epidemiologia
Infecções por Corynebacterium/microbiologia
Infecções por Corynebacterium/patologia
Corynebacterium pseudotuberculosis/classificação
Corynebacterium pseudotuberculosis/isolamento & purificação
Impressões Digitais de DNA
Variação Genética
Genótipo
Doenças dos Cavalos/microbiologia
Doenças dos Cavalos/patologia
Cavalos/microbiologia
Epidemiologia Molecular
Filogenia
Índice de Gravidade de Doença
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Intergenic)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


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[PMID]:27808444
[Au] Autor:Mariutti RB; Chaves-Moreira D; Vuitika L; Caruso ÍP; Coronado MA; Azevedo VA; Murakami MT; Veiga SS; Arni RK
[Ad] Endereço:Department of Physics, Multiuser Center for Biomolecular Innovation, UNESP, São José do Rio Preto, SP, Brazil.
[Ti] Título:Bacterial and Arachnid Sphingomyelinases D: Comparison of Biophysical and Pathological Activities.
[So] Source:J Cell Biochem;118(8):2053-2063, 2017 Aug.
[Is] ISSN:1097-4644
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sphingomyelinases D have only been identified in arachnid venoms, Corynebacteria, Arcanobacterium, Photobacterium and in the fungi Aspergillus and Coccidioides. The arachnid and bacterial enzymes share very low sequence identity and do not contain the HKD sequence motif characteristic of the phospholipase D superfamily, however, molecular modeling and circular dichroism of SMases D from Loxosceles intermedia and Corynebacterium pseudotuberculosis indicate similar folds. The phospholipase, hemolytic and necrotic activities and mice vessel permeabilities were compared and both enzymes possess the ability to hydrolyze phospholipids and also promote similar pathological reactions in the host suggesting the existence of a common underlying mechanism in tissue disruption. J. Cell. Biochem. 118:2053-2063, 2017. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/toxicidade
Proteínas de Bactérias/toxicidade
Permeabilidade Capilar/efeitos dos fármacos
Corynebacterium pseudotuberculosis/química
Diester Fosfórico Hidrolases/toxicidade
Aranhas/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas de Artrópodes/genética
Proteínas de Artrópodes/metabolismo
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Clonagem Molecular
Corynebacterium pseudotuberculosis/enzimologia
Corynebacterium pseudotuberculosis/patogenicidade
Eritrócitos/efeitos dos fármacos
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Hemólise/efeitos dos fármacos
Cavalos
Seres Humanos
Camundongos
Diester Fosfórico Hidrolases/genética
Diester Fosfórico Hidrolases/metabolismo
Coelhos
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Proteínas Recombinantes/toxicidade
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Carneiro Doméstico
Pele/efeitos dos fármacos
Pele/patologia
Aranhas/enzimologia
Aranhas/patogenicidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (Bacterial Proteins); 0 (Recombinant Proteins); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 3.1.4.41 (sphingomyelin phosphodiesterase D)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161104
[St] Status:MEDLINE
[do] DOI:10.1002/jcb.25781


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[PMID]:27902437
[Au] Autor:De Zoysa A; Efstratiou A; Mann G; Harrison TG; Fry NK
[Ad] Endereço:1​Respiratory and Vaccine Preventable Bacteria Reference Unit, Public Health England, Microbiology Reference Services, National Infection Service, London, UK.
[Ti] Título:Development, validation and implementation of a quadruplex real-time PCR assay for identification of potentially toxigenic corynebacteria.
[So] Source:J Med Microbiol;65(12):1521-1527, 2016 Dec.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Toxigenic corynebacteria are uncommon in the UK; however, laboratory confirmation by the national reference laboratory can inform public health action according to national guidelines. Standard phenotypic tests for identification and toxin expression of isolates can take from ≥24 to ≥48 h from receipt. To decrease the time to result, a real-time PCR (qPCR) assay was developed for confirmation of both identification of Corynebacterium diphtheriae and Corynebacterium ulcerans/Corynebacterium pseudotuberculosis and detection of the diphtheria toxin gene. Target genes were the RNA polymerase ß-subunit-encoding gene (rpoB) and A-subunit of the diphtheria toxin gene (tox). Green fluorescent protein DNA (gfp) was used as an internal process control. qPCR results were obtained within 3 to 4 h after receipt of isolate. The assay was validated according to published guidelines and demonstrated high diagnostic sensitivity (100 %), high specificity (98-100 %) and positive and negative predictive values of 91 to 100 % and 100 %, respectively, compared to both block-based PCR and the Elek test, together with a greatly reduced time from isolate receipt to reporting. Limitations of the qPCR assay were the inability to distinguish between C. ulcerans and C. pseudotuberculosis and that the presence of the toxin gene as demonstrated by qPCR may not always predict toxin expression. Thus, confirmation of expression of diphtheria toxin is always sought using the phenotypic Elek test. The new qPCR assay was formally introduced as the front-line test for putative toxigenic corynebacteria to inform public health action in England and Wales on 1 April 2014.
[Mh] Termos MeSH primário: Infecções por Corynebacterium/diagnóstico
Infecções por Corynebacterium/microbiologia
Corynebacterium diphtheriae/isolamento & purificação
Corynebacterium pseudotuberculosis/isolamento & purificação
Corynebacterium/genética
Corynebacterium/isolamento & purificação
Difteria/diagnóstico
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Corynebacterium diphtheriae/genética
Corynebacterium diphtheriae/patogenicidade
Corynebacterium pseudotuberculosis/genética
Difteria/microbiologia
Toxina Diftérica/biossíntese
Toxina Diftérica/genética
Inglaterra
Seres Humanos
Valor Preditivo dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Diphtheria Toxin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170127
[Lr] Data última revisão:
170127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000382



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