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Pesquisa : B03.510.024.049.525.500 [Categoria DeCS]
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  1 / 12706 MEDLINE  
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[PMID]:28461452
[Au] Autor:Bosserman RE; Champion PA
[Ad] Endereço:Department of Biological Sciences, Eck Institute for Global Health, Center for Rare and Neglected Diseases, University of Notre Dame, Notre Dame, Indiana, USA.
[Ti] Título:Esx Systems and the Mycobacterial Cell Envelope: What's the Connection?
[So] Source:J Bacteriol;199(17), 2017 09 01.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycobacterial 6-kDa early secreted antigenic target (ESAT-6) system (ESX) exporters transport proteins across the cytoplasmic membrane. Many proteins transported by ESX systems are then translocated across the mycobacterial cell envelope and secreted from the cell. Although the mechanism underlying protein transport across the mycolate outer membrane remains elusive, the ESX systems are closely connected with and localize to the cell envelope. Links between ESX-associated proteins, cell wall synthesis, and the maintenance of cell envelope integrity have been reported. Genes encoding the ESX systems and those required for biosynthesis of the mycobacterial envelope are coregulated. Here, we review the interplay between ESX systems and the mycobacterial cell envelope.
[Mh] Termos MeSH primário: Parede Celular/metabolismo
Mycobacterium/metabolismo
Sistemas de Secreção Tipo VII/metabolismo
[Mh] Termos MeSH secundário: Regulação Bacteriana da Expressão Gênica
Modelos Biológicos
Mycobacterium/genética
Transporte Proteico
Sistemas de Secreção Tipo VII/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Type VII Secretion Systems)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  2 / 12706 MEDLINE  
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[PMID]:29310027
[Au] Autor:Malapati P; Krishna VS; Nallangi R; Srilakshmi RR; Sriram D
[Ad] Endereço:Department of Pharmacy, Birla Institute of Technology & Science-Pilani, Hyderabad Campus, Shameerpet, Jawaharnagar, RangaReddy District, Hyderabad 500 078, India.
[Ti] Título:Identification and development of benzoxazole derivatives as novel bacterial glutamate racemase inhibitors.
[So] Source:Eur J Med Chem;145:23-34, 2018 Feb 10.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:In the present study, we attempted to develop novel class of Mycobacterium tuberculosis (Mtb) inhibitors by exploring the pharmaceutically underexploited enzyme targets which are majorly involved in cell wall biosynthesis of mycobacteria. For this purpose glutamate racemase was selected which racemizes d-glutamate from l-glutamate, a key step in peptidoglycan synthesis. Furthermore, enzyme is neither expressed nor its product, d-glutamate is produced in mammals, and hence inhibiting this enzyme will have no vulnerable effect in host organism. A library of our in-house compounds were screened against glutamate racemase using a biophysical technique; thermal shift assay and further by enzyme inhibition assay to identify Lead 1 molecule. Lead 1 optimization and expansion resulted in twenty four compounds. Among the synthesized compounds twelve compounds shown good enzyme inhibition than Lead 1 (IC 20.07 ±â€¯0.29 µM). Among all the compounds; compound 22 (IC 1.1 ±â€¯0.52 µM) showed potent non-competitive mode of inhibition in enzyme assay. Further showed good susceptibility (in replicating bacteria) of MIC 8.72 µM and bactericidal time dependant kill on dormant culture. It also exhibited significant activity in Mtb nutrient starvation model (2.5) and Mtb biofilm model (2.4) and in vivo M. marinum infected Zebra fish model studies (3.6) reduction at logarithmic scale.
[Mh] Termos MeSH primário: Isomerases de Aminoácido/antagonistas & inibidores
Antibacterianos/farmacologia
Benzoxazóis/farmacologia
Inibidores Enzimáticos/farmacologia
Mycobacterium/efeitos dos fármacos
[Mh] Termos MeSH secundário: Isomerases de Aminoácido/metabolismo
Animais
Antibacterianos/síntese química
Antibacterianos/química
Benzoxazóis/síntese química
Benzoxazóis/química
Biofilmes/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Inibidores Enzimáticos/síntese química
Inibidores Enzimáticos/química
Cinética
Camundongos
Testes de Sensibilidade Microbiana
Simulação de Acoplamento Molecular
Estrutura Molecular
Mycobacterium/enzimologia
Células RAW 264.7
Relação Estrutura-Atividade
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Benzoxazoles); 0 (Enzyme Inhibitors); EC 5.1.1.- (Amino Acid Isomerases); EC 5.1.1.3 (glutamate racemase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


  3 / 12706 MEDLINE  
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[PMID]:29364903
[Au] Autor:Sala A; Cabassi CS; Santospirito D; Polverini E; Flisi S; Cavirani S; Taddei S
[Ad] Endereço:Department of Veterinary Science, University of Parma, Parma, Italy.
[Ti] Título:Novel Naja atra cardiotoxin 1 (CTX-1) derived antimicrobial peptides with broad spectrum activity.
[So] Source:PLoS One;13(1):e0190778, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Naja atra subsp. atra cardiotoxin 1 (CTX-1), produced by Chinese cobra snakes, belonging to Elapidae family, is included in the three-finger toxin family and exerts high cytotoxicity and antimicrobial activity too. Using as template mainly the tip and the subsequent ß-strand of the first "finger" of this toxin, different sequences of 20 amino acids linear peptides have been designed in order to avoid toxic effects but to maintain or even strengthen the partial antimicrobial activity already seen for the complete toxin. As a result, the sequence NCP-0 (Naja Cardiotoxin Peptide-0) was designed as ancestor and subsequently 4 other variant sequences of NCP-0 were developed. These synthesized variant sequences have shown microbicidal activity towards a panel of reference and field strains of Gram-positive and Gram-negative bacteria. The sequence named NCP-3, and its variants NCP-3a and NCP-3b, have shown the best antimicrobial activity, together with low cytotoxicity against eukaryotic cells and low hemolytic activity. Bactericidal activity has been demonstrated by minimum bactericidal concentration (MBC) assay at values below 10 µg/ml for most of the tested bacterial strains. This potent antimicrobial activity was confirmed even for unicellular fungi Candida albicans, Candida glabrata and Malassezia pachydermatis (MBC 50-6.3 µg/ml), and against the fast-growing mycobacteria Mycobacterium smegmatis and Mycobacterium fortuitum. Moreover, NCP-3 has shown virucidal activity on Bovine Herpesvirus 1 (BoHV1) belonging to Herpesviridae family. The bactericidal activity is maintained even in a high salt concentration medium (125 and 250 mM NaCl) and phosphate buffer with 20% Mueller Hinton (MH) medium against E. coli, methicillin resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa reference strains. Considering these in vitro obtained data, the search for active sequences within proteins presenting an intrinsic microbicidal activity could provide a new way for discovering a large number of novel and promising antimicrobial peptides families.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Proteínas Cardiotóxicas de Elapídeos/química
Peptídeos/farmacologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anti-Infecciosos/química
Candida/efeitos dos fármacos
Bovinos
Dicroísmo Circular
Hemólise/efeitos dos fármacos
Herpesvirus Bovino 1/efeitos dos fármacos
Malassezia/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Mycobacterium/efeitos dos fármacos
Naja naja
Peptídeos/química
Conformação Proteica
Ovinos
Staphylococcus aureus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Cobra Cardiotoxin Proteins); 0 (Peptides)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190778


  4 / 12706 MEDLINE  
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[PMID]:28463762
[Au] Autor:Paixão DA; Marzano IM; Jaimes EHL; Pivatto M; Campos DL; Pavan FR; Deflon VM; Maia PIDS; Da Costa Ferreira AM; Uehara IA; Silva MJB; Botelho FV; Pereira-Maia EC; Guilardi S; Guerra W
[Ad] Endereço:Instituto de Química, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
[Ti] Título:Novel copper(II) complexes with hydrazides and heterocyclic bases: Synthesis, structure and biological studies.
[So] Source:J Inorg Biochem;172:138-146, 2017 Jul.
[Is] ISSN:1873-3344
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Five new copper(II) complexes of the type [Cu(NO)(NN)(ClO ) ], in which NO=4-fluorophenoxyacetic acid hydrazide (4-FH) or 4-nitrobenzoic hydrazide (4-NH) and NN=1,10-phenanthroline (phen), 4-4'-dimethoxy-2-2'-bipyridine (dmb) or 2,2-bipyridine (bipy) were synthesized and characterized using various spectroscopic methods. The X-ray structural analysis of one representative compound indicates that the geometry around the copper ion is distorted octahedron, in which the ion is coordinated to hydrazide via the terminal nitrogen and the carbonyl oxygen, and to heterocyclic bases via their two nitrogen atoms. Two perchlorate anions occupy the apical positions, completing the coordination sphere. The cytotoxic activity of compounds was investigated in three tumor cell lines (K562, MDA-MB-231 and MCF-7). Concerning K562 cell line, the complexes with 1,10-phenanthroline exhibit high cytotoxic activity and are more active than carboplatin, free ligands and [Cu(phen) ] . Considering the cytotoxicity results, further investigations for the compounds [Cu(4-FH)(phen)(ClO ) ] I and [Cu(4-NH)(phen)(ClO ) ]∙H O III were performed. Flow cytometric analysis revealed that these complexes induce apoptotic cell death in MDA-MB-231 cell line and bind to DNA with K values of 4.38×10 and 2.62×10 , respectively. These compounds were also evaluated against wild type Mycobacterium tuberculosis (ATCC 27294) and exhibited antimycobacterial activity, displayed MIC values lower than those of the corresponding free ligands.
[Mh] Termos MeSH primário: Complexos de Coordenação/síntese química
Complexos de Coordenação/farmacologia
Cobre/química
Compostos Heterocíclicos/química
Hidrazinas/química
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Antineoplásicos/farmacologia
Antituberculosos/síntese química
Antituberculosos/farmacologia
Apoptose/efeitos dos fármacos
Neoplasias da Mama/tratamento farmacológico
Linhagem Celular Tumoral
Complexos de Coordenação/química
Cristalografia por Raios X
Feminino
Seres Humanos
Concentração Inibidora 50
Células K562
Testes de Sensibilidade Microbiana
Estrutura Molecular
Mycobacterium/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Antitubercular Agents); 0 (Coordination Complexes); 0 (Heterocyclic Compounds); 0 (Hydrazines); 27RFH0GB4R (hydrazine); 789U1901C5 (Copper)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  5 / 12706 MEDLINE  
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[PMID]:28450491
[Ti] Título:Mycobacterium microti infection in a cow in France.
[So] Source:Vet Rec;180(17):429, 2017 04 29.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Doenças dos Bovinos/diagnóstico
Doenças dos Bovinos/virologia
Infecções por Mycobacterium/veterinária
Mycobacterium/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Bovinos
França
Mycobacterium/classificação
Infecções por Mycobacterium/diagnóstico
Infecções por Mycobacterium/virologia
[Pt] Tipo de publicação:CASE REPORTS; LETTER
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1136/vr.j2041


  6 / 12706 MEDLINE  
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[PMID]:29176894
[Au] Autor:Levillain F; Poquet Y; Mallet L; Mazères S; Marceau M; Brosch R; Bange FC; Supply P; Magalon A; Neyrolles O
[Ad] Endereço:Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France.
[Ti] Título:Horizontal acquisition of a hypoxia-responsive molybdenum cofactor biosynthesis pathway contributed to Mycobacterium tuberculosis pathoadaptation.
[So] Source:PLoS Pathog;13(11):e1006752, 2017 Nov.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The unique ability of the tuberculosis (TB) bacillus, Mycobacterium tuberculosis, to persist for long periods of time in lung hypoxic lesions chiefly contributes to the global burden of latent TB. We and others previously reported that the M. tuberculosis ancestor underwent massive episodes of horizontal gene transfer (HGT), mostly from environmental species. Here, we sought to explore whether such ancient HGT played a part in M. tuberculosis evolution towards pathogenicity. We were interested by a HGT-acquired M. tuberculosis-specific gene set, namely moaA1-D1, which is involved in the biosynthesis of the molybdenum cofactor. Horizontal acquisition of this gene set was striking because homologues of these moa genes are present all across the Mycobacterium genus, including in M. tuberculosis. Here, we discovered that, unlike their paralogues, the moaA1-D1 genes are strongly induced under hypoxia. In vitro, a M. tuberculosis moaA1-D1-null mutant has an impaired ability to respire nitrate, to enter dormancy and to survive in oxygen-limiting conditions. Conversely, heterologous expression of moaA1-D1 in the phylogenetically closest non-TB mycobacterium, Mycobacterium kansasii, which lacks these genes, improves its capacity to respire nitrate and grants it with a marked ability to survive oxygen depletion. In vivo, the M. tuberculosis moaA1-D1-null mutant shows impaired survival in hypoxic granulomas in C3HeB/FeJ mice, but not in normoxic lesions in C57BL/6 animals. Collectively, our results identify a novel pathway required for M. tuberculosis resistance to host-imposed stress, namely hypoxia, and provide evidence that ancient HGT bolstered M. tuberculosis evolution from an environmental species towards a pervasive human-adapted pathogen.
[Mh] Termos MeSH primário: Coenzimas/biossíntese
Transferência Genética Horizontal
Metaloproteínas/biossíntese
Mycobacterium tuberculosis/genética
Mycobacterium tuberculosis/metabolismo
Oxigênio/metabolismo
Tuberculose/microbiologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Feminino
Regulação Bacteriana da Expressão Gênica
Seres Humanos
Hipóxia/metabolismo
Hipóxia/microbiologia
Camundongos
Camundongos Endogâmicos C57BL
Mycobacterium/genética
Mycobacterium/metabolismo
Nitratos/metabolismo
Pteridinas
Tuberculose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Coenzymes); 0 (Metalloproteins); 0 (Nitrates); 0 (Pteridines); 73508-07-3 (molybdenum cofactor); S88TT14065 (Oxygen)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006752


  7 / 12706 MEDLINE  
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[PMID]:29236773
[Au] Autor:Jaén-Luchoro D; Aliaga-Lozano F; Gomila RM; Gomila M; Salvà-Serra F; Lalucat J; Bennasar-Figueras A
[Ad] Endereço:Microbiologia, Departament de Biologia, Universitat de les Illes Balears, Palma de Mallorca, Spain.
[Ti] Título:First insights into a type II toxin-antitoxin system from the clinical isolate Mycobacterium sp. MHSD3, similar to epsilon/zeta systems.
[So] Source:PLoS One;12(12):e0189459, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A putative type II toxin-antitoxin (TA) system was found in the clinical isolate Mycobacterium sp. MHSD3, a strain closely related to Mycobacterium chelonae. Further analyses of the protein sequences of the two genes revealed the presence of domains related to a TA system. BLAST analyses indicated the presence of closely related proteins in the genomes of other recently published M. chelonae strains. The functionality of both elements of the TA system was demonstrated when expressed in Escherichia coli cells, and the predicted structure of the toxin is very similar to those of well-known zeta-toxins, leading to the definition of a type II TA system similar to epsilon/zeta TA systems in strains that are closely related to M. chelonae.
[Mh] Termos MeSH primário: Toxinas Bacterianas/metabolismo
Mycobacterium/química
[Mh] Termos MeSH secundário: Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189459


  8 / 12706 MEDLINE  
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[PMID]:29236768
[Au] Autor:Singh B; Saqib M; Gupta A; Kumar P; Bhaskar S
[Ad] Endereço:Product Development Cell, National Institute of Immunology, New Delhi, Delhi, India.
[Ti] Título:Autophagy induction by Mycobacterium indicus pranii promotes Mycobacterium tuberculosis clearance from RAW 264.7 macrophages.
[So] Source:PLoS One;12(12):e0189606, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycobacterium indicus pranii (MIP) is a potent vaccine candidate against tuberculosis (TB) as it has demonstrated significant protection in animal models of tuberculosis as well as in clinical trials. Higher protective efficacy of MIP against TB as compared to BCG provoked the efforts to gain insight into the molecular mechanisms underlying MIP mediated protection against Mycobacterium tuberculosis (M.tb). Autophagy, initially described as a cell survival mechanism during starvation, also plays a key role in host resistance to M.tb. Virulent mycobacteria like M.tb, suppresses host autophagy response to increase its survival in macrophages. Since mycobacterial species have been shown to vary widely in their autophagy-inducing properties, in the present study, we examined the autophagy inducing efficacy of MIP and its role in MIP-mediated protection against M.tb. MIP was found to be potent inducer of autophagy in macrophages. Induced autophagy was responsible for reversal of the phagosome maturation block and phagolysosome fusion inhibition in M.tb infected macrophages, which ultimately lead to significantly enhanced clearance of M.tb from the macrophages. This is an important study which further delineated the underlying mechanisms for significant immunotherapeutic activity observed in TB patients / animal models of tuberculosis, given MIP therapy along with chemotherapy.
[Mh] Termos MeSH primário: Autofagia/fisiologia
Mycobacterium/patogenicidade
[Mh] Termos MeSH secundário: Animais
Camundongos
Células RAW 264.7
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189606


  9 / 12706 MEDLINE  
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[PMID]:29220797
[Au] Autor:Pavic K; Perkovic I; Pospísilová S; Machado M; Fontinha D; Prudêncio M; Jampilek J; Coffey A; Endersen L; Rimac H; Zorc B
[Ad] Endereço:University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovacica 1, 10 000 Zagreb, Croatia.
[Ti] Título:Primaquine hybrids as promising antimycobacterial and antimalarial agents.
[So] Source:Eur J Med Chem;143:769-779, 2018 Jan 01.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Four series of primaquine (PQ) derivatives were screened for antitubercular and antiplasmodial activity: amides 1a-k, ureas 2a-s, semicarbazides 3a-c and bis-ureas 4a-u. Antimycobacterial activity of PQ derivatives against Mycobacterium tuberculosis (MTB), M. avium complex (MAC) and M. avium subsp. paratuberculosis (MAP) were evaluated in vitro and compared with PQ and the standard antitubercular drugs. In general, the PQ derivatives showed higher potency than the parent compound. Most of the compounds of series 1 and 2 showed high activity against MAP, comparable or even higher than the relevant drug ciprofloxacin, and weak or no activity against MTB and MAC. bis-Trifluoromethylated cinnamamide 1k showed low cytotoxicity and high activity against all three Mycobacterium species and their activities were comparable or slightly higher than those of the reference drugs. PQ urea derivatives with hydroxyl, halogen and trifluoromethyl substituents on benzene ring 2f-p exerted very strong antimycobacterial activity towards all tested mycobacteria, stronger than PQ and the relevant standard drug(s). Unfortunately, these compounds had relatively high cytotoxicity, except bromo 2l and trifluoromethyl 2m, 2n derivatives. In general, meta-substituted derivatives were more active than analogues para-derivatives. Phenyl ureas were also more active than cycloalkyl or hydroxyalkyl ureas. Semicarbazide 3a showed similar activity as PQ, while the other two semicarbazides were inactive. Bis-urea derivatives 4 were generally less active than the urea derivatives sharing the same scaffold, differing only in the spacer type. Out of 21 evaluated bis-urea derivatives, only p-Cl/m-CF phenyl derivative 4p, benzhydryl derivatives 4t and 4u and bis-PQ derivative 4s showed high activity, higher than all three reference drugs. After comparison of activity and cytotoxicity, urea 2m and bis-urea 4u could be considered as the most promising agents. Antimalarial potential of PQ derivatives in vitro against the liver stage of P. berghei was evaluated as well. 3-(4-Chlorophenyl)-1-[({4-[(6-methoxyquinolin-8-yl)amino]pentyl}carbamoyl)amino]urea (4l) was the most active compound (IC = 42 nM; cytotoxicity/activity ratio >2000). Our results bring new insights into development of novel anti-TB and antimalarial compounds.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antimaláricos/farmacologia
Mycobacterium/efeitos dos fármacos
Plasmodium berghei/efeitos dos fármacos
Primaquina/farmacologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/síntese química
Antibacterianos/química
Antimaláricos/síntese química
Antimaláricos/química
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Testes de Sensibilidade Microbiana
Estrutura Molecular
Mycobacterium/crescimento & desenvolvimento
Plasmodium berghei/crescimento & desenvolvimento
Primaquina/síntese química
Primaquina/química
Ratos
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antimalarials); MVR3634GX1 (Primaquine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE


  10 / 12706 MEDLINE  
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[PMID]:28968436
[Au] Autor:Li Y; Chen W; Wang Y; Luo K; Li Y; Bai L; Luo F
[Ad] Endereço:College of Plant Protection, Hunan Agricultural University, Changsha, China.
[Ti] Título:Identifying and sequencing a Mycobacterium sp. strain F4 as a potential bioremediation agent for quinclorac.
[So] Source:PLoS One;12(10):e0185721, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quinclorac is a widely used herbicide in rice filed. Unfortunately, quinclorac residues are phytotoxic to many crops/vegetables. The degradation of quinclorac in nature is very slow. On the other hand, degradation of quinclorac using bacteria can be an effective and efficient method to reduce its contamination. In this study, we isolated a quinclorac bioremediation bacterium strain F4 from quinclorac contaminated soils. Based on morphological characteristics and 16S rRNA gene sequence analysis, we identified strain F4 as Mycobacterium sp. We investigated the effects of temperature, pH, inoculation size and initial quinclorac concentration on growth and degrading efficiency of F4 and determined the optimal quinclorac degrading condition of F4. Under optimal degrading conditions, F4 degraded 97.38% of quinclorac from an initial concentration of 50 mg/L in seven days. Our indoor pot experiment demonstrated that the degradation products were non-phytotoxic to tobacco. After analyzing the quinclorac degradation products of F4, we proposed that F4 could employ two pathways to degrade quinclorac: one is through methylation, the other is through dechlorination. Furthermore, we reconstructed the whole genome of F4 through single molecular sequencing and de novo assembly. We identified 77 methyltransferases and eight dehalogenases in the F4 genome to support our hypothesized degradation path.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Herbicidas/metabolismo
Mycobacterium/metabolismo
Quinolinas/metabolismo
[Mh] Termos MeSH secundário: Genes Bacterianos
Concentração de Íons de Hidrogênio
Mycobacterium/genética
Mycobacterium/isolamento & purificação
RNA Ribossômico 16S/genética
Poluentes do Solo/metabolismo
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 0 (Quinolines); 0 (RNA, Ribosomal, 16S); 0 (Soil Pollutants); 3J06V625EE (quinclorac)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185721



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