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  1 / 2513 MEDLINE  
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[PMID]:29366791
[Au] Autor:Ganareal TACS; Balbin MM; Monserate JJ; Salazar JR; Mingala CN
[Ad] Endereço:Department of Chemistry, College of Arts and Sciences, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines.
[Ti] Título:Gold nanoparticle-based probes for the colorimetric detection of Mycobacterium avium subspecies paratuberculosis DNA.
[So] Source:Biochem Biophys Res Commun;496(3):988-997, 2018 02 12.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gold nanoparticle (AuNP) is considered to be the most stable metal nanoparticle having the ability to be functionalized with biomolecules. Recently, AuNP-based DNA detection methods captured the interest of researchers worldwide. Paratuberculosis or Johne's disease, a chronic gastroenteritis in ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP), was found to have negative effect in the livestock industry. In this study, AuNP-based probes were evaluated for the specific and sensitive detection of MAP DNA. AuNP-based probe was produced by functionalization of AuNPs with thiol-modified oligonucleotide and was confirmed by Fourier-Transform Infrared (FTIR) spectroscopy. UV-Vis spectroscopy and Scanning Electron Microscopy (SEM) were used to characterize AuNPs. DNA detection was done by hybridization of 10 µL of DNA with 5 µL of probe at 63 °C for 10 min and addition of 3 µL salt solution. The method was specific to MAP with detection limit of 103 ng. UV-Vis and SEM showed dispersion and aggregation of the AuNPs for the positive and negative results, respectively, with no observed particle growth. This study therefore reports an AuNP-based probes which can be used for the specific and sensitive detection of MAP DNA.
[Mh] Termos MeSH primário: Colorimetria/métodos
DNA/genética
DNA/isolamento & purificação
Ouro/química
Nanopartículas Metálicas/química
Mycobacterium avium/genética
Mycobacterium avium/isolamento & purificação
[Mh] Termos MeSH secundário: Técnicas Biossensoriais/métodos
Hibridização In Situ/métodos
Técnicas de Sonda Molecular
Sondas Moleculares/química
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Molecular Probes); 7440-57-5 (Gold); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


  2 / 2513 MEDLINE  
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[PMID]:28837698
[Au] Autor:Dong H; Lv Y; Sreevatsan S; Zhao D; Zhou X
[Ad] Endereço:State Key Laboratory of Agrobiotechnology, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and College of Veterinary Medicine, China Agricultural University, Beijing, China.
[Ti] Título:Differences in pathogenicity of three animal isolates of Mycobacterium species in a mouse model.
[So] Source:PLoS One;12(8):e0183666, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Animal mycobacterioses are among the most important zoonoses worldwide. These are generally caused by either Mycobacterium tuberculosis (MTB), M. bovis (MBO) or M. avium (MAV). To test the hypothesis that different species of pathogenic mycobacteria isolated from varied anatomic locations or animal species differ in virulence and pathogenicity, we performed experiments with three mycobacteria strains (NTSE-3(MTB), NTSE-4(MBO) and NTSE-5 (MAV)) obtained from animal species. Spoligotyping analysis was used to confirm both MTB and MBO strains while the MAV strain was confirmed by 16s rDNA sequencing. BALB/c mice were intranasally infected with the three strains at low and high CFU doses to evaluate variations in pathogenicity. Clinical and pathological parameters were assessed. Infected mice were euthanized at 80 days post-inoculation (dpi). Measures of lung and body weights indicated that the MBO infected group had higher mortality, more weight loss, higher bacterial burden and more severe lesions in lungs than the other two groups. Cytokine profiles showed higher levels of TNF-α for MBO versus MTB, while MAV had the highest amounts of IFN-ß in vitro and in vivo. In vitro levels of other cytokines such as IL-1ß, IL-10, IL-12, IL-17, and IFN-ß showed that Th1 cells had the strongest response in MBO infected mice and that Th2 cells were inhibited. We found that the level of virulence among the three isolates decreased in the following order MBO>MTB>MAV.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Infecções por Mycobacterium/microbiologia
Mycobacterium avium/patogenicidade
Mycobacterium bovis/patogenicidade
Mycobacterium tuberculosis/patogenicidade
[Mh] Termos MeSH secundário: Animais
Peso Corporal
Citocinas/metabolismo
Feminino
Pulmão/microbiologia
Pulmão/patologia
Camundongos
Camundongos Endogâmicos BALB C
Infecções por Mycobacterium/metabolismo
Tamanho do Órgão
Especificidade da Espécie
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183666


  3 / 2513 MEDLINE  
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[PMID]:28806745
[Au] Autor:Gidon A; Åsberg SE; Louet C; Ryan L; Haug M; Flo TH
[Ad] Endereço:Centre of Molecular Inflammation Research and Department of Cancer Research and Molecular Medicine, Faculty of Medicine, NTNU, Norwegian University of Science and Technology, Trondheim, Norway.
[Ti] Título:Persistent mycobacteria evade an antibacterial program mediated by phagolysosomal TLR7/8/MyD88 in human primary macrophages.
[So] Source:PLoS Pathog;13(8):e1006551, 2017 Aug.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pathogenic mycobacteria reside in macrophages where they avoid lysosomal targeting and degradation through poorly understood mechanisms proposed to involve arrest of phagosomal maturation at an early endosomal stage. A clear understanding of how this relates to host defenses elicited from various intracellular compartments is also missing and can only be studied using techniques allowing single cell and subcellular analyses. Using confocal imaging of human primary macrophages infected with Mycobacterium avium (Mav) we show evidence that Mav phagosomes are not arrested at an early endosomal stage, but mature to a (LAMP1+/LAMP2+/CD63+) late endosomal/phagolysosomal stage where inflammatory signaling and Mav growth restriction is initiated through a mechanism involving Toll-like receptors (TLR) 7 and 8, the adaptor MyD88 and transcription factors NF-κB and IRF-1. Furthermore, a fraction of the mycobacteria re-establish in a less hostile compartment (LAMP1-/LAMP2-/CD63-) where they not only evade destruction, but also recognition by TLRs, growth restriction and inflammatory host responses that could be detrimental for intracellular survival and establishment of chronic infections.
[Mh] Termos MeSH primário: Macrófagos/microbiologia
Infecções por Mycobacterium/imunologia
Fator 88 de Diferenciação Mieloide/imunologia
Receptor 7 Toll-Like/imunologia
[Mh] Termos MeSH secundário: Seres Humanos
Processamento de Imagem Assistida por Computador
Imuno-Histoquímica
Lisossomos/imunologia
Macrófagos/imunologia
Microscopia Confocal
Mycobacterium avium
Fagossomos/imunologia
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MYD88 protein, human); 0 (Myeloid Differentiation Factor 88); 0 (TLR7 protein, human); 0 (Toll-Like Receptor 7)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006551


  4 / 2513 MEDLINE  
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[PMID]:28687660
[Au] Autor:Resende M; Cardoso MS; Ribeiro AR; Flórido M; Borges M; Castro AG; Alves NL; Cooper AM; Appelberg R
[Ad] Endereço:IBMC - Instituto de Biologia Molecular e Celular and i3S - Instituto de Investigação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal; mrsilva@ibmc.up.pt.
[Ti] Título:Innate IFN-γ-Producing Cells Developing in the Absence of IL-2 Receptor Common γ-Chain.
[So] Source:J Immunol;199(4):1429-1439, 2017 Aug 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:IFN-γ is known to be predominantly produced by lymphoid cells such as certain subsets of T cells, NK cells, and other group 1 innate lymphoid cells. In this study, we used IFN-γ reporter mouse models to search for additional cells capable of secreting this cytokine. We identified a novel and rare population of nonconventional IFN-γ-producing cells of hematopoietic origin that were characterized by the expression of Thy1.2 and the lack of lymphoid, myeloid, and NK lineage markers. The expression of IFN-γ by this population was higher in the liver and lower in the spleen. Furthermore, these cells were present in mice lacking both the and the common γ-chain (γc) genes (Rag2 γc ), indicating their innate nature and their γc cytokine independence. Rag2 γc mice are as resistant to as Rag2 mice, whereas Rag2 mice lacking IFN-γ are more susceptible than either or Rag2 γc These lineage-negative CD45 /Thy1.2 cells are found within the mycobacterially induced granulomatous structure in the livers of infected Rag2 γc animals and are adjacent to macrophages that expressed inducible NO synthase, suggesting a potential protective role for these IFN-γ-producing cells. Accordingly, Thy1.2-specific mAb administration to infected Rag2 γc animals increased growth in the liver. Overall, our results demonstrate that a population of Thy1.2 non-NK innate-like cells present in the liver expresses IFN-γ and can confer protection against infection in immunocompromised mice.
[Mh] Termos MeSH primário: Células-Tronco Hematopoéticas/imunologia
Imunidade Inata
Interferon gama/biossíntese
Interferon gama/genética
Subunidade gama Comum de Receptores de Interleucina/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/administração & dosagem
Proteínas de Ligação a DNA/deficiência
Proteínas de Ligação a DNA/genética
Proteínas de Ligação a DNA/imunologia
Granuloma/imunologia
Granuloma/microbiologia
Hospedeiro Imunocomprometido/imunologia
Interferon gama/imunologia
Subunidade gama Comum de Receptores de Interleucina/deficiência
Subunidade gama Comum de Receptores de Interleucina/genética
Células Matadoras Naturais/imunologia
Antígenos Comuns de Leucócito/genética
Antígenos Comuns de Leucócito/imunologia
Fígado/citologia
Fígado/imunologia
Fígado/microbiologia
Macrófagos/imunologia
Camundongos
Camundongos Endogâmicos C57BL
Mycobacterium avium/crescimento & desenvolvimento
Mycobacterium avium/imunologia
Óxido Nítrico Sintase Tipo II/biossíntese
Óxido Nítrico Sintase Tipo II/genética
Óxido Nítrico Sintase Tipo II/imunologia
Baço/citologia
Baço/imunologia
Antígenos Thy-1/genética
Antígenos Thy-1/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (DNA-Binding Proteins); 0 (Interleukin Receptor Common gamma Subunit); 0 (Rag2 protein, mouse); 0 (Thy-1 Antigens); 82115-62-6 (Interferon-gamma); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nos2 protein, mouse); EC 3.1.3.48 (Leukocyte Common Antigens); EC 3.1.3.48 (Ptprc protein, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170709
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601701


  5 / 2513 MEDLINE  
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[PMID]:28671535
[Au] Autor:Jeffrey B; Rose SJ; Gilbert K; Lewis M; Bermudez LE
[Ad] Endereço:1​Department of Biomedical Sciences, College of Veterinary Medicine, Corvallis, Oregon, USA.
[Ti] Título:Comparative analysis of the genomes of clinical isolates of Mycobacterium avium subsp. hominissuis regarding virulence-related genes.
[So] Source:J Med Microbiol;66(7):1063-1075, 2017 Jul.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Mycobacterium avium subsp. hominissuis is a member of the M. avium complex, a heterogeneous group of bacteria that cause lung infection in immunocompetent patients or disseminated infection in patients with immunosuppression. The bacteria belonging to this complex have variable virulence, depending on the strain considered, and therefore a representative of the most common clinical phenotype was analysed. METHODOLOGY: The genomic sequences of four M. avium subsp. hominissuis isolates obtained from clinical specimens were completed. Mav101, Mav100 and MavA5 were isolated from the blood of patients with AIDS. MavA5 was disseminated from the lung, while Mav3388 was isolated from the lungs of a patient with chronic lung disease. The sequences were annotated using the published Mav104 genome as a blueprint. Functional and virulence analyses of the sequences were carried out. Mice studies comparing the virulence of the strains were performed. RESULTS: Findings showed that while Mav101 was very similar to Mav104, there were numerous differences between Mav104 and the remaining strains at nucleotide and predicted protein levels. The presence of genes associated with biofilm formation and several known virulence-related genes were sometimes differentially present among the isolates, suggesting overlapping functions by different genetic determinants. CONCLUSIONS: The sequences provided important information about M. avium heterogenicity and evolution as a pathogen. The limitation is the lack of understanding on possible overlapping functions of genes/proteins.
[Mh] Termos MeSH primário: Variação Genética
Genoma Bacteriano
Mycobacterium avium/genética
Mycobacterium avium/isolamento & purificação
Tuberculose/microbiologia
Fatores de Virulência/genética
[Mh] Termos MeSH secundário: Síndrome de Imunodeficiência Adquirida/complicações
Animais
Modelos Animais de Doenças
Seres Humanos
Camundongos Endogâmicos C57BL
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Virulence Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000507


  6 / 2513 MEDLINE  
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[PMID]:28267758
[Au] Autor:Pfeiffer W; Braun J; Burchell J; Witte CL; Rideout BA
[Ad] Endereço:San Diego Supercomputer Center, University of California, San Diego, La Jolla, California, United States of America.
[Ti] Título:Whole-genome analysis of mycobacteria from birds at the San Diego Zoo.
[So] Source:PLoS One;12(3):e0173464, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:METHODS: Mycobacteria isolated from more than 100 birds diagnosed with avian mycobacteriosis at the San Diego Zoo and its Safari Park were cultured postmortem and had their whole genomes sequenced. Computational workflows were developed and applied to identify the mycobacterial species in each DNA sample, to find single-nucleotide polymorphisms (SNPs) between samples of the same species, to further differentiate SNPs between as many as three different genotypes within a single sample, and to identify which samples are closely clustered genomically. RESULTS: Nine species of mycobacteria were found in 123 samples from 105 birds. The most common species were Mycobacterium avium and Mycobacterium genavense, which were in 49 and 48 birds, respectively. Most birds contained only a single mycobacterial species, but two birds contained a mixture of two species. The M. avium samples represent diverse strains of M. avium avium and M. avium hominissuis, with many pairs of samples differing by hundreds or thousands of SNPs across their common genome. By contrast, the M. genavense samples are much closer genomically; samples from 46 of 48 birds differ from each other by less than 110 SNPs. Some birds contained two, three, or even four genotypes of the same bacterial species. Such infections were found in 4 of 49 birds (8%) with M. avium and in 11 of 48 birds (23%) with M. genavense. Most were mixed infections, in which the bird was infected by multiple mycobacterial strains, but three infections with two genotypes differing by ≤ 10 SNPs were likely the result of within-host evolution. The samples from 31 birds with M. avium can be grouped into nine clusters within which any sample is ≤ 12 SNPs from at least one other sample in the cluster. Similarly, the samples from 40 birds with M. genavense can be grouped into ten such clusters. Information about these genomic clusters is being used in an ongoing, companion study of mycobacterial transmission to help inform management of bird collections.
[Mh] Termos MeSH primário: Doenças das Aves/microbiologia
Genoma Bacteriano
Genômica
Infecções por Mycobacterium/veterinária
Mycobacterium/classificação
Mycobacterium/genética
[Mh] Termos MeSH secundário: Animais
California
Biologia Computacional/métodos
Bases de Dados de Ácidos Nucleicos
Genômica/métodos
Genótipo
Sequenciamento de Nucleotídeos em Larga Escala
Mycobacterium avium/genética
Filogenia
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173464


  7 / 2513 MEDLINE  
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[PMID]:28182785
[Au] Autor:Bruffaerts N; Vluggen C; Roupie V; Duytschaever L; Van den Poel C; Denoël J; Wattiez R; Letesson JJ; Fretin D; Rigouts L; Chapeira O; Mathys V; Saegerman C; Huygen K
[Ad] Endereço:Service Immunology, Operational Direction Communicable and infectious Diseases, Scientific Institute of Public Health, Brussels, Belgium.
[Ti] Título:Virulence and immunogenicity of genetically defined human and porcine isolates of M. avium subsp. hominissuis in an experimental mouse infection.
[So] Source:PLoS One;12(2):e0171895, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycobacterium avium subsp. hominissuis (Mah) represents a health concern for humans and to a lesser extent for pigs, but its zoonotic potential remains elusive. Using multispacer sequence typing (MST) we previously identified 49 different genotypes of Mah among Belgian clinical and porcine isolates, with 5 MSTs shared by both hosts. Using experimental intranasal infection of BALB/c mice, we compared the virulence and immunogenicity of porcine and clinical human isolates with shared genotype or with a genotype only found in humans or pigs. Bacterial replication was monitored for 20 weeks in lungs, spleen and liver and mycobacteria specific spleen cell IFN-γ, IL-10 and IL-17 production as well as serum antibody responses were analyzed. Isolates varied in virulence, with human and porcine isolates sharing MST22 genotype showing a thousand fold higher bacterial replication in lungs and more dissemination to spleen and liver than the human and porcine MST91 isolates. Virulent MST22 type was also associated with progressive suppression of IFN-γ and IL-17 responses, and increased IL-10 production. Whole genome sequencing of the two virulent isolates with MST22 genotype and two avirulent isolates of genotype MST91 and comparison with two well-studied M. avium subsp. hominissuis reference strains i.e. Mah 104 and Mah TH135, identified in the two MST22 isolates nine specific virulence factors of the mammalian cell entry family, that were identical with Mah 104 strain. Despite the obvious limitations of the mouse model, a striking link of virulence and identity at the genome level of porcine and human isolates with the same multisequence type, for which no correlation of place of residence (humans) or farm of origin (pigs) was observed, seems to point to the existence in the environment of certain genotypes of Mah which may be more infectious both for humans and pigs than other genotypes.
[Mh] Termos MeSH primário: Genótipo
Mycobacterium avium/genética
Tuberculose/microbiologia
[Mh] Termos MeSH secundário: Adulto
Animais
Pré-Escolar
Feminino
Genoma Bacteriano
Seres Humanos
Interferon gama/genética
Interferon gama/metabolismo
Interleucinas/genética
Interleucinas/metabolismo
Fígado/microbiologia
Pulmão/microbiologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Mycobacterium avium/imunologia
Mycobacterium avium/isolamento & purificação
Mycobacterium avium/patogenicidade
Baço/microbiologia
Suínos
Tuberculose/imunologia
Tuberculose/veterinária
Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukins); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171895


  8 / 2513 MEDLINE  
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[PMID]:28156101
[Au] Autor:Swarbrick CM; Bythrow GV; Aragao D; Germain GA; Quadri LE; Forwood JK
[Ad] Endereço:School of Biomedical Sciences, Charles Sturt University , Wagga Wagga, New South Wales 2678, Australia.
[Ti] Título:Mycobacteria Encode Active and Inactive Classes of TesB Fatty-Acyl CoA Thioesterases Revealed through Structural and Functional Analysis.
[So] Source:Biochemistry;56(10):1460-1472, 2017 Mar 14.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycobacteria contain a large number of highly divergent species and exhibit unusual lipid metabolism profiles, believed to play important roles in immune invasion. Thioesterases modulate lipid metabolism through the hydrolysis of activated fatty-acyl CoAs; multiple copies are present in mycobacteria, yet many remain uncharacterized. Here, we undertake a comprehensive structural and functional analysis of a TesB thioesterase from Mycobacterium avium (MaTesB). Structural superposition with other TesB thioesterases reveals that the Asp active site residue, highly conserved across a wide range of TesB thioesterases, is mutated to Ala. Consistent with these structural data, the wild-type enzyme failed to hydrolyze an extensive range of acyl-CoA substrates. Mutation of this residue to an active Asp residue restored activity against a range of medium-chain length fatty-acyl CoA substrates. Interestingly, this Ala mutation is highly conserved across a wide range of Mycobacterium species but not found in any other bacteria or organism. Our structural homology analysis revealed that at least one other TesB acyl-CoA thioesterase also contains an Ala residue at the active site, while two other Mycobacterium TesB thioesterases harbor an Asp residue at the active site. The inactive TesBs display a common quaternary structure that is distinct from that of the active TesB thioesterases. Investigation of the effect of expression of either the catalytically active or inactive MaTesB in Mycobacterium smegmatis exposed, to the best of our knowledge, the first genotype-phenotype association implicating a mycobacterial tesB gene. This is the first report that mycobacteria encode active and inactive forms of thioesterases, the latter of which appear to be unique to mycobacteria.
[Mh] Termos MeSH primário: Acil Coenzima A/química
Proteínas de Bactérias/química
Mycobacterium avium/enzimologia
Mycobacterium smegmatis/enzimologia
Palmitoil-CoA Hidrolase/química
[Mh] Termos MeSH secundário: Acil Coenzima A/metabolismo
Alanina/química
Alanina/metabolismo
Sequência de Aminoácidos
Substituição de Aminoácidos
Ácido Aspártico/química
Ácido Aspártico/metabolismo
Proteínas de Bactérias/classificação
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Domínio Catalítico
Escherichia coli/enzimologia
Escherichia coli/genética
Expressão Gênica
Estudos de Associação Genética
Hidrólise
Isoenzimas/química
Isoenzimas/classificação
Isoenzimas/genética
Isoenzimas/metabolismo
Cinética
Mutação
Mycobacterium avium/genética
Mycobacterium smegmatis/genética
Palmitoil-CoA Hidrolase/classificação
Palmitoil-CoA Hidrolase/genética
Palmitoil-CoA Hidrolase/metabolismo
Domínios Proteicos
Estrutura Quaternária de Proteína
Estrutura Secundária de Proteína
Proteínas Recombinantes/química
Proteínas Recombinantes/classificação
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acyl Coenzyme A); 0 (Bacterial Proteins); 0 (Isoenzymes); 0 (Recombinant Proteins); 30KYC7MIAI (Aspartic Acid); EC 3.1.2.2 (Palmitoyl-CoA Hydrolase); OF5P57N2ZX (Alanine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170616
[Lr] Data última revisão:
170616
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.6b01049


  9 / 2513 MEDLINE  
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[PMID]:27915187
[Au] Autor:Hamilton KA; Weir MH; Haas CN
[Ad] Endereço:Department of Civil, Architectural, and Environmental Engineering, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, USA. Electronic address: kh495@drexel.edu.
[Ti] Título:Dose response models and a quantitative microbial risk assessment framework for the Mycobacterium avium complex that account for recent developments in molecular biology, taxonomy, and epidemiology.
[So] Source:Water Res;109:310-326, 2017 Feb 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mycobacterium avium complex (MAC) is a group of environmentally-transmitted pathogens of great public health importance. This group is known to be harbored, amplified, and selected for more human-virulent characteristics by amoeba species in aquatic biofilms. However, a quantitative microbial risk assessment (QMRA) has not been performed due to the lack of dose response models resulting from significant heterogeneity within even a single species or subspecies of MAC, as well as the range of human susceptibilities to mycobacterial disease. The primary human-relevant species and subspecies responsible for the majority of the human disease burden and present in drinking water, biofilms, and soil are M. avium subsp. hominissuis, M. intracellulare, and M. chimaera. A critical review of the published literature identified important health endpoints, exposure routes, and susceptible populations for MAC risk assessment. In addition, data sets for quantitative dose-response functions were extracted from published in vivo animal dosing experiments. As a result, seven new exponential dose response models for human-relevant species of MAC with endpoints of lung lesions, death, disseminated infection, liver infection, and lymph node lesions are proposed. Although current physical and biochemical tests used in clinical settings do not differentiate between M. avium and M. intracellulare, differentiating between environmental species and subspecies of the MAC can aid in the assessment of health risks and control of MAC sources. A framework is proposed for incorporating the proposed dose response models into susceptible population- and exposure route-specific QMRA models.
[Mh] Termos MeSH primário: Complexo Mycobacterium avium
Infecção por Mycobacterium avium-intracellulare
[Mh] Termos MeSH secundário: Animais
Biofilmes
Água Potável
Seres Humanos
Mycobacterium avium
Medição de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Drinking Water)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161205
[St] Status:MEDLINE


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[PMID]:27868301
[Au] Autor:Ricchi M; Mazzarelli A; Piscini A; Di Caro A; Cannas A; Leo S; Russo S; Arrigoni N
[Ad] Endereço:Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, National Reference Centre for Paratuberculosis, Gariga di Podenzano, Italy.
[Ti] Título:Exploring MALDI-TOF MS approach for a rapid identification of Mycobacterium avium ssp. paratuberculosis field isolates.
[So] Source:J Appl Microbiol;122(3):568-577, 2017 Mar.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: The aim of the study was to explore the suitability of matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) for a rapid and correct identification of Mycobacterium avium ssp. paratuberculosis (MAP) field isolates. METHODS AND RESULTS: MALDI-TOF MS approach is becoming one of the most popular tests for the identification of intact bacterial cells which has been shown to be fast and reliable. For this purpose, 36 MAP field isolates were analysed through MALDI-TOF MS and the spectra compared with two different databases: one provided by the vendor of the system employed (Biotyper ver. 3·0; Bruker Daltonics) and a homemade database containing spectra from both tuberculous and nontuberculous Mycobacteria. Moreover, principal component analysis procedure was employed to confirm the ability of MALDI-TOF MS to discriminate between very closely related subspecies. Our results suggest MAP can be differentiated from other Mycobacterium species, both when the species are very close (M. intracellulare) and when belonging to different subspecies (M. avium ssp. avium and M. avium ssp. silvaticum). CONCLUSIONS: The procedure applied is fast, easy to perform, and achieves an earlier accurate species identification of MAP and nontuberculous Mycobacteria in comparison to other procedures. SIGNIFICANCE AND IMPACT OF THE STUDY: The gold standard test for the diagnosis of paratuberculosis is still isolation of MAP by cultural methods, but additional assays, such as qPCR and subculturing for determination of mycobactin dependency are required to confirm its identification. We have provided here evidence pertaining to the usefulness of MALDI-TOF MS approach for a rapid identification of this mycobacterium among other members of M. avium complex.
[Mh] Termos MeSH primário: Mycobacterium avium subsp. paratuberculosis/metabolismo
Mycobacterium avium/classificação
Paratuberculose/diagnóstico
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/química
Mycobacterium avium/isolamento & purificação
Mycobacterium avium/metabolismo
Mycobacterium avium subsp. paratuberculosis/isolamento & purificação
Paratuberculose/microbiologia
Análise de Componente Principal
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170615
[Lr] Data última revisão:
170615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161122
[St] Status:MEDLINE
[do] DOI:10.1111/jam.13357



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