Base de dados : MEDLINE
Pesquisa : B03.510.024.049.525.500.502 [Categoria DeCS]
Referências encontradas : 5143 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 515 ir para página                         

  1 / 5143 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Suffys, Philip N
Texto completo
[PMID]:29367657
[Au] Autor:Benjak A; Avanzi C; Singh P; Loiseau C; Girma S; Busso P; Fontes ANB; Miyamoto Y; Namisato M; Bobosha K; Salgado CG; da Silva MB; Bouth RC; Frade MAC; Filho FB; Barreto JG; Nery JAC; Bührer-Sékula S; Lupien A; Al-Samie AR; Al-Qubati Y; Alkubati AS; Bretzel G; Vera-Cabrera L; Sakho F; Johnson CR; Kodio M; Fomba A; Sow SO; Gado M; Konaté O; Stefani MMA; Penna GO; Suffys PN; Sarno EN; Moraes MO; Rosa PS; Baptista IMFD; Spencer JS; Aseffa A; Matsuoka M; Kai M; Cole ST
[Ad] Endereço:Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, 1015, Switzerland.
[Ti] Título:Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae.
[So] Source:Nat Commun;9(1):352, 2018 01 24.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Leprosy is a chronic human disease caused by the yet-uncultured pathogen Mycobacterium leprae. Although readily curable with multidrug therapy (MDT), over 200,000 new cases are still reported annually. Here, we obtain M. leprae genome sequences from DNA extracted directly from patients' skin biopsies using a customized protocol. Comparative and phylogenetic analysis of 154 genomes from 25 countries provides insight into evolution and antimicrobial resistance, uncovering lineages and phylogeographic trends, with the most ancestral strains linked to the Far East. In addition to known MDT-resistance mutations, we detect other mutations associated with antibiotic resistance, and retrace a potential stepwise emergence of extensive drug resistance in the pre-MDT era. Some of the previously undescribed mutations occur in genes that are apparently subject to positive selection, and two of these (ribD, fadD9) are restricted to drug-resistant strains. Finally, nonsense mutations in the nth excision repair gene are associated with greater sequence diversity and drug resistance.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Farmacorresistência Bacteriana/genética
Mycobacterium leprae/efeitos dos fármacos
Filogenia
[Mh] Termos MeSH secundário: Códon sem Sentido
DNA Bacteriano/química
Genoma Bacteriano
Seres Humanos
Testes de Sensibilidade Microbiana
Mycobacterium leprae/genética
Mycobacterium leprae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Codon, Nonsense); 0 (DNA, Bacterial)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02576-z


  2 / 5143 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Suffys, Philip N
Texto completo
[PMID]:29244821
[Au] Autor:Fontes ANB; Lima LNGC; Mota RMS; Almeida RLF; Pontes MA; Gonçalves HS; Frota CC; Vissa VD; Brennan PJ; Guimaraes RJPS; Kendall C; Kerr LRFS; Suffys PN
[Ad] Endereço:Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.
[Ti] Título:Genotyping of Mycobacterium leprae for better understanding of leprosy transmission in Fortaleza, Northeastern Brazil.
[So] Source:PLoS Negl Trop Dis;11(12):e0006117, 2017 12.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Leprosy is endemic in large part of Brazil with 28,761 new patients in 2015, the second largest number worldwide and reaches 9/10.000 in highly endemic regions and 2.7/10.000 in the city of Fortaleza, Ceará, Northeast Brazil. For better understanding of risk factors for leprosy transmission, we conducted an epidemiologic study supplemented by 17 locus VNTR and SNP 1-4 typing of Mycobacterium leprae in skin biopsy samples from new multibacillary (MB) patients diagnosed at a reference center in 2009 and 2010. Among the 1,519 new patients detected during the study period, 998 (65.7%) were MB and we performed DNA extraction and genotyping on 160 skin biopsy samples, resulting in 159 (16%) good multilocus VNTR types. Thirty-eight of these patients also provided VNTR types from M. leprae in nasal swabs. The SNP-Type was obtained for 157 patients and 87% were of type 4. Upon consideration all VNTR markers, 156 different genotypes and three pairs with identical genotypes were observed; no epidemiologic relation could be observed between individuals in these pairs. Considerable variability in differentiating index (DI) was observed between the different markers and the four with highest DI [(AT)15, (TA)18, (AT)17 and (GAA)21] frequently demonstrated differences in copy number when comparing genotypes from both type of samples. Excluding these markers from analysis resulted in 83 genotypes, 20 of which included 96 of the patients (60.3%). These clusters were composed of two (n = 8), three (n = 6), four (n = 1), five (n = 2), six (n = 1), 19 (n = 1) and 23 (n = 23) individuals and suggests that recent transmission is contributing to the maintenance of leprosy in Fortaleza. When comparing epidemiological and clinical variables among patients within clustered or with unique M. leprae genotypes, a positive bacterial index in skin biopsies and knowledge of working with someone with the disease were significantly associated with clustering. A tendency to belong to a cluster was observed with later notification of disease (mean value of 3.4 months) and having disability grade 2. A tendency for lack of clustering was observed for patients who reported to have lived with another leprosy case but this might be due to lack of inclusion of household contacts in the study. Although clusters were spread over the city, kernel analysis revealed that some of the patients belonging to the two major clusters were spatially related to some neighborhoods that report poverty and high disease incidence in children. Finally, inclusion of genotypes from nasal swabs might be warranted. A major limitation of the study is that sample size of 160 patients from a two year period represents only 15% of the new patients and this could have weakened statistical outcomes. This is the first molecular epidemiology study of leprosy in Brazil and although the high clustering level suggests that recent transmission is the major cause of disease in Fortaleza; the existence of two large clusters needs further investigation.
[Mh] Termos MeSH primário: Hanseníase/transmissão
Mycobacterium leprae/genética
[Mh] Termos MeSH secundário: Brasil/epidemiologia
Análise por Conglomerados
Genótipo
Geografia
Seres Humanos
Hanseníase/microbiologia
Repetições Minissatélites/genética
Epidemiologia Molecular
Fatores de Risco
Análise Espacial
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006117


  3 / 5143 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29228004
[Au] Autor:Richardus RA; van der Zwet K; van Hooij A; Wilson L; Oskam L; Faber R; van den Eeden SJF; Pahan D; Alam K; Richardus JH; Geluk A
[Ad] Endereço:Department of Infectious Diseases Leiden University Medical Center, Leiden, The Netherlands.
[Ti] Título:Longitudinal assessment of anti-PGL-I serology in contacts of leprosy patients in Bangladesh.
[So] Source:PLoS Negl Trop Dis;11(12):e0006083, 2017 12.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Despite elimination efforts, the number of Mycobacterium leprae (M. leprae) infected individuals who develop leprosy, is still substantial. Solid evidence exists that individuals living in close proximity to patients are at increased risk to develop leprosy. Early diagnosis of leprosy in endemic areas requires field-friendly tests that identify individuals at risk of developing the disease before clinical manifestation. Such assays will simultaneously contribute to reduction of current diagnostic delay as well as transmission. Antibody (Ab) levels directed against the M.leprae-specific phenolic glycolipid I (PGL-I) represents a surrogate marker for bacterial load. However, it is insufficiently defined whether anti-PGL-I antibodies can be utilized as prognostic biomarkers for disease in contacts. Particularly, in Bangladesh, where paucibacillary (PB) patients form the majority of leprosy cases, anti-PGL-I serology is an inadequate method for leprosy screening in contacts as a directive for prophylactic treatment. METHODS: Between 2002 and 2009, fingerstick blood from leprosy patients' contacts without clinical signs of disease from a field-trial in Bangladesh was collected on filter paper at three time points covering six years of follow-up per person. Analysis of anti-PGL-I Ab levels for 25 contacts who developed leprosy during follow-up and 199 contacts who were not diagnosed with leprosy, was performed by ELISA after elution of bloodspots from filter paper. RESULTS: Anti-PGL-I Ab levels at intake did not significantly differ between contacts who developed leprosy during the study and those who remained free of disease. Moreover, anti-PGL-I serology was not prognostic in this population as no significant correlation was identified between anti-PGL-I Ab levels at intake and the onset of leprosy. CONCLUSION: In this highly endemic population in Bangladesh, no association was observed between anti-PGL-I Ab levels and onset of disease, urging the need for an extended, more specific biomarker signature for early detection of leprosy in this area. TRIAL REGISTRATION: ClinicalTrials.gov ISRCTN61223447.
[Mh] Termos MeSH primário: Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/imunologia
Glicolipídeos/imunologia
Hanseníase/diagnóstico
Mycobacterium leprae/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Bangladesh/epidemiologia
Biomarcadores/sangue
Criança
Pré-Escolar
Estudos de Coortes
Diagnóstico Tardio/prevenção & controle
Feminino
Seres Humanos
Imunoglobulina G/sangue
Imunoglobulina M/sangue
Lactente
Hanseníase/imunologia
Hanseníase/transmissão
Estudos Longitudinais
Masculino
Mycobacterium leprae/isolamento & purificação
Estudos Prospectivos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Bacterial); 0 (Biomarkers); 0 (Glycolipids); 0 (Immunoglobulin G); 0 (Immunoglobulin M); 0 (phenolic glycolipid I, Mycobacterium leprae)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006083


  4 / 5143 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29253897
[Au] Autor:Negera E; Walker SL; Bekele Y; Dockrell HM; Lockwood DN
[Ad] Endereço:London School of Hygiene and Tropical Medicine (LSHTM), Faculty of Infectious Tropical Diseases, London, United Kingdom.
[Ti] Título:Increased activated memory B-cells in the peripheral blood of patients with erythema nodosum leprosum reactions.
[So] Source:PLoS Negl Trop Dis;11(12):e0006121, 2017 12.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:B-cells, in addition to antibody secretion, have emerged increasingly as effector and immunoregulatory cells in several chronic inflammatory diseases. Although Erythema Nodosum Leprosum (ENL) is an inflammatory complication of leprosy, the role of B- cell subsets has never been studied in this patient group. Therefore, it would be interesting to examine the contribution of B-cells in the pathogenesis of ENL. A case-control study design was used to recruit 30 untreated patients with ENL and 30 non-reactional lepromatous leprosy (LL) patient controls at ALERT Hospital, Ethiopia. Peripheral blood samples were obtained before, during and after treatment from each patient. Peripheral blood mononuclear cells (PBMCs) were isolated and used for immunophenotyping of B- cell subsets by flow cytometry. The kinetics of B-cells in patients with ENL before, during and after Prednisolone treatment of ENL was compared with LL patient controls as well as within ENL group. Total B-cells, mature B-cells and resting memory B-cells were not significantly different between patients with ENL reactions and LL controls before treatment. Interestingly, while the percentage of naive B-cells was significantly lower in untreated ENL patients than in LL patient controls, the percentage of activated memory B-cells was significantly higher in these untreated ENL patients than in LL controls. On the other hand, the percentage of tissue-like memory B-cells was considerably low in untreated ENL patients compared to LL controls. It appears that the lower frequency of tissue-like memory B-cells in untreated ENL could promote the B-cell/T-cell interaction in these patients through downregulation of inhibitory molecules unlike in LL patients. Conversely, the increased production of activated memory B-cells in ENL patients could imply the scale up of immune activation through antigen presentation to T-cells. However, the generation and differential function of these memory B-cells need further investigation. The finding of increased percentage of activated memory B-cells in untreated patients with ENL reactions suggests the association of these cells with the ENL pathology. The mechanism by which inflammatory reactions like ENL affecting these memory cells and contributing to the disease pathology is an interesting area to be explored for and could lead to the development of novel and highly efficacious drug for ENL treatment.
[Mh] Termos MeSH primário: Anticorpos Antibacterianos/imunologia
Linfócitos B/imunologia
Eritema Nodoso/imunologia
Eritema Nodoso/patologia
Hanseníase/patologia
Mycobacterium leprae/imunologia
[Mh] Termos MeSH secundário: Anti-Inflamatórios/uso terapêutico
Estudos de Casos e Controles
Eritema Nodoso/tratamento farmacológico
Etiópia
Seres Humanos
Memória Imunológica/imunologia
Hanseníase/imunologia
Hanseníase/microbiologia
Contagem de Linfócitos
Prednisolona/uso terapêutico
Linfócitos T/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antibodies, Bacterial); 9PHQ9Y1OLM (Prednisolone)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006121


  5 / 5143 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29208413
[Au] Autor:Kaur G; Sharma A; Narang T; Dogra S; Kaur J
[Ad] Endereço:Department of Biotechnology, BMS Block-1, South Campus, Panjab University, Chandigarh, 160014, India.
[Ti] Título:Characterization of ML0314c of Mycobacterium leprae and deciphering its role in the immune response in leprosy patients.
[So] Source:Gene;643:26-34, 2018 Feb 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mycobacterium leprae has a reduced genome size due to the reductive evolution over a long period of time. Lipid metabolism plays an important role in the life cycle and pathogenesis of this bacterium. In comparison to 26 lip genes (Lip A-Z) of M. tuberculosis, M. leprae retained only three orthologs indicating their importance in its life cycle. ML0314c (LipU) is one of them. It is conserved throughout the mycobacterium species. Bioinformatics analysis showed the presence of an α/ß hydrolase fold and 'GXSXG' characteristic of the esterases/lipases. The gene was expressed in E. coli and purified to homogeneity. It showed preference towards short chain esters with pNP-acetate as the preferred substrate. The enzyme showed optimal activity at 45°C and pH8.0. ML0314c protein was stable between temperatures ranging from 20 to 60°C and pH5.0-8.0, i.e., relatively acidic and neutral conditions. The active site residues predicted bioinformatically were confirmed to be Ser168, Glu267, and His297 by site directed mutagenesis. E-serine, DEPC and Tetrahydrolipstatin (THL) completely inhibited the activity of ML0314c. The protein was localized in cell wall and extracellular medium. Several antigenic epitopes were predicted in ML0314c. Protein elicited strong humoral immune response in leprosy patients, whereas, a reduced immune response was observed in the relapsed cases. No humoral response was observed in treatment completed patients. Overexpression of ml0314c in the surrogate host M. smegmatis showed marked difference in the colony morphology and growth rate. In conclusion, ML0314c is a secretary carboxyl esterase that could modulate the immune response in leprosy patients.
[Mh] Termos MeSH primário: Lipólise/genética
Mycobacterium leprae/genética
Mycobacterium leprae/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos/genética
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Hidrolases de Éster Carboxílico/metabolismo
Domínio Catalítico/genética
Clonagem Molecular/métodos
Escherichia coli/genética
Seres Humanos
Concentração de Íons de Hidrogênio
Hanseníase/metabolismo
Hanseníase/microbiologia
Lipase/genética
Metabolismo dos Lipídeos/genética
Lipídeos
Mutagênese Sítio-Dirigida/métodos
Mycobacterium tuberculosis/genética
Especificidade por Substrato/genética
Fatores de Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Lipids); 0 (Virulence Factors); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.- (LipY protein, Mycobacterium tuberculosis); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  6 / 5143 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:29211246
[Au] Autor:Lima FR; Takenami I; Cavalcanti MA; Riley LW; Arruda S
[Ad] Endereço:Fundação Oswaldo Cruz-Fiocruz, Centro de Pesquisas Gonçalo Moniz, Laboratório Avançado de Saúde Pública, Salvador, BA, Brasil.
[Ti] Título:ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy.
[So] Source:Mem Inst Oswaldo Cruz;112(12):844-849, 2017 Dec.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Leprosy is a chronic infectious disease caused by the obligate intracellular bacillus Mycobacterium leprae. Because leprosy diagnosis is complex and requires professional expertise, new tools and methodologies are needed to detect cases in early stages and prevent transmission. The M. leprae genome contains mce1A, which encodes a putative mammalian cell entry protein (Mce1A). We hypothesised that the presence of Mce1A on the cell surface could be detected by the host's immune system. OBJECTIVE: The aim of this study was to evaluate antibody responses against the Mce1A protein in leprosy patients, household contacts of patients, and the general population to present an addition tool for leprosy diagnosis. METHODS: A cross-sectional study involving 89 volunteers [55 leprosy cases, 12 household contacts (HHC) and 22 endemic controls (EC)] was conducted at Couto Maia Hospital, in Salvador, Bahia (BA), Brazil. RESULTS: The median anti-Mce1A IgA was significantly higher in multibacillary (MB) and paucibacillary (PB) cases than in EC (p < 0.0001). A similar trend was observed in IgM levels, which were significantly higher in both MB (p < 0.0001) and PB (p = 0.0006) groups compared to in EC individuals. The greatest differences were observed for IgG class-specific antibodies against Mce1A. The median levels of MB and PB were significantly higher compared to both controls HHC and EC (MB or PB vs EC, MB vs HHC p < 0.0001; PB vs HHC, p = 0.0013). Among leprosy cases, IgG enzyme-linked immunosorbent assay sensitivity and specificity were 92.7% and 97.1%, respectively. IgG positivity was confirmed in 92.1% and 94.1% of MB and PB patients, respectively. CONCLUSION: This novel diagnostic approach presents an easy, non-invasive, and inexpensive method for leprosy screening, which may be applicable in endemic areas.
[Mh] Termos MeSH primário: Anticorpos Antibacterianos/sangue
Proteínas de Bactérias/imunologia
Imunoglobulina G/sangue
Hanseníase/diagnóstico
Mycobacterium leprae/imunologia
[Mh] Termos MeSH secundário: Adulto
Estudos de Casos e Controles
Ensaio de Imunoadsorção Enzimática
Características da Família
Feminino
Seres Humanos
Masculino
Meia-Idade
Sensibilidade e Especificidade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Bacterial Proteins); 0 (Immunoglobulin G); 0 (Mce1A protein, Mycobacterium leprae)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  7 / 5143 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:29211243
[Au] Autor:Bernardes F; Paula NA; Leite MN; Abi-Rached TLC; Vernal S; Silva MBD; Barreto JG; Spencer JS; Frade MAC
[Ad] Endereço:Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Departamento de Clínica Médica, Divisão de Dermatologia, Ribeirão Preto, SP, Brasil.
[Ti] Título:Evidence of hidden leprosy in a supposedly low endemic area of Brazil.
[So] Source:Mem Inst Oswaldo Cruz;112(12):822-828, 2017 Dec.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Show that hidden endemic leprosy exists in a municipality of inner São Paulo state (Brazil) with active surveillance actions based on clinical and immunological evaluations. METHODS: The study sample was composed by people randomly selected by a dermatologist during medical care in the public emergency department and by active surveillance carried out during two days at a mobile clinic. All subjects received a dermato-neurological examination and blood sampling to determine anti-PGL-I antibody titers by enzyme-linked immunosorbent assay (ELISA). RESULTS: From July to December 2015, 24 new cases of leprosy were diagnosed; all were classified as multibacillary (MB) leprosy, one with severe Lucio's phenomenon. Seventeen (75%) were found with grade-1 or 2 disability at the moment of diagnosis. Anti-PGL-I titer was positive in 31/133 (23.3%) individuals, only 6/24 (25%) were positive in newly diagnosed leprosy cases. CONCLUSIONS: During the last ten years before this study, the average new case detection rate (NCDR) in this town was 2.62/100,000 population. After our work, the NCDR was raised to 42.8/100,000. These results indicate a very high number of hidden leprosy cases in this supposedly low endemic area of Brazil.
[Mh] Termos MeSH primário: Anticorpos Antibacterianos/sangue
Hanseníase/epidemiologia
Mycobacterium leprae/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Brasil/epidemiologia
Doenças Endêmicas
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Hanseníase/diagnóstico
Masculino
Programas de Rastreamento
Meia-Idade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  8 / 5143 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:29211240
[Au] Autor:Arraes MLBM; Holanda MV; Lima LNGC; Sabadia JAB; Duarte CR; Almeida RLF; Kendall C; Kerr LRS; Frota CC
[Ad] Endereço:Universidade Federal do Ceará, Faculdade de Medicina, Departamento de Patologia e Medicina Legal, Fortaleza, CE, Brasil.
[Ti] Título:Natural environmental water sources in endemic regions of northeastern Brazil are potential reservoirs of viable Mycobacterium leprae.
[So] Source:Mem Inst Oswaldo Cruz;112(12):805-811, 2017 Dec.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The detection of live Mycobacterium leprae in soil and animals other than humans suggests that the environment plays a role in the transmission of leprosy. OBJECTIVE: The objective of this study was to investigate the presence of viable M. leprae in natural water sources used by the local population in five municipalities in the state of Ceará, northeastern Brazil. METHODS: Samples were collected from 30 different sources. Viable bacilli were identified by reverse transcriptase polymerase chain reaction (PCR) of the M. leprae gyrA gene and sequencing of the PCR products. Physicochemical properties of each water source were also assessed. FINDINGS: M. leprae gyrA mRNA was found in 23 (76.7%) of the water sources. No association was found between depth of the water and sample positivity, nor was there any association between the type of water used by the population and sample positivity. An association between viable M. leprae and temperature and pH was found. Georeferencing showed a relation between the residences of leprosy cases and water source containing the bacterium. MAIN CONCLUSIONS: The finding of viable M. leprae in natural water sources associated with human contact suggests that the environment plays an important role in maintaining endemic leprosy in the study region.
[Mh] Termos MeSH primário: Mycobacterium leprae/isolamento & purificação
Microbiologia da Água
[Mh] Termos MeSH secundário: Brasil
DNA Bacteriano/genética
Reservatórios de Doenças
Genótipo
Mycobacterium leprae/genética
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  9 / 5143 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29176796
[Au] Autor:Santos DFD; Mendonça MR; Antunes DE; Sabino EFP; Pereira RC; Goulart LR; Goulart IMB
[Ad] Endereço:National Reference Center for Sanitary Dermatology and Leprosy, Clinics' Hospital, School of Medicine, Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil.
[Ti] Título:Revisiting primary neural leprosy: Clinical, serological, molecular, and neurophysiological aspects.
[So] Source:PLoS Negl Trop Dis;11(11):e0006086, 2017 Nov.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Leprosy neuropathy is considered the most common peripheral neuropathy of infectious etiology worldwide, representing a public health problem. Clinical diagnosis of primary neural leprosy (PNL) is challenging, since no skin lesions are found and the slit skin smear bacilloscopy is negative. However, there are still controversial concepts regarding the primary-neural versus pure-neural leprosy definition, which will be explored by using multiple clinical-laboratory analyses in this study. METHODOLOGY/PRINCIPAL FINDINGS: Seventy patients diagnosed with primary neural leprosy from 2014 to 2016 underwent clinical, laboratorial and neurophysiological evaluation. All patients presented an asymmetric neural impairment, with nerve thickening in 58.6%. Electroneuromyography showed a pattern of mononeuropathy in 51.4%. Positivity for ELISA anti-PGL1 was 52.9%, while the qPCR of slit skin smear was 78.6%. The qPCR of nerve biopsies was positive in 60.8%. Patients with multiple mononeuropathy patterns showed lower levels of anti-PGL-1 (p = 0.0006), and higher frequency of neural thickening (p = 0.0008) and sensory symptoms (p = 0.01) than those with mononeuropathy. CONCLUSIONS/SIGNIFICANCE: PNL is not a synonym of pure neural leprosy, as this condition may include a generalized immune response and also a skin involvement, documented by molecular findings. Immunological, molecular, and neurophysiological tools must be implemented for diagnosing primary neural leprosy to achieve effective treatment and reduction of its resultant disabilities that still represent a public health problem in several developing nations. Finally, we propose a algorithm and recommendations for the diagnosis of primary neural leprosy based on the combination of the three clinical-laboratorial tools.
[Mh] Termos MeSH primário: Hanseníase Tuberculoide/patologia
Doenças do Sistema Nervoso Periférico/patologia
[Mh] Termos MeSH secundário: Adulto
Algoritmos
Brasil
Feminino
Seres Humanos
Hanseníase Tuberculoide/complicações
Hanseníase Tuberculoide/diagnóstico
Masculino
Meia-Idade
Mycobacterium leprae
Nervos Periféricos/patologia
Doenças do Sistema Nervoso Periférico/diagnóstico
Doenças do Sistema Nervoso Periférico/etiologia
Reação em Cadeia da Polimerase em Tempo Real
Pele/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006086


  10 / 5143 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29031287
[Au] Autor:Soto LA; Caballero N; Fuentes LR; Muñoz PT; Gómez Echevarría JR; López MP; Bornay Llinares FJ; Stanford JL; Stanford CA; Donoghue HD
[Ad] Endereço:Universidad Miguel Hernández de Elche, Alicante, Spain.
[Ti] Título:Leprosy Associated with Atypical Cutaneous Leishmaniasis in Nicaragua and Honduras.
[So] Source:Am J Trop Med Hyg;97(4):1103-1110, 2017 Oct.
[Is] ISSN:1476-1645
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Central America, few cases of leprosy have been reported, but the disease may be unrecognized. Diagnosis is based on clinical criteria and histology. Preliminary field work in Nicaragua and Honduras found patients, including many children, with skin lesions clinically suggestive of atypical cutaneous leishmaniasis or indeterminate leprosy. Histology could not distinguish these diseases although acid-fast organisms were visible in a few biopsies. Lesions healed after standard antimicrobial therapy for leprosy. In the present study, patients, family members, and other community members were skin-tested and provided nasal swabs and blood samples. Biopsies were taken from a subgroup of patients with clinical signs of infection. Two laboratories analyzed samples, using local in-house techniques. , spp. and were detected using polymerase chain reactions. DNA was detected in blood samples and nasal swabs, including some cases where leprosy was not clinically suspected. spp. were also detected in blood and nasal swabs. Most biopsies contained DNA and coinfection of spp. with occurred in 33% of cases. DNA was also detected and sequenced from Nicaraguan and Honduran environmental samples. In conclusion, leprosy and leishmaniasis are present in both regions, and leprosy appears to be widespread. The nature of any relationship between these two pathogens and the epidemiology of these infections need to be elucidated.
[Mh] Termos MeSH primário: Leishmania/isolamento & purificação
Leishmaniose Cutânea/diagnóstico
Leishmaniose Cutânea/epidemiologia
Hanseníase/diagnóstico
Hanseníase/epidemiologia
Mycobacterium leprae/isolamento & purificação
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Honduras/epidemiologia
Seres Humanos
Masculino
Meia-Idade
Nicarágua/epidemiologia
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.16-0622



página 1 de 515 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde