[PMID]: | 28743081 |
[Au] Autor: | Yu Z; Zhang C; Zhou M; Li Q; Li H; Duan W; Li X; Feng Y; Xie J |
[Ad] Endereço: | Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715 |
[Ti] Título: | Mycobacterium tuberculosis PPE44 (Rv2770c) is involved in response to multiple stresses and promotes the macrophage expression of IL-12 p40 and IL-6 via the p38, ERK, and NF-κB signaling axis. |
[So] Source: | Int Immunopharmacol;50:319-329, 2017 Sep. |
[Is] ISSN: | 1878-1705 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | Tuberculosis (TB), caused by Mycobacterium tuberculosis, remains a formidable threat to global public health. The successful intracellular persistence of M. tuberculosis significantly contributes to the intractability of tuberculosis. Proline-glutamic acid (PE) and proline-proline-glutamic acid (PPE) are mycobacterial exclusive protein families that widely reported to be involved in the bacterial virulence, physiology and interaction with host. Rv2770c (PPE44), a predicted virulence factor, was up-regulated upon the infected guinea pig lungs. To investigate the role of Rv2770c, we heterologously expressed the PPE44 in the nonpathogenic fast-growing M. smegmatis strain. Subcellular location analysis demonstrated that Rv2770c is a cell wall associated protein, suggestive of a potential candidate involved in host-pathogen interaction. The Rv2770c can enhance M. smegmatis survival within macrophages and under stresses such as H O , SDS, diamide exposure, and low pH condition. M. smegmatis expressing Rv2770c is more virulent as testified by the increased death of macrophages and the increased expression of interlukin-6 (IL-6) and interlukin-12p40 (IL-12p40). Moreover, Rv2770c altered the secretion of IL-6 and IL-12p40 of macrophages via NF-κB, ERK1/2 and p38 MAPK axis. Taken together, this study implicated that Rv2770c was a virulent factor actively engaged in the interaction with host macrophage. |
[Mh] Termos MeSH primário: |
Antígenos de Bactérias/metabolismo Pulmão/patologia Macrófagos/imunologia Infecções por Micobactéria não Tuberculosa/imunologia Mycobacterium smegmatis/genética Mycobacterium tuberculosis/imunologia Tuberculose/imunologia Fatores de Virulência/metabolismo
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[Mh] Termos MeSH secundário: |
Animais Antígenos de Bactérias/genética Dano ao DNA MAP Quinases Reguladas por Sinal Extracelular/metabolismo Cobaias Interações Hospedeiro-Patógeno Seres Humanos Subunidade p40 da Interleucina-12/metabolismo Interleucina-6/metabolismo Pulmão/microbiologia Sistema de Sinalização das MAP Quinases Macrófagos/microbiologia Mycobacterium tuberculosis/genética NF-kappa B/metabolismo Transdução de Sinais Células THP-1
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antigens, Bacterial); 0 (Interleukin-12 Subunit p40); 0 (Interleukin-6); 0 (NF-kappa B); 0 (PPE44 protein, Mycobacterium tuberculosis); 0 (Virulence Factors); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases) |
[Em] Mês de entrada: | 1802 |
[Cu] Atualização por classe: | 180221 |
[Lr] Data última revisão:
| 180221 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170726 |
[St] Status: | MEDLINE |
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