Base de dados : MEDLINE
Pesquisa : B03.510.024.400 [Categoria DeCS]
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  1 / 29 MEDLINE  
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[PMID]:28549981
[Au] Autor:Stout MJ; Zhou Y; Wylie KM; Tarr PI; Macones GA; Tuuli MG
[Ad] Endereço:Department of Obstetrics and Gynecology, Washington University in St Louis School of Medicine, St. Louis, MO. Electronic address: stoutm@wudosis.wustl.edu.
[Ti] Título:Early pregnancy vaginal microbiome trends and preterm birth.
[So] Source:Am J Obstet Gynecol;217(3):356.e1-356.e18, 2017 Sep.
[Is] ISSN:1097-6868
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Despite decades of attempts to link infectious agents to preterm birth, an exact causative microbe or community of microbes remains elusive. Nonculture 16S ribosomal RNA gene sequencing suggests important racial differences and pregnancy specific changes in the vaginal microbial communities. A recent study examining the association of the vaginal microbiome and preterm birth documented important findings but was performed in a predominantly white cohort. Given the important racial differences in bacterial communities within the vagina as well as persistent racial disparities in preterm birth, it is important to examine cohorts with varied demographic compositions. OBJECTIVE: To characterize vaginal microbial community characteristics in a large, predominantly African-American, longitudinal cohort of pregnant women and test whether particular vaginal microbial community characteristics are associated with the risk for subsequent preterm birth. STUDY DESIGN: This is a nested case-control study within a prospective cohort study of women with singleton pregnancies, not on supplemental progesterone, and without cervical cerclage in situ. Serial mid-vaginal swabs were obtained by speculum exam at their routine prenatal visits. Sequencing of the V1V3 region of the 16S rRNA gene was performed on the Roche 454 platform. Alpha diversity community characteristics including richness, Shannon diversity, and evenness as well as beta diversity metrics including Bray Curtis Dissimilarity and specific taxon abundance were compared longitudinally in women who delivered preterm to those who delivered at term. RESULTS: A total of 77 subjects contributed 149 vaginal swabs longitudinally across pregnancy. Participants were predominantly African-American (69%) and had a preterm birth rate of 31%. In subjects with subsequent term delivery, the vaginal microbiome demonstrated stable community richness and Shannon diversity, whereas subjects with subsequent preterm delivery had significantly decreased vaginal richness, diversity, and evenness during pregnancy (P < .01). This change occurred between the first and second trimesters. Within-subject comparisons across pregnancy showed that preterm birth is associated with increased vaginal microbiome instability compared to term birth. No distinct taxa were associated with preterm birth. CONCLUSION: In a predominantly African-American population, a significant decrease of vaginal microbial community richness and diversity is associated with preterm birth. The timing of this suppression appears early in pregnancy, between the first and second trimesters, suggesting that early gestation may be an ecologically important time for events that ordain subsequent term and preterm birth outcomes.
[Mh] Termos MeSH primário: Microbiota
Nascimento Prematuro
Vagina/microbiologia
[Mh] Termos MeSH secundário: Afroamericanos
Estudos de Casos e Controles
Feminino
Gardnerella/genética
Idade Gestacional
Seres Humanos
Lactobacillus/genética
Gravidez
Trimestres da Gravidez
Estudos Prospectivos
RNA Ribossômico 16S
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170528
[St] Status:MEDLINE


  2 / 29 MEDLINE  
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[PMID]:27861551
[Au] Autor:Malki K; Shapiro JW; Price TK; Hilt EE; Thomas-White K; Sircar T; Rosenfeld AB; Kuffel G; Zilliox MJ; Wolfe AJ; Putonti C
[Ad] Endereço:Department of Biology, Loyola University Chicago, Chicago, Illinois, United States of America.
[Ti] Título:Genomes of Gardnerella Strains Reveal an Abundance of Prophages within the Bladder Microbiome.
[So] Source:PLoS One;11(11):e0166757, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial surveys of the vaginal and bladder human microbiota have revealed an abundance of many similar bacterial taxa. As the bladder was once thought to be sterile, the complex interactions between microbes within the bladder have yet to be characterized. To initiate this process, we have begun sequencing isolates, including the clinically relevant genus Gardnerella. Herein, we present the genomic sequences of four Gardnerella strains isolated from the bladders of women with symptoms of urgency urinary incontinence; these are the first Gardnerella genomes produced from this niche. Congruent to genomic characterization of Gardnerella isolates from the reproductive tract, isolates from the bladder reveal a large pangenome, as well as evidence of high frequency horizontal gene transfer. Prophage gene sequences were found to be abundant amongst the strains isolated from the bladder, as well as amongst publicly available Gardnerella genomes from the vagina and endometrium, motivating an in depth examination of these sequences. Amongst the 39 Gardnerella strains examined here, there were more than 400 annotated prophage gene sequences that we could cluster into 95 homologous groups; 49 of these groups were unique to a single strain. While many of these prophages exhibited no sequence similarity to any lytic phage genome, estimation of the rate of phage acquisition suggests both vertical and horizontal acquisition. Furthermore, bioinformatic evidence indicates that prophage acquisition is ongoing within both vaginal and bladder Gardnerella populations. The abundance of prophage sequences within the strains examined here suggests that phages could play an important role in the species' evolutionary history and in its interactions within the complex communities found in the female urinary and reproductive tracts.
[Mh] Termos MeSH primário: Gardnerella/genética
Genoma Bacteriano
Microbiota
Prófagos/genética
Bexiga Urinária/microbiologia
[Mh] Termos MeSH secundário: Adulto
Biologia Computacional/métodos
Elementos de DNA Transponíveis
Feminino
Gardnerella/virologia
Genes Virais
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Fases de Leitura Aberta
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166757


  3 / 29 MEDLINE  
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[PMID]:27428605
[Au] Autor:Stockdale CK
[Ad] Endereço:Department of Obstetrics and Gynecology, University of Iowa, Iowa City, IA.
[Ti] Título:A Positive Culture Result for Gardnerella Is Not Diagnostic of Bacterial Vaginosis.
[So] Source:J Low Genit Tract Dis;20(4):281-2, 2016 Oct.
[Is] ISSN:1526-0976
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Gardnerella/isolamento & purificação
Vaginose Bacteriana/diagnóstico
[Mh] Termos MeSH secundário: Técnicas Bacteriológicas/métodos
Medicina Clínica/métodos
Feminino
Seres Humanos
[Pt] Tipo de publicação:EDITORIAL
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160719
[St] Status:MEDLINE
[do] DOI:10.1097/LGT.0000000000000237


  4 / 29 MEDLINE  
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[PMID]:26266808
[Au] Autor:Albert AY; Chaban B; Wagner EC; Schellenberg JJ; Links MG; van Schalkwyk J; Reid G; Hemmingsen SM; Hill JE; Money D; VOGUE Research Group
[Ad] Endereço:Women's Health Research Institute, Vancouver, BC, Canada.
[Ti] Título:A Study of the Vaginal Microbiome in Healthy Canadian Women Utilizing cpn60-Based Molecular Profiling Reveals Distinct Gardnerella Subgroup Community State Types.
[So] Source:PLoS One;10(8):e0135620, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The vaginal microbiota is important in women's reproductive and overall health. However, the relationships between the structure, function and dynamics of this complex microbial community and health outcomes remain elusive. The objective of this study was to determine the phylogenetic range and abundance of prokaryotes in the vaginal microbiota of healthy, non-pregnant, ethnically diverse, reproductive-aged Canadian women. Socio-demographic, behavioural and clinical data were collected and vaginal swabs were analyzed from 310 women. Detailed profiles of their vaginal microbiomes were generated by pyrosequencing of the chaperonin-60 universal target. Six community state types (CST) were delineated by hierarchical clustering, including three Lactobacillus-dominated CST (L. crispatus, L. iners, L. jensenii), two Gardnerella-dominated (subgroups A and C) and an "intermediate" CST which included a small number of women with microbiomes dominated by seven other species or with no dominant species but minority populations of Streptococcus, Staphylococcus, Peptoniphilus, E. coli and various Proteobacteria in co-dominant communities. The striking correspondence between Nugent score and deep sequencing CST continues to reinforce the basic premise provided by the simpler Gram stain method, while additional analyses reveal detailed cpn60-based phylogeny and estimated abundance in microbial communities from vaginal samples. Ethnicity was the only demographic or clinical characteristic predicting CST, with differences in Asian and White women (p = 0.05). In conclusion, this study confirms previous work describing four cpn60-based subgroups of Gardnerella, revealing previously undescribed CST. The data describe the range of bacterial communities seen in Canadian women presenting with no specific vaginal health concerns, and provides an important baseline for future investigations of clinically important cohorts.
[Mh] Termos MeSH primário: Gardnerella/genética
Vagina/microbiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Canadá
Feminino
Gardnerella/classificação
Seres Humanos
Microbiota/genética
Meia-Idade
Filogenia
Saúde da Mulher
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150813
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0135620


  5 / 29 MEDLINE  
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[PMID]:26210757
[Au] Autor:Pearce MM; Zilliox MJ; Rosenfeld AB; Thomas-White KJ; Richter HE; Nager CW; Visco AG; Nygaard IE; Barber MD; Schaffer J; Moalli P; Sung VW; Smith AL; Rogers R; Nolen TL; Wallace D; Meikle SF; Gai X; Wolfe AJ; Brubaker L; Pelvic Floor Disorders Network
[Ad] Endereço:Departments of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL.
[Ti] Título:The female urinary microbiome in urgency urinary incontinence.
[So] Source:Am J Obstet Gynecol;213(3):347.e1-11, 2015 Sep.
[Is] ISSN:1097-6868
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The purpose of this study was to characterize the urinary microbiota in women who are planning treatment for urgency urinary incontinence and to describe clinical associations with urinary symptoms, urinary tract infection, and treatment outcomes. STUDY DESIGN: Catheterized urine samples were collected from multisite randomized trial participants who had no clinical evidence of urinary tract infection; 16S ribosomal RNA gene sequencing was used to dichotomize participants as either DNA sequence-positive or sequence-negative. Associations with demographics, urinary symptoms, urinary tract infection risk, and treatment outcomes were determined. In sequence-positive samples, microbiotas were characterized on the basis of their dominant microorganisms. RESULTS: More than one-half (51.1%; 93/182) of the participants' urine samples were sequence-positive. Sequence-positive participants were younger (55.8 vs 61.3 years old; P = .0007), had a higher body mass index (33.7 vs 30.1 kg/m(2); P = .0009), had a higher mean baseline daily urgency urinary incontinence episodes (5.7 vs 4.2 episodes; P < .0001), responded better to treatment (decrease in urgency urinary incontinence episodes, -4.4 vs -3.3; P = .0013), and were less likely to experience urinary tract infection (9% vs 27%; P = .0011). In sequence-positive samples, 8 major bacterial clusters were identified; 7 clusters were dominated not only by a single genus, most commonly Lactobacillus (45%) or Gardnerella (17%), but also by other taxa (25%). The remaining cluster had no dominant genus (13%). CONCLUSION: DNA sequencing confirmed urinary bacterial DNA in many women with urgency urinary incontinence who had no signs of infection. Sequence status was associated with baseline urgency urinary incontinence episodes, treatment response, and posttreatment urinary tract infection risk.
[Mh] Termos MeSH primário: Bacteriúria/microbiologia
Infecções por Bacteroidaceae/microbiologia
Infecções por Bactérias Gram-Positivas/microbiologia
Microbiota/genética
RNA Ribossômico 16S/análise
Incontinência Urinária de Urgência/microbiologia
Sistema Urinário/microbiologia
[Mh] Termos MeSH secundário: Inibidores da Liberação da Acetilcolina/uso terapêutico
Adulto
Fatores Etários
Idoso
Bacteriúria/epidemiologia
Infecções por Bacteroidaceae/epidemiologia
Índice de Massa Corporal
Toxinas Botulínicas Tipo A/uso terapêutico
Antagonistas Colinérgicos/uso terapêutico
Feminino
Gardnerella/genética
Gardnerella/isolamento & purificação
Infecções por Bactérias Gram-Positivas/epidemiologia
Seres Humanos
Lactobacillus/genética
Lactobacillus/isolamento & purificação
Meia-Idade
Obesidade/epidemiologia
Prevotella/genética
Prevotella/isolamento & purificação
Qualidade de Vida
Resultado do Tratamento
Incontinência Urinária de Urgência/epidemiologia
Incontinência Urinária de Urgência/terapia
Infecções Urinárias/epidemiologia
Infecções Urinárias/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Acetylcholine Release Inhibitors); 0 (Cholinergic Antagonists); 0 (RNA, Ribosomal, 16S); E211KPY694 (onabotulinumtoxinA); EC 3.4.24.69 (Botulinum Toxins, Type A)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:150727
[St] Status:MEDLINE


  6 / 29 MEDLINE  
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[PMID]:25205755
[Au] Autor:Khan KN; Fujishita A; Kitajima M; Hiraki K; Nakashima M; Masuzaki H
[Ad] Endereço:Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Nagasaki University, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan nemokhan@nagasaki-u.ac.jp.
[Ti] Título:Intra-uterine microbial colonization and occurrence of endometritis in women with endometriosis†.
[So] Source:Hum Reprod;29(11):2446-56, 2014 Nov.
[Is] ISSN:1460-2350
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:STUDY QUESTION: Is there any risk of intra-uterine bacterial colonization and concurrent occurrence of endometritis in women with endometriosis? SUMMARY ANSWER: An increase in intra-uterine microbial colonization and concurrent endometritis occurred in women with endometriosis that was further increased after GnRH agonist (GnRHa) treatment. WHAT IS KNOWN ALREADY: Higher bacterial contamination of menstrual blood and increased endotoxin level in menstrual and peritoneal fluids have been found in women with endometriosis than in control women. However, information on intra-uterine microbial colonization across the phases of the menstrual cycle and possible occurrence of endometritis in women with endometriosis is still lacking. STUDY DESIGN, SIZE AND DURATION: This is a case-controlled study with prospective collection of vaginal smears/endometrial samples from women with and without endometriosis and retrospective evaluation. PARTICIPANTS/MATERIALS, SETTING, METHODS: Vaginal smears and endometrial smears were collected from 73 women with endometriosis and 55 control women. Twenty of the women with endometriosis and 19 controls had received GnRHa therapy for a period of 4-6 months. Vaginal pH was measured by intra-vaginal insertion of a pH paper strip. The bacterial vaginosis (BV) score was analyzed by Gram-staining of vaginal smears and based on a modified Nugent-BV scoring system. A panel of bacteria was analyzed by culture of endometrial samples from women treated with GnRHa or not treated. Immunohistochemcial analysis was performed using antibody against Syndecan-1 (CD138) and myeloperoxidase in endometrial biopsy specimens from women with and without endometriosis. MAIN RESULTS AND THE ROLE OF CHANCE: A significant shifting of intra-vaginal pH to ≥4.5 was observed in women with endometriosis compared with control women (79.3 versus 58.4%, P < 0.03). Compared with untreated women, use of GnRHa therapy also shifted vaginal pH to ≥4.5 in both control women (P = 0.004) and in women with endometriosis (P = 0.03). A higher risk of increasing intermediate flora (total score, 4-6) (P = 0.05) was observed in women with endometriosis who had GnRHa treatment versus untreated women. The number of colony forming units (CFU/ml) of Gardnerella, α-Streptococcus, Enterococci and Escherichia coli was significantly higher in endometrial samples from women with endometriosis than control women (P < 0.05 for each bacteria). GnRHa-treated women also showed significantly higher colony formation for some of these bacteria in endometrial samples than in untreated women (Gardnerella and E. coli for controls; Gardnerella, Enterococci and E. coli for women with endometriosis, P < 0.05 for all). Although there was no significant difference in the occurrence of acute endometritis between women with and without endometriosis, both GnRHa-treated controls and women with endometriosis had a significantly higher occurrence of acute endometritis (P = 0.003 for controls, P = 0.001 for endometriosis versus untreated women). Multiple analysis of covariance analysis revealed that an intra-vaginal pH of ≥4.5 (P = 0.03) and use of GnRHa (P = 0.04) were potential factors that were significantly and independently associated with intra-uterine microbial colonization and occurrence of endometritis in women with endometriosis. These findings indicated the occurrence of sub-clinical uterine infection and endometritis in women with endometriosis after GnRHa treatment. LIMITATIONS, REASONS FOR CAUTION: We cannot exclude the introduction of bias from unknown previous treatment with immunosuppressing or anti-microbial agents. We have studied a limited range of bacterial species and used only culture-based methods. More sensitive molecular approaches would further delineate the similarities/differences between the vaginal cavity and uterine environment. WIDER IMPLICATIONS OF THE FINDINGS: Our current findings may have epidemiological and biological implications and help in understanding the pathogenesis of endometriosis and related disease burden. The worsening of intra-uterine microbial colonization and higher occurrence of endometritis in women with endometriosis who were treated with GnRHa identifies some future therapeutic avenues for the management, as well as prevention of recurrence, of endometriosis. Further studies are needed to examine intra-uterine colonization of a broad range of common bacteria as well as different viruses and their role in the occurrence of endometritis. STUDY FUNDING/COMPETING INTERESTS: This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Sports, Culture, Science and Technology of Japan. There is no conflict of interest related to this study. TRIAL REGISTRATION NUMBER: Not applicable.
[Mh] Termos MeSH primário: Endometriose/complicações
Endometrite/complicações
Escherichia coli/isolamento & purificação
Gardnerella/isolamento & purificação
Streptococcus/isolamento & purificação
Útero/microbiologia
[Mh] Termos MeSH secundário: Adulto
Estudos de Casos e Controles
Contagem de Colônia Microbiana
Endometriose/microbiologia
Endometriose/patologia
Endometrite/microbiologia
Endometrite/patologia
Feminino
Seres Humanos
Estudos Prospectivos
Útero/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1506
[Cu] Atualização por classe:141010
[Lr] Data última revisão:
141010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140911
[St] Status:MEDLINE
[do] DOI:10.1093/humrep/deu222


  7 / 29 MEDLINE  
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[PMID]:25006228
[Au] Autor:Pearce MM; Hilt EE; Rosenfeld AB; Zilliox MJ; Thomas-White K; Fok C; Kliethermes S; Schreckenberger PC; Brubaker L; Gai X; Wolfe AJ
[Ad] Endereço:Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois, USA.
[Ti] Título:The female urinary microbiome: a comparison of women with and without urgency urinary incontinence.
[So] Source:MBio;5(4):e01283-14, 2014 Jul 08.
[Is] ISSN:2150-7511
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial DNA and live bacteria have been detected in human urine in the absence of clinical infection, challenging the prevailing dogma that urine is normally sterile. Urgency urinary incontinence (UUI) is a poorly understood urinary condition characterized by symptoms that overlap urinary infection, including urinary urgency and increased frequency with urinary incontinence. The recent discovery of the urinary microbiome warrants investigation into whether bacteria contribute to UUI. In this study, we used 16S rRNA gene sequencing to classify bacterial DNA and expanded quantitative urine culture (EQUC) techniques to isolate live bacteria in urine collected by using a transurethral catheter from women with UUI and, in comparison, a cohort without UUI. For these cohorts, we demonstrated that the UUI and non-UUI urinary microbiomes differ by group based on both sequence and culture evidences. Compared to the non-UUI microbiome, sequencing experiments revealed that the UUI microbiome was composed of increased Gardnerella and decreased Lactobacillus. Nine genera (Actinobaculum, Actinomyces, Aerococcus, Arthrobacter, Corynebacterium, Gardnerella, Oligella, Staphylococcus, and Streptococcus) were more frequently cultured from the UUI cohort. Although Lactobacillus was isolated from both cohorts, distinctions existed at the species level, with Lactobacillus gasseri detected more frequently in the UUI cohort and Lactobacillus crispatus most frequently detected in controls. Combined, these data suggest that potentially important differences exist in the urinary microbiomes of women with and without UUI, which have strong implications in prevention, diagnosis, or treatment of UUI. Importance: New evidence indicates that the human urinary tract contains microbial communities; however, the role of these communities in urinary health remains to be elucidated. Urgency urinary incontinence (UUI) is a highly prevalent yet poorly understood urinary condition characterized by urgency, frequency, and urinary incontinence. Given the significant overlap of UUI symptoms with those of urinary tract infections, it is possible that UUI may have a microbial component. We compared the urinary microbiomes of women affected by UUI to those of a comparison group without UUI, using both high-throughput sequencing and extended culture techniques. We identified statistically significant differences in the frequency and abundance of bacteria present. These differences suggest a potential role for the urinary microbiome in female urinary health.
[Mh] Termos MeSH primário: Microbiota/fisiologia
RNA Ribossômico 16S/genética
Incontinência Urinária/microbiologia
Sistema Urinário/microbiologia
[Mh] Termos MeSH secundário: Actinomyces/genética
Actinomyces/isolamento & purificação
Aerococcus/genética
Aerococcus/isolamento & purificação
Idoso
Arthrobacter/genética
Arthrobacter/isolamento & purificação
Corynebacterium/genética
Corynebacterium/isolamento & purificação
Feminino
Gardnerella/genética
Gardnerella/isolamento & purificação
Seres Humanos
Lactobacillus/genética
Lactobacillus/isolamento & purificação
Microbiota/genética
Meia-Idade
Staphylococcus/genética
Staphylococcus/isolamento & purificação
Streptococcus/genética
Streptococcus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:161215
[Lr] Data última revisão:
161215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140710
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE


  8 / 29 MEDLINE  
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[PMID]:24327088
[Au] Autor:Swidsinski A; Loening-Baucke V; Mendling W; Dörffel Y; Schilling J; Halwani Z; Jiang XF; Verstraelen H; Swidsinski S
[Ad] Endereço:Charité Hospital, CCM, Laboratory for Molecular Genetics, Polymicrobial Infections and Bacterial Biofilms and Department of Medicine, Gastroenterology, Universitätsmedizin Berlin, Germany. alexander.swidsinski@charite.de.
[Ti] Título:Infection through structured polymicrobial Gardnerella biofilms (StPM-GB).
[So] Source:Histol Histopathol;29(5):567-87, 2014 May.
[Is] ISSN:1699-5848
[Cp] País de publicação:Spain
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: We analysed data on bacterial vaginosis (BV) contradicting the paradigm of mono-infection. METHODOLOGY: Tissues and epithelial cells of vagina, uterus, fallopian tubes and perianal region were investigated using fluorescence in situ hybridization (FISH) in women with BV and controls. RESULTS: Healthy vagina was free of biofilms. Prolific structured polymicrobial (StPM) Gardnerella-dominated biofilm characterised BV. The intact StPM-Gardnerella-biofilm enveloped desquamated vaginal/prepuce epithelial cells and was secreted with urine and sperma. The disease involved both genders and occurred in pairs. Children born to women with BV were negative. Monotherapy with metronidazole, moxifloxacin or local antiseptics suppressed but often did not eradicate StPM-Gardnerella-biofilms. There was no BV without Gardnerella, but Gardnerella was not BV. Outside of StPM-biofilm, Gardnerella was also found in a subset of children and healthy adults, but was dispersed, temporal and did not transform into StPM-Gardnerella-biofilm. CONCLUSIONS: StPM-Gardnerella-biofilm is an infectious subject. The assembly of single players to StPM-Gardnerella-biofilm is a not trivial every day process, but probably an evolutionary event with a long history of growth, propagation and selection for viability and ability to reshape the environment. The evolutionary memory is cemented in the structural differentiation of StPM-Gardnerella-biofilms and imparts them to resist previous and emerging challenges.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Gardnerella/patogenicidade
Infecções por Bactérias Gram-Positivas/microbiologia
Vaginose Bacteriana/microbiologia
[Mh] Termos MeSH secundário: Biofilmes/efeitos dos fármacos
Candidíase Vulvovaginal/microbiologia
Estudos de Casos e Controles
Feminino
Gardnerella/efeitos dos fármacos
Gardnerella/genética
Infecções por Bactérias Gram-Positivas/tratamento farmacológico
Seres Humanos
Hibridização in Situ Fluorescente
Masculino
Gravidez
Vagina/microbiologia
Vaginose Bacteriana/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1505
[Cu] Atualização por classe:161202
[Lr] Data última revisão:
161202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131212
[St] Status:MEDLINE
[do] DOI:10.14670/HH-29.10.567


  9 / 29 MEDLINE  
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[PMID]:23075892
[Au] Autor:Wei Q; Fu B; Liu J; Zhang Z; Zhao T
[Ad] Endereço:Department of Pathology at The Third Affiliated Hospital, Southern Medical University, Guangzhou, China.
[Ti] Título:Candida albicans and bacterial vaginosis can coexist on Pap smears.
[So] Source:Acta Cytol;56(5):515-9, 2012.
[Is] ISSN:0001-5547
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The aim of our study was to study the relationship of blastospores and pseudohyphae in Papanicolaou (Pap) smears and Nugent scores for bacterial vaginosis (BV). STUDY DESIGN: A total of 471 Pap smears with Candida albicans were reviewed. The presence of blastospores and pseudohyphae was established. The Pap smears were restained with the Gram stain method to evaluate the bacterial flora according to the Nugent scoring system. RESULTS: Of the 471 Pap smears, blastospores and pseudohyphae were observed in 62.8% (296/471) and 37.2% (175/471) of the smears, and displayed symptoms in 4.4% (13/296) and 43.4% (76/175), respectively. A significant difference was found between these 2 groups (p < 0.0001). A positive BV Nugent score (≥ 7) was found in 22.1% (104/471) of the C. albicans cases. Blastospores and pseudohyphae with BV were 14.2% (42/296) and 35.4% (62/175), respectively. These high Nugent scores indicate statistically significant differences (p < 0.0001). CONCLUSION: C. albicans and BV can coexist. The presence of blastospores in these C. albicans cases was negatively related to symptoms.
[Mh] Termos MeSH primário: Candida albicans/isolamento & purificação
Candidíase Vulvovaginal/diagnóstico
Teste de Papanicolaou
Esfregaço Vaginal
Vaginose Bacteriana/diagnóstico
[Mh] Termos MeSH secundário: Adulto
Idoso
Candidíase Vulvovaginal/complicações
Candidíase Vulvovaginal/microbiologia
Feminino
Gardnerella/isolamento & purificação
Seres Humanos
Hifas/isolamento & purificação
Lactobacillus/isolamento & purificação
Meia-Idade
Mobiluncus/isolamento & purificação
Vaginose Bacteriana/complicações
Vaginose Bacteriana/microbiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1301
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121019
[St] Status:MEDLINE
[do] DOI:10.1159/000339155


  10 / 29 MEDLINE  
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[PMID]:23071487
[Au] Autor:Srinivasan U; Ponnaluri S; Villareal L; Gillespie B; Wen A; Miles A; Bucholz B; Marrs CF; Iyer RK; Misra D; Foxman B
[Ad] Endereço:Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, Michigan, United States of America. usha@umich.edu
[Ti] Título:Gram stains: a resource for retrospective analysis of bacterial pathogens in clinical studies.
[So] Source:PLoS One;7(10):e42898, 2012.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We demonstrate the feasibility of using qPCR on DNA extracted from vaginal Gram stain slides to estimate the presence and relative abundance of specific bacterial pathogens. We first tested Gram stained slides spiked with a mix of 10(8) cfu/ml of Escherichia coli and 10(5) cfu/ml of Lactobacillus acidophilus. Primers were designed for amplification of total and species-specific bacterial DNA based on 16S ribosomal gene regions. Sample DNA was pre-amplified with nearly full length 16S rDNA ribosomal gene fragment, followed by quantitative PCR with genera and species-specific 16S rDNA primers. Pre-amplification PCR increased the bacterial amounts; relative proportions of Escherichia coli and Lactobacillus recovered from spiked slides remained unchanged. We applied this method to forty two archived Gram stained slides available from a clinical trial of cerclage in pregnant women at high risk of preterm birth. We found a high correlation between Nugent scores based on bacterial morphology of Lactobacillus, Gardenerella and Mobiluncus and amounts of quantitative PCR estimated genus specific DNA (rrn copies) from Gram stained slides. Testing of a convenience sample of eight paired vaginal swabs and Gram stains freshly collected from healthy women found similar qPCR generated estimates of Lactobacillus proportions from Gram stained slides and vaginal swabs. Archived Gram stained slides collected from large scale epidemiologic and clinical studies represent a valuable, untapped resource for research on the composition of bacterial communities that colonize human mucosal surfaces.
[Mh] Termos MeSH primário: Técnicas de Tipagem Bacteriana/métodos
Violeta de Genciana
Fenazinas
Reação em Cadeia da Polimerase/métodos
Coloração e Rotulagem
[Mh] Termos MeSH secundário: DNA Ribossômico/genética
Escherichia coli/genética
Escherichia coli/isolamento & purificação
Feminino
Gardnerella/genética
Gardnerella/isolamento & purificação
Seres Humanos
Lactobacillus/genética
Lactobacillus/isolamento & purificação
Mobiluncus/genética
Mobiluncus/isolamento & purificação
Gravidez
RNA Ribossômico 16S/genética
Estudos Retrospectivos
Vagina/microbiologia
Vaginose Bacteriana/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA, Ribosomal); 0 (Gram's stain); 0 (Phenazines); 0 (RNA, Ribosomal, 16S); J4Z741D6O5 (Gentian Violet)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121017
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0042898



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