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  1 / 1011 MEDLINE  
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[PMID]:29346438
[Au] Autor:Sierra LJ; Brown AG; Barilá GO; Anton L; Barnum CE; Shetye SS; Soslowsky LJ; Elovitz MA
[Ad] Endereço:Maternal Child Health Research Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America.
[Ti] Título:Colonization of the cervicovaginal space with Gardnerella vaginalis leads to local inflammation and cervical remodeling in pregnant mice.
[So] Source:PLoS One;13(1):e0191524, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The role of the cervicovaginal (CV) microbiome in regulating cervical function during pregnancy is poorly understood. Gardnerella vaginalis (G. vaginalis) is the most common bacteria associated with the diagnosis of bacterial vaginosis (BV). While BV has been associated with preterm birth (PTB), clinical trials targeting BV do not decrease PTB rates. It remains unknown if G. vaginalis is capable of triggering molecular, biomechanical and cellular events that could lead to PTB. The objective of this study was to determine if cervicovaginal colonization with G. vaginalis, in pregnant mice, induced cervical remodeling and modified cervical function. CD-1 timed-pregnant mice received a 5X108 CFU/mL intravaginal inoculation of G. vaginalis or control on embryonic day 12 (E12) and E13. On E15, the mice were sacrificed and cervicovaginal fluid (CVF), amniotic fluid (AF), cervix, uterus, placentas and fetal membranes (FM) were collected. Genomic DNA was isolated from the CVF, placenta, uterus and FM and QPCR was performed to confirm colonization. IL-6 was measured in the CVF and AF and soluble e-cadherin (seCAD) was assessed in the CVF by ELISA. RNA was extracted from the cervices to evaluate IL-10, IL-8, IL-1ß, TNF-α, Tff-1, SPINK-5, HAS-1 and LOX expression via QPCR. Mucicarmine and trichrome staining was used to assess cervical mucin and collagen. Biomechanical properties of the cervix were studied using quasi-static tensile load-to-failure biomechanical tests. G. vaginalis successfully colonized the CV space. This colonization induced immune responses (increased IL-6 levels in CVF and AF, increased mRNA expression of cervical cytokines), altered the epithelial barrier (increased seCAD in the CVF), induced cervical remodeling (increased mucin production, altered collagen) and altered cervical biomechanical properties (a decrease in biomechanical modulus and an increase in maximum strain). The ability of G. vaginalis to induce these molecular, immune, cellular and biomechanical changes suggests that this bacterium may play a pathogenic role in premature cervical remodeling leading to PTB.
[Mh] Termos MeSH primário: Colo do Útero/microbiologia
Gardnerella vaginalis/isolamento & purificação
Inflamação/microbiologia
Vagina/microbiologia
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
Colo do Útero/patologia
Citocinas/metabolismo
Ensaio de Imunoadsorção Enzimática
Feminino
Gardnerella vaginalis/genética
Gardnerella vaginalis/crescimento & desenvolvimento
Camundongos
Gravidez
Reação em Cadeia da Polimerase em Tempo Real
Vaginose Bacteriana/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191524


  2 / 1011 MEDLINE  
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[PMID]:28972465
[Au] Autor:Carrillo-Ávila JA; Serrano-García ML; Fernández-Parra J; Sorlózano-Puerto A; Navarro-Marí JM; Stensvold CR; Gutiérrez-Fernández J
[Ad] Endereço:1​Biobank of the Andalusian Public Health System (BBSSPA), Granada, Spain.
[Ti] Título:Prevalence and genetic diversity of Trichomonas vaginalis in the general population of Granada and co-infections with Gardnerella vaginalis and Candida species.
[So] Source:J Med Microbiol;66(10):1436-1442, 2017 Oct.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Purulent or exudative genitourinary infections are a frequent cause of consultation in primary and specialized healthcare. The objectives of this study were: to determine the prevalence of Trichomonas vaginalis and co-infections with Candida spp. and Gardnerella vaginalis in vaginal secretion; and to use multilocus sequence typing (MLST) to analyse the genetic diversity of T. vaginalis strains. METHODOLOGY: The samples were submitted for analysis (n=5230) to a third-level hospital in Granada (Southern Spain) between 2011 and 2014; eight T. vaginalis strains isolated during 2015 were randomly selected for MLST analysis. Culture and nucleic acid hybridization techniques were used to detect microorganisms in the samples. RESULTS: The prevalence of T. vaginalis was 2.4 % between 2011 and 2014, being higher during the first few months of both 2011 and 2012. Among samples positive for T. vaginalis, co-infection with G. vaginalis was detected in 29 samples and co-infection with Candida spp. in 6, while co-infection with all three pathogens was observed in 3 samples. The only statistically significant between-year difference in co-infection rates was observed for T. vaginalis with G. vaginalis due to an elevated rate in 2011. MLST analysis results demonstrated a high genetic variability among strains circulating in our setting. CONCLUSION: These findings emphasize the need for the routine application of diagnostic procedures to avoid the spread of this sexually transmitted infection.
[Mh] Termos MeSH primário: Candida/classificação
Candidíase/complicações
Gardnerella vaginalis/isolamento & purificação
Variação Genética
Tricomoníase/microbiologia
Trichomonas vaginalis/genética
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Candidíase/epidemiologia
Coinfecção/epidemiologia
Coinfecção/microbiologia
Coinfecção/parasitologia
Feminino
Infecções por Bactérias Gram-Positivas/complicações
Infecções por Bactérias Gram-Positivas/epidemiologia
Infecções por Bactérias Gram-Positivas/microbiologia
Seres Humanos
Meia-Idade
Espanha/epidemiologia
Tricomoníase/complicações
Tricomoníase/epidemiologia
Doenças Vaginais/epidemiologia
Doenças Vaginais/microbiologia
Doenças Vaginais/parasitologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000603


  3 / 1011 MEDLINE  
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[PMID]:28846702
[Au] Autor:Abdelmaksoud AA; Girerd PH; Garcia EM; Brooks JP; Leftwich LM; Sheth NU; Bradley SP; Serrano MG; Fettweis JM; Huang B; Strauss JF; Buck GA; Jefferson KK
[Ad] Endereço:Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, United States of America.
[Ti] Título:Association between statin use, the vaginal microbiome, and Gardnerella vaginalis vaginolysin-mediated cytotoxicity.
[So] Source:PLoS One;12(8):e0183765, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bacterial vaginosis (BV) is the leading dysbiosis of the vaginal microbiome. The pathways leading towards the development of BV are not well understood. Gardnerella vaginalis is frequently associated with BV. G. vaginalis produces the cholesterol-dependent cytolysin (CDC), vaginolysin, which can lyse a variety of human cells and is thought to play a role in pathogenesis. Because membrane cholesterol is required for vaginolysin to function, and because HMG-CoA reductase inhibitors (statins) affect not only serum levels of cholesterol but membrane levels as well, we hypothesized that statins might affect the vaginal microbiome. METHODS: To investigate the relationship between use of the statins and the vaginal microbiome, we analyzed 16S rRNA gene taxonomic surveys performed on vaginal samples from 133 women who participated in the Vaginal Human Microbiome Project and who were taking statins at the time of sampling, 152 women who reported high cholesterol levels but were not taking statins, and 316 women who did not report high cholesterol. To examine the effect of statins on the cytolytic effect of vaginolysin, the cholesterol-dependent cytolysin (CDC) produced by Gardnerella vaginalis, we assessed the effect of simvastatin pretreatment of VK2E6/E7 vaginal epithelial cells on vaginolysin-mediated cytotoxicity. RESULTS: The mean proportion of G. vaginalis among women taking statins was significantly lower relative to women not using statins. Women using statins had higher mean proportions of Lactobacillus crispatus relative to women with normal cholesterol levels, and higher levels of Lactobacillus jensenii relative to women with high cholesterol but not taking statins. In vitro, vaginal epithelial cells pretreated with simvastatin were relatively resistant to vaginolysin and this effect was inhibited by cholesterol. CONCLUSIONS: In this cross-sectional study, statin use was associated with reduced proportions of G. vaginalis and greater proportions of beneficial lactobacilli within the vaginal microbiome. The negative association between statin use and G. vaginalis may be related to inhibition of vaginolysin function.
[Mh] Termos MeSH primário: Proteínas de Bactérias/fisiologia
Sobrevivência Celular/fisiologia
Gardnerella vaginalis/metabolismo
Inibidores de Hidroximetilglutaril-CoA Redutases/farmacologia
Microbiota/efeitos dos fármacos
Sinvastatina/farmacologia
Vagina/microbiologia
[Mh] Termos MeSH secundário: Toxinas Bacterianas
Contagem de Colônia Microbiana
Células Epiteliais/metabolismo
Feminino
Gardnerella vaginalis/isolamento & purificação
Seres Humanos
Microbiota/genética
Meia-Idade
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacterial Toxins); 0 (Hydroxymethylglutaryl-CoA Reductase Inhibitors); 0 (RNA, Ribosomal, 16S); 0 (vaginolysin, Gardnerella vaginalis); AGG2FN16EV (Simvastatin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183765


  4 / 1011 MEDLINE  
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[PMID]:28792372
[Au] Autor:Ferreira CST; Donders GG; Parada CMGL; Tristão ADR; Fernandes T; da Silva MG; Marconi C
[Ad] Endereço:1​Department of Pathology, Botucatu Medical School, UNESP - Univ Estadual Paulista, Botucatu, Brazil.
[Ti] Título:Treatment failure of bacterial vaginosis is not associated with higher loads of Atopobium vaginae and Gardnerella vaginalis.
[So] Source:J Med Microbiol;66(8):1217-1224, 2017 Aug.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Cervicovaginal Atopobium vaginae and Gardnerella vaginalis are strongly associated with bacterial vaginosis (BV) and are the main components of vaginal biofilms. The low efficacy of BV treatment with metronidazole may be due to the presence of such biofilms. Thus, the aim of this study was to compare the pretreatment cervicovaginal loads of A. vaginae and G. vaginalis for women who restored normal flora and those who persisted with BV after a full course of oral metronidazole. METHODOLOGY: In this cross-sectional study, 309 reproductive-aged women were recruited in a primary health care service in Botucatu, Brazil. Cervicovaginal samples were tested for genital tract infections, microscopic classification of local microbiota and molecular quantification of A. vaginae and G. vaginalis. RESULTS: All the participants with concurrent cervicovaginal infections (n=64) were excluded. A total of 84 out of 245 (34.3 %) women had BV at enrolment and 43 (51.2 %) of them completed the treatment and returned for follow-up. Evaluation of the vaginal microbiota at follow-up showed that 29 (67.4 %) women restored normal vaginal flora, while 14 (32.6 %) still had BV. The pretreatment loads of G. vaginalis were lower in women with treatment failure (P=0.001) compared to those who successfully restored normal flora. The loads of A. vaginae did not differ between the groups. CONCLUSION: Although G. vaginalis produces several virulence factors and its loads correlate positively with those of A. vaginae, higher cervicovaginal quantities of these bacteria are not associated with treatment failure of BV after oral metronidazole.
[Mh] Termos MeSH primário: Actinobacteria/efeitos dos fármacos
Gardnerella vaginalis/efeitos dos fármacos
Vaginose Bacteriana/tratamento farmacológico
[Mh] Termos MeSH secundário: Actinobacteria/genética
Actinobacteria/isolamento & purificação
Actinobacteria/fisiologia
Adolescente
Adulto
Carga Bacteriana/efeitos dos fármacos
Brasil
Feminino
Gardnerella vaginalis/genética
Gardnerella vaginalis/isolamento & purificação
Gardnerella vaginalis/fisiologia
Seres Humanos
Meia-Idade
Falha de Tratamento
Vagina/microbiologia
Vaginose Bacteriana/microbiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000561


  5 / 1011 MEDLINE  
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[PMID]:28594946
[Au] Autor:Hardy L; Jespers V; De Baetselier I; Buyze J; Mwambarangwe L; Musengamana V; van de Wijgert J; Crucitti T
[Ad] Endereço:HIV and Sexual Health Unit, Department of Public Health, Institute of Tropical Medicine, Antwerp, Belgium.
[Ti] Título:Association of vaginal dysbiosis and biofilm with contraceptive vaginal ring biomass in African women.
[So] Source:PLoS One;12(6):e0178324, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We investigated the presence, density and bacterial composition of contraceptive vaginal ring biomass and its association with the vaginal microbiome. Of 415 rings worn by 120 Rwandese women for three weeks, the biomass density was assessed with crystal violet and the bacterial composition of biomass eluates was assessed with quantitative polymerase chain reaction (qPCR). The biomass was visualised after fluorescence in situ hybridisation (FISH) and with scanning electron microscopy (SEM). The vaginal microbiome was assessed with Nugent scoring and vaginal biofilm was visualised after FISH. All vaginal rings were covered with biomass (mean optical density (OD) of 3.36; standard deviation (SD) 0.64). Lactobacilli were present on 93% of the rings, Gardnerella vaginalis on 57%, and Atopobium vaginae on 37%. The ring biomass density was associated with the concentration of A. vaginae (OD +0.03; 95% confidence interval (CI) 0.01-0.05 for one log increase; p = 0.002) and of G. vaginalis (OD +0.03; (95% CI 0.01-0.05; p = 0.013). The density also correlated with Nugent score: rings worn by women with a BV Nugent score (mean OD +0.26), and intermediate score (mean OD +0.09) had a denser biomass compared to rings worn by participants with a normal score (p = 0.002). Furthermore, presence of vaginal biofilm containing G. vaginalis (p = 0.001) and A. vaginae (p = 0.005) correlated with a denser ring biomass (mean OD +0.24 and +0.22 respectively). With SEM we observed either a loose network of elongated bacteria or a dense biofilm. We found a correlation between vaginal dysbiosis and the density and composition of the ring biomass, and further research is needed to determine if these relationships are causal. As multipurpose vaginal rings to prevent pregnancy, HIV, and other sexually transmitted diseases are being developed, the potential impact of ring biomass on the vaginal microbiota and the release of active pharmaceutical ingredients should be researched in depth.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Dispositivos Anticoncepcionais Femininos
Disbiose/microbiologia
Vagina/microbiologia
[Mh] Termos MeSH secundário: Adulto
África
Biomassa
Feminino
Gardnerella vaginalis/patogenicidade
Seres Humanos
Microbiota/fisiologia
Microscopia Eletrônica de Varredura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178324


  6 / 1011 MEDLINE  
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[PMID]:28583109
[Au] Autor:Janulaitiene M; Paliulyte V; Grinceviciene S; Zakareviciene J; Vladisauskiene A; Marcinkute A; Pleckaityte M
[Ad] Endereço:Institute of Biotechnology, Vilnius University, Sauletekio al. 7, 10257, Vilnius, Lithuania.
[Ti] Título:Prevalence and distribution of Gardnerella vaginalis subgroups in women with and without bacterial vaginosis.
[So] Source:BMC Infect Dis;17(1):394, 2017 Jun 05.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bacterial vaginosis (BV) is one of the leading causes of vaginal complaints among women of childbearing age. The role of Gardnerella vaginalis remains controversial due to its presence in healthy and BV-type vaginal microflora. The phenotypic and genotypic heterogeneity of G. vaginalis suggested the existence of strain variants linked with different health conditions. We sought to analyze prevalence and distribution of G. vaginalis subgroups (clades) in BV-positive (n = 29), partial BV (n = 27), and BV-negative (n = 53) vaginal samples from Lithuanian women. METHODS: Vaginal samples were characterized by Amsel criteria and the Nugent method. Bacterial signatures characteristic of BV and concomitant infections were identified by culture and PCR. Using singleplex PCR assays, G. vaginalis subgroups were identified in 109 noncultured vaginal specimens by targeting clade-specific genes. Isolated G. vaginalis clinical strains were subtyped and the presence of the sialidase coding gene was detected by PCR. Data analysis was performed using GraphPad Prism statistical software. RESULTS: G. vaginalis was found in 87% of women without BV. Clade 4 was most frequently detected (79.4%), followed by clade 1 (63.7%), clade 2 (42.2%), and clade 3 (15.7%). Multi-clade G. vaginalis communities showed a positive association with Nugent score (NS) ≥ 4 (OR 3.64; 95% CI 1.48-8.91; p = 0.005). Clade 1 and clade 2 were statistically significantly more common in samples with NS 7-10 (OR 4.69; 95% CI 1.38-15.88; p = 0.01 and OR 6.26; 95% CI 2.20-17.81; p ≤ 0.001, respectively). Clade 3 and clade 4 showed no association with high NS (OR 0.88; 95% CI 0.26-3.04; p = 1.00 and OR 1.31; 95% CI 0.39-4.41; p = 0.767, respectively). The gene coding for sialidase was detected in all isolates of clade 1 and clade 2, but not in clade 4 isolates. CONCLUSIONS: We showed an association between the microbial state of vaginal microflora and specific subgroups of G. vaginalis, the distribution of which may determine the clinical manifestation of BV. The frequent detection of clade 4 in the BV-negative samples might be due its lack of the gene coding for sialidase.
[Mh] Termos MeSH primário: Gardnerella vaginalis/genética
Gardnerella vaginalis/patogenicidade
Infecções por Bactérias Gram-Positivas/microbiologia
Vagina/microbiologia
Vaginose Bacteriana/microbiologia
[Mh] Termos MeSH secundário: Adulto
Feminino
Genótipo
Infecções por Bactérias Gram-Positivas/epidemiologia
Seres Humanos
Lituânia/epidemiologia
Meia-Idade
Neuraminidase/genética
Reação em Cadeia da Polimerase/métodos
Vaginose Bacteriana/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.2.1.18 (Neuraminidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2501-y


  7 / 1011 MEDLINE  
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[PMID]:28358889
[Au] Autor:Gilbert NM; O'Brien VP; Lewis AL
[Ad] Endereço:Department of Obstetrics and Gynecology, Center for Reproductive Health Sciences, Washington University School of Medicine, St. Louis, Missouri, United States of America.
[Ti] Título:Transient microbiota exposures activate dormant Escherichia coli infection in the bladder and drive severe outcomes of recurrent disease.
[So] Source:PLoS Pathog;13(3):e1006238, 2017 Mar.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pathogens often inhabit the body asymptomatically, emerging to cause disease in response to unknown triggers. In the bladder, latent intracellular Escherichia coli reservoirs are regarded as likely origins of recurrent urinary tract infection (rUTI), a problem affecting millions of women worldwide. However, clinically plausible triggers that activate these reservoirs are unknown. Clinical studies suggest that the composition of a woman's vaginal microbiota influences her susceptibility to rUTI, but the mechanisms behind these associations are unclear. Several lines of evidence suggest that the urinary tract is routinely exposed to vaginal bacteria, including Gardnerella vaginalis, a dominant member of the vaginal microbiota in some women. Using a mouse model, we show that bladder exposure to G. vaginalis triggers E. coli egress from latent bladder reservoirs and enhances the potential for life-threatening outcomes of the resulting E. coli rUTI. Transient G. vaginalis exposures were sufficient to cause bladder epithelial apoptosis and exfoliation and interleukin-1-receptor-mediated kidney injury, which persisted after G. vaginalis clearance from the urinary tract. These results support a broader view of UTI pathogenesis in which disease can be driven by short-lived but powerful urinary tract exposures to vaginal bacteria that are themselves not "uropathogenic" in the classic sense. This "covert pathogenesis" paradigm may apply to other latent infections, (e.g., tuberculosis), or for diseases currently defined as noninfectious because routine culture fails to detect microbes of recognized significance.
[Mh] Termos MeSH primário: Infecções por Escherichia coli/microbiologia
Bexiga Urinária/microbiologia
Infecções Urinárias/microbiologia
Vagina/microbiologia
[Mh] Termos MeSH secundário: Animais
Doença Crônica
Modelos Animais de Doenças
Feminino
Imunofluorescência
Gardnerella vaginalis
Imuno-Histoquímica
Camundongos
Camundongos Endogâmicos C57BL
Microscopia Eletrônica de Varredura
Escherichia coli Uropatogênica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170930
[Lr] Data última revisão:
170930
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006238


  8 / 1011 MEDLINE  
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[PMID]:28339292
[Au] Autor:Machado D; Gaspar C; Palmeira-de-Oliveira A; Cavaleiro C; Salgueiro L; Martinez-de-Oliveira J; Cerca N
[Ad] Endereço:Centre of Biological Engineering, LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
[Ti] Título:Thymbra capitata essential oil as potential therapeutic agent against Gardnerella vaginalis biofilm-related infections.
[So] Source:Future Microbiol;12:407-416, 2017 Apr.
[Is] ISSN:1746-0921
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: To evaluate the antibacterial activity of Thymbra capitata essential oil and its main compound, carvacrol, against Gardnerella vaginalis grown planktonically and as biofilms, and its effect of vaginal lactobacilli. MATERIALS & METHODS: Minimal inhibitory concentration, minimal lethal concentration determination and flow cytometry analysis were used to assess the antibacterial effect against planktonic cells. Antibiofilm activity was measured through quantification of biomass and visualization of biofilm structure by confocal laser scanning microscopy. RESULTS: T. capitata essential oil and carvacrol exhibited a potent antibacterial activity against G. vaginalis cells. Antibiofilm activity was more evident with the essential oil than carvacrol. Furthermore, vaginal lactobacilli were significantly more tolerant to the essential oil. CONCLUSION: T. capitata essential oil stands up as a promising therapeutic agent against G. vaginalis biofilm-related infections.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Biofilmes/efeitos dos fármacos
Gardnerella vaginalis/efeitos dos fármacos
Lamiaceae/química
Monoterpenos/farmacologia
Óleos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/isolamento & purificação
Citometria de Fluxo
Gardnerella vaginalis/fisiologia
Lactobacillus/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Viabilidade Microbiana/efeitos dos fármacos
Microscopia Confocal
Monoterpenos/isolamento & purificação
Óleos Voláteis/isolamento & purificação
Espectrofotometria
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Monoterpenes); 0 (Oils, Volatile); 9B1J4V995Q (carvacrol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.2217/fmb-2016-0184


  9 / 1011 MEDLINE  
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[PMID]:28241058
[Au] Autor:Hardy L; Jespers V; Van den Bulck M; Buyze J; Mwambarangwe L; Musengamana V; Vaneechoutte M; Crucitti T
[Ad] Endereço:HIV and Sexual Health Group, Department of Public Health, Institute of Tropical Medicine, Antwerp, Belgium.
[Ti] Título:The presence of the putative Gardnerella vaginalis sialidase A gene in vaginal specimens is associated with bacterial vaginosis biofilm.
[So] Source:PLoS One;12(2):e0172522, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial vaginosis (BV) is a difficult-to-treat recurrent condition in which health-associated lactobacilli are outnumbered by other anaerobic bacteria, such as Gardnerella vaginalis. Certain genotypes of G. vaginalis can produce sialidase, while others cannot. Sialidase is known to facilitate the destruction of the protective mucus layer on the vaginal epithelium by hydrolysis of sialic acid on the glycans of mucous membranes. This process possibly facilitates adhesion of bacterial cells on the epithelium since it has been linked with the development of biofilm in other pathogenic conditions. Although it has not been demonstrated yet, it is probable that G. vaginalis benefits from this mechanism by attaching to the vaginal epithelium to initiate biofilm development. In this study, using vaginal specimens of 120 women enrolled in the Ring Plus study, we assessed the association between the putative G. vaginalis sialidase A gene by quantitative polymerase chain reaction (qPCR), the diagnosis of BV according to Nugent score, and the occurrence of a BV-associated biofilm dominated by G. vaginalis by fluorescence in situ hybridisation (FISH). We detected the putative sialidase A gene in 75% of the G. vaginalis-positive vaginal specimens and found a strong association (p<0.001) between the presence of a G. vaginalis biofilm, the diagnosis of BV according to Nugent and the detection of high loads of the G. vaginalis sialidase A gene in the vaginal specimens. These results could redefine diagnosis of BV, and in addition might guide research for new treatment.
[Mh] Termos MeSH primário: Biofilmes
Gardnerella vaginalis/enzimologia
Neuraminidase/genética
Vagina/microbiologia
Vaginose Bacteriana/enzimologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Feminino
Gardnerella vaginalis/genética
Seres Humanos
Hidrólise
Hibridização in Situ Fluorescente
Microscopia Confocal
Membrana Mucosa/microbiologia
Ácido N-Acetilneuramínico/química
Ácidos Nucleicos Peptídicos/química
Reação em Cadeia da Polimerase
Polissacarídeos/química
Ruanda
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptide Nucleic Acids); 0 (Polysaccharides); EC 3.2.1.18 (Neuraminidase); GZP2782OP0 (N-Acetylneuraminic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172522


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[PMID]:28234976
[Au] Autor:Vodstrcil LA; Twin J; Garland SM; Fairley CK; Hocking JS; Law MG; Plummer EL; Fethers KA; Chow EP; Tabrizi SN; Bradshaw CS
[Ad] Endereço:Melbourne Sexual Health Centre, Alfred Health, Carlton, Melbourne, Australia.
[Ti] Título:The influence of sexual activity on the vaginal microbiota and Gardnerella vaginalis clade diversity in young women.
[So] Source:PLoS One;12(2):e0171856, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To examine the influence of sexual activity on the composition and consistency of the vaginal microbiota over time, and distribution of Gardnerella vaginalis clades in young women. METHODS: Fifty-two participants from a university cohort were selected. Vaginal swabs were self-collected every 3-months for up to 12 months with 184 specimens analysed. The vaginal microbiota was characterised using Roche 454 V3/4 region 16S rRNA sequencing, and G.vaginalis clade typing by qPCR. RESULTS: A Lactobacillus crispatus dominated vaginal microbiota was associated with Caucasian ethnicity (adjusted relative risk ratio[ARRR] = 7.28, 95%CI:1.37,38.57,p = 0.020). An L.iners (ARRR = 17.51, 95%CI:2.18,140.33,p = 0.007) or G.vaginalis (ARRR = 14.03, 95%CI:1.22,160.69, p = 0.034) dominated microbiota was associated with engaging in penile-vaginal sex. Microbiota dominated by L.crispatus, L.iners or other lactobacilli exhibited greater longitudinal consistency of the bacterial communities present compared to ones dominated by heterogeneous non-lactobacilli (p<0.030); sexual activity did not influence consistency. Women who developed BV were more likely to have clade GV4 compared to those reporting no sex/practiced non-coital activities (OR = 11.82, 95%CI:1.87,74.82,p = 0.009). Specimens were more likely to contain multiple G.vaginalis clades rather than a single clade if women engaged in penile-vaginal sex (RRR = 9.55, 95%CI:1.33,68.38,p = 0.025) or were diagnosed with BV (RRR = 31.5, 95%CI:1.69,586.87,p = 0.021). CONCLUSIONS: Sexual activity and ethnicity influenced the composition of the vaginal microbiota of these young, relatively sexually inexperienced women. Women had consistent vaginal microbiota over time if lactobacilli were the dominant spp. present. Penile-vaginal sex did not alter the consistency of microbial communities but increased G.vaginalis clade diversity in young women with and without BV, suggesting sexual transmission of commensal and potentially pathogenic clades.
[Mh] Termos MeSH primário: Gardnerella vaginalis/genética
Lactobacillus/genética
Microbiota/genética
RNA Ribossômico 16S/genética
Vagina/microbiologia
[Mh] Termos MeSH secundário: Adolescente
Grupo com Ancestrais do Continente Asiático
Austrália
Biodiversidade
Coito/fisiologia
Grupo com Ancestrais do Continente Europeu
Feminino
Gardnerella vaginalis/classificação
Gardnerella vaginalis/isolamento & purificação
Seres Humanos
Lactobacillus/classificação
Lactobacillus/isolamento & purificação
Estudos Longitudinais
Masculino
Filogenia
Vaginose Bacteriana/diagnóstico
Vaginose Bacteriana/etnologia
Vaginose Bacteriana/microbiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171856



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