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[PMID]:29269698
[Au] Autor:Nakanishi K; Kawano H; Amano T; Omori Y; Kanma H; Hirano T
[Ad] Endereço:Department of Stroke and Cerebrovascular Medicine, Kyorin University.
[Ti] Título:[Stroke due to infective endocarditis diagnosed by the retrieved thrombus: a case report].
[So] Source:Rinsho Shinkeigaku;58(1):35-40, 2018 Jan 26.
[Is] ISSN:1882-0654
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:A 80-years-old woman suddenly presented with aphasia, right hemiparesis, and dysesthesia. MRA showed the left middle cerebral artery occlusion. She was diagnosed as hyperacute ischemic stroke. She was treated with intravenous recombinant tissue plasminogen activator and underwent endovascular thrombectomy. On admission, she had a fever and high C reactive protein, and was treated with antibiotic therapy. The pathological diagnosis of the retrieved thrombus revealed the cluster of the gram positive cocci. The blood culture was negative and thransthoracic echocardiogram did not detect the vegetation. She was finally diagnosed as cardioembolic stroke due to infective endocarditis based on the pathological diagnosis of the retrieved thrombus. The pathological diagnosis of the retrieved thrombus was quite important to clarify the cause of ischemic stroke.
[Mh] Termos MeSH primário: Endocardite/complicações
Endocardite/diagnóstico
Procedimentos Endovasculares/métodos
Infecções por Bactérias Gram-Positivas
Acidente Vascular Cerebral/etiologia
Acidente Vascular Cerebral/terapia
Trombectomia/métodos
Trombose/complicações
Trombose/microbiologia
[Mh] Termos MeSH secundário: Idoso de 80 Anos ou mais
Endocardite/microbiologia
Endocardite/patologia
Feminino
Cocos Gram-Positivos
Seres Humanos
Infusões Intravenosas
Angiografia por Ressonância Magnética
Acidente Vascular Cerebral/diagnóstico por imagem
Trombose/patologia
Ativador de Plasminogênio Tecidual/administração & dosagem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.4.21.68 (Tissue Plasminogen Activator)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171223
[St] Status:MEDLINE
[do] DOI:10.5692/clinicalneurol.cn-001099


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[PMID]:28631909
[Au] Autor:Cherednichenko AG; Kulchavenya EV
[Ad] Endereço:Novosibirsk Tuberculosis Research Institute of Minzdrav of Russia.
[Ti] Título:[Characterization of microbial flora in patients suspected of having urogenital tuberculosis].
[So] Source:Urologiia;(2):66-70, 2017 Jun.
[Is] ISSN:1728-2985
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:INTRODUCTION: In the structure of tuberculosis morbidity, urogenital tuberculosis (UGT) ranks second or third only to pulmonary tuberculosis. Every fourth patient has the stage of cavern formation at first presentation. Seventy five percent of nephrotuberculosis cases co-occur with nonspecific pyelonephritis, which excludes "sterile pyuria". We aimed to determine the microbial spectrum of urine and gonadal appendages in patients with suspected UGT and to investigate the susceptibility of the isolated microorganisms to the main antibacterial drugs. MATERIAL AND METHODS: The study investigated the spectrum of pathogenic microflora isolated from patients admitted to the urogenital clinic of the TB Research Institute from January 1, 2012 to September 30, 2016 to rule out UGT. Microorganism species were identified by time-of-flight mass spectrometry using the microbiological analyzer Phoenix 100 (Becton Dickinson, USA). Antibiotic resistance was determined using the Phoenix 100 microbiological analyzer (Becton Dickinson, USA). RESULTS: A total of 3004 bacteriological tests for nonspecific microflora were performed during the analyzed period. In the structure of pathogens, E. coli comprised about half of the cases thus confirming its role as the leading uropathogen. Only piperacillin/tazobactam and nitrofurantoin provided relatively plausible antimicrobial resistance rates, for other antibacterial drugs the rates of susceptible strains progressively decreased and by 2016 dropped to 40% and lower. CONCLUSION: Our findings cannot be extrapolated to the entire population since patients presenting for differential diagnosis receive multiple courses of treatment with various antibacterial drugs, which led to the selection of resistant strains. The findings emphasize the need for a strict selection of antibiotics for urogenital infection empirical therapy. In the absence of response, the patient should be immediately examined for tuberculosis using molecular and genetic diagnostic techniques.
[Mh] Termos MeSH primário: Resistência Microbiana a Medicamentos
Fungos/classificação
Bactérias Gram-Negativas/classificação
Cocos Gram-Positivos/classificação
Tuberculose Urogenital/microbiologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Antibacterianos/uso terapêutico
Fungos/efeitos dos fármacos
Fungos/isolamento & purificação
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/isolamento & purificação
Cocos Gram-Positivos/efeitos dos fármacos
Cocos Gram-Positivos/isolamento & purificação
Seres Humanos
Espectrometria de Massas
Testes de Sensibilidade Microbiana
Federação Russa
Tuberculose Urogenital/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE


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[PMID]:28414658
[Au] Autor:Le H; Prejean S; Heck M
[Ad] Endereço:University of Alabama at Birmingham School of Medicine.
[Ti] Título:Septic Cerebral Venosinus Thrombosis Secondary to an Odontogenic Infection.
[So] Source:J La State Med Soc;169(2):33-36, 2017 Mar-Apr.
[Is] ISSN:0024-6921
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cerebral venosinus thrombosis (CVT); is an uncommon, potentially fatal disease that is more common in young adults and children. Thrombophilia, elevated estrogenic states, and infections are the most common risk factors in patients who develop CVT. CASE: A 69-year-old man with a right-sided odontogenic infection presented with fever, headache, opthalmoplegia, and periorbital swelling. Imaging revealed evidence of meningitis and thrombosis of bilateral ophthalmic veins, the cavernous sinus, right internal jugular vein, and sigmoid sinus. The patient was treated with empiric antibiotic therapy and unfractionated heparin. He recovered with only mild impairment in right eye abduction. DISCUSSION: Early diagnosis and prompt treatment of CVT is vital in reducing the associated morbidity and mortality. Unfractionated or low molecular weight heparin may be safely used in CVT patients. Thrombolytic therapy is an option in clinically severe cases. Treatment also includes addressing the underlying cause and management of early complications.
[Mh] Termos MeSH primário: Trombose do Corpo Cavernoso/diagnóstico por imagem
Cocos Gram-Positivos/isolamento & purificação
Meningite/diagnóstico por imagem
Doenças da Boca/complicações
[Mh] Termos MeSH secundário: Idoso
Antibacterianos/uso terapêutico
Trombose do Corpo Cavernoso/tratamento farmacológico
Diagnóstico Precoce
Heparina/uso terapêutico
Seres Humanos
Masculino
Meningite/tratamento farmacológico
Doenças da Boca/microbiologia
Tomografia Computadorizada por Raios X/métodos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 9005-49-6 (Heparin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE


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[PMID]:28358885
[Au] Autor:Harlow BE; Lawrence LM; Harris PA; Aiken GE; Flythe MD
[Ad] Endereço:Department of Animal and Food Sciences, University of Kentucky, Lexington KY, United States of America.
[Ti] Título:Exogenous lactobacilli mitigate microbial changes associated with grain fermentation (corn, oats, and wheat) by equine fecal microflora ex vivo.
[So] Source:PLoS One;12(3):e0174059, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cereal grains are often included in equine diets. When starch intake exceeds foregut digestion starch will reach the hindgut, impacting microbial ecology. Probiotics (e.g., lactobacilli) are reported to mitigate GI dysbioses in other species. This study was conducted to determine the effect of exogenous lactobacilli on pH and the growth of amylolytic and lactate-utilizing bacteria. Feces were collected from 3 mature geldings fed grass hay with access to pasture. Fecal microbes were harvested by differential centrifugation, washed, and re-suspended in anaerobic media containing ground corn, wheat, or oats at 1.6% (w/v) starch and one of five treatments: Control (substrate only), L. acidophilus, L. buchneri, L. reuteri, or an equal mixture of all three (107 cells/mL, final concentration). After 24 h of incubation (37°C, 160 rpm), samples were collected for pH and enumerations of total amylolytics, Group D Gram-positive cocci (GPC; Enterococci, Streptococci), lactobacilli, and lactate-utilizing bacteria. Enumeration data were log transformed prior to ANOVA (SAS, v. 9.3). Lactobacilli inhibited pH decline in corn and wheat fermentations (P < 0.0001). Specifically, addition of either L. reuteri or L. acidophilus was most effective at mitigating pH decline with both corn and wheat fermentation, in which the greatest acidification occurred (P < 0.05). Exogenous lactobacilli decreased amylolytics, while increasing lactate-utilizers in corn and wheat fermentations (P < 0.0001). In oat fermentations, L. acidophilus and L. reuteri inhibited pH decline and increased lactate-utilizers while decreasing amylolytics (P < 0.0001). For all substrates, L. reuteri additions (regardless of viability) had the lowest number of GPC and the highest number of lactobacilli and lactate-utilizers (P < 0.05). There were no additive effects when lactobacilli were mixed. Exogenous lactobacilli decreased the initial (first 8 h) rate of starch catalysis when wheat was the substrate, but did not decrease total (24 h) starch utilization in any case. These results indicate that exogenous lactobacilli can impact the microbial community and pH of cereal grain fermentations by equine fecal microflora ex vivo. Additionally, dead (autoclaved) exogenous lactobacilli had similar effects as live lactobacilli on fermentation. This latter result indicates that the mechanism by which lactobacilli impact other amylolytic bacteria is not simple resource competition.
[Mh] Termos MeSH primário: Fezes/microbiologia
Cocos Gram-Positivos/metabolismo
Lactobacillus/metabolismo
Probióticos/metabolismo
[Mh] Termos MeSH secundário: Fenômenos Fisiológicos da Nutrição Animal
Animais
Avena/química
Digestão/fisiologia
Fezes/química
Fermentação/efeitos dos fármacos
Cocos Gram-Positivos/química
Cavalos/microbiologia
Concentração de Íons de Hidrogênio
Lactobacillus/química
Probióticos/química
Amido/química
Amido/metabolismo
Triticum/química
Zea mays/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9005-25-8 (Starch)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174059


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[PMID]:28128639
[Au] Autor:Savage E; Chothe S; Lintner V; Pierre T; Matthews T; Kariyawasam S; Miller D; Tewari D; Jayarao B
[Ad] Endereço:1 Penn State Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University , University Park, Pennsylvania.
[Ti] Título:Evaluation of Three Bacterial Identification Systems for Species Identification of Bacteria Isolated from Bovine Mastitis and Bulk Tank Milk Samples.
[So] Source:Foodborne Pathog Dis;14(3):177-187, 2017 Mar.
[Is] ISSN:1556-7125
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A study was conducted to evaluate Sensititre Automated Reading and Incubation System 2x System (ARIS), API (API), and Bruker MALDI-TOF MS (MALDI) bacterial species identification systems using 132 diverse bacterial isolates from bovine milk samples and bulk tank milk received at the Penn State Animal Diagnostic Laboratory. The results were compared with 16S rRNA gene sequence analysis, which served as the reference method for species identification. The ARIS, API, and MALDI identified 0%, 40%, and 33.4% of species classified as Gram-positive rod isolates belonging to genera Arthrobacter, Bacillus, Brachybacterium, Brevibacterium, and Corynebacterium, respectively. It was observed that 76.5%, 93.9%, and 96.9% of catalase-negative, Gram-positive cocci (n = 33; Aerococcus, Enterococcus, Lactococcus, Streptococcus) were correctly identified to the species level by ARIS, API, and MALDI, respectively, while 33.4%, 84.5%, and 97.7% of catalase-positive, Gram-positive cocci (n = 45; Kocuria, Staphylococcus) were correctly identified to their species by ARIS, API, and MALDI, respectively. A total of 48 isolates (Acinetobacter, Citrobacter, Enterobacter, Escherichia, Klebsiella, Pantoea, Pasteurella, Providencia, Pseduomonas, Serratia) of Gram-negative bacteria were examined, of which 85.4%, 93.7%, and 95.8% of the isolates were correctly identified to the species level by ARIS, API, and MALDI, respectively. In our laboratory, the MALDI had the least costs associated with consumables and reagents compared to ARIS, API, and 16S rRNA identification methods. Identification of bacterial species was accomplished in <2 h using MALDI and 24 h for ARIS, API, and 16S rRNA identification systems.
[Mh] Termos MeSH primário: Bactérias Gram-Negativas/isolamento & purificação
Cocos Gram-Positivos/isolamento & purificação
Bacilos Gram-Positivos/isolamento & purificação
Mastite Bovina/diagnóstico
Leite/microbiologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Feminino
Contaminação de Alimentos/análise
Microbiologia de Alimentos
Bactérias Gram-Negativas/classificação
Cocos Gram-Positivos/classificação
Bacilos Gram-Positivos/classificação
Mastite Bovina/microbiologia
RNA Ribossômico 16S/isolamento & purificação
Análise de Sequência de RNA
Especificidade da Espécie
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1089/fpd.2016.2222


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[PMID]:27959343
[Au] Autor:Martínez-Rubio R; Quiles-Puchalt N; Martí M; Humphrey S; Ram G; Smyth D; Chen J; Novick RP; Penadés JR
[Ad] Endereço:Departamento de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Moncada, Spain.
[Ti] Título:Phage-inducible islands in the Gram-positive cocci.
[So] Source:ISME J;11(4):1029-1042, 2017 Apr.
[Is] ISSN:1751-7370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The SaPIs are a cohesive subfamily of extremely common phage-inducible chromosomal islands (PICIs) that reside quiescently at specific att sites in the staphylococcal chromosome and are induced by helper phages to excise and replicate. They are usually packaged in small capsids composed of phage virion proteins, giving rise to very high transfer frequencies, which they enhance by interfering with helper phage reproduction. As the SaPIs represent a highly successful biological strategy, with many natural Staphylococcus aureus strains containing two or more, we assumed that similar elements would be widespread in the Gram-positive cocci. On the basis of resemblance to the paradigmatic SaPI genome, we have readily identified large cohesive families of similar elements in the lactococci and pneumococci/streptococci plus a few such elements in Enterococcus faecalis. Based on extensive ortholog analyses, we found that the PICI elements in the four different genera all represent distinct but parallel lineages, suggesting that they represent convergent evolution towards a highly successful lifestyle. We have characterized in depth the enterococcal element, EfCIV583, and have shown that it very closely resembles the SaPIs in functionality as well as in genome organization, setting the stage for expansion of the study of elements of this type. In summary, our findings greatly broaden the PICI family to include elements from at least three genera of cocci.
[Mh] Termos MeSH primário: Bacteriófagos/fisiologia
Regulação Bacteriana da Expressão Gênica/fisiologia
Ilhas Genômicas
Cocos Gram-Positivos/virologia
[Mh] Termos MeSH secundário: Sítios de Ligação Microbiológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE
[do] DOI:10.1038/ismej.2016.163


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[PMID]:27756620
[Au] Autor:Boyanova L; Markovska R; Mitov I
[Ad] Endereço:Department of Medical Microbiology, Medical University of Sofia, Zdrave Street 2, 1431 Sofia, Bulgaria. Electronic address: l.boyanova@hotmail.com.
[Ti] Título:Virulence arsenal of the most pathogenic species among the Gram-positive anaerobic cocci, Finegoldia magna.
[So] Source:Anaerobe;42:145-151, 2016 Dec.
[Is] ISSN:1095-8274
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This review focuses on the virulence arsenal of the most pathogenic species among Gram positive anaerobic cocci, Finegoldia magna according to recently published data from 2012 to 2016. Virulence factors like sortase dependent pili and F. magna adhesion factor (FAF) facilitate the start of the infection. Albumin binding protein (PAB) enhances F. magna survival. FAF, subtilisin-like extracellular serine protease (SufA) and superantigen protein L protect the bacteria from factors of innate defense system. SufA, capsule and tissue-destroying enzymes provide a deep penetration or spread of the infections and the protein L is associated with infection severity. Biofilm production results in infection chronification and complicated treatment as well as to persistence of multi-species biofilms. Resistance rates to quinolones (13.0->70%) and clindamycin (0-40.0%) are important, and resistance to penicillins (<4%), chloramphenicol (7.0%) and metronidazole (<7%) has been reported. F. magna should not be overlooked when present in monoinfections or mixed infections in humans.
[Mh] Termos MeSH primário: Adesinas Bacterianas/genética
Firmicutes/patogenicidade
Infecções por Bactérias Gram-Positivas/patologia
Cocos Gram-Positivos/patogenicidade
Superantígenos/genética
Fatores de Virulência/genética
[Mh] Termos MeSH secundário: Adesinas Bacterianas/metabolismo
Anaerobiose
Antibacterianos/farmacologia
Biofilmes/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Farmacorresistência Bacteriana Múltipla/genética
Fímbrias Bacterianas/genética
Fímbrias Bacterianas/metabolismo
Firmicutes/efeitos dos fármacos
Firmicutes/genética
Firmicutes/isolamento & purificação
Expressão Gênica
Infecções por Bactérias Gram-Positivas/tratamento farmacológico
Infecções por Bactérias Gram-Positivas/microbiologia
Cocos Gram-Positivos/efeitos dos fármacos
Cocos Gram-Positivos/genética
Cocos Gram-Positivos/isolamento & purificação
Seres Humanos
Subtilisinas/genética
Subtilisinas/metabolismo
Superantígenos/metabolismo
Virulência
Fatores de Virulência/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Adhesins, Bacterial); 0 (Anti-Bacterial Agents); 0 (Superantigens); 0 (Virulence Factors); EC 3.4.21.- (Subtilisins)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


  8 / 1319 MEDLINE  
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[PMID]:27592459
[Au] Autor:Arai T; Ohta S; Tsurukiri J; Kumasaka K; Nagata K; Okita T; Oomura T; Hoshiai A; Koyama M; Yukioka T
[Ad] Endereço:Department of Emergency and Critical Care Medicine, Trauma and Emergency Center, Hachioji Medical Center of Tokyo Medical University, Tokyo 193-0998, Japan. Electronic address: qqaraitakao@yahoo.co.jp.
[Ti] Título:Procalcitonin levels predict to identify bacterial strains in blood cultures of septic patients.
[So] Source:Am J Emerg Med;34(11):2150-2153, 2016 Nov.
[Is] ISSN:1532-8171
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: We examined whether the values obtained from principal component analysis (PCA) on laboratory tests can be used to predict bacterial infections and identify bacterial strains in blood culture (BC). METHOD: This study is a single-center retrospective analysis of 315 patients suspected of having sepsis. We applied PCA on procalcitonin (PCT) and laboratory test biomarkers, namely, platelet (PLT), white blood cell, and C-reactive protein (CRP) as well as BC. RESULTS: Principal component analysis showed that PCT, CRP, and PLT contributions to component 1 were associated with bacterial infection. The number of patients who had BC-negative results, gram-positive cocci (GPC), and gram-negative rods (GNRs) were 124, 28, and 19, respectively. The mean value of component 1 in GNR-positive patients was 1.58±1.41 and was significantly higher than that in GPC-positive patients (0.28±0.87; P<.0001). Furthermore, the mean values of component 1 in both GNR- and GPC-positive patients were significantly higher than that in BC-negative patients (-0.31±0.65; P<.0001 and P<.002, respectively). One certain range showing higher value more than 2.00 for component 1 and -1.00 for component 2 only included GNR-positive patients. There were no BC-positive patients who showed less than -1.00 for component 1. CONCLUSION: The present results obtained by PCA on laboratory tests involving PCT, PLT, white blood cell, and CRP suggest the potential of PCA-obtained values to not only predict bloodstream infection but also identify bacterial strains. This provides some clinical significance in the management of sepsis in acute care.
[Mh] Termos MeSH primário: Calcitonina/sangue
Infecções por Bactérias Gram-Negativas/sangue
Infecções por Bactérias Gram-Positivas/sangue
Sepse/sangue
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Hemocultura
Proteína C-Reativa/metabolismo
Feminino
Bactérias Gram-Negativas
Infecções por Bactérias Gram-Negativas/diagnóstico
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Positivas/diagnóstico
Infecções por Bactérias Gram-Positivas/microbiologia
Cocos Gram-Positivos
Seres Humanos
Contagem de Leucócitos
Masculino
Meia-Idade
Contagem de Plaquetas
Valor Preditivo dos Testes
Análise de Componente Principal
Estudos Retrospectivos
Sepse/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-12-9 (Calcitonin); 9007-41-4 (C-Reactive Protein)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160905
[St] Status:MEDLINE


  9 / 1319 MEDLINE  
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[PMID]:27520338
[Au] Autor:Barnini S; Brucculeri V; Morici P; Ghelardi E; Florio W; Lupetti A
[Ad] Endereço:Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy.
[Ti] Título:A new rapid method for direct antimicrobial susceptibility testing of bacteria from positive blood cultures.
[So] Source:BMC Microbiol;16(1):185, 2016 Aug 12.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Rapid identification and antimicrobial susceptibility testing (AST) of the causative agent(s) of bloodstream infections can lead to prompt appropriate antimicrobial therapy. To shorten species identification, in this study bacteria were recovered from monomicrobial blood cultures by serum separator tubes and spotted onto the target plate for direct MALDI-TOF MS identification. Proper antibiotics were selected for direct AST based on species identification. In order to obtain rapid AST results, bacteria were recovered from positive blood cultures by two different protocols: by serum separator tubes (further referred to as PR1), or after a short-term subculture in liquid medium (further referred to as PR2). The results were compared with those obtained by the method currently used in our laboratory consisting in identification by MALDI-TOF and AST by Vitek 2 or Sensititre on isolated colonies. RESULTS: The direct MALDI-TOF method concordantly identified with the current method 97.5 % of the Gram-negative bacteria and 96.1 % of the Gram-positive cocci contained in monomicrobial blood cultures. The direct AST by PR1 and PR2 for all isolate/antimicrobial agent combinations was concordant/correct with the current method for 87.8 and 90.5 % of Gram-negative bacteria and for 93.1 and 93.8 % of Gram-positive cocci, respectively. In particular, 100 % categorical agreement was found with levofloxacin for Enterobacteriaceae by both PR1 and PR2, and 99.0 and 100 % categorical agreement was observed with linezolid for Gram-positive cocci by PR1 and PR2, respectively. There was no significant difference in accuracy between PR1 and PR2 for Gram-negative bacteria and Gram-positive cocci. CONCLUSIONS: This newly described method seems promising for providing accurate AST results. Most importantly, these results would be available in a few hours from blood culture positivity, which would help clinicians to promptly confirm or streamline an effective antibiotic therapy in patients with bloodstream infections.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Bactérias/efeitos dos fármacos
Bactérias/isolamento & purificação
Técnicas de Tipagem Bacteriana/métodos
Hemocultura/métodos
Testes de Sensibilidade Microbiana/métodos
[Mh] Termos MeSH secundário: Bacteriemia/sangue
Bacteriemia/microbiologia
Técnicas de Tipagem Bacteriana/instrumentação
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/isolamento & purificação
Cocos Gram-Positivos/efeitos dos fármacos
Cocos Gram-Positivos/isolamento & purificação
Seres Humanos
Linezolida/farmacologia
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/instrumentação
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); ISQ9I6J12J (Linezolid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160814
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-016-0805-5


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[PMID]:27458228
[Au] Autor:El Samad Y; Lanoix JP; Bennis Y; Diouf M; Saroufim C; Brunschweiler B; Rousseau F; Joseph C; Hamdad F; Ait Amer Meziane M; Routier S; Schmit JL
[Ad] Endereço:Department of Infectious Diseases, Amiens University Hospital, Amiens, France youssefsamad@yahoo.fr.
[Ti] Título:Tolerability and Plasma Drug Level Monitoring of Prolonged Subcutaneous Teicoplanin Treatment for Bone and Joint Infections.
[So] Source:Antimicrob Agents Chemother;60(10):6365-8, 2016 Oct.
[Is] ISSN:1098-6596
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Teicoplanin is a key drug for the treatment of multiresistant staphylococcal bone and joint infections (BJI), yet can only be administered via a parenteral route. The objective of this study was to evaluate the safety and tolerability of subcutaneous (s.c.) teicoplanin for that indication over 42 days. Thirty patients with Gram-positive cocci BJI were included. Once the target of 25 to 40 mg/liter trough serum concentration was achieved, treatment was switched from an intravenous to an s.c. route. No discontinuation of teicoplanin related to injection site reaction and no severe local adverse event were observed. On multivariate analysis, better tolerability was observed at the beginning of treatment, in patients over 70 years old, and for dosages less than 600 mg. In conclusion, we recommend s.c. administration of teicoplanin when needed.
[Mh] Termos MeSH primário: Antibacterianos/administração & dosagem
Antibacterianos/sangue
Infecções por Bactérias Gram-Positivas/tratamento farmacológico
Teicoplanina/administração & dosagem
Teicoplanina/sangue
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Antibacterianos/uso terapêutico
Doenças Ósseas Infecciosas/tratamento farmacológico
Doenças Ósseas Infecciosas/microbiologia
Monitoramento de Medicamentos
Feminino
Cocos Gram-Positivos/patogenicidade
Seres Humanos
Injeções Subcutâneas
Artropatias/tratamento farmacológico
Artropatias/microbiologia
Masculino
Meia-Idade
Estudos Prospectivos
Teicoplanina/uso terapêutico
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 61036-62-2 (Teicoplanin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160727
[St] Status:MEDLINE
[do] DOI:10.1128/AAC.00351-16



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