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Pesquisa : B03.510.460.400 [Categoria DeCS]
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[PMID]:28215714
[Au] Autor:Roghmann MC; Lydecker AD; Langenberg P; Mongodin EF; Johnson JK
[Ad] Endereço:Geriatrics Research Education and Clinical Center, VA Maryland Health Care System, Baltimore, MD, 21201; Department of Epidemiology and Public Health, University of Maryland School of Medicine, Baltimore, MD, 21201. Electronic address: mroghman@epi.umaryland.edu.
[Ti] Título:Microbiological effect of mupirocin and chlorhexidine for Staphylococcus aureus decolonization in community and nursing home based adults.
[So] Source:Diagn Microbiol Infect Dis;88(1):53-57, 2017 May.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To compare the presence of Staphylococcus aureus and pathogenic Gram-negative rods (GNR) in the anterior nares, posterior pharynx and three skin sites in community-based adults and nursing home-based adults before and after treatment with nasal mupirocin and topical chlorhexidine. METHODS: S. aureus-colonized adults were recruited from the community (n=26) and from nursing homes (n=8). Eligible participants were cultured for S. aureus and GNR during two study visits and then received intranasal mupirocin and topical chlorhexidine for 5days, with a 2-month follow-up period. RESULTS: After decolonization, we found sustained decreases of S. aureus colonization in nose, throat and skin sites over 4-8weeks in both populations. Intranasal mupirocin did not increase GNR colonization in nose or throat. Chlorhexidine did not decrease GNR colonization in skin sites. CONCLUSIONS: Decolonization with mupirocin and chlorhexidine leads to a sustained effect on S. aureus colonization without affecting GNR colonization.
[Mh] Termos MeSH primário: Antibacterianos/administração & dosagem
Portador Sadio/tratamento farmacológico
Portador Sadio/microbiologia
Clorexidina/administração & dosagem
Mupirocina/administração & dosagem
Infecções Estafilocócicas/tratamento farmacológico
Staphylococcus aureus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Administração Tópica
Adulto
Idoso
Idoso de 80 Anos ou mais
Infecções Comunitárias Adquiridas/tratamento farmacológico
Infecções Comunitárias Adquiridas/microbiologia
Infecção Hospitalar/tratamento farmacológico
Infecção Hospitalar/microbiologia
Feminino
Infecções por Bactérias Gram-Negativas/microbiologia
Bacilos Gram-Positivos Asporogênicos/efeitos dos fármacos
Bacilos Gram-Positivos Asporogênicos/isolamento & purificação
Seres Humanos
Masculino
Meia-Idade
Nariz/microbiologia
Casas de Saúde
Faringe/microbiologia
Estudos Prospectivos
Pele/microbiologia
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/isolamento & purificação
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); D0GX863OA5 (Mupirocin); R4KO0DY52L (Chlorhexidine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


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[PMID]:28086066
[Au] Autor:Prakash O; Muduli S; Kumar R; Kumari C; Nimonkar Y; Shouche YS; Sharma R
[Ad] Endereço:1​Microbial Culture Collection, National Centre for Cell Science, Pune 411007, Maharashtra, India.
[Ti] Título:Description of Auricoccus indicus gen. nov., sp. nov., isolated from skin of human ear.
[So] Source:Int J Syst Evol Microbiol;67(5):1212-1218, 2017 May.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-positive, non-motile, non-spore-forming, small spherical bacterium, strain S31T, was isolated from skin surface (external ear lobe) of a healthy human subject and characterized using a polyphasic approach. On the basis of 1507 bp 16S rRNA gene sequence comparison, S31T showed highest (92.8 %, AY119686) sequence similarity with Macrococcus brunensis CCUG 47200T followed by Macrococcus caseolyticus DSM 20597T (92.7 % AP009484) and formed a separate clade with 65 % bootstrap support. The DNA G+C content was found to be 34 mol%. Anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 are the predominant fatty acids in fatty acid methyl ester (FAME) profile of strain S31T. It contained A3α type peptidoglycan with l-Lys-Gly3-l-Ala peptide. Comparative study of morphological and physiological traits indicated that S31T has phenetically diverged from its closest relatives. On the basis of morphological, chemotaxonomic and genotypic data, S31T showed marked distinctions from its closest relatives of the family Staphylococcaceae and is proposed to represent a novel genus Auricoccus with Auricoccus indicus as type species of the genus. S31T (CCUG 69858T=KCTC 33611T=MCC 3027T) is the type strain of the species.
[Mh] Termos MeSH primário: Orelha/microbiologia
Bacilos Gram-Positivos Asporogênicos/classificação
Filogenia
Pele/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Bacilos Gram-Positivos Asporogênicos/genética
Bacilos Gram-Positivos Asporogênicos/isolamento & purificação
Seres Humanos
Peptidoglicano/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170114
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001787


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[PMID]:27247386
[Au] Autor:Florea M; Hagemann H; Santosa G; Abbott J; Micklem CN; Spencer-Milnes X; de Arroyo Garcia L; Paschou D; Lazenbatt C; Kong D; Chughtai H; Jensen K; Freemont PS; Kitney R; Reeve B; Ellis T
[Ad] Endereço:Centre for Synthetic Biology and Innovation, Imperial College London, London SW7 2AZ, United Kingdom; Department of Life Sciences, Imperial College London, London SW7 2AZ, United Kingdom;
[Ti] Título:Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain.
[So] Source:Proc Natl Acad Sci U S A;113(24):E3431-40, 2016 06 14.
[Is] ISSN:1091-6490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial cellulose is a strong and ultrapure form of cellulose produced naturally by several species of the Acetobacteraceae Its high strength, purity, and biocompatibility make it of great interest to materials science; however, precise control of its biosynthesis has remained a challenge for biotechnology. Here we isolate a strain of Komagataeibacter rhaeticus (K. rhaeticus iGEM) that can produce cellulose at high yields, grow in low-nitrogen conditions, and is highly resistant to toxic chemicals. We achieved external control over its bacterial cellulose production through development of a modular genetic toolkit that enables rational reprogramming of the cell. To further its use as an organism for biotechnology, we sequenced its genome and demonstrate genetic circuits that enable functionalization and patterning of heterologous gene expression within the cellulose matrix. This work lays the foundations for using genetic engineering to produce cellulose-based materials, with numerous applications in basic science, materials engineering, and biotechnology.
[Mh] Termos MeSH primário: Celulose
Bacilos Gram-Positivos Asporogênicos
Engenharia Metabólica/métodos
[Mh] Termos MeSH secundário: Celulose/biossíntese
Celulose/genética
Bacilos Gram-Positivos Asporogênicos/genética
Bacilos Gram-Positivos Asporogênicos/isolamento & purificação
Bacilos Gram-Positivos Asporogênicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
9004-34-6 (Cellulose)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160602
[St] Status:MEDLINE
[do] DOI:10.1073/pnas.1522985113


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[PMID]:27064548
[Au] Autor:Tang M; Tan L; Wu H; Dai S; Li T; Chen C; Li J; Fan J; Xiang W; Li X; Wang G
[Ad] Endereço:1​ Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Key Laboratory of Marine Materia Medica (LMMM-GD), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China 2​ University of Chinese Academy of Sciences, Beijing 100049, PR Chi
[Ti] Título:Gelatiniphilus marinus gen. nov., sp. nov., a bacterium from the culture broth of a microalga, Picochlorum sp. 122, and emended description of the genus Hwangdonia.
[So] Source:Int J Syst Evol Microbiol;66(8):2893-8, 2016 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped bacterium, designated strain GYP-24T, was isolated from the culture broth of a marine microalga, Picochlorum sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain GYP-24T forms a robust cluster with H.wangdoniaseohaensis KCTC 32177T (95.8 % sequence similarity) in the family Flavobacteriaceae. Growth of strain GYP-24T was observed at 15, 22, 28, 30, 33 and 37 °C (optimal 30-33 °C), pH 6.0-10.0 (optimal pH 7.0-8.0) and in the presence of 0.5-4 % (w/v) NaCl (optimal 2-3 %). The only menaquinone of strain GYP-24T was MK-6, and the G+C content of the genomic DNA was 36.9 mol%. The major fatty acid profile comprised iso-C17 : 0 3-OH, summed feature 3 (C16 : 1 ω7c/ω6c), iso-C15 : 1 G and iso-C15 : 0. The major polar lipids of strain GYP-24T were phosphatidylethanolamine, one unidentified phospholipid, three unidentified aminolipids and three unidentified lipids. Comprehensive analyses based on polyphasic characterization of GYP-24T indicated that it represents a novel species of a new genus, for which the name Gelatiniphilus marinus gen. nov., sp. nov. is proposed. The type strain is GYP-24T (=KCTC 42903T=MCCC 1K01730T). An emended description of the genus Hwangdonia is also given.
[Mh] Termos MeSH primário: Clorófitas/microbiologia
Flavobacteriaceae/classificação
Bacilos Gram-Positivos Asporogênicos/classificação
Microalgas/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Flavobacteriaceae/genética
Flavobacteriaceae/isolamento & purificação
Bacilos Gram-Positivos Asporogênicos/genética
Bacilos Gram-Positivos Asporogênicos/isolamento & purificação
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 71ANL51TLA (menaquinone 6)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160412
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001074


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[PMID]:25157080
[Au] Autor:Yokoyama H; Yamashita T; Morioka R; Ohmori H
[Ad] Endereço:Animal Waste Management and Environment Division, National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan hiroshiy@affrc.go.jp.
[Ti] Título:Extracellular secretion of noncatalytic plant cell wall-binding proteins by the cellulolytic thermophile Caldicellulosiruptor bescii.
[So] Source:J Bacteriol;196(21):3784-92, 2014 Nov.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Caldicellulosiruptor bescii efficiently degrades cellulose, xylan, and native grasses at high temperatures above 70°C under anaerobic conditions. C. bescii extracellularly secretes multidomain glycoside hydrolases along with proteins of unknown function. In this study, we analyzed the C. bescii proteins that bind to the cell walls of timothy grass by using mass spectrometry, and we identified four noncatalytic plant cell wall-binding proteins (PWBPs) with high pI values (9.2 to 9.6). A search of a conserved domain database showed that these proteins possess a common domain related to solute-binding proteins. In addition, 12 genes encoding PWBP-like proteins were detected in the C. bescii genomic sequence. To analyze the binding properties of PWBPs, recombinant PWBP57 and PWBP65, expressed in Escherichia coli, were prepared. The PWBPs displayed a wide range of binding specificities: they bound to cellulose, lichenan, xylan, arabinoxylan, glucuronoxylan, mannan, glucomannan, pectin, oligosaccharides, and the cell walls of timothy grass. The proteins showed the highest binding affinity for the plant cell wall, with association constant (Ka) values of 5.2 × 10(6) to 44 × 10(6) M(-1) among the insoluble polysaccharides tested, as measured using depletion binding isotherms. Affinity gel electrophoresis demonstrated that the proteins bound to the acidic polymer pectin most strongly among the soluble polysaccharides tested. Fluorescence microscopic analysis showed that the proteins bound preferentially to the cell wall in a section of grass leaf. Binding of noncatalytic PWBPs with high pI values might be necessary for efficient utilization of polysaccharides by C. bescii at high temperatures.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Parede Celular/metabolismo
Celulose/metabolismo
Regulação Bacteriana da Expressão Gênica/fisiologia
Bacilos Gram-Positivos Asporogênicos/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Clonagem Molecular
Filogenia
Células Vegetais/metabolismo
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 9004-34-6 (Cellulose)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:150804
[Lr] Data última revisão:
150804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140827
[St] Status:MEDLINE
[do] DOI:10.1128/JB.01897-14


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[PMID]:24824639
[Au] Autor:Sizova MV; Muller PA; Stancyk D; Panikov NS; Mandalakis M; Hazen A; Hohmann T; Doerfert SN; Fowle W; Earl AM; Nelson KE; Epstein SS
[Ad] Endereço:Department of Biology, Northeastern University, Boston, MA 02115, USA s.epstein@neu.edu m.sizova@neu.edu.
[Ti] Título:Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov., obligately anaerobic bacteria from the human oral cavity, and emended description of the genus Oribacterium.
[So] Source:Int J Syst Evol Microbiol;64(Pt 8):2642-9, 2014 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Three strictly anaerobic, Gram-positive, non-spore-forming, rod-shaped, motile bacteria, designated strains ACB1(T), ACB7(T) and ACB8, were isolated from human subgingival dental plaque. All strains required yeast extract for growth. Strains ACB1(T) and ACB8 were able to grow on glucose, lactose, maltose, maltodextrin and raffinose; strain ACB7(T) grew weakly on sucrose only. The growth temperature range was 30-42 °C with optimum growth at 37 °C. Major metabolic fermentation end products of strain ACB1(T) were acetate and lactate; the only product of strains ACB7(T) and ACB8 was acetate. Major fatty acids of strain ACB1(T) were C(14 : 0), C(16 : 0), C(16 : 1)ω7c dimethyl aldehyde (DMA) and C(18 : 1)ω7c DMA. Major fatty acids of strain ACB7(T) were C(12 : 0), C(14 : 0), C(16 : 0), C(16 : 1)ω7c and C(16 : 1)ω7c DMA. The hydrolysate of the peptidoglycan contained meso-diaminopimelic acid, indicating peptidoglycan type A1γ. Genomic DNA G+C content varied from 42 to 43.3% between strains. According to 16S rRNA gene sequence phylogeny, strains ACB1(T), ACB8 and ACB7(T) formed two separate branches within the genus Oribacterium, with 98.1-98.6% sequence similarity to the type strain of the type species, Oribacterium sinus. Predicted DNA-DNA hybridization values between strains ACB1(T), ACB8, ACB7(T) and O. sinus F0268 were <70%. Based on distinct genotypic and phenotypic characteristics, strains ACB1(T) and ACB8, and strain ACB7(T) are considered to represent two distinct species of the genus Oribacterium, for which the names Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov. are proposed. The type strains are ACB1(T) ( = DSM 24637(T) = HM-481(T) = ATCC BAA-2638(T)) and ACB7(T) ( = DSM 24638(T) = HM-482(T) = ATCC BAA-2639(T)), respectively.
[Mh] Termos MeSH primário: Placa Dentária/microbiologia
Bacilos Gram-Positivos Asporogênicos/classificação
Boca/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Adulto
Bactérias Anaeróbias/classificação
Bactérias Anaeróbias/genética
Bactérias Anaeróbias/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Feminino
Bacilos Gram-Positivos Asporogênicos/genética
Bacilos Gram-Positivos Asporogênicos/isolamento & purificação
Seres Humanos
Dados de Sequência Molecular
Hibridização de Ácido Nucleico
Peptidoglicano/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (RNA, Ribosomal, 16S); 583-93-7 (Diaminopimelic Acid)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140515
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.060988-0


  7 / 175 MEDLINE  
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[PMID]:24425743
[Au] Autor:Horino H; Fujita T; Tonouchi A
[Ad] Endereço:Faculty of Agriculture and Life Science, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan.
[Ti] Título:Description of Anaerobacterium chartisolvens gen. nov., sp. nov., an obligately anaerobic bacterium from Clostridium rRNA cluster III isolated from soil of a Japanese rice field, and reclassification of Bacteroides cellulosolvens Murray et al. 1984 as Pseudobacteroides cellulosolvens gen. nov., comb. nov.
[So] Source:Int J Syst Evol Microbiol;64(Pt 4):1296-303, 2014 Apr.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An obligately anaerobic bacterial strain designated T-1-35(T) was isolated as a dominant cultivable cellulose-degrading bacterium from soil of a Japanese rice field as an anaerobic filter-paper degrader. Cells of strain T-1-35(T) stained Gram-positive and were non-spore-forming rods with rounded ends, 0.8-1.0×3.5-15.0 µm, and motile by means of two to four polar flagella. Cells of strain T-1-35(T) exhibited pleomorphism: in aged cultures (over 90 days of incubation), almost all cells were irregularly shaped. Although no spore formation was observed, cells tolerated high temperatures, up to 90 °C for 10 min. The temperature range for growth was 15-40 °C, with an optimum at 35 °C. The pH range for growth was 5.5-9.0, with an optimum at pH 8.0-8.5 (slightly alkaliphilic). Strain T-1-35(T) fermented some carbohydrates to produce ethanol and lactate as the major products. Major cellular fatty acids were iso-C16 : 0 and iso-C13 : 0 3-OH. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain T-1-35(T) belonged to Clostridium rRNA cluster III. The closest relative of strain T-1-35(T) was Bacteroides cellulosolvens WM2(T), with 16S rRNA gene sequence similarity of 93.4 %. Phenotypic, physiological and molecular genetic methods demonstrated that strain T-1-35(T) was distinct from its phylogenetic relatives (members of Clostridium rRNA cluster III) because it predominantly produced ethanol, iso-C13 : 0 3-OH was a major cellular fatty acid and it always exhibited pleomorphism. On the basis of the results of a polyphasic taxonomic study, strain T-1-35(T) is considered to represent a novel genus and species, Anaerobacterium chartisolvens gen. nov., sp. nov. The type strain of Anaerobacterium chartisolvens is T-1-35(T) ( = DSM 27016(T) = NBRC 109520(T)). In addition, from the results of our phylogenetic analysis and its phenotypic features, the species Bacteroides cellulosolvens Murray et al. 1984 is proposed to be reclassified in the new genus Pseudobacteroides as Pseudobacteroides cellulosolvens gen. nov., comb. nov., with the type strain WM2(T) ( = ATCC 35603(T) = DSM 2933(T) = NRCC 2944(T)).
[Mh] Termos MeSH primário: Bacilos Gram-Positivos Asporogênicos/classificação
Oryza/microbiologia
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Bactérias Anaeróbias/classificação
Bactérias Anaeróbias/genética
Bactérias Anaeróbias/isolamento & purificação
Técnicas de Tipagem Bacteriana
Bacteroides/classificação
Composição de Bases
Celulose/metabolismo
DNA Bacteriano/genética
Ácidos Graxos/química
Bacilos Gram-Positivos Asporogênicos/genética
Bacilos Gram-Positivos Asporogênicos/isolamento & purificação
Japão
Dados de Sequência Molecular
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 9004-34-6 (Cellulose)
[Em] Mês de entrada:1405
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140116
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.059378-0


  8 / 175 MEDLINE  
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[PMID]:23625266
[Au] Autor:Takada T; Kurakawa T; Tsuji H; Nomoto K
[Ad] Endereço:Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo, 186-8650, Japan.
[Ti] Título:Fusicatenibacter saccharivorans gen. nov., sp. nov., isolated from human faeces.
[So] Source:Int J Syst Evol Microbiol;63(Pt 10):3691-6, 2013 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Three Gram-stain-positive, obligately anaerobic, non-motile, non-spore-forming, spindle-shaped bacterial strains (HT03-11(T), KO-38 and TT-111), isolated from human faeces were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing showed that the strains were highly related to each other genetically (displaying >99 % sequence similarity) and represented a previously unknown subline within the Blautia coccoides rRNA group of organisms (cluster XIVa). The closest phylogenetic neighbours of strain HT03-11(T) were Clostridium bolteae WAL 16351(T) (93.7 % 16S rRNA gene sequence similarity) and Clostridium saccharolyticum WM1(T) (93.7 % similarity). All isolates produced lactic acid, formic acid, acetic acid and succinic acid as fermentation end products from glucose. Their chemotaxonomic properties included lysine as the cell wall diamino acid and C16 : 0, C18 : 1ω7c DMA and C16 : 0 DMA as the major fatty acids. The G+C contents of the genomic DNA were 46.9-47.2 mol% (HPLC). Several phenotypic and chemotaxonomic characteristics could be readily used to differentiate the isolates from phylogenetically related clostridia. Therefore, strains HT03-11(T), KO-38 and TT-111 represent a novel species in a new genus of the family Lachnospiraceae, for which the name Fusicatenibacter saccharivorans gen. nov., sp. nov. is proposed. The type strain of the type species is HT03-11(T) ( = YIT 12554(T) = JCM 18507(T) = DSM 26062(T)).
[Mh] Termos MeSH primário: Fezes/microbiologia
Bacilos Gram-Positivos Asporogênicos/classificação
Filogenia
[Mh] Termos MeSH secundário: Adulto
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/análise
Bacilos Gram-Positivos Asporogênicos/genética
Bacilos Gram-Positivos Asporogênicos/isolamento & purificação
Seres Humanos
Lisina/análise
Meia-Idade
Dados de Sequência Molecular
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1312
[Cu] Atualização por classe:131007
[Lr] Data última revisão:
131007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130430
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.045823-0


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[PMID]:23024143
[Au] Autor:Fraj B; Ben Hania W; Postec A; Hamdi M; Ollivier B; Fardeau ML
[Ad] Endereço:Aix-Marseille University, Mediterranean Institute of Oceanography (MIO), IRD, MIO, UMR235 CNRS/INSU, MIO, UMR 7294, 13288 Marseille Cedex 9, France.
[Ti] Título:Fonticella tunisiensis gen. nov., sp. nov., isolated from a hot spring.
[So] Source:Int J Syst Evol Microbiol;63(Pt 6):1947-50, 2013 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A strictly anaerobic, moderately thermophilic, halotolerant rod, designated BELH25(T), was isolated from a water sample of a Tunisian hot spring. Cells were non-motile, 2-6 µm long and 0.4-0.6 µm wide, appearing singly or in pairs. The isolate grew at 45-70 °C (optimum 55 °C), at pH 6.2-8.0 (optimum pH 7.0) and with 0-4% NaCl (optimum 0-2.0%). Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Strain BELH25(T) used cellobiose, fructose, galactose, glucose, maltose, mannose, sucrose, starch and yeast extract as electron donors. The main fermentation products from glucose metabolism were formate, acetate, ethanol and CO2. The predominant cellular fatty acids were iso-C15:0, iso-C17:0 and anteiso-C15:0. The DNA G+C content was 37.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain BELH25(T) was most closely related to Caloramator viterbiensis JW/MS-VS5(T) and Fervidicella metallireducens AeB(T) (92.2 and 92.1% sequence similarity, respectively), and the isolate was positioned approximately equidistantly between these genera. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, strain BELH25(T) is proposed to be a member of a novel species of a novel genus within the order Clostridiales, family Clostridiaceae, for which the name Fonticella tunisiensis gen. nov., sp. nov. is proposed. The type strain of the type species is BELH25(T) (=DSM 24455(T)=JCM 17559(T)).
[Mh] Termos MeSH primário: Bacilos Gram-Positivos Asporogênicos/classificação
Fontes Termais/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/análise
Bacilos Gram-Positivos Asporogênicos/genética
Bacilos Gram-Positivos Asporogênicos/isolamento & purificação
Dados de Sequência Molecular
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Tunísia
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1309
[Cu] Atualização por classe:130610
[Lr] Data última revisão:
130610
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121002
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.041947-0


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[PMID]:22039006
[Au] Autor:Jiang F; Cao SJ; Li ZH; Fan H; Li HF; Liu WJ; Yuan HL
[Ad] Endereço:College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
[Ti] Título:Salisediminibacterium halotolerans gen. nov., sp. nov., a halophilic bacterium from soda lake sediment.
[So] Source:Int J Syst Evol Microbiol;62(Pt 9):2127-32, 2012 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An orange-pigmented, Gram-reaction-positive, non-spore-forming, halophilic, alkali-tolerant rod, designated strain halo-2(T), was isolated from sediment of Xiarinaoer soda lake, in China's Inner Mongolia Autonomous Region. Strain halo-2(T) grew in a complex medium with 3-30 % (w/v) NaCl and at pH 5-10. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was MK-7. The predominant cellular fatty acids were anteiso-C(15 : 0) (43.6 %), anteiso-C(17 : 0) (14.8 %) and iso-C(15 : 0) (6.8 %) and the polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content of the novel strain was 48.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain halo-2(T) was most closely related to Bacillus agaradhaerens DSM 8721(T) (93.9 % sequence similarity). However, strain halo-2(T) could be clearly differentiated from its closest phylogenetic relatives on the basis of several phenotypic, genotypic and chemotaxonomic characteristics. Strain halo-2(T) therefore represents a novel species in a new genus for which the name Salisediminibacterium halotolerans gen. nov., sp. nov. is proposed. The type strain of the type species is halo-2(T) (= CGMCC 1.7654(T) = NBRC 104935(T)).
[Mh] Termos MeSH primário: Bacilos Gram-Positivos Asporogênicos/classificação
Lagos/microbiologia
Filogenia
Microbiologia da Água
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/análise
Sedimentos Geológicos/microbiologia
Bacilos Gram-Positivos Asporogênicos/genética
Bacilos Gram-Positivos Asporogênicos/isolamento & purificação
Dados de Sequência Molecular
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1212
[Cu] Atualização por classe:120910
[Lr] Data última revisão:
120910
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111101
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.034488-0



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