Base de dados : MEDLINE
Pesquisa : B03.510.460.400.410 [Categoria DeCS]
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[PMID]:23764091
[Au] Autor:Kloesel B; Beliveau M; Patel R; Trousdale RT; Sia IG
[Ti] Título:Bulleidia extructa periprosthetic hip joint infection, United States.
[So] Source:Emerg Infect Dis;19(7):1170-1, 2013 Jul.
[Is] ISSN:1080-6059
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Bacilos Gram-Positivos Asporogênicos Regulares/isolamento & purificação
Infecções por Bactérias Gram-Positivas/diagnóstico
Prótese de Quadril/efeitos adversos
Infecções Relacionadas à Prótese/diagnóstico
[Mh] Termos MeSH secundário: Idoso de 80 Anos ou mais
Artroplastia de Quadril
Terapia Combinada
Infecções por Bactérias Gram-Positivas/microbiologia
Seres Humanos
Masculino
Infecções Relacionadas à Prótese/tratamento farmacológico
Infecções Relacionadas à Prótese/cirurgia
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1401
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130615
[St] Status:MEDLINE
[do] DOI:10.3201/eid1907.130078


  2 / 8 MEDLINE  
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[PMID]:19446351
[Au] Autor:Terrade N; Mira de Orduña R
[Ad] Endereço:Department of Food Science, University of Guelph, Canada.
[Ti] Título:Determination of the essential nutrient requirements of wine-related bacteria from the genera Oenococcus and Lactobacillus.
[So] Source:Int J Food Microbiol;133(1-2):8-13, 2009 Jul 31.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Wine lactic acid bacteria (LAB) are responsible for the malolactic fermentation (MLF) in wine production. Wine LAB have fastidious nutrient requirements but their auxotrophies remain little studied. The ability of specific wine nutrients to meet the nutritional requirements of wine LAB, and thus support MLF, remains unclear. This work investigated the essential growth requirements of four strains of wine LAB from the genera Oenococcus and Lactobacillus using the single omission technique with a suitable chemically defined medium. For the determination of auxotrophies, at least 3 (and up to 15) subcultures in deficient media were made, and intra- and extracellular nutrient carry over was reduced by small inoculation rates and washing cells 3 times between transfers. This careful methodology revealed more auxotrophies than those described for wine LAB in the literature. The essential bacterial nutrient requirements were found to be strain specific. 10 compounds were essential for all wine LAB tested, the carbon and phosphate source, manganese, as well as several amino acids (proline, arginine and the branched amino acids valine, leucine and isoleucine) and vitamins (nicotinic acid and pantothenic acids). Nucleotides were not essential for any of the bacteria studied. The two Oenococcus oeni strains revealed a larger number of auxotrophies (18 and 21) and had a higher degree of nutritional similarity (86%) defined as percentage of common requirements per maximum total requirements. The two Lactobacillus strains only had 11 and 14 auxotrophies and the similarity was 79%, but both were auxotroph for riboflavin, which was not needed by the O. oeni strains. Data on the common requirements may be used to further study the ability of wines or commercial nutrients to support MLF and to consider the microbiological stability of finished wines. The results indicate that absence of riboflavin in oenological nutrient preparations may allow to create a specific advantage for indigenous or inoculated O. oeni, which are generally desired for MLF.
[Mh] Termos MeSH primário: Microbiologia de Alimentos
Bacilos Gram-Positivos Asporogênicos Regulares/crescimento & desenvolvimento
Lactobacillus/crescimento & desenvolvimento
Malato Desidrogenase/metabolismo
Vinho/microbiologia
[Mh] Termos MeSH secundário: Aminoácidos
Carbono
Contagem de Colônia Microbiana
Meios de Cultura
Fermentação
Leuconostoc/crescimento & desenvolvimento
Manganês
Fosfatos
Complexo Vitamínico B
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amino Acids); 0 (Culture Media); 0 (Phosphates); 12001-76-2 (Vitamin B Complex); 42Z2K6ZL8P (Manganese); 7440-44-0 (Carbon); EC 1.1.1.- (malolactic enzyme); EC 1.1.1.37 (Malate Dehydrogenase)
[Em] Mês de entrada:0912
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090519
[St] Status:MEDLINE
[do] DOI:10.1016/j.ijfoodmicro.2009.03.020


  3 / 8 MEDLINE  
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[PMID]:11016696
[Au] Autor:Grayson TH; Alexander SM; Cooper LF; Gilpin ML
[Ad] Endereço:Department of Biological Sciences, University of Plymouth, Devon, UK. tgrayson@plymouth.ac.uk
[Ti] Título:Renibacterium salmoninarum isolates from different sources possess two highly conserved copies of the rRNA operon .
[So] Source:Antonie Van Leeuwenhoek;78(1):51-61, 2000 Jul.
[Is] ISSN:0003-6072
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The nucleotide sequences of the rRNA genes and the 5' flanking region were determined for R. salmoninarum ATCC 33209T from overlapping products generated by PCR amplification from the genomic DNA. Comparison of the sequences with rRNA genes from a variety of bacteria demonstrated the close relatedness between R. salmoninarum and the high G+C group of the actinobacteria, in particular, Arthrobacter species. A regulatory element within the 5' leader of the rRNA operon was identical to an element, CL2, described for mycobacteria. PCR, DNA sequence analysis, and DNA hybridisation were performed to examine variation between isolates from diverse sources which represented the four 16S-23S rRNA intergenic spacer sequevars previously described for R. salmoninarum. Two 23S-5S rRNA intergenic spacer sequevars of identical length were found. DNA hybridisation using probes complementary to 23S rDNA and 16S rDNA identified two rRNA operons which were identical or nearly identical amongst 40 isolates sourced from a variety of countries.
[Mh] Termos MeSH primário: Sequência Conservada
DNA Ribossômico/genética
Bacilos Gram-Positivos Asporogênicos Regulares/genética
Óperon
RNA Ribossômico/genética
[Mh] Termos MeSH secundário: Actinomycetales/genética
Sequência de Bases
Dosagem de Genes
Variação Genética
Dados de Sequência Molecular
Filogenia
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
RNA Ribossômico 23S/genética
RNA Ribossômico 5S/genética
Mapeamento por Restrição
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Ribosomal); 0 (RNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 0 (RNA, Ribosomal, 23S); 0 (RNA, Ribosomal, 5S)
[Em] Mês de entrada:0101
[Cu] Atualização por classe:081121
[Lr] Data última revisão:
081121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:001004
[St] Status:MEDLINE


  4 / 8 MEDLINE  
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[PMID]:10950180
[Au] Autor:Pascho RJ; Ongerth JE
[Ad] Endereço:US Geological Survey, Biological Resources Division, Western Fisheries Research Center, Seattle, Washington 98115, USA. ron_pascho@usgs.gov
[Ti] Título:Method for flow cytometric monitoring of Renibacterium salmoninarum inactivation.
[So] Source:Dis Aquat Organ;41(3):181-93, 2000 Jul 14.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The slow growth of Renibacterium salmoninarum limits the usefulness of culture as a research tool. Development of a 2-color flow cytometric assay to quantify the proportions of live and dead R. salmoninarum in a test population is described. Bacteria were simultaneously stained with fluorescein isothiocyanate-conjugated immunoglobulin and exposed to the exclusion dye propidium iodide. Propidium iodide red fluorescence profiles of control groups of untreated and killed R. salmoninarum were compared with those for bacteria exposed to chlorine. Bacterial inactivation was based on mean red fluorescence intensity, and analyzed by high-red fluorescence intensity (HRFI) and curve subtraction (CS) analyses. When the concentration of R. salmoninarum was 8.65 x 10(6) bacteria ml(-1) and the bacteria exposed to chlorine at 1 mg l(-1) for periods from 1 to 20 min (high-Rs assessment), the mean red fluorescence intensity of the profile for each chlorine-exposure group was higher than that for the untreated control (p < 0.0001). When the concentration of R. salmoninarum was reduced to 1.76 x 10(6) bacteria ml(-1) and exposed to 0.8 mg l(-1) free chlorine level for periods from 20 s to 5 min (reduced-Rs assessment), the mean red fluorescence intensities of the exposure groups were higher than that for the untreated control only when the R. salmoninarum was exposed to chlorine for at least 1 min (p < or = 0.01). On the basis of red fluorescence intensity, the proportion of dead cells generally increased with the duration of chlorine exposure. Whereas the rates of inactivation derived from the HRFI and CS analyses did not correlate with the duration of exposure in the high-Rs assessment (r2 < or = 0.27), there was a correlation between these estimates and the duration of exposure in the reduced-Rs assessment (r2 > or = 0.92). Because of the rapid loss of culturable R. salmoninarum in both assessments following chlorine exposure, neither the duration of exposure nor the inactivation estimates correlated with bacteriological culture (r2 < or = 0.22). In both assessments, there was a correlation between the estimates of inactivation based upon HRFI and CS analyses (r2 > 0.99). These results suggest that flow cytometry can be used as a supplementary or alternative method to bacteriological culture for monitoring the inactivation of R. salmoninarum.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas/veterinária
Citometria de Fluxo/veterinária
Bacilos Gram-Positivos Asporogênicos Regulares/isolamento & purificação
[Mh] Termos MeSH secundário: Cloro/farmacologia
Bacilos Gram-Positivos Asporogênicos Regulares/efeitos dos fármacos
Temperatura Alta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
4R7X1O2820 (Chlorine)
[Em] Mês de entrada:0009
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:000819
[St] Status:MEDLINE


  5 / 8 MEDLINE  
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[PMID]:10698783
[Au] Autor:Verschuere L; Heang H; Criel G; Sorgeloos P; Verstraete W
[Ad] Endereço:Laboratory of Microbial Ecology and Technology, Department of Biochemical and Microbiological Technology, University of Ghent, B-9000 Ghent, Belgium.
[Ti] Título:Selected bacterial strains protect Artemia spp. from the pathogenic effects of Vibrio proteolyticus CW8T2.
[So] Source:Appl Environ Microbiol;66(3):1139-46, 2000 Mar.
[Is] ISSN:0099-2240
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study Vibrio proteolyticus CW8T2 has been identified as a virulent pathogen for Artemia spp. Its infection route has been visualized with transmission electron microscopy. The pathogen affected microvilli and gut epithelial cells, disrupted epithelial cell junctions, and reached the body cavity, where it devastated cells and tissues. In vivo antagonism tests showed that preemptive colonization of the culture water with nine selected bacterial strains protected Artemia juveniles against the pathogenic effects. Two categories of the selected strains could be distinguished: (i) strains providing total protection, as no mortality occurred 2 days after the experimental infection with V. proteolyticus CW8T2, with strain LVS8 as a representative, and (ii) strains providing partial protection, as significant but not total mortality was observed, with strain LVS2 as a representative. The growth of V. proteolyticus CW8T2 in the culture medium was slowed down in the presence of strains LVS2 and LVS8, but growth suppression was distinctly higher with LVS8 than with LVS2. It was striking that the strains that gave only partial protection against the pathogen in the in vivo antagonism test showed also a restricted capability to colonize the Artemia compared to the strains providing total protection. The in vivo antagonism tests and the filtrate experiments showed that probably no extracellular bacterial compounds were involved in the protective action but that the living cells were required to protect Artemia against V. proteolyticus CW8T2.
[Mh] Termos MeSH primário: Antibiose
Artemia/microbiologia
Bactérias Gram-Negativas/fisiologia
Bacilos Gram-Positivos Asporogênicos Regulares/fisiologia
Vibrio/patogenicidade
[Mh] Termos MeSH secundário: Alcaligenes/fisiologia
Animais
Bacteriólise
Sistema Digestório/microbiologia
Sistema Digestório/patologia
Células Epiteliais/microbiologia
Células Epiteliais/patologia
Moraxella/fisiologia
Vibrionaceae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:0004
[Cu] Atualização por classe:140615
[Lr] Data última revisão:
140615
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:000304
[St] Status:MEDLINE


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[PMID]:10574090
[Au] Autor:Rattanasomboon N; Bellara SR; Harding CL; Fryer PJ; Thomas CR; Al-Rubeai M; McFarlane CM
[Ad] Endereço:School of Chemical Engineering, University of Birmingham, Edgbaston, UK.
[Ti] Título:Growth and enumeration of the meat spoilage bacterium Brochothrix thermosphacta.
[So] Source:Int J Food Microbiol;51(2-3):145-58, 1999 Oct 15.
[Is] ISSN:0168-1605
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Brochothrix thermosphacta is a common meat spoilage bacterium. The morphology of this bacterium changes from coccobacilli and short rods to chains during growth, which may give a false estimation in numbers using some enumeration techniques. Methods for the quantification of this bacterium have been compared. Turbidimetric readings showed good agreement with cell dry weight indicating that the former provides a good measure of the change in cell mass during growth. The turbidimetric method also correlated well with bacterial numbers determined by plate counts, flow cytometry and manual counts (by microscope) over a limited range of 10(7)-10(9) cells/ml. Flow cytometry and manual counts gave a linear relationship over a wider range of 10(5)-10(9) cells/ml. The sensitivity of analysis, growth rates and lag time attained using these methods were also compared. As a consequence of changes in bacterial cell size during growth, turbidimetry over-estimated the growth rate. The plate count method proved unable to detect the difference between bacteria existing as chains or single cells. The sensitivity of analysis and the calculated growth related parameters were similar for flow cytometry and manual counts. This suggests that flow cytometry is capable of counting individual cells in a chain. Further investigation showed that passage of B. thermosphacta cells through the flow cytometer resulted in the breakage of chains into single cells. The reliability, low error and rapidity of this technique make it attractive for bacterial enumeration, something which has been demonstrated using B. thermosphacta, a bacterium which exhibits complex morphologies.
[Mh] Termos MeSH primário: Contagem de Colônia Microbiana/métodos
Microbiologia de Alimentos
Bacilos Gram-Positivos Asporogênicos Regulares/crescimento & desenvolvimento
Bacilos Gram-Positivos Asporogênicos Regulares/isolamento & purificação
Produtos da Carne/microbiologia
[Mh] Termos MeSH secundário: Animais
Separação Celular
Citometria de Fluxo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:9912
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:991126
[St] Status:MEDLINE


  7 / 8 MEDLINE  
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[PMID]:10084700
[Au] Autor:Flesch IE; Kaufmann SH
[Ad] Endereço:Department of Immunology, University Clinics Ulm, Germany. inge.flesch@medizin.uni.ulm.de
[Ti] Título:Effect of fetal calf serum on cytokine release by bone marrow-derived macrophages during infection with intracellular bacteria.
[So] Source:Immunobiology;200(1):120-7, 1999 Feb.
[Is] ISSN:0171-2985
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bone marrow-derived macrophages (BMM) comprise a population of quiescent cells which can be activated by defined signals. Here, we directly compare the release of chemokines and monokines by BMM raised either in serum-supplemented or in serum-free medium in response to Listeria monocytogenes EGD or Mycobacterium bovis BCG infection. We focused on this issue because there have been several controversial reports on the production of cytokines by BMM due to different in vitro culture conditions. Culture in serum-supplemented medium primed BMM for release of monocyte chemoattractant protein (MCP)-1, interleukin (IL)-6, and IL-12, but had no effect on macrophage inflammatory protein (MIP)-1alpha and tumor necrosis factor (TNF)-alpha production in response to L. monocytogenes infection. After challenge infection with M. bovis, BMM raised and stimulated in serum-supplemented medium secreted higher levels of MCP-1, MIP-1alpha, IL-6, and TNF-alpha but not of IL-12 as compared to BMM cultured and infected in a serum-free medium. The effects of serum could be partially mimicked by interferon-gamma. Because the serum components responsible for BMM priming are undefined, BMM cultured under serum-free conditions provide an appropriate cell population for defining macrophage activating signals.
[Mh] Termos MeSH primário: Células da Medula Óssea/imunologia
Meios de Cultura
Citocinas/secreção
Bacilos Gram-Positivos Asporogênicos Regulares/imunologia
Ativação de Macrófagos
Macrófagos/imunologia
[Mh] Termos MeSH secundário: Animais
Sangue
Células da Medula Óssea/efeitos dos fármacos
Células da Medula Óssea/microbiologia
Células Cultivadas/efeitos dos fármacos
Quimiocinas/secreção
Meios de Cultura Livres de Soro
Interferon gama/farmacologia
Listeria monocytogenes/imunologia
Macrófagos/efeitos dos fármacos
Macrófagos/microbiologia
Camundongos
Camundongos Endogâmicos C57BL
Monocinas/secreção
Mycobacterium bovis/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemokines); 0 (Culture Media); 0 (Culture Media, Serum-Free); 0 (Cytokines); 0 (Monokines); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:9905
[Cu] Atualização por classe:081121
[Lr] Data última revisão:
081121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:990320
[St] Status:MEDLINE


  8 / 8 MEDLINE  
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[PMID]:9766080
[Au] Autor:Mulet-Powell N; Lacoste-Armynot AM; Viñas M; Simeon de Buochberg M
[Ad] Endereço:Laboratory of Microbiology, Institute of Public Health, University of Barcelona, Spain.
[Ti] Título:Interactions between pairs of bacteriocins from lactic bacteria.
[So] Source:J Food Prot;61(9):1210-2, 1998 Sep.
[Is] ISSN:0362-028X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Activity of pairs of crude extracts of lactic acid bacteria (LAB) containing different bacteriocins (nisin, pediocin AcH, lacticin 481, lactacin F, and lactacin B) was measured against 10 different indicator strains. Experiments were carried out both in liquid and on solid media. Both synergisms and antagonisms were observed. Lacticin 481 produced mainly antagonistic effects whereas pediocin AcH produced mainly synergistic effects. The use of more than one LAB bacteriocin as a combination biopreservative might be envisaged.
[Mh] Termos MeSH primário: Bacteriocinas/farmacologia
Interações Medicamentosas
Cocos Gram-Positivos/metabolismo
Lactobacillus/metabolismo
[Mh] Termos MeSH secundário: Bacteriocinas/metabolismo
Meios de Cultura
Antagonismo de Drogas
Sinergismo Farmacológico
Bacilos Gram-Positivos Asporogênicos Regulares/efeitos dos fármacos
Bacilos Gram-Positivos Asporogênicos Regulares/crescimento & desenvolvimento
Cocos Gram-Positivos/efeitos dos fármacos
Cocos Gram-Positivos/crescimento & desenvolvimento
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacteriocins); 0 (Culture Media)
[Em] Mês de entrada:9812
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:981010
[St] Status:MEDLINE



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