Base de dados : MEDLINE
Pesquisa : B03.585 [Categoria DeCS]
Referências encontradas : 15 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 2 ir para página        

  1 / 15 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28528390
[Au] Autor:Ballhorn DJ; Elias JD; Balkan MA; Fordyce RF; Kennedy PG
[Ad] Endereço:Department of Biology, Portland State University, 1719 SW 10th Ave, Portland, OR, 97201, USA. ballhorn@pdx.edu.
[Ti] Título:Colonization by nitrogen-fixing Frankia bacteria causes short-term increases in herbivore susceptibility in red alder (Alnus rubra) seedlings.
[So] Source:Oecologia;184(2):497-506, 2017 Jun.
[Is] ISSN:1432-1939
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Carbon allocation demands from root-nodulating nitrogen-fixing bacteria (NFB) can modulate the host plant's chemical phenotype, with strong bottom-up effects on herbivores. In contrast to well-studied rhizobia, the effects of other important NFB on plant chemistry and herbivory are much less understood. Here, combining field surveys in the Oregon Coast Range, USA with laboratory experiments, we analyzed how N -fixing Frankia bacteria influenced plant growth, chemistry, and herbivory on Alnus rubra (red alder) seedlings. In the field, we quantified Frankia nodulation, herbivore damage, and plant size. In the laboratory, we grew seedlings with Frankia (F+), Frankia-free but nitrogen-fertilized (N+), or both uncolonized and unfertilized (F-N-) and assessed growth and leaf chemistry. We further conducted choice trials with black slugs, Arion rufus, a natural red alder herbivore. In the field, Frankia nodulation was significantly positively correlated with herbivory and negatively with seedling height. In contrast, in the lab, F+ as well as N+ seedlings were significantly taller than the F-N- controls. Seedlings from both treatments also had significantly increased leaf protein concentration compared to controls, whereas carbon-based nutritive compounds (carbohydrates) as well as leaf palatability-decreasing condensed tannins, lignin, and fiber were decreased in F+ but not in N+ treatments. In the choice assays, slugs preferred leaf material from F+ seedlings, but the effects were only significant in young leaves. Our study indicates that colonization by Frankia causes short-term ecological costs in terms of susceptibility to herbivory. However, the ubiquity of this symbiosis in natural settings suggests that these costs are outweighed by benefits beyond the seedling stage.
[Mh] Termos MeSH primário: Alnus
Frankia
Herbivoria
Bactérias Fixadoras de Nitrogênio
[Mh] Termos MeSH secundário: Nitrogênio
Oregon
Plântulas
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
N762921K75 (Nitrogen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170522
[St] Status:MEDLINE
[do] DOI:10.1007/s00442-017-3888-2


  2 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28362815
[Au] Autor:Brusamarello-Santos LC; Gilard F; Brulé L; Quilleré I; Gourion B; Ratet P; Maltempi de Souza E; Lea PJ; Hirel B
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Federal University of Paraná, Centro Politécnico, Curutiba, Paraná, Brazil.
[Ti] Título:Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense.
[So] Source:PLoS One;12(3):e0174576, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Maize roots can be colonized by free-living atmospheric nitrogen (N2)-fixing bacteria (diazotrophs). However, the agronomic potential of non-symbiotic N2-fixation in such an economically important species as maize, has still not been fully exploited. A preliminary approach to improve our understanding of the mechanisms controlling the establishment of such N2-fixing associations has been developed, using two maize inbred lines exhibiting different physiological characteristics. The bacterial-plant interaction has been characterized by means of a metabolomic approach. Two established model strains of Nif+ diazotrophic bacteria, Herbaspirillum seropedicae and Azospirillum brasilense and their Nif- couterparts defficient in nitrogenase activity, were used to evaluate the impact of the bacterial inoculation and of N2 fixation on the root and leaf metabolic profiles. The two N2-fixing bacteria have been used to inoculate two genetically distant maize lines (FV252 and FV2), already characterized for their contrasting physiological properties. Using a well-controlled gnotobiotic experimental system that allows inoculation of maize plants with the two diazotrophs in a N-free medium, we demonstrated that both maize lines were efficiently colonized by the two bacterial species. We also showed that in the early stages of plant development, both bacterial strains were able to reduce acetylene, suggesting that they contain functional nitrogenase activity and are able to efficiently fix atmospheric N2 (Fix+). The metabolomic approach allowed the identification of metabolites in the two maize lines that were representative of the N2 fixing plant-bacterial interaction, these included mannitol and to a lesser extend trehalose and isocitrate. Whilst other metabolites such as asparagine, although only exhibiting a small increase in maize roots following bacterial infection, were specific for the two Fix+ bacterial strains, in comparison to their Fix- counterparts. Moreover, a number of metabolites exhibited a maize-genotype specific pattern of accumulation, suggesting that the highly diverse maize genetic resources could be further exploited in terms of beneficial plant-bacterial interactions for optimizing maize growth, with reduced N fertilization inputs.
[Mh] Termos MeSH primário: Azospirillum brasilense/metabolismo
Herbaspirillum/metabolismo
Bactérias Fixadoras de Nitrogênio/metabolismo
Nitrogênio/metabolismo
Raízes de Plantas/metabolismo
Raízes de Plantas/microbiologia
Zea mays/metabolismo
Zea mays/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
N762921K75 (Nitrogen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174576


  3 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28142226
[Au] Autor:Rush D; Sinninghe Damsté JS
[Ad] Endereço:Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, and Utrecht University, Den Burg, P.O. Box 59 1790 AB, The Netherlands.
[Ti] Título:Lipids as paleomarkers to constrain the marine nitrogen cycle.
[So] Source:Environ Microbiol;19(6):2119-2132, 2017 Jun.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Global climate is, in part, regulated by the effect of microbial processes on biogeochemical cycling. The nitrogen cycle, in particular, is driven by microorganisms responsible for the fixation and loss of nitrogen, and the reduction-oxidation transformations of bio-available nitrogen. Within marine systems, nitrogen availability is often the limiting factor in the growth of autotrophic organisms, intrinsically linking the nitrogen and carbon cycles. In order to elucidate the state of these cycles in the past, and help envisage present and future variability, it is essential to understand the specific microbial processes responsible for transforming bio-available nitrogen species. As most microorganisms are soft-bodied and seldom leave behind physical fossils in the sedimentary record, recalcitrant lipid biomarkers are used to unravel microbial processes in the geological past. This review emphasises the recent advances in marine nitrogen cycle lipid biomarkers, underlines the missing links still needed to fully elucidate past shifts in this biogeochemically-important cycle, and provides examples of biomarker applications in the geological past.
[Mh] Termos MeSH primário: Cianobactérias/metabolismo
Desnitrificação/fisiologia
Lipídeos/fisiologia
Nitrificação/fisiologia
Fixação de Nitrogênio/fisiologia
Bactérias Fixadoras de Nitrogênio/metabolismo
[Mh] Termos MeSH secundário: Biomarcadores
Clima
Nitrogênio/metabolismo
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biomarkers); 0 (Lipids); N762921K75 (Nitrogen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13682


  4 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28120312
[Au] Autor:Thajudeen J; Yousuf J; Veetil VP; Varghese S; Singh A; Abdulla MH
[Ad] Endereço:Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala, 682016, India.
[Ti] Título:Nitrogen fixing bacterial diversity in a tropical estuarine sediments.
[So] Source:World J Microbiol Biotechnol;33(2):41, 2017 Feb.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Microorganisms play a significant role in biogeochemical cycles, especially in the benthic and pelagic ecosystems. Role of environmental parameters in regulating the diversity, distribution and physiology of these microorganisms in tropical marine environment is not well understood. In this study, we have identified dinitrogen (N ) fixing bacterial communities in the sediments by constructing clone libraries of nitrogenase (nifH) gene from four different stations in the Cochin estuary, along the southeastern Arabian Sea. N fixing bacterial clones revealed that over 20 putative diazotrophs belong to alpha-, beta-, gamma-, delta- and epsilon- proteobacteria and firmicutes. Predominant genera among these were Bradyrhizobium sp. (α-proteobacteria), Dechloromonas sp. (ß-proteobacteria); Azotobactor sp., Teredinibacter sp., Methylobacter sp., Rheinheimera sp. and Marinobacterium sp. (γ-proteobacteria); Desulfobacter sp., Desulfobulbus sp. and Desulfovibrio sp. (δ -proteobacteria); Arcobacter sp. and Sulfurospirillum sp. (ε-proteobacteria). Nostoc sp. was solely identified among the cyanobacterial phylotype. Nitrogen fixing Sulfate reducing bacteria (SRBs) such as Desulfobulbus sp., Desulfovibrio sp., Desulfuromonas sp., Desulfosporosinus sp., Desulfobacter sp., were also observed in the study. Most of the bacterial nifH sequences revealed that the identities of N fixing bacteria were less than 95% similar to that available in the GenBank database, which suggested that the sequences were of novel N fixing microorganisms. Shannon-Weiner diversity index of nifH gene ranged from 2.95 to 3.61, indicating an inflated diversity of N fixing bacteria. Canonical correspondence analysis (CCA) implied positive correlation among nifH diversity, N fixation rate and other environmental variables.
[Mh] Termos MeSH primário: Sedimentos Geológicos/microbiologia
Bactérias Fixadoras de Nitrogênio/classificação
Bactérias Fixadoras de Nitrogênio/isolamento & purificação
Oxirredutases/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Biodiversidade
Biblioteca Gênica
Fixação de Nitrogênio
Bactérias Fixadoras de Nitrogênio/genética
Filogenia
Clima Tropical
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 1.- (Oxidoreductases); EC 1.18.6.1 (nitrogenase reductase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-017-2205-x


  5 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27876549
[Au] Autor:Júnior JQ; Jesus ED; Lisboa FJ; Berbara RL; Faria SM
[Ad] Endereço:Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica, RJ, Brazil. Electronic address: joelquintino@yahoo.com.br.
[Ti] Título:Nitrogen-fixing bacteria and arbuscular mycorrhizal fungi in Piptadenia gonoacantha (Mart.) Macbr.
[So] Source:Braz J Microbiol;48(1):95-100, 2017 Jan - Mar.
[Is] ISSN:1678-4405
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The family Leguminosae comprises approximately 20,000 species that mostly form symbioses with arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing bacteria (NFB). This study is aimed at investigating and confirming the dependence on nodulation and biological nitrogen fixation in the specie Piptadenia gonoacantha (Mart.) Macbr., which belongs to the Piptadenia group. Two consecutive experiments were performed in a greenhouse. The experiments were fully randomized with six replicates and a factorial scheme. For the treatments, the two AMF species and three NFB strains were combined to nodulate P. gonoacantha in addition to the control treatments. The results indicate this species' capacity for nodulation without the AMF; however, the AMF+NFB combinations yielded a considerable gain in P. gonoacantha shoot weight compared with the treatments that only included inoculating with bacteria or AMF. The results also confirm that the treatment effects among the AMF+NFB combinations produced different shoot dry weight/root dry weight ratios. We conclude that AMF is not necessary for nodulation and that this dependence improves species development because plant growth increases upon co-inoculation.
[Mh] Termos MeSH primário: Fabaceae/microbiologia
Micorrizas
Bactérias Fixadoras de Nitrogênio
Simbiose
[Mh] Termos MeSH secundário: Fixação de Nitrogênio
Nodulação
Nódulos Radiculares de Plantas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE


  6 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27838542
[Au] Autor:Rodak BW; Freitas DS; Bamberg SM; Carneiro MA; Guilherme LR
[Ad] Endereço:Department of Soil Science, Federal University of Lavras (UFLA), University Campus, 372000-000, Lavras, Minas Gerais, Brazil.
[Ti] Título:X-ray microanalytical studies of mineral elements in the tripartite symbiosis between lima bean, N -fixing bacteria and mycorrhizal fungi.
[So] Source:J Microbiol Methods;132:14-20, 2017 Jan.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The symbiosis between legumes, arbuscular mycorrhizal (AM) fungi, and N -fixing bacteria (NFB) provides mutual nutritional gains. However, assessing the nutritional status of the microorganisms is a difficult task. A methodology that could assess this status, in situ, could assist managing these organisms in agriculture. This study used X-ray microanalyses to quantify and locate mineral elements in structures formed in a tripartite symbiosis. Lima bean (Phaseolus lunatus L. Walp) was cultivated in pots under greenhouse conditions, to which we have added AM fungal isolates (Glomus macrocarpum and Acaulospora colombiana) and NFB (Bradyrhizobium japonicum) inocula. Uninoculated control plants were also included. Symbionts were evaluated at the onset of flowering. Quantification of the mineral elements in the symbiotic components was performed using energy dispersive X-ray spectroscopy (EDX) and a scanning electron microscopy (SEM) was used to identify structures. EDX analysis detected 13 elements with the most abundant being N, Ca, and Se, occurring in all tissues, Fe in roots, Ni and Al in epidermis and P and Mo in nodules. Elemental quantification in fungal structures was not possible. The distribution of elements was related to their symbiotic function. X-ray microanalysis can be efficiently applied for nutritional diagnosis in tripartite symbiosis.
[Mh] Termos MeSH primário: Fabaceae/microbiologia
Micorrizas/isolamento & purificação
Bactérias Fixadoras de Nitrogênio/isolamento & purificação
Simbiose
Oligoelementos/análise
[Mh] Termos MeSH secundário: Microanálise por Sonda Eletrônica
Fabaceae/química
Microscopia Eletrônica de Varredura
Tamanho da Partícula
Raízes de Plantas/química
Raízes de Plantas/microbiologia
Espectrometria por Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Trace Elements)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161114
[St] Status:MEDLINE


  7 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27581036
[Au] Autor:Wolinska A; Kuzniar A; Zielenkiewicz U; Banach A; Izak D; Stepniewska Z; Blaszczyk M
[Ad] Endereço:Department of Biochemistry and Environmental Chemistry, Institute of Biotechnology, The John Paul II Catholic University of Lublin, 1 I Konstantynów Str, 20-708, Lublin, Poland. awolin@kul.pl.
[Ti] Título:Metagenomic Analysis of Some Potential Nitrogen-Fixing Bacteria in Arable Soils at Different Formation Processes.
[So] Source:Microb Ecol;73(1):162-176, 2017 Jan.
[Is] ISSN:1432-184X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The main goal of the study was to determine the diversity of the potential nitrogen-fixing (PNF) bacteria inhabiting agricultural (A) soils versus wastelands serving as controls (C). The soils were classified into three groups based on the formation process: autogenic soils (Albic Luvisols, Brunic Arenosols, Haplic Phaeozem) formed on loess material, hydrogenic soils (Mollic Gleysols, Eutric Fluvisol, Eutric Histosol) formed under the effect of stagnant water and lithogenic soils (Rendzina Leptosols) formed on limestone. In order to determine the preferable conditions for PNF bacteria, the relationships between the soil chemical features and bacterial operational taxonomic units (OTUs) were tested. Additionally, the nitrogen content and fertilisation requirement of the lithogenic (LG), autogenic (AG) and hydrogenic (HG) soils were discussed. The composition of the bacterial communities was analysed with the next-generation sequencing (NGS) by the Ion Torrent™ technology. The sequences were clustered into OTU based on a 99 % similarity threshold. The arable soils tested were distinctly dominated by ß-Proteobacteria representatives of PNF bacteria belonging to the genus Burkholderia. Bacteria from the α-Proteobacteria class and Devosia genus were subdominants. A free-living Cyanobacteria population dominated in A rather than in C soils. We have found that both soil agricultural management and soil formation processes are the most conducive factors for PNF bacteria, as a majority of these microorganisms inhabit the AG group of soils, whilst the LG soils with the lowest abundance of PNF bacteria revealed the need for additional mineral fertilisation. Our studies have also indicated that there are close relationships between soil classification with respect to soil formation processes and PNF bacteria preference for occupation of soil niches.
[Mh] Termos MeSH primário: Cianobactérias/classificação
Cianobactérias/isolamento & purificação
Bactérias Fixadoras de Nitrogênio/classificação
Bactérias Fixadoras de Nitrogênio/metabolismo
Proteobactérias/classificação
Proteobactérias/isolamento & purificação
Microbiologia do Solo
Solo/química
[Mh] Termos MeSH secundário: Agricultura
Biodiversidade
Cianobactérias/genética
Metagenoma/genética
Bactérias Fixadoras de Nitrogênio/genética
Polônia
Proteobactérias/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160902
[St] Status:MEDLINE
[do] DOI:10.1007/s00248-016-0837-2


  8 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27409290
[Au] Autor:Rangel WM; Thijs S; Janssen J; Oliveira Longatti SM; Bonaldi DS; Ribeiro PR; Jambon I; Eevers N; Weyens N; Vangronsveld J; Moreira FM
[Ad] Endereço:a Biology Department , Federal University of Lavras (UFLA) , Lavras , Minas Gerais , Brazil.
[Ti] Título:Native rhizobia from Zn mining soil promote the growth of Leucaena leucocephala on contaminated soil.
[So] Source:Int J Phytoremediation;19(2):142-156, 2017 Feb.
[Is] ISSN:1549-7879
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plants on contaminated mining soils often show a reduced growth due to nutrient depletion as well as trace elements (TEs) toxicity. Since those conditions threat plant's survival, plant growth-promoting rhizobacteria (PGPRs), such as rhizobia, might be of crucial importance for plant colonization on TE-contaminated soils. Native rhizobia from mining soils are promising candidates for bioaugmented phytoremediation of those soils as they are adapted to the specific conditions. In this work, rhizobia from Zn- and Cd-contaminated mining soils were in vitro screened for their PGP features [organic acids, indole-3-acetic acid (IAA), and siderophore (SID) production; 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity; and Ca (PO ) solubilization] and Zn and Cd tolerance. In addition, some type and reference rhizobia strains were included in the study as well. The in vitro screening indicated that rhizobia and other native genera have great potential for phytoremediation purposes, by exerting, besides biological N fixation, other plant growth-promoting traits. Leucaena leucocephala-Mesorhizobium sp. (UFLA 01-765) showed multielement tolerance and an efficient symbiosis on contaminated soil, decreasing the activities of antioxidative enzymes in shoots. This symbiosis is a promising combination for phytostabilization.
[Mh] Termos MeSH primário: Fabaceae/metabolismo
Fabaceae/microbiologia
Bactérias Fixadoras de Nitrogênio/metabolismo
Microbiologia do Solo
Poluentes do Solo/metabolismo
Simbiose
Zinco/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
DNA Bacteriano/genética
Mesorhizobium/classificação
Mesorhizobium/genética
Mesorhizobium/metabolismo
Bactérias Fixadoras de Nitrogênio/classificação
Bactérias Fixadoras de Nitrogênio/genética
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (Soil Pollutants); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160714
[St] Status:MEDLINE
[do] DOI:10.1080/15226514.2016.1207600


  9 / 15 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27318157
[Au] Autor:Sun J; Qian X; Gu J; Wang X; Gao H
[Ad] Endereço:College of Resources and Environmental Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.
[Ti] Título:Effects of oxytetracycline on the abundance and community structure of nitrogen-fixing bacteria during cattle manure composting.
[So] Source:Bioresour Technol;216:801-7, 2016 Sep.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effects of oxytetracycline (OTC) on nitrogen-fixing bacterial communities were investigated during cattle manure composting. The abundance and community structure of nitrogen-fixing bacteria were determined by qPCR and denaturing gradient gel electrophoresis (DGGE), respectively. The matrix was spiked with OTC at four levels: no OTC, 10mg/kg dry weight (DW) OTC (L), 60mg/kg DW OTC (M), and 200mg/kg DW OTC (H). The high temperature period of composting was shorter with M and H, and the decline in temperature during the cooling stage was accelerated by OTC. OTC had a concentration-dependent inhibitory effect on the nitrogenase activity during early composting, and the nifH gene abundance declined significantly during the later composting stage. The DGGE profile and statistical analysis showed that OTC changed the nitrogen-fixing bacterial community succession and reduced the community richness and dominance. The nitrogen-fixing bacterial community structure was affected greatly by the high level of OTC.
[Mh] Termos MeSH primário: Esterco/análise
Bactérias Fixadoras de Nitrogênio/efeitos dos fármacos
Bactérias Fixadoras de Nitrogênio/crescimento & desenvolvimento
Oxitetraciclina/farmacologia
Solo/química
[Mh] Termos MeSH secundário: Animais
Biodiversidade
Bovinos
Eletroforese em Gel de Gradiente Desnaturante
Genes Bacterianos
Bactérias Fixadoras de Nitrogênio/genética
Nitrogenase/genética
Nitrogenase/metabolismo
Filogenia
Análise de Sequência de DNA
Microbiologia do Solo
Temperatura Ambiente
Triticum/química
Resíduos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Manure); 0 (Soil); 0 (Waste Products); EC 1.18.6.1 (Nitrogenase); X20I9EN955 (Oxytetracycline)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160619
[St] Status:MEDLINE


  10 / 15 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27287843
[Au] Autor:Teixeira H; Rodríguez-Echeverría S
[Ad] Endereço:CFE - Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal.
[Ti] Título:Identification of symbiotic nitrogen-fixing bacteria from three African leguminous trees in Gorongosa National Park.
[So] Source:Syst Appl Microbiol;39(5):350-8, 2016 Jul.
[Is] ISSN:1618-0984
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The symbiosis between leguminous plants and symbiotic nitrogen-fixing bacteria is a key component of terrestrial ecosystems. Woody legumes are well represented in tropical African forests but despite their ecological and socio-economic importance, they have been little studied for this symbiosis. In this study, we examined the identity and diversity of symbiotic-nitrogen fixing bacteria associated with Acacia xanthophloea, Faidherbia albida and Albizia versicolor in the Gorongosa National Park (GNP) in Mozambique. To the best of our knowledge, this is the first report on the identity of symbiotic-nitrogen fixing bacteria in this region. 166 isolates were obtained and subjected to molecular identification. BOX-A1R PCR was used to discriminate different bacterial isolates and PCR-sequencing of 16S rDNA, and two housekeeping genes, glnII and recA, was used to identify the obtained bacteria. The gene nifH was also analyzed to assess the symbiotic capacity of the obtained bacteria. All isolates from F. albida and Al. versicolor belonged to the Bradyrhizobium genus whereas isolates from Ac. xanthophloea clustered with Mesorhizobium, Rhizobium or Ensifer strains. Soil chemical analysis revealed significant differences between the soils occupied by the three studied species. Thus, we found a clear delimitation in the rhizobial communities and soils associated with Ac. xanthophloea, F. albida and Al. versicolor, and higher rhizobial diversity for Ac. xanthophloea than previously reported.
[Mh] Termos MeSH primário: Acacia/microbiologia
Albizzia/microbiologia
Bradyrhizobium/classificação
Bradyrhizobium/genética
Mesorhizobium/classificação
Mesorhizobium/genética
Bactérias Fixadoras de Nitrogênio/classificação
Bactérias Fixadoras de Nitrogênio/isolamento & purificação
Rhizobium/classificação
Rhizobium/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Bradyrhizobium/isolamento & purificação
DNA Bacteriano/genética
Florestas
Genes Essenciais/genética
Variação Genética/genética
Mesorhizobium/isolamento & purificação
Tipagem Molecular
Moçambique
Bactérias Fixadoras de Nitrogênio/genética
Oxirredutases/genética
Parques Recreativos
Filogenia
RNA Ribossômico 16S/genética
Recombinases Rec A/genética
Rhizobium/isolamento & purificação
Análise de Sequência de DNA
Solo/química
Microbiologia do Solo
Simbiose
Árvores/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (Soil); EC 1.- (Oxidoreductases); EC 1.18.6.1 (nitrogenase reductase); EC 2.7.7.- (Rec A Recombinases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160612
[St] Status:MEDLINE



página 1 de 2 ir para página        
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde