Base de dados : MEDLINE
Pesquisa : B03.660.050 [Categoria DeCS]
Referências encontradas : 1679 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 168 ir para página                         

  1 / 1679 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29458471
[Au] Autor:Schellenberg J; Busse HJ; Hardt M; Schubert P; Wilke T; Kämpfer P; Glaeser SP
[Ad] Endereço:1​Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
[Ti] Título:Proposal of Litorimonas haliclonae sp. nov., isolated from a marine sponge of the genus Haliclona.
[So] Source:Int J Syst Evol Microbiol;68(3):835-843, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42 , was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany. The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5). Strain MAA42 shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis. Sequence similarities to all other closely related type strains were below 97 %. DNA-DNA hybridization of strain MAA42 with L. taeanensis DSM 22008 resulted in values of 4.7 % (reciprocal 17.7 %). Major cellular fatty acids of strain MAA42 were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %). Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone. The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid. Glucuronopyranosyldiglyceride was present as a minor compound. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The genomic DNA G+C content was 52.8 mol%. Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42 represents a novel species of the genus Litorimonas, for which the name Litorimonas haliclonae is proposed. The type strain is MAA42 (=CCM 8709 =CIP 111178 =LMG 29765 ).
[Mh] Termos MeSH primário: Alphaproteobacteria/classificação
Haliclona/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Alemanha
Hibridização de Ácido Nucleico
Peptidoglicano/química
Fosfolipídeos/química
Pigmentação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Espermidina/química
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); 583-93-7 (Diaminopimelic Acid); I7T5V2W47R (Ubiquinone Q2); U87FK77H25 (Spermidine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002592


  2 / 1679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29368769
[Au] Autor:Malý P; Gardiner AT; Cogdell RJ; van Grondelle R; Mancal T
[Ad] Endereço:Department of Biophysics, Faculty of Sciences, Vrije Universiteit Amsterdam, De Boeleaan 1081, 1081HV Amsterdam, The Netherlands. p.maly@vu.nl.
[Ti] Título:Robust light harvesting by a noisy antenna.
[So] Source:Phys Chem Chem Phys;20(6):4360-4372, 2018 Feb 07.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Photosynthetic light harvesting can be very efficient in solar energy conversion while taking place in a highly disordered and noisy physiological environment. This efficiency is achieved by the ultrafast speed of the primary photosynthetic processes, which is enabled by a delicate interplay of quantum effects, thermodynamics and environmental noise. The primary processes take place in light-harvesting antennas built from pigments bound to a fluctuating protein scaffold. Here, we employ ultrafast single-molecule spectroscopy to follow fluctuations of the femtosecond energy transfer times in individual LH2 antenna complexes of purple bacteria. By combining single molecule results with ensemble spectroscopy through a unified theoretical description of both, we show how the protein fluctuations alter the excitation energy transfer dynamics. We find that from the thirteen orders of magnitude of possible timescales from picoseconds to minutes, the relevant fluctuations occur predominantly on a biological timescale of seconds, i.e. in the domain of slow protein motion. The measured spectra and dynamics can be explained by the protein modulating pigment excitation energies only. Moreover, we find that the small spread of pigment mean energies allows for excitation delocalization between the coupled pigments to survive. These unique features provide fast energy transport even in the presence of disorder. We conclude that this is the mechanism that enables LH2 to operate as a robust light-harvester, in spite of its intrinsically noisy biological environment.
[Mh] Termos MeSH primário: Complexos de Proteínas Captadores de Luz/química
[Mh] Termos MeSH secundário: Alphaproteobacteria/metabolismo
Transferência de Energia
Complexos de Proteínas Captadores de Luz/metabolismo
Estrutura Quaternária de Proteína
Teoria Quântica
Espectrometria de Fluorescência
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Light-Harvesting Protein Complexes)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp06139k


  3 / 1679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29287245
[Au] Autor:Sabaneyeva E; Castelli M; Szokoli F; Benken K; Lebedeva N; Salvetti A; Schweikert M; Fokin S; Petroni G
[Ad] Endereço:Department of Cytology and Histology, St. Petersburg State University, Russian Federation. Electronic address: e.sabaneeva@spbu.ru.
[Ti] Título:Host and symbiont intraspecific variability: The case of Paramecium calkinsi and "Candidatus Trichorickettsia mobilis".
[So] Source:Eur J Protistol;62:79-94, 2018 Feb.
[Is] ISSN:1618-0429
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Newly isolated strains of the ciliate Paramecium calkinsi and their cytoplasmic bacterial endosymbionts were characterized by a multidisciplinary approach, including live observation, ultrastructural investigation, and molecular analysis. Despite morphological resemblance, the characterized P. calkinsi strains showed a significant molecular divergence compared to conspecifics, possibly hinting for a cryptic speciation. The endosymbionts were clearly found to be affiliated to the species "Candidatus Trichorickettsia mobilis" (Rickettsiales, Rickettsiaceae), currently encompassing only bacteria retrieved in an obligate intracellular association with other ciliates. However, a relatively high degree of intraspecific divergence was observed as well, thus it was possible to split "Candidatus Trichorickettsia" into three subspecies, one of which represented so far only by the newly characterized endosymbionts of P. calkinsi. Other features distinguished the members of each different subspecies. In particular, the endosymbionts of P. calkinsi resided in the cytoplasm and possessed numerous peritrichous flagella, although no motility was evidenced, whereas their conspecifics in other hosts were either cytoplasmic and devoid of flagella, or macronuclear, displaying flagellar-driven motility. Moreover, contrarily to previously analyzed "Candidatus Trichorickettsia" hosts, infected P. calkinsi cells frequently became amicronucleate and demonstrated abnormal cell division, eventually leading to decline of the laboratory culture.
[Mh] Termos MeSH primário: Alphaproteobacteria/fisiologia
Interações Hospedeiro-Parasita
Paramecium/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE


  4 / 1679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29265998
[Au] Autor:Li Y; Xu G; Lin C; Wang X; Piao CG
[Ad] Endereço:The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
[Ti] Título:Aureimonas populi sp. nov., isolated from poplar tree bark.
[So] Source:Int J Syst Evol Microbiol;68(2):487-491, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two novel bacterial strains (4M3-2 and 10-107-7) were isolated from poplar tree bark. The strains were Gram-stain-negative facultative aerobes, and produced short rods that were motile because of polar flagella. A phylogenetic tree was reconstructed based on 16S rRNA gene sequences indicating that the two novel strains are related to species of the genus Aureimonas and Aurantimonas. The two novel strains shared the highest 16S rRNA gene sequence similarities with Aureimonasfrigidaquae CW5 7Y-4 (97.1 %) and Aureimonasaltamirensis DSM 21988 (96.6 %)o. The lipids of the novel strain contain diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine and sulfoquinovosyl diacylglycerol. The presence of a distinct glycolipid (sulfoquinovosyl diacylglycerol) is an important chemotaxonomic feature used to distinguish between species of the genera, Aurantimonas and Aureimonas. Additionally, the DNA-DNA hybridization results indicated that the two novel strains represent a novel taxon distinct from Aureimonas frigidaquae. The results of the 16S rRNA gene sequence analysis, as well as the physiological and biochemical characteristics imply that the two novel strains should be assigned to a novel species, with the proposed name Aureimonas populi sp. nov. The type strain is 4M3-2 (=CFCC 11187 =KCTC 42087 ).
[Mh] Termos MeSH primário: Alphaproteobacteria/classificação
Filogenia
Casca de Planta/microbiologia
Populus/microbiologia
[Mh] Termos MeSH secundário: Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Glicolipídeos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Glycolipids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002479


  5 / 1679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29364604
[Au] Autor:Galach'yants AD; Bel'kova NL; Sukhanova EV; Romanovskaya VA; Gladka GV; Bedoshvili ED; Parfenova VV
[Ti] Título:[Diversity and Physiological and Biochemical Properties of Heterotrophic Bacteria. Isolated from Lake Baikal Neuston.]
[So] Source:Mikrobiologiia;85(5):568-579, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:For heterotrophic microorganisms (44 strains) isolated-from the surface film of Lake Baikal, iden- tification was carried out and their. physiological and biochemical characteristics were determined. Com- pared to the water column, diversity of cultured heterotrophs was low, indicating formation of stable micro- bial communities at the air-water interphase interface. Heterotrophic bacteria isolated from the surface mi- crolayer exhibited the enzymatic activity comparable to that for strains form other biofilm associations. Deinococcusfi6us strain NA202 'vas the most active component of the community, capable of utilization of the broadest spectrum of mono- and disaccharides,'sugars, and amino acids. This strain possessed the highest diversity of extracellular enzymes and was the most resistant to UV radiation. The physiological and bio- chemical properties of this strain may-be responsible for its adaptation to survival in extreme conditions of the surface microlayer. Our results improve our understanding of occurrence of UV-resistant strains in freshwater ecosystems.
[Mh] Termos MeSH primário: Deinococcus/metabolismo
Processos Heterotróficos/fisiologia
Lagos/microbiologia
Consórcios Microbianos/fisiologia
Filogenia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Alphaproteobacteria/classificação
Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Alphaproteobacteria/efeitos da radiação
Aminoácidos/metabolismo
Bacteroidetes/classificação
Bacteroidetes/genética
Bacteroidetes/isolamento & purificação
Bacteroidetes/efeitos da radiação
Betaproteobacteria/classificação
Betaproteobacteria/genética
Betaproteobacteria/isolamento & purificação
Betaproteobacteria/efeitos da radiação
Biodiversidade
Deinococcus/classificação
Deinococcus/isolamento & purificação
Deinococcus/efeitos da radiação
Deltaproteobacteria/classificação
Deltaproteobacteria/genética
Deltaproteobacteria/isolamento & purificação
Deltaproteobacteria/efeitos da radiação
Dissacarídeos/metabolismo
Ecossistema
Firmicutes/classificação
Firmicutes/genética
Firmicutes/isolamento & purificação
Firmicutes/efeitos da radiação
Gammaproteobacteria/classificação
Gammaproteobacteria/genética
Gammaproteobacteria/isolamento & purificação
Gammaproteobacteria/efeitos da radiação
Processos Heterotróficos/efeitos da radiação
Consórcios Microbianos/efeitos da radiação
Monossacarídeos/metabolismo
Proteobactérias/classificação
Proteobactérias/genética
Proteobactérias/isolamento & purificação
Proteobactérias/efeitos da radiação
Sibéria
Propriedades de Superfície
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Disaccharides); 0 (Monosaccharides); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


  6 / 1679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29364602
[Au] Autor:Danilova OV; Belova SE; Gagarinova IV; Dedysh SN
[Ti] Título:Microbial Community Composition and Methanotroph Diversity of a Subarctic Wetland in Russia.
[So] Source:Mikrobiologiia;85(5):545-554, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:This study assessed the microbial diversity, activity, and composition of methane-oxidizing communities of a subarctic wetland in Russia,with mosaic cover of Sphagnum mosses and lichens of the genera Cladonia and Cetraria. Potential methane-oxidizing activity of peat sampled from lichen-dominated wetland sites was higher than that in the sites dominated by Sphagnum mosses. In peat from lichendominated sites, major bacterial groups identified by high-throughput sequencing of the 16S rRNA genes were the Acidobacteria (35.4-41.2% of total 16S rRNA gene reads), Alphaproteobacteria (19.1-24.2%), Gammaproteobacteria (7.9-11.1%), Actinobacteria (5.5-13.2%), Planctomycetes (7.2-9.5%), and Verrucomicrobia (5.1-9.5%). The distinctive feature of this community was high proportion of Subdivision 2 Acidobacteria, which are not char- acteristic for boreal Sphagnum peat bogs. Methanotrophic community composition was determined by mo- lecular analysis of the pmoA gene encoding particulate methane monooxygenase. Most (-80%) of all pmoA gene fragments revealed in peat from lichen-dominated sites belonged to the phylogenetic lineage represented by a microaerobic spiral-shaped methanotroph, "Candidatus Methylospira mobilis." Members of the genus Methylocystis, which are typical inhabitants of boreal Sphagnum peat bogs, represented only a minor group of indigenous methanotrophs. The specific feature of a methanotrophic community in peat from lichen-dominated sites was the presence of uncultivated USCa (Upland Soil Cluster alpha) methanotrophs, which are typical for acidic upland soils showing atmospheric methane oxidation. The methanotrophic community composition in lichen-dominated sites of a tundra wetland, therefore, was markedly different from that in bo- real Sphagnum peat bogs.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Água Subterrânea/microbiologia
Metano/metabolismo
Consórcios Microbianos/fisiologia
Oxigenases/genética
[Mh] Termos MeSH secundário: Acidobacteria/classificação
Acidobacteria/genética
Acidobacteria/isolamento & purificação
Acidobacteria/metabolismo
Actinobacteria/classificação
Actinobacteria/genética
Actinobacteria/isolamento & purificação
Actinobacteria/metabolismo
Alphaproteobacteria/classificação
Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Alphaproteobacteria/metabolismo
Regiões Árticas
Proteínas de Bactérias/metabolismo
Briófitas/fisiologia
Gammaproteobacteria/classificação
Gammaproteobacteria/genética
Gammaproteobacteria/isolamento & purificação
Gammaproteobacteria/metabolismo
Expressão Gênica
Líquens/fisiologia
Metano/química
Oxigenases/metabolismo
Filogenia
Planctomycetales/classificação
Planctomycetales/genética
Planctomycetales/isolamento & purificação
Planctomycetales/metabolismo
Federação Russa
Verrucomicrobia/classificação
Verrucomicrobia/genética
Verrucomicrobia/isolamento & purificação
Verrucomicrobia/metabolismo
Zonas Úmidas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 1.13.- (Oxygenases); EC 1.14.13.25 (methane monooxygenase); OP0UW79H66 (Methane)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


  7 / 1679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28460054
[Au] Autor:Zhou N; Zhao S; Tian CY
[Ad] Endereço:State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.
[Ti] Título:Effect of halotolerant rhizobacteria isolated from halophytes on the growth of sugar beet (Beta vulgaris L.) under salt stress.
[So] Source:FEMS Microbiol Lett;364(11), 2017 Jun 15.
[Is] ISSN:1574-6968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Utilization of rhizobacteria that have associated with plant roots in harsh environments could be a feasible strategy to deal with limits to agricultural production caused by soil salinity. Halophytes occur naturally in high-salt environments, and their roots may be associated with promising microbial candidates for promoting growth and salt tolerance in crops. This study aimed to isolate efficient halotolerant plant-growth-promoting rhizobacterial strains from halophytes and evaluate their activity and effects on sugar beet (Beta vulgaris L.) growth under salinity stress. A total of 23 isolates were initially screened for their ability to secrete 1-aminocyclopropane-1-carboxylate deaminase (ACD) as well as other plant-growth-promoting characteristics and subsequently identified by sequencing the 16S rRNA gene. Three isolates, identified as Micrococcus yunnanensis, Planococcus rifietoensis and Variovorax paradoxus, enhanced salt stress tolerance remarkably in sugar beet, resulting in greater seed germination and plant biomass, higher photosynthetic capacity and lower stress-induced ethylene production at different NaCl concentrations (50-125 mM). These results demonstrate that salinity-adapted, ACD-producing bacteria isolated from halophytes could promote sugar beet growth under saline stress conditions.
[Mh] Termos MeSH primário: Alphaproteobacteria/classificação
Beta vulgaris/microbiologia
Raízes de Plantas/microbiologia
Plantas Tolerantes a Sal/microbiologia
Estresse Fisiológico
[Mh] Termos MeSH secundário: Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Beta vulgaris/crescimento & desenvolvimento
Biomassa
Carbono-Carbono Liases/metabolismo
Etilenos/metabolismo
Micrococcus/isolamento & purificação
Micrococcus/metabolismo
Bactérias Planococcus/isolamento & purificação
Bactérias Planococcus/metabolismo
RNA Ribossômico 16S/genética
Salinidade
Solo/química
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenes); 0 (RNA, Ribosomal, 16S); 0 (Soil); 91GW059KN7 (ethylene); EC 3.5.99.7 (1-aminocyclopropane-1-carboxylate deaminase); EC 4.1.- (Carbon-Carbon Lyases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1093/femsle/fnx091


  8 / 1679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29223156
[Au] Autor:But SY; Egorova SV; Khmelenina VN; Trotsenko YA
[Ad] Endereço:Skryabin Institute of Biochemistry and Physiology of Microorganisms, Laboratory of Methylotrophy, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. flash20063@rambler.ru.
[Ti] Título:Biochemical Properties and Phylogeny of Hydroxypyruvate Reductases from Methanotrophic Bacteria with Different C -Assimilation Pathways.
[So] Source:Biochemistry (Mosc);82(11):1295-1303, 2017 Nov.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the aerobic methanotrophic bacteria Methylomicrobium alcaliphilum 20Z, Methylococcus capsulatus Bath, and Methylosinus trichosporium OB3b, the biochemical properties of hydroxypyruvate reductase (Hpr), an indicator enzyme of the serine pathway for assimilation of reduced C -compounds, were comparatively analyzed. The recombinant Hpr obtained by cloning and heterologous expression of the hpr gene in Escherichia coli catalyzed NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but did not catalyze the reverse reactions of D-glycerate or glycolate oxidation. The absence of the glycerate dehydrogenase activity in the methanotrophic Hpr confirmed a key role of the enzyme in utilization of C -compounds via the serine cycle. The enzyme from Ms. trichosporium OB3b realizing the serine cycle as a sole assimilation pathway had much higher special activity and affinity in comparison to Hpr from Mm. alcaliphilum 20Z and Mc. capsulatus Bath assimilating carbon predominantly via the ribulose monophosphate (RuMP) cycle. The hpr gene was found as part of gene clusters coding the serine cycle enzymes in all sequenced methanotrophic genomes except the representatives of the Verrucomicrobia phylum. Phylogenetic analyses revealed two types of Hpr: (i) Hpr of methanotrophs belonging to the Gammaproteobacteria class, which use the serine cycle along with the RuMP cycle, as well as of non-methylotrophic bacteria belonging to the Alphaproteobacteria class; (ii) Hpr of methylotrophs from Alpha- and Betaproteobacteria classes that use only the serine cycle and of non-methylotrophic representatives of Betaproteobacteria. The putative role and origin of hydroxypyruvate reductase in methanotrophs are discussed.
[Mh] Termos MeSH primário: Hidroxipiruvato Redutase/classificação
Methylococcaceae/enzimologia
Methylosinus/enzimologia
Filogenia
[Mh] Termos MeSH secundário: Alphaproteobacteria
Gammaproteobacteria
Bactérias Aeróbias Gram-Negativas/classificação
Bactérias Aeróbias Gram-Negativas/enzimologia
Hidroxipiruvato Redutase/metabolismo
Methylobacillus
Methylobacteriaceae
Methylophilaceae
Serina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
452VLY9402 (Serine); EC 1.1.1.81 (Hydroxypyruvate Reductase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917110074


  9 / 1679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29240843
[Au] Autor:Dan H; Ikeda N; Fujikami M; Nakabachi A
[Ad] Endereço:Department of Environmental and Life Sciences, Toyohashi University of Technology, Toyohashi, Aichi, Japan.
[Ti] Título:Behavior of bacteriome symbionts during transovarial transmission and development of the Asian citrus psyllid.
[So] Source:PLoS One;12(12):e0189779, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae) is a serious pest worldwide, transmitting Candidatus Liberibacter spp. (Alphaproteobacteria), the causative agents of a devastating citrus disease known as huanglongbing or greening disease. In a symbiotic organ called the bacteriome, D. citri possesses an organelle-like defensive symbiont, Candidatus Profftella armatura (Betaproteobacteria), and a nutritional symbiont, Ca. Carsonella ruddii (Gammaproteobacteria). Drastically reduced symbiont genomes and metabolic complementarity among the symbionts and D. citri indicate their mutually indispensable association. Moreover, horizontal gene transfer between the Profftella and Liberibacter lineages suggests ecological and evolutionary interactions between the bacteriome symbiont and the HLB pathogen. Using fluorescence in situ hybridization, we examined the behavior of Profftella and Carsonella during transovarial transmission and the development of D. citri. In the bacteriomes of sexually-mature female adults, symbionts transformed from an extremely elongated tubular form into spherical or short-rod forms, which migrated toward the ovary. The symbionts then formed mosaic masses, which entered at the posterior pole of the vitellogenic oocytes. After anatrepsis, Carsonella and Profftella migrated to the central and peripheral parts of the mass, respectively. Following the appearance of host nuclei, the mass cellularized, segregating Carsonella and Profftella in the central syncytium and peripheral uninucleate bacteriocytes, respectively. Subsequently, the uninucleate bacteriocytes harboring Profftella assembled at the posterior pole, while the syncytium, containing Carsonella, sat on the anterior side facing the germ band initiating katatrepsis. During dorsal closure, the syncytium was divided into uninuclear bacteriocytes, which surrounded the mass of bacteriocytes containing Profftella. Once fully surrounded, the bacteriocyte mass containing Profftella was fused into a syncytium. Prior to hatching, a pair of wing-like protrusions arose from both lateral sides of the bacteriome, which continued to grow throughout the nymphal stages. These findings provide a foundation for better understanding the intricate relationship between D. citri and its microbiota.
[Mh] Termos MeSH primário: Alphaproteobacteria/patogenicidade
Infecções Bacterianas/microbiologia
Citrus/parasitologia
Hemípteros/microbiologia
Insetos Vetores
Ovário/microbiologia
Doenças das Plantas/microbiologia
[Mh] Termos MeSH secundário: Animais
Feminino
Hemípteros/fisiologia
Interações Hospedeiro-Parasita
Interações Hospedeiro-Patógeno
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189779


  10 / 1679 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28931093
[Au] Autor:Smith JA; Bar-Peled M
[Ad] Endereço:Complex Carbohydrate Research Center (CCRC), University of Georgia, Athens, GA, United States of America.
[Ti] Título:Synthesis of UDP-apiose in Bacteria: The marine phototroph Geminicoccus roseus and the plant pathogen Xanthomonas pisi.
[So] Source:PLoS One;12(9):e0184953, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The branched-chain sugar apiose was widely assumed to be synthesized only by plant species. In plants, apiose-containing polysaccharides are found in vascularized plant cell walls as the pectic polymers rhamnogalacturonan II and apiogalacturonan. Apiosylated secondary metabolites are also common in many plant species including ancestral avascular bryophytes and green algae. Apiosyl-residues have not been documented in bacteria. In a screen for new bacterial glycan structures, we detected small amounts of apiose in methanolic extracts of the aerobic phototroph Geminicoccus roseus and the pathogenic soil-dwelling bacteria Xanthomonas pisi. Apiose was also present in the cell pellet of X. pisi. Examination of these bacterial genomes uncovered genes with relatively low protein homology to plant UDP-apiose/UDP-xylose synthase (UAS). Phylogenetic analysis revealed that these bacterial UAS-like homologs belong in a clade distinct to UAS and separated from other nucleotide sugar biosynthetic enzymes. Recombinant expression of three bacterial UAS-like proteins demonstrates that they actively convert UDP-glucuronic acid to UDP-apiose and UDP-xylose. Both UDP-apiose and UDP-xylose were detectable in cell cultures of G. roseus and X. pisi. We could not, however, definitively identify the apiosides made by these bacteria, but the detection of apiosides coupled with the in vivo transcription of bUAS and production of UDP-apiose clearly demonstrate that these microbes have evolved the ability to incorporate apiose into glycans during their lifecycles. While this is the first report to describe enzymes for the formation of activated apiose in bacteria, the advantage of synthesizing apiose-containing glycans in bacteria remains unknown. The characteristics of bUAS and its products are discussed.
[Mh] Termos MeSH primário: Alphaproteobacteria/metabolismo
Carboxiliases/metabolismo
Ervilhas/microbiologia
Açúcares de Uridina Difosfato/biossíntese
Xanthomonas/metabolismo
[Mh] Termos MeSH secundário: Alphaproteobacteria/crescimento & desenvolvimento
Filogenia
Xanthomonas/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (UDP-apiose); 0 (Uridine Diphosphate Sugars); EC 4.1.1.- (Carboxy-Lyases); EC 4.1.1.35 (UDPglucuronate decarboxylase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184953



página 1 de 168 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde