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  1 / 124 MEDLINE  
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[PMID]:28820127
[Au] Autor:Xi J; Wang Y; Yang X; Tao Y; Shao Y; Feng F
[Ad] Endereço:Institute for Applied and Environmental Microbiology, College of Life Science, Inner Mongolia Agricultural University, Huhhot 010018, PR China.
[Ti] Título:Mongoliimonas terrestris gen. nov., sp. nov., isolated from desert soil.
[So] Source:Int J Syst Evol Microbiol;67(8):3010-3014, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, non-motile, aerobic, non-spore-forming, spherical bacterium (strain MIMtkB18T) was isolated from desert soil collected from part of a Mongolian Plateau, territory of Inner Mongolia, PR China. Cell growth could be observed at 20-45 °C (optimum at 40 °C), at a pH of 6-9 (optimum at pH 8.6) and in the presence of 0-1 % (w/v) NaCl (optimum 0 %). The genomic DNA G+C content was 69.6 mol%. 16S rRNA gene sequence analysis showed that strain MIMtkB18T was most closely related to Methylobrevis pamukkalensis PK2T (94.1 %), species of the genus Pleomorphomonas(93.4-94.0 %), and Hartmannibacter diazotrophicus E19T (93.9 %). The sole respiratory quinone was Q-10. The major fatty acids (>5 %) were C18 : 0 (5.7 %) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) (81.6 %). Polar lipids were mainly composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine and unidentified phospholipids. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, it is concluded that strain MIMtkB18T represents a novel genus and species, for which the name Mongoliimonas terrestris sp. nov. is proposed. The type strain is MIMtkB18T (=KCTC 42635T=MCCC 1K00571T).
[Mh] Termos MeSH primário: Clima Desértico
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Methylocystaceae/genética
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002067


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[PMID]:27902265
[Au] Autor:Lv H; Masuda S; Fujitani Y; Sahin N; Tani A
[Ad] Endereço:1​Institute of Plant Science and Resources, Okayama University, Okayama, Japan.
[Ti] Título:Oharaeibacter diazotrophicus gen. nov., sp. nov., a diazotrophic and facultatively methylotrophic bacterium, isolated from rice rhizosphere.
[So] Source:Int J Syst Evol Microbiol;67(3):576-582, 2017 Apr.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel facultatively methanol-utilizing bacterial strain, SM30T, was isolated from rice rhizosphere. Strain SM30T was Gram-stain-negative, aerobic, motile, short rods, and grew optimally at pH 7 and at 28 °C. It could tolerate 0 to 2 % (w/v) NaCl. Based on 16S rRNA gene sequence comparisons, strain SM30T was most closely related to Pleomorphomonas oryzae DSM 16300T, with a low similarity of 94.17 %. One of the lanthanide metals, lanthanum, could enhance its growth slightly on methanol. Phylogenetic trees, based on the mxaF, xoxF and cpn60 genes of SM30T showed its distinct phylogenetic position with respect to species with validly published names. Polymerase chain reaction (PCR) amplification of the nifH and growth on nitrogen-free medium indicated that strain SM30T is a diazotroph. The major cellular fatty acids were summed feature 8 (containing 18 : 1ω7c and 18 : 1ω6c) and cyclo 19 : 0ω8c. The major quinone was ubiquinone 10. The DNA G+C content was 74.6 mol%. Based on the genotypic and phenotypic characteristics, strain SM30T represents a novel genus and species, for which the name Oharaeibacter diazotrophicus gen. nov., sp. nov. is proposed with the type strain SM30T (=NBRC 111955T=DSM 102969T).
[Mh] Termos MeSH primário: Methylocystaceae/classificação
Oryza/microbiologia
Filogenia
Rizosfera
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Japão
Methylocystaceae/genética
Methylocystaceae/isolamento & purificação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001660


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[PMID]:27878346
[Au] Autor:Deng Y; Liu Y; Dumont M; Conrad R
[Ad] Endereço:College of Geographic Sciences, Nanjing Normal University, 1 Wenyuan Road, 210023, Nanjing, China.
[Ti] Título:Salinity Affects the Composition of the Aerobic Methanotroph Community in Alkaline Lake Sediments from the Tibetan Plateau.
[So] Source:Microb Ecol;73(1):101-110, 2017 Jan.
[Is] ISSN:1432-184X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lakes are widely distributed on the Tibetan Plateau, which plays an important role in natural methane emission. Aerobic methanotrophs in lake sediments reduce the amount of methane released into the atmosphere. However, no study to date has analyzed the methanotroph community composition and their driving factors in sediments of these high-altitude lakes (>4000 m). To provide new insights on this aspect, the abundance and composition in the sediments of six high-altitude alkaline lakes (including both freshwater and saline lakes) on the Tibetan Plateau were studied. The quantitative PCR, terminal restriction fragment length polymorphism, and 454-pyrosequencing methods were used to target the pmoA genes. The pmoA gene copies ranged 10 -10 per gram fresh sediment. Type I methanotrophs predominated in Tibetan lake sediments, with Methylobacter and uncultivated type Ib methanotrophs being dominant in freshwater lakes and Methylomicrobium in saline lakes. Combining the pmoA-pyrosequencing data from Tibetan lakes with other published pmoA-sequencing data from lake sediments of other regions, a significant salinity and alkalinity effect (P = 0.001) was detected, especially salinity, which explained ∼25% of methanotroph community variability. The main effect was Methylomicrobium being dominant (up to 100%) in saline lakes only. In freshwater lakes, however, methanotroph composition was relatively diverse, including Methylobacter, Methylocystis, and uncultured type Ib clusters. This study provides the first methanotroph data for high-altitude lake sediments (>4000 m) and shows that salinity is a driving factor for the community composition of aerobic methanotrophs.
[Mh] Termos MeSH primário: Sedimentos Geológicos/química
Sedimentos Geológicos/microbiologia
Lagos/química
Lagos/microbiologia
Metano/metabolismo
Salinidade
Cloreto de Sódio/metabolismo
[Mh] Termos MeSH secundário: Biodiversidade
DNA Bacteriano/genética
Methylobacterium/classificação
Methylobacterium/genética
Methylobacterium/isolamento & purificação
Methylococcaceae/classificação
Methylococcaceae/genética
Methylococcaceae/isolamento & purificação
Methylocystaceae/classificação
Methylocystaceae/genética
Methylocystaceae/isolamento & purificação
Oxirredutases/genética
Filogenia
Polimorfismo de Fragmento de Restrição
Análise de Sequência de DNA
Tibet
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 451W47IQ8X (Sodium Chloride); EC 1.- (Oxidoreductases); OP0UW79H66 (Methane)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE
[do] DOI:10.1007/s00248-016-0879-5


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[PMID]:27646453
[Au] Autor:Zhang Y; Chen JX; Wen LL; Tang Y; Zhao HP
[Ad] Endereço:Department of Environmental Engineering, College of Environmental and Resource Science, Zhejiang University, Hangzhou, China.
[Ti] Título:Effects of salinity on simultaneous reduction of perchlorate and nitrate in a methane-based membrane biofilm reactor.
[So] Source:Environ Sci Pollut Res Int;23(23):24248-24255, 2016 Dec.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This study builds upon prior work showing that methane (CH ) could be utilized as the sole electron donor and carbon source in a membrane biofilm reactor (MBfR) for complete perchlorate (ClO ) and nitrate (NO ) removal. Here, we further investigated the effects of salinity on the simultaneous removal of the two contaminants in the reactor. By testing ClO and NO at different salinities, we found that the reactor performance was very sensitive to salinity. While 0.2 % salinity did not significantly affect the hydrogen-based MBfR for ClO and NO removals, 1 % salinity completely inhibited ClO reduction and significantly lowered NO reduction in the CH -based MBfR. In salinity-free conditions, NO and ClO removal fluxes were 0.171 g N/m -day and 0.091 g/m -day, respectively, but NO removal fluxes dropped to 0.0085 g N/m -day and ClO reduction was completely inhibited when the medium changed to 1 % salinity. Scanning electron microscopy (SEM) showed that the salinity dramatically changed the microbial morphology, which led to the development of wire-like cell structures. Quantitative real-time PCR (qPCR) indicated that the total number of microorganisms and abundances of functional genes significantly declined in the presence of NaCl. The relative abundances of Methylomonas (methanogens) decreased from 31.3 to 5.9 % and Denitratisoma (denitrifiers) decreased from 10.6 to 4.4 % when 1 % salinity was introduced.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Reatores Biológicos/microbiologia
Metano/metabolismo
Nitratos/análise
Percloratos/análise
Poluentes Químicos da Água/análise
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Membranas Artificiais
Mathanococcus/crescimento & desenvolvimento
Mathanococcus/metabolismo
Methylocystaceae/crescimento & desenvolvimento
Methylocystaceae/metabolismo
Methylomonas/crescimento & desenvolvimento
Methylomonas/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Salinidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membranes, Artificial); 0 (Nitrates); 0 (Perchlorates); 0 (Water Pollutants, Chemical); OP0UW79H66 (Methane); VLA4NZX2P4 (perchlorate)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE


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[PMID]:27369086
[Au] Autor:Deng Y; Cui X; Dumont MG
[Ad] Endereço:School of Geography Science Nanjing Normal University, Nanjing 210023, China Department of Biogeochemistry, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch Straße 10, 35043 Marburg, Germany dengyongcui@njnu.edu.cn.
[Ti] Título:Identification of active aerobic methanotrophs in plateau wetlands using DNA stable isotope probing.
[So] Source:FEMS Microbiol Lett;363(16), 2016 Aug.
[Is] ISSN:1574-6968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sedge-dominated wetlands on the Qinghai-Tibetan Plateau are methane emission centers. Methanotrophs at these sites play a role in reducing methane emissions, but relatively little is known about the composition of active methanotrophs in these wetlands. Here, we used DNA stable isotope probing to identify the key active aerobic methanotrophs in three sedge-dominated wetlands on the plateau. We found that Methylocystis species were active in two peatlands, Hongyuan and Dangxiong. Methylobacter species were found to be active only in Dangxiong peat. Hongyuan peat had the highest methane oxidation rate, and cross-feeding of carbon from methanotrophs to methylotrophic Hyphomicrobium species was observed. Owing to a low methane oxidation rate during the incubation, the labeling of methanotrophs in Maduo wetland samples was not detected. Our results indicate that there are large differences in the activity of methanotrophs in the wetlands of this region.
[Mh] Termos MeSH primário: DNA Bacteriano/química
Metano/metabolismo
Methylobacteriaceae/classificação
Methylobacteriaceae/genética
Microbiologia do Solo
Zonas Úmidas
[Mh] Termos MeSH secundário: Aerobiose
Marcação por Isótopo
Methylobacteriaceae/isolamento & purificação
Methylobacteriaceae/metabolismo
Methylocystaceae/genética
Methylocystaceae/isolamento & purificação
Methylocystaceae/metabolismo
Filogenia
RNA Ribossômico 16S
Análise de Sequência de DNA
Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (Soil); OP0UW79H66 (Methane)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160703
[St] Status:MEDLINE


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[PMID]:27046027
[Au] Autor:Yang LQ; Liu L; Salam N; Xiao M; Kim CJ; Hozzein WN; Park DJ; Li WJ; Zhang HW
[Ad] Endereço:1​ State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, PR China 2​ Research Center for Marine Biology and Carbon Sequestration, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101 Qingdao,
[Ti] Título:Chenggangzhangella methanolivorans gen. nov., sp. nov., a member of the family Methylocystaceae, transfer of Methylopila helvetica Doronina et al. 2000 to Albibacter helveticus comb. nov. and emended description of the genus Albibacter.
[So] Source:Int J Syst Evol Microbiol;66(8):2825-30, 2016 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, rod-shaped, non-motile and aerobic bacterial strain, designated CHL1T, was isolated from a sludge sample collected from a sewage treatment tank of an agricultural chemical factory. The strain grew at salinities of 0.5-5 % (w/v) NaCl (optimum 2.5 %). Growth occurred at pH 6.0-8.0 (optimum pH 7.0) and 5-40 °C (optimum 28-30 °C). The genomic DNA G+C content was determined to be 70.4 mol%. Q-10 was detected as the respiratory quinone. The major fatty acids (>10 %) were C18 : 1ω7c and/or C18 : 1ω6c and C16 : 0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified phospholipids and two unidentified aminophospholipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CHL1T formed a distinct clade with Albibacter methylovorans DSM 22840T and Methylopila helvetica DM9T within the family Methylocystaceae. On the basis of phenotypic, chemotaxonomic and phylogenetic characteristics, the strain merits recognition as a representative of a novel species of a new genus within the family Methylocystaceae, for which the name Chenggangzhangella methanolivorans gen. nov., sp. nov. is proposed. The type strain of the type species is CHL1T (=KCTC 42661T=CCTCC AB 2015175T). In addition, the species Methylopila helveticaDoronina et al. (2000) is proposed to be transferred to the genus Albibacter as Albibacterhelveticus comb. nov. (type strain DM9T=CIP 106788=VKM B-2189) on the basis of the phylogenetic analysis. An emended description of the genus Albibacter is also provided.
[Mh] Termos MeSH primário: Methylocystaceae/classificação
Filogenia
Esgotos/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Methylocystaceae/genética
Methylocystaceae/isolamento & purificação
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/análogos & derivados
Ubiquinona/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 0 (Sewage); 1339-63-5 (Ubiquinone); EJ27X76M46 (coenzyme Q10)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160406
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001062


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[PMID]:27033202
[Au] Autor:Lee SJ
[Ad] Endereço:Department of Chemistry and Research Institute of Physics and Chemistry, Chonbuk National University, Jeonju, 54896, Republic of Korea. slee026@jbnu.ac.kr.
[Ti] Título:Hydroxylation of methane through component interactions in soluble methane monooxygenases.
[So] Source:J Microbiol;54(4):277-82, 2016 Apr.
[Is] ISSN:1976-3794
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Methane hydroxylation through methane monooxygenases (MMOs) is a key aspect due to their control of the carbon cycle in the ecology system and recent applications of methane gas in the field of bioenergy and bioremediation. Methanotropic bacteria perform a specific microbial conversion from methane, one of the most stable carbon compounds, to methanol through elaborate mechanisms. MMOs express particulate methane monooxygenase (pMMO) in most strains and soluble methane monooxygenase (sMMO) under copper-limited conditions. The mechanisms of MMO have been widely studied from sMMO belonging to the bacterial multicomponent monooxygenase (BMM) superfamily. This enzyme has diiron active sites where different types of hydrocarbons are oxidized through orchestrated hydroxylase, regulatory and reductase components for precise control of hydrocarbons, oxygen, protons, and electrons. Recent advances in biophysical studies, including structural and enzymatic achievements for sMMO, have explained component interactions, substrate pathways, and intermediates of sMMO. In this account, oxidation of methane in sMMO is discussed with recent progress that is critical for understanding the microbial applications of C-H activation in one-carbon substrates.
[Mh] Termos MeSH primário: Metano/metabolismo
Methylococcaceae/metabolismo
Methylocystaceae/metabolismo
Oxigenases/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Domínio Catalítico
Hidroxilação
Methylococcaceae/enzimologia
Methylocystaceae/enzimologia
Complexos Multienzimáticos
Oxirredução
Oxigenases/química
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Multienzyme Complexes); EC 1.13.- (Oxygenases); EC 1.14.13.25 (methane monooxygenase); OP0UW79H66 (Methane)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE
[do] DOI:10.1007/s12275-016-5642-6


  8 / 124 MEDLINE  
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[PMID]:26920242
[Au] Autor:Cal AJ; Sikkema WD; Ponce MI; Franqui-Villanueva D; Riiff TJ; Orts WJ; Pieja AJ; Lee CC
[Ad] Endereço:USDA-ARS-WRRC, Bioproducts Research Unit, 800 Buchanan Street, Albany, CA 94710, United States.
[Ti] Título:Methanotrophic production of polyhydroxybutyrate-co-hydroxyvalerate with high hydroxyvalerate content.
[So] Source:Int J Biol Macromol;87:302-7, 2016 Jun.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Type II methanotrophic bacteria are a promising production platform for PHA biopolymers. These bacteria are known to produce pure poly-3-hydroxybutyrate homopolymer (PHB). We isolated a strain, Methylocystis sp. WRRC1, that was capable of producing a wide range of polyhydroxybutyrate-co-hydroxyvalerate copolymers (PHB-co-HV) when co-fed methane and valerate or n-pentanol. The ratio of HB to HV monomer was directly related to the concentration of valeric acid in the PHA accumulation media. We observed increased incorporation of HV and total polymer under copper-free growth conditions. The PHB-co-HV copolymers produced had decreased melting temperatures and crystallinity compared with methanotroph-produced PHB.
[Mh] Termos MeSH primário: Methylocystaceae/metabolismo
Poliésteres/química
[Mh] Termos MeSH secundário: Transporte Biológico/efeitos dos fármacos
Cobre/farmacologia
Metano/metabolismo
Methylocystaceae/efeitos dos fármacos
Ácidos Pentanoicos/metabolismo
Pentanóis/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Pentanoic Acids); 0 (Pentanols); 0 (Polyesters); 0 (poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate)); 789U1901C5 (Copper); GZK92PJM7B (n-pentanoic acid); OP0UW79H66 (Methane)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160228
[St] Status:MEDLINE


  9 / 124 MEDLINE  
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[PMID]:26838340
[Au] Autor:Patel SK; Mardina P; Kim SY; Lee JK; Kim IW
[Ad] Endereço:Institute of SK-KU Biomaterials, Seoul 05029, Republic of Korea.
[Ti] Título:Biological Methanol Production by a Type II Methanotroph Methylocystis bryophila.
[So] Source:J Microbiol Biotechnol;26(4):717-24, 2016 Apr 28.
[Is] ISSN:1738-8872
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Methane (CH4) is the most abundant component in natural gas. To reduce its harmful environmental effect as a greenhouse gas, CH4 can be utilized as a low-cost feed for the synthesis of methanol by methanotrophs. In this study, several methanotrophs were examined for their ability to produce methanol from CH4; including Methylocella silvestris, Methylocystis bryophila, Methyloferula stellata, and Methylomonas methanica. Among these methanotrophs, M. bryophila exhibited the highest methanol production. The optimum process parameters aided in significant enhancement of methanol production up to 4.63 mM. Maximum methanol production was observed at pH 6.8, 30°C, 175 rpm, 100 mM phosphate buffer, 50 mM MgCl2 as a methanol dehydrogenase inhibitor, 50% CH4 concentration, 24 h of incubation, and 9 mg of dry cell mass ml(-1) inoculum load, respectively. Optimization of the process parameters, screening of methanol dehydrogenase inhibitors, and supplementation with formate resulted in significant improvements in methanol production using M. bryophila. This report suggests, for the first time, the potential of using M. bryophila for industrial methanol production from CH4.
[Mh] Termos MeSH primário: Produtos Biológicos
Metanol/metabolismo
Methylocystaceae/metabolismo
Microbiologia do Solo
[Mh] Termos MeSH secundário: Meios de Cultura/química
Formiatos/farmacologia
Microbiologia Industrial
Methylocystaceae/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Culture Media); 0 (Formates); 0YIW783RG1 (formic acid); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE
[do] DOI:10.4014/jmb.1601.01013


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[PMID]:26828022
[Au] Autor:Han SB; Su Y; Hu J; Wang RJ; Sun C; Wu D; Zhu XF; Wu M
[Ad] Endereço:1​College of Life Sciences, Zhejiang University, Hangzhou 310058, PRChina.
[Ti] Título:Terasakiella brassicae sp. nov., isolated from the wastewater of a pickle-processing factory, and emended descriptions of Terasakiella pusilla and the genus Terasakiella.
[So] Source:Int J Syst Evol Microbiol;66(4):1807-12, 2016 Apr.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, motile, polyhydroxybutyrate-accumulating, aerobic, S-shaped bacterium, designated B3T, was isolated from the wastewater of a pickle-processing factory. 16S rRNA gene sequence similarity analysis showed that it was most closely related to the type strain, Terasakiella pusilla (96.6% similarity). Strain B3T was able to grow at 4-40 °C (optimum 32-37 °C), pH 5.5-9.0 (optimum 6.5-7.5) and with 0.5-8% (w/v) NaCl present (optimum 1-2%, w/v). Chemotaxonomic analysis showed that the respiratory quinone was ubiquinone Q-10, the major fatty acids included C16:0, C18:1ω7c and C16:1ω7c and/or iso-C15:2-OH. The major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, aminophospholipid and three uncharacterized phospholipids. The genomic DNA G+C content of strain B3T was 42.3 mol%. The DNA-DNA relatedness value between B3T and T. pusilla DSM 9263T was 23.9%. On the basis of the phenotypic, chemotaxonomic and genotypic characteristics of strain B3T, it represents a novel species of the genus Terasakiella, for which the name Terasakiella brassicae sp. nov. is proposed. The type strain is B3T (=KCTC 42652T=CGMCC 1.15254T). Emended descriptions of T. pusilla and the genus Terasakiella are also presented.
[Mh] Termos MeSH primário: Methylocystaceae/classificação
Filogenia
Águas Residuais/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Indústria Alimentícia
Hidroxibutiratos/química
Methylocystaceae/genética
Methylocystaceae/isolamento & purificação
Dados de Sequência Molecular
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Hydroxybutyrates); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 0 (Waste Water); 1339-63-5 (Ubiquinone); I7T5V2W47R (Ubiquinone Q2)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160421
[Lr] Data última revisão:
160421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160202
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.000946



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