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Pesquisa : B03.660.075 [Categoria DeCS]
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[PMID]:29364604
[Au] Autor:Galach'yants AD; Bel'kova NL; Sukhanova EV; Romanovskaya VA; Gladka GV; Bedoshvili ED; Parfenova VV
[Ti] Título:[Diversity and Physiological and Biochemical Properties of Heterotrophic Bacteria. Isolated from Lake Baikal Neuston.]
[So] Source:Mikrobiologiia;85(5):568-579, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:For heterotrophic microorganisms (44 strains) isolated-from the surface film of Lake Baikal, iden- tification was carried out and their. physiological and biochemical characteristics were determined. Com- pared to the water column, diversity of cultured heterotrophs was low, indicating formation of stable micro- bial communities at the air-water interphase interface. Heterotrophic bacteria isolated from the surface mi- crolayer exhibited the enzymatic activity comparable to that for strains form other biofilm associations. Deinococcusfi6us strain NA202 'vas the most active component of the community, capable of utilization of the broadest spectrum of mono- and disaccharides,'sugars, and amino acids. This strain possessed the highest diversity of extracellular enzymes and was the most resistant to UV radiation. The physiological and bio- chemical properties of this strain may-be responsible for its adaptation to survival in extreme conditions of the surface microlayer. Our results improve our understanding of occurrence of UV-resistant strains in freshwater ecosystems.
[Mh] Termos MeSH primário: Deinococcus/metabolismo
Processos Heterotróficos/fisiologia
Lagos/microbiologia
Consórcios Microbianos/fisiologia
Filogenia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Alphaproteobacteria/classificação
Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Alphaproteobacteria/efeitos da radiação
Aminoácidos/metabolismo
Bacteroidetes/classificação
Bacteroidetes/genética
Bacteroidetes/isolamento & purificação
Bacteroidetes/efeitos da radiação
Betaproteobacteria/classificação
Betaproteobacteria/genética
Betaproteobacteria/isolamento & purificação
Betaproteobacteria/efeitos da radiação
Biodiversidade
Deinococcus/classificação
Deinococcus/isolamento & purificação
Deinococcus/efeitos da radiação
Deltaproteobacteria/classificação
Deltaproteobacteria/genética
Deltaproteobacteria/isolamento & purificação
Deltaproteobacteria/efeitos da radiação
Dissacarídeos/metabolismo
Ecossistema
Firmicutes/classificação
Firmicutes/genética
Firmicutes/isolamento & purificação
Firmicutes/efeitos da radiação
Gammaproteobacteria/classificação
Gammaproteobacteria/genética
Gammaproteobacteria/isolamento & purificação
Gammaproteobacteria/efeitos da radiação
Processos Heterotróficos/efeitos da radiação
Consórcios Microbianos/efeitos da radiação
Monossacarídeos/metabolismo
Proteobactérias/classificação
Proteobactérias/genética
Proteobactérias/isolamento & purificação
Proteobactérias/efeitos da radiação
Sibéria
Propriedades de Superfície
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Disaccharides); 0 (Monosaccharides); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:28581923
[Au] Autor:Boden R; Hutt LP; Rae AW
[Ad] Endereço:2​Sustainable Earth Institute, Plymouth University, Plymouth, UK 1​School of Biological and Marine Sciences, Plymouth University, Plymouth, UK.
[Ti] Título:Reclassification of Thiobacillus aquaesulis (Wood & Kelly, 1995) as Annwoodia aquaesulis gen. nov., comb. nov., transfer of Thiobacillus (Beijerinck, 1904) from the Hydrogenophilales to the Nitrosomonadales, proposal of Hydrogenophilalia class. nov. within the 'Proteobacteria', and four new families within the orders Nitrosomonadales and Rhodocyclales.
[So] Source:Int J Syst Evol Microbiol;67(5):1191-1205, 2017 May.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The genus Thiobacillus comprises four species with validly published names, of which Thiobacillus aquaesulis DSM 4255T (=ATCC 43788T) is the only species that can grow heterotrophically or mixotrophically - the rest being obligate autotrophs - and has a significant metabolic difference in not producing tetrathionate during the oxidation of thiosulfate during autotrophic growth. On the basis of this and differential chemotaxonomic properties and a 16S rRNA gene sequence similarity of 93.4 % to the type species Thiobacillus thioparus DSM 505T, we propose that it is moved to a novel genus, Annwoodia gen. nov., for which the type species is Annwoodia aquaesulis gen. nov., comb. nov. We confirm that the position of the genus Thiobacillus in the Betaproteobacteria falls within the Nitrosomonadales rather than the Hydrogenophilales as previously proposed. Within the Nitrosomonadales we propose the circumscription of genera to form the Thiobacilliaceae fam. nov. and the Sterolibacteriaceae fam. nov. We propose the merging of the family Methylophilaceae into the Nitrosomonadales, and that the Sulfuricellaceae be merged into the Gallionellaceae, leaving the orders Methylophilales and Sulfuricellales defunct. In the Rhodocyclales we propose the Azonexaceae fam. nov. and the Zoogloeaceae fam. nov. We also reject the Hydrogenophilales from the Betaproteobacteria on the basis of a very low 16S rRNA gene sequence similarity with the class-proper as well as physiological properties, forming the Hydrogenophilalia class. nov. in the 'Proteobacteria'. We provide emended descriptions of Thiobacillus, Hydrogenophilales, Hydrogenophilaceae, Nitrosomonadales, Gallionellaceae, Rhodocyclaceae and the Betaproteobacteria.
[Mh] Termos MeSH primário: Betaproteobacteria/classificação
Filogenia
Thiobacillus/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001927


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[PMID]:28531262
[Au] Autor:Liu Y; Vick-Majors TJ; Priscu JC; Yao T; Kang S; Liu K; Cong Z; Xiong J; Li Y
[Ad] Endereço:Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.
[Ti] Título:Biogeography of cryoconite bacterial communities on glaciers of the Tibetan Plateau.
[So] Source:FEMS Microbiol Ecol;93(6), 2017 Jun 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cryoconite holes, water-filled pockets containing biological and mineralogical deposits that form on glacier surfaces, play important roles in glacier mass balance, glacial geochemistry and carbon cycling. The presence of cryoconite material decreases surface albedo and accelerates glacier mass loss, a problem of particular importance in the rapidly melting Tibetan Plateau. No studies have addressed the microbial community composition of cryoconite holes and their associated ecosystem processes on Tibetan glaciers. To further enhance our understanding of these glacial ecosystems on the Tibetan Plateau and to examine their role in carbon cycling as the glaciers respond to climate change, we explored the bacterial communities within cryoconite holes associated with three climatically distinct Tibetan Plateau glaciers using Illumina sequencing of the V4 region of the 16S rRNA gene. Cryoconite bacterial communities were dominated by Cyanobacteria, Chloroflexi, Betaproteobacteria, Bacteroidetes and Actinobacteria. Cryoconite bacterial community composition varied according to their geographical locations, exhibiting significant differences among glaciers studied. Regional beta diversity was driven by the interaction between geographic distance and environmental variables; the latter contributed more than geographic distance to the variation in cryoconite microbial communities. Our study is the first to describe the regional-scale spatial variability and to identify the factors that drive regional variability of cryoconite bacterial communities on the Tibetan Plateau.
[Mh] Termos MeSH primário: Actinobacteria/genética
Bacteroidetes/genética
Betaproteobacteria/genética
Chloroflexi/genética
Cianobactérias/genética
Camada de Gelo/microbiologia
[Mh] Termos MeSH secundário: Actinobacteria/isolamento & purificação
Bacteroidetes/isolamento & purificação
Betaproteobacteria/isolamento & purificação
Chloroflexi/isolamento & purificação
Mudança Climática
Cianobactérias/isolamento & purificação
Ecossistema
Geografia
Sequenciamento de Nucleotídeos em Larga Escala
Consórcios Microbianos/genética
RNA Ribossômico 16S/genética
Tibet
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix072


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[PMID]:28521174
[Au] Autor:Vannini C; Sigona C; Hahn M; Petroni G; Fujishima M
[Ad] Endereço:Department of Biology, University of Pisa, Pisa, 56126, Italy. Electronic address: claudia.vannini@unipi.it.
[Ti] Título:High degree of specificity in the association between symbiotic betaproteobacteria and the host Euplotes (Ciliophora, Euplotia).
[So] Source:Eur J Protistol;59:124-132, 2017 Jun.
[Is] ISSN:1618-0429
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The Betaproteobacteria-Euplotes association is an obligatory symbiotic system involving a monophyletic group of ciliate species and two betaproteobacteria species which can be alternatively present. Recent data showed that this relationship has been established more than once and that several symbiont-substitution events took place, revealing a complex and intriguing evolutionary path. Due to the different evolutionary pathways followed by the different symbionts, each bacterial strain could have differentially evolved and/or lost functional traits. Therefore, we performed re-infection experiments, both by phagocytosis and by microinjection, to test the possible functional role of the different bacteria towards the ciliates. Our results confirm that the growth capacity of the host is indissolubly linked to the presence of its original symbionts. Results of the attempts of re-infection by phagocytosis showed that none of the bacteria is able to successfully colonize the host cytoplasm in this way, even if regularly ingested. Re-infection by microinjection succeed only in one case. Such results point to a high degree of specificity in the interactions between bacteria and Euplotes even after the invasion step. Due to a co-evolutive pathway of reciprocal adaptation, different degree of re-colonization ability could have been conserved by the different species and strains of the symbionts.
[Mh] Termos MeSH primário: Betaproteobacteria/fisiologia
Euplotes/microbiologia
Especificidade de Hospedeiro/fisiologia
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE


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[PMID]:28499263
[Au] Autor:Li J; Huang B; Wang Q; Li Y; Fang W; Yan D; Guo M; Cao A
[Ad] Endereço:Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Effect of fumigation with chloropicrin on soil bacterial communities and genes encoding key enzymes involved in nitrogen cycling.
[So] Source:Environ Pollut;227:534-542, 2017 Aug.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chloropicrin (CP) is a potential alternative for methyl bromide as a soil fumigant given that the use of methyl bromide has become limited. However, little is known about how fumigation with CP affects the condition of the soil microbial community. In this study, 16S rRNA amplicon sequencing and quantitative PCR were combined to investigate the effect of CP on soil bacterial community. In total, 938,922 effective reads were obtained from 18 samples and clustered into 58,662 operational taxonomic units at a similarity cut-off of 97%. Both approaches showed that the primary structure of bacterial community in soil did not significantly change at the phylum level after fumigation, but CP had a significant impact on the abundance of the bacterial microbiome that was recovered and identified. Additionally, bacterial community diversity decreased significantly, and there was a shift in the predominant populations. Staphylococcus, Actinomadura, Acinetobacter and Streptomyces significantly decreased in number or disappeared, and Bacteroides, Lachnoclostridium, Pseudoalteromonas, Colwellia, Idiomarina and Cobetia became the new predominant populations. In addition, some species associated with biodegradation, such as Sphingomonas spp. and Rhodococcus spp., significantly increased in number. The abundance of ammonia-oxidizing archaea (AOA) were significantly inhibited, yet the abundance of ammonia-oxidizing bacteria (AOB) significantly increased, and denitrification was significantly promoted. These changes in bacterial flora can considerably impact soil function and health and lead to negative effects on the environment surrounding fumigated soils, indicating the need for proactive risk management. Our study provides useful information for environmental safety assessments of CP in China.
[Mh] Termos MeSH primário: Fumigação/métodos
Hidrocarbonetos Clorados/toxicidade
Ciclo do Nitrogênio/genética
Microbiologia do Solo
[Mh] Termos MeSH secundário: Amônia/metabolismo
Archaea/metabolismo
Bactérias/metabolismo
Betaproteobacteria/genética
Biodegradação Ambiental
China
Genes Bacterianos
Nitrificação
Nitrogênio/metabolismo
Ciclo do Nitrogênio/efeitos dos fármacos
Oxirredução
Praguicidas/metabolismo
RNA Ribossômico 16S
Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrocarbons, Chlorinated); 0 (Pesticides); 0 (RNA, Ribosomal, 16S); 0 (Soil); 7664-41-7 (Ammonia); I4JTX7Z7U2 (chloropicrin); N762921K75 (Nitrogen)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE


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[PMID]:28498940
[Au] Autor:Li Q; Song A; Peng W; Jin Z; Müller WEG; Wang X
[Ad] Endereço:Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, PR China.
[Ti] Título:Contribution of aerobic anoxygenic phototrophic bacteria to total organic carbon pool in aquatic system of subtropical karst catchments, Southwest China: evidence from hydrochemical and microbiological study.
[So] Source:FEMS Microbiol Ecol;93(6), 2017 Jun 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aerobic anoxygenic phototrophic bacteria may play a particular role in carbon cycling of aquatic systems. However, little is known about the interaction between aerobic anoxygenic phototrophic bacteria and hydrochemistry in groundwater-surface water exchange systems of subtropical karst catchments. We carried out a detailed study on the abundance of aerobic anoxygenic phototrophic bacteria and bacterioplankton, hydrochemistry and taxonomy of bacterioplankton in the Maocun watershed, Southwest China, an area with karst geological background. Our results revealed that bacteria are the important contributors to total organic carbon source/sequestration in the groundwater-surface water of this area. The aerobic anoxygenic phototrophic bacteria, including ß-Proteobacteria, also appear in the studied water system. In addition to that, the genus Polynucleobacter of the phototropic ß-Proteobacteria shows a close link with those sampling sites by presenting bacterial origin organic carbon on CCA biplot and is found to be positively correlated with total nitrogen, dissolved oxygen and pH (r = 0.860, 0.747 and 0.813, respectively) in the Maocun watershed. The results suggest that Polynucleobacter might be involved in the production of organic carbon and might act as the negative feedback on global warming.
[Mh] Termos MeSH primário: Betaproteobacteria/metabolismo
Carbono/metabolismo
Água Subterrânea/microbiologia
Processos Fototróficos/fisiologia
[Mh] Termos MeSH secundário: Organismos Aquáticos/metabolismo
Bactérias Aeróbias/classificação
Bactérias Aeróbias/genética
Bactérias Aeróbias/metabolismo
Betaproteobacteria/classificação
Betaproteobacteria/genética
Carbono/análise
China
Aquecimento Global
Nitrogênio/análise
Oxigênio/metabolismo
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 7440-44-0 (Carbon); N762921K75 (Nitrogen); S88TT14065 (Oxygen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix065


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[PMID]:28477571
[Au] Autor:Islam MS; Zhang Y; Dong S; McPhedran KN; Rashed EM; El-Shafei MM; Noureldin AM; Gamal El-Din M
[Ad] Endereço:Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, Canada.
[Ti] Título:Dynamics of microbial community structure and nutrient removal from an innovative side-stream enhanced biological phosphorus removal process.
[So] Source:J Environ Manage;198(Pt 1):300-307, 2017 Aug 01.
[Is] ISSN:1095-8630
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Biological phosphorous (P) and nitrogen (N) removal from municipal wastewater was studied using an innovative anoxic-aerobic-anaerobic side-stream treatment system. The impact of influent water quality including chemical oxygen demand (COD), ammonium and orthophosphate concentrations on the reactor performance was evaluated. The results showed the system was very effective at removing both COD (>88%) and NH -N (>96%) despite varying influent concentrations of COD, NH -N, and total PO -P. In contrast, it was found that the removal of P was sensitive to influent NH -N and PO -P concentrations. The maximum PO -P removal of 79% was achieved with the lowest influent NH -N and PO -P concentration. Quantitative PCR (qPCR) assays showed a high abundance and diversity of phosphate accumulating organisms (PAO), nitrifiers and denitrifiers. The MiSeq microbial community structure analysis showed that the Proteobacteria (especially ß-Proteobacteria, and γ-Proteobacteria) were the dominant in all reactors. Further analysis of the bacteria indicated the presence of diverse PAO genera including Candidatus Accumulibacter phosphatis, Tetrasphaera, and Rhodocyclus, and the denitrifying PAO (DPAO) genus Dechloromonas. Interestingly, no glycogen accumulating organisms (GAOs) were detected in any of the reactors, suggesting the advantage of proposed process in term of PAO selection for enhanced P removal compared with conventional main-stream processes.
[Mh] Termos MeSH primário: Reatores Biológicos
Fósforo
[Mh] Termos MeSH secundário: Betaproteobacteria
Rios
Águas Residuais
Purificação da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water); 27YLU75U4W (Phosphorus)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170507
[St] Status:MEDLINE


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[PMID]:28390702
[Au] Autor:Crampon M; Cébron A; Portet-Koltalo F; Uroz S; Le Derf F; Bodilis J
[Ad] Endereço:COBRA UMR CNRS 6014, Université de Rouen-Normandie, 55 rue saint Germain, 27000 Evreux, France; Laboratoire de Microbiologie Signaux et Microenvironnement, EA 4312, Université de Rouen, 76821 Mont Saint Aignan, France.
[Ti] Título:Low effect of phenanthrene bioaccessibility on its biodegradation in diffusely contaminated soil.
[So] Source:Environ Pollut;225:663-673, 2017 Jun.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study focused on the role of bioaccessibility in the phenanthrene (PHE) biodegradation in diffusely contaminated soil, by combining chemical and microbiological approaches. First, we determined PHE dissipation rates and PHE sorption/desorption isotherms for two soils (PPY and Pv) presenting similar chronic PAH contamination, but different physico-chemical properties. Our results revealed that the PHE dissipation rate was significantly higher in the Pv soil compared to the PPY soil, while PHE sorption/desorption isotherms were similar. Interestingly, increases of PHE desorption and potentially of PHE bioaccessibility were observed for both soils when adding rhamnolipids (biosurfactants produced by Pseudomonas aeruginosa). Second, using C-PHE incubated in the same soils, we analyzed the PHE degrading bacterial communities. The combination of stable isotope probing (DNA-SIP) and 16S rRNA gene pyrosequencing revealed that Betaproteobacteria were the main PHE degraders in the Pv soil, while a higher bacterial diversity (Alpha-, Beta-, Gammaproteobacteria and Actinobacteria) was involved in PHE degradation in the PPY soil. The amendment of biosurfactants commonly used in biostimulation methods (i.e. rhamnolipids) to the two soils clearly modified the PHE sorption/desorption isotherms, but had no significant impact on PHE degradation rates and PHE-degraders identity. These results demonstrated that increasing the bioaccessibility of PHE has a low impact on its degradation and on the functional populations involved in this degradation.
[Mh] Termos MeSH primário: Fenantrenos/metabolismo
Microbiologia do Solo
Poluentes do Solo/metabolismo
[Mh] Termos MeSH secundário: Betaproteobacteria/genética
Biodegradação Ambiental
Gammaproteobacteria
Glicolipídeos
Fenantrenos/química
RNA Ribossômico 16S
Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycolipids); 0 (Phenanthrenes); 0 (RNA, Ribosomal, 16S); 0 (Soil); 0 (Soil Pollutants); 0 (rhamnolipid); 448J8E5BST (phenanthrene)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170410
[St] Status:MEDLINE


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[PMID]:28335808
[Au] Autor:Wu X; Pedersen K; Edlund J; Eriksson L; Åström M; Andersson AF; Bertilsson S; Dopson M
[Ad] Endereço:Centre for Ecology and Evolution in Microbial Model Systems (EEMiS), Linnaeus University, 391 82, Kalmar, Sweden.
[Ti] Título:Potential for hydrogen-oxidizing chemolithoautotrophic and diazotrophic populations to initiate biofilm formation in oligotrophic, deep terrestrial subsurface waters.
[So] Source:Microbiome;5(1):37, 2017 Mar 23.
[Is] ISSN:2049-2618
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Deep terrestrial biosphere waters are separated from the light-driven surface by the time required to percolate to the subsurface. Despite biofilms being the dominant form of microbial life in many natural environments, they have received little attention in the oligotrophic and anaerobic waters found in deep bedrock fractures. This study is the first to use community DNA sequencing to describe biofilm formation under in situ conditions in the deep terrestrial biosphere. RESULTS: In this study, flow cells were attached to boreholes containing either "modern marine" or "old saline" waters of different origin and degree of isolation from the light-driven surface of the earth. Using 16S rRNA gene sequencing, we showed that planktonic and attached populations were dissimilar while gene frequencies in the metagenomes suggested that hydrogen-fed, carbon dioxide- and nitrogen-fixing populations were responsible for biofilm formation across the two aquifers. Metagenome analyses further suggested that only a subset of the populations were able to attach and produce an extracellular polysaccharide matrix. Initial biofilm formation is thus likely to be mediated by a few bacterial populations which were similar to Epsilonproteobacteria, Deltaproteobacteria, Betaproteobacteria, Verrucomicrobia, and unclassified bacteria. CONCLUSIONS: Populations potentially capable of attaching to a surface and to produce extracellular polysaccharide matrix for attachment were identified in the terrestrial deep biosphere. Our results suggest that the biofilm populations were taxonomically distinct from the planktonic community and were enriched in populations with a chemolithoautotrophic and diazotrophic metabolism coupling hydrogen oxidation to energy conservation under oligotrophic conditions.
[Mh] Termos MeSH primário: Betaproteobacteria/genética
Biofilmes/classificação
Crescimento Quimioautotrófico/genética
Deltaproteobacteria/genética
Epsilonproteobacteria/genética
Fixação de Nitrogênio/genética
Verrucomicrobia/genética
[Mh] Termos MeSH secundário: Betaproteobacteria/classificação
Crescimento Quimioautotrófico/fisiologia
Deltaproteobacteria/classificação
Epsilonproteobacteria/classificação
Ambientes Extremos
Água Subterrânea/microbiologia
Hidrogênio/química
Metagenoma
Fixação de Nitrogênio/fisiologia
Oceanos e Mares
Oxirredução
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Verrucomicrobia/classificação
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 7YNJ3PO35Z (Hydrogen)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1186/s40168-017-0253-y


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[PMID]:28334155
[Au] Autor:Dall'Agnol RF; Bournaud C; de Faria SM; Béna G; Moulin L; Hungria M
[Ad] Endereço:Soil Biotechnology Laboratory, Embrapa Soja, C.P. 231, 86001-970, Londrina, PR, Brazil.
[Ti] Título:Genetic diversity of symbiotic Paraburkholderia species isolated from nodules of Mimosa pudica (L.) and Phaseolus vulgaris (L.) grown in soils of the Brazilian Atlantic Forest (Mata Atlântica).
[So] Source:FEMS Microbiol Ecol;93(4), 2017 Apr 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Some species of the genus Paraburkholderia that are able to nodulate and fix nitrogen in symbiosis with legumes are called ß-rhizobia and represent a group of ecological and biotechnological importance. We used Mimosa pudica and Phaseolus vulgaris to trap 427 rhizobial isolates from rhizospheric soil of Mimoseae trees in the Brazilian Atlantic Forest. Eighty-four representative strains were selected according to the 16S rRNA haplotypes and taxonomically characterized using a concatenated 16S rRNA-recA phylogeny. Most strains were assembled in the genus Paraburkholderia, including Paraburkholderia sabiae and Pa. nodosa. Mesorhizobium (α-rhizobia) and Cupriavidus (ß-rhizobia) were also isolated, but in smaller proportions. Multilocus sequence analysis and BOX-PCR analyses indicated that six clusters of Paraburkholderia represent potential new species. In the phylogenetic analysis of the nodC gene, the majority of the strains were positioned in the same groups as in the 16S rRNA-recA tree, indicative of stability and vertical inheritance, but we also identified horizontal transfer of nodC in Pa. sabiae. All α- and ß-rhizobial species were trapped by both legumes, although preferences of the host plants for specific rhizobial species have been observed.
[Mh] Termos MeSH primário: Betaproteobacteria/genética
Florestas
Variação Genética
Mimosa/microbiologia
Phaseolus/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Brasil
Cupriavidus/classificação
RNA Ribossômico 16S/genética
Rhizobium/genética
Solo
Microbiologia do Solo
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); 0 (Soil)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix027



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