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[PMID]: 29364604 [Au] Autor: Galach'yants AD; Bel'kova NL; Sukhanova EV; Romanovskaya VA; Gladka GV; Bedoshvili ED; Parfenova VV [Ti] Título: [Diversity and Physiological and Biochemical Properties of Heterotrophic Bacteria. Isolated from Lake Baikal Neuston.] [So] Source: Mikrobiologiia;85(5):568-579, 2016 Sep. [Is] ISSN: 0026-3656 [Cp] País de publicação: Russia (Federation) [La] Idioma: rus [Ab] Resumo: For heterotrophic microorganisms (44 strains) isolated-from the surface film of Lake Baikal, iden- tification was carried out and their. physiological and biochemical characteristics were determined. Com- pared to the water column, diversity of cultured heterotrophs was low, indicating formation of stable micro- bial communities at the air-water interphase interface. Heterotrophic bacteria isolated from the surface mi- crolayer exhibited the enzymatic activity comparable to that for strains form other biofilm associations. Deinococcusfi6us strain NA202 'vas the most active component of the community, capable of utilization of the broadest spectrum of mono- and disaccharides,'sugars, and amino acids. This strain possessed the highest diversity of extracellular enzymes and was the most resistant to UV radiation. The physiological and bio- chemical properties of this strain may-be responsible for its adaptation to survival in extreme conditions of the surface microlayer. Our results improve our understanding of occurrence of UV-resistant strains in freshwater ecosystems. [Mh] Termos MeSH primário: Deinococcus/metabolismo Processos Heterotróficos/fisiologia Lagos/microbiologia Consórcios Microbianos/fisiologia Filogenia RNA Ribossômico 16S/genética [Mh] Termos MeSH secundário: Alphaproteobacteria/classificação Alphaproteobacteria/genética Alphaproteobacteria/isolamento & purificação Alphaproteobacteria/efeitos da radiação Aminoácidos/metabolismo Bacteroidetes/classificação Bacteroidetes/genética Bacteroidetes/isolamento & purificação Bacteroidetes/efeitos da radiação Betaproteobacteria/classificação Betaproteobacteria/genética Betaproteobacteria/isolamento & purificação Betaproteobacteria/efeitos da radiação Biodiversidade Deinococcus/classificação Deinococcus/isolamento & purificação Deinococcus/efeitos da radiação Deltaproteobacteria/classificação Deltaproteobacteria/genética Deltaproteobacteria/isolamento & purificação Deltaproteobacteria/efeitos da radiação Dissacarídeos/metabolismo Ecossistema Firmicutes/classificação Firmicutes/genética Firmicutes/isolamento & purificação Firmicutes/efeitos da radiação Gammaproteobacteria/classificação Gammaproteobacteria/genética Gammaproteobacteria/isolamento & purificação Gammaproteobacteria/efeitos da radiação Processos Heterotróficos/efeitos da radiação Consórcios Microbianos/efeitos da radiação Monossacarídeos/metabolismo Proteobactérias/classificação Proteobactérias/genética Proteobactérias/isolamento & purificação Proteobactérias/efeitos da radiação Sibéria Propriedades de Superfície Raios Ultravioleta [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Amino Acids); 0 (Disaccharides); 0 (Monosaccharides); 0 (RNA, Ribosomal, 16S) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180208 [Lr] Data última revisão: 180208 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180125 [St] Status: MEDLINE

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[PMID]: 29364603 [Au] Autor: Kashkak ES; Bel'kova NL; Danilova EV; Dagurova OP; Namsaraev BB; Gorlenko VM [Ti] Título: Phylogenetic and Functional Prokaryotic Diversity in the Hoito-Gol Mesothermal Mineral Spring (Eastern Savan Mountains. Buryat Renuhlie). [So] Source: Mikrobiologiia;85(5):555-567, 2016 Sep. [Is] ISSN: 0026-3656 [Cp] País de publicação: Russia (Federation) [La] Idioma: eng [Ab] Resumo: High-throughput sequencing was used for comparative analysis of microbial communities of the water and mat from the Hoito-Gol mesothermal mineral sulfide spring (Eastern Sayan Mountains, Buryat Republic). Activity of microbial communities was determined. While both spring biotopes were dominated by members of three bacterial phyla, Proteobacteria, Bacteroidetes, and Firmicutes, they differed drastically in the composition of predominant phylotypes (at the genus level). In the water, the organisms wide spread in aquatic'environments were predominant, mostly aerobic chemoorganotrophs of the generaAcinetobacter, Pe- dobacter, and Flavobacterium. In the microbial mat,;the organisms actively involved in the sulfur cycle predominated, including sulfur-reducing bacteria Sulfurospirillum, sulfate-reducing deltaproteobacteria, sulfur- oxidizing chemoautotrophic bacteria, anoxygenic phototrophic bacteria of,the phyla Chloroflexi and Chloro- bi, as well as purple bacteria belonging to the Q-, P--, and y-Proteobacteria. Microbial mats of the spring exhibited higher phylogenetic diversity compared to high-temperature mats containing photosynthetic microorganisms. [Mh] Termos MeSH primário: Água Subterrânea/microbiologia Consórcios Microbianos/fisiologia Águas Minerais/microbiologia Filogenia RNA Ribossômico 16S/genética [Mh] Termos MeSH secundário: Bacteroidetes/classificação Bacteroidetes/genética Bacteroidetes/isolamento & purificação Biodiversidade Campylobacteraceae/classificação Campylobacteraceae/genética Campylobacteraceae/isolamento & purificação Deltaproteobacteria/classificação Deltaproteobacteria/genética Deltaproteobacteria/isolamento & purificação Ecossistema Firmicutes/classificação Firmicutes/genética Firmicutes/isolamento & purificação Metano/química Metano/metabolismo Proteobactérias/classificação Proteobactérias/genética Proteobactérias/isolamento & purificação Estações do Ano Sibéria [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Mineral Waters); 0 (RNA, Ribosomal, 16S); OP0UW79H66 (Methane) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180208 [Lr] Data última revisão: 180208 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180125 [St] Status: MEDLINE

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[PMID]: 28820122 [Au] Autor: Watanabe M; Higashioka Y; Kojima H; Fukui M [Ad] Endereço: 1​The Institute of Low Temperature Science, Hokkaido University, Kita-19, Nishi-8, Kita-ku, Sapporo 060-0819, Japan 2​Postdoctoral Research Fellow of the Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo 102-8471, Japan. [Ti] Título: Desulfosarcina widdelii sp. nov. and Desulfosarcina alkanivorans sp. nov., hydrocarbon-degrading sulfate-reducing bacteria isolated from marine sediment and emended description of the genus Desulfosarcina. [So] Source: Int J Syst Evol Microbiol;67(8):2994-2997, 2017 Aug. [Is] ISSN: 1466-5034 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: In previous studies, two hydrocarbon-degrading sulfate-reducing bacteria, strains PP31T and PL12T, were obtained from oil-polluted marine sediments of Shuaiba, Kuwait. They had been reported as organisms capable of anaerobic degradation of p-xylene and n-alkanes, respectively. The 16S rRNA gene sequence of strain PP31T showed 98.8 % sequence similarities to that of Desulfosarcina variabilis'Montpellier'T. Strains PL12T had 97.8 % of sequence similarity to Desulfosarcina ovata oXys1T. They both have been partially characterized, but not been validly published as new species of the genus Desulfosarcina. In this study, additional characterizations of these strains were made to describe them as two new species of the genus Desulfosarcina. Major cellular fatty acids of strain PP31T were C15 : 0 (25.9 %) and anteiso-C15 : 0 (22.3 %), whereas those of strain PL12T were C15 : 0 (21.3 %), C16 : 0 (17.8 %) and anteiso-15 : 0 (11.6 %). The phylogenetic tree based on 16S rRNA gene revealed that these isolates should not be classified as any of the known species in the genus Desulfosarcina. On the basis of phenotypic and phylogenetic analyses, these two sulfate reducers are proposed to form two novel species of the genus Desulfosarcina : Desulfosarcina widdelii sp. nov. (PP31T=JCM 31729T=DSM 103921T) and Desulfosarcina alkanivorans sp. nov. (PL12T=JCM 31728T=DSM 103901T). In addition, emended description of the genus Desulfosarcina is presented in this study. [Mh] Termos MeSH primário: Deltaproteobacteria/classificação Sedimentos Geológicos/microbiologia Hidrocarbonetos/metabolismo Filogenia Água do Mar/microbiologia [Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana Composição de Bases DNA Bacteriano/genética Deltaproteobacteria/genética Deltaproteobacteria/isolamento & purificação Ácidos Graxos/química Kuweit RNA Ribossômico 16S/genética Análise de Sequência de DNA Bactérias Redutoras de Enxofre/classificação Bactérias Redutoras de Enxofre/genética Bactérias Redutoras de Enxofre/isolamento & purificação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Hydrocarbons); 0 (RNA, Ribosomal, 16S) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170918 [Lr] Data última revisão: 170918 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170819 [St] Status: MEDLINE [do] DOI: 10.1099/ijsem.0.002062

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[PMID]: 28771119 [Au] Autor: Hahn MW; Schmidt J; Koll U; Rohde M; Verbarg S; Pitt A; Nakai R; Naganuma T; Lang E [Ad] Endereço: 1​Research Institute for Limnology, University of Innsbruck, Mondseestrasse 9, 5310 Mondsee, Austria. [Ti] Título: Silvanigrella aquatica gen. nov., sp. nov., isolated from a freshwater lake, description of Silvanigrellaceae fam. nov. and Silvanigrellales ord. nov., reclassification of the order Bdellovibrionales in the class Oligoflexia, reclassification of the families Bacteriovoracaceae and Halobacteriovoraceae in the new order Bacteriovoracales ord. nov., and reclassification of the family Pseudobacteriovoracaceae in the order Oligoflexales. [So] Source: Int J Syst Evol Microbiol;67(8):2555-2568, 2017 Aug. [Is] ISSN: 1466-5034 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The unusual chemo-organoheterotrophic proteobacterial strain MWH-Nonnen-W8redT was isolated from a lake located in the Black Forest (Schwarzwald), Germany, by using the filtration-acclimatization method. Phylogenetic analyses based on the 16S rRNA gene sequence of the strain could not provide clear hints on classification of the strain in one of the current classes of the phylum Proteobacteria. Whole-genome sequencing resulted in a genome size of 3.5 Mbp and revealed a quite low DNA G+C content of 32.6 mol%. In-depth phylogenetic analyses based on alignments of 74 protein sequences of a phylogenetically broad range of taxa suggested assignment of the strain to a new order of the class Oligoflexia. These analyses also suggested that the order Bdellovibrionales should be transferred from the class Deltaproteobacteria to the class Oligoflexia, that this order should be split into two orders, and that the family Pseudobacteriovoracaceae should be transferred from the order Bdellovibrionales to the order Oligoflexales. We propose to establish for strain MWH-Nonnen-W8redT (=DSM 23856T=CCUG 58639T) the novel species and genus Silvanigrella aquatica gen. nov., sp. nov. to be placed in the new family Silvanigrellaceae fam. nov. of the new order Silvanigrellales ord. nov. [Mh] Termos MeSH primário: Deltaproteobacteria/classificação Lagos/microbiologia Filogenia [Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana Composição de Bases DNA Bacteriano/genética Deltaproteobacteria/genética Deltaproteobacteria/isolamento & purificação Alemanha RNA Ribossômico 16S/genética Análise de Sequência de DNA [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171006 [Lr] Data última revisão: 171006 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170804 [St] Status: MEDLINE [do] DOI: 10.1099/ijsem.0.001965

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[PMID]: 28335808 [Au] Autor: Wu X; Pedersen K; Edlund J; Eriksson L; Åström M; Andersson AF; Bertilsson S; Dopson M [Ad] Endereço: Centre for Ecology and Evolution in Microbial Model Systems (EEMiS), Linnaeus University, 391 82, Kalmar, Sweden. [Ti] Título: Potential for hydrogen-oxidizing chemolithoautotrophic and diazotrophic populations to initiate biofilm formation in oligotrophic, deep terrestrial subsurface waters. [So] Source: Microbiome;5(1):37, 2017 Mar 23. [Is] ISSN: 2049-2618 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Deep terrestrial biosphere waters are separated from the light-driven surface by the time required to percolate to the subsurface. Despite biofilms being the dominant form of microbial life in many natural environments, they have received little attention in the oligotrophic and anaerobic waters found in deep bedrock fractures. This study is the first to use community DNA sequencing to describe biofilm formation under in situ conditions in the deep terrestrial biosphere. RESULTS: In this study, flow cells were attached to boreholes containing either "modern marine" or "old saline" waters of different origin and degree of isolation from the light-driven surface of the earth. Using 16S rRNA gene sequencing, we showed that planktonic and attached populations were dissimilar while gene frequencies in the metagenomes suggested that hydrogen-fed, carbon dioxide- and nitrogen-fixing populations were responsible for biofilm formation across the two aquifers. Metagenome analyses further suggested that only a subset of the populations were able to attach and produce an extracellular polysaccharide matrix. Initial biofilm formation is thus likely to be mediated by a few bacterial populations which were similar to Epsilonproteobacteria, Deltaproteobacteria, Betaproteobacteria, Verrucomicrobia, and unclassified bacteria. CONCLUSIONS: Populations potentially capable of attaching to a surface and to produce extracellular polysaccharide matrix for attachment were identified in the terrestrial deep biosphere. Our results suggest that the biofilm populations were taxonomically distinct from the planktonic community and were enriched in populations with a chemolithoautotrophic and diazotrophic metabolism coupling hydrogen oxidation to energy conservation under oligotrophic conditions. [Mh] Termos MeSH primário: Betaproteobacteria/genética Biofilmes/classificação Crescimento Quimioautotrófico/genética Deltaproteobacteria/genética Epsilonproteobacteria/genética Fixação de Nitrogênio/genética Verrucomicrobia/genética [Mh] Termos MeSH secundário: Betaproteobacteria/classificação Crescimento Quimioautotrófico/fisiologia Deltaproteobacteria/classificação Epsilonproteobacteria/classificação Ambientes Extremos Água Subterrânea/microbiologia Hidrogênio/química Metagenoma Fixação de Nitrogênio/fisiologia Oceanos e Mares Oxirredução Filogenia RNA Ribossômico 16S/genética Análise de Sequência de DNA Verrucomicrobia/classificação Microbiologia da Água [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (RNA, Ribosomal, 16S); 7YNJ3PO35Z (Hydrogen) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170626 [Lr] Data última revisão: 170626 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170325 [St] Status: MEDLINE [do] DOI: 10.1186/s40168-017-0253-y

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[PMID]: 28334283 [Au] Autor: Foght JM; Gieg LM; Siddique T [Ad] Endereço: Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E9. [Ti] Título: The microbiology of oil sands tailings: past, present, future. [So] Source: FEMS Microbiol Ecol;93(5), 2017 May 01. [Is] ISSN: 1574-6941 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Surface mining of enormous oil sands deposits in northeastern Alberta, Canada since 1967 has contributed greatly to Canada's economy but has also received negative international attention due largely to environmental concerns and challenges. Not only have microbes profoundly affected the composition and behavior of this petroleum resource over geological time, they currently influence the management of semi-solid tailings in oil sands tailings ponds (OSTPs) and tailings reclamation. Historically, microbial impacts on OSTPs were generally discounted, but next-generation sequencing and biogeochemical studies have revealed unexpectedly diverse indigenous communities and expanded our fundamental understanding of anaerobic microbial functions. OSTPs that experienced different processing and management histories have developed distinct microbial communities that influence the behavior and reclamation of the tailings stored therein. In particular, the interactions of Deltaproteobacteria and Firmicutes with methanogenic archaea impact greenhouse gas emissions, sulfur cycling, pore water toxicity, sediment biogeochemistry and densification, water usage and the trajectory of long-term mine waste reclamation. This review summarizes historical data; synthesizes current understanding of microbial diversity and activities in situ and in vitro; predicts microbial effects on tailings remediation and reclamation; and highlights knowledge gaps for future research. [Mh] Termos MeSH primário: Archaea/metabolismo Deltaproteobacteria/metabolismo Recuperação e Remediação Ambiental/métodos Firmicutes/metabolismo Sedimentos Geológicos/microbiologia Campos de Petróleo e Gás/microbiologia Petróleo/metabolismo [Mh] Termos MeSH secundário: Alberta Biodegradação Ambiental Canadá Sedimentos Geológicos/química Efeito Estufa Hidrocarbonetos/metabolismo Metano/biossíntese Mineração Oxirredução Petróleo/microbiologia Tanques/microbiologia Sulfatos/metabolismo Enxofre/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Hydrocarbons); 0 (Petroleum); 0 (Sulfates); 70FD1KFU70 (Sulfur); OP0UW79H66 (Methane) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170922 [Lr] Data última revisão: 170922 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170324 [St] Status: MEDLINE [do] DOI: 10.1093/femsec/fix034

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[PMID]: 28211847 [Au] Autor: Hamann E; Tegetmeyer HE; Riedel D; Littmann S; Ahmerkamp S; Chen J; Hach PF; Strous M [Ad] Endereço: Department of Geoscience, University of Calgary, Calgary, AB, Canada. [Ti] Título: Syntrophic linkage between predatory Carpediemonas and specific prokaryotic populations. [So] Source: ISME J;11(5):1205-1217, 2017 May. [Is] ISSN: 1751-7370 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Most anoxic environments are populated by small (<10 µm) heterotrophic eukaryotes that prey on different microbial community members. How predatory eukaryotes engage in beneficial interactions with other microbes has rarely been investigated so far. Here, we studied an example of such an interaction by cultivating the anerobic marine flagellate, Carpediemonas frisia sp. nov. (supergroup Excavata), with parts of its naturally associated microbiome. This microbiome consisted of so far uncultivated members of the Deltaproteobacteria, Bacteroidetes, Firmicutes, Verrucomicrobia and Nanoarchaeota. Using genome and transcriptome informed metabolic network modeling, we showed that Carpediemonas stimulated prokaryotic growth through the release of predigested biomolecules such as proteins, sugars, organic acids and hydrogen. Transcriptional gene activities suggested niche separation between biopolymer degrading Bacteroidetes, monomer utilizing Firmicutes and Nanoarchaeota and hydrogen oxidizing Deltaproteobacteria. An efficient metabolite exchange between the different community members appeared to be promoted by the formation of multispecies aggregates. Physiological experiments showed that Carpediemonas could also benefit from an association to these aggregates, as it facilitated the removal of inhibiting metabolites and increased the availability of prey bacteria. Taken together, our results provide a framework to understand how predatory microbial eukaryotes engage, across trophic levels, in beneficial interactions with specific prokaryotic populations. [Mh] Termos MeSH primário: Eucariotos/metabolismo Microbiota [Mh] Termos MeSH secundário: Bactérias/metabolismo Deltaproteobacteria/metabolismo Eucariotos/genética Eucariotos/fisiologia Redes e Vias Metabólicas/genética Interações Microbianas Nanoarchaeota/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170922 [Lr] Data última revisão: 170922 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170218 [St] Status: MEDLINE [do] DOI: 10.1038/ismej.2016.197

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[PMID]: 28198083 [Au] Autor: Liu P; Conrad R [Ad] Endereço: Department of Biogeochemistry, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany. [Ti] Título: Syntrophobacteraceae-affiliated species are major propionate-degrading sulfate reducers in paddy soil. [So] Source: Environ Microbiol;19(4):1669-1686, 2017 Apr. [Is] ISSN: 1462-2920 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Methane is an important greenhouse gas and propionate is next to acetate the main intermediate (average 23%) of the carbon flow to CH in paddy fields. Sulfate (e.g., gypsum) application can reduce CH emissions up to 70%. However, the effect of gypsum application on propionate degradation and the microbial communities involved are not well understood. Therefore, we studied propionate-dependent sulfate reduction in anoxic microcosms of paddy soils from Italy and the Philippines, combining 16S rRNA and dissimilatory sulfite reductase (dsrB) gene profiling and co-occurrence network analysis. Sulfate was stoichiometrically reduced in treatments with propionate addition, while CH production was partially suppressed. Methane production but not sulfate reduction were suppressed and acetate accumulated after addition of methyl fluoride or fluoroacetate. With methyl fluoride in the presence of sulfate, the accumulated acetate was consumed after the depletion of propionate. Simultaneously, the relative abundances of Syntrophobacteraceae and Desulfovibrionaceae were significantly enhanced, while fluoroacetate repressed Desulfobulbaceae in both soils. Syntrophobacter 16S rRNA and dsrB gene copy numbers were also remarkably increased with gypsum amendment. Network analysis of both 16S rRNA and dsrB genes illustrated a strong co-occurrence of operational taxonomic units belonging to Syntrophobacteraceae, Desulfovibrionaceae and Desulfobulbaceae. In summary, Syntrophobacteraceae affiliated species were identified as the major propionate-dependent sulfate reducers in paddy soil. They (together with Desulfobulbaceae) oxidized propionate directly to acetate and CO , or coupled the oxidation syntrophically to H /formate-utilizing Desulfovibrionaceae. The transiently accumulating acetate was preferentially consumed by acetoclastic Methanosarcinaceae. [Mh] Termos MeSH primário: Deltaproteobacteria/metabolismo Propionatos/metabolismo Microbiologia do Solo [Mh] Termos MeSH secundário: Carbono/metabolismo Deltaproteobacteria/genética Sulfito de Hidrogênio Redutase/metabolismo Itália Metano/metabolismo Oxirredução RNA Ribossômico 16S/genética Sulfatos/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Propionates); 0 (RNA, Ribosomal, 16S); 0 (Sulfates); 7440-44-0 (Carbon); EC 1.8.99.3 (Hydrogensulfite Reductase); OP0UW79H66 (Methane) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170821 [Lr] Data última revisão: 170821 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170216 [St] Status: MEDLINE [do] DOI: 10.1111/1462-2920.13698

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[PMID]: 28168783 [Au] Autor: Wu R; Wilton R; Cuff ME; Endres M; Babnigg G; Edirisinghe JN; Henry CS; Joachimiak A; Schiffer M; Pokkuluri PR [Ad] Endereço: Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne, Illinois, 60439. [Ti] Título: A novel signal transduction protein: Combination of solute binding and tandem PAS-like sensor domains in one polypeptide chain. [So] Source: Protein Sci;26(4):857-869, 2017 Apr. [Is] ISSN: 1469-896X [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: We report the structural and biochemical characterization of a novel periplasmic ligand-binding protein, Dret_0059, from Desulfohalobium retbaense DSM 5692, an organism isolated from Lake Retba, in Senegal. The structure of the protein consists of a unique combination of a periplasmic solute binding protein (SBP) domain at the N-terminal and a tandem PAS-like sensor domain at the C-terminal region. SBP domains are found ubiquitously, and their best known function is in solute transport across membranes. PAS-like sensor domains are commonly found in signal transduction proteins. These domains are widely observed as parts of many protein architectures and complexes but have not been observed previously within the same polypeptide chain. In the structure of Dret_0059, a ketoleucine moiety is bound to the SBP, whereas a cytosine molecule is bound in the distal PAS-like domain of the tandem PAS-like domain. Differential scanning flourimetry support the binding of ligands observed in the crystal structure. There is significant interaction between the SBP and tandem PAS-like domains, and it is possible that the binding of one ligand could have an effect on the binding of the other. We uncovered three other proteins with this structural architecture in the non-redundant sequence data base, and predict that they too bind the same substrates. The genomic context of this protein did not offer any clues for its function. We did not find any biological process in which the two observed ligands are coupled. The protein Dret_0059 could be involved in either signal transduction or solute transport. [Mh] Termos MeSH primário: Proteínas de Bactérias/química Deltaproteobacteria/química Transdução de Sinais [Mh] Termos MeSH secundário: Proteínas de Bactérias/genética Proteínas de Bactérias/metabolismo Deltaproteobacteria/genética Deltaproteobacteria/metabolismo Domínios Proteicos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Bacterial Proteins) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170717 [Lr] Data última revisão: 170717 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170208 [St] Status: MEDLINE [do] DOI: 10.1002/pro.3134

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[PMID]: 28158724 [Au] Autor: Tarpgaard IH; Jørgensen BB; Kjeldsen KU; Røy H [Ti] Título: The marine sulfate reducer Desulfobacterium autotrophicum HRM2 can switch between low and high apparent half-saturation constants for dissimilatory sulfate reduction. [So] Source: FEMS Microbiol Ecol;93(4), 2017 Apr 01. [Is] ISSN: 1574-6941 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Studies of the kinetics of dissimilatory sulfate reduction in marine sediment have shown that a mixture of marine sulfate-reducing bacteria (SRB) can reduce sulfate with both a high and low apparent sulfate half-saturation constant (Km). However, all marine pure cultures investigated have shown only low-sulfate affinity sulfate reduction kinetics. It remains unknown whether marine high sulfate-affinity sulfate reduction is catalyzed by unknown SRB or whether known SRB possess unrecognized high-affinity sulfate reduction systems. We used 35S-sulfate incubation experiments to show that cultures of Desulfobacterium autotrophicum HMR2 will switch from low-affinity to high-affinity sulfate reduction when sulfate concentrations fall below 500 µM. The mean Km was 150 µM at high sulfate concentrations and 8 µM at low sulfate concentrations. The high-affinity Km value is comparable to values found in SRB inhabiting freshwater sediments and D. autotrophicum cultures could deplete sulfate to below our detection limit of 25 nM. The switch in Km value was accompanied by a change in the expression of genes encoding membrane-bound transport proteins putatively involved in sulfate uptake in D. autotrophicum. Our results demonstrate that a marine sulfate reducer can efficiently reduce sulfate at both high and low sulfate concentrations, possibly by activation of different sulfate transporters in the membrane. [Mh] Termos MeSH primário: Deltaproteobacteria/fisiologia Sulfatos/metabolismo [Mh] Termos MeSH secundário: Deltaproteobacteria/genética Desulfovibrio/metabolismo Sedimentos Geológicos/microbiologia Oxirredução Microbiologia da Água [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Sulfates) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170620 [Lr] Data última revisão: 170620 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170204 [St] Status: MEDLINE [do] DOI: 10.1093/femsec/fix012

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