Base de dados : MEDLINE
Pesquisa : B03.660.125.500 [Categoria DeCS]
Referências encontradas : 940 [refinar]
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[PMID]:29458458
[Au] Autor:Mohr KI; Moradi A; Glaeser SP; Kämpfer P; Gemperlein K; Nübel U; Schumann P; Müller R; Wink J
[Ad] Endereço:1​Microbial Drugs, Helmholtz Centre for Infection Research (HZI), 38124 Braunschweig, Germany.
[Ti] Título:Nannocystis konarekensis sp. nov., a novel myxobacterium from an Iranian desert.
[So] Source:Int J Syst Evol Microbiol;68(3):721-729, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An orange-coloured myxobacterium, MNa11734 , was isolated from desert in Iran. MNa11734 had rod-shaped vegetative cells, moved by gliding and was bacteriolytic. No real fruiting body formation could be observed, but sporangioles were produced on water agar. The strain was mesophilic, strictly aerobic and chemoheterotrophic. 16S rRNA gene analyses revealed that MNa11734 belonged to the family Nannocystaceae, genus Nannocystis and was closely related to Nannocystis pusilla Na p29 (DSM 14622 ) and Nannocystis exedens Na e1 (DSM 71 ), with 97.8 and 97.6 % 16S rRNA gene sequence similarity, respectively. Laboratory-measured DNA-DNA hybridization showed only 9.5/15.7 % (reciprocal) similarity between the novel strain and N. pusilla Na p29 , and 14.1/20.4 % between the strain and N. exedens Na e1 , whereas DNA-DNA hybridization estimates derived from draft genome sequences were 21.8-23.0 % and 22.2-23.7 %, respectively, depending on the calculation method. The G+C content of DNA from Nannocystis konarekensis MNa11734 was 73.3 mol%, for N. pusilla Nap29 it was 71.8 mol% and for N. exedens Nae1 it was 72.2 mol%. The major fatty acids of the new strain were C16 : 1 (56.2 %), iso-C17 : 0 (14.4 %), C14 : 0 (8.2 %), C16 : 0 (6.6 %) and iso-C15 : 0 (5.9 %). Strain MNa11734 exhibited phylogenetic and physiological similarities to the two other species of Nannocystis, i.e. N. pusilla and N. exedens, but the differences were sufficient enough to represent a novel species, for which the name Nannocystiskonarekensis sp. nov. is proposed. The type strain is MNa11734 (=DSM 104509 =NCCB 100618 ).
[Mh] Termos MeSH primário: Clima Desértico
Myxococcales/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Irã (Geográfico)
Myxococcales/genética
Myxococcales/isolamento & purificação
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002569


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[PMID]:28467833
[Au] Autor:Bian X; Tang B; Yu Y; Tu Q; Gross F; Wang H; Li A; Fu J; Shen Y; Li YZ; Stewart AF; Zhao G; Ding X; Müller R; Zhang Y
[Ad] Endereço:Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, School of Life Science, Shandong University , Qingdao 266235, China.
[Ti] Título:Heterologous Production and Yield Improvement of Epothilones in Burkholderiales Strain DSM 7029.
[So] Source:ACS Chem Biol;12(7):1805-1812, 2017 Jul 21.
[Is] ISSN:1554-8937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The cloning of microbial natural product biosynthetic gene clusters and their heterologous expression in a suitable host have proven to be a feasible approach to improve the yield of valuable natural products and to begin mining cryptic natural products in microorganisms. Myxobacteria are a prolific source of novel bioactive natural products with only limited choices of heterologous hosts that have been exploited. Here, we describe the use of Burkholderiales strain DSM 7029 as a potential heterologous host for the functional expression of myxobacterial secondary metabolites. Using a newly established electroporation procedure, the 56 kb epothilone biosynthetic gene cluster from the myxobacterium Sorangium cellulosum was introduced into the chromosome of strain DSM 7029 by transposition. Production of epothilones A, B, C, and D was detected despite their yields being low. Optimization of the medium, introduction of the exogenous methylmalonyl-CoA biosynthetic pathway, and overexpression of rare tRNA genes resulted in an approximately 75-fold increase in the total yields of epothilones to 307 µg L . These results show that strain DSM 7029 has the potential to produce epothilones with reasonable titers and might be a broadly applicable host for the heterologous expression of other myxobacterial polyketide synthases and nonribosomal peptide synthetases, expediting the process of genome mining.
[Mh] Termos MeSH primário: Produtos Biológicos/metabolismo
Epotilonas/biossíntese
Microbiologia Industrial/métodos
Myxococcales/metabolismo
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Eletroporação
Epotilonas/química
Epotilonas/genética
Estrutura Molecular
Myxococcales/genética
RNA de Transferência/genética
RNA de Transferência/metabolismo
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Epothilones); 9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1021/acschembio.7b00097


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[PMID]:28841794
[Au] Autor:Lee H; Park Y; Kim S
[Ad] Endereço:Department of Chemistry, Seoul National University , Seoul 08826, Republic of Korea.
[Ti] Título:Enzymatic Cross-Linking of Side Chains Generates a Modified Peptide with Four Hairpin-like Bicyclic Repeats.
[So] Source:Biochemistry;56(37):4927-4930, 2017 Sep 19.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Macrocyclization of peptides is often employed to generate novel structures and biological activities in the biosynthesis of natural products and drug discovery. The enzymatic cross-linking of two side chains in a peptide via an ester or amide has a high potential for making topologically diverse cyclic peptides but is found with only a single consensus sequence in the microviridin class of natural products. Here, we report that a peptide with a new sequence pattern can be enzymatically cross-linked to make a novel microviridin-like peptide, plesiocin, which contains four repeats of a distinct hairpin-like bicyclic structure and shows strong inhibition of proteases. A single ATP-grasp enzyme binds to a leader peptide, of which only 13 residues are required for binding, and performs eight esterification reactions on the core peptide. We also demonstrate that the combination of tandem mass spectrometry and an ester-specific reaction greatly facilitates the determination of connectivity. We suggest that the enzymatic cross-linking of peptide side chains can generate more diverse structures in nature or by engineering.
[Mh] Termos MeSH primário: Organismos Aquáticos/metabolismo
Desenho de Drogas
Myxococcales/metabolismo
Peptídeos Cíclicos/metabolismo
Peptídeos/metabolismo
Inibidores de Proteases/metabolismo
Processamento de Proteína Pós-Traducional
[Mh] Termos MeSH secundário: Organismos Aquáticos/enzimologia
Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Proteínas de Bactérias/farmacologia
Cromatografia Líquida de Alta Pressão
Quimotripsina/antagonistas & inibidores
Quimotripsina/metabolismo
Esterificação
Interações Hidrofóbicas e Hidrofílicas
Sequências Repetidas Invertidas
Cinética
Estrutura Molecular
Família Multigênica
Myxococcales/enzimologia
Elastase Pancreática/antagonistas & inibidores
Elastase Pancreática/metabolismo
Peptídeos/química
Peptídeos/farmacologia
Peptídeos Cíclicos/química
Peptídeos Cíclicos/farmacologia
Inibidores de Proteases/química
Inibidores de Proteases/farmacologia
Conformação Proteica
Proteólise/efeitos dos fármacos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Peptides); 0 (Peptides, Cyclic); 0 (Protease Inhibitors); 0 (plesiocin); EC 3.4.21.1 (Chymotrypsin); EC 3.4.21.36 (Pancreatic Elastase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170827
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00808


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[PMID]:28796488
[Au] Autor:Filipuzzi I; Thomas JR; Pries V; Estoppey D; Salcius M; Studer C; Schirle M; Hoepfner D
[Ad] Endereço:Novartis Institutes for BioMedical Research , Novartis Pharma AG, Forum 1 Novartis Campus, CH-4056 Basel, Switzerland.
[Ti] Título:Direct Interaction of Chivosazole F with Actin Elicits Cell Responses Similar to Latrunculin A but Distinct from Chondramide.
[So] Source:ACS Chem Biol;12(9):2264-2269, 2017 Sep 15.
[Is] ISSN:1554-8937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The microbial metabolite Chivosazole F has been described to affect the cytoskeleton and to inhibit actin polymerization in vitro. Applying orthogonal genomic and proteomics approaches, we now show for the first time that Chivosazole F exerts its effect by directly interacting with actin and demonstrate the cellular impact of Chivosazole F in an unbiased, genome-wide context in yeast and in mammalian cells. Furthermore, mutation-based resistance mapping identifies two SNPs located in the putative Chivosazole F binding site of actin. Comparing chemogenomic profiles and responses to the Chivosazole F-resistant SNPs shows a partially conserved mechanism of action for Chivosazole F and Latrunculin A, but clear divergence from Chondramide. In addition, C14orf80 is an evolutionarily highly conserved ORF, lacking any functional annotation. As editing of C14orf80 leads to Chivosazole F hyper-resistance, we propose a function for this gene product in counteracting perturbation of actin filaments.
[Mh] Termos MeSH primário: Actinas/metabolismo
Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia
Depsipeptídeos/farmacologia
Macrolídeos/farmacologia
Tiazolidinas/farmacologia
[Mh] Termos MeSH secundário: Citoesqueleto de Actina/efeitos dos fármacos
Citoesqueleto de Actina/genética
Citoesqueleto de Actina/metabolismo
Actinas/genética
Sítios de Ligação
Compostos Bicíclicos Heterocíclicos com Pontes/química
Depsipeptídeos/química
Células HEK293
Seres Humanos
Macrolídeos/química
Mutação
Myxococcales/química
Tiazolidinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Bridged Bicyclo Compounds, Heterocyclic); 0 (Depsipeptides); 0 (Macrolides); 0 (Thiazolidines); 0 (chivosazole F); 0 (chondramide A); SRQ9WWM084 (latrunculin A)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1021/acschembio.7b00385


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[PMID]:28636395
[Au] Autor:Muddala R; Acosta JAM; Barbosa LCA; Boukouvalas J
[Ad] Endereço:Department of Chemistry and Centre for Green Chemistry and Catalysis, Université Laval , 1045 Avenue de la Médecine, Quebec City, Quebec G1V 0A6, Canada.
[Ti] Título:Synthesis of the Marine Myxobacterial Antibiotic Enhygrolide A.
[So] Source:J Nat Prod;80(7):2166-2169, 2017 Jul 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The first synthesis of enhygrolide A, a scarce γ-alkylidenebutenolide antibiotic of the obligate marine myxobacterium Enhygromyxa salina, was achieved in five steps and 54% overall yield from tetronic acid. Key steps include (i) organocatalytic reductive alkylation, (ii) iron-catalyzed sp -sp cross-coupling, and (iii) vinylogous aldol condensation. Aside from its brevity and reliance on environmentally sustainable processes, the synthesis demonstrates the serviceability of butenolide pivalates in cross-coupling reactions.
[Mh] Termos MeSH primário: 4-Butirolactona/análogos & derivados
Antibacterianos/síntese química
Compostos de Benzilideno/síntese química
Myxococcales/química
[Mh] Termos MeSH secundário: 4-Butirolactona/síntese química
4-Butirolactona/química
4-Butirolactona/farmacologia
Aldeídos/química
Alquilação
Antibacterianos/química
Antibacterianos/farmacologia
Compostos de Benzilideno/química
Compostos de Benzilideno/farmacologia
Catálise
Biologia Marinha
Estrutura Molecular
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aldehydes); 0 (Anti-Bacterial Agents); 0 (Benzylidene Compounds); 0 (enhygrolide A); 8C6G962B53 (3-hydroxybutanal); 8KXK25H388 (butenolide); OL659KIY4X (4-Butyrolactone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.7b00405


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[PMID]:28383484
[Au] Autor:Tomura T; Nagashima S; Yamazaki S; Iizuka T; Fudou R; Ojika M
[Ad] Endereço:Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan. ttomura@agr.nagoya-u.ac.jp.
[Ti] Título:An Unusual Diterpene-Enhygromic Acid and Deoxyenhygrolides from a Marine Myxobacterium, Enhygromyxa sp.
[So] Source:Mar Drugs;15(4), 2017 Apr 06.
[Is] ISSN:1660-3397
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Three new compounds, enhygromic acid ( ) and deoxyenhygrolides A ( ) and B ( ), were isolated from a marine myxobacterium, sp. Compound was found to be an acrylic acid derivative with a rare polycyclic carbon skeleton, decahydroacenaphthylene, by spectroscopic analyses. Compounds and were deoxy analogs of the known γ-alkylidenebutenolides, enhygrolides. Compound exhibited cytotoxicity against B16 melanoma cells and anti-bacterial activity against , and enhanced the NGF-induced neurite outgrowth of PC12 cells.
[Mh] Termos MeSH primário: Organismos Aquáticos/química
Diterpenos/química
Myxococcales/química
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Antibacterianos/farmacologia
Bacillus subtilis/efeitos dos fármacos
Linhagem Celular Tumoral
Diterpenos/farmacologia
Neuritos/química
Células PC12
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Diterpenes)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE


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[PMID]:28281299
[Au] Autor:Khatri Y; Schifrin A; Bernhardt R
[Ad] Endereço:Institute of Biochemistry, Saarland University, Saarbrücken, Germany.
[Ti] Título:Investigating the effect of available redox protein ratios for the conversion of a steroid by a myxobacterial CYP260A1.
[So] Source:FEBS Lett;591(8):1126-1140, 2017 Apr.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Since cytochromes P450 are external monooxygenases, available surrogate redox partners have been used to reconstitute the P450 activity. However, the effect of various ratios of P450s and the redox proteins have not been extensively studied so far, although different combinations of the redox partners have shown variations in substrate conversion. To address this issue, CYP260A1 was reconstituted with various ratios of adrenodoxin and adrenodoxin reductase to convert 11-deoxycorticosterone, and the products were characterized by NMR. We show the effect of the available redox protein ratios not only on the P450 catalytic activity but also on the product pattern.
[Mh] Termos MeSH primário: Adrenodoxina/metabolismo
Proteínas de Bactérias/metabolismo
Desoxicorticosterona/metabolismo
Ferredoxina-NADP Redutase/metabolismo
Modelos Moleculares
Myxococcales/enzimologia
Ácido Retinoico 4 Hidroxilase/metabolismo
Esteroide Hidroxilases/metabolismo
[Mh] Termos MeSH secundário: Adrenodoxina/genética
Animais
Ácido Ascórbico/metabolismo
Proteínas de Bactérias/genética
Biocatálise
Catalase/metabolismo
Desoxicorticosterona/análogos & derivados
Desoxicorticosterona/química
Ferredoxina-NADP Redutase/genética
Depuradores de Radicais Livres/metabolismo
Peróxido de Hidrogênio/química
Espectroscopia de Ressonância Magnética
Estrutura Molecular
NADP/metabolismo
Oxirredução
Proteínas Recombinantes/metabolismo
Estereoisomerismo
Esteroide Hidroxilases/genética
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (11-deoxycorticosterone-1-ene); 0 (Bacterial Proteins); 0 (Free Radical Scavengers); 0 (Recombinant Proteins); 12687-22-8 (Adrenodoxin); 40GP35YQ49 (Desoxycorticosterone); 53-59-8 (NADP); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.6 (Catalase); EC 1.14.- (Steroid Hydroxylases); EC 1.14.14.1 (Retinoic Acid 4-Hydroxylase); EC 1.15.1.1 (Superoxide Dismutase); EC 1.18.1.2 (Ferredoxin-NADP Reductase); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12619


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[PMID]:28217903
[Au] Autor:Arias Del Angel JA; Escalante AE; Martínez-Castilla LP; Benítez M
[Ad] Endereço:Laboratorio Nacional de Ciencias de la Sostenibilidad (LANCIS), Instituto de Ecologiía, Universidad Nacional Autónoma de México, Mexico City, Mexico.
[Ti] Título:An Evo-Devo Perspective on Multicellular Development of Myxobacteria.
[So] Source:J Exp Zool B Mol Dev Evol;328(1-2):165-178, 2017 Jan.
[Is] ISSN:1552-5015
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The transition to multicellularity, recognized as one the major transitions in evolution, has occurred independently several times. While multicellular development has been extensively studied in zygotic organisms including plant and animal groups, just a few aggregative multicellular organisms have been employed as model organisms for the study of multicellularity. Studying different evolutionary origins and modes of multicellularity enables comparative analyses that can help identifying lineage-specific aspects of multicellular evolution and generic factors and mechanisms involved in the transition to multicellularity. Among aggregative multicellular organisms, myxobacteria are a valuable system to explore the particularities that aggregation confers to the evolution of multicellularity and mechanisms shared with clonal organisms. Moreover, myxobacteria species develop fruiting bodies displaying a range of morphological diversity. In this review, we aim to synthesize diverse lines of evidence regarding myxobacteria development and discuss them in the context of Evo-Devo concepts and approaches. First, we briefly describe the developmental processes in myxobacteria, present an updated comparative analysis of the genes involved in their developmental processes and discuss these and other lines of evidence in terms of co-option and developmental system drift, two concepts key to Evo-Devo studies. Next, as has been suggested from Evo-Devo approaches, we discuss how broad comparative studies and integration of diverse genetic, physicochemical, and environmental factors into experimental and theoretical models can further our understanding of myxobacterial development, phenotypic variation, and evolution.
[Mh] Termos MeSH primário: Evolução Biológica
Biologia do Desenvolvimento
Regulação Bacteriana da Expressão Gênica/fisiologia
Myxococcales/citologia
Myxococcales/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE
[do] DOI:10.1002/jez.b.22727


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[PMID]:28168528
[Au] Autor:Young B; Delatolla R; Abujamel T; Kennedy K; Laflamme E; Stintzi A
[Ad] Endereço:Department of Civil Engineering, Faculty of Engineering, University of Ottawa, 161, Louis Pasteur, Ottawa, ON, K1N 6N5, Canada.
[Ti] Título:Rapid start-up of nitrifying MBBRs at low temperatures: nitrification, biofilm response and microbiome analysis.
[So] Source:Bioprocess Biosyst Eng;40(5):731-739, 2017 May.
[Is] ISSN:1615-7605
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The moving bed biofilm reactor (MBBR), operated as a post carbon removal system, requires long start-up times in comparison to carbon removal systems due to slow growing autotrophic organisms. This study investigates the use of carriers seeded in a carbon rich treatment system prior to inoculation in a nitrifying MBBR system to promote the rapid development of nitrifying biofilm in an MBBR system at temperatures between 6 and 8 °C. Results show that nitrification was initiated by the carbon removal carriers after 22 h of operation. High throughput 16S-rDNA sequencing indicates that the sloughing period was a result of heterotrophic organism detachment and the recovery and stabilization period included a growth of Nitrosomonas and Nitrospira as the dominant ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) in the biofilm. Peripheral microorganisms such as Myxococcales, a rapid EPS producer, appear to have contributed to the recovery and stabilization of the biofilm.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Reatores Biológicos/microbiologia
Microbiota/fisiologia
Myxococcales/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1007/s00449-017-1739-5


  10 / 940 MEDLINE  
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[PMID]:28161814
[Au] Autor:Charousová I; Steinmetz H; Medo J; Javoreková S; Wink J
[Ad] Endereço:Faculty of Biotechnology and Food Sciences, Department of Microbiology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76, Nitra, Slovak Republic. ivanacharousova@gmail.com.
[Ti] Título:Soil myxobacteria as a potential source of polyketide-peptide substances.
[So] Source:Folia Microbiol (Praha);62(4):305-315, 2017 Jul.
[Is] ISSN:1874-9356
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Myxobacteria, a group of antimicrobial producing bacteria, have been successfully cultured and characterized from ten soil samples collected from different parts of Slovakia. A total of 79 myxobacteria belonging to four genera (Myxococcus, Corallococcus, Sorangium, and Polyangium) were isolated based on aspects of their life cycle. Twenty-five of them were purified, fermented, and screened for antimicrobial activities against 11 test microorganisms. Results indicated that crude extracts showed more significant activities against Gram-positive than against Gram-negative bacteria or fungi. Based on a higher degree and broader range of antimicrobial production, the two most potential extracts (K9-5, V3-1) were selected for HPLC fractionation against Micrococcus luteus and Staphylococcus aureus and LC/MS analysis of potential antibiotic metabolites. The analysis resulted in the identification of polyketide-peptide antibiotics, namely corallopyronin A and B (K9-5) and myxalamid B and C (V3-1), which were responsible for important Gram-positive activity in the observed strains. A sequence similarity search through BLAST revealed that these strains showed the highest sequence similarity to Corallococcus coralloides (K9-5, NCBI accession number KX256198) and Myxococcus xanthus (V3-1, NCBI accession number KX256197). Although screening of myxobacteria is laborious, due to difficulties in isolating cultures, this research represented the first report covering the isolation and cultivation of this challenging bacterial group from Slovakian soils as well as the screening of their antimicrobial activity, cultural identification, and secondary metabolite identification.
[Mh] Termos MeSH primário: Antibacterianos/metabolismo
Myxococcales/química
Policetídeos/metabolismo
Microbiologia do Solo
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/farmacologia
Micrococcus luteus/efeitos dos fármacos
Myxococcales/genética
Myxococcales/isolamento & purificação
Myxococcales/metabolismo
Filogenia
Policetídeos/química
Policetídeos/farmacologia
Staphylococcus aureus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Polyketides)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170206
[St] Status:MEDLINE
[do] DOI:10.1007/s12223-017-0502-2



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