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[PMID]:29458490
[Au] Autor:Shi MJ; Wang C; Wang XT; Du ZJ
[Ad] Endereço:1​State key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
[Ti] Título:Halioglobus lutimaris sp. nov., isolated from coastal sediment.
[So] Source:Int J Syst Evol Microbiol;68(3):876-880, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel Gram-stain-negative, strictly aerobic, non-flagellated and rod-shaped bacterium, designated HF004 , was isolated from a marine sediment sample collected from the coast of Weihai, China. The strain grew optimally at 28 °C, pH 7.5-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Based on the 16S rRNA gene sequence analysis, strain HF004 was a member of the genus Halioglobus, appearing to be closely related to Halioglobus pacificus (96.1 %) and Halioglobus japonicus (95.6 %). The major fatty acids were summed feature 3 (i.e. C16 : 1ω7c and/or iso-C15 : 0 2-OH), C17 : 1ω8c and C18 : 1ω7c. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. The DNA G+C content was 57.2 mol%. Cells of strain HF004 were rod-shaped and formed circular, mucous and beige-pigmented colonies on marine agar after incubation for 72 h at 28 °C. On the basis of phenotypic, genotypic and phylogenetic evidence, strain HF004 is presented as a novel species, for which the name Halioglobus lutimaris sp. nov. is proposed. The type strain is HF004 (=KCTC 42395 =MCCC 1H00127 ).
[Mh] Termos MeSH primário: Gammaproteobacteria/classificação
Sedimentos Geológicos/microbiologia
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Gammaproteobacteria/genética
Gammaproteobacteria/isolamento & purificação
Fosfatidilgliceróis/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phosphatidylglycerols); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002601


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[PMID]:29388544
[Au] Autor:Christiansen L; Bech PK; Schultz-Johansen M; Martens HJ; Stougaard P
[Ad] Endereço:Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
[Ti] Título:Colwellia echini sp. nov., an agar- and carrageenan-solubilizing bacterium isolated from sea urchin.
[So] Source:Int J Syst Evol Microbiol;68(2):687-691, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel bacterial strain, A3 , was isolated from the intestines of the sea urchin Strongylocentrotus droebachiensis collected in Øresund, Denmark. The strain was Gram-reaction-negative, rod-shaped and facultatively anaerobic, and displayed growth at 5-25 °C (optimum 20 °C), pH 7-9 (optimum at pH 7) and 1-6 % (w/v) NaCl (optimum 3 %). Furthermore, strain A3 grew on agar, agarose, κ-carrageenan, alginate and laminarin as sole carbon source. Complete liquefaction of agar and κ-carrageenan was observed on solid plate media as a result of enzymatic activities. Major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The respiratory quinones were determined to be ubiquinones Q-8 (92 %) and Q-7 (8 %), and polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 36.9 mol%. Phylogenetical analyses based on the 16S rRNA gene showed that the bacterium was affiliated with the genus Colwellia within the Alteromonadaceae of the Gammaproteobacteria. The level of 16S rRNA gene sequence similarity between strain A3 and its closest relatives in the genus Colwellia (C. psychrerythraea ATCC 27364 and C. asteriadis KMD 002 ) was 97.5 %. The average nucleotide identity between strain A3 and other members of Colwellia was 78.6-80.5 %, and DNA-DNA hybridization prediction revealed values of less than 23 % relatedness between strain A3 and other Colwellia species. The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain A3 represents a novel species of the genus Colwellia, for which the name Colwellia echini sp. nov. is proposed. The type strain is A3 (=LMG 30125 =NCIMB 15095 ).
[Mh] Termos MeSH primário: Alteromonadaceae/classificação
Filogenia
Strongylocentrotus/microbiologia
[Mh] Termos MeSH secundário: Ágar
Alginatos
Alteromonadaceae/genética
Alteromonadaceae/isolamento & purificação
Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
Carragenina
DNA Bacteriano/genética
Dinamarca
Ácidos Graxos/química
Gammaproteobacteria
Glucanos
Ácido Glucurônico
Ácidos Hexurônicos
Fosfatidiletanolaminas/química
Fosfatidilgliceróis/química
RNA Ribossômico 16S/genética
Sefarose
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Glucans); 0 (Hexuronic Acids); 0 (Phosphatidylethanolamines); 0 (Phosphatidylglycerols); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); 39382-08-6 (phosphatidylethanolamine); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); 9000-07-1 (Carrageenan); 9002-18-0 (Agar); 9008-22-4 (laminaran); 9012-36-6 (Sepharose); CQA993F7P8 (ubiquinone 8); RRK47DEG6Q (ubiquinone 7)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002568


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[PMID]:29185937
[Au] Autor:Xiao M; Salam N; Liu L; Jiao JY; Zheng ML; Wang J; Li S; Chen C; Li WJ; Qu PH
[Ad] Endereço:1​State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.
[Ti] Título:Fastidiosibacter lacustris gen. nov., sp. nov., isolated from a lake water sample, and proposal of Fastidiosibacteraceae fam. nov. within the order Thiotrichales.
[So] Source:Int J Syst Evol Microbiol;68(1):347-352, 2018 Jan.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AFrancisella-like bacterium, designated strain SYSU HZH-2 , was isolated from a water sample collected from Haizhu Lake, Guangzhou, China. The bacterium was fastidious, and required an exogenous source of l-cysteine for its growth on artificial media. Cells were Gram-stain-negative, coccobacilli, non-motile and non-spore-forming. The strain shared highest 16S rRNA gene sequence similarities with Cysteiniphilum litorale SYSU D3-2 (94.6 % identity), Fangia hongkongensis UST040201-002 (93.2 %) and Caedibacter taeniospiralis 51 (91.6 %). This strain possessed ubiquinone-8 as the respiratory quinone; diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol as the known polar lipids, and anteiso-C15 : 0 and C18 : 0 as the major fatty acids (>10 % of total fatty acids). The dendrograms based on 16S rRNA gene sequence analysis showed that it formed a separate cluster along with Cysteiniphilum litorale SYSU D3-2 , Caedibactertaeiniospiralis 51 and Fangia hongkongensis UST040201-002 . Based on the 16S rRNA gene sequence identity and differences in other phenotypic characteristics, the strain is considered to represent a novel species of a novel genus, for which the name Fastidiosibacter lacustris gen. nov., sp. nov. is proposed. The type strain of the type species Fastidiosibacter lacustris is SYSU HZH-2 (=NBRC 112274 = CGMCC 1.15950 ). Additionally, the new taxon along with the genera Caedibacter, Cysteiniphilum and Fangia (family unassigned) were distinctly separated from the related families Francisellaceae, Piscirickettsiaceae and Thiotrichaeae in the phylogenetic trees. Therefore, we proposed a new family Fastidiosibacteraceae fam. nov. within the order Thiotrichales to accommodate these four genera.
[Mh] Termos MeSH primário: Gammaproteobacteria/classificação
Lagos/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Gammaproteobacteria/genética
Gammaproteobacteria/isolamento & purificação
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002510


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[PMID]:29364604
[Au] Autor:Galach'yants AD; Bel'kova NL; Sukhanova EV; Romanovskaya VA; Gladka GV; Bedoshvili ED; Parfenova VV
[Ti] Título:[Diversity and Physiological and Biochemical Properties of Heterotrophic Bacteria. Isolated from Lake Baikal Neuston.]
[So] Source:Mikrobiologiia;85(5):568-579, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:For heterotrophic microorganisms (44 strains) isolated-from the surface film of Lake Baikal, iden- tification was carried out and their. physiological and biochemical characteristics were determined. Com- pared to the water column, diversity of cultured heterotrophs was low, indicating formation of stable micro- bial communities at the air-water interphase interface. Heterotrophic bacteria isolated from the surface mi- crolayer exhibited the enzymatic activity comparable to that for strains form other biofilm associations. Deinococcusfi6us strain NA202 'vas the most active component of the community, capable of utilization of the broadest spectrum of mono- and disaccharides,'sugars, and amino acids. This strain possessed the highest diversity of extracellular enzymes and was the most resistant to UV radiation. The physiological and bio- chemical properties of this strain may-be responsible for its adaptation to survival in extreme conditions of the surface microlayer. Our results improve our understanding of occurrence of UV-resistant strains in freshwater ecosystems.
[Mh] Termos MeSH primário: Deinococcus/metabolismo
Processos Heterotróficos/fisiologia
Lagos/microbiologia
Consórcios Microbianos/fisiologia
Filogenia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Alphaproteobacteria/classificação
Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Alphaproteobacteria/efeitos da radiação
Aminoácidos/metabolismo
Bacteroidetes/classificação
Bacteroidetes/genética
Bacteroidetes/isolamento & purificação
Bacteroidetes/efeitos da radiação
Betaproteobacteria/classificação
Betaproteobacteria/genética
Betaproteobacteria/isolamento & purificação
Betaproteobacteria/efeitos da radiação
Biodiversidade
Deinococcus/classificação
Deinococcus/isolamento & purificação
Deinococcus/efeitos da radiação
Deltaproteobacteria/classificação
Deltaproteobacteria/genética
Deltaproteobacteria/isolamento & purificação
Deltaproteobacteria/efeitos da radiação
Dissacarídeos/metabolismo
Ecossistema
Firmicutes/classificação
Firmicutes/genética
Firmicutes/isolamento & purificação
Firmicutes/efeitos da radiação
Gammaproteobacteria/classificação
Gammaproteobacteria/genética
Gammaproteobacteria/isolamento & purificação
Gammaproteobacteria/efeitos da radiação
Processos Heterotróficos/efeitos da radiação
Consórcios Microbianos/efeitos da radiação
Monossacarídeos/metabolismo
Proteobactérias/classificação
Proteobactérias/genética
Proteobactérias/isolamento & purificação
Proteobactérias/efeitos da radiação
Sibéria
Propriedades de Superfície
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Disaccharides); 0 (Monosaccharides); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


  5 / 1732 MEDLINE  
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[PMID]:29364602
[Au] Autor:Danilova OV; Belova SE; Gagarinova IV; Dedysh SN
[Ti] Título:Microbial Community Composition and Methanotroph Diversity of a Subarctic Wetland in Russia.
[So] Source:Mikrobiologiia;85(5):545-554, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:This study assessed the microbial diversity, activity, and composition of methane-oxidizing communities of a subarctic wetland in Russia,with mosaic cover of Sphagnum mosses and lichens of the genera Cladonia and Cetraria. Potential methane-oxidizing activity of peat sampled from lichen-dominated wetland sites was higher than that in the sites dominated by Sphagnum mosses. In peat from lichendominated sites, major bacterial groups identified by high-throughput sequencing of the 16S rRNA genes were the Acidobacteria (35.4-41.2% of total 16S rRNA gene reads), Alphaproteobacteria (19.1-24.2%), Gammaproteobacteria (7.9-11.1%), Actinobacteria (5.5-13.2%), Planctomycetes (7.2-9.5%), and Verrucomicrobia (5.1-9.5%). The distinctive feature of this community was high proportion of Subdivision 2 Acidobacteria, which are not char- acteristic for boreal Sphagnum peat bogs. Methanotrophic community composition was determined by mo- lecular analysis of the pmoA gene encoding particulate methane monooxygenase. Most (-80%) of all pmoA gene fragments revealed in peat from lichen-dominated sites belonged to the phylogenetic lineage represented by a microaerobic spiral-shaped methanotroph, "Candidatus Methylospira mobilis." Members of the genus Methylocystis, which are typical inhabitants of boreal Sphagnum peat bogs, represented only a minor group of indigenous methanotrophs. The specific feature of a methanotrophic community in peat from lichen-dominated sites was the presence of uncultivated USCa (Upland Soil Cluster alpha) methanotrophs, which are typical for acidic upland soils showing atmospheric methane oxidation. The methanotrophic community composition in lichen-dominated sites of a tundra wetland, therefore, was markedly different from that in bo- real Sphagnum peat bogs.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Água Subterrânea/microbiologia
Metano/metabolismo
Consórcios Microbianos/fisiologia
Oxigenases/genética
[Mh] Termos MeSH secundário: Acidobacteria/classificação
Acidobacteria/genética
Acidobacteria/isolamento & purificação
Acidobacteria/metabolismo
Actinobacteria/classificação
Actinobacteria/genética
Actinobacteria/isolamento & purificação
Actinobacteria/metabolismo
Alphaproteobacteria/classificação
Alphaproteobacteria/genética
Alphaproteobacteria/isolamento & purificação
Alphaproteobacteria/metabolismo
Regiões Árticas
Proteínas de Bactérias/metabolismo
Briófitas/fisiologia
Gammaproteobacteria/classificação
Gammaproteobacteria/genética
Gammaproteobacteria/isolamento & purificação
Gammaproteobacteria/metabolismo
Expressão Gênica
Líquens/fisiologia
Metano/química
Oxigenases/metabolismo
Filogenia
Planctomycetales/classificação
Planctomycetales/genética
Planctomycetales/isolamento & purificação
Planctomycetales/metabolismo
Federação Russa
Verrucomicrobia/classificação
Verrucomicrobia/genética
Verrucomicrobia/isolamento & purificação
Verrucomicrobia/metabolismo
Zonas Úmidas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 1.13.- (Oxygenases); EC 1.14.13.25 (methane monooxygenase); OP0UW79H66 (Methane)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:27775698
[Au] Autor:König S; Gros O; Heiden SE; Hinzke T; Thürmer A; Poehlein A; Meyer S; Vatin M; Mbéguié-A-Mbéguié D; Tocny J; Ponnudurai R; Daniel R; Becher D; Schweder T; Markert S
[Ad] Endereço:Department of Pharmaceutical Biotechnology, E.M.A. University of Greifswald, Institute of Pharmacy, Greifswald, Germany.
[Ti] Título:Nitrogen fixation in a chemoautotrophic lucinid symbiosis.
[So] Source:Nat Microbiol;2:16193, 2016 Oct 24.
[Is] ISSN:2058-5276
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The shallow water bivalve Codakia orbicularis lives in symbiotic association with a sulfur-oxidizing bacterium in its gills. The endosymbiont fixes CO and thus generates organic carbon compounds, which support the host's growth. To investigate the uncultured symbiont's metabolism and symbiont-host interactions in detail we conducted a proteogenomic analysis of purified bacteria. Unexpectedly, our results reveal a hitherto completely unrecognized feature of the C. orbicularis symbiont's physiology: the symbiont's genome encodes all proteins necessary for biological nitrogen fixation (diazotrophy). Expression of the respective genes under standard ambient conditions was confirmed by proteomics. Nitrogenase activity in the symbiont was also verified by enzyme activity assays. Phylogenetic analysis of the bacterial nitrogenase reductase NifH revealed the symbiont's close relationship to free-living nitrogen-fixing Proteobacteria from the seagrass sediment. The C. orbicularis symbiont, here tentatively named 'Candidatus Thiodiazotropha endolucinida', may thus not only sustain the bivalve's carbon demands. C. orbicularis may also benefit from a steady supply of fixed nitrogen from its symbiont-a scenario that is unprecedented in comparable chemoautotrophic symbioses.
[Mh] Termos MeSH primário: Bivalves/microbiologia
Crescimento Quimioautotrófico
Gammaproteobacteria/fisiologia
Fixação de Nitrogênio
Simbiose
[Mh] Termos MeSH secundário: Animais
Gammaproteobacteria/química
Gammaproteobacteria/genética
Gammaproteobacteria/metabolismo
Brânquias/microbiologia
Redes e Vias Metabólicas/genética
Nitrogenase/metabolismo
Oxirredutases/genética
Filogenia
Proteoma/análise
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proteome); EC 1.- (Oxidoreductases); EC 1.18.6.1 (Nitrogenase); EC 1.18.6.1 (nitrogenase reductase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1038/nmicrobiol.2016.193


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[PMID]:28464532
[Au] Autor:Sogin EM; Putnam HM; Nelson CE; Anderson P; Gates RD
[Ad] Endereço:Hawai'i Institute of Marine Biology, University of Hawai'i at Manoa, Kane'ohe, HI, USA.
[Ti] Título:Correspondence of coral holobiont metabolome with symbiotic bacteria, archaea and Symbiodinium communities.
[So] Source:Environ Microbiol Rep;9(3):310-315, 2017 Jun.
[Is] ISSN:1758-2229
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microbial symbiotic partners, such as those associated with Scleractinian corals, mediate biochemical transformations that influence host performance and survival. While evidence suggests microbial community composition partly accounts for differences in coral physiology, how these symbionts affect metabolic pathways remains underexplored. We aimed to assess functional implications of variation among coral-associated microbial partners in hospite. To this end, we characterized and compared metabolomic profiles and microbial community composition from nine reef-building coral species. These data demonstrate metabolite profiles and microbial communities are species-specific and are correlated to one another. Using Porites spp. as a case study, we present evidence that the relative abundance of different sub-clades of Symbiodinium and bacterial/archaeal families are linked to positive and negative metabolomic signatures. Our data suggest that while some microbial partners benefit the union, others are more opportunistic with potential detriment to the host. Consequently, coral partner choice likely influences cellular metabolic activities and, therefore, holobiont nutrition.
[Mh] Termos MeSH primário: Alveolados/crescimento & desenvolvimento
Antozoários/microbiologia
Antozoários/parasitologia
Archaea/crescimento & desenvolvimento
Gammaproteobacteria/crescimento & desenvolvimento
Simbiose/fisiologia
Vibrionaceae/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Alveolados/metabolismo
Animais
Archaea/classificação
Archaea/metabolismo
Biodiversidade
Recifes de Corais
Gammaproteobacteria/metabolismo
Metaboloma/fisiologia
Vibrionaceae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1111/1758-2229.12541


  8 / 1732 MEDLINE  
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[PMID]:29223156
[Au] Autor:But SY; Egorova SV; Khmelenina VN; Trotsenko YA
[Ad] Endereço:Skryabin Institute of Biochemistry and Physiology of Microorganisms, Laboratory of Methylotrophy, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. flash20063@rambler.ru.
[Ti] Título:Biochemical Properties and Phylogeny of Hydroxypyruvate Reductases from Methanotrophic Bacteria with Different C -Assimilation Pathways.
[So] Source:Biochemistry (Mosc);82(11):1295-1303, 2017 Nov.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the aerobic methanotrophic bacteria Methylomicrobium alcaliphilum 20Z, Methylococcus capsulatus Bath, and Methylosinus trichosporium OB3b, the biochemical properties of hydroxypyruvate reductase (Hpr), an indicator enzyme of the serine pathway for assimilation of reduced C -compounds, were comparatively analyzed. The recombinant Hpr obtained by cloning and heterologous expression of the hpr gene in Escherichia coli catalyzed NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but did not catalyze the reverse reactions of D-glycerate or glycolate oxidation. The absence of the glycerate dehydrogenase activity in the methanotrophic Hpr confirmed a key role of the enzyme in utilization of C -compounds via the serine cycle. The enzyme from Ms. trichosporium OB3b realizing the serine cycle as a sole assimilation pathway had much higher special activity and affinity in comparison to Hpr from Mm. alcaliphilum 20Z and Mc. capsulatus Bath assimilating carbon predominantly via the ribulose monophosphate (RuMP) cycle. The hpr gene was found as part of gene clusters coding the serine cycle enzymes in all sequenced methanotrophic genomes except the representatives of the Verrucomicrobia phylum. Phylogenetic analyses revealed two types of Hpr: (i) Hpr of methanotrophs belonging to the Gammaproteobacteria class, which use the serine cycle along with the RuMP cycle, as well as of non-methylotrophic bacteria belonging to the Alphaproteobacteria class; (ii) Hpr of methylotrophs from Alpha- and Betaproteobacteria classes that use only the serine cycle and of non-methylotrophic representatives of Betaproteobacteria. The putative role and origin of hydroxypyruvate reductase in methanotrophs are discussed.
[Mh] Termos MeSH primário: Hidroxipiruvato Redutase/classificação
Methylococcaceae/enzimologia
Methylosinus/enzimologia
Filogenia
[Mh] Termos MeSH secundário: Alphaproteobacteria
Gammaproteobacteria
Bactérias Aeróbias Gram-Negativas/classificação
Bactérias Aeróbias Gram-Negativas/enzimologia
Hidroxipiruvato Redutase/metabolismo
Methylobacillus
Methylobacteriaceae
Methylophilaceae
Serina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
452VLY9402 (Serine); EC 1.1.1.81 (Hydroxypyruvate Reductase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917110074


  9 / 1732 MEDLINE  
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[PMID]:28901895
[Au] Autor:Huang Z; Lai Q; Zhang D; Shao Z
[Ad] Endereço:1​School of Marine Sciences, Ningbo University, Ningbo 315211, PR China.
[Ti] Título:Agaribacterium haliotis gen. nov., sp. nov., isolated from abalone faeces.
[So] Source:Int J Syst Evol Microbiol;67(10):3819-3823, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A marine bacterium, named strain feces2 , was isolated from the excreted faeces of an abalone, Haliotis discus hannai. The bacterium was Gram-stain-negative, rod-shaped and had a polar flagellum. It formed a white, small and crater-like colony on an agar plate, and had the capability of degrading agar. Activity of oxidase was positive and that of catalase was negative. Strain feces2 grew at 16 to 40 °C with an optimum of 28-30 °C. The nearly full-length 16S rRNA gene of strain feces2 had the greatest sequence similarity of 92.9 % with Marinibactrumhalimedae Q-192 , followed by of 92.8 % with Teredinibacterturnerae T7902 . Phylogenetic analysis indicated that strain feces2 belonged to the family Cellvibrionaceae, representing an independent clade with an uncultured bacterium clone NEP3-15 (98 % sequence similarity of 16S rRNA gene) derived from the phycosphere of Enteromorphaprolifera. The respiratory quinone was ubiquinone Q-8. The predominant fatty acids consisted of summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c), C16 : 0 and summed feature 3 (C16 : 1 ω6c/C16 : 1ω7c). The polar lipids were identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified amino phospholipid and four unknown lipids. The genomic DNA G+C content was 50.5 mol%. On the basis of polyphasic characterizations, strain feces2 represented a novel species and a novel genus in the family Cellvibrionaceae of the order Cellvibrionales within the Gammaproteobacteria, for which the name Agaribacteriumhaliotis gen. nov., sp. nov. is proposed, with the type strain being feces2 (=MCCC 1A11450 =KCTC 52708 ).
[Mh] Termos MeSH primário: Gammaproteobacteria/classificação
Gastrópodes/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Fezes/microbiologia
Gammaproteobacteria/genética
Gammaproteobacteria/isolamento & purificação
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002199


  10 / 1732 MEDLINE  
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[PMID]:28884674
[Au] Autor:Liu J; Sun YW; Li SN; Zhang DC
[Ad] Endereço:1​Department of Bioengineering, College of Marine Sciences and Biological Engineering, Qingdao University of Science and Technology, 266042, PR China.
[Ti] Título:Thalassotaleaprofundi sp. nov. isolated from a deep-sea seamount.
[So] Source:Int J Syst Evol Microbiol;67(10):3739-3743, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Gram-stain-negative, rod-shaped, strictly aerobic, motile bacterial strain, designated YM155 , was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YM155 was related to the genus Thalassotalea and had highest 16S rRNA gene sequence similarities with the type strains of Thalassotalea piscium T202 (97.2 %) and Thalassotalea agariperforans M-M1 (97.2 %). The predominant cellular fatty acids were C17 : 1ω8c, summed feature 3 (composed of iso-C15 : 0 2-OH and/or C16 : 1ω7c) and iso-C16 : 0. Ubiquinone 8 (Q-8) was the respiratory quinone. The polar lipid profile contained phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and one unidentified lipid. The genomic DNA G+C content of strain YM155 was 36.1 mol%. On the basis of the evidence presented in this study, strain YM155 represents a novel species of the genus Thalassotalea, for which we propose the name Thalassotalea profundi sp. nov. (type strain YM155 =KACC 18563 =CGMCC 1.15922 ).
[Mh] Termos MeSH primário: Gammaproteobacteria/classificação
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Gammaproteobacteria/genética
Gammaproteobacteria/isolamento & purificação
Oceano Pacífico
Fosfatidilgliceróis/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Ubiquinona/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phosphatidylglycerols); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 1339-63-5 (Ubiquinone); CQA993F7P8 (ubiquinone 8)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002180



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