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Pesquisa : B03.660.250.110 [Categoria DeCS]
Referências encontradas : 413 [refinar]
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[PMID]:29264600
[Au] Autor:Breglia R; Greco C; Fantucci P; De Gioia L; Bruschi M
[Ad] Endereço:Department of Earth and Environmental Science, University of Milano Bicocca, Piazza della Scienza 1, 20126 Milan, Italy. maurizio.bruschi@unimib.it.
[Ti] Título:Theoretical investigation of aerobic and anaerobic oxidative inactivation of the [NiFe]-hydrogenase active site.
[So] Source:Phys Chem Chem Phys;20(3):1693-1706, 2018 Jan 17.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The extraordinary capability of [NiFe]-hydrogenases to catalyse the reversible interconversion of protons and electrons into dihydrogen (H ) has stimulated numerous experimental and theoretical studies addressing the direct utilization of these enzymes in H production processes. Unfortunately, the introduction of these natural H -catalysts in biotechnological applications is limited by their inhibition under oxidising (aerobic and anaerobic) conditions. With the aim of contributing to overcome this limitation, we studied the oxidative inactivation mechanism of [NiFe]-hydrogenases by performing Density Functional Theory (DFT) calculations on a very large model of their active site in which all the amino acids forming the first and second coordination spheres of the NiFe cluster have been explicitly included. We identified an O molecule and two H O molecules as sources of the two oxygen atoms that are inserted at the active site of the inactive forms of the enzyme (Ni-A and Ni-B) under aerobic and anaerobic conditions, respectively. Furthermore, our results support the experimental evidence that the Ni-A-to-Ni-B ratio strongly depends on the number of reducing equivalents available for the process and on the oxidizing conditions under which the reaction takes place.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Hidrogenase/química
Modelos Moleculares
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Biocatálise
Domínio Catalítico
Chromatiaceae/enzimologia
Hidrogênio/química
Hidrogenase/metabolismo
Oxirredução
Oxigênio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 7YNJ3PO35Z (Hydrogen); EC 1.12.- (nickel-iron hydrogenase); EC 1.12.7.2 (Hydrogenase); S88TT14065 (Oxygen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp06228a


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[PMID]:29364601
[Au] Autor:Lunina ON; Savvichev AS; Krasnova ED; Kokryatskaya NM; Veslopolova EF; Kuznetsov BB; Gorlenko VM
[Ti] Título:Succession Processes in the Anoxygenic Phototrophic Bacterial Community in Lake Kislo-Sladkoe (Kandalaksha Bay, White Sea).
[So] Source:Mikrobiologiia;85(5):531-544, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The community of anoxygenic phototrophic bacteria (APB) in the water column of Lake Kislo- Sladkoe (Kandalaksha Bay, White Sea), which has recently become separated from the sea, was investigated in March-April 2012, March-April 2013, and in September 2013. The lake, which was previously considered meromictic, was in fact mixed and was strongly affected by the sea. In winter the lake is sometimes washed off with seawater, and this together with the seasonal cycles of succession processes determines the succession of the community. The consequences of the mixing in autumn 2011 could be observed in the APB community as late as autumn 2013. Green-colored green sulfur bacteria (GSB) usually predominated in the chemocline. In winter 2013 stagnation resulted in turbidity of water under the ice, which was responsible for both predom- inance of the brown GS B forms and the changes ratio of the species of purple sulfur bacteria (PS B) in anoxic water layers. Production of anoxygenic photosynthesis in the lake was at least 240 mg C m-2 day-- in September and 0-20 mg C m-2 day- in March-April, which corresponded to 40 and 69%, respectively, of oxygenic photosynthesis. Okenone-containing purple sulfur bacteria, strain TcakPS12 were isolated in 2012 from lake water. The ells of this strain form filaments of not separated cells. Strain TcakPS12 exhibited 98% similarity with the type strains of Thiocapsapendens DSM.236 and Thiocapsa bogorovii BBS, as well as with the strains AmPS10 and TcyrPS 10, which were isolated from Lake Kislo-Sladkoe in 2010.
[Mh] Termos MeSH primário: Baías/microbiologia
Chlorobi/genética
Chromatiaceae/genética
Lagos/microbiologia
Consórcios Microbianos/fisiologia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Chlorobi/classificação
Chlorobi/isolamento & purificação
Chlorobi/ultraestrutura
Chromatiaceae/classificação
Chromatiaceae/isolamento & purificação
Chromatiaceae/ultraestrutura
Ecossistema
Fotossíntese/fisiologia
Filogenia
Pigmentos Biológicos/isolamento & purificação
Federação Russa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pigments, Biological); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:29364597
[Au] Autor:Solov'ev AA; Ashikhmin AA; Moskalenko AA
[Ti] Título:Formation of a Subunit Form of the Core Light-Harvesting Complex from Sulfur Purple Bacteria Ectothiorhodospira haloalkaliphila with Different Carotenoid Composition.
[So] Source:Mikrobiologiia;85(5):497-505, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:B820 subunits from a purple sulfur bacterium Ectothiorhodospira. haloalkaliphila strain ATCC 51935T were obtained by treatment of Carotenoid free LH I-RC complexes of this bacterium with P--octylglu- copyranoside (ß-OG). The same complexes with 100% carotenoid content were unable to dissociate to B820 subunits, but disintegrated to monomeric bacteriochlorophyll (BChl) regardless of their carotenoid compo- sition. The degree of dissociation of the LH 1-RC complexes with an intermediate content of carotenoids (the' B820 formation) was directly dependent on the quantity of carotenoids in the samples. The resulting B820 subunits did not contain carotenoids. B820 subunits easily aggregated to form a complex with an absorption . peak at 880 nm at decreased ß-OG concentration. Analysis of the spectra of the LH I-RC complexes isolated from the cells with different'levels of carotenogenesis inhibition led to the conclusion of the heterogeneity of the samples with a predominance in them of (a) the fraction with 100% of carotenoids and (b) the fraction of carotenoid free complexes.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Carotenoides/isolamento & purificação
Chromatiaceae/química
Ectothiorhodospiraceae/química
Complexos de Proteínas Captadores de Luz/química
Subunidades Proteicas/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/isolamento & purificação
Bacterioclorofilas/química
Bacterioclorofilas/isolamento & purificação
Carotenoides/química
Carotenoides/classificação
Chromatiaceae/metabolismo
Detergentes/química
Ectothiorhodospiraceae/metabolismo
Glucosídeos/química
Complexos de Proteínas Captadores de Luz/isolamento & purificação
Extração Líquido-Líquido/métodos
Agregados Proteicos
Subunidades Proteicas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacteriochlorophylls); 0 (Detergents); 0 (Glucosides); 0 (Light-Harvesting Protein Complexes); 0 (Protein Aggregates); 0 (Protein Subunits); 29836-26-8 (octyl-beta-D-glucoside); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:29245157
[Au] Autor:Danza F; Storelli N; Roman S; Lüdin S; Tonolla M
[Ad] Endereço:Laboratory of Applied Microbiology (LMA), Department for Environmental Constructions and Design (DACD), University of Applied Sciences and Arts of Southern Switzerland (SUPSI), via Mirasole 22a, Bellinzona, Switzerland.
[Ti] Título:Dynamic cellular complexity of anoxygenic phototrophic sulfur bacteria in the chemocline of meromictic Lake Cadagno.
[So] Source:PLoS One;12(12):e0189510, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The meromictic Lake Cadagno is characterized by a compact chemocline with high concentrations of anoxygenic phototrophic purple sulfur bacteria (PSB) and green sulfur bacteria (GSB). The co-occurrence of phylogenetically distant bacterial groups such as PSB and GSB in the same ecological niche, makes the chemocline of Lake Cadagno an ideal system for studying the conditions and consequences of coexistence of photosynthetic bacteria populations. In this study, we applied flow cytometry (FCM) as a fast tool to identify metabolic changes due to the production and consumption of inclusion bodies such as sulfur globules (SGBs), and follow population dynamics of closely related anoxygenic photosynthetic sulfur bacteria in their natural environment. Large-celled PSB Chromatium okenii and GSB Chlorobium populations were reliably separated and identified due to differences in auto-fluorescence and cell size. Moreover, we showed that these dominant taxa share the same ecological niche over seasonal periods. Taking advantage of FCM detection of dynamic cellular complexity variation during phases of photosynthetic activity, we identified an unexpected alternation in PSB versus GSB metabolic activity, indicating dynamic interspecific interactions between these two populations.
[Mh] Termos MeSH primário: Chlorobi/fisiologia
Chromatiaceae/fisiologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Anaerobiose
DNA Bacteriano/metabolismo
Lagos/análise
Lagos/microbiologia
Oxirredução
Fotossíntese
RNA Bacteriano/metabolismo
Estações do Ano
Sulfetos/análise
Sulfetos/metabolismo
Suíça
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Bacterial); 0 (Sulfides)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189510


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[PMID]:28470424
[Au] Autor:Hong X; Chen Z; Zhao C; Yang S
[Ad] Endereço:Department of Bioengineering and Biotechnology, Huaqiao University, No. 668 Jimei Ave, Xiamen, 361021, People's Republic of China.
[Ti] Título:Nitrogen transformation under different dissolved oxygen levels by the anoxygenic phototrophic bacterium Marichromatium gracile.
[So] Source:World J Microbiol Biotechnol;33(6):113, 2017 Jun.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Marichromatium gracile: YL28 (M. gracile YL28) is an anoxygenic phototrophic bacterial strain that utilizes ammonia, nitrate, or nitrite as its sole nitrogen source during growth. In this study, we investigated the removal and transformation of ammonium, nitrate, and nitrite by M. gracile YL28 grown in a combinatorial culture system of sodium acetate-ammonium, sodium acetate-nitrate and sodium acetate-nitrite in response to different initial dissolved oxygen (DO) levels. In the sodium acetate-ammonium system under aerobic conditions (initial DO = 7.20-7.25 mg/L), we detected a continuous accumulation of nitrate and nitrite. However, under semi-anaerobic conditions (initial DO = 4.08-4.26 mg/L), we observed a temporary accumulation of nitrate and nitrite. Interestingly, under anaerobic conditions (initial DO = 0.36-0.67 mg/L), there was little accumulation of nitrate and nitrite, but an increase in nitrous oxide production. In the sodium acetate-nitrite system, nitrite levels declined slightly under aerobic conditions, and nitrite was completely removed under semi-anaerobic and anaerobic conditions. In addition, M. gracile YL28 was able to grow using nitrite as the sole nitrogen source in situations when nitrogen gas produced by denitrification was eliminated. Taken together, the data indicate that M. gracile YL28 performs simultaneous heterotrophic nitrification and denitrification at low-DO levels and uses nitrite as the sole nitrogen source for growth. Our study is the first to demonstrate that anoxygenic phototrophic bacteria perform heterotrophic ammonia-oxidization and denitrification under anaerobic conditions.
[Mh] Termos MeSH primário: Anaerobiose/fisiologia
Chromatiaceae/metabolismo
Nitrogênio/metabolismo
Oxigênio/metabolismo
Processos Fototróficos/fisiologia
[Mh] Termos MeSH secundário: Acetatos/metabolismo
Aerobiose/fisiologia
Amônia/metabolismo
Compostos de Amônio/metabolismo
Bactérias
Chromatiaceae/crescimento & desenvolvimento
Desnitrificação
Processos Heterotróficos/fisiologia
Cinética
Nitratos/metabolismo
Nitrificação
Nitritos/metabolismo
Óxido Nitroso/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Ammonium Compounds); 0 (Nitrates); 0 (Nitrites); 7664-41-7 (Ammonia); K50XQU1029 (Nitrous Oxide); N762921K75 (Nitrogen); RRE756S6Q2 (ammonium acetate); S88TT14065 (Oxygen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-017-2280-z


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[PMID]:28629500
[Au] Autor:Nupur N; Saini MK; Singh PK; Korpole S; Srinivas Tanuku NR; Takaichi S; Pinnaka AK
[Ad] Endereço:1​Microbial Type Culture Collection and Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh 160036, India.
[Ti] Título:Imhoffiella gen. nov., a marine phototrophic member of the family Chromatiaceae including the description of Imhoffiella purpurea sp. nov. and the reclassification of Thiorhodococcus bheemlicus Anil Kumar et al. 2007 as Imhoffiella bheemlica comb. nov.
[So] Source:Int J Syst Evol Microbiol;67(6):1949-1956, 2017 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A coccoid-shaped phototrophic purple sulfur bacterium, strain AK35T, was isolated from a coastal surface water sample collected from Visakhapatnam, India. Cells were Gram-stain-negative, motile and purple, containing bacteriochlorophyll a and the carotenoid rhodopinal as major photosynthetic pigments. Strain AK35T was able to grow photoheterotrophically and could utilize a number of organic substrates. It was unable to grow photoautotrophically. Strain AK35T was able to utilize sulfide and thiosulfate as electron donors. The main fatty acids present were identified as C16 : 0, C18 : 1ω7c, and C16 : 1ω7c and/or iso-C15 : 0 2OH (summed feature 3). Strain AK35T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and six unidentified lipids as polar lipids. The G+C content of the DNA of strain AK35T was 63.1 mol%. 16S rRNA gene sequence comparisons indicated that the isolate represented a member of the family Chromatiaceae. 16S rRNA gene sequence analysis indicated that strain AK35T is phylogenetically distinctly positioned outside the groups of most members of the genus Thiorhodococcus, clustered with members of the genera Marichromatium and Phaeochromatium, but was most closely related to Thiorhodococcus bheemlicus with a pairwise sequence similarity of 98.75 %. Based on DNA-DNA hybridization between strain AK35T and Thiorhodococcus bheemlicus MTCC 8120T a relatedness of 39.46 % was established. Distinct morphological, physiological and genotypic differences from these previously described taxa supported the classification of the new isolate as a representative of a novel species in a new genus, for which the name Imhoffiella purpurea gen. nov., sp. nov. is proposed. The type strain of Imhoffiella purpurea is AK35T (=JCM 18851T=KCTC 15575T=MTCC 12304T). In addition, Thiorhodococcus bheemlicus is recognized as another species of this genus and transferred to Imhoffiella bheemlica comb. nov.
[Mh] Termos MeSH primário: Chromatiaceae/classificação
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Bacterioclorofila A/química
Composição de Bases
Carotenoides/química
Chromatiaceae/genética
Chromatiaceae/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Índia
Hibridização de Ácido Nucleico
Fosfolipídeos/química
Pigmentação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacteriochlorophyll A); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001891


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[PMID]:28542634
[Au] Autor:Ohno H; Takeda K; Niwa S; Tsujinaka T; Hanazono Y; Hirano Y; Miki K
[Ad] Endereço:Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, Japan.
[Ti] Título:Crystallographic characterization of the high-potential iron-sulfur protein in the oxidized state at 0.8 Å resolution.
[So] Source:PLoS One;12(5):e0178183, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:High-potential iron-sulfur protein (HiPIP) is a soluble electron carrier protein of photosynthetic bacteria with an Fe4S4 cluster. Although structural changes accompanying the electron transfer are important for understanding of the functional mechanism, the changes have not been clarified in sufficient detail. We previously reported the high-resolution crystal structures of HiPIP from a thermophilic purple bacterium Thermochromatium tepidum in the reduced state. In order to perform a detailed comparison between the structures in different redox states, the oxidized structure should also be revealed at high resolution. Therefore, in the present study we performed a crystallographic analysis of oxidized HiPIP and a structural comparison with the reduced form at a high resolution of 0.8 Å. The comparison highlighted small but significant contraction in the iron-sulfur cluster. The changes in Fe-S bond lengths were similar to that predicted by theoretical calculation, although some discrepancies were also found. Almost distances between the sulfur atoms of the iron-sulfur cluster and the protein environment are elongated upon the oxidation. Positional changes of hydrogen atoms in the protein environment, such as on the amide-hydrogen of Cys75 in the proximity of the iron-sulfur cluster, were also observed in the accurate analyses. None of the water molecules exhibited significant changes in position or anisotropy of atomic displacement parameter between the two states, while the orientations of some water molecules were different.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Chromatiaceae/metabolismo
Proteínas com Ferro-Enxofre/química
Complexo de Proteínas do Centro de Reação Fotossintética/química
Conformação Proteica
[Mh] Termos MeSH secundário: Chromatiaceae/crescimento & desenvolvimento
Cristalografia por Raios X
Modelos Moleculares
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Iron-Sulfur Proteins); 0 (Photosynthetic Reaction Center Complex Proteins); 0 (high potential iron-sulfur protein)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178183


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[PMID]:28411470
[Au] Autor:Ashikhmin A; Makhneva Z; Bolshakov M; Moskalenko A
[Ad] Endereço:Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino 142290, Russia. Electronic address: AshikhminAA@gmail.com.
[Ti] Título:Incorporation of spheroidene and spheroidenone into light-harvesting complexes from purple sulfur bacteria.
[So] Source:J Photochem Photobiol B;170:99-107, 2017 May.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Spheroidene and spheroidenone from the non-sulfur bacterium Rhodobacter (Rba.) sphaeroides were incorporated into diphenylamine (DPA) LH1-RC and LH2 complexes from sulfur bacteria Allochromatium (Alc.) minutissimum and Ectothiorhodospira (Ect.) haloalkaliphila in which carotenoid (Car) biosynthesis was inhibited by ~95%. A series of biochemical characteristics of the modified LH2 complexes was studied (electrophoretic mobility, absorption and CD spectra, Car composition, Car-to-BChl energy transfer and thermal stability). It was found that the electrophoretic mobility of the complexes with incorporated Cars did not change compared to that of the control and DPA-complexes, indicating the absence of any significant change in the structure of LH complexes upon DPA-treatment and subsequent incorporation of Cars. The analysis of fluorescence excitation spectra of the spheroidene-incorporated LH2 complex (LH2:sph) and the spheroidenone-incorporated LH2 complex (LH2:sph-ne) showed that spheroidene and spheroidenone exhibited relatively low efficiencies of energy transfer to BChl, when incorporated into the LH2 DPA-complexes from Alc. minutissimum and Ect. haloalkaliphila, although, they showed high efficiencies, being in their natural state in the LH2 complexes from Rba. sphaeroides. A significant increase in thermostability observed for the LH2:sph and LH2:sph-ne complexes with respect to the LH2 DPA-complexes indicated that the two incorporated Cars stabilized the structure of the LH2 complexes.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Carotenoides/química
Chromatiaceae/metabolismo
Complexos de Proteínas Captadores de Luz/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Carotenoides/biossíntese
Carotenoides/farmacologia
Cromatografia Líquida de Alta Pressão
Dicroísmo Circular
Difenilamina/química
Transferência de Energia/efeitos dos fármacos
Complexos de Proteínas Captadores de Luz/análise
Complexos de Proteínas Captadores de Luz/química
Estabilidade Proteica
Espectrometria de Fluorescência
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Light-Harvesting Protein Complexes); 13836-61-8 (spheroidene); 13836-70-9 (spheroidenone); 36-88-4 (Carotenoids); 9N3CBB0BIQ (Diphenylamine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE


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[PMID]:28320886
[Au] Autor:Ryu MH; Fomicheva A; Moskvin OV; Gomelsky M
[Ad] Endereço:Department of Molecular Biology, University of Wyoming, Laramie, Wyoming, USA.
[Ti] Título:Optogenetic Module for Dichromatic Control of c-di-GMP Signaling.
[So] Source:J Bacteriol;199(18), 2017 Sep 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many aspects of bacterial physiology and behavior, including motility, surface attachment, and the cell cycle, are controlled by cyclic di-GMP (c-di-GMP)-dependent signaling pathways on the scale of seconds to minutes. Interrogation of such processes in real time requires tools for introducing rapid and reversible changes in intracellular c-di-GMP levels. Inducing the expression of genes encoding c-di-GMP-synthetic (diguanylate cyclases) and -degrading (c-di-GMP phosphodiesterase) enzymes by chemicals may not provide adequate temporal control. In contrast, light-controlled diguanylate cyclases and phosphodiesterases can be quickly activated and inactivated. A red/near-infrared-light-regulated diguanylate cyclase, BphS, was engineered previously, yet a complementary light-activated c-di-GMP phosphodiesterase has been lacking. In search of such a phosphodiesterase, we investigated two homologous proteins from and , designated BldP, which contain C-terminal EAL-BLUF modules, where EAL is a c-di-GMP phosphodiesterase domain and BLUF is a blue light sensory domain. Characterization of the BldP proteins in and showed that they possess light-activated c-di-GMP phosphodiesterase activities. Interestingly, light activation in both enzymes was dependent on oxygen levels. The truncated EAL-BLUF fragment from BldP lacked phosphodiesterase activity, whereas a similar fragment from BldP, designated EB1, possessed such activity that was highly (>30-fold) upregulated by light. Following light withdrawal, EB1 reverted to the inactive ground state with a half-life of ∼6 min. Therefore, the blue-light-activated phosphodiesterase EB1 can be used in combination with the red/near-infrared-light-regulated diguanylate cyclase BphS for the bidirectional regulation of c-di-GMP-dependent processes in as well as other bacterial and nonbacterial cells. Regulation of motility, attachment to surfaces, the cell cycle, and other bacterial processes controlled by the c-di-GMP signaling pathways occur at a fast (seconds-to-minutes) pace. Interrogation of these processes at high temporal and spatial resolution using chemicals is difficult or impossible, while optogenetic approaches may prove useful. We identified and characterized a robust, blue-light-activated c-di-GMP phosphodiesterase (hydrolase) that complements a previously engineered red/near-infrared-light-regulated diguanylate cyclase (c-di-GMP synthase). These two enzymes form a dichromatic module for manipulating intracellular c-di-GMP levels in bacterial and nonbacterial cells.
[Mh] Termos MeSH primário: GMP Cíclico/análogos & derivados
Escherichia coli/metabolismo
Escherichia coli/efeitos da radiação
Genética Microbiana/métodos
Optogenética/métodos
Diester Fosfórico Hidrolases/metabolismo
Transdução de Sinais
[Mh] Termos MeSH secundário: Alphaproteobacteria/enzimologia
Alphaproteobacteria/genética
Chromatiaceae/enzimologia
Chromatiaceae/genética
GMP Cíclico/metabolismo
Escherichia coli/enzimologia
Escherichia coli/genética
Luz
Diester Fosfórico Hidrolases/genética
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins); 61093-23-0 (bis(3',5')-cyclic diguanylic acid); EC 3.1.4.- (Phosphoric Diester Hydrolases); H2D2X058MU (Cyclic GMP)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE


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[PMID]:28176144
[Au] Autor:Kalinovskaya NI; Romanenko LA; Kalinovsky AI
[Ad] Endereço:G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, Russia, 690022.
[Ti] Título:Antibacterial low-molecular-weight compounds produced by the marine bacterium Rheinheimera japonica KMM 9513 .
[So] Source:Antonie Van Leeuwenhoek;110(5):719-726, 2017 May.
[Is] ISSN:1572-9699
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Strain KMM 9513 was isolated from a sediment sample collected from the Sea of Japan seashore and selected due to its ability to inhibit indicator bacterial growth. The strain KMM 9513 has been recently described as a novel species Rheinheimera japonica. This study was undertaken to determine which substances produced by strain KMM 9513 could be responsible for its antimicrobial activity. Eight compounds were obtained from an ethyl acetate extract of R. japonica KMM 9513 . The structures of five diketopiperazines (4-8) and diisobutyl-, dibutyl- and bis(2-ethylhexyl) phthalates (1-3) were established on the basis of detailed interpretation of NMR data, by Marfey method and optical rotation data. The structures of diketopiperazines were determined as cyclo-(L-valyl-L-proline), cyclo-(L-valyl-D-proline), cyclo-(L-phenylalanyl-L-proline), cyclo-(L-leucyl-L-proline), and cyclo-(L-phenylalanyl-D-proline). Compounds 1-3, 5 and 8 revealed antimicrobial activities against Bacillus subtilis and/or Enterococcus faecium and Staphylococcus aureus. In this paper, we describe the isolation and structural elucidation of the isolated compounds 1-8. This is the first report of the characterisation of low molecular weight antibacterial metabolites produced by a member of the genus Rheinheimera.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Chromatiaceae/química
Misturas Complexas/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/isolamento & purificação
Bacillus subtilis/efeitos dos fármacos
Técnicas de Química Analítica
Chromatiaceae/isolamento & purificação
Misturas Complexas/química
Misturas Complexas/isolamento & purificação
Enterococcus faecium/efeitos dos fármacos
Sedimentos Geológicos/microbiologia
Japão
Estrutura Molecular
Peso Molecular
Staphylococcus aureus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Complex Mixtures)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1007/s10482-017-0839-1



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