Base de dados : MEDLINE
Pesquisa : B04.100 [Categoria DeCS]
Referências encontradas : 173 [refinar]
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  1 / 173 MEDLINE  
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[PMID]:28878086
[Au] Autor:Weidenbach K; Nickel L; Neve H; Alkhnbashi OS; Künzel S; Kupczok A; Bauersachs T; Cassidy L; Tholey A; Backofen R; Schmitz RA
[Ad] Endereço:Christian Albrechts University, Institute for General Microbiology, Kiel, Germany.
[Ti] Título:Methanosarcina Spherical Virus, a Novel Archaeal Lytic Virus Targeting Methanosarcina Strains.
[So] Source:J Virol;91(22), 2017 Nov 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A novel archaeal lytic virus targeting species of the genus was isolated using strain Gö1 as the host. Due to its spherical morphology, the virus was designated hanosarcina pherical irus (MetSV). Molecular analysis demonstrated that MetSV contains double-stranded linear DNA with a genome size of 10,567 bp containing 22 open reading frames (ORFs), all oriented in the same direction. Functions were predicted for some of these ORFs, i.e., such as DNA polymerase, ATPase, and DNA-binding protein as well as envelope (structural) protein. MetSV-derived spacers in CRISPR loci were detected in several published draft genomes using bioinformatic tools, revealing a potential protospacer-adjacent motif (PAM) motif (TTA/T). Transcription and expression of several predicted viral ORFs were validated by reverse transcription-PCR (RT-PCR), PAGE analysis, and liquid chromatography-mass spectrometry (LC-MS)-based proteomics. Analysis of core lipids by atmospheric pressure chemical ionization (APCI) mass spectrometry showed that MetSV and both contain archaeol and glycerol dialkyl glycerol tetraether without a cyclopentane moiety (GDGT-0). The MetSV host range is limited to strains growing as single cells ( , and ). In contrast, strains growing as sarcina-like aggregates were apparently protected from infection. Heterogeneity related to morphology phases in cultures allowed acquisition of resistance to MetSV after challenge by growing cultures as sarcina-like aggregates. CRISPR/Cas-mediated resistance was excluded since neither of the two CRISPR arrays showed MetSV-derived spacer acquisition. Based on these findings, we propose that changing the morphology from single cells to sarcina-like aggregates upon rearrangement of the envelope structure prevents infection and subsequent lysis by MetSV. Methanoarchaea are among the most abundant organisms on the planet since they are present in high numbers in major anaerobic environments. They convert various carbon sources, e.g., acetate, methylamines, or methanol, to methane and carbon dioxide; thus, they have a significant impact on the emission of major greenhouse gases. Today, very little is known about viruses specifically infecting methanoarchaea that most probably impact the abundance of methanoarchaea in microbial consortia. Here, we characterize the first identified -infecting virus (MetSV) and show a mechanism for acquiring resistance against MetSV. Based on our results, we propose that growth as sarcina-like aggregates prevents infection and subsequent lysis. These findings allow new insights into the virus-host relationship in methanogenic community structures, their dynamics, and their phase heterogeneity. Moreover, the availability of a specific virus provides new possibilities to deepen our knowledge of the defense mechanisms of potential hosts and offers tools for genetic manipulation.
[Mh] Termos MeSH primário: Vírus de Archaea/fisiologia
Methanosarcina/virologia
[Mh] Termos MeSH secundário: Methanosarcina/genética
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE


  2 / 173 MEDLINE  
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[PMID]:28846925
[Au] Autor:Wu L; Uldahl KB; Chen F; Benasutti H; Logvinski D; Vu V; Banda NK; Peng X; Simberg D; Moghimi SM
[Ad] Endereço:Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, People's Republic of China.
[Ti] Título:Interaction of extremophilic archaeal viruses with human and mouse complement system and viral biodistribution in mice.
[So] Source:Mol Immunol;90:273-279, 2017 Oct.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Archaeal viruses offer exceptional biophysical properties for modification and exploration of their potential in bionanotechnology, bioengineering and nanotherapeutic developments. However, the interaction of archaeal viruses with elements of the innate immune system has not been explored, which is a necessary prerequisite if their potential for biomedical applications to be realized. Here we show complement activation through lectin (via direct binding of MBL/MASPs) and alternative pathways by two extremophilic archaeal viruses (Sulfolobus monocaudavirus 1 and Sulfolobus spindle-shaped virus 2) in human serum. We further show some differences in initiation of complement activation pathways between these viruses. Since, Sulfolobus monocaudavirus 1 was capable of directly triggering the alternative pathway, we also demonstrate that the complement regulator factor H has no affinity for the viral surface, but factor H deposition is purely C3-dependent. This suggests that unlike some virulent pathogens Sulfolobus monocaudavirus 1 does not acquire factor H for protection. Complement activation with Sulfolobus monocaudavirus 1 also proceeds in murine sera through MBL-A/C as well as factor D-dependent manner, but C3 deficiency has no overall effect on viral clearance by organs of the reticuloendothelial system on intravenous injection. However, splenic deposition was significantly higher in C3 knockout animals compared with the corresponding wild type mice. We discuss the potential application of these viruses in biomedicine in relation to their complement activating properties.
[Mh] Termos MeSH primário: Vírus de Archaea/imunologia
Ativação do Complemento/imunologia
Via Alternativa do Complemento/imunologia
Imunidade Inata/imunologia
[Mh] Termos MeSH secundário: Adulto
Animais
Complemento C3/genética
Complemento C3/imunologia
Fator H do Complemento/imunologia
Extremófilos/imunologia
Feminino
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Microscopia Eletrônica de Transmissão
Sulfolobus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complement C3); 80295-65-4 (Complement Factor H)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE


  3 / 173 MEDLINE  
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[PMID]:28218714
[Au] Autor:Demina TA; Pietilä MK; Svirskaite J; Ravantti JJ; Atanasova NS; Bamford DH; Oksanen HM
[Ad] Endereço:Department of Biosciences and Institute of Biotechnology, Viikinkaari 9, FIN-00014, University of Helsinki, Helsinki, Finland. tatiana.demina@helsinki.fi.
[Ti] Título:HCIV-1 and Other Tailless Icosahedral Internal Membrane-Containing Viruses of the Family Sphaerolipoviridae.
[So] Source:Viruses;9(2), 2017 Feb 18.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Members of the virus family include both archaeal viruses and bacteriophages that possess a tailless icosahedral capsid with an internal membrane. The genera and comprise viruses that infect halophilic euryarchaea, whereas viruses of thermophilic bacteria belong to the genus . Both sequence-based and structural clustering of the major capsid proteins and ATPases of sphaerolipoviruses yield three distinct clades corresponding to these three genera. Conserved virion architectural principles observed in sphaerolipoviruses suggest that these viruses belong to the PRD1-adenovirus structural lineage. Here we focus on archaeal alphasphaerolipoviruses and their related putative proviruses. The highest sequence similarities among alphasphaerolipoviruses are observed in the core structural elements of their virions: the two major capsid proteins, the major membrane protein, and a putative packaging ATPase. A recently described tailless icosahedral haloarchaeal virus, icosahedral virus 1 (HCIV-1), has a double-stranded DNA genome and an internal membrane lining the capsid. HCIV-1 shares significant similarities with the other tailless icosahedral internal membrane-containing haloarchaeal viruses of the family . The proposal to include a new virus species, , into the genus was submitted to the International Committee on Taxonomy of Viruses (ICTV) in 2016.
[Mh] Termos MeSH primário: Vírus de Archaea/classificação
Vírus de Archaea/ultraestrutura
Bacteriófagos/classificação
Bacteriófagos/ultraestrutura
Filogenia
Vírion/ultraestrutura
[Mh] Termos MeSH secundário: Adenosina Trifosfatases/genética
Archaea/virologia
Vírus de Archaea/genética
Bacteriófagos/genética
Proteínas do Capsídeo/genética
Análise de Sequência de DNA
Thermus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Capsid Proteins); EC 3.6.1.- (Adenosine Triphosphatases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


  4 / 173 MEDLINE  
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[PMID]:27681128
[Au] Autor:Iranzo J; Koonin EV; Prangishvili D; Krupovic M
[Ad] Endereço:National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland, USA.
[Ti] Título:Bipartite Network Analysis of the Archaeal Virosphere: Evolutionary Connections between Viruses and Capsidless Mobile Elements.
[So] Source:J Virol;90(24):11043-11055, 2016 Dec 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Archaea and particularly hyperthermophilic crenarchaea are hosts to many unusual viruses with diverse virion shapes and distinct gene compositions. As is typical of viruses in general, there are no universal genes in the archaeal virosphere. Therefore, to obtain a comprehensive picture of the evolutionary relationships between viruses, network analysis methods are more productive than traditional phylogenetic approaches. Here we present a comprehensive comparative analysis of genomes and proteomes from all currently known taxonomically classified and unclassified, cultivated and uncultivated archaeal viruses. We constructed a bipartite network of archaeal viruses that includes two classes of nodes, the genomes and gene families that connect them. Dissection of this network using formal community detection methods reveals strong modularity, with 10 distinct modules and 3 putative supermodules. However, compared to similar previously analyzed networks of eukaryotic and bacterial viruses, the archaeal virus network is sparsely connected. With the exception of the tailed viruses related to bacteriophages of the order Caudovirales and the families Turriviridae and Sphaerolipoviridae that are linked to a distinct supermodule of eukaryotic and bacterial viruses, there are few connector genes shared by different archaeal virus modules. In contrast, most of these modules include, in addition to viruses, capsidless mobile elements, emphasizing tight evolutionary connections between the two types of entities in archaea. The relative contributions of distinct evolutionary origins, in particular from nonviral elements, and insufficient sampling to the sparsity of the archaeal virus network remain to be determined by further exploration of the archaeal virosphere. IMPORTANCE: Viruses infecting archaea are among the most mysterious denizens of the virosphere. Many of these viruses display no genetic or even morphological relationship to viruses of bacteria and eukaryotes, raising questions regarding their origins and position in the global virosphere. Analysis of 5,740 protein sequences from 116 genomes allowed dissection of the archaeal virus network and showed that most groups of archaeal viruses are evolutionarily connected to capsidless mobile genetic elements, including various plasmids and transposons. This finding could reflect actual independent origins of the distinct groups of archaeal viruses from different nonviral elements, providing important insights into the emergence and evolution of the archaeal virome.
[Mh] Termos MeSH primário: Archaea/virologia
Vírus de Archaea/classificação
Evolução Biológica
Elementos de DNA Transponíveis
Genoma Viral
Filogenia
[Mh] Termos MeSH secundário: Vírus de Archaea/genética
Bactérias/virologia
Bacteriófagos/classificação
Bacteriófagos/genética
Biologia Computacional
Redes Reguladoras de Genes
Plasmídeos/metabolismo
Vírion/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170528
[Lr] Data última revisão:
170528
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160930
[St] Status:MEDLINE


  5 / 173 MEDLINE  
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[PMID]:27632564
[Au] Autor:Demina TA; Atanasova NS; Pietilä MK; Oksanen HM; Bamford DH
[Ad] Endereço:Institute of Biotechnology and Department of Biosciences, University of Helsinki, Viikinkaari 9, 00014, Finland.
[Ti] Título:Vesicle-like virion of Haloarcula hispanica pleomorphic virus 3 preserves high infectivity in saturated salt.
[So] Source:Virology;499:40-51, 2016 Dec.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hypersaline environments that are subject to salinity changes are particularly rich in viruses. Here we report a newly isolated archaeal halovirus, Haloarcula hispanica pleomorphic virus 3 (HHPV3). Its reproduction significantly retards host growth and decreases cell viability without causing lysis. HHPV3 particles require a minimum of 3M NaCl for stability and maintain high infectivity even in saturated salt. Notably, virions are irreversibly inactivated at ~1.5M NaCl in neutral pH, but tolerate this salinity at alkaline pH. The HHPV3 virion is a pleomorphic membrane vesicle containing two major protein species and lipids acquired nonselectively from the host membrane. The circular double-stranded DNA genome contains a conserved gene block characteristic of pleolipoviruses. We propose that HHPV3 is a member of the Betapleolipovirus genus (family Pleolipoviridae). Our findings add insights into the diversity observed among the pleolipoviruses found in hypersaline environments.
[Mh] Termos MeSH primário: Vírus de Archaea/fisiologia
Haloarcula/virologia
Salinidade
Vírion
[Mh] Termos MeSH secundário: Vírus de Archaea/isolamento & purificação
Vírus de Archaea/ultraestrutura
Ordem dos Genes
Genoma Viral
Especificidade de Hospedeiro
Concentração de Íons de Hidrogênio
Íons
Fases de Leitura Aberta
Vírion/isolamento & purificação
Vírion/fisiologia
Vírion/ultraestrutura
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ions)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160916
[St] Status:MEDLINE


  6 / 173 MEDLINE  
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[PMID]:27137505
[Au] Autor:Wang Y; Sima L; Lv J; Huang S; Liu Y; Wang J; Krupovic M; Chen X
[Ad] Endereço:State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
[Ti] Título:Identification, Characterization, and Application of the Replicon Region of the Halophilic Temperate Sphaerolipovirus SNJ1.
[So] Source:J Bacteriol;198(14):1952-64, 2016 Jul 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: The temperate haloarchaeal virus SNJ1 displays lytic and lysogenic life cycles. During the lysogenic cycle, the virus resides in its host, Natrinema sp. strain J7-1, in the form of an extrachromosomal circular plasmid, pHH205. In this study, a 3.9-kb region containing seven predicted genes organized in two operons was identified as the minimal replicon of SNJ1. Only RepA, encoded by open reading frame 11-12 (ORF11-12), was found to be essential for replication, and its expression increased during the lytic cycle. Sequence analysis suggested that RepA is a distant homolog of HUH endonucleases, a superfamily that includes rolling-circle replication initiation proteins from various viruses and plasmids. In addition to RepA, two genetic elements located within both termini of the 3.9-kb replicon were also required for SNJ1 replication. SNJ1 genome and SNJ1 replicon-based shuttle vectors were present at 1 to 3 copies per chromosome. However, the deletion of ORF4 significantly increased the SNJ1 copy number, suggesting that the product of ORF4 is a negative regulator of SNJ1 abundance. Shuttle vectors based on the SNJ1 replicon were constructed and validated for stable expression of heterologous proteins, both in J7 derivatives and in Natrinema pallidum JCM 8980(T), suggesting their broad applicability as genetic tools for Natrinema species. IMPORTANCE: Archaeal viruses exhibit striking morphological diversity and unique gene content. In this study, the minimal replicon of the temperate haloarchaeal virus SNJ1 was identified. A number of ORFs and genetic elements controlling virus genome replication, maintenance, and copy number were characterized. In addition, based on the replicon, a novel expression shuttle vector has been constructed and validated for protein expression and purification in Natrinema sp. CJ7 and Natrinema pallidum JCM 8980(T) This study not only provided mechanistic and functional insights into SNJ1 replication but also led to the development of useful genetic tools to investigate SNJ1 and other viruses infecting Natrinema species as well as their hosts.
[Mh] Termos MeSH primário: Vírus de Archaea/genética
Replicon
[Mh] Termos MeSH secundário: Vírus de Archaea/fisiologia
Sequência de Bases
Replicação do DNA
Vetores Genéticos/genética
Vetores Genéticos/metabolismo
Halobacteriaceae/virologia
Lisogenia
Dados de Sequência Molecular
Fases de Leitura Aberta
Plasmídeos/genética
Plasmídeos/metabolismo
Origem de Replicação
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160504
[St] Status:MEDLINE
[do] DOI:10.1128/JB.00131-16


  7 / 173 MEDLINE  
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[PMID]:27053548
[Au] Autor:Uldahl KB; Jensen SB; Bhoobalan-Chitty Y; Martínez-Álvarez L; Papathanasiou P; Peng X
[Ad] Endereço:Danish Archaea Centre, Department of Biology, University of Copenhagen, Copenhagen Biocenter, Copenhagen, DenmarkUniversity of Maryland.
[Ti] Título:Life Cycle Characterization of Sulfolobus Monocaudavirus 1, an Extremophilic Spindle-Shaped Virus with Extracellular Tail Development.
[So] Source:J Virol;90(12):5693-9, 2016 Jun 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: We provide here, for the first time, insights into the initial infection stages of a large spindle-shaped archaeal virus and explore the following life cycle events. Our observations suggest that Sulfolobus monocaudavirus 1 (SMV1) exhibits a high adsorption rate and that virions adsorb to the host cells via three distinct attachment modes: nosecone association, body association, and body/tail association. In the body/tail association mode, the entire virion, including the tail(s), aligns to the host cell surface and the main body is greatly flattened, suggesting a possible fusion entry mechanism. Upon infection, the intracellular replication cycle lasts about 8 h, at which point the virions are released as spindle-shaped tailless particles. Replication of the virus retarded host growth but did not cause lysis of the host cells. Once released from the host and at temperatures resembling that of its natural habitat, SMV1 starts developing one or two tails. This exceptional property of undergoing a major morphological development outside, and independently of, the host cell has been reported only once before for the related Acidianus two-tailed virus. Here, we show that SMV1 can develop tails of more than 900 nm in length, more than quadrupling the total virion length. IMPORTANCE: Very little is known about the initial life cycle stages of viruses infecting hosts of the third domain of life, Archaea This work describes the first example of an archaeal virus employing three distinct association modes. The virus under study, Sulfolobus monocaudavirus 1, is a representative of the large spindle-shaped viruses that are frequently found in acidic hot springs. The results described here will add valuable knowledge about Archaea, the least studied domain in the virology field.
[Mh] Termos MeSH primário: Vírus de Archaea/fisiologia
Genoma Viral
Sulfolobus/virologia
Ligação Viral
[Mh] Termos MeSH secundário: Vírus de Archaea/genética
Vírus de Archaea/isolamento & purificação
Fontes Termais/virologia
Proteínas Virais/genética
Proteínas Virais/metabolismo
Vírion/fisiologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160408
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.00075-16


  8 / 173 MEDLINE  
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[PMID]:26927156
[Au] Autor:Svirskaite J; Oksanen HM; Daugelavicius R; Bamford DH
[Ad] Endereço:Department of Biosciences, Institute of Biotechnology, University of Helsinki, Viikinkaari 9, 00014 Helsinki, Finland. julija.svirskaite@helsinki.fi.
[Ti] Título:Monitoring Physiological Changes in Haloarchaeal Cell during Virus Release.
[So] Source:Viruses;8(3):59, 2016 Feb 24.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The slow rate of adsorption and non-synchronous release of some archaeal viruses have hindered more thorough analyses of the mechanisms of archaeal virus release. To address this deficit, we utilized four viruses that infect Haloarcula hispanica that represent the four virion morphotypes currently known for halophilic euryarchaeal viruses: (1) icosahedral internal membrane-containing SH1; (2) icosahedral tailed HHTV-1; (3) spindle-shaped His1; and (4) pleomorphic His2. To discern the events occurring as the progeny viruses exit, we monitored culture turbidity, as well as viable cell and progeny virus counts of infected and uninfected cultures. In addition to these traditional metrics, we measured three parameters associated with membrane integrity: the binding of the lipophilic anion phenyldicarbaundecaborane, oxygen consumption, and both intra- and extra-cellular ATP levels.
[Mh] Termos MeSH primário: Vírus de Archaea/fisiologia
Haloarcula/fisiologia
Haloarcula/virologia
Liberação de Vírus
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/análise
Compostos de Boro/metabolismo
Contagem de Células
Sobrevivência Celular
Consumo de Oxigênio
Espectrofotometria
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Boron Compounds); 51434-88-9 (phenyldicarbaundecaborane); 8L70Q75FXE (Adenosine Triphosphate)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE


  9 / 173 MEDLINE  
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[PMID]:26820561
[Au] Autor:Hanhijärvi KJ; Ziedaite G; Hæggström E; Bamford DH
[Ad] Endereço:Department of Physics, University of Helsinki, Helsinki, Finland. kalle.hanhijarvi@helsinki.fi.
[Ti] Título:Temperature and pH dependence of DNA ejection from archaeal lemon-shaped virus His1.
[So] Source:Eur Biophys J;45(5):435-42, 2016 Jul.
[Is] ISSN:1432-1017
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The archaeal virus His1 isolated from a hypersaline environment infects an extremely halophilic archaeon Haloarcula hispanica. His1 features a lemon-shaped capsid, which is so far found only in archaeal viruses. This unique capsid can withstand high salt concentrations, and can transform into a helical tube, which in turn is resistant to extremely harsh conditions. Hypersaline environments exhibit a wide range of temperatures and pH conditions, which present an extra challenge to their inhabitants. We investigated the influence of pH and temperature on DNA ejection from His1 virus using single-molecule fluorescence experiments. The observed number of ejecting viruses is constant in pH 5 to 9, while the ejection process is suppressed at pH below 5. Similarly, the number of ejections within 15-42 °C shows only a minor increase around 25-37 °C. The maximum velocity of single ejected DNA increases with temperature, in qualitative agreement with the continuum model of dsDNA ejection.
[Mh] Termos MeSH primário: Vírus de Archaea/metabolismo
DNA Viral/metabolismo
Temperatura Ambiente
[Mh] Termos MeSH secundário: Vírus de Archaea/genética
Vírus de Archaea/fisiologia
Capsídeo/metabolismo
Genômica
Interações Hospedeiro-Patógeno
Concentração de Íons de Hidrogênio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160129
[St] Status:MEDLINE
[do] DOI:10.1007/s00249-016-1112-7


  10 / 173 MEDLINE  
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[PMID]:26763997
[Au] Autor:Hochstein RA; Amenabar MJ; Munson-McGee JH; Boyd ES; Young MJ
[Ad] Endereço:Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, USA Thermal Biology Institute, Montana State University, Bozeman, Montana, USA.
[Ti] Título:Acidianus Tailed Spindle Virus: a New Archaeal Large Tailed Spindle Virus Discovered by Culture-Independent Methods.
[So] Source:J Virol;90(7):3458-68, 2016 Jan 13.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: The field of viral metagenomics has expanded our understanding of viral diversity from all three domains of life (Archaea, Bacteria, and Eukarya). Traditionally, viral metagenomic studies provide information about viral gene content but rarely provide knowledge about virion morphology and/or cellular host identity. Here we describe a new virus, Acidianus tailed spindle virus (ATSV), initially identified by bioinformatic analysis of viral metagenomic data sets from a high-temperature (80°C) acidic (pH 2) hot spring located in Yellowstone National Park, followed by more detailed characterization using only environmental samples without dependency on culturing. Characterization included the identification of the large tailed spindle virion morphology, determination of the complete 70.8-kb circular double-stranded DNA (dsDNA) viral genome content, and identification of its cellular host. Annotation of the ATSV genome revealed a potential three-domain gene product containing an N-terminal leucine-rich repeat domain, followed by a likely posttranslation regulatory region consisting of high serine and threonine content, and a C-terminal ESCRT-III domain, suggesting interplay with the host ESCRT system. The host of ATSV, which is most closely related to Acidianus hospitalis, was determined by a combination of analysis of cellular clustered regularly interspaced short palindromic repeat (CRISPR)/Cas loci and dual viral and cellular fluorescence in situ hybridization (viral FISH) analysis of environmental samples and confirmed by culture-based infection studies. This work provides an expanded pathway for the discovery, isolation, and characterization of new viruses using culture-independent approaches and provides a platform for predicting and confirming virus hosts. IMPORTANCE: Virus discovery and characterization have been traditionally accomplished by using culture-based methods. While a valuable approach, it is limited by the availability of culturable hosts. In this research, we report a virus-centered approach to virus discovery and characterization, linking viral metagenomic sequences to a virus particle, its sequenced genome, and its host directly in environmental samples, without using culture-dependent methods. This approach provides a pathway for the discovery, isolation, and characterization of new viruses. While this study used an acidic hot spring environment to characterize a new archaeal virus, Acidianus tailed spindle virus (ATSV), the approach can be generally applied to any environment to expand knowledge of virus diversity in all three domains of life.
[Mh] Termos MeSH primário: Acidianus/virologia
Vírus de Archaea/classificação
Proteínas do Capsídeo/genética
Genoma Viral/genética
Fontes Termais/virologia
[Mh] Termos MeSH secundário: Vírus de Archaea/genética
Vírus de Archaea/isolamento & purificação
Sequência de Bases
Mapeamento Cromossômico
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
DNA Circular/genética
DNA Viral/genética
Temperatura Alta
Hibridização in Situ Fluorescente
Metagenômica
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (DNA, Circular); 0 (DNA, Viral)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160115
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.03098-15



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