Base de dados : MEDLINE
Pesquisa : B04.123.150.700 [Categoria DeCS]
Referências encontradas : 232 [refinar]
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  1 / 232 MEDLINE  
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[PMID]:28988127
[Au] Autor:Solovieva EV; Myakinina VP; Kislichkina AA; Krasilnikova VM; Verevkin VV; Mochalov VV; Lev AI; Fursova NK; Volozhantsev NV
[Ad] Endereço:State Research Center for Applied Microbiology and Biotechnology, Obolensk 142279, Moscow Region, Russia.
[Ti] Título:Comparative genome analysis of novel Podoviruses lytic for hypermucoviscous Klebsiella pneumoniae of K1, K2, and K57 capsular types.
[So] Source:Virus Res;243:10-18, 2018 01 02.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Hypermucoviscous (HV) strains of capsular types K1, K2 and K57 are the most virulent representatives of the Klebsiella pneumoniae species. Eight novel bacteriophages lytic for HV K. pneumoniae were isolated and characterized. Three bacteriophages, KpV41, KpV475, and KpV71 were found to have a lytic activity against mainly K. pneumoniae of capsular type K1. Two phages, KpV74, and KpV763 were lytic for K2 capsular type K. pneumoniae, and the phage KpV767 was specific to K57-type K. pneumoniae only. Two more phages, KpV766, and KpV48 had no capsular specificity. The phage genomes consist of a linear double-stranded DNA of 40,395-44,623bp including direct terminal repeats of 180-246 bp. The G + C contents are 52.3-54.2 % that is slightly lower than that of genomes of K. pneumoniae strains being used for phage propagation. According to the genome structures, sequence similarity and phylogenetic data, the phages are classified within the genus Kp32virus and Kp34virus of subfamily Autographivirinae, family Podoviridae. In the phage genomes, genes encoding proteins with putative motifs of polysaccharide depolymerase were identified. Depolymerase genes of phages KpV71 and KpV74 lytic for hypermucoviscous K. pneumoniae of K1 and K2 capsular type, respectively, were cloned and expressed in Escherichia coli, and the recombinant gene products were purified. The specificity and polysaccharide-degrading activity of the recombinant depolymerases were demonstrated.
[Mh] Termos MeSH primário: Bacteriófagos/isolamento & purificação
Genoma Viral
Klebsiella pneumoniae/virologia
Podoviridae/isolamento & purificação
[Mh] Termos MeSH secundário: Cápsulas Bacterianas/genética
Cápsulas Bacterianas/metabolismo
Bacteriófagos/classificação
Bacteriófagos/genética
Ordem dos Genes
Klebsiella pneumoniae/genética
Klebsiella pneumoniae/metabolismo
Filogenia
Podoviridae/classificação
Podoviridae/genética
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


  2 / 232 MEDLINE  
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[PMID]:28929273
[Au] Autor:Su J; Sun H; Liu J; Guo Z; Fan G; Gu G; Wang G
[Ad] Endereço:Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, China.
[Ti] Título:Complete genome sequence of a novel lytic bacteriophage isolated from Ralstonia solanacearum.
[So] Source:Arch Virol;162(12):3919-3923, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A lytic podophage RSPI1 was isolated from tobacco field soil collected in Fujian Province, South China using host bacterium Ralstonia solanacearum Tb15-14. Whole genome sequencing of this phage was performed using the high-throughput Ion Torrent PGM Sequencer. The complete genome of RSPI1 was 43,211 bp in length with a mean DNA G + C content of 61.5%. A total of 48 open reading frames were identified with lengths ranging from 132 bp to 5,061 bp, of which, 11, 12 and 25 were identified as functional, structural and unknown genes, respectively. A BLAST analysis revealed that this phage genome had a query cover of 78-79% and a highest identity of 84% with four podophages that infect Burkholderia pseudomallei. Two neighbor-joining phylogenetic trees were constructed using phage DNA polymerase I and tail fiber protein sequences and showed that this phage is closely related to Burkholderia phage Bp-AMP1, and also related to several phages that infect Ralstonia solanacearum. These findings indicate that RSPI1 is a novel phage that infects the notorious plant pathogen Ralstonia solanacearum.
[Mh] Termos MeSH primário: Bacteriófagos/classificação
Bacteriófagos/isolamento & purificação
Podoviridae/classificação
Podoviridae/isolamento & purificação
Ralstonia solanacearum/virologia
[Mh] Termos MeSH secundário: Bacteriólise
Bacteriófagos/genética
Bacteriófagos/fisiologia
Composição de Bases
Burkholderia pseudomallei/virologia
China
Genoma Viral
Sequenciamento de Nucleotídeos em Larga Escala
Fases de Leitura Aberta
Filogenia
Podoviridae/genética
Podoviridae/fisiologia
Análise de Sequência de DNA
Homologia de Sequência
Microbiologia do Solo
Tabaco/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3555-2


  3 / 232 MEDLINE  
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[PMID]:28812171
[Au] Autor:Xing S; Zhang X; Sun Q; Wang J; Mi Z; Pei G; Huang Y; An X; Fu K; Zhou L; Zhao B; Tong Y
[Ad] Endereço:Life Science Institute, Hebei Normal University, Shijiazhuang, 050024, Hebei, China.
[Ti] Título:Complete genome sequence of a novel, virulent Ahjdlikevirus bacteriophage that infects Enterococcus faecium.
[So] Source:Arch Virol;162(12):3843-3847, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A novel virulent bacteriophage named vB_EfaP_IME199 that specifically infects Enterococcus faecium was isolated and characterized. Its optimal multiplicity of infection was 0.01, and it had a 30 minute outbreak period. High-throughput sequencing revealed that the phage has a dsDNA genome of 18,838 bp with 22 open reading frames. The genome has very low homology to all other bacteriophage sequences in the GenBank database. Run-off sequencing experiments confirmed that vB_EfaP_IME199 has short inverted terminal repeats. Phylogenetic analysis indicated that vB_EfaP_IME199 can be taxonomically classified as a new member of the genus Ahjdlikevirus of family Podoviridae.
[Mh] Termos MeSH primário: Bacteriófagos/classificação
Bacteriófagos/isolamento & purificação
Enterococcus faecium/virologia
Genoma Viral
Podoviridae/classificação
Podoviridae/isolamento & purificação
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Bacteriófagos/genética
DNA/química
DNA/genética
Sequenciamento de Nucleotídeos em Larga Escala
Fases de Leitura Aberta
Filogenia
Podoviridae/genética
Podoviridae/crescimento & desenvolvimento
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170817
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3503-1


  4 / 232 MEDLINE  
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[PMID]:28730979
[Au] Autor:Uchiyama J; Taniguchi M; Kurokawa K; Takemura-Uchiyama I; Ujihara T; Shimakura H; Sakaguchi Y; Murakami H; Sakaguchi M; Matsuzaki S
[Ad] Endereço:1​School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
[Ti] Título:Adsorption of Staphylococcus viruses S13' and S24-1 on Staphylococcus aureus strains with different glycosidic linkage patterns of wall teichoic acids.
[So] Source:J Gen Virol;98(8):2171-2180, 2017 Aug.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The group of phages belonging to the family Podoviridae, genus P68virus, including Staphylococcus viruses S13' and S24-1, are important because of their benefits in phage therapy against Staphylococcus aureus infections. The O-glycosidic linkage patterns of wall teichoic acids (WTAs) in S. aureus cell walls seem to be important for adsorption of this phage group. In this study, the adsorption of Staphylococcus viruses S13' and S24-1 to S. aureus was examined using strains with modified WTA glycosidic linkage patterns. We found that the ß-O-N-acetylglucosamine of WTAs was essential for S13' adsorption, while N-acetylglucosamine, regardless of the α- and ß-O-glycosidic linkages of the WTAs, was essential for S24-1 adsorption. Next, examining the binding activities of their receptor-binding proteins (RBPs) to cell walls with different WTA glycosidic patterns, the ß-O-N-acetylglucosamine of the WTAs was essential for S13' RBP binding, while N-acetylglucosamine, regardless of the α- and ß-O-glycosidic linkages of the WTAs, was essential for S24-1 RBP binding. Therefore, the results of the RBP binding assays were consistent with those of the phage adsorption assays. Bioinformatic analysis suggested that the RBPs of Staphylococcus viruses S13' and S24-1 were structurally similar to the RBPs of phage phi11 of thefamily Siphoviridae. Phylogenetic analysis of the RBPs indicated that two phylogenetic subclusters in the family Podoviridae were related to the glycosidic linkage patterns required for phage adsorption, possibly mediated by RBPs. We hope that this study will encourage the future development of therapeutic phages.
[Mh] Termos MeSH primário: Receptores Virais/metabolismo
Fagos de Staphylococcus/fisiologia
Staphylococcus aureus/virologia
Ácidos Teicoicos/metabolismo
Ligação Viral
[Mh] Termos MeSH secundário: Podoviridae/fisiologia
Receptores Virais/química
Ácidos Teicoicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Virus); 0 (Teichoic Acids)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000865


  5 / 232 MEDLINE  
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[PMID]:28409265
[Au] Autor:Lim JA; Heu S; Park J; Roh E
[Ad] Endereço:Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, 55365, Republic of Korea.
[Ti] Título:Genomic characterization of bacteriophage vB_PcaP_PP2 infecting Pectobacterium carotovorum subsp. carotovorum, a new member of a proposed genus in the subfamily Autographivirinae.
[So] Source:Arch Virol;162(8):2441-2444, 2017 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bacteriophage vB_PcaP_PP2 (PP2) is a novel virulent phage that infects the plant-pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. PP2 phage has a 41,841-bp double-stranded DNA encoding 47 proteins, and it was identified as a member of the family Podoviridae by transmission electron microscopy. Nineteen of its open reading frames (ORFs) show homology to functional proteins, and 28 ORFs have been characterized as hypothetical proteins. PP2 phage is homologous to Cronobacter phage vB_CskP_GAP227 and Dev-CD-23823. Based on phylogenetic analysis, PP2 and its homologous bacteriophages form a new group within the subfamily Autographivirinae in the family Podoviridae, suggesting the need to establish a new genus. No lysogenic-cycle-related genes or bacterial toxins were identified.
[Mh] Termos MeSH primário: Bacteriófagos/genética
Genoma Viral
Pectobacterium/virologia
Podoviridae/classificação
Podoviridae/genética
[Mh] Termos MeSH secundário: Toxinas Bacterianas/genética
Bacteriófagos/classificação
Bacteriófagos/isolamento & purificação
Bacteriófagos/patogenicidade
DNA Viral/genética
Lisogenia/genética
Microscopia Eletrônica de Transmissão
Fases de Leitura Aberta
Filogenia
Plantas/microbiologia
Podoviridae/isolamento & purificação
Podoviridae/ultraestrutura
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (DNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3349-6


  6 / 232 MEDLINE  
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[PMID]:28001438
[Au] Autor:Qin K; Ji X; Zhang C; Ding Y; Kuang A; Zhang S; Zhang Q; Lin L; Wei Y
[Ad] Endereço:a Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, People's Republic of China.
[Ti] Título:Isolation and characterization of wetland VSW-3, a novel lytic cold-active bacteriophage of Pseudomonas fluorescens.
[So] Source:Can J Microbiol;63(2):110-118, 2017 Feb.
[Is] ISSN:1480-3275
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:Wetlands are often called the "kidneys of the Earth" and contribute substantially to environmental improvement. Pseudomonas fluorescens is a major contaminant of milk products and causes the spoilage of refrigerated foods and fresh poultry. In this study, we isolated and characterized a lytic cold-active bacteriophage named VSW-3 together with P. fluorescens SW-3 cells from the Napahai wetland in China. Electron microscopy showed that VSW-3 had an icosahedral head (56 nm) and a tapering tail (20 nm × 12 nm) and a genome size of approximate 40 kb. On the basis of the top-scoring hits in the BLASTP analysis, VSW-3 showed a high degree of module similarity to the Pseudomonas phages Andromeda and Bf7. The latent and burst periods were 45 and 20 min, respectively, with an average burst size of 90 phage particles per infected cell. The pH and thermal stability of VSW-3 were also explored. The optimal pH was found to be 7.0 and the activity decreased rapidly when the temperature exceeded 60 °C. VSW-3 is a cold-active bacteriophage, hence, it is important to research its ability to prevent product contamination caused by P. fluorescens and to characterize its relationship with its host P. fluorescens in the future.
[Mh] Termos MeSH primário: Podoviridae/isolamento & purificação
Fagos de Pseudomonas/isolamento & purificação
Pseudomonas fluorescens/virologia
[Mh] Termos MeSH secundário: Temperatura Baixa
Podoviridae/crescimento & desenvolvimento
Fagos de Pseudomonas/crescimento & desenvolvimento
Zonas Úmidas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE
[do] DOI:10.1139/cjm-2016-0368


  7 / 232 MEDLINE  
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[PMID]:27942842
[Au] Autor:Liu Z; Wang M; Meng X; Li Y; Wang D; Jiang Y; Shao H; Zhang Y
[Ad] Endereço:College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
[Ti] Título:Isolation and Genome Sequencing of a Novel Pseudoalteromonas Phage PH1.
[So] Source:Curr Microbiol;74(2):212-218, 2017 Feb.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The family Pseudoalteromonas is highly adaptable to dissimilar ecological habitats and plays an important ecological role in the marine environment. In this study, a new Pseudoalteromonas phage PH1 was isolated from the Yellow Sea. To better understand the bacteriophage, its biological properties, including morphology, host range, growth phenotype, thermal and pH stability, and nucleic acid composition, were investigated in detail. The result showed that the phage PH1 is a Podoviridae-phage with an icosahedral head (60 nm of diameter) and a short tail (26 nm in length). The phage PH1 genome consists of 42,685 bp length double-stranded DNA with a G+C content of 42.24% and is predicted to have 55 open reading frames (ORFs) with an average length of 740 bp nucleotides each. The phage PH1 genome adds a new Podoviridae-phage genome to marine bacteriophage dataset, which will provide useful basic information for further molecular research on interaction mechanisms between bacteriophages and their hosts.
[Mh] Termos MeSH primário: Bacteriófagos/isolamento & purificação
Genoma Viral
Podoviridae/isolamento & purificação
Pseudoalteromonas/virologia
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Bacteriófagos/classificação
Bacteriófagos/genética
Bacteriófagos/ultraestrutura
Composição de Bases
DNA/química
DNA/genética
DNA Viral/química
DNA Viral/genética
Especificidade de Hospedeiro
Concentração de Íons de Hidrogênio
Fases de Leitura Aberta
Podoviridae/classificação
Podoviridae/genética
Podoviridae/ultraestrutura
Água do Mar/virologia
Temperatura Ambiente
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 9007-49-2 (DNA)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-016-1175-9


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[PMID]:27818282
[Au] Autor:Kwiatek M; Parasion S; Rutyna P; Mizak L; Gryko R; Niemcewicz M; Olender A; Lobocka M
[Ad] Endereço:Military Institute of Hygiene and Epidemiology, Lubelska Str. 2, 24-100 Pulawy, Poland. Electronic address: magda.kwiatek09@gmail.com.
[Ti] Título:Isolation of bacteriophages and their application to control Pseudomonas aeruginosa in planktonic and biofilm models.
[So] Source:Res Microbiol;168(3):194-207, 2017 Apr.
[Is] ISSN:1769-7123
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Pseudomonas aeruginosa is frequently identified as a cause of diverse infections and chronic diseases. It forms biofilms and has natural resistance to several antibiotics. Strains of this pathogen resistant to new-generation beta-lactams have emerged. Due to the difficulties associated with treating chronic P. aeruginosa infections, bacteriophages are amongst the alternative therapeutic options being actively researched. Two obligatorily lytic P. aeruginosa phages, vB_PaeM_MAG1 (MAG1) and vB_PaeP_MAG4 (MAG4), have been isolated and characterized. These phages belong to the PAK_P1likevirus genus of the Myoviridae family and the LIT1virus genus of the Podoviridae family, respectively. They adsorb quickly to their hosts (∼90% in 5 min), have a short latent period (15 min), and are stable during storage. Each individual phage propagated in approximately 50% of P. aeruginosa strains tested, which increased to 72.9% when phages were combined into a cocktail. While MAG4 reduced biofilm more effectively after a short time of treatment, MAG1 was more effective after a longer time and selected less for phage-resistant clones. A MAG1-encoded homolog of YefM antitoxin of the bacterial toxin-antitoxin system may contribute to the superiority of MAG1 over MAG4.
[Mh] Termos MeSH primário: Biofilmes
Fagos de Pseudomonas/isolamento & purificação
Pseudomonas aeruginosa/virologia
[Mh] Termos MeSH secundário: Simulação por Computador
Seres Humanos
Microscopia Eletrônica
Myoviridae/isolamento & purificação
Myoviridae/fisiologia
Plâncton
Podoviridae/isolamento & purificação
Podoviridae/fisiologia
Infecções por Pseudomonas/microbiologia
Infecções por Pseudomonas/prevenção & controle
Infecções por Pseudomonas/terapia
Fagos de Pseudomonas/genética
Fagos de Pseudomonas/fisiologia
Fagos de Pseudomonas/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170413
[Lr] Data última revisão:
170413
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161108
[St] Status:MEDLINE


  9 / 232 MEDLINE  
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[PMID]:27612493
[Au] Autor:Wang X; Liu J; Yu Z; Jin J; Liu X; Wang G
[Ad] Endereço:Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China University of Chinese Academy of Sciences, Beijing 100049, China.
[Ti] Título:Novel groups of cyanobacterial podovirus DNA polymerase (pol) genes exist in paddy waters in northeast China.
[So] Source:FEMS Microbiol Ecol;92(12), 2016 Dec.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, we surveyed cyanopodovirus DNA polymerase (pol) sequences in paddy waters using the culture-independent PCR and Sanger sequencing methods. Four paddy waters generated from a pot experiment with different soil types collected from op E: n paddy fields in northeast China were used in this study. A total of 438 DNA pol clones were identified as cyanopodoviruses. The clones from the paddy waters formed nine unique groups of cyanopodoviruses either exclusively or with clones from East Lake in China (subclusters α-1 to α-8 and cluster ß). None of the clones from open oceans or coastal waters fell into these unique groups. Additionally, the distribution proportions of the clones into different cyanopodovirus groups varied among paddy water samples, which suggested that the cyanopodovirus compositions were spatially distributed in the paddy fields. The comparison of clone libraries in different studies indicated that the diversity of cyanopodoviruses in paddy waters was comparable to the diversity in the open oceans but was less than the diversity in the coastal estuary of Chesapeake Bay. Non-metric multidimensional scaling analysis indicated that the cyanopodovirus communities in paddy waters were similar to those in lake freshwater but distinct from the communities in marine and coastal waters.
[Mh] Termos MeSH primário: Cianobactérias/virologia
DNA Polimerase Dirigida por DNA/genética
Podoviridae/classificação
Podoviridae/enzimologia
[Mh] Termos MeSH secundário: Sequência de Bases
China
DNA Viral/genética
Água Doce/virologia
Oceanos e Mares
Filogenia
Podoviridae/genética
Podoviridae/isolamento & purificação
Análise de Sequência de DNA
Solo
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Soil); EC 2.7.7.7 (DNA-Directed DNA Polymerase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160911
[St] Status:MEDLINE


  10 / 232 MEDLINE  
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[PMID]:27541819
[Au] Autor:Liu H; Liu X; Yi X; Liu R; Huang J
[Ad] Endereço:College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, China.
[Ti] Título:The complete genome sequence of PE3-1, a novel E. coli O153 phage.
[So] Source:Arch Virol;161(11):3291-4, 2016 Nov.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A novel virulent phage PE3-1 against E. coli O153 was isolated from an aeration tank in a wastewater treatment plant. Transmission electron microscopy images showed that phage PE3-1 had an icosahedral head and a short tail, which revealed that it was a member of the family Podoviridae of the order Caudovirales. The complete PE3-1 genome consisted of 39,093 bp and was a linear double-stranded DNA with an average GC content of 49.93 %. Phage PE3-1 showed homology to the T7-like phages in 48 open reading frames (ORFs), but it differed from previously reported E .coli phages in morphology and bioinformatics analysis. This indicated that phage PE3-1 is a new member of the genus T7 virus.
[Mh] Termos MeSH primário: Colífagos/genética
Colífagos/isolamento & purificação
DNA Viral/genética
Genoma Viral
Podoviridae/genética
Podoviridae/isolamento & purificação
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Composição de Bases
Reatores Biológicos/virologia
Colífagos/classificação
Colífagos/ultraestrutura
DNA/genética
DNA Viral/química
Microscopia Eletrônica de Transmissão
Podoviridae/classificação
Podoviridae/ultraestrutura
Homologia de Sequência
Vírion/ultraestrutura
Águas Residuais/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Waste Water); 9007-49-2 (DNA)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160820
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3005-6



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