Base de dados : MEDLINE
Pesquisa : B04.123.205.600.500 [Categoria DeCS]
Referências encontradas : 623 [refinar]
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  1 / 623 MEDLINE  
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[PMID]:28450173
[Au] Autor:Chandler JC; Schaeffer JW; Davidson M; Magzamen SL; Pérez-Méndez A; Reynolds SJ; Goodridge LD; Volckens J; Franklin AB; Shriner SA; Bisha B
[Ad] Endereço:National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, CO, USA.
[Ti] Título:A method for the improved detection of aerosolized influenza viruses and the male-specific (F+) RNA coliphage MS2.
[So] Source:J Virol Methods;246:38-41, 2017 Aug.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The detection of aerosolized viruses can serve as an important surveillance and control tool in agriculture, human health, and environmental settings. Here, we adapted an anion exchange resin-based method, initially developed to concentrate negatively charged viruses from water, to liquid impingement-based bioaerosol sampling. In this method, aerosolized viruses are collected in a 20ml liquid sample contained within widely used impingers, BioSamplers (SKC Inc., Eighty Four, PA), and further concentrated via adsorption to an anion exchange resin that is suspended within this liquid. Viral nucleic acids are then extracted from the resin to facilitate molecular analyses through a reduction in the effective sample volume. For this study, various quantities of two negatively charged viruses, type A and type B influenza viruses (FluMist Quadrivalent vaccine) and the male-specific (F+) RNA coliphage MS2 (MS2), were nebulized into a custom-built bioaerosolization chamber, and sampled using BioSamplers with and without anion exchange resin. Compared to direct testing of the BioSampler liquid, detection was improved by 6.77× and 3.33× for type A and type B influenza viruses, respectively, by using the anion exchange resin. For MS2, the anion exchange resin method allowed for an average improvement in detection of 8.26×.
[Mh] Termos MeSH primário: Microbiologia do Ar
Levivirus/isolamento & purificação
Orthomyxoviridae/isolamento & purificação
Virologia/métodos
[Mh] Termos MeSH secundário: Aerossóis
Resinas de Troca de Ânions
Seres Humanos
Levivirus/genética
Masculino
RNA Viral
Manejo de Espécimes/métodos
Virologia/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aerosols); 0 (Anion Exchange Resins); 0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  2 / 623 MEDLINE  
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[PMID]:29197270
[Au] Autor:Majiya H; Adeyemi OO; Stonehouse NJ; Millner P
[Ad] Endereço:School of Biomedical Sciences, University of Leeds, UK.
[Ti] Título:Photodynamic inactivation of bacteriophage MS2: The A-protein is the target of virus inactivation.
[So] Source:J Photochem Photobiol B;178:404-411, 2018 Jan.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Singlet oxygen mediated oxidation has been shown to be responsible for photodynamic inactivation (PDI) of viruses in solution with photosensitisers such as 5, 10, 15, 20-tetrakis (1-methyl-4-pyridinio) porphyrin tetra p-toluenesulfonate (TMPyP). The capsids of non-enveloped viruses, such as bacteriophage MS2, are possible targets for viral inactivation by singlet oxygen oxidation. Within the capsid (predominantly composed of coat protein), the A-protein acts as the host recognition and attachment protein. The A-protein has two domains; an α-helix domain and a ß-sheet domain. The α-helix domain is attached to the viral RNA genome inside the capsid while the ß-sheet domain, which is on the surface of the capsid, is believed to be the site for attachment to the host bacteria pilus during infection. In this study, 4 sequence-specific antibodies were raised against 4 sites on the A-protein. Changes induced by the oxidation of singlet oxygen were compared to the rate of PDI of the virus. Using these antibodies, our results suggest that the rate of PDI is relative to loss of antigenicity of two sites on the A-protein. Our data further showed that PDI caused aggregation of MS2 particles and crosslinking of MS2 coat protein. However, these inter- and intra-capsid changes did not correlate to the rate of PDI we observed in MS2. Possible modes of action are discussed as a means to gaining insight to the targets and mechanisms of PDI of viruses.
[Mh] Termos MeSH primário: Levivirus/fisiologia
Proteínas Virais/antagonistas & inibidores
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Anticorpos/imunologia
Luz
Fármacos Fotossensibilizantes/química
Fármacos Fotossensibilizantes/farmacologia
Porfirinas/química
Porfirinas/farmacologia
Estrutura Secundária de Proteína
Estrutura Terciária de Proteína
Oxigênio Singlete/química
Oxigênio Singlete/toxicidade
Proteínas Virais/imunologia
Proteínas Virais/metabolismo
Inativação de Vírus/efeitos dos fármacos
Inativação de Vírus/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (Photosensitizing Agents); 0 (Porphyrins); 0 (Viral Proteins); 0 (maturation protein, Enterobacterio phage MS2); 17778-80-2 (Singlet Oxygen); 38673-65-3 (tetra(4-N-methylpyridyl)porphine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE


  3 / 623 MEDLINE  
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[PMID]:29334364
[Au] Autor:Losdorfer Bozic A; Micheletti C; Podgornik R; Tubiana L
[Ad] Endereço:Department of Theoretical Physics, Jozef Stefan Institute, SI-1000 Ljubljana, Slovenia.
[Ti] Título:Compactness of viral genomes: effect of disperse and localized random mutations.
[So] Source:J Phys Condens Matter;30(8):084006, 2018 Feb 28.
[Is] ISSN:1361-648X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Genomes of single-stranded RNA viruses have evolved to optimize several concurrent properties. One of them is the architecture of their genomic folds, which must not only feature precise structural elements at specific positions, but also allow for overall spatial compactness. The latter was shown to be disrupted by random synonymous mutations, a disruption which can consequently negatively affect genome encapsidation. In this study, we use three mutation schemes with different degrees of locality to mutate the genomes of phage MS2 and Brome Mosaic virus in order to understand the observed sensitivity of the global compactness of their folds. We find that mutating local stretches of their genomes' sequence or structure is less disruptive to their compactness compared to inducing randomly-distributed mutations. Our findings are indicative of a mechanism for the conservation of compactness acting on a global scale of the genomes, and have several implications for understanding the interplay between local and global architecture of viral RNA genomes.
[Mh] Termos MeSH primário: Bromovirus/genética
Levivirus/genética
Conformação de Ácido Nucleico
[Mh] Termos MeSH secundário: Genoma Viral
RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
63231-63-0 (RNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180116
[St] Status:MEDLINE
[do] DOI:10.1088/1361-648X/aaa7b0


  4 / 623 MEDLINE  
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[PMID]:28800517
[Au] Autor:Kosel J; Gutiérrez-Aguirre I; Racki N; Dreo T; Ravnikar M; Dular M
[Ad] Endereço:Department of Power Engineering, Faculty of Mechanical Engineering, University of Ljubljana, Askerceva 6, 1000 Ljubljana, Slovenia; National Institute of Biology, Vecna pot 111, 1000 Ljubljana, Slovenia.
[Ti] Título:Efficient inactivation of MS-2 virus in water by hydrodynamic cavitation.
[So] Source:Water Res;124:465-471, 2017 Nov 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to accurately quantify the impact of hydrodynamic cavitation on the infectivity of bacteriophage MS2, a norovirus surrogate, and to develop a small scale reactor for testing the effect of hydrodynamic cavitation on human enteric viruses, which cannot be easily prepared in large quantities. For this purpose, 3 mL scale and 1 L scale reactors were constructed and tested. Both devices were efficient in generating hydrodynamic cavitation and in reducing the infectivity of MS2 virus. Furthermore, they reached more than 4 logs reductions of viral infectivity, thus confirming the scalability of hydrodynamic cavitation for this particular application. As for the mechanism of page inactivation, we suspect that cavitation generated OH radicals formed an advanced oxidation process, which could have damaged the host's recognition receptors located on the surface of the bacteriophage. Additional damage could arise from the high shear forces inside the cavity. Moreover, the effectiveness of the cavitation was higher for suspensions containing low initial viral titers that are in similar concentration to the ones found in real water samples. According to this, cavitation generators could prove to be a useful tool for treating virus-contaminated wastewaters in the future.
[Mh] Termos MeSH primário: Levivirus
Norovirus
Águas Residuais
[Mh] Termos MeSH secundário: Seres Humanos
Hidrodinâmica
Inativação de Vírus
Água
Purificação da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water); 059QF0KO0R (Water)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE


  5 / 623 MEDLINE  
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[PMID]:28783495
[Au] Autor:van Halem D; van der Laan H; Soppe AIA; Heijman SGJ
[Ad] Endereço:Delft University of Technology, Department of Water Management, Stevinweg 1, 2628 CN Delft, The Netherlands. Electronic address: d.vanhalem@tudelft.nl.
[Ti] Título:High flow ceramic pot filters.
[So] Source:Water Res;124:398-406, 2017 Nov 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ceramic pot filters are considered safe, robust and appropriate technologies, but there is a general consensus that water revenues are limited due to clogging of the ceramic element. The objective of this study was to investigate the potential of high flow ceramic pot filters to produce more water without sacrificing their microbial removal efficacy. High flow pot filters, produced by increasing the rice husk content, had a higher initial flow rate (6-19 L h ), but initial LRVs for E. coli of high flow filters was slightly lower than for regular ceramic pot filters. This disadvantage was, however, only temporarily as the clogging in high flow filters had a positive effect on the LRV for E. coli (from below 1 to 2-3 after clogging). Therefore, it can be carefully concluded that regular ceramic pot filters perform better initially, but after clogging, the high flow filters have a higher flow rate as well as a higher LRV for E. coli. To improve the initial performance of new high flow filters, it is recommended to further utilize residence time of the water in the receptacle, since additional E. coli inactivation was observed during overnight storage. Although a relationship was observed between flow rate and LRV of MS2 bacteriophages, both regular and high flow filters were unable to reach over 2 LRV.
[Mh] Termos MeSH primário: Cerâmica
Purificação da Água
[Mh] Termos MeSH secundário: Escherichia coli
Filtração
Levivirus
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE


  6 / 623 MEDLINE  
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[PMID]:28771128
[Au] Autor:Pourcel C; Midoux C; Vergnaud G; Latino L
[Ad] Endereço:Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France.
[Ti] Título:A carrier state is established in Pseudomonas aeruginosa by phage LeviOr01, a newly isolated ssRNA levivirus.
[So] Source:J Gen Virol;98(8):2181-2189, 2017 Aug.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:ssRNA bacteriophages are very abundant but poorly studied, particularly in relation to their effect on bacterial evolution. We isolated a new Pseudomonas aeruginosa levivirus, vB_PaeL_PcyII-10_LeviOr01, from hospital waste water. Its genome comprises 3669 nucleotides and encodes four putative proteins. Following bacterial infection, a carrier state is established in a fraction of the cells, conferring superinfection immunity. Such cells also resist other phages that use type IV pili as a receptor. The carrier population is composed of a mixture of cells producing phage, and susceptible cells that are non-carriers. Carrier cells accumulate phage until they burst, releasing large quantities of virions. The continuous presence of phage favours the emergence of host variants bearing mutations in genes involved in type IV pilus biogenesis, but also in genes affecting lipopolysaccharide (LPS) synthesis. The establishment of a carrier state in which phage particles are continuously released was previously reported for some dsRNA phages, but has not previously been described for a levivirus. The present results highlight the importance of the carrier state, an association that benefits both phages and bacteria and plays a role in bacterial evolution.
[Mh] Termos MeSH primário: Interações Hospedeiro-Parasita
Levivirus/fisiologia
Fagos de Pseudomonas/fisiologia
Pseudomonas aeruginosa/virologia
[Mh] Termos MeSH secundário: Genoma Viral
Levivirus/isolamento & purificação
Fagos de Pseudomonas/isolamento & purificação
RNA Viral/genética
Análise de Sequência de DNA
Liberação de Vírus
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000883


  7 / 623 MEDLINE  
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[PMID]:28523559
[Au] Autor:Czaplinski K
[Ad] Endereço:Center for Nervous System Disorders, Centers for Molecular Medicine, Stony Brook University, Stony Brook, NY, 11794, USA. Kevin.Czaplinski@stonybrook.edu.
[Ti] Título:Techniques for Single-Molecule mRNA Imaging in Living Cells.
[So] Source:Adv Exp Med Biol;978:425-441, 2017.
[Is] ISSN:0065-2598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Typical measurement of macromolecules in a biological sample typically averages the result over all the cells or molecules within the sample, and while these types of measurements provide very useful information, they completely miss heterogeneity among the components within the sample that could be a very important aspect of the sample's function. These techniques are also limited in their ability to examine intracellular spatial orientation of molecular activity, which is often a critical component to the regulation of biological processes, particularly in cells with unique spatial relationships, such as neurons. This makes a strong case for single-cell and single-molecule analysis that allows similar novel insight into complex molecular machinery that would not be possible when pooling heterogeneous molecular states. mRNA has proven to be quite tractable to molecular analysis in single cells. Almost two decades of single-molecule studies of mRNA processing both in situ and in live cells have been facilitated by microscopy of mRNA. This has been made possible by multiplexing fluorophores in situ hybridization probes or fluorescent RNA-tag-binding protein probes. The purpose of this chapter is to describe the approaches that have made single-molecule mRNA imaging accessible, as well as to give an overview of the state of the art for techniques that are available to track mRNA in real time in living cells, highlighting the application to neuroscience.
[Mh] Termos MeSH primário: Neurônios/química
RNA Mensageiro/análise
Imagem Individual de Molécula/métodos
[Mh] Termos MeSH secundário: Animais
Axônios/metabolismo
Axônios/ultraestrutura
Transporte Biológico
Proteínas do Capsídeo/análise
Proteínas do Capsídeo/genética
Dendritos/metabolismo
Dendritos/ultraestrutura
Corantes Fluorescentes/análise
Genes Reporter
Seres Humanos
Hibridização in Situ Fluorescente
Levivirus/genética
Neurônios/ultraestrutura
Biossíntese de Proteínas
RNA Mensageiro/metabolismo
Análise de Célula Única/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; REVIEW
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Fluorescent Dyes); 0 (RNA, Messenger)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE
[do] DOI:10.1007/978-3-319-53889-1_22


  8 / 623 MEDLINE  
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[PMID]:28445762
[Au] Autor:Ciocanel MV; Kreiling JA; Gagnon JA; Mowry KL; Sandstede B
[Ad] Endereço:Division of Applied Mathematics, Brown University, Providence, Rhode Island.
[Ti] Título:Analysis of Active Transport by Fluorescence Recovery after Photobleaching.
[So] Source:Biophys J;112(8):1714-1725, 2017 Apr 25.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fluorescence recovery after photobleaching (FRAP) is a well-established experimental technique to study binding and diffusion of molecules in cells. Although a large number of analytical and numerical models have been developed to extract binding and diffusion rates from FRAP recovery curves, active transport of molecules is typically not included in the existing models that are used to estimate these rates. Here we present a validated numerical method for estimating diffusion, binding/unbinding rates, and active transport velocities using FRAP data that captures intracellular dynamics through partial differential equation models. We apply these methods to transport and localization of mRNA molecules in Xenopus laevis oocytes, where active transport processes are essential to generate developmental polarity. By providing estimates of the effective velocities and diffusion, as well as expected run times and lengths, this approach can help quantify dynamical properties of localizing and nonlocalizing RNA. Our results confirm the distinct transport dynamics in different regions of the cytoplasm, and suggest that RNA movement in both the animal and vegetal directions may influence the timescale of RNA localization in Xenopus oocytes. We also show that model initial conditions extracted from FRAP postbleach intensities prevent underestimation of diffusion, which can arise from the instantaneous bleaching assumption. The numerical and modeling approach presented here to estimate parameters using FRAP recovery data is a broadly applicable tool for systems where intracellular transport is a key molecular mechanism.
[Mh] Termos MeSH primário: Transporte Biológico Ativo
Recuperação de Fluorescência Após Fotodegradação
Modelos Moleculares
[Mh] Termos MeSH secundário: Animais
Transporte Biológico Ativo/fisiologia
Proteínas do Capsídeo/metabolismo
Simulação por Computador
Citoplasma/metabolismo
Difusão
Levivirus
Proteínas Luminescentes/metabolismo
Microinjeções
Movimento (Física)
Oócitos/metabolismo
Ligação Proteica
RNA Mensageiro/metabolismo
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Luminescent Proteins); 0 (RNA, Messenger); 0 (red fluorescent protein)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE


  9 / 623 MEDLINE  
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Texto completo SciELO Brasil
[PMID]:28403327
[Au] Autor:Zambenedetti MR; Pavoni DP; Dallabona AC; Dominguez AC; Poersch CO; Fragoso SP; Krieger MA
[Ad] Endereço:Fundação Oswaldo Cruz-Fiocruz, Instituto Carlos Chagas, Laboratório de Genômica, Curitiba, PR, Brasil.
[Ti] Título:Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics.
[So] Source:Mem Inst Oswaldo Cruz;112(5):339-347, 2017 May.
[Is] ISSN:1678-8060
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Real-time reverse transcription polymerase chain reaction (RT-PCR) is routinely used to detect viral infections. In Brazil, it is mandatory the use of nucleic acid tests to detect hepatitis C virus (HCV), hepatitis B virus and human immunodeficiency virus in blood banks because of the immunological window. The use of an internal control (IC) is necessary to differentiate the true negative results from those consequent from a failure in some step of the nucleic acid test. OBJECTIVES: The aim of this study was the construction of virus-modified particles, based on MS2 bacteriophage, to be used as IC for the diagnosis of RNA viruses. METHODS: The MS2 genome was cloned into the pET47b(+) plasmid, generating pET47b(+)-MS2. MS2-like particles were produced through the synthesis of MS2 RNA genome by T7 RNA polymerase. These particles were used as non-competitive IC in assays for RNA virus diagnostics. In addition, a competitive control for HCV diagnosis was developed by cloning a mutated HCV sequence into the MS2 replicase gene of pET47b(+)-MS2, which produces a non-propagating MS2 particle. The utility of MS2-like particles as IC was evaluated in a one-step format multiplex real-time RT-PCR for HCV detection. FINDINGS: We demonstrated that both competitive and non-competitive IC could be successfully used to monitor the HCV amplification performance, including the extraction, reverse transcription, amplification and detection steps, without compromising the detection of samples with low target concentrations. In conclusion, MS2-like particles generated by this strategy proved to be useful IC for RNA virus diagnosis, with advantage that they are produced by a low cost protocol. An attractive feature of this system is that it allows the construction of a multicontrol by the insertion of sequences from more than one pathogen, increasing its applicability for diagnosing different RNA viruses.
[Mh] Termos MeSH primário: Hepatite C/diagnóstico
Levivirus/genética
Vírus de RNA/genética
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Escherichia coli/genética
Hepacivirus/genética
Modelos Biológicos
Padrões de Referência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE


  10 / 623 MEDLINE  
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[PMID]:28399477
[Au] Autor:Mac Mahon J; Pillai SC; Kelly JM; Gill LW
[Ad] Endereço:Department of Civil, Structural and Environmental Engineering, Museum Building, Trinity College Dublin, College Green, Dublin 2, Ireland. Electronic address: macmahoj@tcd.ie.
[Ti] Título:Solar photocatalytic disinfection of E. coli and bacteriophages MS2, ΦX174 and PR772 using TiO , ZnO and ruthenium based complexes in a continuous flow system.
[So] Source:J Photochem Photobiol B;170:79-90, 2017 May.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The performance of photocatalytic treatment processes were assessed using different photocatalysts against E. coli and bacteriophages MS2, ΦX174 and PR772, in a recirculating continuous flow compound parabolic collector system under real sunlight conditions. Suspended TiO and ZnO nanoparticle powders and Tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate in solution were tested separately, as well as in combination, using E. coli. For a 3-log reduction of E. coli in distilled water, inactivation rates in terms of cumulative dose were in the order Ru(bpy) Cl >(TiO & Ru(bpy) Cl )>(ZnO & Ru(bpy) Cl )>ZnO>TiO >photolysis. Reactivation of E. coli was observed following all trials despite the detection limit being reached, although the reactivated colonies were observed to be under stress and much slower growing when compared to original colonies. Treatment with Ru(bpy) Cl was also compared against standard photolysis of bacteriophages MS2, ΦX174 and PR772 with the order of photolytic inactivation for a 3-log reduction in terms of cumulative UV-A dose being ΦX174>PR772>MS2. However, MS2 was found to be the most susceptible bacteriophage to treatment with Ru(bpy) Cl , with complete removal of the phage observed within the first 15min of exposure. Ru(bpy) Cl also significantly improved inactivation rates for PR772 and ΦX174.
[Mh] Termos MeSH primário: Bacteriófagos/efeitos dos fármacos
Desinfetantes/farmacologia
Escherichia coli/efeitos dos fármacos
Fotólise/efeitos dos fármacos
Raios Ultravioleta
[Mh] Termos MeSH secundário: Bacteriófago phi X 174/efeitos dos fármacos
Bacteriófago phi X 174/efeitos da radiação
Bacteriófagos/efeitos da radiação
Catálise
Desinfetantes/química
Escherichia coli/efeitos da radiação
Levivirus/efeitos dos fármacos
Levivirus/efeitos da radiação
Compostos Organometálicos/química
Compostos Organometálicos/farmacologia
Fotólise/efeitos da radiação
Titânio/química
Titânio/farmacologia
Inativação de Vírus/efeitos dos fármacos
Inativação de Vírus/efeitos da radiação
Purificação da Água
Óxido de Zinco/química
Óxido de Zinco/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disinfectants); 0 (Organometallic Compounds); 0 (tris(2,2'-bipyridyl)ruthenium(II)); 15FIX9V2JP (titanium dioxide); D1JT611TNE (Titanium); SOI2LOH54Z (Zinc Oxide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE



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