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[PMID]:28934297
[Au] Autor:Ahmad AA; Stulberg MJ; Mershon JP; Mollov DS; Huang Q
[Ad] Endereço:Floral and Nursery Plants Research Unit, United States National Arboretum, U.S. Dept. of Agriculture-Agricultural Research Service, Beltsville, Maryland, United States of America.
[Ti] Título:Molecular and biological characterization of Ï•Rs551, a filamentous bacteriophage isolated from a race 3 biovar 2 strain of Ralstonia solanacearum.
[So] Source:PLoS One;12(9):e0185034, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A filamentous bacteriophage, designated Ï•Rs551, was isolated and purified from the quarantine and select agent phytopathogen Ralstonia solanacearum race 3 biovar 2 strain UW551 (phylotype IIB sequevar 1) grown under normal culture conditions. Electron microscopy suggested that Ï•Rs551 is a member of the family Inoviridae, and is about 1200 nm long and 7 nm wide. Ï•Rs551 has a genome of 7929 nucleotides containing 14 open reading frames, and is the first isolated virion that contains a resolvase (ORF13) and putative type-2 phage repressor (ORF14). Unlike other R. solanacearum phages isolated from soil, the genome sequence of Ï•Rs551 is not only 100% identical to its prophage sequence in the deposited genome of R. solanacearum strain UW551 from which the phage was isolated, but is also surprisingly found with 100% identity in the deposited genomes of 10 other phylotype II sequevar 1 strains of R. solanacearum. Furthermore, it is homologous to genome RS-09-161, resulting in the identification of a new prophage, designated RSM10, in a R. solanacearum strain from India. When ORF13 and a core attP site of Ï•Rs551 were either deleted individually or in combination, phage integration was not observed, suggesting that similar to other filamentous R. solanacearum Ï•RSM phages, Ï•Rs551 relies on its resolvase and the core att sequence for site-directed integration into its susceptible R. solanacearum strain. The integration occurred four hours after phage infection. Infection of a susceptible R. solanacearum strain RUN302 by Ï•Rs551 resulted in less fluidal colonies and EPS production, and reduced motilities of the bacterium. Interestingly, infection of RUN302 by Ï•Rs551 also resulted in reduced virulence, rather than enhanced or loss of virulence caused by other Ï•RSM phages. Study of bacteriophages of R. solanacearum would contribute to a better understanding of the phage-bacterium-environment interactions in order to develop integrated management strategies to combat R. solanacearum.
[Mh] Termos MeSH primário: Genoma Viral
Inovirus/genética
Inovirus/isolamento & purificação
Doenças das Plantas/virologia
Ralstonia solanacearum/virologia
Virulência/genética
[Mh] Termos MeSH secundário: DNA Viral/genética
Índia
Inovirus/metabolismo
Filogenia
Prófagos/genética
Ralstonia solanacearum/crescimento & desenvolvimento
Ralstonia solanacearum/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185034


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[PMID]:28397779
[Au] Autor:Loh B; Haase M; Mueller L; Kuhn A; Leptihn S
[Ad] Endereço:Sebastian Leptihn, Institute of Microbiology and Molecular Biology, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, Germany. belinda.loh@uni-hohenheim.de.
[Ti] Título:The Transmembrane Morphogenesis Protein gp1 of Filamentous Phages Contains Walker A and Walker B Motifs Essential for Phage Assembly.
[So] Source:Viruses;9(4), 2017 Apr 09.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:In contrast to lytic phages, filamentous phages are assembled in the inner membrane and secreted across the bacterial envelope without killing the host. For assembly and extrusion of the phage across the host cell wall, filamentous phages code for membrane-embedded morphogenesis proteins. In the outer membrane of Escherichia coli, the protein gp4 forms a pore-like structure, while gp1 and gp11 form a complex in the inner membrane of the host. By comparing sequences with other filamentous phages, we identified putative Walker A and B motifs in gp1 with a conserved lysine in the Walker A motif (K14), and a glutamic and aspartic acid in the Walker B motif (D88, E89). In this work we demonstrate that both, Walker A and Walker B, are essential for phage production. The crucial role of these key residues suggests that gp1 might be a molecular motor driving phage assembly. We further identified essential residues for the function of the assembly complex. Mutations in three out of six cysteine residues abolish phage production. Similarly, two out of six conserved glycine residues are crucial for gp1 function. We hypothesise that the residues represent molecular hinges allowing domain movement for nucleotide binding and phage assembly.
[Mh] Termos MeSH primário: Bacteriófago M13/genética
Bacteriófago M13/fisiologia
Inovirus/genética
Inovirus/fisiologia
Proteínas Virais/genética
Proteínas Virais/metabolismo
Montagem de Vírus
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Bacteriófago M13/química
Sequência Conservada
Análise Mutacional de DNA
Escherichia coli/metabolismo
Escherichia coli/virologia
Inovirus/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE


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[PMID]:27795361
[Au] Autor:Secor PR; Michaels LA; Smigiel KS; Rohani MG; Jennings LK; Hisert KB; Arrigoni A; Braun KR; Birkland TP; Lai Y; Hallstrand TS; Bollyky PL; Singh PK; Parks WC
[Ad] Endereço:Department of Microbiology, University of Washington, Seattle, Washington, USA psecor@uw.edu.
[Ti] Título:Filamentous Bacteriophage Produced by Pseudomonas aeruginosa Alters the Inflammatory Response and Promotes Noninvasive Infection In Vivo.
[So] Source:Infect Immun;85(1), 2017 Jan.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pseudomonas aeruginosa is an important opportunistic human pathogen that lives in biofilm-like cell aggregates at sites of chronic infection, such as those that occur in the lungs of patients with cystic fibrosis and nonhealing ulcers. During growth in a biofilm, P. aeruginosa dramatically increases the production of filamentous Pf bacteriophage (Pf phage). Previous work indicated that when in vivo Pf phage production was inhibited, P. aeruginosa was less virulent. However, it is not clear how the production of abundant quantities of Pf phage similar to those produced by biofilms under in vitro conditions affects pathogenesis. Here, using a murine pneumonia model, we show that the production of biofilm-relevant amounts of Pf phage prevents the dissemination of P. aeruginosa from the lung. Furthermore, filamentous phage promoted bacterial adhesion to mucin and inhibited bacterial invasion of airway epithelial cultures, suggesting that Pf phage traps P. aeruginosa within the lung. The in vivo production of Pf phage was also associated with reduced lung injury, reduced neutrophil recruitment, and lower cytokine levels. Additionally, when producing Pf phage, P. aeruginosa was less prone to phagocytosis by macrophages than bacteria not producing Pf phage. Collectively, these data suggest that filamentous Pf phage alters the progression of the inflammatory response and promotes phenotypes typically associated with chronic infection.
[Mh] Termos MeSH primário: Inflamação/microbiologia
Inflamação/virologia
Inovirus/crescimento & desenvolvimento
Infecções por Pseudomonas/microbiologia
Infecções por Pseudomonas/virologia
Pseudomonas aeruginosa/virologia
[Mh] Termos MeSH secundário: Animais
Biofilmes/crescimento & desenvolvimento
Fibrose Cística/microbiologia
Fibrose Cística/virologia
Pulmão/microbiologia
Pulmão/virologia
Macrófagos/microbiologia
Macrófagos/virologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Fagocitose/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE


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[PMID]:27743252
[Au] Autor:Yeh TY
[Ad] Endereço:Agricultural Biotechnology Laboratory, Plant Health Division, Auxergen Inc., 1100 Wicomico Street, Suite 700, Baltimore, MD, 21230, USA. yehty@auxergen.com.
[Ti] Título:Complete nucleotide sequence of a new filamentous phage, Xf109, which integrates its genome into the chromosomal DNA of Xanthomonas oryzae.
[So] Source:Arch Virol;162(2):567-572, 2017 Feb.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Unlike Ff-like coliphages, certain filamentous Inoviridae phages integrate their genomes into the host chromosome and enter a prophage state in their infectious cycle. This lysogenic life cycle was first reported for Xanthomonas citri Cf phage. However, except for the X. citri phages Cf and XacF1, complete genome sequence information about lysogenic Xanthomonas phages is not available to date. A proviral sequence of Xf109 phage was identified in the genome of Xanthomonas oryzae, the rice bacterial blight pathogen, and revived as infectious virions to lysogenize its host de novo. The genome of Xf109 phage is 7190 nucleotides in size and contains 12 predicted open reading frames in an organization similar to that of the Cf phage genome. Seven of the Xf109 proteins show significant sequence similarity to Cf and XacF1 phage proteins, while its ORF4 shares 92 % identity with the major coat protein of X. phage oryzae Xf. Integration of Xf109 phage DNA into the host genome is site-specific, and the attP/attB sequence contains the dif core sequence 5'-TATACATTATGCGAA-3', which is identical to that of Cf, XacF1, and Xanthomonas campestris phage Ï•Lf. To my knowledge, this is the first complete genome sequence of a filamentous bacteriophage that infects X. oryzae.
[Mh] Termos MeSH primário: Cromossomos Bacterianos/química
DNA Viral/genética
Genoma Viral
Inovirus/genética
Mutagênese Insercional
Xanthomonas/virologia
[Mh] Termos MeSH secundário: Sequência de Bases
Proteínas do Capsídeo/genética
Mapeamento Cromossômico
Expressão Gênica
Inovirus/isolamento & purificação
Lisogenia
Fases de Leitura Aberta
Oryza/microbiologia
Alinhamento de Sequência
Vírion/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (DNA, Viral)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170214
[Lr] Data última revisão:
170214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161016
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3105-3


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[PMID]:27645387
[Au] Autor:Renda BA; Chan C; Parent KN; Barrick JE
[Ad] Endereço:Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Center for Systems and Synthetic Biology, Center for Computational Biology and Bioinformatics, The University of Texas at Austin, Austin, Texas, USA.
[Ti] Título:Emergence of a Competence-Reducing Filamentous Phage from the Genome of Acinetobacter baylyi ADP1.
[So] Source:J Bacteriol;198(23):3209-3219, 2016 Dec 01.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial genomes commonly contain prophage sequences as a result of past infections with lysogenic phages. Many of these integrated viral sequences are believed to be cryptic, but prophage genes are sometimes coopted by the host, and some prophages may be reactivated to form infectious particles when cells are stressed or mutate. We found that a previously uncharacterized filamentous phage emerged from the genome of Acinetobacter baylyi ADP1 during a laboratory evolution experiment. This phage has a genetic organization similar to that of the Vibrio cholerae CTXÏ• phage. The emergence of the ADP1 phage was associated with the evolution of reduced transformability in our experimental populations, so we named it the competence-reducing acinetobacter phage (CRAÏ•). Knocking out ADP1 genes required for competence leads to resistance to CRAÏ• infection. Although filamentous bacteriophages are known to target type IV pili, this is the first report of a phage that apparently uses a competence pilus as a receptor. A. baylyi may be especially susceptible to this route of infection because every cell is competent during normal growth, whereas competence is induced only under certain environmental conditions or in a subpopulation of cells in other bacterial species. It is possible that CRAÏ•-like phages restrict horizontal gene transfer in nature by inhibiting the growth of naturally transformable strains. We also found that prophages with homology to CRAÏ• exist in several strains of Acinetobacter baumannii These CRAÏ•-like A. baumannii prophages encode toxins similar to CTXÏ• that might contribute to the virulence of this opportunistic multidrug-resistant pathogen. IMPORTANCE: We observed the emergence of a novel filamentous phage (CRAÏ•) from the genome of Acinetobacter baylyi ADP1 during a long-term laboratory evolution experiment. CRAÏ• is the first bacteriophage reported to require the molecular machinery involved in the uptake of environmental DNA for infection. Reactivation and evolution of CRAÏ• reduced the potential for horizontal transfer of genes via natural transformation in our experiment. Risk of infection by similar phages may limit the expression and maintenance of bacterial competence in nature. The closest studied relative of CRAÏ• is the Vibrio cholerae CTXÏ• phage. Variants of CRAÏ• are found in the genomes of Acinetobacter baumannii strains, and it is possible that phage-encoded toxins contribute to the virulence of this opportunistic multidrug-resistant pathogen.
[Mh] Termos MeSH primário: Acinetobacter baumannii/virologia
Inovirus/isolamento & purificação
Prófagos/isolamento & purificação
[Mh] Termos MeSH secundário: Acinetobacter baumannii/genética
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Genoma Bacteriano
Genoma Viral
Inovirus/classificação
Inovirus/genética
Inovirus/fisiologia
Prófagos/classificação
Prófagos/genética
Prófagos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE


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[PMID]:26939573
[Au] Autor:Piekarowicz A; Klyz A; Majchrzak M; Stein DC
[Ad] Endereço:Department of Virology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
[Ti] Título:Oral Immunization of Rabbits with S. enterica Typhimurium Expressing Neisseria gonorrhoeae Filamentous Phage Φ6 Induces Bactericidal Antibodies Against N. gonorrhoeae.
[So] Source:Sci Rep;6:22549, 2016 Mar 04.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:All Neisseria gonorrhoeae strains whose DNA sequences have been determined possess filamentous phage DNA sequences. To ascertain if phage encoded proteins could form the basis of a gonococcal vaccine, rabbits were orally infected with S. enterica Typhimurium strain χ3987 harboring phagemid NgoΦ6 fm. The elicited sera contained large quantities of anti-phage IgG and IgA antibodies that bound to the surface of N. gonorrhoeae cells, as shown by indirect fluorescent analysis and flow cytometry. The elicited sera was able to bind to several phage proteins. The sera also had bactericidal activity. These data demonstrate that N. gonorrhoeae filamentous phage can induce antibodies with anti-gonococcal activity and that phage proteins may be a candidate for vaccine development.
[Mh] Termos MeSH primário: Anticorpos Antibacterianos/biossíntese
Vacinas Bacterianas/imunologia
Bacteriófago phi 6/genética
Gonorreia/prevenção & controle
Inovirus/genética
Neisseria gonorrhoeae/virologia
Salmonella typhi/imunologia
[Mh] Termos MeSH secundário: Administração Oral
Animais
Anticorpos Antibacterianos/sangue
Bacteriólise/genética
Gonorreia/imunologia
Seres Humanos
Imunização
Neisseria gonorrhoeae/imunologia
Coelhos
Salmonella typhi/genética
Salmonella typhi/virologia
Ensaios de Anticorpos Bactericidas Séricos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Bacterial Vaccines)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160305
[St] Status:MEDLINE
[do] DOI:10.1038/srep22549


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[PMID]:26929122
[Au] Autor:Jian H; Xiong L; Xu G; Xiao X
[Ad] Endereço:State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology.
[Ti] Título:Filamentous phage SW1 is active and influences the transcriptome of the host at high-pressure and low-temperature.
[So] Source:Environ Microbiol Rep;8(3):358-62, 2016 06.
[Is] ISSN:1758-2229
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As the most abundant biological entities on the planet, viruses are involved in global biogeochemical cycles, and they have been shown to play an important role in the overall functioning of the deep-sea ecosystem. Nevertheless, little is known about whether and how deep-sea viruses affect the physiology of their bacterial hosts. Previously, the filamentous phage SW1 was identified in the bathypelagic bacterium Shewanella piezotolerans WP3, which was isolated from the upper sediment of West Pacific ocean. In this study, phage SW1 was shown to be active under in situ environmental conditions (20 MPa and 4°C) by transmission electron microscopy and reverse-transcription quantitative polymerase chain reaction. Further comparative analysis showed that SW1 had a significant influence on the growth and transcriptome of its host. The transcription of genes responsible for basic cellular activities, including the transcriptional/translational apparatus, arginine synthesis, purine metabolism and the flagellar motor, were down-regulated by the phage. Our results present the first characterization of a phage-host interaction under high-pressure and low-temperature conditions, which indicated that the phage adjusted the energy utilization strategy of the host for improved survival in deep-sea environments.
[Mh] Termos MeSH primário: Temperatura Baixa
Perfilação da Expressão Gênica
Pressão Hidrostática
Inovirus/crescimento & desenvolvimento
Shewanella/genética
Shewanella/virologia
[Mh] Termos MeSH secundário: Sedimentos Geológicos
Interações Hospedeiro-Parasita
Microscopia Eletrônica de Transmissão
Oceano Pacífico
Reação em Cadeia da Polimerase em Tempo Real
Shewanella/crescimento & desenvolvimento
Shewanella/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE
[do] DOI:10.1111/1758-2229.12388


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[PMID]:26908079
[Au] Autor:Luzar J; Molek P; Silar M; Korosec P; Kosnik M; Strukelj B; Lunder M
[Ad] Endereço:Faculty of Pharmacy, University of Ljubljana, Slovenia.
[Ti] Título:Identification and characterization of major cat allergen Fel d 1 mimotopes on filamentous phage carriers.
[So] Source:Mol Immunol;71:176-83, 2016 Mar.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cat allergy is one of the most prevalent allergies worldwide and can lead to the development of rhinitis and asthma. Thus far, only allergen extracts from natural sources have been used for allergen-specific immunotherapy. However, extracts and whole allergens in immunotherapy present an anaphylaxis risk. Identification of allergen epitopes or mimotopes has an important role in development of safe and effective allergen-specific immunotherapy. Moreover, with a suitable immunogenic carrier, the absence of sufficient immune response elicited by short peptides could be surmounted. In this study, we identified five structural mimotopes of the major cat allergen Fel d 1 by immunoscreening with random peptide phage libraries. The mimotopes were computationally mapped to the allergen surface, and their IgE reactivity was confirmed using sera from cat-allergic patients. Importantly, the mimotopes showed no basophil activation of the corresponding cat-allergic patients, which makes them good candidates for the development of hypoallergenic vaccine. As bacteriophage particles are becoming increasingly recognized as immunogenic carriers, we constructed bacteriophage particles displaying multiple copies of each selected mimotope on major phage coat protein. These constructed phages elicited T cell-mediated immune response, which was predominated by the type 1 T cell response. Mimotopes alone contributed to the type 1 T cell response by promoting IL-2 production. Fel d 1 mimotopes, as well as their filamentous phage immunogenic carriers, represent promising candidates in the development of hypoallergenic vaccine against cat allergy.
[Mh] Termos MeSH primário: Dessensibilização Imunológica/métodos
Glicoproteínas/imunologia
Hipersensibilidade/prevenção & controle
[Mh] Termos MeSH secundário: Adulto
Alérgenos/imunologia
Animais
Teste de Degranulação Basófila
Western Blotting
Gatos
Ensaio de Imunoadsorção Enzimática
Epitopos/imunologia
Vetores Genéticos
Seres Humanos
Imunoensaio
Inovirus
Mimetismo Molecular
Biblioteca de Peptídeos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Epitopes); 0 (Glycoproteins); 0 (Peptide Library); G408EE88II (Fel d 1 protein, Felis domesticus)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE


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[PMID]:26898180
[Au] Autor:Jian H; Xiong L; Xu G; Xiao X; Wang F
[Ad] Endereço:State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
[Ti] Título:Long 5' untranslated regions regulate the RNA stability of the deep-sea filamentous phage SW1.
[So] Source:Sci Rep;6:21908, 2016 Feb 22.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Virus production in the deep-sea environment has been found to be high, and viruses have been suggested to play significant roles in the overall functioning of this ecosystem. Nevertheless, little is known about these viruses, including the mechanisms that control their production, which makes them one of the least understood biological entities on Earth. Previously, we isolated the filamentous phage SW1, whose virus production and gene transcription were found to be active at low temperatures, from a deep-sea bacterium, Shewanella piezotolerans WP3. In this study, the operon structure of phage SW1 is presented, which shows two operons with exceptionally long 5' and 3' untranslated regions (UTRs). In addition, the 5'UTR was confirmed to significantly influence the RNA stability of the SW1 transcripts. Our study revealed novel regulation of the operon and led us to propose a unique regulatory mechanism for Inoviruses. This type of RNA-based regulation may represent a mechanism for significant viral production in the cold deep biosphere.
[Mh] Termos MeSH primário: Inovirus/genética
RNA Viral/genética
Shewanella/virologia
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas
Sequência de Bases
Regulação Viral da Expressão Gênica
Genes Virais
Óperon
Regiões Promotoras Genéticas
Ligação Proteica
Estabilidade de RNA
RNA Viral/metabolismo
Análise de Sequência de RNA
Shewanella/genética
Proteínas Virais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160223
[St] Status:MEDLINE
[do] DOI:10.1038/srep21908


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[PMID]:26602366
[Au] Autor:Meyer J; Brissac T; Frapy E; Omer H; Euphrasie D; Bonavita A; Nassif X; Bille E
[Ad] Endereço:1​ Institut Necker-Enfants Malades, 14 Rue Maria Helena Vieira Da Silva, CS 61431, 75014, Paris, France 4​ CNRS UMR 8253, Paris, France 3​ INSERM U1151, Paris, France 2​ Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
[Ti] Título:Characterization of MDAΦ, a temperate filamentous bacteriophage of Neisseria meningitidis.
[So] Source:Microbiology;162(2):268-82, 2016 Feb.
[Is] ISSN:1465-2080
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The mechanism by which Neisseria meningitidis becomes invasive is not well understood. Comparative genomics identified the presence of an 8 kb island in strains belonging to invasive clonal complexes. This island was designated MDA for meningococcal disease associated. MDA is highly conserved among meningococcal isolates and its analysis revealed a genomic organization similar to that of a filamentous prophage such as CTXΦ of Vibrio cholerae. Subsequent molecular investigations showed that the MDA island has indeed the characteristics of a filamentous prophage, which can enter into a productive cycle and is secreted using the type IV pilus (tfp) secretin PilQ. At least three genes of the prophage are necessary for the formation of the replicative cytoplasmic form (orf1, orf2 and orf9). Immunolabelling of the phage with antibodies against the major capsid protein, ORF4, confirmed that filamentous particles, about 1200 nm long, covered with ORF4 are present at the bacterial surface forming bundles in some places and interacting with pili. The MDA bacteriophage is able to infect different N. meningitidis strains, using the type IV pili as a receptor via an interaction with the adsorption protein ORF6. Altogether, these data demonstrate that the MDA island encodes a functional prophage able to produce infectious filamentous phage particles.
[Mh] Termos MeSH primário: Sítios de Ligação Microbiológicos/genética
Inovirus/genética
Neisseria meningitidis/genética
Neisseria meningitidis/virologia
Prófagos/genética
Receptores Virais/genética
[Mh] Termos MeSH secundário: Sequência de Bases
DNA Viral/genética
Fímbrias Bacterianas/virologia
Infecções Meningocócicas/microbiologia
Neisseria meningitidis/patogenicidade
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Receptors, Virus)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151126
[St] Status:MEDLINE
[do] DOI:10.1099/mic.0.000215



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