Base de dados : MEDLINE
Pesquisa : B04.123.691 [Categoria DeCS]
Referências encontradas : 243 [refinar]
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[PMID]:27771874
[Au] Autor:Fauvel B; Gantzer C; Cauchie HM; Ogorzaly L
[Ad] Endereço:Department of Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), 41, rue du Brill, 4422, Belvaux, Luxembourg.
[Ti] Título:In Situ Dynamics of F-Specific RNA Bacteriophages in a Small River: New Way to Assess Viral Propagation in Water Quality Studies.
[So] Source:Food Environ Virol;9(1):89-102, 2017 03.
[Is] ISSN:1867-0342
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The occurrence and propagation of enteric viruses in rivers constitute a major public health issue. However, little information is available on the in situ transport and spread of viruses in surface water. In this study, an original in situ experimental approach using the residence time of the river water mass was developed to accurately follow the propagation of F-specific RNA bacteriophages (FRNAPHs) along a 3-km studied river. Surface water and sediment of 9 sampling campaigns were collected and analyzed using both infectivity and RT-qPCR assays. In parallel, some physico-chemical variables such as flow rate, water temperature, conductivity and total suspended solids were measured to investigate the impact of environmental conditions on phage propagation. For campaigns with low flow rate and high temperature, the results highlight a decrease of infectious phage concentration along the river, which was successfully modelled according to a first-order negative exponential decay. The monitoring of infectious FRNAPHs belonging mainly to the genogroup II was confirmed with direct phage genotyping and total phage particle quantification. Reported k decay coefficients according to exponential models allowed for the determination of the actual in situ distance and time necessary for removing 90 % of infectious phage particles. This present work provides a new way to assess the true in situ viral propagation along a small river. These findings can be highly useful in water quality and risk assessment studies to determine the viral contamination spread from a point contamination source to the nearest recreational areas.
[Mh] Termos MeSH primário: Fagos RNA/isolamento & purificação
Rios/virologia
[Mh] Termos MeSH secundário: Fagos RNA/classificação
Fagos RNA/genética
Rios/química
Temperatura Ambiente
Poluição da Água/análise
Qualidade da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171202
[Lr] Data última revisão:
171202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1007/s12560-016-9266-0


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[PMID]:28111107
[Au] Autor:Rumnieks J; Tars K
[Ad] Endereço:Biomedical Research and Study Center, Ratsupites 1, LV1067 Riga, Latvia.
[Ti] Título:Crystal Structure of the Maturation Protein from Bacteriophage Qß.
[So] Source:J Mol Biol;429(5):688-696, 2017 Mar 10.
[Is] ISSN:1089-8638
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Virions of the single-stranded RNA bacteriophages contain a single copy of the maturation protein, which is bound to the phage genome and is required for the infectivity of the particles. The maturation protein mediates the adsorption of the virion to bacterial pili and the subsequent release and penetration of the genome into the host cell. Here, we report a crystal structure of the maturation protein from bacteriophage Qß. The protein has a bent, highly asymmetric shape and spans 110Å in length. Apart from small local substructures, the overall fold of the maturation protein does not resemble that of other known proteins. The protein is organized in two distinct regions, an α-helical part with a four-helix core, and a ß stranded part that contains a seven-stranded sheet in the central part and a five-stranded sheet at the tip of the protein. The Qß maturation protein has two distinct, positively charged areas at opposite sides of the α-helical part, which are involved in genomic RNA binding. The maturation protein binds to each of the surrounding coat protein dimers in the capsid differently, and the interaction is considerably weaker compared to coat protein interdimer contacts. The coat protein- or RNA-binding residues are not preserved among different ssRNA phage maturation proteins; instead, the distal end of the α-helical part is the most evolutionarily conserved, suggesting the importance of this region for maintaining the functionality of the protein.
[Mh] Termos MeSH primário: Bacteriófagos/química
Proteínas do Capsídeo/química
Regulação Viral da Expressão Gênica
RNA Viral/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Bacteriófagos/genética
Proteínas do Capsídeo/genética
Clonagem Molecular
Microscopia Crioeletrônica
Conformação Proteica
Fagos RNA/química
Fagos RNA/genética
RNA Viral/genética
Vírion/química
Vírion/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Qbeta ribonucleic acid); 0 (RNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE


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[PMID]:28040176
[Au] Autor:Hartard C; Banas S; Rivet R; Boudaud N; Gantzer C
[Ad] Endereço:Université de Lorraine, LCPME (Laboratoire de Chimie Physique et Microbiologie pour l'Environnement), UMR 7564, Faculté de Pharmacie, Nancy F-54000, France; CNRS, LCPME, UMR 7564, Nancy F-54000, France; Institut Jean Barriol, Université de Lorraine, Faculté des Sciences et Technologies, VandÅ“uvre-l
[Ti] Título:Rapid and sensitive method to assess human viral pollution in shellfish using infectious F-specific RNA bacteriophages: Application to marketed products.
[So] Source:Food Microbiol;63:248-254, 2017 May.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:F-specific RNA bacteriophages (FRNAPH) have been used as indicators of environmental fecal pollution for many years. While FRNAPH subgroup I (FRNAPH-I) are not host specific, some FRNAPH-II and -III strains appear specific to human pollution. Because a close relationship has been observed between FRNAPH-II genome and human norovirus (NoV) in shellfish, and because FRNAPH infectivity can easily be investigated unlike that of NoV, the detection of human infectious FRNAPH could therefore provide a valuable tool for assessing viral risk. In this study, an integrated cell culture real-time RT-PCR method has been developed to investigate infectious FRNAPH subgroup prevalence in oysters. This rapid screening method appears more sensitive than E. coli or NoV genome detection, and allows an FRNAPH subgroup present in low concentrations (0.05 PFU/g of oyster) to be detected in the presence of another 1000 times more concentrated, without any dissection step. Its application to marketed oysters (n = 135) over a 1-year period has allowed to identify the winter peak classically described for NoV or FRNAPH accumulation. Infectious FRNAPH were detected in 34% of batches, and 7% were suspected of having a human origin. This approach may be helpful to evaluate oyster's depuration processes, based on an infectious viral parameter.
[Mh] Termos MeSH primário: Segurança de Produtos ao Consumidor
Ostreidae/virologia
Fagos RNA/genética
Fagos RNA/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real/métodos
Frutos do Mar/virologia
Microbiologia da Água
Poluição da Água
[Mh] Termos MeSH secundário: Animais
Poluição Ambiental
Escherichia coli/genética
Fezes/virologia
Seres Humanos
Limite de Detecção
Norovirus/genética
Fagos RNA/classificação
Estações do Ano
Sensibilidade e Especificidade
Ensaio de Placa Viral
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


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[PMID]:27829245
[Au] Autor:Pumpens P; Renhofa R; Dishlers A; Kozlovska T; Ose V; Pushko P; Tars K; Grens E; Bachmann MF
[Ad] Endereço:Latvian Biomedical Research and Study Centre, University of Latvia, Riga, Latvia.
[Ti] Título:The True Story and Advantages of RNA Phage Capsids as Nanotools.
[So] Source:Intervirology;59(2):74-110, 2016.
[Is] ISSN:1423-0100
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:RNA phages are often used as prototypes for modern recombinant virus-like particle (VLP) technologies. Icosahedral RNA phage VLPs can be formed from coat proteins (CPs) and are efficiently produced in bacteria and yeast. Both genetic fusion and chemical coupling have been successfully used for the production of numerous chimeras based on RNA phage VLPs. In this review, we describe advances in RNA phage VLP technology along with the history of the Leviviridae family, including its taxonomical organization, genomic structure, and important role in the development of molecular biology. Comparative 3D structures of different RNA phage VLPs are used to explain the level of VLP tolerance to foreign elements displayed on VLP surfaces. We also summarize data that demonstrate the ability of CPs to tolerate different organic (peptides, oligonucleotides, and carbohydrates) and inorganic (metal ions) compounds either chemically coupled or noncovalently added to the outer and/or inner surfaces of VLPs. Finally, we present lists of nanotechnological RNA phage VLP applications, such as experimental vaccines constructed by genetic fusion and chemical coupling methodologies, nanocontainers for targeted drug delivery, and bioimaging tools.
[Mh] Termos MeSH primário: Capsídeo
Fagos RNA
Vacinas de Partículas Semelhantes a Vírus/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas do Capsídeo/genética
Proteínas do Capsídeo/imunologia
Portadores de Fármacos/química
Sistemas de Liberação de Medicamentos
Leviviridae/classificação
Leviviridae/genética
Conformação Molecular
Nanotecnologia/métodos
Vacinas de Partículas Semelhantes a Vírus/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Drug Carriers); 0 (Vaccines, Virus-Like Particle)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE
[do] DOI:10.1159/000449503


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[PMID]:27422833
[Au] Autor:Hartard C; Banas S; Loutreul J; Rincé A; Benoit F; Boudaud N; Gantzer C
[Ad] Endereço:Université de Lorraine, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564, Faculté de Pharmacie, Nancy, France CNRS, LCPME, UMR 7564, Nancy, France Institut Jean Barriol, Université de Lorraine, Faculté des Sciences et Technologies, VandÅ“uvre-lès-Nancy, France.
[Ti] Título:Relevance of F-Specific RNA Bacteriophages in Assessing Human Norovirus Risk in Shellfish and Environmental Waters.
[So] Source:Appl Environ Microbiol;82(18):5709-19, 2016 Sep 15.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Human noroviruses (HuNoVs) are the main cause of shellfish-borne gastroenteritis outbreaks. In the absence of routine technical approaches allowing infectious particles to be detected, this viral pathogen is currently targeted by genome research, leading to difficult interpretations. In this study, we investigated the potential of F-specific RNA bacteriophages (FRNAPH) as fecal and viral contamination indicators in shellfish and water from a local harvesting area. FRNAPH were also used as microbial source tracking tools. Constraints imposed by detection limits are illustrated here by the detection of infectious FRNAPH in several samples in the absence of FRNAPH genomes. The opposite situation was also observed, likely explained by the persistence of the genomes being greater than infectivity. Similar considerations may be applied to HuNoVs, suggesting that HuNoV genome targeting is of limited relevance in assessing infectious risks. While FRNAPH did not provide any benefits compared to Escherichia coli as fecal pollution indicators in water, novel observations were made in shellfish: contrary to E. coli, a seasonal trend of infectious FRNAPH concentrations was observed. These concentrations were higher than those found in water, confirming bioaccumulation in shellfish. This study also underlines a relationship between the presence of HuNoV genomes and those of human-specific FRNAPH subgroup II (FRNAPH-II) in shellfish collected throughout Europe. Further research should be undertaken to evaluate FRNAPH potential as an indicator of the presence of infectious HuNoVs. To this end, shellfish involved in HuNoV-caused gastroenteritis outbreaks should be analyzed for the presence of infectious FRNAPH-II. IMPORTANCE: This work provides new data about the use of F-specific RNA phages (FRNAPH) as a tool for evaluating fecal or viral contamination, especially in shellfish. In our case study, FRNAPH did not provide any benefits compared to E. coli as fecal pollution indicators in water but were found to be very useful in shellfish. Their concentrations in shellfish were higher than those found in the surrounding water, confirming bioaccumulation. This study also underlines a relationship between the presence of human norovirus genomes (HuNoVs) and those of FRNAPH subgroup II (FRNAPH-II). Considering that the two virus types have similar behaviors and since FRNAPH infectivity can be investigated, the specific detection of infectious FRNAPH-II could be regarded as an indication of the presence of infectious HuNoVs. The contribution of infectious human FRNAPH targeting for assessing the viral risk associated with HuNoVs in shellfish should thus be investigated.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/epidemiologia
Doenças Transmitidas por Alimentos/epidemiologia
Modelos Biológicos
Norovirus/isolamento & purificação
Fagos RNA/isolamento & purificação
Frutos do Mar/virologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Animais
Infecções por Caliciviridae/virologia
Escherichia coli/virologia
Doenças Transmitidas por Alimentos/virologia
Seres Humanos
Medição de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160717
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01528-16


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[PMID]:27208125
[Au] Autor:Hata A; Hanamoto S; Shirasaka Y; Yamashita N; Tanaka H
[Ad] Endereço:Research Center for Environmental Quality Management, Kyoto University, Otsu, Shiga, JapanPennsylvania State University hata.akihiko.33c@st.kyoto-u.ac.jp.
[Ti] Título:Quantitative Distribution of Infectious F-Specific RNA Phage Genotypes in Surface Waters.
[So] Source:Appl Environ Microbiol;82(14):4244-52, 2016 Jul 15.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: F-specific RNA phages (FRNAPHs) are considered potential viral indicators of water pollution due to their occurrence and stability in water environments. However, their suitability as viral indicators is not fully elucidated because the characteristics of FRNAPHs are variable depending on the genotype. In this study, for the characterization of infectious FRNAPH genotypes, integrated culture reverse transcription-PCR coupled with the most probable number approach was applied to surface water samples. Further, to recover low concentrations of FRNAPH genotypes, an FRNAPH recovery method was developed. The novel FRNAPH recovery method using a noncharged microfiltration membrane could effectively recover FRNAPH strains without inactivation, while a method using an electronegative microfiltration membrane resulted in the inactivation of some strains. Infectious FRNAPH genotypes in surface water samples were successfully quantified with an efficiency comparable to that of the conventional plaque assay. Genotype I (GI) and GII FRNAPHs tended to be predominant at locations impacted by treated and untreated municipal wastewater, respectively. The numbers and proportions of infectious FRNAPHs tended to be higher during the winter season when water temperature decreased. IMPORTANCE: Properties of FRNAPHs are highly variable depending on their genotypes. Previous typing methods for FRNAPHs are not quantitative and/or are based on molecular assays, which cannot differentiate infective strains from inactive strains. Due to the reasons mentioned above, the utility of FRNAPHs as viral indicators of water pollution has not been fully validated. In this study, a quantitative genotyping method for infectious FRNAPHs was developed and applied to surface water samples. The method enabled characterization of infectious FRNAPH genotypes in terms of their occurrence and seasonality. Moreover, comparison of the method to a conventional molecular assay (reverse transcription-quantitative PCR) enabled characterization of their stability. Our approach can provide novel findings for further validation of FRNAPHs as viral indicators of water pollution.
[Mh] Termos MeSH primário: Genótipo
Fagos RNA/classificação
Fagos RNA/isolamento & purificação
Carga Viral/métodos
Microbiologia da Água
[Mh] Termos MeSH secundário: Fagos RNA/genética
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160522
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.00621-16


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[PMID]:27110815
[Au] Autor:Leskinen K; Blasdel BG; Lavigne R; Skurnik M
[Ad] Endereço:Department of Bacteriology and Immunology, Medicum, and Research Programs Unit, Immunobiology, University of Helsinki, P.O.Box 21 (Haartmaninkatu 3), FIN-00014 HY Helsinki, Finland. katarzyna.leskinen@helsinki.fi.
[Ti] Título:RNA-Sequencing Reveals the Progression of Phage-Host Interactions between φR1-37 and Yersinia enterocolitica.
[So] Source:Viruses;8(4):111, 2016 Apr 22.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Despite the expanding interest in bacterial viruses (bacteriophages), insights into the intracellular development of bacteriophage and its impact on bacterial physiology are still scarce. Here we investigate during lytic infection the whole-genome transcription of the giant phage vB_YecM_φR1-37 (φR1-37) and its host, the gastroenteritis causing bacterium Yersinia enterocolitica. RNA sequencing reveals that the gene expression of φR1-37 does not follow a pattern typical observed in other lytic bacteriophages, as only selected genes could be classified as typically early, middle or late genes. The majority of the genes appear to be expressed constitutively throughout infection. Additionally, our study demonstrates that transcription occurs mainly from the positive strand, while the negative strand encodes only genes with low to medium expression levels. Interestingly, we also detected the presence of antisense RNA species, as well as one non-coding intragenic RNA species. Gene expression in the phage-infected cell is characterized by the broad replacement of host transcripts with phage transcripts. However, the host response in the late phase of infection was also characterized by up-regulation of several specific bacterial gene products known to be involved in stress response and membrane stability, including the Cpx pathway regulators, ATP-binding cassette (ABC) transporters, phage- and cold-shock proteins.
[Mh] Termos MeSH primário: Interações Hospedeiro-Patógeno
Fagos RNA/fisiologia
Yersinia enterocolitica/virologia
[Mh] Termos MeSH secundário: Perfilação da Expressão Gênica
Regulação Bacteriana da Expressão Gênica
Regulação Viral da Expressão Gênica
Genoma Viral
RNA não Traduzido
RNA Viral
Sequências Reguladoras de Ácido Ribonucleico
Análise de Sequência de RNA
Transcriptoma
Yersinia enterocolitica/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Untranslated); 0 (RNA, Viral); 0 (Regulatory Sequences, Ribonucleic Acid)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160426
[St] Status:MEDLINE


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[PMID]:27089359
[Au] Autor:Guo Y; Guo R; Zhou Q; Sun C; Zhang X; Liu Y; Liu Q
[Ad] Endereço:Dermatology and Venereology Department, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, China. lvninle@sina.com.
[Ti] Título:Chlamydiaphage φCPG1 Capsid Protein Vp1 Inhibits Chlamydia trachomatis Growth via the Mitogen-Activated Protein Kinase Pathway.
[So] Source:Viruses;8(4):99, 2016 Apr 14.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Chlamydia trachomatis is the most common cause of curable bacterial sexually transmitted infections worldwide. Although the pathogen is well established, the pathogenic mechanisms remain unclear. Given the current challenges of antibiotic resistance and blocked processes of vaccine development, the use of a specific chlamydiaphage may be a new treatment solution. φCPG1 is a lytic phage specific for Chlamydia caviae, and shows over 90% nucleotide sequence identity with other chlamydiaphages. Vp1 is the major capsid protein of φCPG1. Purified Vp1 was previously confirmed to inhibit Chlamydia trachomatis growth. We here report the first attempt at exploring the relationship between Vp1-treated C. trachomatis and the protein and gene levels of the mitogen-activated/extracellular regulated protein kinase (MAPK/ERK) pathway by Western blotting and real-time PCR, respectively. Moreover, we evaluated the levels of pro-inflammatory cytokines interleukin (IL)-8 and IL-1 by enzyme-linked immunosorbent assay after Vp1 treatment. After 48 h of incubation, the p-ERK level of the Vp1-treated group decreased compared with that of the Chlamydia infection group. Accordingly, ERK1 and ERK2 mRNA expression levels of the Vp1-treated group also decreased compared with the Chlamydia infection group. IL-8 and IL-1 levels were also decreased after Vp1 treatment compared with the untreated group. Our results demonstrate that the inhibition effect of the chlamydiaphage φCPG1 capsid protein Vp1 on C. trachomatis is associated with the MAPK pathway, and inhibits production of the pro-inflammatory cytokines IL-8 and IL-1. The bacteriophages may provide insight into a new signaling transduction mechanism to influence their hosts, in addition to bacteriolysis.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/metabolismo
Infecções por Chlamydia/metabolismo
Infecções por Chlamydia/microbiologia
Chlamydia trachomatis/virologia
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Fagos RNA/fisiologia
Transdução de Sinais
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Azitromicina/farmacologia
Linhagem Celular
Chlamydia trachomatis/efeitos dos fármacos
Seres Humanos
Interleucina-1/metabolismo
Interleucina-8/metabolismo
Camundongos
Proteína Quinase 1 Ativada por Mitógeno/metabolismo
Proteína Quinase 3 Ativada por Mitógeno/metabolismo
Fosforilação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Capsid Proteins); 0 (Interleukin-1); 0 (Interleukin-8); 83905-01-5 (Azithromycin); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3); EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160419
[St] Status:MEDLINE


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[PMID]:27010970
[Au] Autor:Krishnamurthy SR; Janowski AB; Zhao G; Barouch D; Wang D
[Ad] Endereço:Departments of Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, United States of America.
[Ti] Título:Hyperexpansion of RNA Bacteriophage Diversity.
[So] Source:PLoS Biol;14(3):e1002409, 2016 Mar.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteriophage modulation of microbial populations impacts critical processes in ocean, soil, and animal ecosystems. However, the role of bacteriophages with RNA genomes (RNA bacteriophages) in these processes is poorly understood, in part because of the limited number of known RNA bacteriophage species. Here, we identify partial genome sequences of 122 RNA bacteriophage phylotypes that are highly divergent from each other and from previously described RNA bacteriophages. These novel RNA bacteriophage sequences were present in samples collected from a range of ecological niches worldwide, including invertebrates and extreme microbial sediment, demonstrating that they are more widely distributed than previously recognized. Genomic analyses of these novel bacteriophages yielded multiple novel genome organizations. Furthermore, one RNA bacteriophage was detected in the transcriptome of a pure culture of Streptomyces avermitilis, suggesting for the first time that the known tropism of RNA bacteriophages may include gram-positive bacteria. Finally, reverse transcription PCR (RT-PCR)-based screening for two specific RNA bacteriophages in stool samples from a longitudinal cohort of macaques suggested that they are generally acutely present rather than persistent.
[Mh] Termos MeSH primário: Variação Genética
Genoma Viral
Fagos RNA/genética
[Mh] Termos MeSH secundário: Metagenoma
Microbiota
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170316
[Lr] Data última revisão:
170316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160325
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.1002409


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[PMID]:26971808
[Au] Autor:Fauvel B; Cauchie HM; Gantzer C; Ogorzaly L
[Ad] Endereço:Luxembourg Institute of Science and Technology (LIST), Department of Environmental Research and Innovation (ERIN), 41, rue du Brill, L-4422 Belvaux, Luxembourg; Université de Lorraine, Laboratoire de Chimie, Physique et Microbiologie pour l'Environnement (LCPME), UMR 7564, Faculté de Pharmacie, Nanc
[Ti] Título:Contribution of hydrological data to the understanding of the spatio-temporal dynamics of F-specific RNA bacteriophages in river water during rainfall-runoff events.
[So] Source:Water Res;94:328-40, 2016 May 01.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Heavy rainfall events were previously reported to bring large amounts of microorganisms in surface water, including viruses. However, little information is available on the origin and transport of viral particles in water during such rain events. In this study, an integrative approach combining microbiological and hydrological measurements was investigated to appreciate the dynamics and origins of F-specific RNA bacteriophage fluxes during two distinct rainfall-runoff events. A high frequency sampling (automatic sampler) was set up to monitor the F-specific RNA bacteriophages fluxes at a fine temporal scale during the whole course of the rainfall-runoff events. A total of 276 rainfall-runoff samples were collected and analysed using both infectivity and RT-qPCR assays. The results highlight an increase of 2.5 log10 and 1.8 log10 of infectious F-specific RNA bacteriophage fluxes in parallel of an increase of the water flow levels for both events. Faecal pollution was characterised as being mainly from anthropic origin with a significant flux of phage particles belonging to the genogroup II. At the temporal scale, two successive distinct waves of phage pollution were established and identified through the hydrological measurements. The first arrival of phages in the water column was likely to be linked to the resuspension of riverbed sediments that was responsible for a high input of genogroup II. Surface runoff contributed further to the second input of phages, and more particularly of genogroup I. In addition, an important contribution of infectious phage particles has been highlighted. These findings imply the existence of a close relationship between the risk for human health and the viral contamination of flood water.
[Mh] Termos MeSH primário: Fagos RNA/isolamento & purificação
Chuvas
Rios/virologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Monitoramento Ambiental
Fezes/virologia
Sedimentos Geológicos/virologia
Hidrologia
Luxemburgo
Fagos RNA/classificação
Análise Espaço-Temporal
Movimentos da Água
Poluentes da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Water Pollutants)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160315
[St] Status:MEDLINE



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