Base de dados : MEDLINE
Pesquisa : B04.123.831 [Categoria DeCS]
Referências encontradas : 1323 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 133 ir para página                         

  1 / 1323 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28800818
[Au] Autor:Chang Y; Kim M; Ryu S
[Ad] Endereço:Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
[Ti] Título:Characterization of a novel endolysin LysSA11 and its utility as a potent biocontrol agent against Staphylococcus aureus on food and utensils.
[So] Source:Food Microbiol;68:112-120, 2017 Dec.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Here we show that the LysSA11 endolysin, derived from the virulent Staphylococcus aureus phage SA11, has lytic activity against staphylococcal strains. Bioinformatics analysis revealed an enzymatically active CHAP (cysteine, histidine-dependent amidohydrolases/peptidases) domain at the N-terminus of LysSA11 that showed amidase activity. A novel cell wall binding domain (CBD) in the C-terminus could bind to a broad spectrum of staphylococcal cells. The bactericidal activity of LysSA11 was determined in food and utensils artificially contaminated with methicillin-resistant S. aureus (MRSA). The amounts of MRSA bacteria in milk and on ham were significantly reduced by 1.44-log CFU/mL and 3.12-log CFU/cm , respectively, within 15 min at refrigeration temperature (4 °C) and by 2.02-log CFU/mL and 3.37-log CFU/cm , respectively, within 15 min at room temperature (25 °C). Moreover, a polypropylene plastic cutting board and a stainless steel knife artificially contaminated with approximately 4-log CFU/cm of MRSA also showed complete bacterial elimination after a 30-min treatment with 1.35 µM of LysSA11. The data presented here strongly suggest that the novel CBD-containing staphylococcal endolysin LysSA11 can be used both as a food antimicrobial and as a practical sanitizer for utensils.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Endopeptidases/farmacologia
Fagos de Staphylococcus/química
Fagos de Staphylococcus/enzimologia
Staphylococcus aureus/efeitos dos fármacos
Proteínas Virais/farmacologia
[Mh] Termos MeSH secundário: Utensílios de Alimentação e Culinária
Endopeptidases/metabolismo
Microbiologia de Alimentos
Aço Inoxidável/análise
Staphylococcus aureus/crescimento & desenvolvimento
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Viral Proteins); 12597-68-1 (Stainless Steel); EC 3.4.- (Endopeptidases); EC 3.4.99.- (endolysin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170813
[St] Status:MEDLINE


  2 / 1323 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28730979
[Au] Autor:Uchiyama J; Taniguchi M; Kurokawa K; Takemura-Uchiyama I; Ujihara T; Shimakura H; Sakaguchi Y; Murakami H; Sakaguchi M; Matsuzaki S
[Ad] Endereço:1​School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
[Ti] Título:Adsorption of Staphylococcus viruses S13' and S24-1 on Staphylococcus aureus strains with different glycosidic linkage patterns of wall teichoic acids.
[So] Source:J Gen Virol;98(8):2171-2180, 2017 Aug.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The group of phages belonging to the family Podoviridae, genus P68virus, including Staphylococcus viruses S13' and S24-1, are important because of their benefits in phage therapy against Staphylococcus aureus infections. The O-glycosidic linkage patterns of wall teichoic acids (WTAs) in S. aureus cell walls seem to be important for adsorption of this phage group. In this study, the adsorption of Staphylococcus viruses S13' and S24-1 to S. aureus was examined using strains with modified WTA glycosidic linkage patterns. We found that the ß-O-N-acetylglucosamine of WTAs was essential for S13' adsorption, while N-acetylglucosamine, regardless of the α- and ß-O-glycosidic linkages of the WTAs, was essential for S24-1 adsorption. Next, examining the binding activities of their receptor-binding proteins (RBPs) to cell walls with different WTA glycosidic patterns, the ß-O-N-acetylglucosamine of the WTAs was essential for S13' RBP binding, while N-acetylglucosamine, regardless of the α- and ß-O-glycosidic linkages of the WTAs, was essential for S24-1 RBP binding. Therefore, the results of the RBP binding assays were consistent with those of the phage adsorption assays. Bioinformatic analysis suggested that the RBPs of Staphylococcus viruses S13' and S24-1 were structurally similar to the RBPs of phage phi11 of thefamily Siphoviridae. Phylogenetic analysis of the RBPs indicated that two phylogenetic subclusters in the family Podoviridae were related to the glycosidic linkage patterns required for phage adsorption, possibly mediated by RBPs. We hope that this study will encourage the future development of therapeutic phages.
[Mh] Termos MeSH primário: Receptores Virais/metabolismo
Fagos de Staphylococcus/fisiologia
Staphylococcus aureus/virologia
Ácidos Teicoicos/metabolismo
Ligação Viral
[Mh] Termos MeSH secundário: Podoviridae/fisiologia
Receptores Virais/química
Ácidos Teicoicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Virus); 0 (Teichoic Acids)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000865


  3 / 1323 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28324178
[Au] Autor:Senevirathne A; Ghosh K; Roh E; Kim KP
[Ad] Endereço:Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Jeollabuk-do, 561-756, Republic of Korea.
[Ti] Título:Complete genome sequence analysis of a novel Staphylococcus phage StAP1 and proposal of a new species in the genus Silviavirus.
[So] Source:Arch Virol;162(7):2145-2148, 2017 Jul.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bacteriophage StAP1 was isolated from a soil sample infecting Staphylococcus aureus and S. xylosus. Its genome was found to be 135,502 base pairs (bp) long with 30.00 mol% G+C content and 192 open reading frames. While no tRNA encoding genes were identified, 7 mobile elements were found to interrupt five StAP1 open reading frames. Comparative genomic and proteomic analysis consistently supports the establishment of a new species in the genus Silviavirus.
[Mh] Termos MeSH primário: Filogenia
Fagos de Staphylococcus/classificação
Staphylococcus aureus/virologia
[Mh] Termos MeSH secundário: Composição de Bases
Fases de Leitura Aberta
Proteômica
Análise de Sequência de DNA
Microbiologia do Solo
Fagos de Staphylococcus/genética
Fagos de Staphylococcus/ultraestrutura
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3316-2


  4 / 1323 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28286740
[Au] Autor:Drilling AJ; Ooi ML; Miljkovic D; James C; Speck P; Vreugde S; Clark J; Wormald PJ
[Ad] Endereço:Department of Surgery-Otolaryngology Head and Neck Surgery, The University of Adelaide Adelaide, SA, Australia.
[Ti] Título:Long-Term Safety of Topical Bacteriophage Application to the Frontal Sinus Region.
[So] Source:Front Cell Infect Microbiol;7:49, 2017.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:biofilms contribute negatively to a number of chronic conditions, including chronic rhinosinusitis (CRS). With the inherent tolerance of biofilm-bound bacteria to antibiotics and the global problem of bacterial antibiotic resistance, the need to develop novel therapeutics is paramount. Phage therapy has previously shown promise in treating sinonasal biofilms. This study investigates the long term (20 days) safety of topical sinonasal flushes with bacteriophage suspensions. The bacteriophage cocktail NOV012 against selected for this work contains two highly characterized and different phages, P68 and K710. Host range was assessed against strains isolated from CRS patients using agar spot tests. NOV012 was applied topically to the frontal sinus region of sheep, twice daily for 20 days. General sheep wellbeing, mucosal structural changes and inflammatory load were assessed to determine safety of NOV012 application. NOV012 could lyse 52/61 (85%) of a panel of locally derived CRS clinical isolates. Application of NOV012 to the frontal sinuses of sheep for 20 days was found to be safe, with no observed inflammatory infiltration or tissue damage within the sinus mucosa. NOV012 cocktail appears safe to apply for extended periods to sheep sinuses and it could infect and lyse a wide range of CRS clinical isolates. This indicates that phage therapy has strong potential as a treatment for chronic bacterial rhinosinusitis.
[Mh] Termos MeSH primário: Seio Frontal/microbiologia
Terapia por Fagos/efeitos adversos
Terapia por Fagos/métodos
Sinusite/terapia
[Mh] Termos MeSH secundário: Administração Tópica
Animais
Modelos Animais de Doenças
Especificidade de Hospedeiro
Mucosa Nasal/patologia
Ovinos
Fagos de Staphylococcus/fisiologia
Staphylococcus aureus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2017.00049


  5 / 1323 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28235706
[Au] Autor:Zhou Y; Zhang H; Bao H; Wang X; Wang R
[Ad] Endereço:Institute of Food Safety, Key Lab of Agro-Food Safety and Quality Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
[Ti] Título:The lytic activity of recombinant phage lysin LysKΔamidase against staphylococcal strains associated with bovine and human infections in the Jiangsu province of China.
[So] Source:Res Vet Sci;111:113-119, 2017 Apr.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We investigated the lytic activity of the bacteriophage endolysin (lysin) LysKΔamidase against live methicillin-resistant and-susceptible staphylococcal strains clinically isolated from bovine milk and humans from different origins of China. Antibiotic resistance patterns, multilocus sequence typing and SCCmec type of 137 staphylococcal strains isolated from bovine milk associated with bovine mastitis and human diseases were studied. A lytic enzyme, LysKΔamidase, was constructed by fusing the N-terminal 220 amino acids with the C-terminal 105 amino acids of staphylococcal phage lysin LysK. Herein, the antimicrobial activity of LysKΔamidase against 66 methicillin-resistant staphylococcal strains and 71 methicillin-susceptible staphylococcal strains isolated from bovine milk and from humans in China were studied. Our results show that the lysin displayed a broad lytic spectrum; in vitro treatment killed all 137 of the milk and clinical isolates of staphylococci strains tested, including MRSA, methicillin-susceptible S. aureus (MSSA), MR-Staphylococcus hominis ssp. homins, MR-Staphylococcus epidermidis and MR-Staphylococcus haemolyticus as evidenced by scanning electron microscopy, transmission electron microscopy, turbidity reduction assay and disruption of biofilms. The present results suggest that LysKΔamidase has the potential to be an alternative therapeutic agent against pathogenic methicillin-resistant and-susceptible staphylococcal strains isolated from China.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Enzimas/genética
Mastite Bovina/microbiologia
Infecções Estafilocócicas/veterinária
Fagos de Staphylococcus/genética
Staphylococcus aureus/virologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/tratamento farmacológico
Doenças dos Bovinos/microbiologia
China
Enzimas/metabolismo
Feminino
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/virologia
Leite/microbiologia
Infecções Estafilocócicas/tratamento farmacológico
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Enzymes); 0 (lysin, streptococcus bacteriophage)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE


  6 / 1323 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28230780
[Au] Autor:Borysowski J; Miedzybrodzki R; Wierzbicki P; Klosowska D; Korczak-Kowalska G; Weber-Dabrowska B; Górski A
[Ad] Endereço:Department of Clinical Immunology, Transplantation Institute, Medical University of Warsaw, Nowogrodzka Str. 59, 02-006 Warsaw, Poland. jborysowski@interia.pl.
[Ti] Título:A3R Phage and Staphylococcus aureus Lysate Do Not Induce Neutrophil Degranulation.
[So] Source:Viruses;9(2), 2017 Feb 21.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to evaluate the effects of A3R phage and lysate obtained after phage infection on neutrophil degranulation. The exocytosis of primary and secondary granules from neutrophils was investigated in vitro in whole blood specimens by flow cytometry based on the expression of specific markers of exocytosis (CD63 for primary granules and CD66b for secondary granules). We found that both A3R and lysate had no significant effect on the exocytosis of primary and secondary granules. These data suggest that neither A3R virions nor any products of phage-induced lysis of are likely to induce neutrophil degranulation in patients who are treated with phage preparations. Since neutrophil granules contain some potentially toxic proteins, our results provide an important argument for the safety of phage therapy. Moreover, these data indicate that the induction of neutrophil degranulation is not likely to contribute to antibacterial effects of phages.
[Mh] Termos MeSH primário: Bacteriólise
Degranulação Celular
Neutrófilos/imunologia
Fagos de Staphylococcus/imunologia
Staphylococcus aureus/imunologia
[Mh] Termos MeSH secundário: Adulto
Exocitose
Citometria de Fluxo
Voluntários Saudáveis
Seres Humanos
Terapia por Fagos/efeitos adversos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE


  7 / 1323 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28179010
[Au] Autor:Cui Z; Feng T; Gu F; Li Q; Dong K; Zhang Y; Zhu Y; Han L; Qin J; Guo X
[Ad] Endereço:Department of Immunology and Microbiology, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China. czl_phage@126.com.
[Ti] Título:Characterization and complete genome of the virulent Myoviridae phage JD007 active against a variety of Staphylococcus aureus isolates from different hospitals in Shanghai, China.
[So] Source:Virol J;14(1):26, 2017 Feb 08.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The implementation of phage therapy is re-emerging with the increase in widespread antibiotic-resistant bacteria. METHODS: Staphylococcus phage JD007 was characterized and its complete genome sequence analysed. RESULTS: Staphylococcus phage JD007 was classified as belonging to the Myoviridae family based on its morphology, as observed by transmission electron microscopy. Its lytic activity was stable between pH 5-11 and below 42 °C; moreover, an absorbance curve showed that nearly 90% of the viral particles had adsorbed to its host after a 20 min co-incubation. The complete genome size is 141,836 bp, making JD007 one of the largest Staphylococcus phages of Myoviridae. No identifiable resistance or virulence genes were found in the JD007 genome. JD007 was able to lyse 95% of S. aureus isolates, including the prevalent ST239-MRSA and ST59-MRSA strains isolated from different hospitals in Shanghai, China, and inhibition assays showed that JD007 could inhibit S. aureus growth at a multiplicity of infection of 0.1. CONCLUSIONS: The results suggested that Staphylococcus phage JD007 can potentially be used in phage therapy or for the detection of S. aureus.
[Mh] Termos MeSH primário: Genoma Viral
Especificidade de Hospedeiro
Myoviridae/genética
Myoviridae/fisiologia
Fagos de Staphylococcus/genética
Fagos de Staphylococcus/fisiologia
Staphylococcus aureus/virologia
[Mh] Termos MeSH secundário: China
Infecção Hospitalar/microbiologia
DNA Viral/química
DNA Viral/genética
Seres Humanos
Concentração de Íons de Hidrogênio
Viabilidade Microbiana/efeitos dos fármacos
Viabilidade Microbiana/efeitos da radiação
Microscopia Eletrônica de Transmissão
Myoviridae/classificação
Myoviridae/isolamento & purificação
Análise de Sequência de DNA
Infecções Estafilocócicas/microbiologia
Fagos de Staphylococcus/classificação
Fagos de Staphylococcus/isolamento & purificação
Staphylococcus aureus/isolamento & purificação
Temperatura Ambiente
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-017-0701-0


  8 / 1323 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27498125
[Au] Autor:Chang Y; Ryu S
[Ad] Endereço:Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, South Korea.
[Ti] Título:Characterization of a novel cell wall binding domain-containing Staphylococcus aureus endolysin LysSA97.
[So] Source:Appl Microbiol Biotechnol;101(1):147-158, 2017 Jan.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Endolysin from Staphylococcus aureus phage SA97 (LysSA97) was cloned and investigated. LysSA97 specifically lyse the staphylococcal strains and effectively disrupted staphylococcal biofilms. Bioinformatic analysis of LysSA97 revealed a novel putative cell wall binding domain (CBD) as well as two enzymatically active domains (EADs) containing cysteine, histidine-dependent amidohydrolases/peptidases (CHAP, PF05257) and N-acetylmuramoyl-L-alanine amidase (Amidase-3, PF01520) domains. Comparison of 98 endolysin genes of S. aureus phages deposited in GenBank showed that they can be classified into six groups based on their domain composition. Interestingly, approximately 80.61 % of the staphylococcal endolysins have a src-homology 3 (SH3, PF08460) domain as CBD, but the remaining 19.39 %, including LysSA97, has a putative C-terminal CBD with no homology to the known CBD. The fusion protein containing green fluorescent protein and the putative CBD of LysSA97 showed a specific binding spectrum against staphylococcal cells comparable to SH3 domain (PF08460), suggesting that the C-terminal domain of LysSA97 is a novel CBD of staphylococcal endolysins.
[Mh] Termos MeSH primário: Parede Celular/metabolismo
Endopeptidases/genética
Endopeptidases/metabolismo
Fagos de Staphylococcus/enzimologia
Fagos de Staphylococcus/genética
Staphylococcus aureus/virologia
[Mh] Termos MeSH secundário: Sítios de Ligação
Biofilmes/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Clonagem Molecular
Biologia Computacional
Ligação Proteica
Domínios Proteicos
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.4.- (Endopeptidases); EC 3.4.99.- (endolysin)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160808
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-016-7747-6


  9 / 1323 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27886033
[Au] Autor:Oduor JM; Onkoba N; Maloba F; Arodi WO; Nyachieo A
[Ad] Endereço:Institute of Primate Research (IPR), Nairobi, Kenya. josemislredo@gmail.com.
[Ti] Título:Efficacy of lytic Staphylococcus aureus bacteriophage against multidrug-resistant Staphylococcus aureus in mice.
[So] Source:J Infect Dev Ctries;10(11):1208-1213, 2016 Nov 24.
[Is] ISSN:1972-2680
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: The use of bacteriophages as an alternative treatment method against multidrug-resistant bacteria has not been explored in Kenya. This study sought to determine the efficacy of environmentally obtained lytic bacteriophage against multidrug-resistant Staphylococcus aureus (MDRSA) bacterium in mice. METHODOLOGY: Staphylococcus aureus bacterium and S. aureus-specific lytic phage were isolated from sewage and wastewater collected within Nairobi County, Kenya. Thirty mice were randomly assigned into three groups: MDRSA infection group (n = 20), phage-infection group (n = 5), and non-infection group (n = 5). The MDRSA infection group was further subdivided into three groups: clindamycin treatment (8 mg/kg; n = 5), lytic phage treatment (108 PFU/mL (n = 5), and a combination treatment of clindamycin and lytic phage (n = 5). Treatments were done at either 24 or 72 hours post-infection (p.i), and data on efficacy, bacterial load, and animal physical health were collected. RESULTS: Treatment with phage was more effective (100%) than with clindamycin (62.25% at 24 hours p.i and 87.5% at 72 hours p.i.) or combination treatment (75% at 24 hours p.i. and 90% at 72 hours p.i.) (p < 0.001). CONCLUSIONS: The results show that the environmentally obtained S. aureus lytic bacteriophage has therapeutic potential against MDRSA bacterium in mice.
[Mh] Termos MeSH primário: Terapia Biológica/métodos
Infecções Estafilocócicas/terapia
Fagos de Staphylococcus/crescimento & desenvolvimento
Staphylococcus aureus/virologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/administração & dosagem
Carga Bacteriana
Clindamicina/administração & dosagem
Terapia Combinada
Modelos Animais de Doenças
Farmacorresistência Bacteriana Múltipla
Feminino
Quênia
Masculino
Camundongos Endogâmicos BALB C
Infecções Estafilocócicas/patologia
Fagos de Staphylococcus/isolamento & purificação
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/isolamento & purificação
Análise de Sobrevida
Resultado do Tratamento
Carga Viral
Águas Residuais/microbiologia
Águas Residuais/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Waste Water); 3U02EL437C (Clindamycin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170203
[Lr] Data última revisão:
170203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE
[do] DOI:10.3855/jidc.7931


  10 / 1323 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27825477
[Au] Autor:Li Q; Xie X; Yin K; Tang Y; Zhou X; Chen Y; Xia J; Hu Y; Ingmer H; Li Y; Jiao X
[Ad] Endereço:Jiangsu Key Lab of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China; Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denm
[Ti] Título:Characterization of CRISPR-Cas system in clinical Staphylococcus epidermidis strains revealed its potential association with bacterial infection sites.
[So] Source:Microbiol Res;193:103-110, 2016 Dec.
[Is] ISSN:1618-0623
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Staphylococcus epidermidis is considered as a major cause of nosocomial infections, bringing an immense burden to healthcare systems. Virulent phages have been confirmed to be efficient in combating the pathogen, but the prensence of CRISPR-Cas system, which is a bacterial immune system eliminating phages was reported in few S. epidermidis strains. In this study, the CRISPR-Cas system was detected in 12 from almost 300 published genomes in GenBank and by PCR of cas6 gene in 18 strains out of 130 clinical isolates obtained in Copenhagen. Four strains isolated in 1965-1966 harboured CRISPR elements confirming that this immunity system was not recently acquired by S. epidermidis. In these CRISPR-positive strains, 44 and 12 spacers were found to belong to CRISPR1 and CRISPR2 elements, respectively. However, only 15 spacers displayed homology to reported phages and plasmids DNA. Interestingly, 5 different spacers located in the CRISPR1 locus with homolgy to virulent phage 6ec DNA sequences, and 19 strains each carrying 2 or 3 different spacers recognizing this phage, implied that the CRISPR-Cas immunity could be abrogated by nucleotide mismatch between the spacer and its target phage sequence, while new spacers obtained from the evolved phage could recover the CRISPR interference. In addition, phylogenetic analysis of the 29 CRISPR-positive isolates divided them into four lineages, with 81% human blood isolates as a distinct sub-lineage, suggesting that the CRISPR difference is closely related to diverse habitats. Knowledge of CRISPR and its prevalence may ultimately be applied in the understanding of origin and evolution of CRISPR-positive S. epidermidis strains.
[Mh] Termos MeSH primário: Sistemas CRISPR-Cas
Staphylococcus epidermidis/enzimologia
[Mh] Termos MeSH secundário: Infecções Bacterianas
Biologia Computacional
DNA Bacteriano/genética
Dinamarca
Seres Humanos
Plasmídeos
Reação em Cadeia da Polimerase
Infecções Estafilocócicas/microbiologia
Fagos de Staphylococcus/genética
Staphylococcus epidermidis/genética
Staphylococcus epidermidis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE



página 1 de 133 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde