Base de dados : MEDLINE
Pesquisa : B04.123.900 [Categoria DeCS]
Referências encontradas : 29 [refinar]
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  1 / 29 MEDLINE  
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[PMID]:27466389
[Au] Autor:Berjón-Otero M; Villar L; Salas M; Redrejo-Rodríguez M
[Ad] Endereço:Centro de Biología Molecular 'Severo Ochoa', Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid, Nicolás Cabrera, 1, Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.
[Ti] Título:Disclosing early steps of protein-primed genome replication of the Gram-positive tectivirus Bam35.
[So] Source:Nucleic Acids Res;44(20):9733-9744, 2016 Nov 16.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Protein-primed replication constitutes a generalized mechanism to initiate DNA or RNA synthesis in a number of linear genomes of viruses, linear plasmids and mobile elements. By this mechanism, a so-called terminal protein (TP) primes replication and becomes covalently linked to the genome ends. Bam35 belongs to a group of temperate tectiviruses infecting Gram-positive bacteria, predicted to replicate their genomes by a protein-primed mechanism. Here, we characterize Bam35 replication as an alternative model of protein-priming DNA replication. First, we analyze the role of the protein encoded by the ORF4 as the TP and characterize the replication mechanism of the viral genome (TP-DNA). Indeed, full-length Bam35 TP-DNA can be replicated using only the viral TP and DNA polymerase. We also show that DNA replication priming entails the TP deoxythymidylation at conserved tyrosine 194 and that this reaction is directed by the third base of the template strand. We have also identified the TP tyrosine 172 as an essential residue for the interaction with the viral DNA polymerase. Furthermore, the genetic information of the first nucleotides of the genome can be recovered by a novel single-nucleotide jumping-back mechanism. Given the similarities between genome inverted terminal repeats and the genes encoding the replication proteins, we propose that related tectivirus genomes can be replicated by a similar mechanism.
[Mh] Termos MeSH primário: Replicação do DNA
DNA Viral
Genoma Viral
Tectiviridae/fisiologia
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Fagos Bacilares/fisiologia
Sequência de Bases
Sítios de Ligação
Fases de Leitura Aberta/genética
Ligação Proteica
Proteínas Virais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160729
[St] Status:MEDLINE


  2 / 29 MEDLINE  
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[PMID]:25261525
[Au] Autor:Gillis A; Mahillon J
[Ad] Endereço:Laboratory of Food and Environmental Microbiology, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
[Ti] Título:Influence of lysogeny of Tectiviruses GIL01 and GIL16 on Bacillus thuringiensis growth, biofilm formation, and swarming motility.
[So] Source:Appl Environ Microbiol;80(24):7620-30, 2014 Dec.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacillus thuringiensis is an entomopathogenic bacterium that has been used as an efficient biopesticide worldwide. Despite the fact that this bacterium is usually described as an insect pathogen, its life cycle in the environment is still largely unknown. B. thuringiensis belongs to the Bacillus cereus group of bacteria, which has been associated with many mobile genetic elements, such as species-specific temperate or virulent bacteriophages (phages). Temperate (lysogenic) phages are able to establish a long-term relationship with their host, providing, in some cases, novel ecological traits to the bacterial lysogens. Therefore, this work focuses on evaluating the potential influence of temperate tectiviruses GIL01 and GIL16 on the development of different life traits of B. thuringiensis. For this purpose, a B. thuringiensis serovar israelensis plasmid-cured (nonlysogenic) strain was used to establish bacterial lysogens for phages GIL01 and GIL16, and, subsequently, the following life traits were compared among the strains: kinetics of growth, metabolic profiles, antibiotics susceptibility, biofilm formation, swarming motility, and sporulation. The results revealed that GIL01 and GIL16 lysogeny has a significant influence on the bacterial growth, sporulation rate, biofilm formation, and swarming motility of B. thuringiensis. No changes in metabolic profiles or antibiotic susceptibilities were detected. These findings provide evidence that tectiviruses have a putative role in the B. thuringiensis life cycle as adapters of life traits with ecological advantages.
[Mh] Termos MeSH primário: Bacillus thuringiensis/fisiologia
Bacteriófagos/fisiologia
Biofilmes
Lisogenia
Tectiviridae/fisiologia
[Mh] Termos MeSH secundário: Bacillus thuringiensis/genética
Bacillus thuringiensis/crescimento & desenvolvimento
Bacillus thuringiensis/virologia
Esporos Bacterianos/genética
Esporos Bacterianos/crescimento & desenvolvimento
Esporos Bacterianos/fisiologia
Esporos Bacterianos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1507
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140928
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01869-14


  3 / 29 MEDLINE  
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[PMID]:24795369
[Au] Autor:Gillis A; Mahillon J
[Ad] Endereço:Laboratory of Food and Environmental Microbiology, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
[Ti] Título:Prevalence, genetic diversity, and host range of tectiviruses among members of the Bacillus cereus group.
[So] Source:Appl Environ Microbiol;80(14):4138-52, 2014 Jul.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:GIL01, Bam35, GIL16, AP50, and Wip1 are tectiviruses preying on the Bacillus cereus group. Despite the significant contributions of phages in different biological processes, little is known about the dealings taking place between tectiviruses and their Gram-positive bacterial hosts. Therefore, this work focuses on characterizing the interactions between tectiviruses and the B. cereus group by assessing their occurrence and genetic diversity and evaluating their host range. To study the occurrence of tectiviruses in the B. cereus group, 2,000 isolates were evaluated using primers designed to be specific to two variable regions detected in previously described elements. PCR and propagation tests revealed that tectivirus-like elements occurred in less than 3% of the isolates. Regardless of this limited distribution, several novel tectiviruses were found, and partial DNA sequencing indicated that a greater diversity exists within the family Tectiviridae. Analyses of the selected variable regions, along with their host range, showed that tectiviruses in the B. cereus group can be clustered mainly into two different groups: the ones infecting B. anthracis and those isolated from other B. cereus group members. In order to address the host range of some novel tectiviruses, 120 strains were tested for sensitivity. The results showed that all the tested tectiviruses produced lysis in at least one B. cereus sensu lato strain. Moreover, no simple relationship between the infection patterns of the tectiviruses and their diversity was found.
[Mh] Termos MeSH primário: Bacillus cereus/virologia
Especificidade de Hospedeiro/genética
Tectiviridae/classificação
[Mh] Termos MeSH secundário: Bacillus cereus/classificação
Meios de Cultura/química
Primers do DNA
DNA Bacteriano/genética
DNA Viral/genética
Genes Bacterianos
Genes Virais
Variação Genética
Alinhamento de Sequência
Análise de Sequência de DNA
Tectiviridae/genética
Tectiviridae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media); 0 (DNA Primers); 0 (DNA, Bacterial); 0 (DNA, Viral)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140506
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.00912-14


  4 / 29 MEDLINE  
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[PMID]:23893110
[Au] Autor:Kan S; Fornelos N; Schuch R; Fischetti VA
[Ad] Endereço:Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, New York, USA.
[Ti] Título:Identification of a ligand on the Wip1 bacteriophage highly specific for a receptor on Bacillus anthracis.
[So] Source:J Bacteriol;195(19):4355-64, 2013 Oct.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tectiviridae is a family of tailless bacteriophages with Gram-negative and Gram-positive hosts. The family model PRD1 and its close relatives all infect a broad range of enterobacteria by recognizing a plasmid-encoded conjugal transfer complex as a receptor. In contrast, tectiviruses with Gram-positive hosts are highly specific to only a few hosts within the same bacterial species. The cellular determinants that account for the observed specificity remain unknown. Here we present the genome sequence of Wip1, a tectivirus that infects the pathogen Bacillus anthracis. The Wip1 genome is related to other tectiviruses with Gram-positive hosts, notably, AP50, but displays some interesting differences in its genome organization. We identified Wip1 candidate genes for the viral spike complex, the structure located at the capsid vertices and involved in host receptor binding. Phage adsorption and inhibition tests were combined with immunofluorescence microscopy to show that the Wip1 gene product p23 is a receptor binding protein. His-p23 also formed a stable complex with p24, a Wip1 protein of unknown function, suggesting that the latter is involved with p23 in host cell recognition. The narrow host range of phage Wip1 and the identification of p23 as a receptor binding protein offer a new range of suitable tools for the rapid identification of B. anthracis.
[Mh] Termos MeSH primário: Bacillus anthracis/metabolismo
Receptores Virais/fisiologia
Tectiviridae/fisiologia
[Mh] Termos MeSH secundário: Bacillus anthracis/citologia
Clonagem Molecular
DNA Viral/genética
Regulação Bacteriana da Expressão Gênica
Regulação Viral da Expressão Gênica
Genoma Viral
Ligantes
Microscopia de Fluorescência
Dados de Sequência Molecular
Receptores Virais/genética
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Ligands); 0 (Receptors, Virus)
[Em] Mês de entrada:1311
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130730
[St] Status:MEDLINE
[do] DOI:10.1128/JB.00655-13


  5 / 29 MEDLINE  
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[PMID]:23103336
[Au] Autor:Jalasvuori M; Palmu S; Gillis A; Kokko H; Mahillon J; Bamford JK; Fornelos N
[Ad] Endereço:Center of Excellence in Biological Interactions, Department of Biological and Environmental Science and Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014 Jyväskylä, Finland.
[Ti] Título:Identification of five novel tectiviruses in Bacillus strains: analysis of a highly variable region generating genetic diversity.
[So] Source:Res Microbiol;164(2):118-26, 2013 Feb-Mar.
[Is] ISSN:1769-7123
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Our biosphere is abundant with unique and small genes for which no homologs are known. These genes, often referred to as orphans or ORFans, are commonly found in bacteriophage genomes but their origins remain unclear. We discovered five novel tectivirus-like genetic elements by screening more than five-hundred Bacillus strains. A highly variable region (HVR) of these viruses was shown to harbor ORFans in most of these otherwise well-conserved bacteriophages. Previous studies demonstrated that mutations close to this region dramatically alter bacteriophage gene regulation, suggesting that the acquisition of those ORFans may provide a source of genetic diversity that is then subject to genetic selection during bacteriophage evolution.
[Mh] Termos MeSH primário: Fagos Bacilares/classificação
Fagos Bacilares/isolamento & purificação
Bacillus/virologia
Variação Genética
Tectiviridae/classificação
Tectiviridae/isolamento & purificação
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
DNA Viral/química
DNA Viral/genética
Microscopia Eletrônica de Transmissão
Dados de Sequência Molecular
Alinhamento de Sequência
Análise de Sequência de DNA
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:130219
[Lr] Data última revisão:
130219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121030
[St] Status:MEDLINE


  6 / 29 MEDLINE  
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[PMID]:18791014
[Au] Autor:Sozhamannan S; McKinstry M; Lentz SM; Jalasvuori M; McAfee F; Smith A; Dabbs J; Ackermann HW; Bamford JK; Mateczun A; Read TD
[Ad] Endereço:Naval Medical Research Center, Silver Spring, Maryland 20910, USA. Shanmuga.Sozhamannan@med.navy.mil
[Ti] Título:Molecular characterization of a variant of Bacillus anthracis-specific phage AP50 with improved bacteriolytic activity.
[So] Source:Appl Environ Microbiol;74(21):6792-6, 2008 Nov.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genome sequence of a Bacillus anthracis-specific clear plaque mutant phage, AP50c, contains 31 open reading frames spanning 14,398 bp, has two mutations compared to wild-type AP50t, and has a colinear genome architecture highly similar to that of gram-positive Tectiviridae phages. Spontaneous AP50c-resistant B. anthracis mutants exhibit a mucoid colony phenotype.
[Mh] Termos MeSH primário: Fagos Bacilares/genética
Bacillus anthracis/virologia
DNA Viral/genética
Genoma Viral
[Mh] Termos MeSH secundário: Bacteriólise
Sequência de Bases
Ordem dos Genes
Dados de Sequência Molecular
Mutação de Sentido Incorreto
Mutação Puntual
Análise de Sequência de DNA
Sintenia
Tectiviridae/genética
Ensaio de Placa Viral
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:0812
[Cu] Atualização por classe:140903
[Lr] Data última revisão:
140903
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080916
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01124-08


  7 / 29 MEDLINE  
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[PMID]:18657283
[Au] Autor:Jaatinen ST; Happonen LJ; Laurinmäki P; Butcher SJ; Bamford DH
[Ad] Endereço:Department of Biological and Environmental Sciences and Institute of Biotechnology, Biocenter 2, FIN-00014, University of Helsinki, Finland.
[Ti] Título:Biochemical and structural characterisation of membrane-containing icosahedral dsDNA bacteriophages infecting thermophilic Thermus thermophilus.
[So] Source:Virology;379(1):10-9, 2008 Sep 15.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Icosahedral dsDNA viruses isolated from hot springs and proposed to belong to the Tectiviridae family infect the gram-negative thermophilic Thermus thermophilus bacterium. Seven such viruses were obtained from the Promega Corporation collection. The structural protein patterns of three of these viruses, growing to a high titer, appeared very similar but not identical. The most stable virus, P23-77, was chosen for more detailed studies. Analysis of highly purified P23-77 by thin layer chromatography for neutral lipids showed lipid association with the virion. Cryo-EM based three-dimensional image reconstruction of P23-77 to 1.4 nm resolution revealed an icosahedrally-ordered protein coat, with spikes on the vertices, and an internal membrane. The capsid architecture of P23-77 is most similar to that of the archaeal virus SH1. These findings further complicate the grouping of icosahedrally-symmetric viruses containing an inner membrane. We propose a single superfamily or order with members in several viral families.
[Mh] Termos MeSH primário: Bacteriófagos/química
Bacteriófagos/ultraestrutura
Tectiviridae/química
Tectiviridae/ultraestrutura
Thermus thermophilus/virologia
[Mh] Termos MeSH secundário: Bacteriófagos/classificação
Bacteriófagos/isolamento & purificação
Microscopia Crioeletrônica
Fontes Termais/virologia
Lipídeos/análise
Microscopia Eletrônica de Transmissão
Modelos Moleculares
Estrutura Quaternária de Proteína
Estrutura Terciária de Proteína
Tectiviridae/classificação
Tectiviridae/isolamento & purificação
Ensaio de Placa Viral
Proteínas Estruturais Virais/isolamento & purificação
Vírion/química
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lipids); 0 (Viral Structural Proteins)
[Em] Mês de entrada:0809
[Cu] Atualização por classe:120430
[Lr] Data última revisão:
120430
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080729
[St] Status:MEDLINE
[do] DOI:10.1016/j.virol.2008.06.023


  8 / 29 MEDLINE  
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[PMID]:18363238
[Au] Autor:Ackermann HW
[Ad] Endereço:Department of Medical Biology, Laval University, Quebec, Canada.
[Ti] Título:Salmonella phages examined in the electron microscope.
[So] Source:Methods Mol Biol;394:213-34, 2007.
[Is] ISSN:1064-3745
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Out of 177 surveyed bacteriophages, 161 (91%) are tailed and belong to the Myoviridae, Siphoviridae, and Podoviridae families (43, 55, and 59 viruses, respectively). Sixteen filamentous or isometric phages are members of the Inoviridae, Leviviridae, Microviridae, and Tectiviridae families (9%). Many tailed phages belong to established phage genera (P22, T1, T5, and T7), which are widespread in enterobacteria and other Gram-negatives of the Proteobacteria phylum.
[Mh] Termos MeSH primário: Fagos de Salmonella/ultraestrutura
Salmonella/virologia
[Mh] Termos MeSH secundário: Bacteriófago P22/ultraestrutura
Tipagem de Bacteriófagos
Inoviridae/classificação
Inoviridae/ultraestrutura
Leviviridae/classificação
Leviviridae/ultraestrutura
Microscopia Eletrônica de Transmissão
Microviridae/classificação
Microviridae/ultraestrutura
Myoviridae/classificação
Myoviridae/ultraestrutura
Podoviridae/classificação
Podoviridae/ultraestrutura
Fagos de Salmonella/classificação
Siphoviridae/classificação
Siphoviridae/ultraestrutura
Tectiviridae/classificação
Tectiviridae/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:0804
[Cu] Atualização por classe:080326
[Lr] Data última revisão:
080326
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080328
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-59745-512-1_11


  9 / 29 MEDLINE  
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[PMID]:16338410
[Au] Autor:Laurinmäki PA; Huiskonen JT; Bamford DH; Butcher SJ
[Ad] Endereço:Institute of Biotechnology and Department of Biological and Environmental Sciences, Viikki Biocenter, University of Helsinki, P.O. Box 65 (Viikinkaari 1), 00014 University of Helsinki, Finland.
[Ti] Título:Membrane proteins modulate the bilayer curvature in the bacterial virus Bam35.
[So] Source:Structure;13(12):1819-28, 2005 Dec.
[Is] ISSN:0969-2126
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Biological membranes control the flow of molecules into and out of cells, and they transmit information about the milieu. Structural studies of membrane-containing viruses provide one way to study these membranes in situ. Cryo-electron microscopy and image reconstruction of bacteriophage Bam35 to 7.3 A resolution revealed a membrane bilayer constrained within an icosahedrally symmetric pseudo T = 25 capsid. A total of 60 large transmembrane protein complexes affect the curvature and thickness of the membrane. Here, we describe these membrane parameters quantitatively. Furthermore, we show that Bam35 differs from bacteriophage PRD1 in these parameters, even though the two viruses share the same principles of capsid architecture. Most notably, each virus possesses a tape measure protein suggesting a general mechanism for capsid size determination in icosahedral viruses.
[Mh] Termos MeSH primário: Bacillus thuringiensis/virologia
Capsídeo/ultraestrutura
Proteínas de Membrana/ultraestrutura
Tectiviridae/ultraestrutura
Proteínas Virais/ultraestrutura
[Mh] Termos MeSH secundário: Bacteriófago PRD1/fisiologia
Bacteriófago PRD1/ultraestrutura
Microscopia Crioeletrônica
Bicamadas Lipídicas/química
Membranas/ultraestrutura
Tectiviridae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lipid Bilayers); 0 (Membrane Proteins); 0 (Viral Proteins)
[Em] Mês de entrada:0602
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:051213
[St] Status:MEDLINE


  10 / 29 MEDLINE  
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[PMID]:16338400
[Au] Autor:Fuller S
[Ti] Título:A PRD1 by another name?
[So] Source:Structure;13(12):1738-40, 2005 Dec.
[Is] ISSN:0969-2126
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Bacteriófago PRD1/ultraestrutura
Bicamadas Lipídicas/química
Tectiviridae/ultraestrutura
Proteínas Virais/ultraestrutura
[Pt] Tipo de publicação:COMMENT; NEWS
[Nm] Nome de substância:
0 (Lipid Bilayers); 0 (Viral Proteins)
[Em] Mês de entrada:0602
[Cu] Atualização por classe:051212
[Lr] Data última revisão:
051212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:051213
[St] Status:MEDLINE



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