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[PMID]: 28809152 [Au] Autor: Miller MM; Cornish TE; Creekmore TE; Fox K; Laegreid W; McKenna J; Vasquez M; Woods LW [Ad] Endereço: 1​University of Wyoming, Wyoming State Veterinary Laboratory, 1174 Snowy Range Road, Laramie, WY 82070, USA. [Ti] Título: Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus. [So] Source: J Gen Virol;98(9):2320-2328, 2017 Sep. [Is] ISSN: 1465-2099 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: We present the first complete genome sequence of Odocoileus hemionus deer adenovirus 1 (OdAdV-1). This virus can cause sporadic haemorrhagic disease in cervids, although epizootics with high mortality have occurred in California. OdAdV-1 has been placed in the genus Atadenovirus, based on partial hexon, pVIII and fibre genes. Ten field isolates recovered from naturally infected mule deer (Odocoileus hemionus), white-tailed deer (Odocoileus virginiana) and moose (Alces alces) from Wyoming, black-tailed deer (Odocoileus hemionus columbianus) from California, and Rocky Mountain elk (Cervus elaphus nelsoni) from Colorado and Washington state were sequenced. The genome lengths ranged from 30 620 to 30 699 bp, contained the predicted proteins and gene organization typical of members of genus Atadenovirus, and had a high percentage of A/T nucleotides (66.7 %). Phylogenic analysis found that the closest ancestry was with ruminant atadenoviruses, while a divergence of the hexon, polymerase and penton base proteins of more than 15 % supports classification as a new species. Genetic global comparison between the 10 isolates found an overall 99 % identity, but greater divergence was found between those recovered from moose and elk as compared to deer, and a single variable region contained most of these differences. Our findings demonstrate that OdAdV-1 is highly conserved between 10 isolates recovered from multiple related cervid species, but genotypic differences, largely localized to a variable region, define two strains. We propose that the virus type name be changed to cervid adenovirus 1, with the species name Cervid atadenovirus A. Sequence data were used to develop molecular assays for improved detection and genotyping. [Mh] Termos MeSH primário: Animais Selvagens/virologia Atadenovirus/isolamento & purificação Cervos/virologia Genoma Viral Ruminantes/virologia [Mh] Termos MeSH secundário: Animais Atadenovirus/classificação Atadenovirus/genética Sequência de Bases Sequência Conservada Genótipo Dados de Sequência Molecular Filogenia Análise de Sequência de DNA [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171115 [Lr] Data última revisão: 171115 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170816 [St] Status: MEDLINE [do] DOI: 10.1099/jgv.0.000880

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[PMID]: 28481913 [Au] Autor: Kang M; Cha SY; Jang HK [Ad] Endereço: Department of Veterinary Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Center for Poultry Diseases Control, Chonbuk National University, Iksan, South Korea. [Ti] Título: Tropism and infectivity of duck-derived egg drop syndrome virus in chickens. [So] Source: PLoS One;12(5):e0177236, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Egg drop syndrome virus (EDSV) can markedly decrease egg production in laying hens. Duck is the natural host of EDSV. EDSV derived from ducks abrogate egg drop in laying hens. We have previously confirmed that duck-derived EDSVs have a variety of replication activities in chick embryo liver (CEL) cells. However, it is currently unclear whether duck-derived EDSV could display tropism and adaptation in laying hens. This study assessed whether duck-derived EDSV can adapt to laying hens, and estimated the inducing factors. Complete genome sequences of duck-derived EDSVs (D11-JW-012, D11-JW-017, and D11-JW-032 isolates) with various replication efficiency in CEL cells and C10-GY-001 isolate causing disease in laying hens were analyzed to find their differences. Phylogenetic analysis of complete genome sequence revealed that C10-GY-001, D11-JW-032, and strain 127 virus as vaccine were clustered into the same group, with D11-JW-012 and D11-JW-017 clustered in another group. Comparison between D11-JW-012 isolate that poorly replicated and D11-JW-017 isolate that replicated well in CEL cells in same cluster revealed six amino acid differences on IVa2, DNA polymerase, endopeptidase, and DNA-binding protein. These amino acids might be key candidates enhancing cellular tropism in chicken. When the pathogenicities of these isolates in laying hens were compared, D11-JW-032 showed severe signs similar to 127 virus, D11-JW-017 showed intermediate signs, while D11-JW-012 showed almost no sign. Eleven amino acids differed between D11-JW-032 and D11-JW-017, and 17 amino acids were different between D11-JW-032 and D11-JW-012. These results suggest that EDSVs derived from ducks have various pathogenicities in laying hens. Key amino acid candidates might have altered their affinity to tropism of laying hens, causing difference pathogenicities. [Mh] Termos MeSH primário: Atadenovirus/patogenicidade Galinhas/virologia Tropismo Viral [Mh] Termos MeSH secundário: Animais Atadenovirus/classificação Atadenovirus/fisiologia Patos Filogenia Virulência [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170915 [Lr] Data última revisão: 170915 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170509 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0177236

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[PMID]: 27486139 [Au] Autor: Garcia-Morante B; Pénzes JJ; Costa T; Martorell J; Martínez J [Ad] Endereço: Servei de Diagnòstic de Patologia Veterinària, Departament de Sanitat i Anatomia Animals, Universitat Autònoma de Barcelona, Bellaterra, Spain (Garcia-Morante, Martínez)Hospital Clínic Veterinari, Universitat Autònoma de Barcelona, Bellaterra, Spain (Martorell)Centre de Recerca en Sanitat Animal (CR [Ti] Título: Hyperplastic stomatitis and esophagitis in a tortoise (Testudo graeca) associated with an adenovirus infection. [So] Source: J Vet Diagn Invest;28(5):579-83, 2016 Sep. [Is] ISSN: 1943-4936 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A 2-year-old female, spur-thighed tortoise (Testudo graeca) was presented with poor body condition (1/5) and weakness. Fecal analysis revealed large numbers of oxyurid-like eggs, and radiographs were compatible with gastrointestinal obstruction. Despite supportive medical treatment, the animal died. At gross examination, an intestinal obstruction was confirmed. Histopathology revealed severe hyperplastic esophagitis and stomatitis with marked epithelial cytomegaly and enormous basophilic intranuclear inclusion bodies. Electron microscopy examination revealed a large number of 60-80 nm, nonenveloped, icosahedral virions arranged in crystalline arrays within nuclear inclusions of esophageal epithelial cells, morphologically compatible with adenovirus-like particles. PCR for virus identification was performed with DNA extracted from formalin-fixed, paraffin-embedded tissues. A nested, consensus pan-adenovirus PCR and sequencing analysis showed a novel adenovirus. According to phylogenetic calculations, it clustered to genus Atadenovirus in contrast with all other chelonian adenoviruses described to date. The present report details the pathologic findings associated with an adenovirus infection restricted to the upper digestive tract. [Mh] Termos MeSH primário: Infecções por Adenoviridae/veterinária Atadenovirus/isolamento & purificação Tartarugas [Mh] Termos MeSH secundário: Infecções por Adenoviridae/complicações Infecções por Adenoviridae/diagnóstico Animais Atadenovirus/genética Diagnóstico Diferencial Esofagite/etiologia Esofagite/veterinária Feminino Filogenia Reação em Cadeia da Polimerase/veterinária Estomatite/etiologia Estomatite/veterinária [Pt] Tipo de publicação: CASE REPORTS; JOURNAL ARTICLE [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160804 [St] Status: MEDLINE [do] DOI: 10.1177/1040638716659903

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[PMID]: 26562056 [Au] Autor: Malenovská H [Ad] Endereço: Collection of Animal Pathogenic Microorganisms, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic. Electronic address: Malenovska@vri.cz. [Ti] Título: 3D rotating wall vessel and 2D cell culture of four veterinary virus pathogens: A comparison of virus yields, portions of infectious particles and virus growth curves. [So] Source: J Virol Methods;228:10-5, 2016 Feb. [Is] ISSN: 1879-0984 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Only very few comparative studies have been performed that evaluate general trends of virus growth under 3D in comparison with 2D cell culture conditions. The aim of this study was to investigate differences when four animal viruses are cultured in 2D and 3D. Suid herpesvirus 1 (SuHV-1), Vesicular stomatitis virus (VSIV), Bovine adenovirus (BAdV) and Bovine parainfluenza 3 virus (BPIV-3) were cultivated in 3D rotating wall vessels (RWVs) and conventional 2D cultures. The production of virus particles, the portion of infectious particles, and the infectious growth curves were compared. For all viruses, the production of virus particles (related to cell density), including the non-infectious ones, was lower in 3D than in 2D culture. The production of only infectious particles was significantly lower in BAdV and BPIV-3 in 3D cultures in relation to cell density. The two cultivation approaches resulted in significantly different virus particle-to-TCID50 ratios in three of the four viruses: lower in SuHV-1 and BPIV-3 and higher in BAdV in 3D culture. The infectious virus growth rates were not significantly different in all viruses. Although 3D RWV culture resulted in lower production of virus particles compared to 2D systems, the portion of infectious particles was higher for some viruses. [Mh] Termos MeSH primário: Atadenovirus/crescimento & desenvolvimento Técnicas de Cultura de Células Herpesvirus Suídeo 1/crescimento & desenvolvimento Vírus da Parainfluenza 3 Bovina/crescimento & desenvolvimento Vírus da Estomatite Vesicular Indiana/crescimento & desenvolvimento Cultura de Vírus/métodos [Mh] Termos MeSH secundário: Animais Atadenovirus/fisiologia Atadenovirus/ultraestrutura Bovinos Técnicas de Cultura de Células/instrumentação Técnicas de Cultura de Células/métodos Cercopithecus aethiops Cães Herpesvirus Suídeo 1/fisiologia Herpesvirus Suídeo 1/ultraestrutura Células Madin Darby de Rim Canino Vírus da Parainfluenza 3 Bovina/fisiologia Vírus da Parainfluenza 3 Bovina/ultraestrutura Suínos Células Vero Vírus da Estomatite Vesicular Indiana/fisiologia Vírus da Estomatite Vesicular Indiana/ultraestrutura Replicação Viral [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151113 [St] Status: MEDLINE

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[PMID]: 26200954 [Au] Autor: Huang J; Tan D; Wang Y; Liu C; Xu J; Wang J [Ad] Endereço: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: hjj7920@126.com. [Ti] Título: Egg drop syndrome virus enters duck embryonic fibroblast cells via clathrin-mediated endocytosis. [So] Source: Virus Res;210:69-76, 2015 Dec 02. [Is] ISSN: 1872-7492 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Previous studies of egg drop syndrome virus (EDSV) is restricted to serological surveys, disease diagnostics, and complete viral genome analysis. Consequently, the infection characteristics and entry routes of EDSV are poorly understood. Therefore, we aimed to explore the entry pathway of EDSV into duck embryonic fibroblast (DEF) cells as well as the infection characteristics and proliferation of EDSV in primary DEF and primary chicken embryo liver (CEL) cells. Transmission electron microscopy revealed that the virus triggered DEF cell membrane invagination as early as 10 min post-infection and that integrated endocytic vesicles formed at 20 min post-infection. The virus yield in EDSV-infected DEF cells treated with chlorpromazine (CPZ), sucrose, methyl-ß-cyclodextrin (MßCD), or NH4Cl was measured by quantitative real-time PCR. Compared with the mock treatment, CPZ and sucrose greatly inhibited the production of viral progeny in a dose-dependent manner, while MßCD treatment did not result in a significant difference. Furthermore, NH4Cl had a strong inhibitory effect on the production of EDSV progeny. In addition, indirect immunofluorescence demonstrated that virus particles clustered on the surface of DEF cells treated with CPZ or sucrose. These results indicate that EDSV enters DEF cells through clathrin-mediated endocytosis followed by a pH-dependent step, which is similar to the mechanism of entry of human adenovirus types 2 and 5. [Mh] Termos MeSH primário: Atadenovirus/fisiologia Clatrina/metabolismo Endocitose Fibroblastos/virologia Interações Hospedeiro-Patógeno Internalização do Vírus [Mh] Termos MeSH secundário: Animais Atadenovirus/ultraestrutura Membrana Celular/ultraestrutura Membrana Celular/virologia Células Cultivadas Galinhas Patos Feminino Técnica Indireta de Fluorescência para Anticorpo Hepatócitos/virologia Microscopia Eletrônica de Transmissão Fatores de Tempo Replicação Viral [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Clathrin) [Em] Mês de entrada: 1608 [Cu] Atualização por classe: 151110 [Lr] Data última revisão: 151110 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150723 [St] Status: MEDLINE

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[PMID]: 25994880 [Au] Autor: Nguyen TH; Vidovszky MZ; Ballmann MZ; Sanz-Gaitero M; Singh AK; Harrach B; Benko M; van Raaij MJ [Ad] Endereço: Departamento de Estructura de Macromoleculas, Centro Nacional de Biotecnologia (CNB-CSIC), calle Darwin 3, 28049, Madrid, Spain. th.nguyen@cnb.csic.es. [Ti] Título: Crystal structure of the fibre head domain of bovine adenovirus 4, a ruminant atadenovirus. [So] Source: Virol J;12:81, 2015 May 22. [Is] ISSN: 1743-422X [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: In adenoviruses, primary host cell recognition is generally performed by the head domains of their homo-trimeric fibre proteins. This first interaction is reversible. A secondary, irreversible interaction subsequently takes place via other adenovirus capsid proteins and leads to a productive infection. Although many fibre head structures are known for human mastadenoviruses, not many animal adenovirus fibre head structures have been determined, especially not from those belonging to adenovirus genera other than Mastadenovirus. METHODS: We constructed an expression vector for the fibre head domain from a ruminant atadenovirus, bovine adenovirus 4 (BAdV-4), consisting of amino acids 414-535, expressed the protein in Escherichia coli, purified it by metal affinity and cation exchange chromatography and crystallized it. The structure was solved using single isomorphous replacement plus anomalous dispersion of a mercury derivative and refined against native data that extended to 1.2 Å resolution. RESULTS: Like in other adenoviruses, the BAdV-4 fibre head monomer contains a beta-sandwich consisting of ABCJ and GHID sheets. The topology is identical to the fibre head of the other studied atadenovirus, snake adenovirus 1 (SnAdV-1), including the alpha-helix in the DG-loop, despite of them having a sequence identity of only 15 %. There are also differences which may have implications for ligand binding. Beta-strands G and H are longer and differences in several surface-loops and surface charge are observed. CONCLUSIONS: Chimeric adenovirus fibres have been used to retarget adenovirus-based anti-cancer and gene therapy vectors. Ovine adenovirus 7 (OAdV-7), another ruminant atadenovirus, is intensively tested as a basis for such a vector. Here, we present the high-resolution atomic structure of the BAdV-4 fibre head domain, the second atadenovirus fibre head structure known and the first of an atadenovirus that infects a mammalian host. Future research should focus on the receptor-binding properties of these fibre head domains. [Mh] Termos MeSH primário: Atadenovirus/química Proteínas do Capsídeo/química [Mh] Termos MeSH secundário: Animais Bovinos Cristalografia por Raios X Modelos Moleculares Conformação Proteica [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Capsid Proteins); 0 (hexon capsid protein, Adenovirus) [Em] Mês de entrada: 1601 [Cu] Atualização por classe: 170220 [Lr] Data última revisão: 170220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150522 [St] Status: MEDLINE [do] DOI: 10.1186/s12985-015-0309-1

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[PMID]: 26035955 [Au] Autor: Mohapatra N; Kataria JM; Chakraborty S; Dhama K [Ti] Título: Egg Drop Syndrome-76 (EDS-76) in Japanese quails (Coturnix coturnix japonica): an experimental study revealing pathology, effect on egg production/quality and immune responses. [So] Source: Pak J Biol Sci;17(6):821-8, 2014 Jun. [Is] ISSN: 1028-8880 [Cp] País de publicação: Pakistan [La] Idioma: eng [Ab] Resumo: Egg Drop Syndrome-76 (EDS-76) is a recognized disease of chickens and Japanese Quails, which is of high economic importance due to its drastic negative effects on egg production in laying birds. The aim of the present study was to better understand the EDS-76 viral disease process in Japanese quails (Coturnix coturnix japonica), since very limited studies have been conducted in this species of birds. For this purpose, an experimental study was conducted with infection of EDS-76 virus in laying Japanese quails to reveal pathology, effect on egg production/quality and immune responses of this virus in these birds. By 7, 9 and 13-15 Days Post Infection (DPI), drop as well as aberrant egg production and lower mean egg quality were observed compared to control birds. Significant histopathological changes were observed in genitalia and spleen. Haemagglutination Inhibition (HI) and Enzyme Linked Immunosorbent Assay (ELISA) titres rose rapidly by 2nd week when it became maximum; thereafter declined and maintained at low levels up to 10 week post infection. The mean total protein values in infected quail gradually increased to 4.10±0.05/100 mL without any change in mean albumen value at 12 DPI. In conclusion, the course of the EDS-76 is significant not only in chickens but also in quails even though it occurs occasionally in quails. Explorative pathological, blood biochemical and immunological studies are suggested during EDS-76 viral disease course in quails. This would aid in formulating effective disease prevention and control measures for this economically important disease of poultry. [Mh] Termos MeSH primário: Infecções por Adenoviridae/patologia Atadenovirus/patogenicidade [Mh] Termos MeSH secundário: Infecções por Adenoviridae/imunologia Infecções por Adenoviridae/fisiopatologia Animais Coturnix [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1506 [Cu] Atualização por classe: 150603 [Lr] Data última revisão: 150603 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 150604 [St] Status: MEDLINE

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[PMID]: 25056898 [Au] Autor: Pénzes JJ; Menéndez-Conejero R; Condezo GN; Ball I; Papp T; Doszpoly A; Paradela A; Pérez-Berná AJ; López-Sanz M; Nguyen TH; van Raaij MJ; Marschang RE; Harrach B; Benko M; San Martín C [Ad] Endereço: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary. [Ti] Título: Molecular characterization of a lizard adenovirus reveals the first atadenovirus with two fiber genes and the first adenovirus with either one short or three long fibers per penton. [So] Source: J Virol;88(19):11304-14, 2014 Oct. [Is] ISSN: 1098-5514 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: UNLABELLED: Although adenoviruses (AdVs) have been found in a wide variety of reptiles, including numerous squamate species, turtles, and crocodiles, the number of reptilian adenovirus isolates is still scarce. The only fully sequenced reptilian adenovirus, snake adenovirus 1 (SnAdV-1), belongs to the Atadenovirus genus. Recently, two new atadenoviruses were isolated from a captive Gila monster (Heloderma suspectum) and Mexican beaded lizards (Heloderma horridum). Here we report the full genomic and proteomic characterization of the latter, designated lizard adenovirus 2 (LAdV-2). The double-stranded DNA (dsDNA) genome of LAdV-2 is 32,965 bp long, with an average G+C content of 44.16%. The overall arrangement and gene content of the LAdV-2 genome were largely concordant with those in other atadenoviruses, except for four novel open reading frames (ORFs) at the right end of the genome. Phylogeny reconstructions and plesiomorphic traits shared with SnAdV-1 further supported the assignment of LAdV-2 to the Atadenovirus genus. Surprisingly, two fiber genes were found for the first time in an atadenovirus. After optimizing the production of LAdV-2 in cell culture, we determined the protein compositions of the virions. The two fiber genes produce two fiber proteins of different sizes that are incorporated into the viral particles. Interestingly, the two different fiber proteins assemble as either one short or three long fiber projections per vertex. Stoichiometry estimations indicate that the long fiber triplet is present at only one or two vertices per virion. Neither triple fibers nor a mixed number of fibers per vertex had previously been reported for adenoviruses or any other virus. IMPORTANCE: Here we show that a lizard adenovirus, LAdV-2, has a penton architecture never observed before. LAdV-2 expresses two fiber proteins-one short and one long. In the virion, most vertices have one short fiber, but a few of them have three long fibers attached to the same penton base. This observation raises new intriguing questions on virus structure. How can the triple fiber attach to a pentameric vertex? What determines the number and location of each vertex type in the icosahedral particle? Since fibers are responsible for primary attachment to the host, this novel architecture also suggests a novel mode of cell entry for LAdV-2. Adenoviruses have a recognized potential in nanobiomedicine, but only a few of the more than 200 types found so far in nature have been characterized in detail. Exploring the taxonomic wealth of adenoviruses should improve our chances to successfully use them as therapeutic tools. [Mh] Termos MeSH primário: Atadenovirus/genética Proteínas do Capsídeo/genética DNA Viral/genética Genoma Viral Lagartos/virologia Vírion/genética [Mh] Termos MeSH secundário: Sequência de Aminoácidos Animais Atadenovirus/classificação Atadenovirus/ultraestrutura Composição de Bases Sequência de Bases Proteínas do Capsídeo/ultraestrutura DNA/genética Expressão Gênica Dados de Sequência Molecular Fases de Leitura Aberta Filogenia Vírion/ultraestrutura [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Capsid Proteins); 0 (DNA, Viral); 0 (hexon capsid protein, Adenovirus); 9007-49-2 (DNA) [Em] Mês de entrada: 1411 [Cu] Atualização por classe: 170220 [Lr] Data última revisão: 170220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 140725 [St] Status: MEDLINE [do] DOI: 10.1128/JVI.00306-14

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[PMID]: 24590667 [Au] Autor: Schybli M; Sigrist B; Hess M; van Leerdam B; Hoop RK; Vögtlin A [Ad] Endereço: 1Andrea Vögtlin, Institute of Virology and Immunology (IVI), Sensemattstraße 293, 3147 Mittelhäusern, Switzerland. andrea.voegtlin@ivi.admin.ch. [Ti] Título: Development of a new real-time polymerase chain reaction assay to detect Duck adenovirus A DNA and application to samples from Swiss poultry flocks. [So] Source: J Vet Diagn Invest;26(2):189-94, 2014 Mar. [Is] ISSN: 1943-4936 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Between 2008 and 2012, commercial Swiss layer and layer breeder flocks experiencing problems in laying performance were sampled and tested for infection with Duck adenovirus A (DAdV-A; previously known as Egg drop syndrome 1976 virus). Organ samples from birds sent for necropsy as well as blood samples from living animals originating from the same flocks were analyzed. To detect virus-specific DNA, a newly developed quantitative real-time polymerase chain reaction method was applied, and the presence of antibodies against DAdV-A was tested using a commercially available enzyme-linked immunosorbent assay. In 5 out of 7 investigated flocks, viral DNA was detected in tissues. In addition, antibodies against DAdV-A were detected in all of the flocks. [Mh] Termos MeSH primário: Infecções por Adenoviridae/veterinária Atadenovirus/isolamento & purificação Galinhas Doenças das Aves Domésticas/virologia Reação em Cadeia da Polimerase em Tempo Real/veterinária [Mh] Termos MeSH secundário: Infecções por Adenoviridae/diagnóstico Infecções por Adenoviridae/epidemiologia Animais Anticorpos Antivirais/sangue Atadenovirus/genética DNA Viral/isolamento & purificação Ensaio de Imunoadsorção Enzimática/veterinária Feminino Doenças das Aves Domésticas/diagnóstico Doenças das Aves Domésticas/epidemiologia Sensibilidade e Especificidade Organismos Livres de Patógenos Específicos Suíça/epidemiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antibodies, Viral); 0 (DNA, Viral) [Em] Mês de entrada: 1411 [Cu] Atualização por classe: 140327 [Lr] Data última revisão: 140327 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 140305 [St] Status: MEDLINE [do] DOI: 10.1177/1040638714523426

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[PMID]: 24569225 [Au] Autor: Ball I; Hoferer M; Marschang RE [Ad] Endereço: 1Inna Ball, Fachgebiet für Umwelt und Tierhygiene, University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, Germany. inna-ball@gmx.de. [Ti] Título: Establishment of an agamid cell line and isolation of adenoviruses from central bearded dragons (Pogona vitticeps). [So] Source: J Vet Diagn Invest;26(2):221-5, 2014 Mar. [Is] ISSN: 1943-4936 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A cell line was established from whole 6-8-week-old central bearded dragon (Pogona vitticeps) embryos. Cells were mid-sized and showed an elongated and polymorphic form. The cell line grew in a monolayer and has been serially passaged for 17 passages at time of publication. This cell line has been used with samples from adenovirus polymerase chain reaction (PCR)-positive bearded dragons, and 2 virus isolates have been obtained so far. The isolates show a clear cytopathic effect in inoculated cells. Both virus isolates have been serially passaged on this cell line, and have been identified by PCR amplification and sequencing of a portion of the DNA-dependent DNA polymerase gene and show 100% nucleotide identity to the corresponding region of an agamid adenovirus. Electron microscopic examination of supernatant from infected cells demonstrated the presence of nonenveloped particles, with a diameter of approximately 80 nm in both virus isolates. [Mh] Termos MeSH primário: Atadenovirus/isolamento & purificação Lagartos/virologia Cultura de Vírus/métodos [Mh] Termos MeSH secundário: Animais Atadenovirus/fisiologia Linhagem Celular Efeito Citopatogênico Viral/fisiologia Lagartos/embriologia Inoculações Seriadas Especificidade da Espécie [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1411 [Cu] Atualização por classe: 140327 [Lr] Data última revisão: 140327 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 140227 [St] Status: MEDLINE [do] DOI: 10.1177/1040638714523615

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		Pesquisar	no campo
1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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