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[PMID]:28671077
[Au] Autor:Balboni A; Dondi F; Agnoli C; Verin R; Gruarin M; Morini M; Battilani M
[Ad] Endereço:Department of Veterinary Medical Sciences, Alma Mater Studiorum-University of Bologna, Ozzano Emilia, Bologna, Italy.
[Ti] Título:Novel sequence variants of viral hexon and fibre genes in two dogs with canine adenovirus type 1-associated disease.
[So] Source:Vet J;223:73-75, 2017 May.
[Is] ISSN:1532-2971
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:There is little information on sequence variation of canine adenovirus type 1 (CAdV-1), the aetiological agent of infectious canine hepatitis (ICH). This study reports hexon and fibre gene sequence variants of CAdV-1 in a dog with systemic ICH and a dog with the ocular form of the disease ('blue eye') in Northern Italy in 2013. One of the sequence variants matched a CAdV-1 fox sequence previously detected in Italy.
[Mh] Termos MeSH primário: Infecções por Adenoviridae/veterinária
Adenovirus Caninos/genética
Proteínas do Capsídeo/genética
Oftalmopatias/veterinária
Variação Genética/genética
Hepatite Infecciosa Canina/virologia
[Mh] Termos MeSH secundário: Infecções por Adenoviridae/virologia
Sequência de Aminoácidos
Animais
Sequência de Bases
Proteínas do Capsídeo/química
Cães
Oftalmopatias/virologia
Itália
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (hexon capsid protein, Adenovirus)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE


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[PMID]:27742446
[Au] Autor:Li XZ; Lv L; Zhang X; Anchang KY; Abdullahi AY; Tu L; Wang X; Xia L; Zhang XX; Feng W; Lu C; Li S; Yuan ZG
[Ad] Endereço:Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, 483 Wushan Rd, Tianhe District, Guangzhou 510642, PR China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, PR China.
[Ti] Título:Recombinant canine adenovirus type-2 expressing TgROP16 provides partial protection against acute Toxoplasma gondii infection in mice.
[So] Source:Infect Genet Evol;45:447-453, 2016 Nov.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We previously demonstrated that the survival time of BALB/c mice challenged with Toxoplasma gondii RH strain was prolonged by immunising the mice with a eukaryotic vector expressing the protein ROP16 of T. gondii. Building upon previous findings, we are exploring improved vaccination strategies to enhance protection. In this work, a novel recombinant canine adenovirus type 2 expressing ROP16 (CAV-2-ROP16) of T. gondii was constructed and identified to express ROP16 in Madin-Darby canine kidney cells (MDCK) cells by western blot (WB) and indirect immunofluorescence (IFA) assays. Intramuscular immunisation of BALB/c mice with CAV-2-ROP16 was performed to evaluate the humoral and cellular immune responses. This vaccination triggered significant humoral and cellular responses, including ROP16-stimulated lymphoproliferation (P<0.05). Compared to control groups, the CAV-2-ROP16 immunised mice had high production of IFN-γ, IL-2 and IL-12 (P<0.05), with a predominance of IgG2a production, but not IL-10 (P>0.05), revealing that a predominant Th1-type response had developed. The cell-mediated cytotoxic activity with high levels of IFN-γ and TNF-α was significantly increased in both CD4 and CD8 T-cell compartments in the mice immunised with CAV-2-ROP16 (P<0.05), compared to three control groups. In addition, when immunised mice were challenged with the RH strain of T. gondii, they showed a significantly increased survival rate (25%) 80days post infection compared with control mice that all died within seven days (P<0.05). The 25% protection rate elicited by the recombinant virus CAV-2-ROP16 has not been achieved in the field of anti-T. gondii vaccination until now. Our work presents the successful use of recombinant virus CAV-2-ROP16 in vaccination protocols to protect against intraperitoneal challenge with the virulent RH strain of T. gondii. This system was shown to be extremely efficient in eliciting humoral and cellular immune responses that led to a significant improvement in survival time in mice.
[Mh] Termos MeSH primário: Adenovirus Caninos/genética
Proteínas Tirosina Quinases/imunologia
Proteínas de Protozoários/imunologia
Vacinas Protozoárias/imunologia
Toxoplasma/imunologia
Toxoplasmose Animal/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antiprotozoários/sangue
Citocinas/sangue
Cães
Feminino
Células Madin Darby de Rim Canino
Camundongos
Camundongos Endogâmicos BALB C
Proteínas Tirosina Quinases/química
Proteínas Tirosina Quinases/genética
Proteínas de Protozoários/química
Proteínas de Protozoários/genética
Vacinas Protozoárias/administração & dosagem
Vacinas Protozoárias/química
Vacinas Protozoárias/genética
Baço/citologia
Baço/imunologia
Toxoplasmose Animal/parasitologia
Vacinas de DNA/administração & dosagem
Vacinas de DNA/química
Vacinas de DNA/genética
Vacinas de DNA/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Cytokines); 0 (Protozoan Proteins); 0 (Protozoan Vaccines); 0 (Vaccines, DNA); EC 2.7.10.1 (Protein-Tyrosine Kinases); EC 2.7.10.1 (Rop16 protein, Toxoplasma gondii)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161016
[St] Status:MEDLINE


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[PMID]:27040113
[Au] Autor:Dowgier G; Mari V; Losurdo M; Larocca V; Colaianni ML; Cirone F; Lucente MS; Martella V; Buonavoglia C; Decaro N
[Ad] Endereço:Department of Veterinary Medicine, University of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy.
[Ti] Título:A duplex real-time PCR assay based on TaqMan technology for simultaneous detection and differentiation of canine adenovirus types 1 and 2.
[So] Source:J Virol Methods;234:1-6, 2016 Aug.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Canine adenoviruses are a major cause of disease in dogs, coyotes, red foxes and wolves, as well as in other carnivores and marine mammals. Canine adenovirus type 1 (CAdV-1) and canine adenovirus type 2 (CAdV-2) cause infectious canine hepatitis (ICH) and infectious tracheobronchitis (ITB), respectively. In this study, a duplex real-time PCR assay for simultaneous detection and characterisation of CAdV-1 and CAdV-2 was developed by using a single primer pair and virus-specific probes. The assay was validated testing standard DNAs produced on purpose and clinical samples of various matrices known to be positive for CAdV-1, CAdV-2 or both viruses. Precise calculation of DNA loads in samples containing a wide range of viral amounts was allowed by generating a standard curve for absolute quantification. The assay was proven to be highly specific, since no cross-reactions with the different CAdV type was observed, and sensitive, being able to detect less than 10 copies of CAdV-1/CAdV-2 DNA. The low intra-assay and interassay coefficient of variations demonstrated a high repeatability, thus confirming the potential use of this assay for quantitative detection of CAdV-1 and CAdV-2 for rapid diagnosis and epidemiological investigations.
[Mh] Termos MeSH primário: Adenovirus Caninos/isolamento & purificação
Hepatite Infecciosa Canina/diagnóstico
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Adenovirus Caninos/imunologia
Animais
Primers do DNA
Cães
Hepatite Infecciosa Canina/virologia
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160405
[St] Status:MEDLINE


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[PMID]:27033531
[Au] Autor:Pintore MD; Corbellini D; Chieppa MN; Vallino Costassa E; Florio CL; Varello K; Bozzetta E; Adriano D; Decaro N; Casalone C; Iulini B
[Ad] Endereço:Istituto Zoopro lattico Sperimentale del Piemonte, Liguria e Valle d'Aosta,Via Bologna 148, 10154 Torino, Italy.
[Ti] Título:Canine adenovirus type 1 and Pasteurella pneumotropica co­infection in a puppy.
[So] Source:Vet Ital;52(1):57-62, 2016 Jan-Mar.
[Is] ISSN:1828-1427
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:In 2008, a 2 months-old male German shepherd was presented with fever, depression, and evident organic wasting. The puppy died within 48 hours after the onset of clinical signs. A complete necropsy was performed. Bacteriological examination of samples from the brain, lung, liver, spleen, and bone marrow tested positive for Pasteurella pneumotropica. Histopathology demonstrated inflammatory and vascular lesions in the central nervous system and internal organs. Canine adenovirus type 1 nucleic acid was detected by polymerase chain reaction in the frozen brain but not in the formalin-fixed, paraffin-embedded liver and lung samples. The positive PCR was subsequently confirmed by indirect fluorescent antibody testing of the paraffin-embedded brain and liver sections. Although the liver is the primary site of viral damage, these laboratory findings suggest that Canine adenovirus type 1 infection should be included in the differential diagnosis of neuropathological diseases in dogs and that adenoviral infections could promote septicaemia caused by opportunistic pathogens.
[Mh] Termos MeSH primário: Adenovirus Caninos
Coinfecção
Doenças do Cão/microbiologia
Hepatite Infecciosa Canina/complicações
Infecções por Pasteurella/veterinária
Pasteurella pneumotropica
[Mh] Termos MeSH secundário: Animais
Cães
Masculino
Infecções por Pasteurella/complicações
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE
[do] DOI:10.12834/VetIt.270.934.1


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[PMID]:27004747
[Au] Autor:Carinhas N; Pais DA; Koshkin A; Fernandes P; Coroadinha AS; Carrondo MJ; Alves PM; Teixeira AP
[Ad] Endereço:IBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal.
[Ti] Título:Metabolic flux profiling of MDCK cells during growth and canine adenovirus vector production.
[So] Source:Sci Rep;6:23529, 2016 Mar 23.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Canine adenovirus vector type 2 (CAV2) represents an alternative to human adenovirus vectors for certain gene therapy applications, particularly neurodegenerative diseases. However, more efficient production processes, assisted by a greater understanding of the effect of infection on producer cells, are required. Combining [1,2-(13)C]glucose and [U-(13)C]glutamine, we apply for the first time (13)C-Metabolic flux analysis ((13)C-MFA) to study E1-transformed Madin-Darby Canine Kidney (MDCK) cells metabolism during growth and CAV2 production. MDCK cells displayed a marked glycolytic and ammoniagenic metabolism, and (13)C data revealed a large fraction of glutamine-derived labelling in TCA cycle intermediates, emphasizing the role of glutamine anaplerosis. (13)C-MFA demonstrated the importance of pyruvate cycling in balancing glycolytic and TCA cycle activities, as well as occurrence of reductive alphaketoglutarate (AKG) carboxylation. By turn, CAV2 infection significantly upregulated fluxes through most central metabolism, including glycolysis, pentose-phosphate pathway, glutamine anaplerosis and, more prominently, reductive AKG carboxylation and cytosolic acetyl-coenzyme A formation, suggestive of increased lipogenesis. Based on these results, we suggest culture supplementation strategies to stimulate nucleic acid and lipid biosynthesis for improved canine adenoviral vector production.
[Mh] Termos MeSH primário: Adenovirus Caninos/fisiologia
Glucose/farmacocinética
Glutamina/farmacocinética
Células Madin Darby de Rim Canino/virologia
Análise do Fluxo Metabólico/métodos
[Mh] Termos MeSH secundário: Animais
Isótopos de Carbono/farmacocinética
Proliferação Celular
Transformação Celular Viral
Cães
Regulação da Expressão Gênica
Glicólise
Lipogênese
Células Madin Darby de Rim Canino/metabolismo
Via de Pentose Fosfato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carbon Isotopes); 0RH81L854J (Glutamine); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160324
[St] Status:MEDLINE
[do] DOI:10.1038/srep23529


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[PMID]:27001767
[Au] Autor:Walker D; Abbondati E; Cox AL; Mitchell GB; Pizzi R; Sharp CP; Philbey AW
[Ad] Endereço:Royal (Dick) School of Veterinary Studies and the Roslin Institute, University of Edinburgh, Easter Bush, Edinburgh EH25 9RG, UK.
[Ti] Título:Infectious canine hepatitis in red foxes (Vulpes vulpes) in wildlife rescue centres in the UK.
[So] Source:Vet Rec;178(17):421, 2016 Apr 23.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Outbreaks of infectious canine hepatitis are described in red foxes ( ITALIC! Vulpes vulpes) at two wildlife rescue centres in the UK. Disease occurred in two-month-old to four-month-old juvenile foxes, which were held in small enclosures in groups of three to eight animals. The foxes died or were euthanased after a short clinical course, sometimes including neurological signs and jaundice, with a high case fatality rate. Four red foxes submitted for postmortem examination had enlarged, congested livers, with rounded borders and mild accentuation of the lobular pattern. On histological examination, there was random, multifocal to massive hepatic necrosis, along with multifocal vasculitis in the central nervous system (CNS) and mild, multifocal glomerulonephritis. Intranuclear inclusion bodies, typical of canine adenovirus type 1 (CAV-1) infection, were present in hepatocytes, vascular endothelial cells in the CNS, renal glomeruli and renal tubular epithelial cells. CAV-1 was detected in tissues from affected foxes by PCR and sequencing. Congregation of juvenile foxes in wildlife rescue centres is likely to be a risk factor for transmission of CAV-1. Preventive measures in wildlife centres should be implemented to prevent the spread of the virus among conspecifics and to other susceptible species.
[Mh] Termos MeSH primário: Adenovirus Caninos/isolamento & purificação
Animais Selvagens/virologia
Surtos de Doenças/veterinária
Raposas/virologia
Hepatite Infecciosa Canina/diagnóstico
[Mh] Termos MeSH secundário: Animais
DNA Viral
Cães
Feminino
Masculino
Reação em Cadeia da Polimerase/veterinária
Análise de Sequência de DNA/veterinária
Reino Unido/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160323
[St] Status:MEDLINE
[do] DOI:10.1136/vr.103559


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[PMID]:26903420
[Au] Autor:Li Y; Hickey L; Perrins R; Werlen E; Patel AA; Hirschberg S; Jones MW; Salinas S; Kremer EJ; Pickering AE
[Ad] Endereço:School of Physiology & Pharmacology, University of Bristol, Bristol BS8 1TD, UK.
[Ti] Título:Retrograde optogenetic characterization of the pontospinal module of the locus coeruleus with a canine adenoviral vector.
[So] Source:Brain Res;1641(Pt B):274-90, 2016 Jun 15.
[Is] ISSN:1872-6240
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Noradrenergic neurons of the brainstem extend projections throughout the neuraxis to modulate a wide range of processes including attention, arousal, autonomic control and sensory processing. A spinal projection from the locus coeruleus (LC) is thought to regulate nociceptive processing. To characterize and selectively manipulate the pontospinal noradrenergic neurons in rats, we implemented a retrograde targeting strategy using a canine adenoviral vector to express channelrhodopsin2 (CAV2-PRS-ChR2-mCherry). LC microinjection of CAV2-PRS-ChR2-mCherry produced selective, stable, transduction of noradrenergic neurons allowing reliable opto-activation in vitro. The ChR2-transduced LC neurons were opto-identifiable in vivo and functional control was demonstrated for >6 months by evoked sleep-wake transitions. Spinal injection of CAV2-PRS-ChR2-mCherry retrogradely transduced pontine noradrenergic neurons, predominantly in the LC but also in A5 and A7. A pontospinal LC (ps:LC) module was identifiable, with somata located more ventrally within the nucleus and with a discrete subset of projection targets. These ps:LC neurons had distinct electrophysiological properties with shorter action potentials and smaller afterhyperpolarizations compared to neurons located in the core of the LC. In vivo recordings of ps:LC neurons showed a lower spontaneous firing frequency than those in the core and they were all excited by noxious stimuli. Using this CAV2-based approach we have demonstrated the ability to retrogradely target, characterise and optogenetically manipulate a central noradrenergic circuit and show that the ps:LC module forms a discrete unit. This article is part of a Special Issue entitled SI: Noradrenergic System.
[Mh] Termos MeSH primário: Locus Cerúleo/citologia
Locus Cerúleo/fisiologia
Neurônios/citologia
Neurônios/fisiologia
Optogenética
[Mh] Termos MeSH secundário: Adenovirus Caninos/genética
Animais
Cerebelo/citologia
Cerebelo/fisiologia
Vetores Genéticos
Giro do Cíngulo/citologia
Giro do Cíngulo/fisiologia
Masculino
Potenciais da Membrana/fisiologia
Vias Neurais/citologia
Vias Neurais/fisiologia
Técnicas de Rastreamento Neuroanatômico
Norepinefrina/metabolismo
Optogenética/métodos
Substância Cinzenta Periaquedutal/citologia
Substância Cinzenta Periaquedutal/fisiologia
Ratos Long-Evans
Ratos Wistar
Sono/fisiologia
Medula Espinal/citologia
Medula Espinal/fisiologia
Técnicas de Cultura de Tecidos
Vigília/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
X4W3ENH1CV (Norepinephrine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160224
[St] Status:MEDLINE


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PubMed Central Texto completo
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[PMID]:26822375
[Au] Autor:Curi NH; Massara RL; de Oliveira Paschoal AM; Soriano-Araújo A; Lobato ZI; Demétrio GR; Chiarello AG; Passamani M
[Ad] Endereço:Postgraduate Programme in Applied Ecology, Department of Biology, Federal University of Lavras, Lavras, Brazil. nelsoncuri@hotmail.com.
[Ti] Título:Prevalence and risk factors for viral exposure in rural dogs around protected areas of the Atlantic forest.
[So] Source:BMC Vet Res;12:21, 2016 Jan 28.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Despite the crucial role of domestic dogs as reservoirs for zoonosis and some of the most threatening diseases for wild carnivores such as distemper and parvovirosis, little is known about the epidemiological features and the risk factors involved in pathogen exposure of dogs that live in human/wildlife interfaces and actually contacts wildlife. Through a cross-sectional serological approach and questionnaire survey, we assessed the prevalence along with individual and environment-associated risk factors for four important viral diseases of rural dogs living in households around six Atlantic Forest fragments in southeast Brazil. RESULTS: Widespread exposure to canine parvovirus (97%), canine distemper virus (15%) and canine adenovirus (27%) was detected, but none for canine coronavirus. Dogs from small private reserves were more exposed to parvovirus and canine distemper virus than those from larger state parks. Exposure was associated with dog sex and age, lack of health care and the number of people in the households. Remarkably, factors linked to free-ranging behaviour of dogs were associated with the exposure for all pathogens detected. CONCLUSIONS: According to identified associations, reducing viral pathogen exposure in dogs will require inhibiting dog's movements and access to nearby forests and villages and improving veterinary assistance. Promoting dog vaccination and population control through sterilization around protected areas is also necessary. The study provides support for preventive management actions aimed to protect the health of rural dogs, and consequently of Atlantic Forest's wild carnivores.
[Mh] Termos MeSH primário: Doenças do Cão/epidemiologia
Doenças do Cão/virologia
Viroses/veterinária
[Mh] Termos MeSH secundário: Adenovirus Caninos
Criação de Animais Domésticos
Animais
Brasil/epidemiologia
Vírus da Cinomose Canina
Cães
Feminino
Florestas
Seres Humanos
Masculino
Parvovirus Canino
Animais de Estimação/virologia
Prevalência
Fatores de Risco
Viroses/epidemiologia
Viroses/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160202
[Lr] Data última revisão:
160202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160130
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-016-0646-3


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[PMID]:26589403
[Au] Autor:Millán J; López-Bao JV; García EJ; Oleaga Á; Llaneza L; Palacios V; de la Torre A; Rodríguez A; Dubovi EJ; Esperón F
[Ad] Endereço:Facultad de Ecología y Recursos Naturales, Universidad Andres Bello, República 252, Santiago, Chile. syngamustrachea@hotmail.com.
[Ti] Título:Patterns of Exposure of Iberian Wolves (Canis lupus) to Canine Viruses in Human-Dominated Landscapes.
[So] Source:Ecohealth;13(1):123-34, 2016 Mar.
[Is] ISSN:1612-9210
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wildlife inhabiting human-dominated landscapes is at risk of pathogen spill-over from domestic species. With the aim of gaining knowledge in the dynamics of viral infections in Iberian wolves (Canis lupus) living in anthropized landscapes of northern Spain, we analysed between 2010 and 2013 the samples of 54 wolves by serology and polymerase chain reaction (PCR) for exposure to four pathogenic canine viruses: canine distemper virus (CDV), canine parvovirus-2 (CPV), canine adenovirus 1 and 2 (CAV-1 and CAV-2) and canine herpesvirus. Overall, 76% of the studied wolves presented evidence of exposure to CPV (96% by HI, 66% by PCR) and 75% to CAV (75% by virus neutralization (VN), 76% by PCR, of which 70% CAV-1 and 6% CAV-2). This represents the first detection of CAV-2 infection in a wild carnivore. CPV/CAV-1 co-infection occurred in 51% of the wolves. The probability of wolf exposure to CPV was positively and significantly correlated with farm density in a buffer zone around the place where the wolf was found, indicating that rural dogs might be the origin of CPV infecting wolves. CPV and CAV-1 appear to be enzootic in the Iberian wolf population, which is supported by the absence of seasonal and inter-annual variations in the proportion of positive samples detected. However, while CPV may depend on periodical introductions by dogs, CAV-1 may be maintained within the wolf population. All wolves were negative for exposure to CDV (by VN and PCR) and CHV (by PCR). The absence of acquired immunity against CDV in this population may predispose it to an elevated rate of mortality in the event of a distemper spill-over via dogs.
[Mh] Termos MeSH primário: Adenovirus Caninos/isolamento & purificação
Vírus da Cinomose Canina/isolamento & purificação
Herpesvirus Canídeo 1/isolamento & purificação
Parvovirus Canino/isolamento & purificação
Lobos/virologia
[Mh] Termos MeSH secundário: Animais
Animais Selvagens
Coinfecção
Cães/virologia
Seres Humanos
Reação em Cadeia da Polimerase
Fatores de Risco
Testes Sorológicos/veterinária
Espanha
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151122
[St] Status:MEDLINE
[do] DOI:10.1007/s10393-015-1074-8


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[PMID]:26181626
[Au] Autor:Simão D; Pinto C; Fernandes P; Peddie CJ; Piersanti S; Collinson LM; Salinas S; Saggio I; Schiavo G; Kremer EJ; Brito C; Alves PM
[Ad] Endereço:iBET-Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal.
[Ti] Título:Evaluation of helper-dependent canine adenovirus vectors in a 3D human CNS model.
[So] Source:Gene Ther;23(1):86-94, 2016 Jan.
[Is] ISSN:1476-5462
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Gene therapy is a promising approach with enormous potential for treatment of neurodegenerative disorders. Viral vectors derived from canine adenovirus type 2 (CAV-2) present attractive features for gene delivery strategies in the human brain, by preferentially transducing neurons, are capable of efficient axonal transport to afferent brain structures, have a 30-kb cloning capacity and have low innate and induced immunogenicity in preclinical tests. For clinical translation, in-depth preclinical evaluation of efficacy and safety in a human setting is primordial. Stem cell-derived human neural cells have a great potential as complementary tools by bridging the gap between animal models, which often diverge considerably from human phenotype, and clinical trials. Herein, we explore helper-dependent CAV-2 (hd-CAV-2) efficacy and safety for gene delivery in a human stem cell-derived 3D neural in vitro model. Assessment of hd-CAV-2 vector efficacy was performed at different multiplicities of infection, by evaluating transgene expression and impact on cell viability, ultrastructural cellular organization and neuronal gene expression. Under optimized conditions, hd-CAV-2 transduction led to stable long-term transgene expression with minimal toxicity. hd-CAV-2 preferentially transduced neurons, whereas human adenovirus type 5 (HAdV5) showed increased tropism toward glial cells. This work demonstrates, in a physiologically relevant 3D model, that hd-CAV-2 vectors are efficient tools for gene delivery to human neurons, with stable long-term transgene expression and minimal cytotoxicity.
[Mh] Termos MeSH primário: Adenovirus Caninos/genética
Sistema Nervoso Central/metabolismo
Vetores Genéticos
Transdução Genética
[Mh] Termos MeSH secundário: Adenovírus Humanos/genética
Animais
Transporte Axonal
Diferenciação Celular
Sobrevivência Celular
Clonagem Molecular
Modelos Animais de Doenças
Regulação da Expressão Gênica
Técnicas de Transferência de Genes
Terapia Genética
Seres Humanos
Células-Tronco Neurais/citologia
Células-Tronco Neurais/metabolismo
Neuroglia/metabolismo
Neurônios/citologia
Neurônios/metabolismo
Transgenes
Tropismo Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150717
[St] Status:MEDLINE
[do] DOI:10.1038/gt.2015.75



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