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[PMID]:27775992
[Au] Autor:Ma WS; Ma JG; Xing LN
[Ad] Endereço:Department of Tumor Radiotherapy, the Second Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
[Ti] Título:Efficacy and safety of recombinant human adenovirus p53 combined with chemoradiotherapy in the treatment of recurrent nasopharyngeal carcinoma.
[So] Source:Anticancer Drugs;28(2):230-236, 2017 02.
[Is] ISSN:1473-5741
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study aims to explore the efficacy and safety of recombinant human adenovirus p53 (rAd-p53) combined with chemoradiotherapy (CRT) in the treatment of recurrent nasopharyngeal carcinoma (NPC). A total of 162 recurrent NPC patients were selected and divided randomly into the rAd-p53+CRT, CRT, and rAd-p53 groups. An electrochemical luminescence immune analyzer was used to detect serum levels of tumor markers (carcinoembryonic antigen, cancer antigen 199, and cancer antigen 153). Efficacy evaluation was in accordance with Response Evaluation Criteria in Solid Tumor. Toxicity evaluation was performed according to the WHO grading standard. A 3-year follow-up was performed. A Kaplan-Meier curve was drawn to calculate progression-free survival and the 3-year survival rate. The complete response rate and effective rate (complete response+partial response) of recurrent NPC patients in the rAd-p53+CRT group were higher than those in the CRT and rAd-p53groups. After treatment, compared with the CRT and rAd-p53 groups, the rAd-p53+CRT group had lower serum levels of carcinoembryonic antigen, cancer antigen 199, and cancer antigen 153. The incidences of leukopenia and oral mucositis in the rAd-p53+CRT group were lower than those in the CRT group, but no differences were found between the rAd-p53+CRT and rAd-p53 groups. The progression-free survival and 3-year survival rate of recurrent NPC patients in the rAd-p53+CRT group were higher than the than those in the CRT and rAd-p53 groups. Our study supports that rAd-p53 combined with CRT may provide better efficacy and lower toxicity than rAd-p53 or CRT alone for the treatment of recurrent NPC patients.
[Mh] Termos MeSH primário: Adenovírus Humanos/genética
Neoplasias Nasofaríngeas/terapia
Proteína Supressora de Tumor p53/genética
[Mh] Termos MeSH secundário: Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico
Biomarcadores Tumorais/sangue
Quimiorradioterapia
Cisplatino/administração & dosagem
Terapia Combinada
Feminino
Fluoruracila/administração & dosagem
Seres Humanos
Masculino
Meia-Idade
Neoplasias Nasofaríngeas/sangue
Neoplasias Nasofaríngeas/virologia
Recidiva Local de Neoplasia/sangue
Recidiva Local de Neoplasia/terapia
Recidiva Local de Neoplasia/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (TP53 protein, human); 0 (Tumor Suppressor Protein p53); Q20Q21Q62J (Cisplatin); U3P01618RT (Fluorouracil)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1097/CAD.0000000000000448


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[PMID]:29225167
[Au] Autor:Jehung JP; Kitamura T; Yanagawa-Matsuda A; Kuroshima T; Towfik A; Yasuda M; Sano H; Kitagawa Y; Minowa K; Shindoh M; Higashino F
[Ad] Endereço:Department of Restorative Dentistry, Hokkaido University, Faculty of Dental Medicine, Graduate School of Dental Medicine, Sapporo, Japan.
[Ti] Título:Adenovirus infection induces HuR relocalization to facilitate virus replication.
[So] Source:Biochem Biophys Res Commun;495(2):1795-1800, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:HuR is an RNA-binding protein of the embryonic lethal abnormal vision (ELAV) family, which binds to the AU-rich element (ARE) in the 3'-untranslated region (UTR) of certain mRNAs and is involved in the nucleo-cytoplasmic export and stabilization of ARE-mRNAs. The cytoplasmic relocalization of ARE-mRNAs with several proteins such as HuR and pp32 increases in cells transformed by the adenovirus oncogene product E4orf6. Additionally, these ARE-mRNAs were stabilized and acquired the potential to transform cells. Although, the relocalization of HuR and the stabilization of ARE-mRNAs are crucial for cell transformation, evidence regarding the relationship of HuR and ARE-mRNAs with adenovirus replication is lacking. In this report, we demonstrate that adenovirus infection induces the relocation of HuR to the cytoplasm of host cells. Analysis using the luciferase-ARE fusion gene and the tetracycline (tet)-off system revealed that the process of stabilizing ARE-mRNAs is activated in adenovirus-infected cells. Heat shock treatment or knockdown-mediated depletion of HuR reduced adenovirus production. Furthermore, expression of ARE-including viral IVa2 mRNA, decreased in HuR-depleted infected cells. These results indicate that HuR plays an important role in adenovirus replication, at least in part, by up-regulating IVa2 mRNA expression and that ARE-mRNA stabilization is required for both transformation and virus replication.
[Mh] Termos MeSH primário: Infecções por Adenovirus Humanos/metabolismo
Infecções por Adenovirus Humanos/virologia
Proteína Semelhante a ELAV 1/metabolismo
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas
Elementos Ricos em Adenilato e Uridilato
Infecções por Adenovirus Humanos/genética
Adenovírus Humanos/genética
Adenovírus Humanos/fisiologia
Transformação Celular Viral/genética
Proteína Semelhante a ELAV 1/antagonistas & inibidores
Proteína Semelhante a ELAV 1/genética
Técnicas de Silenciamento de Genes
Células HeLa
Seres Humanos
Transporte Proteico
Estabilidade de RNA
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Proteínas Virais/genética
Replicação Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (ELAV-Like Protein 1); 0 (ELAVL1 protein, human); 0 (RNA, Messenger); 0 (Viral Proteins); 0 (iva2 protein, adenovirus)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE


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[PMID]:28455139
[Au] Autor:Kocazeybek B; Dinc HO; Ergin S; Saribas S; Ozcabi BT; Cizmecigil U; Altan E; Atalik K; Yüksel P; Taner Z; Karakullukcu A; Sirekbasan S; Turan N; Cagatay P; Imamova N; Evliyaoglu O; Yilmaz H
[Ad] Endereço:Istanbul University, Cerrahpasa Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey. Electronic address: bzeybek@istanbul.edu.tr.
[Ti] Título:Evaluation of Adenovirus-36 (Ad-36) antibody seropositivity and adipokine levels in obese children.
[So] Source:Microb Pathog;108:27-31, 2017 Jul.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Adenovirus 36 (Ad-36) has recently been suggested as a possible contributor to the current obesity epidemic. The aim of this study was to investigate the prevalence of Ad-36 antibodies in obese children, as well as investigate the role of serum leptin and lipid levels in Ad-36-obesity. Seventy-one obese children and 62 non-obese children were included as the patient group (PG), including the healthy control group (HCG), respectively. Simultaneously, Ad-36 antibodies and adipokine levels were assessed with serum neutralization assays (SNA) and ELISA. Ad-36 antibody was detected in 9 patients (12.7%) and 1 patient (1.6%) in both the PG and HCG, respectively, while a significant difference was detected between groups (p < 0.05). Although serum LDL, total cholesterol, triglycerides and leptin levels were detected significantly higher, adiponectin level was detected paradoxically lower in the PG. However, a significant difference was not detected for lipids and leptin levels; adiponectin levels were found to be significantly lower in Ad-36 antibody-positive PG (p < 0.05). In conclusion, we suggest there is an association between Ad-36 and obesity in children, including IL-6 levels increasing in obese children with Ad-36 seropositivity. Conversely, adiponectin levels in obese children with Ad-36 seropositivity were higher. As such, there is a need for studies to understand the mechanisms underlying this observation.
[Mh] Termos MeSH primário: Adenovírus Humanos/imunologia
Adipocinas/sangue
Anticorpos Antivirais/sangue
Obesidade/sangue
Obesidade/epidemiologia
Obesidade/virologia
[Mh] Termos MeSH secundário: Infecções por Adenovirus Humanos/complicações
Infecções por Adenovirus Humanos/virologia
Adiponectina/sangue
Adolescente
Índice de Massa Corporal
Estudos de Casos e Controles
Criança
Colesterol/sangue
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Interleucina-6/sangue
Leptina/sangue
Lipídeos/sangue
Masculino
Testes de Neutralização
Prevalência
Fatores de Risco
Triglicerídeos/sangue
Turquia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ADIPOQ protein, human); 0 (Adipokines); 0 (Adiponectin); 0 (Antibodies, Viral); 0 (Interleukin-6); 0 (Leptin); 0 (Lipids); 0 (Triglycerides); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:29240811
[Au] Autor:Wang W; Liu Y; Zhou Y; Gu L; Zhang L; Zhang X; Chen M; Zou Z; Qiu W; Hu X; Fan Q
[Ad] Endereço:Center for Disease Control and Prevention of Chengdu Military Region, Chengdu, China.
[Ti] Título:Whole-genome analyses of human adenovirus type 55 emerged in Tibet, Sichuan and Yunnan in China, in 2016.
[So] Source:PLoS One;12(12):e0189625, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three outbreaks of acute respiratory disease occurred at military camps in 2016 at Tibet, Sichuan and Yunnan province, China. The pathogen induced these three outbreaks were all confirmed as HAdV-55 by genotype-specific PCR. The outbreak in Tibet was the first report that HAdV-55 occurred in the high altitude (HA, above sea level 3658 m). This study aims to determine the gene variation and evolution characteristics of these viral strains. Three strains of adenoviruses, LS89/Tibet/2016 (GenBank accession no. KY002683), SF04/SC/2016 (GenBank accession no. KY002684) and KM03/YN/2016 (GenBank accession no. KY002685) were obtained and confirmed by wholegenome sequencing. No multi-gene fragments recombination were found in these isolated HAdV-55 virus compared with previous reported HAdV-55 strains in China. The outbreaks in Tibet and in Sichuan continuously occurred. Virus isolated from Tibet (LS89/Tibet/2016) and Sichuan (SF04/SC/2016) had a similar mutation pattern and had a closer genetic evolutionary distance than KM03/YN/2016 strain, which indicates that the pathogens causing these two outbreaks may be of the same origin. Moreover, we found that heating was an effective way to inactive these viruses, which provide valuable information for the development of HAdV-55 vaccines. Our data provide new information for genetic evolution of HAdV-55, and contribute to the prevention and control of HAdV-55 infection in the future.
[Mh] Termos MeSH primário: Infecções por Adenovirus Humanos/virologia
Adenovírus Humanos/genética
Genoma Viral
Infecções Respiratórias/virologia
[Mh] Termos MeSH secundário: Infecções por Adenovirus Humanos/epidemiologia
Adenovírus Humanos/classificação
Adenovírus Humanos/crescimento & desenvolvimento
Adenovírus Humanos/isolamento & purificação
Linhagem Celular Tumoral
China/epidemiologia
Surtos de Doenças
Seres Humanos
Filogenia
Infecções Respiratórias/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189625


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[PMID]:28747638
[Au] Autor:Hong SS; Marotte H; Courbon G; Firestein GS; Boulanger P; Miossec P
[Ad] Endereço:University of Lyon, Lyon, 69007, France. saw-see.hong@univ-lyon1.fr.
[Ti] Título:PUMA gene delivery to synoviocytes reduces inflammation and degeneration of arthritic joints.
[So] Source:Nat Commun;8(1):146, 2017 07 27.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In rheumatoid arthritis (RA), the proliferation of fibroblast-like synoviocytes (FLS) is the cause of chronic inflammation in joints and of joint damage. Delivery of the pro-apoptotic gene PUMA to FLS via human adenovirus type 5 (HAdV5) vectors has been tested as a therapeutic approach, but efficiency is hampered by low transduction, as FLS do not express HAdV5 receptors on the cell surface. Here we show that efficient transduction of PUMA in FLS can be achieved by conjugating HAdV5 to a baculovirus, which binds to the cell surface via the envelope glycoprotein Gp64. Intra-articular injection in an adjuvant-induced rat model of RA induces apoptosis of FLS, leading to significant decrease in joint inflammation, joint damage, and bone loss with improvement in joint function and mobility. Our results demonstrate the therapeutic potential of PUMA gene therapy as a local treatment in various forms of arthritis in which abnormal FLS proliferation is implicated.Proliferation of synoviocytes contributes to joint damage in rheumatoid arthritis. Here the authors show that targeting of these cells by a vector, consisting of a baculovirus conjugated to an adenovirus carrying the pro-apoptotic gene PUMA, has therapeutic efficacy in a rat arthritis model.
[Mh] Termos MeSH primário: Proteínas Reguladoras de Apoptose/genética
Artrite Reumatoide/genética
Proteínas Proto-Oncogênicas/genética
Sinoviócitos/metabolismo
Sinovite/genética
[Mh] Termos MeSH secundário: Adenovírus Humanos/genética
Animais
Apoptose/genética
Proteínas Reguladoras de Apoptose/metabolismo
Artrite Experimental/genética
Artrite Experimental/metabolismo
Artrite Experimental/terapia
Artrite Reumatoide/terapia
Baculoviridae/genética
Proliferação Celular/genética
Células Cultivadas
Feminino
Técnicas de Transferência de Genes
Terapia Genética/métodos
Vetores Genéticos/genética
Células HEK293
Seres Humanos
Proteínas Proto-Oncogênicas/metabolismo
Ratos Endogâmicos Lew
Sinovite/terapia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins); 0 (BBC3 protein, human); 0 (Proto-Oncogene Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-00142-1


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[PMID]:28981484
[Au] Autor:Binder AM; Biggs HM; Haynes AK; Chommanard C; Lu X; Erdman DD; Watson JT; Gerber SI
[Ad] Endereço:Division of Viral Diseases, National Center for Immunization and Respiratory Disease, CDC.
[Ti] Título:Human Adenovirus Surveillance - United States, 2003-2016.
[So] Source:MMWR Morb Mortal Wkly Rep;66(39):1039-1042, 2017 Oct 06.
[Is] ISSN:1545-861X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human adenoviruses (HAdVs) are nonenveloped, double-stranded DNA viruses in the family Adenoviridae; seven species (A-G) and >60 genotypes are known to cause human infection (1). Clinical manifestations associated with HAdV infection include fever, acute respiratory illness, gastroenteritis, and conjunctivitis. HAdV infection can be severe, particularly among immunocompromised patients, and can cause respiratory failure, disseminated infection, hemorrhagic cystitis, neurologic disease, and death (1,2). Illness tends to occur sporadically and without demonstrated seasonality. Outbreaks of HAdV have been reported globally in communities (3), and in closed or crowded settings, including dormitories, health care settings, and among military recruits, for whom a vaccine against HAdV type 4 (HAdV-4) and HAdV type 7 (HAdV-7) has been developed (4,5). CDC summarized HAdV detections voluntarily reported through the National Adenovirus Type Reporting System (NATRS) after initiation of surveillance in 2014 to describe trends in reported HAdVs circulating in the United States. Reporting laboratories were also encouraged to report available results for specimens collected before surveillance began. Overall, the number of reporting laboratories and HAdV type identifications reported to NATRS has increased substantially from the start of official reporting in 2014 through 2016; this report describes specimens collected during 2003-2016. The most commonly reported HAdV types were HAdV type 3 (HAdV-3) and HAdV type 2 (HAdV-2), although HAdV types reported fluctuated considerably from year to year. In the United States, information on recently circulating HAdV types is needed to inform diagnostic and surveillance activities by clinicians and public health practitioners. Routine reporting to NATRS by all U.S. laboratories with the capacity to type HAdVs could help strengthen this surveillance system.
[Mh] Termos MeSH primário: Infecções por Adenovirus Humanos/epidemiologia
Adenovírus Humanos/isolamento & purificação
Vigilância da População
[Mh] Termos MeSH secundário: Adenovírus Humanos/classificação
Seres Humanos
Estados Unidos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.15585/mmwr.mm6639a2


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[PMID]:28915437
[Au] Autor:Källsten M; Gromova A; Zhao H; Valdés A; Konzer A; Pettersson U; Lind SB
[Ad] Endereço:Department of Chemistry-BMC, Analytical Chemistry, Box 599, Uppsala University, 751 24 Uppsala, Sweden.
[Ti] Título:Temporal characterization of the non-structural Adenovirus type 2 proteome and phosphoproteome using high-resolving mass spectrometry.
[So] Source:Virology;511:240-248, 2017 Nov.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The proteome and phosphoproteome of non-structural proteins of Adenovirus type 2 (Ad2) were time resolved using a developed mass spectrometry approach. These proteins are expressed by the viral genome and important for the infection process, but not part of the virus particle. We unambiguously confirm the existence of 95% of the viral proteins predicted to be encoded by the viral genome. Most non-structural proteins peaked in expression at late time post infection. We identified 27 non-redundant sites of phosphorylation on seven different non-structural proteins. The most heavily phosphorylated protein was the DNA binding protein (DBP) with 15 different sites. The phosphorylation occupancy rate could be calculated and monitored with time post infection for 15 phosphorylated sites on various proteins. In the DBP, phosphorylations with time-dependent relation were observed. The findings show the complexity of the Ad2 non-structural proteins and opens up a discussion for potential new drug targets.
[Mh] Termos MeSH primário: Adenovírus Humanos/crescimento & desenvolvimento
Regulação Viral da Expressão Gênica
Fosfoproteínas/análise
Proteoma/análise
Proteínas não Estruturais Virais/análise
[Mh] Termos MeSH secundário: Linhagem Celular
Fibroblastos/virologia
Seres Humanos
Espectrometria de Massas
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phosphoproteins); 0 (Proteome); 0 (Viral Nonstructural Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE


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[PMID]:28915257
[Au] Autor:Lei Z; Zhu Z; Wang BMC; Mei H; Li H; Ga DZG; Jie G; Chi MMB; Zhang S; Ma C; Xu W
[Ad] Endereço:School of Public Health, Shaanxi University of Chinese Medicine, Xianyang city, Shaanxi province, People's Republic of China.
[Ti] Título:Outbreaks of epidemic keratoconjunctivitis caused by human adenovirus type 8 in the Tibet Autonomous Region of China in 2016.
[So] Source:PLoS One;12(9):e0185048, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:From April to November 2016, two outbreaks of epidemic keratoconjunctivitis (EKC) occurred successively at primary and middle schools in the Tibet Autonomous Region of China, and a total of 197 clinically diagnosed cases were reported. Real-time PCR analyses confirmed that human adenovirus (HAdV) infection was related to these outbreaks. Further studies involving sequence determination and phylogenetic analysis based on the penton base, hexon, and fiber genes indicated that human adenovirus type 8 (HAdV-8), belonging to species D, was responsible for the outbreaks. This is the first report of a HAdV-8 associated EKC outbreak in mainland of China, and the results of this study are expected to provide support for future research into HAdV-8 in China.
[Mh] Termos MeSH primário: Infecções por Adenovirus Humanos/epidemiologia
Adenovírus Humanos
Conjuntivite Viral
Surtos de Doenças
[Mh] Termos MeSH secundário: Linhagem Celular
Conjuntivite Viral/epidemiologia
Conjuntivite Viral/virologia
Feminino
Seres Humanos
Masculino
Tibet/epidemiologia
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185048


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[PMID]:28854799
[Au] Autor:Nikolaeva-Glomb L; Mukova L; Nikolova N; Kussovski V; Doumanova L; Mantareva V; Angelov I; Wöhrle D; Galabov AS
[Ti] Título:Photodynamic Effect of some Phthalocyanines on Enveloped and Naked Viruses.
[So] Source:Acta Virol;61(3):341-346, 2017.
[Is] ISSN:0001-723X
[Cp] País de publicação:Slovakia
[La] Idioma:eng
[Ab] Resumo:Activity of three photosensitizing phthalocyanine derivatives was tested for photodynamic inactivation towards two coated and two non-enveloped viruses - bovine viral diarrhea virus (BVDV), influenza virus A(H3N2), poliovirus type 1 (PV-1) and human adenovirus type 5 (HAdV5). In the case of coated viruses, a combination of virucidal and irradiation effects was registered by octa-methylpyridyloxy-substituted Ga phthalocyanine (Ga8) toward BVDV, whereas tetra-methylpyridyloxy-substituted Ga phthalocyanine (Ga4) revealed a marked photoinactivation only. No such effect was observed towards influenza A virus. In contrast, the photoinactivating potential of Ga4 and Ga8 marked very high values on naked viruses, especially on HAdV5, at which both the virucidal as well as the irradiation effects became combined.
[Mh] Termos MeSH primário: Adenovírus Humanos/efeitos dos fármacos
Vírus da Diarreia Viral Bovina/efeitos dos fármacos
Indóis/farmacologia
Vírus da Influenza A Subtipo H3N2/efeitos dos fármacos
Fármacos Fotossensibilizantes/farmacologia
Poliovirus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Bovinos
Linhagem Celular
Cães
Seres Humanos
Células Madin Darby de Rim Canino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoles); 0 (Photosensitizing Agents); V5PUF4VLGY (phthalocyanine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.4149/av_2017_313


  10 / 6903 MEDLINE  
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[PMID]:28839112
[Au] Autor:Kamel W; Akusjärvi G
[Ad] Endereço:Department of Medical Biochemistry and Microbiology, BMC, Uppsala University, SE-751 23 Uppsala, Sweden.
[Ti] Título:An Ago2-associated capped transcriptional start site small RNA suppresses adenovirus DNA replication.
[So] Source:RNA;23(11):1700-1711, 2017 Nov.
[Is] ISSN:1469-9001
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Here we show that the adenovirus major late promoter produces a 31-nucleotide transcriptional start site small RNA (MLP-TSS-sRNA) that retains the 7-methylguanosine (m7G)-cap and is incorporated onto Ago2-containing RNA-induced silencing complexes (RISC) in human adenovirus-37 infected cells. RNA polymerase II CLIP (UV-cross linking immunoprecipitation) experiments suggest that the MLP-TSS-sRNA is produced by promoter proximal stalling/termination of RNA polymerase II transcription at the site of the small RNA 3' end. The MLP-TSS-sRNA is highly stable in cells and functionally active, down-regulating complementary targets in a sequence and dose-dependent manner. The MLP-TSS-sRNA is transcribed from the opposite strand to the adenoviral DNA polymerase and preterminal protein mRNAs, two essential viral replication proteins. We show that the MLP-TSS-sRNA act in to reduce DNA polymerase and preterminal protein mRNA expression. As a consequence of this, the MLP-TSS-sRNA has an inhibitory effect on the efficiency of viral DNA replication. Collectively, our results suggest that this novel sRNA may serve a regulatory function controlling viral genome replication during a lytic and/or persistent adenovirus infection in its natural host.
[Mh] Termos MeSH primário: Adenovírus Humanos/genética
Adenovírus Humanos/metabolismo
Proteínas Argonauta/metabolismo
Replicação do DNA/genética
RNA Viral/genética
RNA Viral/metabolismo
Replicação Viral/genética
[Mh] Termos MeSH secundário: Proteínas Argonauta/genética
Linhagem Celular
Genes Virais
Células HEK293
Células HeLa
Seres Humanos
Conformação de Ácido Nucleico
Regiões Promotoras Genéticas
Capuzes de RNA/química
Capuzes de RNA/genética
Capuzes de RNA/metabolismo
Estabilidade de RNA
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Viral/química
Complexo de Inativação Induzido por RNA/genética
Complexo de Inativação Induzido por RNA/metabolismo
Sítio de Iniciação de Transcrição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Argonaute Proteins); 0 (EIF2C2 protein, human); 0 (RNA Caps); 0 (RNA, Messenger); 0 (RNA, Viral); 0 (RNA-Induced Silencing Complex)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.1261/rna.061291.117



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