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[PMID]:27810445
[Au] Autor:Jones TH; Muehlhauser V
[Ad] Endereço:Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C & E Trail, Lacombe, Alberta T4L 1W1, Canada. Electronic address: Tineke.Jones@agr.gc.ca.
[Ti] Título:F-coliphages, porcine adenovirus and porcine teschovirus as potential indicator viruses of fecal contamination for pork carcass processing.
[So] Source:Int J Food Microbiol;241:237-243, 2017 Jan 16.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:There are concerns about the zoonotic transmission of viruses through undercooked pork products. There is a lack of information on suitable indicator viruses for fecal contamination with pathogenic enteric viruses in the meat processing chain. The study compared the incidence and levels of contamination of hog carcasses with F-coliphages, porcine teschovirus (PTV), and porcine adenovirus (PAdV) at different stages of the dressing process to assess their potential as indicator viruses of fecal contamination. One hundred swab samples (200cm ) were collected from random sites on hog carcasses at 4 different stages of the dressing process and from retail pork over the span of a year from 2 pork processing plants (500/plant). Viable F-coliphages, PAdV DNA and PTV RNA were each detected on ≥99% of the incoming carcasses at both plants and were traceable through the pork processing chain. Significant correlations were observed between viable F-coliphages and PAdV DNA and between F-coliphages and PTV RNA but not between PAdV DNA and PTV RNA at the various stages of pork processing. Detection of viable F-coliphages was more sensitive than genomic copies of PAdV and PTV at low levels of contamination, making F-coliphages a preferred indicator in the pork slaughter process as it also provides an indication of infectivity. For plant A, F-RNA coliphages were detected in 25%, 63%, and 21% of carcass swabs after pasteurization, evisceration, and retail pork products, respectively. For plant B, F-coliphages were detected in 33%, 25%, and 13% of carcass swabs after skinning, evisceration, and retail pork samples, respectively. Viable F-RNA coliphages were genotyped. Viable F-RNA GII and GIII were generally not detected at the earlier stages of the slaughter process but they were detected on 13% of carcasses after evisceration and 2% of retail pork samples at plant A, which raises concerns of potential food handler contamination during pork processing. Consumers could be at risk when consuming undercooked meat contaminated with pathogenic enteric viruses.
[Mh] Termos MeSH primário: Adenovirus Suínos/isolamento & purificação
Colífagos/isolamento & purificação
Fezes/virologia
Contaminação de Alimentos/análise
Carne/virologia
Teschovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Adenovirus Suínos/genética
Animais
Colífagos/genética
Manipulação de Alimentos
Suínos
Teschovirus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161105
[St] Status:MEDLINE


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[PMID]:26033117
[Au] Autor:Makadiya N; Gaba A; Tikoo SK
[Ad] Endereço:1​ VIDO-InterVac, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E3 Canada 2​ Veterinary Microbiology, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E3 Canada.
[Ti] Título:Cleavage of bovine adenovirus type 3 non-structural 100K protein by protease is required for nuclear localization in infected cells but is not essential for virus replication.
[So] Source:J Gen Virol;96(9):2749-63, 2015 Sep.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The L6 region of bovine adenovirus type 3 (BAdV-3) encodes a non-structural protein named 100K. Rabbit antiserum raised against BAdV-3 100K recognized a protein of 130 kDa at 12-24 h and proteins of 130, 100, 95 and 15 kDa at 36-48 h after BAdV-3 infection. The 100K species localized to the nucleus and the cytoplasm of BAdV-3-infected cells. In contrast, 100K localized predominantly to the cytoplasm of the transfected cells. However, BAdV-3 infection of cells transfected with 100K-enhanced yellow fluorescent protein-expressing plasmid detected fluorescent protein in the nucleus of the cells, suggesting that other viral proteins may be required for the nuclear localization of 100K. Interaction of BAdV-3 100K with BAdV-3 33K protein did not alter the cytoplasmic localization of 100K. However, co-expression of BAdV-3 100K and BAdV-3 protease localized 100K to the nucleolus of the transfected cells. Subsequent analysis suggested that BAdV-3 protease cleaves 100K at two identified potential protease cleavage sites (aa 740-745 and 781-786) in transfected or BAdV-3-infected cells. The cleaved C terminus (107 aa) was localized to the nucleolus of the transfected cells. Further analysis suggested that the cleaved C terminus contains a bipartite nuclear localization signal and utilizes import receptor importin-α3 of the classical importin-α/ß transport pathway for nuclear transport. Successful isolation of recombinant BAdV-3 expressing mutant 100K (substitution of alanine for glycine in the potential protease cleavage site) suggested that cytoplasmic cleavage of BAdV-3 100K by adenoviral protease is not essential for virus replication.
[Mh] Termos MeSH primário: Infecções por Adenoviridae/veterinária
Adenovirus Suínos/fisiologia
Doenças dos Bovinos/virologia
Nucléolo Celular/virologia
Peptídeo Hidrolases/metabolismo
Proteínas não Estruturais Virais/metabolismo
Replicação Viral
[Mh] Termos MeSH secundário: Infecções por Adenoviridae/virologia
Adenovirus Suínos/enzimologia
Adenovirus Suínos/genética
Animais
Bovinos
Linhagem Celular
Peptídeo Hidrolases/genética
Processamento de Proteína Pós-Traducional
Proteínas não Estruturais Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Nonstructural Proteins); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150910
[Lr] Data última revisão:
150910
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150603
[St] Status:MEDLINE
[do] DOI:10.1099/vir.0.000205


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[PMID]:26011074
[Au] Autor:Zhang P; Du E; Ma J; Wang W; Zhang L; Tikoo SK; Yang Z
[Ad] Endereço:College of Veterinary Medicine, North-west A&F University, Yangling, Shaanxi, China.
[Ti] Título:A novel and simple method for rapid generation of recombinant porcine adenoviral vectors for transgene expression.
[So] Source:PLoS One;10(5):e0127958, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many human (different serotypes) and nonhuman adenovirus vectors are being used for gene delivery. However, the current system for isolating recombinant adenoviral vectors is either time-consuming or expensive, especially for the generation of recombinant non-human adenoviral vectors. We herein report a new and simple cloning approach for the rapid generation of a porcine adenovirus (PAdV-3) vector which shows promise for gene transfer to human cells and evasion of human adenovirus type 5 (HAdV-5) immunity. Based on the final cloning plasmid, pFPAV3-CcdB-Cm, and our modified SLiCE strategy (SLiCE cloning and lethal CcdB screening), the process for generating recombinant PAdV-3 plasmids required only one step in 3 days, with a cloning efficiency as high as 620 ± 49.56 clones/ng and zero background (100% accuracy). The recombinant PAdV-3 plasmids could be successfully rescued in porcine retinal pigment epithelium cells (VR1BL), which constitutively express the HAdV-5 E1 and PAdV-3 E1B 55k genes, and the foreign genes were highly expressed at 24 h after transduction into swine testicle (ST) cells. In conclusion, this strategy for generating recombinant PAdV-3 vectors based on our modified SLiCE cloning system was rapid and cost-efficient, which could be used as universal cloning method for modification the other regions of PAdV-3 genome as well as other adenoviral genomes.
[Mh] Termos MeSH primário: Adenovirus Suínos/genética
Técnicas de Transferência de Genes
Vetores Genéticos/metabolismo
Recombinação Genética
Transgenes
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Clonagem Molecular
Genes Reporter
Genoma Viral
Mutagênese Insercional/genética
Mapeamento por Restrição
Sus scrofa
Moldes Genéticos
Transdução Genética
Montagem de Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150617
[Lr] Data última revisão:
150617
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150527
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0127958


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[PMID]:25933160
[Au] Autor:Wilkinson-Ryan I; Kim J; Kim S; Ak F; Dodson L; Colonna M; Powell M; Mutch D; Spitzer D; Hansen T; Goedegebuure SP; Curiel D; Hawkins W
[Ad] Endereço:Washington University School of Medicine, Division of Gynecologic Oncology, St. Louis, United States of America.
[Ti] Título:Incorporation of porcine adenovirus 4 fiber protein enhances infectivity of adenovirus vector on dendritic cells: implications for immune-mediated cancer therapy.
[So] Source:PLoS One;10(5):e0125851, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One strategy in cancer immunotherapy is to capitalize on the key immunoregulatory and antigen presenting capabilities of dendritic cells (DCs). This approach is dependent on efficient delivery of tumor specific antigens to DCs, which subsequently induce an anti-tumor T-cell mediated immune response. Human adenovirus serotype 5 (HAdV5) has been used in human studies for gene delivery, but has limited infection in DCs, which lack the proper receptors. Addition of the porcine fiber knob (PK) from porcine adenovirus type 4 to HAdV5 allows the virus to deliver genetic material via binding to glycosylated surface proteins and bypasses the coxsackie-and-adenovirus receptor required by wild-type HAdV5. In this study we explored the potential therapeutic applications of an adenovirus with PK-based tropism against cancers expressing mesothelin. Infectivity and gene transfer assays were used to compare Ad5-PK to wild-type HAdV5. Mouse models were used to demonstrate peptide specificity and T-cell responses. We show that the PK modification highly augmented infection of DCs, including the CD141+ DC subset, a key subset for activation of naïve CD8+ T-cells. We also show that Ad5-PK increases DC infectivity and tumor specific antigen expression. Finally, vaccination of mice with the Ad5-PK vector resulted in enhanced T-cell-mediated interferon gamma (IFN-γ) release in response to both mesothelin peptide and a tumor line expressing mesothelin. Ad5-PK is a promising tool for cancer immunotherapy as it improves infectivity, gene transfer, protein expression, and subsequent T-cell activation in DCs compared to wild-type HAdV5 viruses.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/uso terapêutico
Terapia Genética
Imunoterapia
Neoplasias/terapia
[Mh] Termos MeSH secundário: Adenovírus Humanos
Adenovirus Suínos/genética
Adenovirus Suínos/imunologia
Animais
Proteínas do Capsídeo/genética
Proteínas do Capsídeo/imunologia
Células Dendríticas/imunologia
Proteínas Ligadas por GPI/biossíntese
Técnicas de Transferência de Genes
Vetores Genéticos
Seres Humanos
Camundongos
Neoplasias/genética
Neoplasias/imunologia
Suínos
Transdução Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (GPI-Linked Proteins); 0 (hexon capsid protein, Adenovirus); 0 (mesothelin)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150502
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0125851


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[PMID]:24960273
[Au] Autor:Jerman UD; Kolenc M; Steyer A; Veranic P; Prijatelj MP; Kreft ME
[Ad] Endereço:Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, SI-1000 Ljubljana, Slovenia. urska.dragin@mf.uni-lj.si.
[Ti] Título:A novel strain of porcine adenovirus detected in urinary bladder urothelial cell culture.
[So] Source:Viruses;6(6):2505-18, 2014 Jun 23.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Contamination of cell cultures is the most common problem encountered in cell culture laboratories. Besides the secondary cell contaminations often occurring in the cell laboratories, the contaminations originating from donor animal or human tissue are equally as common, but usually harder to recognize and as such require special attention. The present study describes the detection of porcine adenovirus (PAdV), strain PAdV-SVN1 in cultures of normal porcine urothelial (NPU) cells isolated from urinary bladders of domestic pigs. NPU cell cultures were evaluated by light microscopy (LM), polymerase chain reaction (PCR), and additionally assessed by transmission electron microscopy (TEM). Characteristic ultrastructure of virions revealed the infection with adenovirus. The adenoviral contamination was further identified by the sequence analysis, which showed the highest similarity to recently described PAdV strain PAdV-WI. Additionally, the cell ultrastructural analysis confirmed the life-cycle characteristic for adenoviruses. To closely mimic the in vivo situation, the majority of research on in vitro models uses cell cultures isolated from human or animal tissue and their subsequent passages. Since the donor tissue could be a potential source of contamination, the microbiological screening of the excised tissue and harvested cell cultures is highly recommended.
[Mh] Termos MeSH primário: Adenovirus Suínos/isolamento & purificação
[Mh] Termos MeSH secundário: Adenovirus Suínos/classificação
Adenovirus Suínos/genética
Adenovirus Suínos/ultraestrutura
Animais
Técnicas de Cultura de Células
Células Cultivadas
Efeito Citopatogênico Viral
DNA Viral
Células Epiteliais/virologia
Genes Virais
Dados de Sequência Molecular
Filogenia
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140625
[St] Status:MEDLINE
[do] DOI:10.3390/v6062505


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[PMID]:24880068
[Au] Autor:Jones TH; Muehlhauser V; Thériault G
[Ad] Endereço:Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C & E Trail, Lacombe, Alberta, Canada T4L 1W1. Electronic address: Tineke.Jones@agr.gc.ca.
[Ti] Título:Comparison of ZetaPlus 60S and nitrocellulose membrane filters for the simultaneous concentration of F-RNA coliphages, porcine teschovirus and porcine adenovirus from river water.
[So] Source:J Virol Methods;206:5-11, 2014 Sep.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Increasing attention is being paid to the impact of agricultural activities on water quality to understand the impact on public health. F-RNA coliphages have been proposed as viral indicators of fecal contamination while porcine teschovirus (PTV) and porcine adenovirus (PAdV) are proposed indicators of fecal contamination of swine origin. Viruses and coliphages are present in water in very low concentrations and must be concentrated to permit their detection. There is little information comparing the effectiveness of the methods for concentrating F-RNA coliphages with concentration methods for other viruses and vice versa. The objective of this study was to compare 5 current published methods for recovering F-RNA coliphages, PTV and PAdV from river water samples concentrated by electronegative nitrocellulose membrane filters (methods A and B) or electropositive Zeta Plus 60S filters (methods C-E). Method A is used routinely for the detection of coliphages (Méndez et al., 2004) and method C (Brassard et al., 2005) is the official method in Health Canada's compendium for the detection of viruses in bottled mineral or spring water. When river water was inoculated with stocks of F-RNA MS2, PAdV, and PTV to final concentrations of 1×10(6) PFU/100 mL, 1×10(5) gc/100 mL and 3×10(5) gc/100 mL, respectively, a significantly higher recovery for each virus was consistently obtained for method A with recoveries of 52% for MS2, 95% for PAdV, and 1.5% for PTV. When method A was compared with method C for the detection of F-coliphages, PAdV and PTV in river water samples, viruses were detected with higher frequencies and at higher mean numbers with method A than with method C. With method A, F-coliphages were detected in 11/12 samples (5-154 PFU/100 mL), PTV in 12/12 samples (397-10,951 gc/100 mL), PAdV in 1/12 samples (15 gc/100 mL), and F-RNA GIII in 1/12 samples (750 gc/100 mL) while F-RNA genotypes I, II, and IV were not detected by qRT-PCR.
[Mh] Termos MeSH primário: Adenovirus Suínos/isolamento & purificação
Levivirus/isolamento & purificação
Rios/virologia
Teschovirus/isolamento & purificação
Poluição da Água
Qualidade da Água
[Mh] Termos MeSH secundário: Adenovirus Suínos/genética
Canadá
Filtração/métodos
Levivirus/genética
Sensibilidade e Especificidade
Teschovirus/genética
Ligação Viral
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1505
[Cu] Atualização por classe:140721
[Lr] Data última revisão:
140721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140601
[St] Status:MEDLINE


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[PMID]:24135674
[Au] Autor:Maunula L; Kaupke A; Vasickova P; Söderberg K; Kozyra I; Lazic S; van der Poel WH; Bouwknegt M; Rutjes S; Willems KA; Moloney R; D'Agostino M; de Roda Husman AM; von Bonsdorff CH; Rzezutka A; Pavlik I; Petrovic T; Cook N
[Ad] Endereço:Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Finland. Electronic address: leena.maunula@helsinki.fi.
[Ti] Título:Tracing enteric viruses in the European berry fruit supply chain.
[So] Source:Int J Food Microbiol;167(2):177-85, 2013 Oct 15.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In recent years, numerous foodborne outbreaks due to consumption of berry fruit contaminated by human enteric viruses have been reported. This European multinational study investigated possible contamination routes by monitoring the entire food chain for a panel of human and animal enteric viruses. A total of 785 samples were collected throughout the food production chain of four European countries (Czech Republic, Finland, Poland and Serbia) during two growing seasons. Samples were taken during the production phase, the processing phase, and at point-of-sale. Samples included irrigation water, animal faeces, food handlers' hand swabs, swabs from toilets on farms, from conveyor belts at processing plants, and of raspberries or strawberries at points-of-sale; all were subjected to virus analysis. The samples were analysed by real-time (reverse transcription, RT)-PCR, primarily for human adenoviruses (hAdV) to demonstrate that a route of contamination existed from infected persons to the food supply chain. The analyses also included testing for the presence of selected human (norovirus, NoV GI, NoV GII and hepatitis A virus, HAV), animal (porcine adenovirus, pAdV and bovine polyomavirus, bPyV) and zoonotic (hepatitis E virus, HEV) viruses. At berry production, hAdV was found in 9.5%, 5.8% and 9.1% of samples of irrigation water, food handlers' hands and toilets, respectively. At the processing plants, hAdV was detected in one (2.0%) swab from a food handler's hand. At point-of-sale, the prevalence of hAdV in fresh raspberries, frozen raspberries and fresh strawberries, was 0.7%, 3.2% and 2.0%, respectively. Of the human pathogenic viruses, NoV GII was detected in two (3.6%) water samples at berry production, but no HAV was detected in any of the samples. HEV-contaminated frozen raspberries were found once (2.6%). Animal faecal contamination was evidenced by positive pAdV and bPyV assay results. At berry production, one water sample contained both viruses, and at point-of-sale 5.7% and 1.3% of fresh and frozen berries tested positive for pAdV. At berry production hAdV was found both in irrigation water and on food handler's hands, which indicated that these may be important vehicles by which human pathogenic viruses enter the berry fruit chain. Moreover, both zoonotic and animal enteric viruses could be detected on the end products. This study gives insight into viral sources and transmission routes and emphasizes the necessity for thorough compliance with good agricultural and hygienic practice at the farms to help protect the public from viral infections.
[Mh] Termos MeSH primário: Contaminação de Alimentos/análise
Manipulação de Alimentos/métodos
Frutas/virologia
[Mh] Termos MeSH secundário: Adenovírus Humanos/isolamento & purificação
Adenovirus Suínos/isolamento & purificação
Irrigação Agrícola
Animais
Bovinos
República Tcheca
Surtos de Doenças
Enterovirus
Fezes/virologia
Finlândia
Abastecimento de Alimentos
Mãos/virologia
Vírus da Hepatite A/isolamento & purificação
Vírus da Hepatite E/isolamento & purificação
Seres Humanos
Norovirus/isolamento & purificação
Polônia
Polyomavirus/isolamento & purificação
Sérvia
Suínos
Vírus
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1403
[Cu] Atualização por classe:131028
[Lr] Data última revisão:
131028
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131019
[St] Status:MEDLINE


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[PMID]:23866141
[Au] Autor:Masclaux FG; Hotz P; Friedli D; Savova-Bianchi D; Oppliger A
[Ad] Endereço:Institute for Work and Health (IST), University of Lausanne and University of Geneva, Rue de la Corniche 2, 1066 Epalinges, Switzerland. frederic.masclaux@unil.ch
[Ti] Título:High occurrence of hepatitis E virus in samples from wastewater treatment plants in Switzerland and comparison with other enteric viruses.
[So] Source:Water Res;47(14):5101-9, 2013 Sep 15.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hepatitis E virus (HEV) is responsible for many enterically transmitted viral hepatitides around the world. It is currently one of the waterborne diseases of global concern. In industrialized countries, HEV appears to be more common than previously thought, even if it is rarely virulent. In Switzerland, seroprevalence studies revealed that HEV is endemic, but no information was available on its environmental spread. The aim of this study was to investigate -using qPCR- the occurrence and concentration of HEV and three other viruses (norovirus genogroup II, human adenovirus-40 and porcine adenovirus) in influents and effluents of 31 wastewater treatment plants (WWTPs) in Switzerland. Low concentrations of HEV were detected in 40 out of 124 WWTP influent samples, showing that HEV is commonly present in this region. The frequency of HEV occurrence was higher in summer than in winter. No HEV was detected in WWTP effluent samples, which indicates a low risk of environmental contamination. HEV occurrence and concentrations were lower than those of norovirus and adenovirus. The autochthonous HEV genotype 3 was found in all positive samples, but a strain of the non-endemic and highly pathogenic HEV genotype I was isolated in one sample, highlighting the possibility of environmental circulation of this genotype. A porcine fecal marker (porcine adenovirus) was not detected in HEV positive samples, indicating that swine are not the direct source of HEV present in wastewater. Further investigations will be necessary to determine the reservoirs and the routes of dissemination of HEV.
[Mh] Termos MeSH primário: Vírus da Hepatite E/isolamento & purificação
Eliminação de Resíduos Líquidos
Águas Residuais/virologia
[Mh] Termos MeSH secundário: Adenovírus Humanos/genética
Adenovírus Humanos/isolamento & purificação
Adenovirus Suínos/genética
Adenovirus Suínos/isolamento & purificação
Animais
Fracionamento Químico
Fezes/virologia
Filtração/métodos
Vírus da Hepatite E/genética
Seres Humanos
Norovirus/genética
Norovirus/isolamento & purificação
Reação em Cadeia da Polimerase
Reprodutibilidade dos Testes
Estações do Ano
Suínos
Suíça
Microbiologia da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Waste Water)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:130902
[Lr] Data última revisão:
130902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130720
[St] Status:MEDLINE


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[PMID]:23456331
[Au] Autor:Jung YD; Ha HS; Park SJ; Oh KB; Im GS; Kim TH; Seong HH; Kim HS
[Ad] Endereço:Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609-735, Korea.
[Ti] Título:Identification and promoter analysis of PERV LTR subtypes in NIH-miniature pig.
[So] Source:Mol Cells;35(2):99-105, 2013 Feb.
[Is] ISSN:0219-1032
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Porcine endogenous retroviruses (PERVs) are integrated into the genomes of all pigs. Since some PERVs can also infect human cells, they represent a potential risk for xenotransplantation involving pig cells or organs. The long terminal repeat (LTR) elements of PERVs show promoter activity that can affect human functional genes; therefore, we examined these elements in this study. We detected several expressed LTRs in the NIH-miniature pig liver, among which we identified 9 different subtypes. When these LTRs were compared, distinct structures that contained several insertion and deletion (INDEL) events and tandem repeats were identified in the U3 region. The transcriptional activity of the 9 LTR subtypes was analyzed in the PK15 porcine cell line and in the HepG2 and Hep3B human liver cell lines, and transcriptional regulation was found to be different in the 3 cell lines. The D LTR subtype was found to have stronger promoter activity than all other types in 4 different human cell lines (HepG2, Hep3B, U251, and 293). Using computational approaches, the D type was shown to contain 4 transcription factor-binding sites distinct from those in the U3 regions of the other subtypes. Therefore, deletion mutants were constructed and examined by a transient transfection luciferase assay. The results of this analysis indicated that the binding site for the Hand1:E47 transcription factor might play a positive role in the transcriptional regulation of PERV LTR subtype D in human liver cell lines.
[Mh] Termos MeSH primário: Adenovirus Suínos/genética
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Sítios de Ligação
Regiões Promotoras Genéticas
Porco Miniatura/genética
Sequências Repetidas Terminais
[Mh] Termos MeSH secundário: Adenovirus Suínos/classificação
Animais
Linhagem Celular
DNA Viral
Regulação Viral da Expressão Gênica
Células HEK293
Coração/virologia
Células Hep G2
Seres Humanos
Fígado/virologia
Mutação
Suínos
Porco Miniatura/virologia
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Basic Helix-Loop-Helix Transcription Factors); 0 (DNA, Viral); 0 (Transcription Factors); 0 (helix-loop-helix protein, eHAND)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:150218
[Lr] Data última revisão:
150218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130305
[St] Status:MEDLINE
[do] DOI:10.1007/s10059-013-2289-6


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[PMID]:23383334
[Au] Autor:Kim JW; Glasgow JN; Nakayama M; Ak F; Ugai H; Curiel DT
[Ad] Endereço:Cancer Biology Division, School of Medicine, Washington University in St. Louis, St. Louis, Missouri, United States of America.
[Ti] Título:An adenovirus vector incorporating carbohydrate binding domains utilizes glycans for gene transfer.
[So] Source:PLoS One;8(2):e55533, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Vectors based on human adenovirus serotype 5 (HAdV-5) continue to show promise as delivery vehicles for cancer gene therapy. Nevertheless, it has become clear that therapeutic benefit is directly linked to tumor-specific vector localization, highlighting the need for tumor-targeted gene delivery. Aberrant glycosylation of cell surface glycoproteins and glycolipids is a central feature of malignant transformation, and tumor-associated glycoforms are recognized as cancer biomarkers. On this basis, we hypothesized that cancer-specific cell-surface glycans could be the basis of a novel paradigm in HAdV-5-based vector targeting. METHODOLOGY/PRINCIPAL FINDINGS: As a first step toward this goal, we constructed a novel HAdV-5 vector encoding a unique chimeric fiber protein that contains the tandem carbohydrate binding domains of the fiber protein of the NADC-1 strain of porcine adenovirus type 4 (PAdV-4). This glycan-targeted vector displays augmented CAR-independent gene transfer in cells with low CAR expression. Further, we show that gene transfer is markedly decreased in cells with genetic glycosylation defects and by inhibitors of glycosylation in normal cells. CONCLUSIONS/SIGNIFICANCE: These data provide the initial proof-of-concept for HAdV-5 vector-mediated gene delivery based on the presence of cell-surface carbohydrates. Further development of this new targeting paradigm could provide targeted gene delivery based on vector recognition of disease-specific glycan biomarkers.
[Mh] Termos MeSH primário: Marcação de Genes/métodos
Técnicas de Transferência de Genes
Terapia Genética/métodos
Vetores Genéticos/genética
Neoplasias/terapia
Polissacarídeos/metabolismo
[Mh] Termos MeSH secundário: Adenovírus Humanos
Adenovirus Suínos/metabolismo
Animais
Western Blotting
Células CHO
Linhagem Celular Tumoral
Cricetinae
Cricetulus
Primers do DNA/genética
Seres Humanos
Neoplasias/genética
Plasmídeos/genética
Polissacarídeos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA Primers); 0 (Polysaccharides)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130206
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0055533



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