Base de dados : MEDLINE
Pesquisa : B04.280.045 [Categoria DeCS]
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  1 / 37 MEDLINE  
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[PMID]:28382574
[Au] Autor:Huang GH; Hou DH; Wang M; Cheng XW; Hu Z
[Ad] Endereço:State Key Laboratory of Virology, Microorganisms & Viruses Culture Collection Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, 430071, China.
[Ti] Título:Genome analysis of Heliothis virescens ascovirus 3h isolated from China.
[So] Source:Virol Sin;32(2):147-154, 2017 Apr.
[Is] ISSN:1995-820X
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:No ascovirus isolated from China has been sequenced so far. Therefore, in this study, we aimed to sequence the genome of Heliothis virescens ascovirus 3h (HvAV-3h) using the 454 pyrosequencing technology. The genome was found to be 190,519-bp long with a G+C content of 45.5%. We also found that it encodes 185 hypothetical open reading frames (ORFs) along with at least 50 amino acids, including 181 ORFs found in other ascoviruses and 4 unique ORFs. Gene-parity plots and phylogenetic analysis revealed a close relationship between HvAV-3h and three other HvAV-3a strains and a distant relationship with Spodoptera frugiperda ascovirus 1a (SfAV-1a), Trichoplusia ni ascovirus 6a (TnAV-6a), and Diadromus pulchellus ascovirus 4a (DpAV-4a). Among the 185 potential genes encoded by the genome, 44 core genes were found in all the sequenced ascoviruses. In addition, 25 genes were found to be conserved in all ascoviruses except DpAV-4a. In the HvAV-3h genome, 24 baculovirus repeat ORFs (bros) were present, and the typical homologous repeat regions (hrs) were absent. This study supplies information important for understanding the conservation and functions of ascovirus genes as well as the variety of ascoviral genomes.
[Mh] Termos MeSH primário: Ascoviridae/genética
Ascoviridae/isolamento & purificação
Lepidópteros/virologia
[Mh] Termos MeSH secundário: Animais
Composição de Bases
China
Análise por Conglomerados
DNA Viral/química
DNA Viral/genética
Ordem dos Genes
Genoma Viral
Fases de Leitura Aberta
Filogenia
Análise de Sequência de DNA
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1007/s12250-016-3929-8


  2 / 37 MEDLINE  
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[PMID]:28218573
[Au] Autor:Asgari S; Bideshi DK; Bigot Y; Federici BA; Cheng XW; Ictv Report Consortium
[Ad] Endereço:1​School of Biological Sciences, The University of Queensland, Brisbane, QLD 4072, Australia.
[Ti] Título:ICTV Virus Taxonomy Profile: Ascoviridae.
[So] Source:J Gen Virol;98(1):4-5, 2017 Jan.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The family Ascoviridae includes viruses with circular dsDNA genomes of 100-200 kbp characterized by oblong enveloped virions of 200-400 nm in length. Ascoviruses mainly infect lepidopteran larvae and are mechanically transmitted by parasitoid wasps in which they may also replicate. Most known members belong to the genus Ascovirus, except one virus, that of the genus Toursvirus, which replicates in both its lepidopteran and parasitoid vector hosts. Ascoviruses cause high mortality among economically important insect pests, thereby controlling insect populations. This is a summary of the current International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Ascoviridae, which is available at www.ictv.global/report/ascoviridae.
[Mh] Termos MeSH primário: Ascoviridae/classificação
[Mh] Termos MeSH secundário: Animais
Ascoviridae/genética
Ascoviridae/fisiologia
Ascoviridae/ultraestrutura
Insetos/virologia
Larva/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000677


  3 / 37 MEDLINE  
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[PMID]:27551150
[Au] Autor:Hu J; Wang X; Zhang Y; Zheng Y; Zhou S; Huang GH
[Ad] Endereço:Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Changsha 410128, China (cynthia910830@qq.com; wangxing@hunau.edu.cn; ghhuang@hunau.edu.cn) Institute of Virology, Hunan Agricultural University, Changsha 410128, China College of Plant Protection, Hunan Agric
[Ti] Título:Characterization and Growing Development of Spodoptera exigua (Lepidoptera: Noctuidae) Larvae Infected by Heliothis virescens ascovirus 3h (HvAV-3h).
[So] Source:J Econ Entomol;109(5):2020-6, 2016 Oct.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Spodoptera exigua (Hübner) is a very serious worldwide pest capable of causing severe economic losses in numerous agricultural crops. The need for an effective, highly virulent, pathogenic microorganism for use as a biological control agent against S. exigua larvae is particularly important. Heliothis virescens ascovirus 3 h (HvAV-3h)-containing hemolymph with a titer of 9.58 × 10(12) genome copies per ml was used to inoculate S. exigua larvae per os with a 1.06 × 10(10) dosage per larva for the first- to second instar and 9.58 × 10(9) genome copies per larva for the third- to fifth instars. Intrahemocoelic injections were also used with a dosage of 1.53 × 10(9) genome copies per larva for third- to fifth instar. The postinjection mortality, body weight, and food intake of the S. exigua larvae were observed and recorded. The corrected mortality rates for the first- through fifth instar inoculated per os were 21.88 ± 0.98, 22.22 ± 4.00, 8.89 ± 4.01, 6.66 ± 3.33, and 8.89 ± 2.94%, respectively. The early instars were significantly easier to infect with virus compared to the later instar. The corrected mortality of the third, fourth, and fifth instars inoculated by injection was 96.58 ± 3.42, 98.83 ± 1.17, and 97.78 ± 2.22%, respectively. Compared to the healthy larval population, survival time of the diseased larval population was considerably extended. In addition, food intake was greatly reduced, and the body weight remained fairly constant in the third- and fourth instar. The body weight declined in the fifth instar corresponding to a reduction in food intake.
[Mh] Termos MeSH primário: Ascoviridae/fisiologia
Agentes de Controle Biológico/farmacologia
Spodoptera/crescimento & desenvolvimento
Spodoptera/virologia
[Mh] Termos MeSH secundário: Animais
Larva/crescimento & desenvolvimento
Larva/virologia
Controle Biológico de Vetores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Control Agents)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160824
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tow183


  4 / 37 MEDLINE  
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[PMID]:26878829
[Au] Autor:Li SJ; Hopkins RJ; Zhao YP; Zhang YX; Hu J; Chen XY; Xu Z; Huang GH
[Ad] Endereço:Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, China.
[Ti] Título:Imperfection works: Survival, transmission and persistence in the system of Heliothis virescens ascovirus 3h (HvAV-3h), Microplitis similis and Spodoptera exigua.
[So] Source:Sci Rep;6:21296, 2016 Feb 16.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ascoviruses are insect-specific large DNA viruses that mainly infect noctuid larvae, and are transmitted by parasitoids in the fields. Heliothis virescens ascovirus 3h (HvAV-3h) has been recently isolated from Spodoptera exigua, without parasitoid vector identified previously. Here we report that Microplitis similis, a solitary endoparasitoid wasp, could transmit HvAV-3h between S. exigua larvae in the laboratory. When the female parasitoid wasp acquired the virus and served as a vector, the period of virion viability on the ovipositor was 4.1 ± 1.4 days. Infected host larvae were still acceptable for egg laying by parasitoids, and the parasitoids thereafter transmitted virus to healthy hosts. Virus acquisition occurred only from donor hosts between 3 and 9 days post infection. The peak of virus acquisition (80.9 ± 6.3%) was found when M. similis wasps oviposited in larvae that had been inoculated with the virus 7 days previously. When virus infection of the host took place during the life cycle of the parasitoid wasp, it caused 1- to 4-day-old immature parasitoids death in the host, whilst a small proportion of 5- to 6-day-old and the majority of 7-day-old parasitoids larvae survived from the virus-infected hosts. Viral contamination did not reduce the life span or fecundity of female M. similis.
[Mh] Termos MeSH primário: Ascoviridae/fisiologia
Spodoptera/parasitologia
Spodoptera/virologia
Viroses/transmissão
Vespas/parasitologia
Vespas/virologia
[Mh] Termos MeSH secundário: Animais
Feminino
Interações Hospedeiro-Parasita
Insetos Vetores
Larva/parasitologia
Larva/virologia
Estágios do Ciclo de Vida
Masculino
Oviposição
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160217
[St] Status:MEDLINE
[do] DOI:10.1038/srep21296


  5 / 37 MEDLINE  
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[PMID]:25562178
[Au] Autor:Piégu B; Asgari S; Bideshi D; Federici BA; Bigot Y
[Ad] Endereço:UMR INRA-CNRS 7247, PRC, Centre INRA de Nouzilly, 37380 Nouzilly, France.
[Ti] Título:Evolutionary relationships of iridoviruses and divergence of ascoviruses from invertebrate iridoviruses in the superfamily Megavirales.
[So] Source:Mol Phylogenet Evol;84:44-52, 2015 Mar.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The family Iridoviridae of the superfamily Megavirales currently consists of five genera. Three of these, Lymphocystivirus, Megalocytivirus and Ranavirus, are composed of species that infect vertebrates, and the other two, Chloriridovirus and Iridovirus, contain species that infect invertebrates. Until recently, the lack of genomic sequence data limited investigation of the evolutionary relationships between the invertebrate iridoviruses (IIVs) and vertebrate iridoviruses (VIVs), as well as the relationship of these viruses to those of the closely related family Ascoviridae, which only contains species that infect insects. To help clarify the phylogenetic relationships of these viruses, we recently published the annotated genome sequences of five additional IIV isolates. Here, using classical approaches of phylogeny via maximum likelihood, a Bayesian approach, and resolution of a core protein tree, we demonstrate that the invertebrate and vertebrate IV species constitute two lineages that diverged early during the evolution of the family Iridoviridae, before the emergence of the four IIV clades, previously referred to as Chloriridoviruses, Polyiridoviruses, Oligoiridoviruses and Crustaceoiridoviruses. In addition, we provide evidence that species of the family Ascoviridae have a more recent origin than most iridoviruses, emerging just before the differentiation between the Oligoiridoviruses and Crustaceoiridovirus clades. Our results also suggest that after emergence, based on their molecular clock, the ascoviruses evolved more quickly than their closest iridovirus relatives.
[Mh] Termos MeSH primário: Ascoviridae/classificação
Evolução Biológica
Iridoviridae/classificação
Filogenia
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
Genoma Viral
Insetos/virologia
Invertebrados/virologia
Funções Verossimilhança
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1506
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150107
[St] Status:MEDLINE


  6 / 37 MEDLINE  
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[PMID]:25149038
[Au] Autor:Wei YL; Hu J; Li SJ; Chen ZS; Cheng XW; Huang GH
[Ad] Endereço:Institute of Virology, Hunan Agricultural University, Changsha, Hunan 410128, China; Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, China.
[Ti] Título:Genome sequence and organization analysis of Heliothis virescens ascovirus 3f isolated from a Helicoverpa zea larva.
[So] Source:J Invertebr Pathol;122:40-3, 2014 Oct.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The complete genome sequence of Heliothis virescens ascovirus 3f (HvAV-3f) was obtained. The HvAV-3f genome has a circular genome of 198,157bp with a G+C content of 46.0%, and encodes 190 open reading frames (ORFs) longer than 69 amino acids. Two major homologous regions (hrs) and 29 'baculovirus repeat ORFs' (bro) were found in the genome. BLAST analyses revealed that three HvAV-3f genes were homologous to that of lepidopteran insects. Nine ORFs were unique to HvAV-3f, in which two ORFs showed significant levels of similarity to genes that have not been previously described for ascoviruses in the Genbank database.
[Mh] Termos MeSH primário: Ascoviridae/genética
DNA Viral/genética
Genoma Viral/genética
Zea mays/virologia
[Mh] Termos MeSH secundário: Animais
DNA Viral/análise
Larva/virologia
Análise de Sequência de DNA
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:141007
[Lr] Data última revisão:
141007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140824
[St] Status:MEDLINE


  7 / 37 MEDLINE  
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[PMID]:24386488
[Au] Autor:Li SJ; Wang X; Zhou ZS; Zhu J; Hu J; Zhao YP; Zhou GW; Huang GH
[Ad] Endereço:Institute of Virology, Hunan Agricultural University, Changsha, Hunan, China.
[Ti] Título:A comparison of growth and development of three major agricultural insect pests infected with Heliothis virescens ascovirus 3h (HvAV-3h).
[So] Source:PLoS One;8(12):e85704, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ascoviruses are double-stranded DNA viruses that are pathogenic to lepidopteran hosts, particularly noctuid larvae. Infection of a larva is characterized by retarded growth, reduced feeding and yellowish body color. In this paper, we reported the growth and development of three major agricultural noctuid insect pests, Helicoverpa armigera (Hübner), Spodoptera exigua (Hübner) and Spodoptera litura (Fabricius), infected with Heliothis virescens ascovirus 3h (HvAV-3h). Using 10-fold serial dilutions (0 to 7) of HvAV-3h-containing hemolymph to infect S. litura larvae, we found no significant difference in larval mortalities from 0 to 10(3)-fold dilutions; however, significant differences were observed at 10(4)-fold dilution and above. Using a 10-fold dilution of HvAV-3h-containing hemolymph to infect H. armigera, S. exigua and S. litura larvae, we found that the growth and development were significantly affected. All infected larvae could not pupate; the survival times of treated H. armigera, S. litura and S. exigua larvae were significantly longer than untreated control larvae. Body weight showed significant difference between treated and untreated control group from day 1 after inoculation in H. armigera and S. exigua, but day 2 in S. litura. Additionally, food intake also showed significant difference between treated and untreated control group from day 2 after inoculation in H. armigera and S. litura, but day 3 in S. exigua.
[Mh] Termos MeSH primário: Ascoviridae/metabolismo
Lepidópteros/virologia
[Mh] Termos MeSH secundário: Animais
Ascoviridae/patogenicidade
Hemolinfa/virologia
Larva/genética
Larva/metabolismo
Lepidópteros/metabolismo
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1502
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0085704


  8 / 37 MEDLINE  
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[PMID]:23087124
[Au] Autor:Huang GH; Wang YS; Wang X; Garretson TA; Dai LY; Zhang CX; Cheng XW
[Ad] Endereço:Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, People's Republic of China.
[Ti] Título:Genomic sequence of Heliothis virescens ascovirus 3g isolated from Spodoptera exigua.
[So] Source:J Virol;86(22):12467-8, 2012 Nov.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Heliothis virescens ascovirus 3a (HvAV-3a), a member of the family Ascoviridae, has the highest diversity among ascovirus species that have been reported in Australia, Indonesia, China, and the United States. To understand the diversity and origin of this important ascovirus, the complete genome of the HvAV Indonesia strain (HvAV-3g), isolated from Spodoptera exigua, was determined to be 199,721 bp, with a G+C content of 45.9%. Therefore, HvAV-3g has the largest genome among the reported ascovirus genomes to date. There are 194 predicted open reading frames (ORFs) encoding proteins of 50 or more amino acid residues. In comparison to HvAV-3e reported from Australia, HvAV-3g has all the ORFs in HvAV-3e with 6 additional ORFs unique to HvAV-3g, including 1 peptidase C26 gene with the highest identity to Drosophila spp. and 2 gas vesicle protein U (GvpU) genes with identities to Bacillus megaterium. The five unique homologous regions (hrs) and 25 baculovirus repeat ORFs (bro) of HvAV-3g are highly variable.
[Mh] Termos MeSH primário: Ascoviridae/genética
Genoma Viral
Spodoptera/virologia
[Mh] Termos MeSH secundário: Animais
DNA Viral
Bases de Dados Genéticas
Genes Virais
Variação Genética
Dados de Sequência Molecular
Fases de Leitura Aberta
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1301
[Cu] Atualização por classe:150222
[Lr] Data última revisão:
150222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121023
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.02342-12


  9 / 37 MEDLINE  
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[PMID]:22792245
[Au] Autor:Huang GH; Garretson TA; Cheng XH; Holztrager MS; Li SJ; Wang X; Cheng XW
[Ad] Endereço:Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan, China.
[Ti] Título:Phylogenetic position and replication kinetics of Heliothis virescens ascovirus 3h (HvAV-3h) isolated from Spodoptera exigua.
[So] Source:PLoS One;7(7):e40225, 2012.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Insect-specific ascoviruses with a circular genome are distributed in the USA, France, Australia and Indonesia. Here, we report the first ascovirus isolation from Spodoptera exigua in Hunan, China. DNA-DNA hybridization to published ascoviruses demonstrated that the new China ascovirus isolate is a variant of Heliothis virescens ascovirus 3a (HvAV-3a), thus named HvAV-3h. We investigated the phylogenetic position, cell infection, vesicle production and viral DNA replication kinetics of HvAV-3h, as well as its host-ranges. The major capsid protein (MCP) gene and the delta DNA polymerase (DNA po1) gene of HvAV-3h were sequenced and compared with the available ascovirus isolates for phylogenetic analysis. This shows a close relationship with HvAV-3g, originally isolated from Indonesia, HvAV-3e from Australia and HvAV-3c from United States. HvAV-3h infection induced vesicle production in the SeE1 cells derived from S. exigua and Sf9 cells derived from S. frugiperda, resulting in more vesicles generated in Sf9 than SeE1. Viral DNA replication kinetics of HvAV-3h also demonstrated a difference between the two cell lines tested. HvAV-3h could readily infect three important insect pests Helicoverpa armigera (Hübner), Spodoptera exigua (Hübner) and Spodoptera litura (Fabricius) from two genera in different subfamilies with high mortalities.
[Mh] Termos MeSH primário: Ascoviridae/genética
Filogenia
Spodoptera/virologia
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Ascoviridae/classificação
Ascoviridae/isolamento & purificação
Ascoviridae/ultraestrutura
DNA Viral/genética
Cinética
Células Sf9
Proteínas Virais/genética
Tropismo Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1303
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120714
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0040225


  10 / 37 MEDLINE  
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[PMID]:21832004
[Au] Autor:Xue JL; Cheng XW
[Ad] Endereço:Department of Microbiology, 32 Pearson Hall, Miami University, Oxford, OH 45056, USA.
[Ti] Título:Comparative analysis of a highly variable region within the genomes of Spodoptera frugiperda ascovirus 1d (SfAV-1d) and SfAV-1a.
[So] Source:J Gen Virol;92(Pt 12):2797-802, 2011 Dec.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The recently discovered ascoviruses have a worldwide distribution. Here we report a new member of the family Ascoviridae, Spodoptera frugiperda ascovirus 1d (SfAV-1d) with a variable region in the genome. Restriction fragment length polymorphism, Southern hybridization and genome sequencing analyses confirmed that SfAV-1d and the earlier reported SfAV-1a are closely related but are not identical. The genome size of SfAV-1d is approximately 100 kbp, which is about 57 kbp smaller than SfAV-1a. The SfAV-1d genome has a major deletion of 14 kbp that corresponds to one of the inverted repeat (IR) regions of SfAV-1a. Cloning and sequencing revealed that the region flanking the deletion within the SfAV-1d genome is highly variable. In all the variants of this region, the whole IR region is missing, with 88.2 % of the variants missing part of or the whole adjacent SfAV-1a ORF71, 94.1 % missing part of or the whole of adjacent ORF72 and 64.6 % missing part of or the whole of ORF73.
[Mh] Termos MeSH primário: Ascoviridae/genética
DNA Viral/genética
Genoma Viral
Spodoptera/virologia
[Mh] Termos MeSH secundário: Animais
Ascoviridae/classificação
Ascoviridae/isolamento & purificação
Clonagem Molecular
Deleção de Genes
Tamanho do Genoma
Dados de Sequência Molecular
Fases de Leitura Aberta
Polimorfismo de Fragmento de Restrição
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1201
[Cu] Atualização por classe:111117
[Lr] Data última revisão:
111117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110812
[St] Status:MEDLINE
[do] DOI:10.1099/vir.0.035733-0



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde