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  1 / 19 MEDLINE  
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[PMID]:28218698
[Au] Autor:Mulumba-Mfumu LK; Achenbach JE; Mauldin MR; Dixon LK; Tshilenge CG; Thiry E; Moreno N; Blanco E; Saegerman C; Lamien CE; Diallo A
[Ad] Endereço:Central Veterinary Laboratory, Avenue Wangata, P.O. Box 8842, Kinshasa I, Democratic Republic of Congo. labovetkin@yahoo.fr.
[Ti] Título:Genetic Assessment of African Swine Fever Isolates Involved in Outbreaks in the Democratic Republic of Congo between 2005 and 2012 Reveals Co-Circulation of p72 Genotypes I, IX and XIV, Including 19 Variants.
[So] Source:Viruses;9(2), 2017 Feb 18.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated-except for Sardinia-followed by re­introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005-2012, were examined for viral DNA and sequencing at multiple loci: C-terminus of the gene (p72 protein), central hypervariable region (CVR) of the gene, and the gene (p54 protein). Phylogenetic analyses identified three circulating genotypes: I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants.
[Mh] Termos MeSH primário: Febre Suína Africana/epidemiologia
Febre Suína Africana/virologia
Asfarviridae/classificação
Asfarviridae/isolamento & purificação
Surtos de Doenças
Genótipo
[Mh] Termos MeSH secundário: Animais
Asfarviridae/genética
Análise por Conglomerados
DNA Viral/química
DNA Viral/genética
República Democrática do Congo/epidemiologia
Epidemiologia Molecular
Filogenia
Análise de Sequência de DNA
Sus scrofa
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


  2 / 19 MEDLINE  
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[PMID]:27185929
[Au] Autor:Klose T; Reteno DG; Benamar S; Hollerbach A; Colson P; La Scola B; Rossmann MG
[Ad] Endereço:Department of Biological Sciences, Purdue University, West Lafayette, IN 47907;
[Ti] Título:Structure of faustovirus, a large dsDNA virus.
[So] Source:Proc Natl Acad Sci U S A;113(22):6206-11, 2016 May 31.
[Is] ISSN:1091-6490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many viruses protect their genome with a combination of a protein shell with or without a membrane layer. Here we describe the structure of faustovirus, the first DNA virus (to our knowledge) that has been found to use two protein shells to encapsidate and protect its genome. The crystal structure of the major capsid protein, in combination with cryo-electron microscopy structures of two different maturation stages of the virus, shows that the outer virus shell is composed of a double jelly-roll protein that can be found in many double-stranded DNA viruses. The structure of the repeating hexameric unit of the inner shell is different from all other known capsid proteins. In addition to the unique architecture, the region of the genome that encodes the major capsid protein stretches over 17,000 bp and contains a large number of introns and exons. This complexity might help the virus to rapidly adapt to new environments or hosts.
[Mh] Termos MeSH primário: Asfarviridae/química
Proteínas do Capsídeo/química
Proteínas do Capsídeo/genética
DNA Viral/química
Genoma Viral
Vírion/química
[Mh] Termos MeSH secundário: Asfarviridae/genética
Asfarviridae/ultraestrutura
Proteínas do Capsídeo/metabolismo
Microscopia Crioeletrônica
Cristalografia por Raios X
Modelos Moleculares
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (DNA, Viral)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160518
[St] Status:MEDLINE
[do] DOI:10.1073/pnas.1523999113


  3 / 19 MEDLINE  
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[PMID]:25878099
[Au] Autor:Reteno DG; Benamar S; Khalil JB; Andreani J; Armstrong N; Klose T; Rossmann M; Colson P; Raoult D; La Scola B
[Ti] Título:Faustovirus, an asfarvirus-related new lineage of giant viruses infecting amoebae.
[So] Source:J Virol;89(13):6585-94, 2015 Jul.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Giant viruses are protist-associated viruses belonging to the proposed order Megavirales; almost all have been isolated from Acanthamoeba spp. Their isolation in humans suggests that they are part of the human virome. Using a high-throughput strategy to isolate new giant viruses from their original protozoan hosts, we obtained eight isolates of a new giant viral lineage from Vermamoeba vermiformis, the most common free-living protist found in human environments. This new lineage was proposed to be the faustovirus lineage. The prototype member, faustovirus E12, forms icosahedral virions of ≈ 200 nm that are devoid of fibrils and that encapsidate a 466-kbp genome encoding 451 predicted proteins. Of these, 164 are found in the virion. Phylogenetic analysis of the core viral genes showed that faustovirus is distantly related to the mammalian pathogen African swine fever virus, but it encodes ≈ 3 times more mosaic gene complements. About two-thirds of these genes do not show significant similarity to genes encoding any known proteins. These findings show that expanding the panel of protists to discover new giant viruses is a fruitful strategy. IMPORTANCE: By using Vermamoeba, a protist living in humans and their environment, we isolated eight strains of a new giant virus that we named faustovirus. The genomes of these strains were sequenced, and their sequences showed that faustoviruses are related to but different from the vertebrate pathogen African swine fever virus (ASFV), which belongs to the family Asfarviridae. Moreover, the faustovirus gene repertoire is ≈ 3 times larger than that of ASFV and comprises approximately two-thirds ORFans (open reading frames [ORFs] with no detectable homology to other ORFs in a database).
[Mh] Termos MeSH primário: Asfarviridae/isolamento & purificação
Asfarviridae/fisiologia
Lobosea/virologia
[Mh] Termos MeSH secundário: Asfarviridae/ultraestrutura
Análise por Conglomerados
DNA Viral/química
DNA Viral/genética
Genoma Viral
Microscopia Eletrônica de Transmissão
Dados de Sequência Molecular
Fases de Leitura Aberta
Filogenia
Proteoma/análise
Análise de Sequência de DNA
Homologia de Sequência
Proteínas Virais/genética
Vírion/química
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Proteome); 0 (Viral Proteins)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150417
[St] Status:MEDLINE


  4 / 19 MEDLINE  
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[PMID]:25776540
[Au] Autor:Grau FR; Schroeder ME; Mulhern EL; McIntosh MT; Bounpheng MA
[Ad] Endereço:Texas A&M Veterinary Medical Diagnostic Laboratory, College Station, TX (Schroeder, Bounpheng)Foreign Animal Disease Diagnostic Laboratory, U.S. Department of Agriculture, Animal and Plant Health Inspection Services, Veterinary Services, National Veterinary Services Laboratories, Plum Island Ani
[Ti] Título:Detection of African swine fever, classical swine fever, and foot-and-mouth disease viruses in swine oral fluids by multiplex reverse transcription real-time polymerase chain reaction.
[So] Source:J Vet Diagn Invest;27(2):140-9, 2015 Mar.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:African swine fever (ASF), classical swine fever (CSF), and foot-and-mouth disease (FMD) are highly contagious animal diseases of significant economic importance. Pigs infected with ASF and CSF viruses (ASFV and CSFV) develop clinical signs that may be indistinguishable from other diseases. Likewise, various causes of vesicular disease can mimic clinical signs caused by the FMD virus (FMDV). Early detection is critical to limiting the impact and spread of these disease outbreaks, and the ability to perform herd-level surveillance for all 3 diseases rapidly and cost effectively using a single diagnostic sample and test is highly desirable. This study assessed the feasibility of simultaneous ASFV, CSFV, and FMDV detection by multiplex reverse transcription real-time polymerase chain reaction (mRT-qPCR) in swine oral fluids collected through the use of chewing ropes. Animal groups were experimentally infected independently with each virus, observed for clinical signs, and oral fluids collected and tested throughout the course of infection. All animal groups chewed on the ropes readily before and after onset of clinical signs and before onset of lameness or serious clinical signs. ASFV was detected as early as 3 days postinoculation (dpi), 2-3 days before onset of clinical disease; CSFV was detected at 5 dpi, coincident with onset of clinical disease; and FMDV was detected as early as 1 dpi, 1 day before the onset of clinical disease. Equivalent results were observed in 4 independent studies and demonstrate the feasibility of oral fluids and mRT-qPCR for surveillance of ASF, CSF, and FMD in swine populations.
[Mh] Termos MeSH primário: Asfarviridae/isolamento & purificação
Vírus da Febre Suína Clássica/isolamento & purificação
Vírus da Febre Aftosa/isolamento & purificação
Doenças dos Suínos/diagnóstico
[Mh] Termos MeSH secundário: Febre Suína Africana/diagnóstico
Febre Suína Africana/virologia
Animais
Asfarviridae/genética
Peste Suína Clássica/diagnóstico
Peste Suína Clássica/virologia
Vírus da Febre Suína Clássica/genética
DNA Viral/análise
Febre Aftosa/diagnóstico
Febre Aftosa/virologia
Vírus da Febre Aftosa/genética
Boca/virologia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Transcrição Reversa
Sensibilidade e Especificidade
Suínos
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150317
[Lr] Data última revisão:
150317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150318
[St] Status:MEDLINE
[do] DOI:10.1177/1040638715574768


  5 / 19 MEDLINE  
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[PMID]:24369729
[Au] Autor:Atuhaire DK; Afayoa M; Ochwo S; Mwesigwa S; Mwiine FN; Okuni JB; Olaho-Mukani W; Ojok L
[Ti] Título:Prevalence of African swine fever virus in apparently healthy domestic pigs in Uganda.
[So] Source:BMC Vet Res;9:263, 2013 Dec 26.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: African swine fever (ASF) is a contagious viral disease which can cause up to 100% mortality among domestic pigs leading to serious socio-economic impact on people's livelihoods. ASF is endemic in Uganda and there is paucity of information on the epidemiology of the disease. The major aim of this study was to determine the seroprevalence and prevalence of African swine fever virus (ASFV) in apparently healthy slaughter pigs at Wambizi slaughterhouse in Kampala city, Uganda. We also estimated the presence of ASFV antibodies and circulating viral antigens in pigs from selected districts of Uganda during targeted surveillance. We analysed 540 and 181 blood samples collected from slaughter pigs and pigs from targeted surveillance districts respectively. RESULTS: The prevalence of ASFV in slaughter pigs was 52.96% (95% CI, 48.75-57.14) and 11.5% (95% CI, 9.06-14.45) by ELISA and PCR respectively. In surveillance districts, the proportion of ASFV positive pigs was 53.59% (95% CI, 46.33-60.71) and 0.55% (95% CI, 0.1-3.06) by ELISA and PCR respectively. CONCLUSION: The study has found out a high seroprevalence of ASFV antibodies in apparently healthy slaughter pigs and also a high proportion of ASFV antibody seropositive pigs in surveyed districts in Uganda indicating exposure to ASFV. However, there was a lower prevalence of ASFV infection implying that there could be low virulent strains of ASFV circulating in domestic pigs in Uganda which requires further investigation.
[Mh] Termos MeSH primário: Febre Suína Africana/epidemiologia
Asfarviridae
[Mh] Termos MeSH secundário: Matadouros
Animais
Anticorpos Antivirais/sangue
Infecções Assintomáticas/epidemiologia
Estudos Transversais
Ensaio de Imunoadsorção Enzimática/veterinária
Reação em Cadeia da Polimerase/veterinária
Prevalência
Suínos/virologia
Uganda/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1408
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131228
[St] Status:MEDLINE
[do] DOI:10.1186/1746-6148-9-263


  6 / 19 MEDLINE  
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[PMID]:23923045
[Au] Autor:Haines FJ; Hofmann MA; King DP; Drew TW; Crooke HR
[Ad] Endereço:Virology Department, Animal Health and Veterinary Laboratories Agency, New Haw, Surrey, United Kingdom.
[Ti] Título:Development and validation of a multiplex, real-time RT PCR assay for the simultaneous detection of classical and African swine fever viruses.
[So] Source:PLoS One;8(7):e71019, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A single-step, multiplex, real-time polymerase chain reaction (RT-PCR) was developed for the simultaneous and differential laboratory diagnosis of Classical swine fever virus (CSFV) and African swine fever virus (ASFV) alongside an exogenous internal control RNA (IC-RNA). Combining a single extraction methodology and primer and probe sets for detection of the three target nucleic acids CSFV, ASFV and IC-RNA, had no effect on the analytical sensitivity of the assay and the new triplex RT-PCR was comparable to standard PCR techniques for CSFV and ASFV diagnosis. After optimisation the assay had a detection limit of 5 CSFV genome copies and 22 ASFV genome copies. Analytical specificity of the triplex assay was validated using a panel of viruses representing 9 of the 11 CSFV subgenotypes, at least 8 of the 22 ASFV genotypes as well as non-CSFV pestiviruses. Positive and negative clinical samples from animals infected experimentally, due to field exposure or collected from the UK which is free from both swine diseases, were used to evaluate the diagnostic sensitivity and specificity for detection of both viruses. The diagnostic sensitivity was 100% for both viruses whilst diagnostic specificity estimates were 100% for CSFV detection and 97.3% for ASFV detection. The inclusion of a heterologous internal control allowed identification of false negative results, which occurred at a higher level than expected. The triplex assay described here offers a valuable new tool for the differential detection of the causative viruses of two clinically indistinguishable porcine diseases, whose geographical occurrence is increasingly overlapping.
[Mh] Termos MeSH primário: Vírus da Febre Suína Africana/genética
Febre Suína Africana/diagnóstico
Asfarviridae/genética
Infecções por Vírus de DNA/diagnóstico
Reação em Cadeia da Polimerase em Tempo Real/métodos
Doenças dos Suínos/diagnóstico
Suínos/virologia
[Mh] Termos MeSH secundário: Febre Suína Africana/virologia
Vírus da Febre Suína Africana/isolamento & purificação
Animais
Asfarviridae/isolamento & purificação
Infecções por Vírus de DNA/veterinária
Infecções por Vírus de DNA/virologia
Sensibilidade e Especificidade
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1404
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130808
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0071019


  7 / 19 MEDLINE  
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[PMID]:23338931
[Au] Autor:Wan XF; Barnett JL; Cunningham F; Chen S; Yang G; Nash S; Long LP; Ford L; Blackmon S; Zhang Y; Hanson L; He Q
[Ad] Endereço:Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi state, MS, USA. wanhenry@yahoo.com
[Ti] Título:Detection of African swine fever virus-like sequences in ponds in the Mississippi Delta through metagenomic sequencing.
[So] Source:Virus Genes;46(3):441-6, 2013 Jun.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Metagenomic characterization of water virome was performed in four Mississippi catfish ponds. Although differing considerably from African swine fever virus (ASFV), 48 of 446,100 sequences from 12 samples were similar enough to indicate that they represent new members in the family Asfarviridae. At present, ASFV is the only member of Asfarviridae, and this study presents the first indication of a similar virus in North America. At this point, there is no indication that the identified virus(es) pose a threat to human or animal health, and further study is needed to characterize their potential risks to both public health and agricultural development.
[Mh] Termos MeSH primário: Asfarviridae/classificação
Asfarviridae/genética
Metagenômica
Tanques/virologia
Rios/virologia
[Mh] Termos MeSH secundário: Animais
Aquicultura
Asfarviridae/isolamento & purificação
Peixes-Gato
América do Norte
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1312
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130123
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-013-0878-2


  8 / 19 MEDLINE  
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[PMID]:23012981
[Au] Autor:Karalova EM; Arzumanian GA; Zakarian OS; Voskanian GE; Sarkisian KhV; Karalian ZA
[Ti] Título:[Dynamics of changes in the composition of leukocyte population of peripheral blood during the African swine fever].
[So] Source:Vopr Virusol;57(4):27-31, 2012 Jul-Aug.
[Is] ISSN:0507-4088
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The comparison of the composition of leukocytes of peripheral blood of healthy and Infected swine revealed the quantitative alterations, as well as the appearance of new cells in leukocyte population during African swine fever. It was determined that African swine fever virus induced mass-scale mortality of cells of peripheral blood, especially lymphocytes and neutrophiles. The number of the dead cells reaches 60% of the initial number of all cells at the end of infection. It was also revealed that the appearance of atypical lymphocytes and lymphoblasts was observed during viral Infections. Most of these cells are characterized by the presence of additional nucleus.
[Mh] Termos MeSH primário: Febre Suína Africana
Suínos
[Mh] Termos MeSH secundário: Febre Suína Africana/sangue
Febre Suína Africana/virologia
Animais
Asfarviridae/patogenicidade
Contagem de Células Sanguíneas
Eritroblastos/patologia
Linfócitos/patologia
Neutrófilos/patologia
Suínos/sangue
Suínos/virologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Mês de entrada:1211
[Cu] Atualização por classe:161109
[Lr] Data última revisão:
161109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120928
[St] Status:MEDLINE


  9 / 19 MEDLINE  
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[PMID]:22932353
[Au] Autor:Callaway E
[Ti] Título:Pig fever sweeps across Russia.
[So] Source:Nature;488(7413):565-6, 2012 Aug 30.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Febre Suína Africana/epidemiologia
Febre Suína Africana/transmissão
Surtos de Doenças/veterinária
Sus scrofa/virologia
[Mh] Termos MeSH secundário: Febre Suína Africana/economia
Febre Suína Africana/prevenção & controle
Ração Animal
Animais
Asfarviridae/genética
Asfarviridae/imunologia
Surtos de Doenças/economia
Indústria Alimentícia/economia
Federação Russa/epidemiologia
Suínos/virologia
Incerteza
Vacinas Virais/imunologia
[Pt] Tipo de publicação:NEWS
[Nm] Nome de substância:
0 (Viral Vaccines)
[Em] Mês de entrada:1210
[Cu] Atualização por classe:120830
[Lr] Data última revisão:
120830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120831
[St] Status:MEDLINE
[do] DOI:10.1038/488565a


  10 / 19 MEDLINE  
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[PMID]:21554353
[Au] Autor:Palgrave CJ; Lu ZH; Lowden CS; Whitelaw CB
[Ad] Endereço:Veterinary Pathology Unit, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK. Chris.Palgrave@ed.ac.uk
[Ti] Título:Dynein light chain 1 (LC8) sequence is highly conserved between pig species.
[So] Source:Anim Genet;42(3):337, 2011 Jun.
[Is] ISSN:1365-2052
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Sequência Conservada/genética
Dineínas/genética
Suínos/genética
[Mh] Termos MeSH secundário: Febre Suína Africana/genética
Sequência de Aminoácidos
Animais
Asfarviridae
Sequência de Bases
Dados de Sequência Molecular
Reação em Cadeia da Polimerase
Polimorfismo de Nucleotídeo Único
Análise de Sequência de DNA
Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.6.4.2 (Dyneins)
[Em] Mês de entrada:1110
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110511
[St] Status:MEDLINE
[do] DOI:10.1111/j.1365-2052.2010.02143.x



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