Base de dados : MEDLINE
Pesquisa : B04.280.382.100 [Categoria DeCS]
Referências encontradas : 312 [refinar]
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[PMID]:28406478
[Au] Autor:You Y; Cheng AC; Wang MS; Jia RY; Sun KF; Yang Q; Wu Y; Zhu D; Chen S; Liu MF; Zhao XX; Chen XY
[Ad] Endereço:Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City 611130, Sichuan, P.R. China.
[Ti] Título:The suppression of apoptosis by α-herpesvirus.
[So] Source:Cell Death Dis;8(4):e2749, 2017 Apr 13.
[Is] ISSN:2041-4889
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Apoptosis, an important innate immune mechanism that eliminates pathogen-infected cells, is primarily triggered by two signalling pathways: the death receptor pathway and the mitochondria-mediated pathway. However, many viruses have evolved various strategies to suppress apoptosis by encoding anti-apoptotic factors or regulating apoptotic signalling pathways, which promote viral propagation and evasion of the host defence. During its life cycle, α-herpesvirus utilizes an elegant multifarious anti-apoptotic strategy to suppress programmed cell death. This progress article primarily focuses on the current understanding of the apoptosis-inhibition mechanisms of α-herpesvirus anti-apoptotic genes and their expression products and discusses future directions, including how the anti-apoptotic function of herpesvirus could be targeted therapeutically.
[Mh] Termos MeSH primário: Alphaherpesvirinae/fisiologia
Proteínas Reguladoras de Apoptose/metabolismo
Apoptose
Infecções por Herpesviridae/metabolismo
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Infecções por Herpesviridae/patologia
Infecções por Herpesviridae/terapia
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins); 0 (Viral Proteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1038/cddis.2017.139


  2 / 312 MEDLINE  
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[PMID]:28160144
[Au] Autor:Eberle R; Black DH
[Ad] Endereço:Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, 74078, USA. r.eberle@okstate.edu.
[Ti] Título:Sequence of the ateline alphaherpesvirus 1 (HVA1) genome.
[So] Source:Arch Virol;162(5):1423-1425, 2017 May.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Here, we report the genome sequence of a spider monkey alphaherpesvirus (ateline alphaherpesvirus 1, HVA1) and compare it with that of other primate alphaherpesviruses. The HVA1 genome is 147,346 bp long and contains 67 predicted ORFs. The genetic layout of the HVA1 genome is similar to that of the squirrel monkey alphaherpesvirus (saimirine alphaherpesvirus 1, HVS1) in that it lacks inverted repeat regions flanking the unique long region and homologues of the UL43, UL49.5, US8.5 and US10-12 genes. Unlike HVS1, HVA1 also lacks a homologue of the RL1 (γ34.5) gene and a replication origin near the end of the genome. Consistent with previous phylogenetic analyses, all predicted proteins of HVA1 are most closely related to those of HVS1.
[Mh] Termos MeSH primário: Alphaherpesvirinae/genética
Atelinae/virologia
DNA Viral/genética
Genoma Viral/genética
Infecções por Herpesviridae/veterinária
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Alphaherpesvirinae/classificação
Alphaherpesvirinae/isolamento & purificação
Sequência de Aminoácidos
Animais
Sequência de Bases
Infecções por Herpesviridae/virologia
Origem de Replicação/genética
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3249-9


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[PMID]:28060895
[Au] Autor:Maier AK; Jung R; Villinger C; Schubert A; Walther P; Sinzger C; Lieber D
[Ad] Endereço:Institute of Virology, Ulm University Medical Center, Ulm, Germany.
[Ti] Título:A Luciferase Gene Driven by an Alphaherpesviral Promoter Also Responds to Immediate Early Antigens of the Betaherpesvirus HCMV, Allowing Comparative Analyses of Different Human Herpesviruses in One Reporter Cell Line.
[So] Source:PLoS One;12(1):e0169580, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Widely used methods for quantification of human cytomegalovirus (HCMV) infection in cell culture such as immunoblotting or plaque reduction assays are generally restricted to low throughput and require time-consuming evaluation. Up to now, only few HCMV reporter cell lines have been generated to overcome these restrictions and they are afflicted with other limitations because permanently expandable cell lines are normally not fully permissive to HCMV. In this work, a previously existing epithelial cell line hosting a luciferase gene under control of a Varicella-zoster virus promoter was adopted to investigate HCMV infection. The cells were susceptible to different HCMV strains at infection efficiencies that corresponded to their respective degree of epithelial cell tropism. Expression of early and late viral antigens, formation of nuclear inclusions, release of infectious virus progeny, and focal growth indicated productive viral replication. However, viral release and spread occurred at lower levels than in primary cell lines which appears to be due to a malfunction of virion morphogenesis during the nuclear stage. Expression of the luciferase reporter gene was specifically induced in HCMV infected cultures as a function of the virus dose and dependent on viral immediate early gene expression. The level of reporter activity accurately reflected infection efficiencies as determined by viral antigen immunostaining, and hence could discriminate the cell tropism of the tested virus strains. As proof-of-principle, we demonstrate that this cell line is applicable to evaluate drug resistance of clinical HCMV isolates and the neutralization capacity of human sera, and that it allows comparative and simultaneous analysis of HCMV and human herpes simplex virus type 1. In summary, the permanent epithelial reporter cell line allows robust, rapid and objective quantitation of HCMV infection and it will be particularly useful in higher throughput analyses as well as in comparative analyses of different human herpesviruses.
[Mh] Termos MeSH primário: Alphaherpesvirinae/genética
Citomegalovirus/fisiologia
Expressão Gênica
Genes Reporter
Proteínas Imediatamente Precoces/metabolismo
Luciferases/genética
Regiões Promotoras Genéticas
[Mh] Termos MeSH secundário: Animais
Antígenos Virais/imunologia
Antígenos Virais/metabolismo
Linhagem Celular
Citomegalovirus/ultraestrutura
Genoma Viral
Seres Humanos
Proteínas Imediatamente Precoces/imunologia
Tropismo Viral
Vírion/ultraestrutura
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Viral); 0 (Immediate-Early Proteins); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169580


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[PMID]:27871228
[Au] Autor:Biswas B; Kandpal M; Jauhari UK; Vivekanandan P
[Ad] Endereço:Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, 110016, India.
[Ti] Título:Genome-wide analysis of G-quadruplexes in herpesvirus genomes.
[So] Source:BMC Genomics;17(1):949, 2016 11 21.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: G-quadruplexes are increasingly recognized as regulatory elements in human, animal, bacterial and plant genomes. The presence and function of G-quadruplexes are not well studied among herpesviruses; in particular, there are no systematic genome-wide analysis of these important secondary structures in herpesvirus genomes. RESULTS: We performed genome-wide analysis of putative quadruplex sequences (PQS) in human herpesviruses. We found unusually high PQS densities among human herpesviruses. PQS are enriched in the repeat regions and regulatory regions of human herpesviruses. Interestingly, PQS densities are higher in regulatory regions of immediate early genes compared to early and late genes in most herpesviruses. In addition, the majority of genes functionally conserved across human herpesviruses contain one or more PQS within the regulatory regions. We also describe the existence of unique intramolecular PQS repeats or repetitive G-quadruplex motifs in herpesviruses. Functional studies confirm a role for G-quadruplexes in regulating the gene expression of human herpesviruses. CONCLUSION: The pervasiveness of PQS, their enrichment and conservation at specific genomic locations suggest that these structural entities may represent a novel class of functional elements in herpesviruses. Our findings provide the necessary framework for studies on the biological role of G-quadruplexes in herpesviruses.
[Mh] Termos MeSH primário: DNA Viral/química
DNA Viral/genética
Quadruplex G
Genoma Viral
Estudo de Associação Genômica Ampla
Genômica
Herpesviridae/genética
[Mh] Termos MeSH secundário: Alphaherpesvirinae/genética
Genes Precoces
Genômica/métodos
Seres Humanos
Regiões Promotoras Genéticas
Sequências Reguladoras de Ácido Nucleico
Sequências Repetitivas de Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE


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[PMID]:27256025
[Au] Autor:Jones K; Ariel E; Burgess G; Read M
[Ad] Endereço:College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland 4811, Australia. Electronic address: karina.jones@my.jcu.edu.au.
[Ti] Título:A review of fibropapillomatosis in Green turtles (Chelonia mydas).
[So] Source:Vet J;212:48-57, 2016 Jun.
[Is] ISSN:1532-2971
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Despite being identified in 1938, many aspects of the pathogenesis and epidemiology of fibropapillomatosis (FP) in marine turtles are yet to be fully uncovered. Current knowledge suggests that FP is an emerging infectious disease, with the prevalence varying both spatially and temporally, even between localities in close proximity to each other. A high prevalence of FP in marine turtles has been correlated with residency in areas of reduced water quality, indicating that there is an environmental influence on disease presentation. Chelonid herpesvirus 5 (ChHV5) has been identified as the likely aetiological agent of FP. The current taxonomic position of ChHV5 is in the family Herpesviridae, subfamily Alphaherpesvirinae, genus Scutavirus. Molecular differentiation of strains has revealed that a viral variant is typically present at specific locations, even within sympatric species of marine turtles, indicating that the disease FP originates regionally. There is uncertainty surrounding the exact path of transmission and the conditions that facilitate lesion development, although recent research has identified atypical genes within the genome of ChHV5 that may play a role in pathogenesis. This review discusses emerging areas where researchers might focus and theories behind the emergence of FP globally since the 1980s, which appear to be a multi-factorial interplay between the virus, the host and environmental factors influencing disease expression.
[Mh] Termos MeSH primário: Alphaherpesvirinae/fisiologia
Infecções por Herpesviridae/veterinária
Tartarugas
[Mh] Termos MeSH secundário: Alphaherpesvirinae/genética
Animais
Infecções por Herpesviridae/epidemiologia
Infecções por Herpesviridae/transmissão
Infecções por Herpesviridae/virologia
Incidência
Filogenia
Prevalência
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160604
[St] Status:MEDLINE


  6 / 312 MEDLINE  
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[PMID]:27213534
[Au] Autor:Papageorgiou KV; Suárez NM; Wilkie GS; McDonald M; Graham EM; Davison AJ
[Ad] Endereço:MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.
[Ti] Título:Genome Sequence of Canine Herpesvirus.
[So] Source:PLoS One;11(5):e0156015, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Canine herpesvirus is a widespread alphaherpesvirus that causes a fatal haemorrhagic disease of neonatal puppies. We have used high-throughput methods to determine the genome sequences of three viral strains (0194, V777 and V1154) isolated in the United Kingdom between 1985 and 2000. The sequences are very closely related to each other. The canine herpesvirus genome is estimated to be 125 kbp in size and consists of a unique long sequence (97.5 kbp) and a unique short sequence (7.7 kbp) that are each flanked by terminal and internal inverted repeats (38 bp and 10.0 kbp, respectively). The overall nucleotide composition is 31.6% G+C, which is the lowest among the completely sequenced alphaherpesviruses. The genome contains 76 open reading frames predicted to encode functional proteins, all of which have counterparts in other alphaherpesviruses. The availability of the sequences will facilitate future research on the diagnosis and treatment of canine herpesvirus-associated disease.
[Mh] Termos MeSH primário: Alphaherpesvirinae/genética
Doenças do Cão/virologia
Genoma Viral
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Mapeamento Cromossômico
Cães
Genes Virais
Transtornos Hemorrágicos/veterinária
Transtornos Hemorrágicos/virologia
Células Madin Darby de Rim Canino
Fases de Leitura Aberta
Análise de Sequência de DNA
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160524
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0156015


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[PMID]:27041357
[Au] Autor:Ishihara Y; Esaki M; Yasuda A
[Ad] Endereço:Ceva Animal Health (Japan Campus), 1-6 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
[Ti] Título:Removal of Inserted BAC after linearizatiON (RIBON)-a novel strategy to excise the mini-F sequences from viral BAC vectors.
[So] Source:J Vet Med Sci;78(7):1129-36, 2016 Aug 01.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The bacterial artificial chromosome (BAC) technology has been a mainstay approach for generating recombinant viruses, and several methods for excision of the mini-F sequences from the viral BAC vectors have been developed. However, these strategies either require complicated procedures or leave scars of inserted sequences. To overcome these problems, a new method to excise the mini-F sequences from viral BAC vectors based on the Removal of Inserted BAC after linearizatiON (RIBON) strategy was developed in this study for herpesvirus of turkeys (HVT). Enhanced green fluorescent protein (eGFP) DNA and the mini-F sequences were inserted into the gene encoding HVT thymidine kinase (TK) by homologous recombination in chicken embryo fibroblasts (CEFs), and the constructed HVT-BAC vector was used to transform Escherichia coli (pHVT-BAC). To remove the inserted eGFP and mini-F sequences, pHVT-BAC was linearized using a homing endonuclease I-SceI and used to cotransfect CEFs together with a plasmid containing the TK gene of HVT. The obtained viruses (44%) did not express eGFP, and DNA sequencing of isolated clones revealed that they were completely free of the inserted BAC sequences. Moreover, growth kinetics and plaque morphology of reconstituted viruses were comparable with those of the parental HVT. The results of this study demonstrate that the novel RIBON approach to remove mini-F sequences from the viral genome is simple and effective.
[Mh] Termos MeSH primário: Cromossomos Artificiais Bacterianos/genética
Vetores Genéticos
[Mh] Termos MeSH secundário: Alphaherpesvirinae/genética
Animais
Sequência de Bases
Embrião de Galinha
Replicon/genética
Deleção de Sequência
Timidina Quinase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.7.1.21 (Thymidine Kinase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170525
[Lr] Data última revisão:
170525
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160405
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.16-0038


  8 / 312 MEDLINE  
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[PMID]:26810224
[Au] Autor:Jing L; Laing KJ; Dong L; Russell RM; Barlow RS; Haas JG; Ramchandani MS; Johnston C; Buus S; Redwood AJ; White KD; Mallal SA; Phillips EJ; Posavad CM; Wald A; Koelle DM
[Ad] Endereço:Department of Medicine, University of Washington, Seattle, USA.
[Ti] Título:Extensive CD4 and CD8 T Cell Cross-Reactivity between Alphaherpesviruses.
[So] Source:J Immunol;196(5):2205-2218, 2016 Mar 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Alphaherpesvirinae subfamily includes HSV types 1 and 2 and the sequence-divergent pathogen varicella zoster virus (VZV). T cells, controlled by TCR and HLA molecules that tolerate limited epitope amino acid variation, might cross-react between these microbes. We show that memory PBMC expansion with either HSV or VZV enriches for CD4 T cell lines that recognize the other agent at the whole-virus, protein, and peptide levels, consistent with bidirectional cross-reactivity. HSV-specific CD4 T cells recovered from HSV-seronegative persons can be explained, in part, by such VZV cross-reactivity. HSV-1-reactive CD8 T cells also cross-react with VZV-infected cells, full-length VZV proteins, and VZV peptides, as well as kill VZV-infected dermal fibroblasts. Mono- and cross-reactive CD8 T cells use distinct TCRB CDR3 sequences. Cross-reactivity to VZV is reconstituted by cloning and expressing TCRA/TCRB receptors from T cells that are initially isolated using HSV reagents. Overall, we define 13 novel CD4 and CD8 HSV-VZV cross-reactive epitopes and strongly imply additional cross-reactive peptide sets. Viral proteins can harbor both CD4 and CD8 HSV/VZV cross-reactive epitopes. Quantitative estimates of HSV/VZV cross-reactivity for both CD4 and CD8 T cells vary from 10 to 50%. Based on these findings, we hypothesize that host herpesvirus immune history may influence the pathogenesis and clinical outcome of subsequent infections or vaccinations for related pathogens and that cross-reactive epitopes and TCRs may be useful for multi-alphaherpesvirus vaccine design and adoptive cellular therapy.
[Mh] Termos MeSH primário: Alphaherpesvirinae/imunologia
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD8-Positivos/imunologia
Reações Cruzadas/imunologia
Infecções por Herpesviridae/imunologia
[Mh] Termos MeSH secundário: Apresentação do Antígeno/imunologia
Antígenos Virais/imunologia
Linfócitos T CD4-Positivos/metabolismo
Linfócitos T CD8-Positivos/metabolismo
Citocinas/metabolismo
Epitopos de Linfócito T/imunologia
Infecções por Herpesviridae/genética
Infecções por Herpesviridae/virologia
Herpesvirus Humano 1/imunologia
Herpesvirus Humano 2/imunologia
Seres Humanos
Peptídeos/imunologia
Receptores de Antígenos de Linfócitos T/genética
Receptores de Antígenos de Linfócitos T/metabolismo
Subpopulações de Linfócitos T/imunologia
Subpopulações de Linfócitos T/metabolismo
Proteínas Virais/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Antigens, Viral); 0 (Cytokines); 0 (Epitopes, T-Lymphocyte); 0 (Peptides); 0 (Receptors, Antigen, T-Cell); 0 (Viral Proteins)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160127
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1502366


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[PMID]:26654843
[Au] Autor:Vissani MA; Thiry E; Dal Pozzo F; Barrandeguy M
[Ad] Endereço:Instituto de Virología, CICVyA, INTA, Las Cabañas y Los Reseros s/n, Castelar 1712, Argentina. Electronic address: vissani.aldana@inta.gob.ar.
[Ti] Título:Antiviral agents against equid alphaherpesviruses: Current status and perspectives.
[So] Source:Vet J;207:38-44, 2016 Jan.
[Is] ISSN:1532-2971
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Equid herpesvirus infections cause respiratory, neurological and reproductive syndromes. Despite preventive and control measures and the availability of vaccines and immunostimulants, herpesvirus infections still constitute a major threat to equine health and for the equine industry worldwide. Antiviral drugs, particularly nucleoside analogues and foscarnet, are successfully used for the treatment of human alphaherpesvirus infections. In equine medicine, the use of antiviral medications in alphaherpesvirus infections would decrease the excretion of virus and diminish the risk of contagion and the convalescent time in affected horses, and would also improve the clinical outcome of equine herpesvirus myeloencephalopathy. The combined use of antiviral compounds, along with vaccines, immune modulators, and effective preventive and control measures, might be beneficial in diminishing the negative impact of alphaherpesvirus infections in horses. The purpose of this review is to analyse the available information regarding the use of antiviral agents against alphaherpesviruses, with particular emphasis on equine alphaherpesvirus infections.
[Mh] Termos MeSH primário: Alphaherpesvirinae
Antivirais/uso terapêutico
Infecções por Herpesviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Infecções por Herpesviridae/tratamento farmacológico
Herpesvirus Equídeo 1
Doenças dos Cavalos/terapia
Doenças dos Cavalos/virologia
Cavalos
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antiviral Agents)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE


  10 / 312 MEDLINE  
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[PMID]:26498797
[Au] Autor:Gotesman M; Menanteau-Ledouble S; El-Matbouli M
[Ad] Endereço:Department of Biology, Technion - Israel Institute of Technology, Technion, Haifa, Israel.
[Ti] Título:Proteomic Analysis of Cytoskeleton Proteins in Fish.
[So] Source:Methods Mol Biol;1365:357-72, 2016.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this chapter, we describe laboratory protocols for rearing fish and a simple and efficient method of extracting and identifying pathogen and host proteins that may be involved in entry and replication of commercially important fish viruses. We have used the common carp (Cyprinus carpio L.) and goldfish (Cyprinus auratus) as a model system for studies of proteins involved in viral entry and replication. The chapter describes detailed protocols for maintenance of carp, cell culture, antibody purification of proteins, and use of electrospray-ionization mass spectrometry analysis to screen and identify cytoskeleton and other proteins that may be involved in viral infection and propagation in fish.
[Mh] Termos MeSH primário: Carpas/metabolismo
Proteínas do Citoesqueleto/metabolismo
Proteínas de Peixes/metabolismo
Carpa Dourada/metabolismo
Proteômica/métodos
[Mh] Termos MeSH secundário: Alphaherpesvirinae/fisiologia
Animais
Anticorpos Monoclonais/imunologia
Carpas/virologia
Células Cultivadas
Proteínas do Citoesqueleto/imunologia
Proteínas de Peixes/imunologia
Carpa Dourada/virologia
Injeções
Mononegavirais/fisiologia
Internalização do Vírus
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Cytoskeletal Proteins); 0 (Fish Proteins)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151027
[Lr] Data última revisão:
151027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151027
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-3124-8_21



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde