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  1 / 1064 MEDLINE  
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[PMID]:28665725
[Au] Autor:Kennedy DA; Cairns C; Jones MJ; Bell AS; Salathé RM; Baigent SJ; Nair VK; Dunn PA; Read AF
[Ad] Endereço:A Center for Infectious Disease Dynamics, Departments of Biology and Entomology, The Pennsylvania State University, University Park, PA 16802.
[Ti] Título:Industry-Wide Surveillance of Marek's Disease Virus on Commercial Poultry Farms.
[So] Source:Avian Dis;61(2):153-164, 2017 Jun.
[Is] ISSN:1938-4351
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Marek's disease virus is a herpesvirus of chickens that costs the worldwide poultry industry more than US$1 billion annually. Two generations of Marek's disease vaccines have shown reduced efficacy over the last half century due to evolution of the virus. Understanding where the virus is present may give insight into whether continued reductions in efficacy are likely. We conducted a 3-yr surveillance study to assess the prevalence of Marek's disease virus on commercial poultry farms, determine the effect of various factors on virus prevalence, and document virus dynamics in broiler chicken houses over short (weeks) and long (years) timescales. We extracted DNA from dust samples collected from commercial chicken and egg production facilities in Pennsylvania, USA. Quantitative PCR was used to assess wild-type virus detectability and concentration. Using data from 1018 dust samples with Bayesian generalized linear mixed effects models, we determined the factors that correlated with virus prevalence across farms. Maximum likelihood and autocorrelation function estimation on 3727 additional dust samples were used to document and characterize virus concentrations within houses over time. Overall, wild-type virus was detectable at least once on 36 of 104 farms at rates that varied substantially between farms. Virus was detected in one of three broiler-breeder operations (companies), four of five broiler operations, and three of five egg layer operations. Marek's disease virus detectability differed by production type, bird age, day of the year, operation (company), farm, house, flock, and sample. Operation (company) was the most important factor, accounting for between 12% and 63.4% of the variation in virus detectability. Within individual houses, virus concentration often dropped below detectable levels and reemerged later. These data characterize Marek's disease virus dynamics, which are potentially important to the evolution of the virus.
[Mh] Termos MeSH primário: Herpesvirus Galináceo 2/isolamento & purificação
Doença de Marek/virologia
Doenças das Aves Domésticas/virologia
Vigilância de Evento Sentinela/veterinária
[Mh] Termos MeSH secundário: Criação de Animais Domésticos/economia
Animais
Galinhas
Fazendas
Genótipo
Herpesvirus Galináceo 2/classificação
Herpesvirus Galináceo 2/genética
Doença de Marek/economia
Doença de Marek/epidemiologia
Pennsylvania
Doenças das Aves Domésticas/economia
Doenças das Aves Domésticas/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1637/11525-110216-Reg.1


  2 / 1064 MEDLINE  
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[PMID]:28591220
[Au] Autor:Dong K; Chang S; Xie Q; Black-Pyrkosz A; Zhang H
[Ad] Endereço:USDA, Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, Michigan, United States of America.
[Ti] Título:Comparative transcriptomics of genetically divergent lines of chickens in response to Marek's disease virus challenge at cytolytic phase.
[So] Source:PLoS One;12(6):e0178923, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Marek's disease (MD), caused by Marek's disease virus (MDV), remains an economically significant threat to the poultry industry worldwide. Genetic resistance to MD is a promising alternative strategy to augment current control measures (vaccination and management). However, only a few functional genes reportedly conferring MD resistance have been identified. Here, we performed a comparative transcriptomics analysis of two highly inbred yet genetically divergent lines of chickens (line 63 and 72) that are resistant and susceptible to MD, respectively, in response to a very virulent plus strain of MDV (vv+MDV) challenge at cytolytic phase. A total of 203 DEGs in response to MDV challenge were identified in the two lines. Of these, 96 DEGs were in common for both lines, in addition to 36 and 71 DEGs that were specific for line 63 and 72, respectively. Functional enrichment analysis results showed the DEGs were significantly enriched in GO terms and pathways associated with immune response. Especially, the four DEGs, FGA, ALB, FN1, and F13A1 that reportedly facilitate virus invasion or immunosuppression, were found to be significantly up-regulated in the susceptible line 72 but down-regulated in the resistant line 63 birds. These results provide new resources for future studies to further elucidate the genetic mechanism conferring MD resistance.
[Mh] Termos MeSH primário: Galinhas/genética
Galinhas/virologia
Perfilação da Expressão Gênica
Variação Genética
Herpesvirus Galináceo 2/fisiologia
Doença de Marek/genética
Doença de Marek/virologia
[Mh] Termos MeSH secundário: Animais
Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
Análise de Sequência de RNA
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178923


  3 / 1064 MEDLINE  
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[PMID]:28548038
[Au] Autor:Chakraborty P; Vervelde L; Dalziel RG; Wasson PS; Nair V; Dutia BM; Kaiser P
[Ad] Endereço:1​The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK †â€‹Present address: Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4225, Bangladesh.
[Ti] Título:Marek's disease virus infection of phagocytes: a de novo in vitro infection model.
[So] Source:J Gen Virol;98(5):1080-1088, 2017 May.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Marek's disease virus (MDV) is an alphaherpesvirus that induces T-cell lymphomas in chickens. Natural infections in vivo are caused by the inhalation of infected poultry house dust and it is presumed that MDV infection is initiated in the macrophages from where the infection is passed to B cells and activated T cells. Virus can be detected in B and T cells and macrophages in vivo, and both B and T cells can be infected in vitro. However, attempts to infect macrophages in vitro have not been successful. The aim of this study was to develop a model for infecting phagocytes [macrophages and dendritic cells (DCs)] with MDV in vitro and to characterize the infected cells. Chicken bone marrow cells were cultured with chicken CSF-1 or chicken IL-4 and chicken CSF-2 for 4 days to produce macrophages and DCs, respectively, and then co-cultured with FACS-sorted chicken embryo fibroblasts (CEFs) infected with recombinant MDV expressing EGFP. Infected phagocytes were identified and sorted by FACS using EGFP expression and phagocyte-specific mAbs. Detection of MDV-specific transcripts of ICP4 (immediate early), pp38 (early), gB (late) and Meq by RT-PCR provided evidence for MDV replication in the infected phagocytes. Time-lapse confocal microscopy was also used to demonstrate MDV spread in these cells. Subsequent co-culture of infected macrophages with CEFs suggests that productive virus infection may occur in these cell types. This is the first report of in vitro infection of phagocytic cells by MDV.
[Mh] Termos MeSH primário: Herpesvirus Galináceo 2/fisiologia
Fagócitos/virologia
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Galinhas
Técnicas de Cocultura
Doença de Marek/virologia
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000763


  4 / 1064 MEDLINE  
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[PMID]:28475033
[Au] Autor:Mwangi WN; Vasoya D; Kgosana LB; Watson M; Nair V
[Ad] Endereço:1​Avian Viral Diseases Programme, UK-China Centre of Excellence on Avian Disease Research, The Pirbright Institute, Pirbright, Surrey, UK.
[Ti] Título:Differentially expressed genes during spontaneous lytic switch of Marek's disease virus in lymphoblastoid cell lines determined by global gene expression profiling.
[So] Source:J Gen Virol;98(4):779-790, 2017 Apr.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Marek's disease virus (MDV), an alphaherpesvirus of poultry, causes Marek's disease and is characterized by visceral CD4+TCRαß+ T-cell lymphomas in susceptible hosts. Immortal cell lines harbouring the viral genome have been generated from ex vivo cultures of MD tumours. As readily available sources of large numbers of cells, MDV-transformed lymphoblastoid cell lines (LCLs) are extremely valuable for studies of virus-host interaction. While the viral genome in most cells is held in a latent state, minor populations of cells display spontaneous reactivation identifiable by the expression of lytic viral genes. Spontaneous reactivation in these cells presents an opportunity to investigate the biological processes involved in the virus reactivation. For detailed characterization of the molecular events associated with reactivation, we used two lymphoblastoid cell lines derived from lymphomas induced by pRB1B-UL47eGFP, a recombinant MDV engineered to express enhanced green fluorescent protein (EGFP) fused with the UL47. We used fluorescence-activated cell sorting to purify the low-frequency EGFP-positive cells with a spontaneously activating viral genome from the majority EGFP-negative cells and analysed their gene expression profiles by RNA-seq using Illumina HiSeq2500. Ingenuity pathway analysis on more than 2000 differentially expressed genes between the lytically infected (EGFP-positive) and latently infected (EGFP-negative) cell populations identified the biological pathways involved in the reactivation. Virus-reactivating cells exhibited differential expression of a significant number of viral genes, with hierarchical differences in expression levels. Downregulation of a number of host genes including those directly involved in T-cell activation, such as CD3, CD28, ICOS and phospholipase C, was also noticed in the LCL undergoing lytic switch.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica
Herpesvirus Galináceo 2/genética
Doença de Marek/virologia
Doenças das Aves Domésticas/virologia
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Galinhas
Regulação Viral da Expressão Gênica
Herpesvirus Galináceo 2/fisiologia
Linfoma/virologia
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000744


  5 / 1064 MEDLINE  
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[PMID]:28301231
[Au] Autor:Dunn JR; Reddy SM; Niikura M; Nair V; Fulton JE; Cheng HH
[Ad] Endereço:A United States Department of Agriculture, Agricultural Research Service, U.S. National Poultry Research Center, Avian Disease and Oncology Laboratory, East Lansing, MI 48823.
[Ti] Título:Evaluation and Identification of Marek's Disease Virus BAC Clones as Standardized Reagents for Research.
[So] Source:Avian Dis;61(1):107-114, 2017 Mar.
[Is] ISSN:1938-4351
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Marek's disease virus (MDV) is an alphaherpesvirus that causes Marek's disease (MD), a lymphoproliferative disease in chickens. Understanding of MDV gene function advanced significantly following the cloning of the MDV genome as either a series of overlapping cosmids or as a bacterial artificial chromosome (BAC), both of which could produce viable MDV. The objectives of this study were to compare multiple virulent MDV BAC clones using the Avian Disease and Oncology Laboratory's pathotyping assay, and to demonstrate the use of these clones as standardized reagents for a modified pathotyping assay by other laboratories. To date, MDV BAC clones have been produced for at least 10 MDV strains from all three serotypes including several virulent serotype 1 strains. We determined that MDV BAC clones exist for each virulent pathotype, despite the fact that these clones are not always equal in virulence to their corresponding parental strains. One clone from each pathotype was further evaluated in commercial specific-pathogen-free (SPF) chickens and found suitable for use in assays such as best-fit pathotyping, although results were variable based on the source of the SPF birds. The benefits of using BAC clones, which include easy shipping, ability to more easily manipulate, and long-term ability to use at a low passage level, are likely to result in the use of BAC clones as standard reagents for MD research. The use of the defined set of clones should allow side-by-side comparison, allowing researchers to better interpret results produced in different laboratories using different MDV field strains. Furthermore, a modified best-fit pathotyping assay has been proposed using these clones and reduced bird numbers.
[Mh] Termos MeSH primário: Cromossomos Artificiais Bacterianos/genética
Técnicas Genéticas
Herpesvirus Galináceo 2/isolamento & purificação
Doença de Marek/virologia
Patologia Molecular/métodos
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Galinhas
Técnicas Genéticas/normas
Herpesvirus Galináceo 2/classificação
Herpesvirus Galináceo 2/genética
Herpesvirus Galináceo 2/patogenicidade
Doença de Marek/patologia
Doenças das Aves Domésticas/patologia
Virulência
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1637/0005-2086-61.1.107


  6 / 1064 MEDLINE  
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[PMID]:28160668
[Au] Autor:Tai SS; Hearn C; Umthong S; Agafitei O; Cheng HH; Dunn JR; Niikura M
[Ad] Endereço:Faculty of Health Sciences, Simon Fraser University, Burnaby, BC, Canada.
[Ti] Título:Expression of Marek's Disease Virus Oncoprotein Meq During Infection in the Natural Host.
[So] Source:Virology;503:103-113, 2017 Mar.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gallid herpesvirus 2 (Marek's disease virus, MDV) causes lymphoproliferative Marek's disease (MD), and is unique among alphaherpesviruses as the viral genome encodes an oncoprotein, Meq. To elucidate the temporal relationship between Meq expression and the development of MD lymphomas in infected chickens, we generated a virulent recombinant MDV that expresses GFP simultaneously with Meq. By using this virus, we monitored the dynamics of Meq expression in vivo throughout the course of infection. In peripheral blood mononuclear cells, the percentage of Meq-expressing cells dramatically increased in the early latent phase but decreased thereafter. Furthermore, we discovered evidences that indicate some of the infected lymphocytes did not express Meq during the latent phase of MDV pathogenesis. These findings provide the first insight into the temporal relationship between Meq expression and MD progression, and new clues to refine the current MD pathogenesis model.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/imunologia
Herpesvirus Galináceo 2/patogenicidade
Doença de Marek/virologia
Proteínas Oncogênicas Virais/biossíntese
Proteínas Oncogênicas Virais/genética
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Transformação Celular Viral/genética
Embrião de Galinha
Galinhas/virologia
Proteínas de Fluorescência Verde/genética
Herpesvirus Galináceo 2/genética
Herpesvirus Galináceo 2/imunologia
Reação em Cadeia da Polimerase
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Eco-Q protein, Gallid herpesvirus 2); 0 (Oncogene Proteins, Viral); 0 (Recombinant Proteins); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE


  7 / 1064 MEDLINE  
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[PMID]:28043157
[Au] Autor:Suresh P; Johnson Rajeswar J; Sukumar K; Harikrishnan TJ; Srinivasan P
[Ad] Endereço:a Department of Veterinary Microbiology , Veterinary College and Research Institute , Namakkal , India.
[Ti] Título:Complete nucleotide sequence analysis of the oncogene "Meq" from serotype 1 Marek's disease virus isolates from India.
[So] Source:Br Poult Sci;58(2):111-115, 2017 Apr.
[Is] ISSN:1466-1799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:1. A study was undertaken to characterise the oncogene Meq at the molecular level for three serotype 1 Marek's disease virus (MDV) field isolates from vaccinated poultry flocks which had encountered a Marek's disease outbreak in the southern part of India. The isolates were named Ind/TN/11/01, Ind/KA/12/02 and Ind/TN/12/03. The oncogene Meq was amplified by PCR and sequenced. 2. The isolates were shown to have a homology for the Meq gene of 99.1-99.8% with various isolates from China and 98.5-99.2% with isolates from Europe and the USA. Alignment analysis of the nucleotide sequences showed that nucleotide mutations at 5 different positions in the Meq gene displayed perfect regularity in MDVs circulating in the southern part of India, which could be considered as features of field MDVs recently prevalent in this area. 3. In addition, the mutation in the Meq gene at positions 251, 260 and 437 was unique and coincides with very virulent strains from China GX0101, GXY2 and a Hungarian strain ATE. The mutation at positions 283 and 300 was unique and coincides with the very virulent strain ATE of Hungary. There were also single nucleotide mutations at positions 155 (A-T), 369 (A-C), 462 (C-A) and 548 (C-T) observed in the isolate Ind/TN/12/03. 4. Phylogenetic analysis of Meq sequences revealed that field MDVs in this area evolved independently but have similarities with very virulent strains from China, and that Meq has more similarities with the very virulent Hungarian strain.
[Mh] Termos MeSH primário: Galinhas
Herpesvirus Galináceo 2/genética
Doença de Marek/virologia
Proteínas Oncogênicas Virais/genética
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Índia
Mutação
Filogenia
Análise de Sequência de DNA/veterinária
Sorogrupo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Eco-Q protein, Gallid herpesvirus 2); 0 (Oncogene Proteins, Viral)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE
[do] DOI:10.1080/00071668.2016.1257780


  8 / 1064 MEDLINE  
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[PMID]:27975190
[Au] Autor:Demeke B; Jenberie S; Tesfaye B; Ayelet G; Yami M; Lamien CE; Gelaye E
[Ad] Endereço:Research and Development Department, National Veterinary Institute (NVI), P.O. Box 19, Debre zeit, Ethiopia.
[Ti] Título:Investigation of Marek's disease virus from chickens in central Ethiopia.
[So] Source:Trop Anim Health Prod;49(2):403-408, 2017 Feb.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Marek's disease (MD) is a lymphoproliferative and neuropathic disease of domestic chickens and less commonly, turkeys and quails, caused by a highly contagious, cell-associated, oncogenic herpesvirus. In Ethiopia, MD is believed to be introduced with importation of exotic and crossbred to improve the poultry production and has been reported to be a potential threat to the poultry sector both in backyard and commercial farming systems. This study was aimed at isolation and molecular analysis of MD virus isolates circulating in chicken population in the central part of Ethiopia where commercial farms are populated. From September 2013 to January 2014, clinical and post-mortem examination were conducted on diseased chickens suspected of MD virus infection. Representative spleen and feather follicle samples were collected following sterile procedure, and infectious virus isolation was performed using primary chicken fibroblast cell culture. Cell culture inoculated with suspension of pathological samples developed characteristic MD virus cytopathic effect of rounding of the cells and small plaques. Further analysis of the virus was conducted by conventional PCR amplifying the ICP4 gene fragment from eleven tissue samples using MD virus specific primers. PCR products were further sequenced and analyzed. Nucleotide sequence similarity search of the local isolates resulted a high degree of sequence similarity with Gallid Herpes virus type 2 strain (Marek's disease virus type 1, JN034558). To our knowledge, the present study is the first report conducted on virus isolation and molecular characterization of MD virus isolates circulated in Ethiopia. Eleven ICP4-like gene fragment (318 bp) sequences generated in the present study were uploaded in the public database (KU842366-76). Further research on virus isolation, genetic characterization, and infection dynamics is recommended targeting chickens of all age groups reared in different agro-ecological zones under different production system.
[Mh] Termos MeSH primário: Galinhas/virologia
Doença de Marek/virologia
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Autopsia
Sequência de Bases
Primers do DNA
Etiópia
Plumas/virologia
Herpesvirus Galináceo 2/genética
Doença de Marek/epidemiologia
Reação em Cadeia da Polimerase/veterinária
Aves Domésticas/virologia
Doenças das Aves Domésticas/epidemiologia
Análise de Sequência de DNA
Baço/virologia
Perus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-016-1208-1


  9 / 1064 MEDLINE  
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[PMID]:27942974
[Au] Autor:Andoh K; Yamazaki K; Honda Y; Honda T
[Ad] Endereço:Animal Pharmaceuticals Division, The Chemo-Sero-Therapeutic Research Institute, 1-6-1 Okubo, Kita-ku, Kumamoto, Kumamoto, 860-8568, Japan. andou-ki@kaketsuken.or.jp.
[Ti] Título:Turkey herpesvirus with an insertion in the UL3-4 region displays an appropriate balance between growth activity and antibody-eliciting capacity and is suitable for the establishment of a recombinant vaccine.
[So] Source:Arch Virol;162(4):931-941, 2017 Apr.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:We constructed turkey herpesvirus (HVT) vector vaccines in which the VP2 gene of infectious bursal disease virus (IBDV) was inserted into the HVT genome in the following regions: UL3-4, UL22-23, UL45-46, and US10-SORF3. We then evaluated the relationship between the gene insertion site and the capacity of the virus to elicit antibodies. rHVT/IBD (US10) showed good growth activity in vitro, with growth comparable to that of the parent HVT. On the other hand, rHVT/IBD (UL3-4), rHVT/IBD (UL22-23), and rHVT/IBD (UL45-46) exhibited decreased growth activity in chicken embryo fibroblast (CEF) cells compared to the parent HVT. However, the rHVT/IBD (US10) elicited lower levels of virus-neutralizing (VN) antibodies compared to the other constructs. rHVT/IBD (UL3-4) and rHVT/IBD (UL45-46) appeared to be similar in their ability to elicit VN antibodies. Based on the results of in vitro and in vivo assays, rHVT/IBD (UL3-4) was selected for further testing. In a challenge assay, rHVT/IBD (UL3-4) protected chickens from challenge with virulent Marek's disease virus serotype 1 and IBDV. In conclusion, the site of gene insertion may have a strong effect on the growth of the vector virus in vitro and its antibody-eliciting capacity. Insertions in the UL3-4 region permitted a balance between growth activity and VN-antibody-eliciting capacity, and this region might therefore be an appropriate insertion site for IBDV VP2.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Herpesvirus Meleagrídeo 1/imunologia
Doença de Marek/prevenção & controle
Doenças das Aves Domésticas/prevenção & controle
Proteínas Virais/imunologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Galinhas
Herpesvirus Meleagrídeo 1/química
Herpesvirus Meleagrídeo 1/genética
Herpesvirus Meleagrídeo 1/crescimento & desenvolvimento
Herpesvirus Galináceo 2/imunologia
Herpesvirus Galináceo 2/fisiologia
Doença de Marek/imunologia
Doença de Marek/virologia
Mutagênese Insercional
Doenças das Aves Domésticas/imunologia
Doenças das Aves Domésticas/virologia
Perus
Vacinação
Vacinas Sintéticas/administração & dosagem
Vacinas Sintéticas/química
Vacinas Sintéticas/genética
Vacinas Sintéticas/imunologia
Proteínas Virais/administração & dosagem
Proteínas Virais/química
Proteínas Virais/genética
Vacinas Virais/administração & dosagem
Vacinas Virais/química
Vacinas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Vaccines, Synthetic); 0 (Viral Proteins); 0 (Viral Vaccines)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3181-4


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[PMID]:27725354
[Au] Autor:Machida Y; Murata S; Matsuyama-Kato A; Isezaki M; Taneno A; Sakai E; Konnai S; Ohashi K
[Ad] Endereço:Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita-18, Nishi-9, Kita-ku, Sapporo 060-0818, Japan.
[Ti] Título:Isolation and purification of Gallid herpesvirus 2 strains currently distributed in Japan.
[So] Source:J Vet Med Sci;79(1):115-122, 2017 Jan 20.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Gallid herpesvirus 2 (GaHV-2) causes malignant lymphomas in chickens (Marek's disease, MD). Although MD is controlled through vaccination efforts, field isolates of GaHV-2 have increased in virulence worldwide and even cause MD in vaccinated chickens. GaHV-2 strains are classified into four categories (mild, virulent, very virulent and very virulent +) based on the virulence exhibited in experimental infection in unvaccinated or MD-vaccinated susceptible chickens. Although MD cases are sporadically reported in Japan, the recent field strains of GaHV-2 in Japan have not been characterized. During isolation of recent field strains by using primary chicken kidney cell cultures, a method classically used for GaHV-2 isolation, vaccine strains were simultaneously isolated. Therefore, it is necessary to separate vaccine strains to characterize the virulence and pathogenicity of the GaHV-2 strains currently distributed in Japan. In this study, we prepared cell suspensions from the spleens of MD-symptomatic chickens, inoculated day-old-chicks and isolated GaHV-2 strains by primary chicken kidney cell cultures at 2-3 weeks post inoculation. The isolated strains were passaged several times on chicken embryo fibroblast cells, and PCR analysis revealed that the isolated strains were not contaminated with vaccine strains. Moreover, the contaminant vaccine strains were completely removed by the purification of plaques observed in chicken kidney cells. These procedures are necessary to isolate GaHV-2 field strains from vaccine strains in order to carry out future studies to characterize these strains and glean insights into GaHV-2 virulence and pathogenicity.
[Mh] Termos MeSH primário: Galinhas/virologia
Herpesvirus Galináceo 2/isolamento & purificação
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Herpesvirus Galináceo 2/patogenicidade
Japão/epidemiologia
Rim/citologia
Doença de Marek/virologia
Vacinas contra Doença de Marek
Reação em Cadeia da Polimerase
Baço/virologia
Cultura de Vírus/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Marek Disease Vaccines)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161012
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.16-0329



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