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  1 / 102 MEDLINE  
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Roehe, Paulo M
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[PMID]:27693624
[Au] Autor:Siedler BS; Roloff BC; de Sá GL; Neis A; Conceição FR; Hartwig DD; Borsuk S; Dellagostin OA; Campos FS; Roehe PM; Hartleben CP; McBride AJ
[Ad] Endereço:Núcleo de Biotecnologia - CDTec, Universidade Federal de Pelotas, Campus Universitário, Caixa Postal 354, 96010-900, Pelotas, RS, Brazil. Electronic address: bssiedler@gmail.com.
[Ti] Título:Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle.
[So] Source:Protein Expr Purif;130:21-27, 2017 Feb.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bovine herpesvirus (BoHV) glycoprotein E (gE) is a non-essential envelope glycoprotein and the deletion of gE has been used to develop BoHV-1 and BoHV-5 differential vaccine strains. The DIVA (Differentiation of Infected from Vaccinated Animals) strategy, using marker vaccines based on gE-negative BoHV strains, allows the identification of vaccinated or infected animals in immunoassays designed to detect anti-gE antibodies. In this study a codon optimized synthetic sequence of gE containing highly conserved regions from BoHV-1 and BoHV-5 was expressed in Pichia pastoris. Following expression, the recombinant gE (rgE) was secreted and purified from the culture medium. The rgE was identified by Western blotting (WB) using sera from cattle naturally infected with BoHV-1 and/or BoHV-5, or sera from bovines experimentally infected with wild-type BoHV-5. Sera collected from cattle vaccinated with a BoHV-5 gI/gE/US9¯ marker vaccine failed to recognise rgE. Expression of rgE, based on a sequence containing highly conserved regions from BoHV-1 and BoHV-5, in P. pastoris enabled the production of large quantities of rgE suitable for use in immunoassays for the differentiation vaccinated or infected cattle.
[Mh] Termos MeSH primário: Expressão Gênica
Herpesvirus Bovino 1/genética
Herpesvirus Bovino 5
Rinotraqueíte Infecciosa Bovina
Pichia/metabolismo
Proteínas do Envelope Viral
Proteínas Virais
[Mh] Termos MeSH secundário: Animais
Bovinos
Herpesvirus Bovino 1/metabolismo
Vacinas contra Herpesvirus/farmacologia
Rinotraqueíte Infecciosa Bovina/sangue
Rinotraqueíte Infecciosa Bovina/diagnóstico
Pichia/genética
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/secreção
Proteínas do Envelope Viral/química
Proteínas do Envelope Viral/genética
Proteínas do Envelope Viral/isolamento & purificação
Proteínas do Envelope Viral/secreção
Proteínas Virais/química
Proteínas Virais/genética
Proteínas Virais/isolamento & purificação
Proteínas Virais/secreção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herpesvirus Vaccines); 0 (Recombinant Proteins); 0 (Viral Envelope Proteins); 0 (Viral Proteins); 0 (bovine herpesvirus type-1 glycoproteins); 0 (glycoprotein E, bovine herpesvirus type 5)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161004
[St] Status:MEDLINE


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[PMID]:27938713
[Au] Autor:Cardoso TC; Ferreira HL; Okamura LH; Giroto TP; Oliveira BR; Fabri CU; Gameiro R; Flores EF
[Ad] Endereço:Laboratory of Animal Virology and Cell Culture, College of Veterinary Medicine, Univ - Universidade Estadual Paulista, Araçatuba, São Paulo 16050-680, Brazil. Electronic address: tcardoso@fmva.unesp.br.
[Ti] Título:Cellular response markers and cytokine gene expression in the central nervous system of cattle naturally infected with bovine herpesvirus 5.
[So] Source:Vet J;218:71-77, 2016 Dec.
[Is] ISSN:1532-2971
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study reports an investigation on the phenotype of inflammatory and immune cells, cytokine and viral gene expression in the brains of cattle naturally infected with bovine herpesvirus 5 (BHV5). Brain sections of 38 affected animals were analysed for the nature and extent of perivascular cuffs in the Virchow-Robin space and parenchyma. Histopathological changes were severe in the olfactory bulbs (Obs), hippocampus, piriform, frontal, temporal and parietal cortices/lobes and were characterized by inflammatory infiltrates in Virchow-Robin spaces. The histopathological changes correlated positively with the distribution of BHV5 antigens (r = 0.947; P < 0.005). Cells of CD3+ phenotype were predominant in areas with severe perivascular cuffs. Viral antigens and genomic viral DNA were detected in the Obs and piriform lobe, simultaneously (r = 0.987; P < 0.005). Similarly, pro-inflammatory cytokine genes INFG, IL2, TNF and LTBR were expressed in the same brain areas (P < 0.005). These results provide important information on the inflammatory and immunological events accompanying BHV5 neurological infections. Our findings provide the first evidence for increased immune activation followed by inflammatory cytokine expression, positively correlated with viral replication in the cranial areas of the brain. Taken together, these results suggest that the host immune response and inflammation play a crucial role in the pathogenesis of acute encephalitis by BHV5 in cattle.
[Mh] Termos MeSH primário: Doenças dos Bovinos/imunologia
Encefalite Viral/veterinária
Infecções por Herpesviridae/veterinária
Herpesvirus Bovino 5/fisiologia
Meningoencefalite/veterinária
[Mh] Termos MeSH secundário: Animais
Antígenos Virais/metabolismo
Biomarcadores/metabolismo
Bovinos
Doenças dos Bovinos/virologia
Sistema Nervoso Central/patologia
Citocinas/genética
Citocinas/metabolismo
Encefalite Viral/imunologia
Encefalite Viral/virologia
Expressão Gênica
Genoma Viral
Infecções por Herpesviridae/imunologia
Infecções por Herpesviridae/virologia
Meningoencefalite/imunologia
Meningoencefalite/virologia
Distribuição Tecidual
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Viral); 0 (Biomarkers); 0 (Cytokines)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


  3 / 102 MEDLINE  
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[PMID]:27659677
[Au] Autor:Silvestro C; Bratanich A
[Ad] Endereço:Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Avenida Chorroarín 280, 1427 CABA, Buenos Aires, Argentina.
[Ti] Título:The latency related gene of bovine herpesvirus types 1 and 5 and its modulation of cellular processes.
[So] Source:Arch Virol;161(12):3299-3308, 2016 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bovine herpesvirus type 1 (BoHV-1) and bovine herpesvirus type 5 (BoHV-5) are important pathogens of cattle. The diseases they produce are quite different, with BoHV-5 being more neuropathogenic than BoHV-1 which mainly induces respiratory symptoms. The sequencing of the entire BoHV-5 genome has shown that most of the differences between these viruses are found in the immediate early and LR (latency related) genes. The LR gene is the only viral gene abundantly expressed in latently infected neurons, is essential for viral reactivation and seems to have an anti-apoptotic function which can be observed in vivo and in vitro. This gene spans two potential ORFs (1 and 2) which can also be found as a fused version, an ORF-E protein encoded within the promoter region and two miRNAs located within the 5' UTR segment. Most of the essential functions of the LR gene seem to be located within the ORF-2 which has been found to modulate components of cell signaling/cycle pathways. In this review we present a comparative sequence analysis of the LR gene of several BoHV-5 isolates, their differences with the BoHV-1 homologue and the potential impact this may have on its function. The LR gene was found to be highly conserved in all sequenced BoHV-5 strains. ORF-1 shares 60 % homology compared to BoHV-1 whereas the BoHV-5 homologue of ORF-2 is truncated at amino acid 51. Preliminary studies analyzing the emerging transcripts from the BoHV-5 LR gene in infected cells, as well as in stably transfected cells, indicates that their products are, in fact, missing crucial components of the anti-apoptotic function when compared to the BoHV-1 LR gene. In addition these transcripts maintain a region that, similar to what is found in BoHV-1, would produce a miRNA with the potential to recognize a region within the BoHV-5 immediate early gene. All together, these BoHV-5 characteristics suggest that this virus would not possess the same repertoire of latency maintaining functions as BoHV-1. Implications for BoHV-5 neuropathogenic potential are discussed.
[Mh] Termos MeSH primário: Herpesvirus Bovino 1/genética
Herpesvirus Bovino 1/fisiologia
Herpesvirus Bovino 5/genética
Herpesvirus Bovino 5/fisiologia
Interações Hospedeiro-Patógeno
Proteínas Virais/genética
Latência Viral
[Mh] Termos MeSH secundário: Sequência Conservada
Variação Genética
Análise de Sequência de DNA
Transcrição Genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160924
[St] Status:MEDLINE


  4 / 102 MEDLINE  
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[PMID]:27477504
[Au] Autor:Aparecida Silva Barbosa A; Freitas Versiani A; Fonseca da Cunha Sousa L; Silva de Miranda A; Gasparini MR; Brant F; Silva DG; Luisa Quintino-de-Carvalho I; Marianetti Soriani F; Guimarães da Fonseca F; César Vasconcelos A; da Silva Barcelos L; Martins Teixeira M; Lúcio Teixeira A; Machado FS; Barbosa-Stancioli EF; Rachid MA
[Ad] Endereço:Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, MG, Brazil. Electronic address: alinesb.bio@gmail.com.
[Ti] Título:Role of the suppressor of cytokine signaling 2 (SOCS2) during meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5).
[So] Source:Comp Immunol Microbiol Infect Dis;47:26-31, 2016 Aug.
[Is] ISSN:1878-1667
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The role of suppressors of cytokine signaling (SOCS) in meningoencephalitis caused by Bovine herpesvirus 5 (BoHV-5) was evaluated by intracranial infection in C57BL/6 wild-type mice (WT) and SOCS2 deficient mice (SOCS2(-/-)). Both infected groups presented weight loss, ruffled fur and hunched posture. Additionally, infected SOCS2(-/-) mice showed swollen chamfer and progressive depression. Infected WT animals developed mild meningitis, characterized by infiltration of mononuclear cells. Moreover, viral DNA was detected in liver and lung from infected WT group. This group also showed elevated brain levels of IFN-γ, IL-10, CXCL1 and CCL5, when compared with non-infected WT animals. Brain inflammation was exacerbated in infected SOCS2(-/-) mice with widespread distribution of the virus and increased brain levels of TNF-α, IFN-γ, IL-10, IL-12, CXCL1 and CCL5, when compared with WT infected mice. Moreover, infected SOCS2 deficient mice exhibited reduced brain mRNA expression of IFNα and IFNß and increased expression of mRNA of SOCS1, compared with infected WT mice. Taken together, our study provides an insight into the role of SOCS2 in modulating the immune response to BoHV-5 infection.
[Mh] Termos MeSH primário: Encéfalo/virologia
Infecções por Herpesviridae/veterinária
Herpesvirus Bovino 5/genética
Herpesvirus Bovino 5/patogenicidade
Meningoencefalite/metabolismo
Proteínas Supressoras da Sinalização de Citocinas/genética
Proteínas Supressoras da Sinalização de Citocinas/fisiologia
[Mh] Termos MeSH secundário: Animais
Encéfalo/imunologia
Encéfalo/fisiopatologia
Bovinos
Quimiocina CCL5/genética
Quimiocina CXCL1/genética
Citocinas/genética
DNA Viral
Infecções por Herpesviridae/imunologia
Infecções por Herpesviridae/fisiopatologia
Infecções por Herpesviridae/virologia
Herpesvirus Bovino 5/imunologia
Interferon-alfa/genética
Interferon beta/genética
Fígado/virologia
Pulmão/virologia
Meningoencefalite/imunologia
Meningoencefalite/fisiopatologia
Meningoencefalite/virologia
Camundongos
Camundongos Endogâmicos C57BL
Reação em Cadeia da Polimerase
Proteínas Supressoras da Sinalização de Citocinas/deficiência
Proteínas Supressoras da Sinalização de Citocinas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ccl5 protein, mouse); 0 (Chemokine CCL5); 0 (Chemokine CXCL1); 0 (Cxcl1 protein, mouse); 0 (Cytokines); 0 (DNA, Viral); 0 (Ifna1 protein, mouse); 0 (Interferon-alpha); 0 (Socs2 protein, mouse); 0 (Suppressor of Cytokine Signaling Proteins); 77238-31-4 (Interferon-beta)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170120
[Lr] Data última revisão:
170120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160802
[St] Status:MEDLINE


  5 / 102 MEDLINE  
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[PMID]:27473967
[Au] Autor:Rensetti D; Marin M; Quintana S; Morán P; Verna A; Odeón A; Pérez S
[Ad] Endereço:Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Paraje Arroyo Seco S/N, Tandil (7000), Argentina.
[Ti] Título:Involvement of toll-like receptors 3 and 7/8 in the neuropathogenesis of bovine herpesvirus types 1 and 5.
[So] Source:Res Vet Sci;107:1-7, 2016 Aug.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are closely related alpha-herpesviruses. BoHV-5 is the causal agent of non-suppurative meningoencephalitis in calves. BoHV-1 causes respiratory disease, abortions, genital disorders and, occasionally, encephalitis in cattle. Both viruses are neurotropic and they share similar biological properties. Nevertheless, they differ in their ability to cause neurological disease. Toll-like receptors (TLRs) are involved in the innate immune response to pathogens. In this study, the variations in the expression levels of TLRs were evaluated in different regions of the bovine central nervous system during the acute infection and reactivation of BoHV-1 and BoHV-5- infected cattle. With the exception of TLR9, significant up-regulation of all TLRs was detected following primary infection of neural tissues by both bovine alpha-herpesviruses. Furthermore, the stages of acute infection and reactivation were characterized by a distinguishable TLR expression pattern. Important differences in TLR expression upon infection of the central nervous system by BoHV-1 or BoHV-5 were not detected. The striking differences in TLR mRNA levels during acute infection and reactivation provide evidence that the innate immune response may be involved in the clinical outcomes observed at each stage. Further research is required to analyze the mechanisms that initiate TLR activation and the signaling cascade mediated by each TLR to elucidate the precise role these receptors play in bovine herpesvirus encephalitis.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Encefalite Viral/veterinária
Infecções por Herpesviridae/veterinária
Herpesvirus Bovino 1
Herpesvirus Bovino 5
Receptores Toll-Like/metabolismo
[Mh] Termos MeSH secundário: Animais
Encéfalo/patologia
Encéfalo/virologia
Bovinos
Doenças dos Bovinos/patologia
Encefalite Viral/patologia
Encefalite Viral/virologia
Feminino
Regulação Viral da Expressão Gênica
Infecções por Herpesviridae/patologia
Infecções por Herpesviridae/virologia
Herpesvirus Bovino 1/isolamento & purificação
Herpesvirus Bovino 5/isolamento & purificação
Imunidade Inata
Gravidez
Distribuição Aleatória
Transdução de Sinais
Receptor 3 Toll-Like/genética
Receptor 3 Toll-Like/metabolismo
Receptor 7 Toll-Like/genética
Receptor 7 Toll-Like/metabolismo
Receptor 8 Toll-Like/genética
Receptor 8 Toll-Like/metabolismo
Receptores Toll-Like/genética
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Toll-Like Receptor 3); 0 (Toll-Like Receptor 7); 0 (Toll-Like Receptor 8); 0 (Toll-Like Receptors)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170203
[Lr] Data última revisão:
170203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160731
[St] Status:MEDLINE


  6 / 102 MEDLINE  
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Roehe, Paulo Michel
PubMed Central Texto completo
Texto completo
[PMID]:27224314
[Au] Autor:Puentes R; Campos FS; Furtado A; Torres FD; Franco AC; Maisonnave J; Roehe PM
[Ad] Endereço:Departamento de Ciencias Microbiológicas, Área de Inmunología, Facultad de Veterinaria, Universidad de la República Oriental del Uruguay (UdelaR), Montevideo, Uruguay.
[Ti] Título:Comparison between DNA Detection in Trigeminal Nerve Ganglia and Serology to Detect Cattle Infected with Bovine Herpesviruses Types 1 and 5.
[So] Source:PLoS One;11(5):e0155941, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bovine herpesviruses (BoHVs) types 1 (BoHV-1) and 5 (BoHV-5) are alphaherpesviruses of major importance to the bovine production chain. Such viruses are capable of establishing latent infections in neuronal tissues. Infected animals tend to develop a serological response to infection; however, such response-usually investigated by antibody assays in serum-may eventually not be detected in laboratory assays. Nevertheless, serological tests such as virus neutralization (VN) and various enzyme-linked immunosorbent assays (ELISAs) are widely employed to check individual or herd status of BoHV infections. The correlation between detection of antibodies and the presence of viral nucleic acids as indicatives of infection in infected cattle has not been deeply examined. In order to investigate such correlation, 248 bovine serum samples were tested by VN to BoHV-1 and BoHV-5, as well as in a widely employed (though not type-differential) gB ELISA (IDEXX IBR gB X2 Ab Test) in search for antibodies to BoHVs. Immediately after blood withdrawal, cattle were slaughtered and trigeminal ganglia (TG) excised for DNA extraction and viral nucleic acid detection (NAD) by nested PCR. Neutralizing antibodies to BoHV-1 and/or BoHV-5 were detected in 44.8% (111/248) of sera, whereas the gB ELISA detected antibodies in 51.2% (127/248) of the samples. However, genomes of either BoHV-1, BoHV-5, or both, were detected in TGs of 85.9% (213/248) of the animals. These findings reveal that the assays designed to detect antibodies to BoHV-1 and/or BoHV-5 employed here may fail to detect a significant number of latently infected animals (in this study, 35.7%). From such data, it is clear that antibody assays are poorly correlated with detection of viral genomes in BoHV-1 and BoHV-5-infected animals.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Doenças dos Bovinos
DNA Viral/genética
Encefalite Viral
Infecções por Herpesviridae
Herpesvirus Bovino 1
Herpesvirus Bovino 5
Meningoencefalite
Gânglio Trigeminal/virologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/diagnóstico
Doenças dos Bovinos/genética
Doenças dos Bovinos/imunologia
Linhagem Celular
Encefalite Viral/diagnóstico
Encefalite Viral/genética
Encefalite Viral/imunologia
Encefalite Viral/veterinária
Ensaio de Imunoadsorção Enzimática/métodos
Infecções por Herpesviridae/diagnóstico
Infecções por Herpesviridae/genética
Infecções por Herpesviridae/imunologia
Infecções por Herpesviridae/veterinária
Herpesvirus Bovino 1/genética
Herpesvirus Bovino 1/imunologia
Herpesvirus Bovino 5/genética
Herpesvirus Bovino 5/imunologia
Meningoencefalite/diagnóstico
Meningoencefalite/genética
Meningoencefalite/imunologia
Meningoencefalite/veterinária
Reação em Cadeia da Polimerase/métodos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (DNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170706
[Lr] Data última revisão:
170706
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0155941


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[PMID]:27033908
[Au] Autor:Marin MS; Quintana S; Leunda MR; Odeón AC; Pérez SE
[Ad] Endereço:Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ, Buenos Aires, Argentina.
[Ti] Título:Distribution of bovine alpha-herpesviruses and expression of toll-like receptors in the respiratory system of experimentally infected calves.
[So] Source:Res Vet Sci;105:53-5, 2016 Apr.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study provides an initial analysis of the toll-like receptors (TLRs) that might be implicated in alpha-herpesvirus infection of the bovine respiratory system. A significant variation in the expression of TLR3 and TLRs 7-9 during bovine herpesvirus type 1 (BoHV-1) and 5 (BoHV-5) acute infections and particularly an up-regulation during viral reactivation in respiratory tissues has been demonstrated. Furthermore, viral distribution in the respiratory tract of BoHV-1- and BoHV-5-infected calves at different stages of the infectious cycle was analysed. The wide distribution of BoHV DNA in the respiratory tract during acute infection was restricted during latent infection and the subsequent reactivation of BoHV-1 and BoHV-5. Overall, the findings presented here contribute to the knowledge on the replication and dissemination of bovine alpha-herpesviruses. Furthermore, some of the immune factors triggered in the host that determine the different outcomes of infection by two closely related pathogens of cattle have been elucidated.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Regulação da Expressão Gênica/imunologia
Herpesvirus Bovino 1/metabolismo
Herpesvirus Bovino 5/metabolismo
Rinotraqueíte Infecciosa Bovina/virologia
Receptores Toll-Like/metabolismo
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/metabolismo
Herpesvirus Bovino 1/genética
Herpesvirus Bovino 5/genética
Rinotraqueíte Infecciosa Bovina/imunologia
Sistema Respiratório/metabolismo
Receptores Toll-Like/genética
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Toll-Like Receptors)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE


  8 / 102 MEDLINE  
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Roehe, Paulo M
PubMed Central Texto completo
Texto completo
[PMID]:26866923
[Au] Autor:Dummer LA; Araujo IL; Campos FS; da Rosa MC; Finger PF; de Oliveira PD; Conceição FR; Fischer G; Roehe PM; Leite FP
[Ad] Endereço:Laboratório de Bacteriologia, Centro de Desenvolvimento Tecnológico - Núcleo de Biotecnologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
[Ti] Título:Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5.
[So] Source:PLoS One;11(2):e0149134, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) are economically important pathogens, associated with a variety of clinical syndromes, including respiratory and genital disease, reproductive failure and meningoencephalitis. The standard serological assay to diagnose BoHV-1 and BoHV-5 infections is the virus neutralization test (VNT), a time consuming procedure that requires manipulation of infectious virus. In the present study a highly sensitive and specific single dilution indirect ELISA was developed using recombinant glycoprotein D from BoHV-5 as antigen (rgD5ELISA). Bovine serum samples (n = 450) were screened by VNT against BoHV-5a and by rgD5ELISA. Compared with the VNT, the rgD5ELISA demonstrated accuracy of 99.8%, with 100% sensitivity, 96.7% specificity and coefficient of agreement between the tests of 0.954. The rgD5ELISA described here shows excellent agreement with the VNT and is shown to be a simple, convenient, specific and highly sensitive virus-free assay for detection of serum antibodies to BoHV-5.
[Mh] Termos MeSH primário: Doenças dos Bovinos/diagnóstico
Encefalite Viral/diagnóstico
Ensaio de Imunoadsorção Enzimática/métodos
Infecções por Herpesviridae/diagnóstico
Meningoencefalite/diagnóstico
Proteínas do Envelope Viral/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Área Sob a Curva
Bovinos
Doenças dos Bovinos/virologia
Cães
Encefalite Viral/imunologia
Geografia
Infecções por Herpesviridae/imunologia
Herpesvirus Bovino 5
Células Madin Darby de Rim Canino
Meningoencefalite/imunologia
Testes de Neutralização
Pichia
Curva ROC
Proteínas Recombinantes/química
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Proteínas do Envelope Viral/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Recombinant Proteins); 0 (Viral Envelope Proteins); 0 (glycoprotein D, bovine herpesvirus 5)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160227
[Lr] Data última revisão:
160227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160212
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0149134


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[PMID]:26478539
[Au] Autor:Marin MS; Quintana S; Leunda MR; Recavarren M; Pagnuco I; Späth E; Pérez S; Odeón A
[Ad] Endereço:Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, C1033AAJ Buenos Aires, Argentina.
[Ti] Título:A new method for simultaneous detection and discrimination of Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) using real time PCR with high resolution melting (HRM) analysis.
[So] Source:J Virol Methods;227:14-22, 2016 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are antigenically and genetically similar. The aim of this study was to develop a simple and reliable one-step real time PCR assay with high resolution melting (HRM) analysis for the simultaneous detection and differentiation of BoHV-1 and BoHV-5. Optimization of assay conditions was performed with DNA from reference strains. Then, DNA from field isolates, clinical samples and tissue samples of experimentally infected animals were studied by real time PCR-HRM. An efficient amplification of real time PCR products was obtained, and a clear melting curve and appropriate melting peaks for both viruses were achieved in the HRM curve analysis for BoHV type identification. BoHV was identified in all of the isolates and clinical samples, and BoHV types were properly differentiated. Furthermore, viral DNA was detected in 12/18 and 7/18 samples from BoHV-1- and BoHV-5-infected calves, respectively. Real time PCR-HRM achieved a higher sensitivity compared with virus isolation or conventional PCR. In this study, HRM was used as a novel procedure. This method provides rapid, sensitive, specific and simultaneous detection of bovine alpha-herpesviruses DNA. Thus, this technique is an excellent tool for diagnosis, research and epidemiological studies of these viruses in cattle.
[Mh] Termos MeSH primário: Infecções por Herpesviridae/diagnóstico
Infecções por Herpesviridae/virologia
Herpesvirus Bovino 1/isolamento & purificação
Herpesvirus Bovino 5/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real
Virologia/métodos
[Mh] Termos MeSH secundário: Animais
Bovinos
Cães
Herpesvirus Bovino 1/genética
Herpesvirus Bovino 5/genética
Células Madin Darby de Rim Canino
Desnaturação de Ácido Nucleico
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1606
[Cu] Atualização por classe:151209
[Lr] Data última revisão:
151209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151020
[St] Status:MEDLINE


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[PMID]:26239341
[Au] Autor:Cardoso TC; Ferreira HL; Okamura LH; Oliveira BR; Rosa AC; Gameiro R; Flores EF
[Ad] Endereço:Laboratory of Animal Virology and Cell Culture, Universidade Estadual Paulista, Araçatuba, SP, 16050-680, Brazil. tcardoso@fmva.unesp.br.
[Ti] Título:Comparative analysis of the replication of bovine herpesvirus 1 (BHV1) and BHV5 in bovine-derived neuron-like cells.
[So] Source:Arch Virol;160(11):2683-91, 2015 Nov.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Members of the subfamily Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as preferential sites for primary replication. However, bovine herpesvirus 5 (BoHV5) is neurotropic and neuroinvasive and responsible for meningoencephalitis in cattle and in animal models. A related virus, BoHV1 has also been occasionally implicated in natural cases of neurological infection and disease in cattle. The aim of the present study was to assess the in vitro effects of BoHV1 and BoHV5 replication in neuron-like cells. Overall, cytopathic effects, consisting of floating rounded cells, giant cells and monolayer lysis, induced by both viruses at 48 h postinfection (p.i.) resulted in a loss of cell viability and high virus titres (r = 0.978). The BoHV1 Cooper strain produced the lowest titres in neuron-like cells, although viral DNA was detected in infected cells during all experiments. Virus replication in infected cells was demonstrated by immunocytochemistry, flow cytometry and qPCR assays. BoHV antigens were better visualized at 48 h p.i. and flow cytometry analysis showed that SV56/90 and Los Angeles antigens were present at higher levels. In spite of the fact that BoHV titres dropped at 48 h p.i, viral DNA remained detectable until 120 h p.i. Sensitive TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) and annexin V assays were used to identify apoptosis. BoHV5 induced death in approximately 50% of cells within 24 h p.i., similar to what has been observed for BoHV1 Los Angeles. Infection with the BoHV1 Cooper strain resulted in 26.37% of cells being in the early stages of apoptosis; 63.69% of infected cells were considered viable. Modulation of mitochondrial function, as measured by mitochondrial membrane depolarization, was synchronous with the virus replication cycle, cell viability and virus titres at 48 h p.i. Our results indicate that apoptosis plays an important role in preventing neuronal death and provides a bovine-derived in vitro system to study herpesvirus-neuron interactions.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Infecções por Herpesviridae/veterinária
Herpesvirus Bovino 1/fisiologia
Herpesvirus Bovino 5/fisiologia
Neurônios/virologia
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Apoptose
Bovinos
Doenças dos Bovinos/fisiopatologia
Células Cultivadas
Infecções por Herpesviridae/fisiopatologia
Infecções por Herpesviridae/virologia
Herpesvirus Bovino 1/genética
Herpesvirus Bovino 5/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1605
[Cu] Atualização por classe:151106
[Lr] Data última revisão:
151106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150805
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-015-2537-5



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