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Pesquisa : B04.280.382.100.900.430 [Categoria DeCS]
Referências encontradas : 1031 [refinar]
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  1 / 1031 MEDLINE  
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[PMID]:29269546
[Au] Autor:Strang C; Newton R
[Ti] Título:Control and disease clearance after neurological EHV-1 in the UK.
[So] Source:Vet Rec;181(25):678-679, 2017 12 23.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Surtos de Doenças/veterinária
Infecções por Herpesviridae/veterinária
Herpesvirus Equídeo 1/isolamento & purificação
Doenças dos Cavalos/prevenção & controle
Vigilância de Evento Sentinela/veterinária
[Mh] Termos MeSH secundário: Animais
Surtos de Doenças/prevenção & controle
Infecções por Herpesviridae/epidemiologia
Infecções por Herpesviridae/prevenção & controle
Doenças dos Cavalos/epidemiologia
Cavalos
Masculino
Medição de Risco
Reino Unido/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171223
[St] Status:MEDLINE
[do] DOI:10.1136/vr.j5906


  2 / 1031 MEDLINE  
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[PMID]:28631601
[Au] Autor:Holz CL; Nelli RK; Wilson ME; Zarski LM; Azab W; Baumgardner R; Osterrieder N; Pease A; Zhang L; Hession S; Goehring LS; Hussey SB; Soboll Hussey G
[Ad] Endereço:1​Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
[Ti] Título:Viral genes and cellular markers associated with neurological complications during herpesvirus infections.
[So] Source:J Gen Virol;98(6):1439-1454, 2017 Jun.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Despite the importance of neurological disorders associated with herpesviruses, the mechanism by which these viruses influence the central nervous system (CNS) has not been definitively established. Owing to the limitations of studying neuropathogenicity of human herpesviruses in their natural host, many aspects of their pathogenicity and immune response are studied in animal models. Here, we present an important model system that enables studying neuropathogenicity of herpesviruses in the natural host. Equine herpesvirus type 1 (EHV-1) is an alphaherpesvirus that causes a devastating neurological disease (EHV-1 myeloencephalopathy; EHM) in horses. Like other alphaherpesviruses, our understanding of virus neuropathogenicity in the natural host beyond the essential role of viraemia is limited. In particular, information on the role of different viral proteins for virus transfer to the spinal cord endothelium in vivo is lacking. In this study, the contribution of two viral proteins, DNA polymerase (ORF30) and glycoprotein D (gD), to the pathogenicity of EHM was addressed. Furthermore, different cellular immune markers, including alpha-interferon (IFN-α), gamma-interferon (IFN-γ), interleukin-10 (IL-10) and interleukin-1 beta (IL-1ß), were identified to play a role during the course of the disease.
[Mh] Termos MeSH primário: Biomarcadores/análise
Encefalite Viral/patologia
Infecções por Herpesviridae/complicações
Infecções por Herpesviridae/virologia
Herpesvirus Equídeo 1/patogenicidade
Interações Hospedeiro-Patógeno
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Feminino
Infecções por Herpesviridae/patologia
Cavalos
Masculino
Modelos Animais
Fatores de Virulência/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Viral Proteins); 0 (Virulence Factors)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000773


  3 / 1031 MEDLINE  
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[PMID]:28439710
[Au] Autor:El-Husseini DM; Helmy NM; Tammam RH
[Ad] Endereço:Biotechnology Department, Animal Health Research Institute, Giza, Egypt. Dalia_biotech@yahoo.com.
[Ti] Título:Application of gold nanoparticle-assisted PCR for equine herpesvirus 1 diagnosis in field samples.
[So] Source:Arch Virol;162(8):2297-2303, 2017 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Equine herpesvirus 1 (EHV-1) is one of the most significant pathogens that affects equine species worldwide, causing sporadic abortion, neonatal deaths, chorioretinopathy, as well as neurological and upper respiratory tract diseases. Currently, conventional PCR targeting different genes is used widely for the molecular detection of EHV-1, but the low viral titer in some clinical samples can lead to false negative results. In this study, we aimed to assess gold nanoparticle (GNP)-assisted PCR as an inexpensive, highly efficient, and sensitive method for the detection of EHV-1, and to compare its results with conventional PCR and real-time quantitative PCR (qPCR). Out of 83 field samples, 28.9%, 26.5%, and 15.6% were EHV-1-positive by qPCR, GNP-assisted PCR and conventional PCR, respectively. All three techniques specifically target the viral glycoprotein B gene. The optimized GNP-assisted PCR showed no cross-reactivity with EHV-1-negative samples (diagnosed by qPCR). GNP-assisted PCR is a powerful new tool for EHV-1 detection and surveillance, because of its simplicity, sensitivity and specificity. It can be used as an alternative to qPCR in laboratories that cannot afford the expense of a qPCR system.
[Mh] Termos MeSH primário: Infecções por Herpesviridae/veterinária
Herpesvirus Equídeo 1/isolamento & purificação
Doenças dos Cavalos/diagnóstico
Cavalos/virologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Animais
DNA Viral/isolamento & purificação
Ouro
Infecções por Herpesviridae/diagnóstico
Doenças dos Cavalos/virologia
Nanopartículas
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Sensibilidade e Especificidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 7440-57-5 (Gold)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3379-0


  4 / 1031 MEDLINE  
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[PMID]:28404844
[Au] Autor:Liu SA; Stanfield BA; Chouljenko VN; Naidu S; Langohr I; Del Piero F; Ferracone J; Roy AA; Kousoulas KG
[Ad] Endereço:Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA.
[Ti] Título:Intramuscular Immunization of Mice with the Live-Attenuated Herpes Simplex Virus 1 Vaccine Strain VC2 Expressing Equine Herpesvirus 1 (EHV-1) Glycoprotein D Generates Anti-EHV-1 Immune Responses in Mice.
[So] Source:J Virol;91(12), 2017 Jun 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vaccination remains the best option to combat equine herpesvirus 1 (EHV-1) infection, and several different strategies of vaccination have been investigated and developed over the past few decades. Herein, we report that the live-attenuated herpes simplex virus 1 (HSV-1) VC2 vaccine strain, which has been shown to be unable to enter into neurons and establish latency in mice, can be utilized as a vector for the heterologous expression of EHV-1 glycoprotein D (gD) and that the intramuscular immunization of mice results in strong antiviral humoral and cellular immune responses. The VC2-EHV-1-gD recombinant virus was constructed by inserting an EHV-1 gD expression cassette under the control of the cytomegalovirus immediate early promoter into the VC2 vector in place of the HSV-1 thymidine kinase (UL23) gene. The vaccines were introduced into mice through intramuscular injection. Vaccination with both the VC2-EHV-1-gD vaccine and the commercially available vaccine Vetera EHV 1/4 (Vetera; Boehringer Ingelheim Vetmedica) resulted in the production of neutralizing antibodies, the levels of which were significantly higher in comparison to those in VC2- and mock-vaccinated animals ( < 0.01 or < 0.001). Analysis of EHV-1-reactive IgG subtypes demonstrated that vaccination with the VC2-EHV-1-gD vaccine stimulated robust IgG1 and IgG2a antibodies after three vaccinations ( < 0.001). Interestingly, Vetera-vaccinated mice produced significantly higher levels of IgM than mice in the other groups before and after challenge ( < 0.01 or < 0.05). Vaccination with VC2-EHV-1-gD stimulated strong cellular immune responses, characterized by the upregulation of both interferon- and tumor necrosis factor-positive CD4 T cells and CD8 T cells. Overall, the data suggest that the HSV-1 VC2 vaccine strain may be used as a viral vector for the vaccination of horses as well as, potentially, for the vaccination of other economically important animals. A novel virus-vectored VC2-EHV-1-gD vaccine was constructed using the live-attenuated HSV-1 VC2 vaccine strain. This vaccine stimulated strong humoral and cellular immune responses in mice, suggesting that it could protect horses against EHV-1 infection.
[Mh] Termos MeSH primário: Infecções por Herpesviridae/veterinária
Herpesvirus Equídeo 1/química
Herpesvirus Equídeo 1/imunologia
Vacinas contra Herpesvirus/imunologia
Doenças dos Cavalos/prevenção & controle
Proteínas do Envelope Viral/genética
[Mh] Termos MeSH secundário: Animais
Anticorpos Neutralizantes/sangue
Anticorpos Neutralizantes/imunologia
Anticorpos Antivirais/sangue
Anticorpos Antivirais/imunologia
Linfócitos T CD4-Positivos
Linfócitos T CD8-Positivos
Modelos Animais de Doenças
Infecções por Herpesviridae/imunologia
Infecções por Herpesviridae/prevenção & controle
Herpesvirus Equídeo 1/genética
Vacinas contra Herpesvirus/administração & dosagem
Doenças dos Cavalos/virologia
Cavalos
Imunidade Celular
Imunidade Humoral
Imunização
Injeções Intramusculares
Camundongos
Vacinas Atenuadas/administração & dosagem
Vacinas Atenuadas/imunologia
Proteínas do Envelope Viral/imunologia
Vacinas Virais/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Herpesvirus Vaccines); 0 (Vaccines, Attenuated); 0 (Viral Envelope Proteins); 0 (Viral Vaccines); 0 (glycoprotein D, Equid herpesvirus 1)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE


  5 / 1031 MEDLINE  
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[PMID]:28325183
[Au] Autor:Maxwell LK
[Ad] Endereço:Department of Physiological Sciences, Center for Veterinary Health Sciences, Oklahoma State University, 264 McElroy Hall, Stillwater, OK 74078, USA. Electronic address: lk.maxwell@okstate.edu.
[Ti] Título:Antiherpetic Drugs in Equine Medicine.
[So] Source:Vet Clin North Am Equine Pract;33(1):99-125, 2017 Apr.
[Is] ISSN:1558-4224
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Since vaccination may not prevent disease, antiherpetic drugs have been investigated for the therapy of several equine herpesviruses. Drug efficacy has been assessed in horses with disease, but most evidence is in vitro, in other species, or empirical. Oral valacyclovir is most often administered in the therapy of equine herpesvirus type-1 (EHV-1) to protect adult horses from equine herpesvirus myeloencephalopathy, while oral acyclovir is frequently administered for EHV-5 infection in the therapy of equine multinodular pulmonary fibrosis. Other antiherpetic drugs are promising but require further investigation. Several topical drugs are also empirically used in the therapy of equine viral keratitis.
[Mh] Termos MeSH primário: Antivirais/uso terapêutico
Infecções por Herpesviridae/veterinária
Doenças dos Cavalos/tratamento farmacológico
Doenças dos Cavalos/virologia
[Mh] Termos MeSH secundário: Animais
Encefalomielite/tratamento farmacológico
Encefalomielite/veterinária
Encefalomielite/virologia
Infecções por Herpesviridae/tratamento farmacológico
Herpesvirus Equídeo 1/isolamento & purificação
Cavalos
Fibrose Pulmonar/tratamento farmacológico
Fibrose Pulmonar/veterinária
Fibrose Pulmonar/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antiviral Agents)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170602
[Lr] Data última revisão:
170602
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE


  6 / 1031 MEDLINE  
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[PMID]:28241864
[Au] Autor:Zhao J; Poelaert KCK; Van Cleemput J; Nauwynck HJ
[Ad] Endereço:Laboratory of Virology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
[Ti] Título:CCL2 and CCL5 driven attraction of CD172a monocytic cells during an equine herpesvirus type 1 (EHV-1) infection in equine nasal mucosa and the impact of two migration inhibitors, rosiglitazone (RSG) and quinacrine (QC).
[So] Source:Vet Res;48(1):14, 2017 Feb 27.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Equine herpesvirus type 1 (EHV-1) causes respiratory disease, abortion and neurological disorders in horses. Besides epithelial cells, CD172a monocytic cells become infected with EHV-1 in the respiratory mucosa and transport the virus from the apical side of the epithelium to the lamina propria en route to the lymph and blood circulation. Whether CD172a monocytic cells are specifically recruited to the infection sites in order to pick up virus is unknown. In our study, equine nasal mucosa explants were inoculated with EHV-1 neurological strains 03P37 and 95P105 or the non-neurological strains 97P70 and 94P247 and the migration of monocytic cells was examined by immunofluorescence. Further, the role of monokines CCL2 and CCL5 was determined and the effect of migration inhibitors rosiglitazone (RSG) or quinacrine was analyzed. It was shown that with neurological strains but not with the non-neurological strains, CD172a cells specifically migrated towards EHV-1 infected regions and that CCL2 and CCL5 were involved. CCL2 started to be expressed in infected epithelial cells at 24 h post-incubation (hpi) and CCL5 at 48 hpi, which corresponded with the CD172a migration. RSG treatment of EHV-1-inoculated equine nasal mucosa had no effect on the virus replication in the epithelium, but decreased the migration of CD172a cells in the lamina propria. Overall, these findings bring new insights in the early pathogenesis of EHV-1 infections, illustrate differences between neurological and non-neurological strains and show the way for EHV-1 treatment.
[Mh] Termos MeSH primário: Quimiocina CCL2/fisiologia
Quimiocina CCL5/fisiologia
Infecções por Herpesviridae/veterinária
Herpesvirus Equídeo 1
Doenças dos Cavalos/virologia
Monócitos/fisiologia
Mucosa Nasal/virologia
Quinacrina/farmacologia
Receptores Imunológicos/fisiologia
Tiazolidinedionas/farmacologia
[Mh] Termos MeSH secundário: Animais
Movimento Celular/efeitos dos fármacos
Imunofluorescência/veterinária
Infecções por Herpesviridae/imunologia
Infecções por Herpesviridae/virologia
Doenças dos Cavalos/imunologia
Cavalos/virologia
Monócitos/efeitos dos fármacos
Mucosa Nasal/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemokine CCL2); 0 (Chemokine CCL5); 0 (Receptors, Immunologic); 0 (Thiazolidinediones); 05V02F2KDG (rosiglitazone); H0C805XYDE (Quinacrine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1186/s13567-017-0419-4


  7 / 1031 MEDLINE  
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[PMID]:28131380
[Au] Autor:Laval K; Van Cleemput J; Poelaert KC; Brown IK; Nauwynck HJ
[Ad] Endereço:Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
[Ti] Título:Replication of neurovirulent equine herpesvirus type 1 (EHV-1) in CD172a monocytic cells.
[So] Source:Comp Immunol Microbiol Infect Dis;50:58-62, 2017 Feb.
[Is] ISSN:1878-1667
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Equine herpesvirus type 1 (EHV-1) is responsible for respiratory disorders, abortion and myeloencephalopathy (EHM) in horses. Two pathotypes of EHV-1 strains are circulating in the field: neurovirulent (N) and non-neurovirulent (NN). For both strains, CD172a monocytic cells are one of the main carrier cells of EHV-1 during primary infection, allowing the virus to invade the horse's body. Recently, we showed that EHV-1 NN strains showed a restricted and delayed replication in CD172a cells. Here we characterize the in vitro replication kinetics of two EHV-1N strains in CD172a cells and investigate if the replication of these strains is similarly silenced as shown for EHV-1 NN strains. We found that EHV-1N replication was restricted to 7-8% in CD172a cells compared to 100% in control RK-13 cells. EHV-1N replication was not delayed in CD172a cells but virus production was significant lower (10 TCID /10 inoculated cells) than in RK-13 cells (10 TCID /10 inoculated cells). Approximately 0.04% of CD172a cells produced and transmitted infectious EHV-1 to neighbour cells compared to 65% of RK-13 cells. Unlike what we observed for the NN strain, pretreatment of CD172a cells with histone deacetylases inhibitors (HDACi) did not influence the replication of EHV-1N strains in these cells. Overall, these results show that the EHV-1 replication of N strains in CD172a cells differs from that observed for NN strains, which may contribute to their different pathogeneses in vivo.
[Mh] Termos MeSH primário: Herpesvirus Equídeo 1/fisiologia
Monócitos/virologia
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Herpesvirus Equídeo 1/patogenicidade
Cavalos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170217
[Lr] Data última revisão:
170217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170130
[St] Status:MEDLINE


  8 / 1031 MEDLINE  
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[PMID]:28075374
[Au] Autor:Franz M; Goodman LB; Van de Walle GR; Osterrieder N; Greenwood AD
[Ad] Endereço:Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Berlin 10315, Germany. m.franz@izw-berlin.de.
[Ti] Título:A Point Mutation in a Herpesvirus Co-Determines Neuropathogenicity and Viral Shedding.
[So] Source:Viruses;9(1), 2017 Jan 10.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A point mutation in the DNA polymerase gene in equine herpesvirus type 1 (EHV-1) is one determinant for the development of neurological disease in horses. Three recently conducted infection experiments using domestic horses and ponies failed to detect statistically significant differences in viral shedding between the neuropathogenic and non-neuropathogenic variants. These results were interpreted as suggesting the absence of a consistent selective advantage of the neuropathogenic variant and therefore appeared to be inconsistent with a systematic increase in the prevalence of neuropathogenic strains. To overcome potential problems of low statistical power related to small group sizes in these infection experiments, we integrated raw data from all three experiments into a single statistical analysis. The results of this combined analysis showed that infection with the neuropathogenic EHV-1 variant led to a statistically significant increase in viral shedding. This finding is consistent with the idea that neuropathogenic strains could have a selective advantage and are therefore systematically increasing in prevalence in domestic horse populations. However, further studies are required to determine whether a selective advantage indeed exists for neuropathogenic strains.
[Mh] Termos MeSH primário: Herpesvirus Equídeo 1/genética
Herpesvirus Equídeo 1/patogenicidade
Mutação Puntual
Eliminação de Partículas Virais
[Mh] Termos MeSH secundário: Animais
Cavalos
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE


  9 / 1031 MEDLINE  
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[PMID]:28045974
[Au] Autor:Wagner B; Perkins G; Babasyan S; Freer H; Keggan A; Goodman LB; Glaser A; Torsteinsdóttir S; Svansson V; Björnsdóttir S
[Ad] Endereço:Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, United States of America.
[Ti] Título:Neonatal Immunization with a Single IL-4/Antigen Dose Induces Increased Antibody Responses after Challenge Infection with Equine Herpesvirus Type 1 (EHV-1) at Weanling Age.
[So] Source:PLoS One;12(1):e0169072, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Neonatal foals respond poorly to conventional vaccines. These vaccines typically target T-helper (Th) cell dependent B-cell activation. However, Th2-cell immunity is impaired in foals during the first three months of life. In contrast, neonatal basophils are potent interleukin-4 (IL-4) producers. The purpose of this study was to develop a novel vaccine triggering the natural capacity of neonatal basophils to secrete IL-4 and to evaluate if vaccination resulted in B-cell activation and antibody production against EHV-1 glycoprotein C (gC). Neonatal vaccination was performed by oral biotinylated IgE (IgE-bio) treatment at birth followed by intramuscular injection of a single dose of streptavidin-conjugated gC/IL-4 fusion protein (Sav-gC/IL-4) for crosslinking of receptor-bound IgE-bio (group 1). Neonates in group 2 received the intramuscular Sav-gC/IL-4 vaccine only. Group 3 remained non-vaccinated at birth. After vaccination, gC antibody production was not detectable. The ability of the vaccine to induce protection was evaluated by an EHV-1 challenge infection after weaning at 7 months of age. Groups 1 and 2 responded to EHV-1 infection with an earlier onset and overall significantly increased anti-gC serum antibody responses compared to control group 3. In addition, group 1 weanlings had a decreased initial fever peak after infection indicating partial protection from EHV-1 infection. This suggested that the neonatal vaccination induced a memory B-cell response at birth that was recalled at weanling age after EHV-1 challenge. In conclusion, early stimulation of neonatal immunity via the innate arm of the immune system can induce partial protection and increased antibody responses against EHV-1.
[Mh] Termos MeSH primário: Infecções por Herpesviridae/veterinária
Herpesvirus Equídeo 1
Vacinas contra Herpesvirus/uso terapêutico
Doenças dos Cavalos/prevenção & controle
Cavalos/imunologia
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Anticorpos Antivirais/sangue
Formação de Anticorpos
Linfócitos B/imunologia
Linfócitos B/virologia
Citocinas/imunologia
Infecções por Herpesviridae/prevenção & controle
Doenças dos Cavalos/virologia
Interleucina-4/administração & dosagem
Interleucina-4/imunologia
Ativação Linfocitária
Testes de Neutralização/veterinária
Proteínas Recombinantes de Fusão/administração & dosagem
Proteínas Recombinantes de Fusão/imunologia
Temperatura Ambiente
Proteínas do Envelope Viral/administração & dosagem
Proteínas do Envelope Viral/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Cytokines); 0 (Herpesvirus Vaccines); 0 (Recombinant Fusion Proteins); 0 (Viral Envelope Proteins); 0 (glycoprotein C, Equid herpesvirus 1); 207137-56-2 (Interleukin-4)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169072


  10 / 1031 MEDLINE  
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[PMID]:27993615
[Au] Autor:Balasuriya UB; Lee PA; Tsai YL; Tsai CF; Shen YH; Chang HG; Skillman A; Wang HT; Pronost S; Zhang Y
[Ad] Endereço:Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY, USA. Electronic address: ubalasuriya@uky.edu.
[Ti] Título:Translation of a laboratory-validated equine herpesvirus-1 specific real-time PCR assay into an insulated isothermal polymerase chain reaction (iiPCR) assay for point-of-need diagnosis using POCKIT™ nucleic acid analyzer.
[So] Source:J Virol Methods;241:58-63, 2017 Mar.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Equine herpesvirus myeloencephalopathy (EHM), a major problem for the equine industry in the United States, is caused by equine herpesvirus-1 (EHV-1). In addition, EHV-1 is associated with upper respiratory disease, abortion, and chorioretinal lesions in horses. Here we describe the development and evaluation of an inexpensive, user-friendly insulated isothermal PCR (iiPCR) method targeting open reading 30 (ORF30) to detect both neuropathogenic and non-neuropathogenic strains on the field-deployable POCKIT™ device for point-of-need detection of EHV-1. The analytical sensitivity of the EHV-1 iiPCR assay was 13 genome equivalents per reaction. The assay did not cross react with ten non-target equine viral pathogens. Performance of the EHV-1 iiPCR assay was compared to two previously described real-time PCR (qPCR) assays in two laboratories by using 104 archived clinical samples. All 53 qPCR-positive and 46 of the 51 qPCR-negative samples tested positive and negative, respectively, by the iiPCR. The agreement between the two assays was 95.19% (confidence interval 90.48-99.90%) with a kappa value of 0.90. In conclusion, the newly developed EHV-1 iiPCR assay is robust to provide specificity and sensitivity comparable to qPCR assays for the detection of EHV-1 nucleic acid in clinical specimens.
[Mh] Termos MeSH primário: Infecções por Herpesviridae/veterinária
Herpesvirus Equídeo 1/isolamento & purificação
Doenças dos Cavalos/diagnóstico
Reação em Cadeia da Polimerase/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Animais
DNA Viral/genética
DNA Viral/isolamento & purificação
Encefalomielite/diagnóstico
Encefalomielite/veterinária
Encefalomielite/virologia
Infecções por Herpesviridae/diagnóstico
Infecções por Herpesviridae/virologia
Herpesvirus Equídeo 1/genética
Doenças dos Cavalos/virologia
Cavalos
Fases de Leitura Aberta/genética
Reação em Cadeia da Polimerase/economia
Sensibilidade e Especificidade
Temperatura Ambiente
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161221
[St] Status:MEDLINE



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