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  1 / 95 MEDLINE  
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[PMID]:27852816
[Au] Autor:Grau-Roma L; Peckham R; Paton J; Stahel A; de Brot S
[Ad] Endereço:School of Veterinary Medicine and Sciences, University of Nottingham, Sutton Bonington Campus, Loughborough, UK (Grau-Roma, de Brot).
[Ti] Título:Occurrence of primary lymphocytic hypophysitis in two horses and presence of scattered T-lymphocytes in the normal equine pituitary gland.
[So] Source:J Vet Diagn Invest;29(1):115-121, 2017 Jan.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The postmortem examination of a 14-y-old Appaloosa gelding with clinically diagnosed pituitary pars intermedia dysfunction showed a unique finding of moderate multifocal lymphocytic hypophysitis (LH). The pituitary glands of 24 horses submitted for postmortem examination were examined grossly and examined histologically for the presence of lymphocytes. Of these 23 horses, 1 additional case suffered from moderate LH. The 2 cases with LH tested negative for Equid herpesvirus 1 and 4 by polymerase chain reaction and immunohistochemistry (IHC), and no viral particles were observed by electron microscopy in 1 case examined. The cause of LH remains unknown, but based on the T-lymphocytic nature of the inflammation and the human literature, an immune-mediated origin is hypothesized. In addition, the review of 24 cases revealed that 10 horses had few and small multifocal lymphocytic infiltrates within the pituitary gland; the remaining 12 horses showed no evident lymphocytes when examined by hematoxylin and eosin. IHC for CD3 showed the presence of a small number of individual T-lymphocytes scattered through the gland in all examined horses, which appears therefore to be a normal feature of the pituitary gland in horses.
[Mh] Termos MeSH primário: Doenças dos Cavalos/diagnóstico
Hipofisite/veterinária
[Mh] Termos MeSH secundário: Animais
Diagnóstico Diferencial
Herpesvirus Equídeo 1/isolamento & purificação
Herpesvirus Equídeo 4/isolamento & purificação
Doenças dos Cavalos/patologia
Doenças dos Cavalos/virologia
Cavalos
Hipofisite/diagnóstico
Imuno-Histoquímica/veterinária
Masculino
Linfócitos T/patologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161118
[St] Status:MEDLINE
[do] DOI:10.1177/1040638716676292


  2 / 95 MEDLINE  
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[PMID]:27840393
[Au] Autor:Izume S; Kirisawa R; Ohya K; Ohnuma A; Kimura T; Omatsu T; Katayama Y; Mizutani T; Fukushi H
[Ad] Endereço:Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
[Ti] Título:The full genome sequences of 8 equine herpesvirus type 4 isolates from horses in Japan.
[So] Source:J Vet Med Sci;79(1):206-212, 2017 Jan 24.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Equine herpesvirus type 4 (EHV-4) is one of the most important pathogens in horses. To clarify the key genes of the EHV-4 genome that cause abortion in female horses, we determined the whole genome sequences of a laboratory strain and 7 Japanese EHV-4 isolates that were isolated from 2 aborted fetuses and nasal swabs of 5 horses with respiratory disease. The full genome sequences and predicted amino acid sequences of each gene of these isolates were compared with of the reference EHV-4 strain NS80567 and Australian isolates that were reported in 2015. The EHV-4 isolates clustered in 2 groups which did not reflect their pathogenicity. A comparison of the predicted amino acid sequences of the genes did not reveal any genes that were associated with EHV-4-induced abortion.
[Mh] Termos MeSH primário: Genoma Viral/genética
Infecções por Herpesviridae/veterinária
Herpesvirus Equídeo 4/genética
Doenças dos Cavalos/virologia
[Mh] Termos MeSH secundário: Animais
Feminino
Infecções por Herpesviridae/epidemiologia
Infecções por Herpesviridae/virologia
Herpesvirus Equídeo 4/isolamento & purificação
Doenças dos Cavalos/epidemiologia
Cavalos/virologia
Japão/epidemiologia
Análise de Sequência de DNA/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161115
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.16-0506


  3 / 95 MEDLINE  
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[PMID]:28080902
[Au] Autor:Lopez KM; Fleming GJ; Mylniczenko ND
[Ti] Título:A SEROLOGIC AND POLYMERASE CHAIN REACTION SURVEY OF EQUINE HERPESVIRUS IN BURCHELL'S ZEBRAS (EQUUS QUAGGA), HARTMANN'S MOUNTAIN ZEBRAS (EQUUS ZEBRA HARTMANNAE), AND THOMSON'S GAZELLES (EUDORCAS THOMSONII) IN A MIXED SPECIES SAVANNAH EXHIBIT.
[So] Source:J Zoo Wildl Med;47(4):1013-1018, 2016 Dec.
[Is] ISSN:1042-7260
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reports of equine herpesvirus (EHV) 1 and EHV-9 causing clinical disease in a wide range of species have been well documented in the literature. It is thought that zebras are the natural hosts of EHV-9 both in the wild and in captive collections. Concerns about potential interspecies transmission of EHV-1 and EHV-9 in a mixed species savannah exhibit prompted serologic and polymerase chain reaction surveys. Eighteen Burchell's zebras ( Equus quagga ), 11 Hartmann's mountain zebras ( Equus zebra hartmannae), and 14 Thomson's gazelles ( Eudorcas thomsonii ) cohabitating the same exhibit were examined for EHV-1 virus neutralization titers, and evidence of virus via EHV 1-5 polymerase chain reactions. None of the animals had previous exposure to vaccination with EHV-1 or EHV-4. All tested zebras had positive EHV-1 titers, ranging from 4 to 384. All zebras and Thomson's gazelles had negative polymerase chain reaction results for all targeted equine herpesviruses. EHV-9-specific assays are not available but EHV-1, EHV-4, and EHV-9 cross-react serologically. Positive serology results indicate a potential latent equine herpesvirus in the zebra population, which prompted initiation of an equine herpesvirus vaccine protocol, changes in pregnant zebra mare management, and equine herpesvirus polymerase chain reaction screening prior to shipment to or from the study site.
[Mh] Termos MeSH primário: Antílopes
Equidae
Herpesvirus Equídeo 1/isolamento & purificação
Herpesvirus Equídeo 4/isolamento & purificação
Reação em Cadeia da Polimerase/veterinária
Testes Sorológicos/veterinária
[Mh] Termos MeSH secundário: Animais
Animais de Zoológico
Antílopes/virologia
Equidae/virologia
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.1638/2013-0297.1


  4 / 95 MEDLINE  
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[PMID]:27169603
[Au] Autor:Gildea S; Sanchez Higgins MJ; Johnson G; Walsh C; Cullinane A
[Ad] Endereço:Virology Unit, The Irish Equine Centre, Naas, Co. Kildare, Ireland.
[Ti] Título:Concurrent vaccination against equine influenza and equine herpesvirus - a practical approach.
[So] Source:Influenza Other Respir Viruses;10(5):433-7, 2016 09.
[Is] ISSN:1750-2659
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: There is a lack of information concerning concurrent administration of vaccines against equine influenza virus (EIV) and equine herpesvirus 1 and 4 (EHV-1/4). OBJECTIVES: The primary objective of this study was to determine the impact of the concurrent use of EIV and EHV-1/4 vaccines in Thoroughbred racehorses on their humoral immune response to EIV. METHODS: This study was carried out on a population of 30 horses using an inactivated whole-virus EIV vaccine and an inactivated EHV-1/4 vaccine. Horses were randomly allocated to vaccination group A or B. Horses in group A were vaccinated against EIV and EHV-1/4 2 weeks apart. Horses in group B were vaccinated against EIV and EHV-1/4 on the same day. Whole-blood samples were collected on the day of vaccination and 2 weeks and 6 weeks post-vaccination. Antibody levels against EIV and EHV-1/4 were measured using the single radial haemolysis and serum neutralisation test, respectively. RESULTS: The pattern of EIV antibody response post-vaccination was similar for both groups. Highest EIV antibody levels were recorded 2 weeks post-vaccination, and a significant decrease in antibody level was observed 4 weeks later. Horses in group B demonstrated a significantly higher EIV antibody response post-vaccination. Overall, there was no significant difference in EHV-1/4 antibody response between the two groups post-vaccination. CONCLUSION: In this study, concurrent vaccination against EIV and EHV-1/4 increased the response to EIV and did not compromise the humoral immune response to EHV-1/4.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Herpesvirus Equídeo 1/imunologia
Herpesvirus Equídeo 4/imunologia
Vacinas contra Herpesvirus/imunologia
Cavalos/imunologia
Vírus da Influenza A Subtipo H3N8/imunologia
Vacinas contra Influenza/imunologia
[Mh] Termos MeSH secundário: Resinas Acrílicas
Adjuvantes Imunológicos
Animais
Infecções por Herpesviridae/imunologia
Infecções por Herpesviridae/prevenção & controle
Infecções por Herpesviridae/virologia
Herpesvirus Equídeo 1/isolamento & purificação
Herpesvirus Equídeo 4/isolamento & purificação
Vacinas contra Herpesvirus/administração & dosagem
Doenças dos Cavalos/epidemiologia
Doenças dos Cavalos/prevenção & controle
Doenças dos Cavalos/virologia
Imunidade Humoral
Vírus da Influenza A Subtipo H3N8/isolamento & purificação
Vírus da Influenza A/imunologia
Vacinas contra Influenza/administração & dosagem
Infecções por Orthomyxoviridae/imunologia
Infecções por Orthomyxoviridae/prevenção & controle
Infecções por Orthomyxoviridae/veterinária
Infecções por Orthomyxoviridae/virologia
Distribuição Aleatória
Vacinação/métodos
Vacinação/veterinária
Vacinas de Produtos Inativados/administração & dosagem
Vacinas de Produtos Inativados/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acrylic Resins); 0 (Adjuvants, Immunologic); 0 (Antibodies, Viral); 0 (Herpesvirus Vaccines); 0 (Influenza Vaccines); 0 (Vaccines, Inactivated); 0A5MM307FC (carboxypolymethylene)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160513
[St] Status:MEDLINE
[do] DOI:10.1111/irv.12396


  5 / 95 MEDLINE  
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[PMID]:26984900
[Au] Autor:Cruz F; Fores P; Mughini-Gras L; Ireland J; Moreno MA; Newton JR
[Ad] Endereço:VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain.
[Ti] Título:Seroprevalence and factors associated with equine herpesvirus type 1 and 4 in Spanish Purebred horses in Spain.
[So] Source:Vet Rec;178(16):398, 2016 Apr 16.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Equine herpesvirus type 1 (EHV-1) and type 4 (EHV-4) have a worldwide distribution and cause respiratory disease, abortion, neonatal death and myeloencephalopathy in susceptible horses. Given the scarcity of serological EHV-1/EHV-4 data in Spain, the objective of this cross-sectional study was to estimate the seroprevalence of EHV-1/EHV-4 and to identify potential horse-level and stud farm-level factors associated with EHV-1/EHV-4 in the breeding Spanish Purebred (SP) horse population in central Spain. Serum samples from 334 SP unvaccinated horses, collected between September 2011 and November 2013 at 30 stud farms, were tested using a commercially available EHV-1/EHV-4 antibody ELISA and seroneutralisation as the World Organisation for Animal Health reference confirmation test. Data on factors putatively associated with seropositivity to EHV-1/EHV-4 were collected via a questionnaire and examined using logistic regression analysis. EHV-1/EHV-4 seroprevalence in the SP breeding population in central Spain, standardised for the sex distribution of the reference horse population, was 53.9 per cent (95 per cent confidence interval 44.0 per cent to 63.8 per cent). Increasing age, southern location of the stud farm, temperate climate during the summer, and a smaller surface area used for breeding activities in the farm were associated with increased odds for EHV-1/EHV-4 seropositivity, whereas EHV-1/EHV-4 vaccination of other resident horses and separation of breeding mares from youngsters were protective factors.
[Mh] Termos MeSH primário: Infecções por Herpesviridae/veterinária
Herpesvirus Equídeo 1/isolamento & purificação
Herpesvirus Equídeo 4/isolamento & purificação
Doenças dos Cavalos/epidemiologia
Doenças dos Cavalos/virologia
[Mh] Termos MeSH secundário: Animais
Cruzamento
Estudos Transversais
Ensaio de Imunoadsorção Enzimática/veterinária
Feminino
Infecções por Herpesviridae/epidemiologia
Infecções por Herpesviridae/virologia
Cavalos
Masculino
Gravidez
Fatores de Risco
Estudos Soroepidemiológicos
Espanha/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160416
[Lr] Data última revisão:
160416
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160318
[St] Status:MEDLINE
[do] DOI:10.1136/vr.103573


  6 / 95 MEDLINE  
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[PMID]:26691326
[Au] Autor:Vaz PK; Horsington J; Hartley CA; Browning GF; Ficorilli NP; Studdert MJ; Gilkerson JR; Devlin JM
[Ad] Endereço:1​ Asia-Pacific Centre for Animal Health, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia.
[Ti] Título:Evidence of widespread natural recombination among field isolates of equine herpesvirus 4 but not among field isolates of equine herpesvirus 1.
[So] Source:J Gen Virol;97(3):747-55, 2016 Mar.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recombination in alphaherpesviruses allows evolution to occur in viruses that have an otherwise stable DNA genome with a low rate of nucleotide substitution. High-throughput sequencing of complete viral genomes has recently allowed natural (field) recombination to be studied in a number of different alphaherpesviruses, however, such studies have not been applied to equine herpesvirus 1 (EHV-1) or equine herpesvirus 4 (EHV-4). These two equine alphaherpesviruses are genetically similar, but differ in their pathogenesis and epidemiology. Both cause economically significant disease in horse populations worldwide. This study used high-throughput sequencing to determine the full genome sequences of EHV-1 and EHV-4 isolates (11 and 14 isolates, respectively) from Australian or New Zealand horses. These sequences were then analysed and examined for evidence of recombination. Evidence of widespread recombination was detected in the genomes of the EHV-4 isolates. Only one potential recombination event was detected in the genomes of the EHV-1 isolates, even when the genomes from an additional 11 international EHV-1 isolates were analysed. The results from this study reveal another fundamental difference between the biology of EHV-1 and EHV-4. The results may also be used to help inform the future safe use of attenuated equine herpesvirus vaccines.
[Mh] Termos MeSH primário: Infecções por Herpesviridae/veterinária
Herpesvirus Equídeo 1/genética
Herpesvirus Equídeo 4/genética
Doenças dos Cavalos/virologia
Recombinação Genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Genoma Viral
Infecções por Herpesviridae/virologia
Herpesvirus Equídeo 1/classificação
Herpesvirus Equídeo 1/isolamento & purificação
Herpesvirus Equídeo 4/classificação
Herpesvirus Equídeo 4/isolamento & purificação
Cavalos
Dados de Sequência Molecular
Nova Zelândia
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151223
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000378


  7 / 95 MEDLINE  
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[PMID]:26424485
[Au] Autor:Bannai H; Nemoto M; Tsujimura K; Yamanaka T; Maeda K; Kondo T
[Ad] Endereço:Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
[Ti] Título:Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen.
[So] Source:J Vet Med Sci;78(2):309-11, 2016 Feb.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.
[Mh] Termos MeSH primário: Antígenos Virais/imunologia
Ensaio de Imunoadsorção Enzimática/veterinária
Herpesvirus Equídeo 4/isolamento & purificação
Doenças dos Cavalos/virologia
Proteínas do Envelope Viral/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Técnicas de Química Sintética
Ensaio de Imunoadsorção Enzimática/métodos
Cavalos
Fragmentos de Peptídeos/síntese química
Fragmentos de Peptídeos/imunologia
Sensibilidade e Especificidade
Proteínas do Envelope Viral/síntese química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Viral); 0 (Peptide Fragments); 0 (Viral Envelope Proteins); 148349-55-7 (glycoprotein G, Equid herpesvirus 4)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151002
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.15-0275


  8 / 95 MEDLINE  
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[PMID]:26489864
[Au] Autor:Azab W; Gramatica A; Herrmann A; Osterrieder N
[Ad] Endereço:Institut für Virologie, Robert von Ostertag-Haus, Zentrum für Infektionsmedizin, Freie Universität Berlin, Berlin, Germany wfazab@zedat.fu-berlin.de.
[Ti] Título:Binding of alphaherpesvirus glycoprotein H to surface α4ß1-integrins activates calcium-signaling pathways and induces phosphatidylserine exposure on the plasma membrane.
[So] Source:MBio;6(5):e01552-15, 2015 Oct 20.
[Is] ISSN:2150-7511
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Intracellular signaling connected to integrin activation is known to induce cytoplasmic Ca(2+) release, which in turn mediates a number of downstream signals. The cellular entry pathways of two closely related alphaherpesviruses, equine herpesviruses 1 and 4 (EHV-1 and EHV-4), are differentially regulated with respect to the requirement of interaction of glycoprotein H (gH) with α4ß1-integrins. We show here that binding of EHV-1, but not EHV-4, to target cells resulted in a rapid and significant increase in cytosolic Ca(2+) levels. EHV-1 expressing EHV-4 gH (gH4) in lieu of authentic gH1 failed to induce Ca(2+) release, while EHV-4 with gH1 triggered significant Ca(2+) release. Blocking the interaction between gH1 and α4ß1-integrins, inhibiting phospholipase C (PLC) activation, or blocking binding of inositol 1,4,5-triphosphate (IP3) to its receptor on the endoplasmic reticulum (ER) abrogated Ca(2+) release. Interestingly, phosphatidylserine (PS) was exposed on the plasma membrane in response to cytosolic calcium increase after EHV-1 binding through a scramblase-dependent mechanism. Inhibition of both Ca(2+) release from the ER and scramblase activation blocked PS scrambling and redirected virus entry to the endocytic pathway, indicating that PS may play a role in facilitating virus entry directly at the plasma membrane. IMPORTANCE: Herpesviruses are a large family of enveloped viruses that infect a wide range of hosts, causing a variety of diseases. These viruses have developed a number of strategies for successful entry into different cell types. We and others have shown that alphaherpesviruses, including EHV-1 and herpes simplex virus 1 (HSV-1), can route their entry pathway and do so by manipulation of cell signaling cascades to ensure viral genome delivery to nuclei. We show here that the interaction between EHV-1 gH and cellular α4ß1-integrins is necessary to induce emptying of ER calcium stores, which induces phosphatidylserine exposure on the plasma membrane through a scramblase-dependent mechanism. This change in lipid asymmetry facilitates virus entry and might help fusion of the viral envelope at the plasma membrane. These findings will help to advance our understanding of herpesvirus entry mechanism and may facilitate the development of novel drugs that can be implemented for prevention of infection and disease.
[Mh] Termos MeSH primário: Sinalização do Cálcio
Membrana Celular/química
Herpesvirus Equídeo 1/fisiologia
Herpesvirus Equídeo 4/fisiologia
Integrina alfa4beta1/metabolismo
Fosfatidilserinas/análise
Proteínas Estruturais Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Cavalos
Ligação Proteica
Internalização do Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Integrin alpha4beta1); 0 (Phosphatidylserines); 0 (Viral Structural Proteins)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151023
[St] Status:MEDLINE


  9 / 95 MEDLINE  
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[PMID]:26421307
[Au] Autor:Taktaz Hafshejani T; Nekoei S; Vazirian B; Doosti A; Khamesipour F; Anyanwu MU
[Ad] Endereço:Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
[Ti] Título:Molecular Detection of Equine Herpesvirus Types 1 and 4 Infection in Healthy Horses in Isfahan Central and Shahrekord Southwest Regions, Iran.
[So] Source:Biomed Res Int;2015:917854, 2015.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study was undertaken to investigate molecularly the occurrence of EHV-1 and EHV-4 infection among equine population in regions, Iran. Blood samples from 53 and 37 randomly selected horses settled in Isfahan and Shahrekord, Iran, respectively, were collected. Detection of EHV-1 and EHV-4 genes in the blood samples was done using polymerase chain reaction (PCR). Out of 53 and 37 samples from Isfahan and Shahrekord, 4 (18.18%) and 3 (8.10%) were positive for PCR of EHV-1, respectively. Nine (16.98%) and 6 (16.21%) were positive for PCR of EHV-4, while 6 (11.32%) and 3 (8.10%) were positive for PCR of both EHV-1 and EHV-4, in Isfahan and Shahrekord, respectively. Of the 7 blood samples positive for EHV-1, 4 (16.66%) and 3 (8.10%) were from horses >3 years old while 2 (18.18%) and 1 (16.66%) were from 2-3 years old horses, in Isfahan and Shahrekord, respectively. Out of the 7 and 3 samples positive for PCR of EHV-1 in Isfahan and Shahrekord, 4 (22.2%) and 1 (7.69%) were Standardbred, while 3 (14.28%) and 2 (13.33%) were Thoroughbreds, respectively. EHV-4 was detected in blood of 4 (22.22%) and 2 (15.83%) Standardbreds and from 4 (19.04%) and 4 (26.66%) Thoroughbred horses in Isfahan and Shahrekord, respectively. This study has shown that horses settled in Isfahan central and Shahrekord southwest regions, Iran, are infected by EHV-1 and EHV-4 and thus serve as potential reservoirs and disseminators of the viruses.
[Mh] Termos MeSH primário: Infecções por Herpesviridae/veterinária
Herpesvirus Equídeo 1/genética
Herpesvirus Equídeo 4/genética
Doenças dos Cavalos/virologia
Cavalos/virologia
[Mh] Termos MeSH secundário: Envelhecimento
Animais
Cruzamento
Eletroforese em Gel de Ágar
Genes Virais
Infecções por Herpesviridae/virologia
Irã (Geográfico)
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151001
[St] Status:MEDLINE
[do] DOI:10.1155/2015/917854


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[PMID]:26378176
[Au] Autor:Spiesschaert B; Goldenbogen B; Taferner S; Schade M; Mahmoud M; Klipp E; Osterrieder N; Azab W
[Ad] Endereço:Institut für Virologie, Robert von Ostertag-Haus, Zentrum für Infektionsmedizin, Freie Universität Berlin, Berlin, Germany.
[Ti] Título:Role of gB and pUS3 in Equine Herpesvirus 1 Transfer between Peripheral Blood Mononuclear Cells and Endothelial Cells: a Dynamic In Vitro Model.
[So] Source:J Virol;89(23):11899-908, 2015 Dec.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Infected peripheral blood mononuclear cells (PBMC) effectively transport equine herpesvirus type 1 (EHV-1), but not EHV-4, to endothelial cells (EC) lining the blood vessels of the pregnant uterus or central nervous system, a process that can result in abortion or myeloencephalopathy. We examined, using a dynamic in vitro model, the differences between EHV-1 and EHV-4 infection of PBMC and PBMC-EC interactions. In order to evaluate viral transfer between infected PBMC and EC, cocultivation assays were performed. Only EHV-1 was transferred from PBMC to EC, and viral glycoprotein B (gB) was shown to be mainly responsible for this form of cell-to-cell transfer. For addressing the more dynamic aspects of PBMC-EC interaction, infected PBMC were perfused through a flow channel containing EC in the presence of neutralizing antibodies. By simulating capillary blood flow and analyzing the behavior of infected PBMC through live fluorescence imaging and automated cell tracking, we observed that EHV-1 was able to maintain tethering and rolling of infected PBMC on EC more effectively than EHV-4. Deletion of US3 reduced the ability of infected PBMC to tether and roll compared to that of cells infected with parental virus, which resulted in a significant reduction in virus transfer from PBMC to EC. Taking the results together, we conclude that systemic spread and EC infection by EHV-1, but not EHV-4, is caused by its ability to infect and/or reprogram mononuclear cells with respect to their tethering and rolling behavior on EC and consequent virus transfer. IMPORTANCE: EHV-1 is widespread throughout the world and causes substantial economic losses through outbreaks of respiratory disease, abortion, and myeloencephalopathy. Despite many years of research, no fully protective vaccines have been developed, and several aspects of viral pathogenesis still need to be uncovered. In the current study, we investigated the molecular mechanisms that facilitate the cell-associated viremia, which is arguably the most important aspect of EHV-1 pathogenesis. The newly discovered functions of gB and pUS3 add new facets to their previously reported roles. Due to the conserved nature of cell-associated viremia among numerous herpesviruses, these results are also very relevant for viruses such as varicella-zoster virus, pseudorabies virus, human cytomegalovirus, and others. In addition, the constructed mutant and recombinant viruses exhibit potent in vitro replication but have significant defects in certain stages of the disease course. These viruses therefore show much promise as candidates for future live vaccines.
[Mh] Termos MeSH primário: Células Endoteliais/virologia
Infecções por Herpesviridae/fisiopatologia
Herpesvirus Equídeo 1/fisiologia
Herpesvirus Equídeo 4/fisiologia
Leucócitos Mononucleares/virologia
Proteínas Serina-Treonina Quinases/metabolismo
Proteínas do Envelope Viral/metabolismo
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Agregação Celular
Células Cultivadas
Fluorescência
Cavalos
Técnicas In Vitro
Estatísticas não Paramétricas
Internalização do Vírus
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Envelope Proteins); 0 (glycoprotein B, Simplexvirus); EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:160601
[Lr] Data última revisão:
160601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150918
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.01809-15



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