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  1 / 2487 MEDLINE  
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[PMID]:28467177
[Au] Autor:Sayler KA; Bigelow T; Koster LG; Swenson S; Bounds C; Hernández F; Wisely SM
[Ad] Endereço:Department of Wildlife Ecology and Conservation, University of Florida, Gainesville, FL (Sayler, Bounds, Hernández, Wisely).
[Ti] Título:Development of a rapid, simple, and specific real-time PCR assay for detection of pseudorabies viral DNA in domestic swine herds.
[So] Source:J Vet Diagn Invest;29(4):522-528, 2017 Jul.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite successful eradication of pseudorabies virus (PRV) from the commercial pig industry in the United States in 2004, large populations of feral swine in certain regions act as wildlife reservoirs for the virus. Given the threat of reintroduction of the virus into domestic herds, a rapid, reliable, easily implemented assay is needed for detection of PRV. Although a real-time PCR (rtPCR) assay exists, improvements in rtPCR technology and a greater understanding of the diversity of PRV strains worldwide require an assay that would be easier to implement, more cost effective, and more specific. We developed a single-tube, rapid rtPCR that is capable of detecting 10 copies of PRV glycoprotein B ( gB) DNA per 20-µL total volume reaction. The assay did not produce a false-positive in samples known to be negative for the virus. The assay was negative for genetically similar herpesviruses and other porcine viruses. Our assay is a highly specific and sensitive assay that is also highly repeatable and reproducible. The assay should be a useful tool for early detection of PRV in pigs in the case of a suspected introduction or outbreak situation.
[Mh] Termos MeSH primário: DNA Viral/análise
Herpesvirus Suídeo 1/isolamento & purificação
Pseudorraiva/diagnóstico
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Doenças dos Suínos/diagnóstico
Proteínas do Envelope Viral/análise
[Mh] Termos MeSH secundário: Animais
Pseudorraiva/virologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
Sensibilidade e Especificidade
Sus scrofa
Suínos
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Envelope Proteins); 0 (glycoprotein gII, pseudorabies virus)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171212
[Lr] Data última revisão:
171212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1177/1040638717706593


  2 / 2487 MEDLINE  
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[PMID]:29073268
[Au] Autor:Koyuncu OO; MacGibeny MA; Hogue IB; Enquist LW
[Ad] Endereço:Department of Molecular Biology, and Princeton Neuroscience Institute, Princeton University, Princeton, NJ, United States of America.
[Ti] Título:Compartmented neuronal cultures reveal two distinct mechanisms for alpha herpesvirus escape from genome silencing.
[So] Source:PLoS Pathog;13(10):e1006608, 2017 Oct.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Alpha herpesvirus genomes encode the capacity to establish quiescent infections (i.e. latency) in the peripheral nervous system for the life of their hosts. Multiple times during latency, viral genomes can reactivate to start a productive infection, enabling spread of progeny virions to other hosts. Replication of alpha herpesviruses is well studied in cultured cells and many aspects of productive replication have been identified. However, many questions remain concerning how a productive or a quiescent infection is established. While infections in vivo often result in latency, infections of dissociated neuronal cultures in vitro result in a productive infection unless lytic viral replication is suppressed by DNA polymerase inhibitors or interferon. Using primary peripheral nervous system neurons cultured in modified Campenot tri-chambers, we previously reported that reactivateable, quiescent infections by pseudorabies virus (PRV) can be established in the absence of any inhibitor. Such infections were established in cell bodies only when physically isolated axons were infected at a very low multiplicity of infection (MOI). In this report, we developed a complementation assay in compartmented neuronal cultures to investigate host and viral factors in cell bodies that prevent establishment of quiescent infection and promote productive replication of axonally delivered genomes (i.e. escape from silencing). Stimulating protein kinase A (PKA) signaling pathways in isolated cell bodies, or superinfecting cell bodies with either UV-inactivated PRV or viral light particles (LP) promoted escape from genome silencing and prevented establishment of quiescent infection but with different molecular mechanisms. Activation of PKA in cell bodies triggers a slow escape from silencing in a cJun N-terminal kinase (JNK) dependent manner. However, escape from silencing is induced rapidly by infection with UVPRV or LP in a PKA- and JNK-independent manner. We suggest that viral tegument proteins delivered to cell bodies engage multiple signaling pathways that block silencing of viral genomes delivered by low MOI axonal infection.
[Mh] Termos MeSH primário: Regulação Viral da Expressão Gênica/genética
Inativação Gênica
Herpesvirus Humano 1/genética
Herpesvirus Suídeo 1/genética
Neurônios/virologia
Replicação Viral/genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Genoma Viral/genética
Herpesvirus Humano 1/fisiologia
Suínos
Proteínas Virais/genética
Latência Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006608


  3 / 2487 MEDLINE  
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[PMID]:29035172
[Au] Autor:Liu YT; Jiang J; Bohannon KP; Dai X; Gant Luxton GW; Hui WH; Bi GQ; Smith GA; Hong Zhou Z
[Ad] Endereço:2​California NanoSystems Institute, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA.
[Ti] Título:A pUL25 dimer interfaces the pseudorabies virus capsid and tegument.
[So] Source:J Gen Virol;98(11):2837-2849, 2017 Nov.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Inside the virions of α-herpesviruses, tegument protein pUL25 anchors the tegument to capsid vertices through direct interactions with tegument proteins pUL17 and pUL36. In addition to promoting virion assembly, both pUL25 and pUL36 are critical for intracellular microtubule-dependent capsid transport. Despite these essential roles during infection, the stoichiometry and precise organization of pUL25 and pUL36 on the capsid surface remain controversial due to the insufficient resolution of existing reconstructions from cryo-electron microscopy (cryoEM). Here, we report a three-dimensional (3D) icosahedral reconstruction of pseudorabies virus (PRV), a varicellovirus of the α-herpesvirinae subfamily, obtained by electron-counting cryoEM at 4.9 Å resolution. Our reconstruction resolves a dimer of pUL25 forming a capsid-associated tegument complex with pUL36 and pUL17 through a coiled coil helix bundle, thus correcting previous misinterpretations. A comparison between reconstructions of PRV and the γ-herpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV) reinforces their similar architectures and establishes important subfamily differences in the capsid-tegument interface.
[Mh] Termos MeSH primário: Herpesvirus Suídeo 1/química
Herpesvirus Suídeo 1/ultraestrutura
Multimerização Proteica
Proteínas Estruturais Virais/análise
Proteínas Estruturais Virais/ultraestrutura
Vírion/química
Vírion/ultraestrutura
[Mh] Termos MeSH secundário: Microscopia Crioeletrônica
Imagem Tridimensional
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Structural Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171114
[Lr] Data última revisão:
171114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000903


  4 / 2487 MEDLINE  
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[PMID]:28900740
[Au] Autor:Tang YD; Liu JT; Wang TY; Sun MX; Tian ZJ; Cai XH
[Ad] Endereço:State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 678 HaPing Road, XiangFang, Harbin, 150069, China.
[Ti] Título:CRISPR/Cas9-mediated multiple single guide RNAs potently abrogate pseudorabies virus replication.
[So] Source:Arch Virol;162(12):3881-3886, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Pseudorabies virus (PRV) is a swine herpesvirus that causes significant morbidity and mortality in swine populations and has caused huge economic losses in the worldwide swine industry. Currently, there is no effective antiviral drug in clinical use for PRV infection; it is also difficult to eliminate PRV from infected swine. In our study, we set out to combat these swine herpesvirus infections by exploiting the CRISPR/Cas9 system. We designed 75 single guide RNAs (sgRNA) by targeting both essential and non-essential genes across the genome of PRV. We applied a firefly luciferase-tagged reporter PRV virus for high-throughput sgRNA screening and found that most of the sgRNAs significantly inhibited PRV replication. More importantly, using a transfection assay, we demonstrated that simultaneous targeting of PRV with multiple sgRNAs completely abolished the production of infectious viruses in cells. These data suggest that CRISPR/Cas9 could be a novel therapeutic agent against PRV in the future.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Produtos Biológicos/farmacologia
Herpesvirus Suídeo 1/efeitos dos fármacos
Herpesvirus Suídeo 1/fisiologia
RNA Guia/farmacologia
Replicação Viral/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antivirais/isolamento & purificação
Produtos Biológicos/isolamento & purificação
Sistemas CRISPR-Cas
Linhagem Celular
Marcação de Genes
RNA Guia/isolamento & purificação
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Biological Products); 0 (RNA, Guide)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3553-4


  5 / 2487 MEDLINE  
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[PMID]:28631596
[Au] Autor:Wang J; Zeng L; Zhang L; Guo ZZ; Lu SF; Ming SL; Li GL; Wan B; Tian KG; Yang GY; Chu BB
[Ad] Endereço:1​College of Animal Sciences and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, Henan Province, PR China.
[Ti] Título:Cholesterol 25-hydroxylase acts as a host restriction factor on pseudorabies virus replication.
[So] Source:J Gen Virol;98(6):1467-1476, 2017 Jun.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cholesterol 25-hydroxylase (CH25H) catalyses the production of 25-hydroxycholesterol (25HC) from cholesterol by adding a second hydroxyl group at position 25. The aim of this study was to examine the antiviral effect of CH25H on pseudorabies virus (PRV), a swine pathogen that can cause devastating disease and economic losses worldwide. The results showed that porcine ch25h was induced by either interferon or PRV infection. PRV infection of porcine alveolar macrophages (3D4/21 cells) was attenuated by CH25H overexpression and enhanced by silencing of CH25H. Furthermore, treatment of 3D4/21 cells with 25HC inhibited the growth of PRV in vitro, suggesting that CH25H may restrict PRV replication by 25HC production. We further identified that the anti-PRV role of CH25H and 25HC was subject to their inhibitory effect on PRV attachment and entry. Collectively, these findings demonstrate that CH25H is an intrinsic host restriction factor in PRV infection of porcine alveolar macrophages.
[Mh] Termos MeSH primário: Antivirais/metabolismo
Herpesvirus Suídeo 1/crescimento & desenvolvimento
Herpesvirus Suídeo 1/imunologia
Interações Hospedeiro-Patógeno
Hidroxicolesteróis/metabolismo
Esteroide Hidroxilases/metabolismo
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Imunidade Inata
Macrófagos Alveolares/imunologia
Macrófagos Alveolares/virologia
Suínos
Ligação Viral/efeitos dos fármacos
Internalização do Vírus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Hydroxycholesterols); 767JTD2N31 (25-hydroxycholesterol); EC 1.14.- (Steroid Hydroxylases); EC 1.14.99.38 (cholesterol 25-hydroxylase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000797


  6 / 2487 MEDLINE  
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[PMID]:28363130
[Au] Autor:Wang X; Wu CX; Song XR; Chen HC; Liu ZF
[Ad] Endereço:State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
[Ti] Título:Comparison of pseudorabies virus China reference strain with emerging variants reveals independent virus evolution within specific geographic regions.
[So] Source:Virology;506:92-98, 2017 Jun.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pseudorabies virus (PRV) China reference strain Ea is genetically closely related to newly emerged variants; however, there is limited information about PRV Ea. Here, we compared PRV Ea with new variant strains by growth kinetics, genome sequencing, and protein expression analysis. Growth analysis showed that strain Ea forms smaller plaques than strain HNX. The full-length genome sequence of Ea revealed that it is clustered in the same subgroup as HNX. Ea and HNX strains exhibited similar extracellular virion protein polymorphisms, whereas strain Bartha expressed less VP26 and more GAPDH. In infected cells, strain Ea expressed high levels of IE180 protein, and Ea and HNX produced higher levels of UL21 protein than strain Bartha. These findings provide evidence that PRV China reference strain Ea is genetically closely related to the newly emerged variant strains, indicating that strain PRV China may have evolved independently leading to the emergence of a variant strain.
[Mh] Termos MeSH primário: Evolução Molecular
Herpesvirus Suídeo 1/genética
Herpesvirus Suídeo 1/isolamento & purificação
Pseudorraiva/virologia
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
China
Herpesvirus Suídeo 1/classificação
Herpesvirus Suídeo 1/metabolismo
Filogenia
Suínos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE


  7 / 2487 MEDLINE  
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[PMID]:28228592
[Au] Autor:Vallbracht M; Rehwaldt S; Klupp BG; Mettenleiter TC; Fuchs W
[Ad] Endereço:Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.
[Ti] Título:Functional Relevance of the N-Terminal Domain of Pseudorabies Virus Envelope Glycoprotein H and Its Interaction with Glycoprotein L.
[So] Source:J Virol;91(9), 2017 May 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Several envelope glycoproteins are involved in herpesvirus entry into cells, direct cell-to-cell spread, and induction of cell fusion. The membrane fusion protein glycoprotein B (gB) and the presumably gB-activating heterodimer gH/gL are essential for these processes and conserved throughout the However, after extended cell culture passage of gL-negative mutants of the alphaherpesvirus pseudorabies virus (PrV), phenotypic revertants could be isolated which had acquired spontaneous mutations affecting the gL-interacting N-terminal part of the gH ectodomain (gDH and gH ) (B. G. Klupp and T. C. Mettenleiter, J Virol 73:3014-3022, 1999; C. Schröter, M. Vallbracht, J. Altenschmidt, S. Kargoll, W. Fuchs, B. G. Klupp, and T. C. Mettenleiter, J Virol 90:2264-2272, 2016). To investigate the functional relevance of this part of gH in more detail, we introduced an in-frame deletion of 66 codons at the 5' end of the plasmid-cloned gH gene (gH ). The N-terminal signal peptide was retained, and the deletion did not affect expression or processing of gH but abrogated its function in fusion assays. Insertion of the engineered gH gene into the PrV genome resulted in a defective mutant (pPrV-gH K), which was incapable of entry and spread. Interestingly, activity of mutated gH was restored when it was coexpressed with hyperfusogenic gB , obtained from a passaged gL deletion mutant of PrV. Moreover, the entry and spread defects of pPrV-gH K were compensated by the mutations in gB in , as well as in , independent of gL. Thus, PrV gL and the gL-interacting domain of gH are not strictly required for function. Membrane fusion is crucial for infectious entry and spread of enveloped viruses. While many enveloped viruses require only one or two proteins for receptor binding and membrane fusion, herpesvirus infection depends on several envelope glycoproteins. Besides subfamily-specific receptor binding proteins, the core fusion machinery consists of the conserved fusion protein gB and the gH/gL complex. The role of the latter is unclear, but it is hypothesized to interact with gB for fusion activation. Using isogenic virus recombinants, we demonstrate here that gL and the gL-binding domain of PrV gH are not strictly required for membrane fusion during virus entry and spread when concomitantly mutations in gB are present which increase its fusogenicity. Thus, our results strongly support the notion of a functional gB-gH interaction during the fusion process.
[Mh] Termos MeSH primário: Herpesvirus Suídeo 1/genética
Fusão de Membrana/genética
Proteínas do Envelope Viral/genética
Proteínas do Envelope Viral/metabolismo
Ligação Viral
Internalização do Vírus
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Herpesvirus Suídeo 1/metabolismo
Ligação Proteica/genética
Estrutura Terciária de Proteína/genética
Coelhos
Deleção de Sequência/genética
Replicação Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Envelope Proteins); 138361-42-9 (glycoprotein gH, pseudorabies virus)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE


  8 / 2487 MEDLINE  
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[PMID]:28224417
[Au] Autor:Zhou YT; He ZG; Liu TT; Feng MH; Zhang DY; Xiang HB
[Ad] Endereço:Department of Surgery, Shuyang Hospital, Shuyang, 223600, China.
[Ti] Título:Neuroanatomical circuitry between kidney and rostral elements of brain: a virally mediated transsynaptic tracing study in mice.
[So] Source:J Huazhong Univ Sci Technolog Med Sci;37(1):63-69, 2017 Feb.
[Is] ISSN:1672-0733
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:The identity of higher-order neurons and circuits playing an associative role to control renal function is not well understood. We identified specific neural populations of rostral elements of brain regions that project multisynaptically to the kidneys in 3-6 days after injecting a retrograde tracer pseudorabies virus (PRV)-614 into kidney of 13 adult male C57BL/6J strain mice. PRV-614 infected neurons were detected in a number of mesencephalic (e.g. central amygdala nucleus), telencephalic regions and motor cortex. These divisions included the preoptic area (POA), dorsomedial hypothalamus (DMH), lateral hypothalamus, arcuate nucleus (Arc), suprachiasmatic nucleus (SCN), periventricular hypothalamus (PeH), and rostral and caudal subdivision of the paraventricular nucleus of the hypothalamus (PVN). PRV-614/Tyrosine hydroxylase (TH) double-labeled cells were found within DMH, Arc, SCN, PeH, PVN, the anterodorsal and medial POA. A subset of neurons in PVN that participated in regulating sympathetic outflow to kidney was catecholaminergic or serotonergic. PRV-614 infected neurons within the PVN also contained arginine vasopressin or oxytocin. These data demonstrate the rostral elements of brain innervate the kidney by the neuroanatomical circuitry.
[Mh] Termos MeSH primário: Encéfalo/virologia
Herpesvirus Suídeo 1/fisiologia
Rim/inervação
Vias Neurais
[Mh] Termos MeSH secundário: Animais
Encéfalo/enzimologia
Masculino
Mesencéfalo/enzimologia
Mesencéfalo/virologia
Camundongos
Camundongos Endogâmicos C57BL
Vias Neurais/anatomia & histologia
Vias Neurais/virologia
Núcleo Hipotalâmico Paraventricular/enzimologia
Núcleo Hipotalâmico Paraventricular/virologia
Telencéfalo/enzimologia
Telencéfalo/virologia
Tirosina 3-Mono-Oxigenase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1007/s11596-017-1695-y


  9 / 2487 MEDLINE  
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[PMID]:28215695
[Au] Autor:Huangfu C; Ma Y; Jia J; Lv M; Zhu F; Ma X; Zhao X; Zhang J
[Ad] Endereço:Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, 100850, China.
[Ti] Título:Inactivation of viruses by pasteurization at 60 °C for 10 h with and without 40% glucose as stabilizer during a new manufacturing process of α2-Macroglobulin from Cohn Fraction IV.
[So] Source:Biologicals;46:139-142, 2017 Mar.
[Is] ISSN:1095-8320
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pasteurization is regularly used to inactivate viruses for the safety of plasma derivatives. Influence of pasteurization at 60 °C for 10 h on α2-Macroglobulin activity and virus inactivation were studied. With 40% sugar as stabilizers more than 70% α2-Macroglobulin activity was reserved after pasteurization compared with 20% in control. Glucose presented a better activity protection effect than sucrose and maltose. By pasteurization without stabilizer the virus titers of pseudorabies virus, Sindbis virus, porcine parvovirus and encephalomyocarditis virus were reduced more than 5.88 log , 7.50 log , 4.88 log , and 5.63 log respectively within 2 h. By pasteurization with 40% glucose vesicular stomatitis virus was inactivated more than 5.88 log within 1 h. Only 2.71 log reduction was achieved for encephalomyocarditis virus after 10 h. 40% glucose protected α2-M activity and viruses simultaneously from pasteurization. Other viral inactivation methods need to be incorporated to ensure viral safety of this manufacturing process of α2-Macroglobulin.
[Mh] Termos MeSH primário: Proteínas Sanguíneas/metabolismo
Glucose/farmacologia
Temperatura Alta
Pasteurização/métodos
Inativação de Vírus/efeitos dos fármacos
alfa-Macroglobulinas/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Vírus da Encefalomiocardite/fisiologia
Herpesvirus Suídeo 1/fisiologia
Seres Humanos
Parvovirus Suíno/fisiologia
Reprodutibilidade dos Testes
Vírus Sindbis/fisiologia
Suínos
Fatores de Tempo
Células Vero
Vírus da Estomatite Vesicular Indiana/efeitos dos fármacos
Vírus da Estomatite Vesicular Indiana/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Proteins); 0 (Cohn fraction IV); 0 (alpha-Macroglobulins); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170418
[Lr] Data última revisão:
170418
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


  10 / 2487 MEDLINE  
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[PMID]:28214681
[Au] Autor:Liang C; Tong W; Zheng H; Liu F; Wu J; Li G; Zhou EM; Tong G
[Ad] Endereço:College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi, China; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
[Ti] Título:A high-temperature passaging attenuated Pseudorabies vaccine protects piglets completely against emerging PRV variant.
[So] Source:Res Vet Sci;112:109-115, 2017 Jun.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Emerging variant of pseudorabies virus (PRV) have evaded the antiviral immunity of commercially available PRV vaccine and have led to PRV outbreaks in Chinese pig farms. Here, we attenuated a PRV variant strain by serial passages in vitro and evaluate the protective efficacy of the attenuated strain as a vaccine candidate. The virulent PRV variant strain JS-2012 was continuously passaged in Vero cells at 40°C and attenuated rapidly. After 90 passages in Vero cells, the passaged virus lost its ability to cause death in 2-week-old piglets. The 120th passage virus was avirulent in the sucking piglets. An attenuated strain, JS-2012-F120 derived from the 120th passage virus by three rounds of plaque cloning grew better than its parent strain JS-2012 in Vero cells and showed notably different cytopathic effects and plaque morphology from JS-2012. PCR combined with sequence analysis showed that JS-2012-F120 contained a 2307-bp deletion covering nucleotide 487 of gE gene to 531 of US2 gene. After inoculation with JS-2012-F120, young piglets were completely protected from challenge with the classical and emerging virulent PRVs. Moreover, the piglets did not develop specific gE antibodies. Thus, JS-2012-F120 appears to be a promising marker vaccine to control PRV variant circulating in Chinese pig farms, and the high-temperature passaging in vitro was an efficient method to attenuated alphaherpesvirus.
[Mh] Termos MeSH primário: Herpesvirus Suídeo 1/genética
Vacinas contra Pseudorraiva/imunologia
Pseudorraiva/prevenção & controle
Doenças dos Suínos/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Surtos de Doenças
Suínos
Doenças dos Suínos/virologia
Temperatura Ambiente
Vacinação
Vacinas Atenuadas/imunologia
Proteínas do Envelope Viral/genética
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Pseudorabies Vaccines); 0 (Vaccines, Attenuated); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170220
[St] Status:MEDLINE



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