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[PMID]:28036408
[Au] Autor:Pozo F; Juste J; Vázquez-Morón S; Aznar-López C; Ibáñez C; Garin I; Aihartza J; Casas I; Tenorio A; Echevarría JE
[Ad] Endereço:Virology Section, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
[Ti] Título:Identification of Novel Betaherpesviruses in Iberian Bats Reveals Parallel Evolution.
[So] Source:PLoS One;11(12):e0169153, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A thorough search for bat herpesviruses was carried out in oropharyngeal samples taken from most of the bat species present in the Iberian Peninsula from the Vespertilionidae, Miniopteridae, Molossidae and Rhinolophidae families, in addition to a colony of captive fruit bats from the Pteropodidae family. By using two degenerate consensus PCR methods targeting two conserved genes, distinct and previously unrecognized bat-hosted herpesviruses were identified for the most of the tested species. All together a total of 42 potentially novel bat herpesviruses were partially characterized. Thirty-two of them were tentatively assigned to the Betaherpesvirinae subfamily while the remaining 10 were allocated into the Gammaherpesvirinae subfamily. Significant diversity was observed among the novel sequences when compared with type herpesvirus species of the ICTV-approved genera. The inferred phylogenetic relationships showed that most of the betaherpesviruses sequences fell into a well-supported unique monophyletic clade and support the recognition of a new betaherpesvirus genus. This clade is subdivided into three major clades, corresponding to the families of bats studied. This supports the hypothesis of a species-specific parallel evolution process between the potentially new betaherpesviruses and their bat hosts. Interestingly, two of the betaherpesviruses' sequences detected in rhinolophid bats clustered together apart from the rest, closely related to viruses that belong to the Roseolovirus genus. This suggests a putative third roseolo lineage. On the contrary, no phylogenetic structure was detected among several potentially novel bat-hosted gammaherpesviruses found in the study. Remarkably, all of the possible novel bat herpesviruses described in this study are linked to a unique bat species.
[Mh] Termos MeSH primário: Betaherpesvirinae/crescimento & desenvolvimento
Betaherpesvirinae/genética
Quirópteros/virologia
DNA Viral/genética
Gammaherpesvirinae/classificação
Gammaherpesvirinae/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Betaherpesvirinae/classificação
Betaherpesvirinae/isolamento & purificação
Evolução Biológica
Gammaherpesvirinae/isolamento & purificação
Variação Genética/genética
Filogenia
Reação em Cadeia da Polimerase
Portugal
Roseolovirus/classificação
Roseolovirus/genética
Alinhamento de Sequência
Análise de Sequência de DNA
Espanha
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161231
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169153


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[PMID]:25658336
[Au] Autor:Humphreys AF; Tan J; Peng R; Benton SM; Qin X; Worley KC; Mikulski RL; Chow DC; Palzkill TG; Ling PD
[Ad] Endereço:Center for Comparative Medicine, Baylor College of Medicine, Houston, Texas, United States of America.
[Ti] Título:Generation and characterization of antibodies against Asian elephant (Elephas maximus) IgG, IgM, and IgA.
[So] Source:PLoS One;10(2):e0116318, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Asian elephant (Elephas maximus) immunity is poorly characterized and understood. This gap in knowledge is particularly concerning as Asian elephants are an endangered species threatened by a newly discovered herpesvirus known as elephant endotheliotropic herpesvirus (EEHV), which is the leading cause of death for captive Asian elephants born after 1980 in North America. While reliable diagnostic assays have been developed to detect EEHV DNA, serological assays to evaluate elephant anti-EEHV antibody responses are lacking and will be needed for surveillance and epidemiological studies and also for evaluating potential treatments or vaccines against lethal EEHV infection. Previous studies have shown that Asian elephants produce IgG in serum, but they failed to detect IgM and IgA, further hampering development of informative serological assays for this species. To begin to address this issue, we determined the constant region genomic sequence of Asian elephant IgM and obtained some limited protein sequence information for putative serum IgA. The information was used to generate or identify specific commercial antisera reactive against IgM and IgA isotypes. In addition, we generated a monoclonal antibody against Asian elephant IgG. These three reagents were used to demonstrate that all three immunoglobulin isotypes are found in Asian elephant serum and milk and to detect antibody responses following tetanus toxoid booster vaccination or antibodies against a putative EEHV structural protein. The results indicate that these new reagents will be useful for developing sensitive and specific assays to detect and characterize elephant antibody responses for any pathogen or vaccine, including EEHV.
[Mh] Termos MeSH primário: Anticorpos Anti-Idiotípicos/imunologia
Betaherpesvirinae/imunologia
Elefantes/imunologia
Espécies em Perigo de Extinção
Infecções por Herpesviridae/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Cromatografia em Gel
Primers do DNA/genética
Eletroforese em Gel de Poliacrilamida
Ensaio de Imunoadsorção Enzimática
Infecções por Herpesviridae/imunologia
Imunoglobulina A/genética
Imunoglobulina A/imunologia
Imunoglobulina G/imunologia
Imunoglobulina M/genética
Imunoglobulina M/imunologia
Dados de Sequência Molecular
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Anti-Idiotypic); 0 (DNA Primers); 0 (Immunoglobulin A); 0 (Immunoglobulin G); 0 (Immunoglobulin M)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150207
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0116318


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[PMID]:25231309
[Au] Autor:Zong JC; Latimer EM; Long SY; Richman LK; Heaggans SY; Hayward GS
[Ad] Endereço:Viral Oncology Program, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
[Ti] Título:Comparative genome analysis of four elephant endotheliotropic herpesviruses, EEHV3, EEHV4, EEHV5, and EEHV6, from cases of hemorrhagic disease or viremia.
[So] Source:J Virol;88(23):13547-69, 2014 Dec.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: The genomes of three types of novel endotheliotropic herpesviruses (elephant endotheliotropic herpesvirus 1A [EEHV1A], EEHV1B, and EEHV2) associated with lethal hemorrhagic disease in Asian elephants have been previously well characterized and assigned to a new Proboscivirus genus. Here we have generated 112 kb of DNA sequence data from segments of four more types of EEHV by direct targeted PCR from blood samples or necropsy tissue samples from six viremic elephants. Comparative phylogenetic analysis of nearly 30 protein-encoding genes of EEHV5 and EEHV6 show that they diverge uniformly by nearly 20% from their closest relatives, EEHV2 and EEHV1A, respectively, and are likely to have similar overall gene content and genome organization. In contrast, seven EEHV3 and EEHV4 genes analyzed differ from those of all other EEHVs by 37% and have a G+C content of 63% compared to just 42% for the others. Three strains of EEHV5 analyzed clustered into two partially chimeric subgroups EEHV5A and EEHV5B that diverge by 19% within three small noncontiguous segments totaling 6.2 kb. We conclude that all six EEHV types should be designated as independent species within a proposed new fourth Deltaherpesvirinae subfamily of mammalian herpesviruses. These virus types likely initially diverged close to 100 million years ago when the ancestors of modern elephants split from all other placental mammals and then evolved into two major branches with high- or low-G+C content about 35 million years ago. Later additional branching events subsequently generated three paired sister taxon lineages of which EEHV1 plus EEHV6, EEHV5 plus EEHV2, and EEHV4 plus EEHV3 may represent Asian and African elephant versions, respectively. IMPORTANCE: One of the factors threatening the long-term survival of endangered Asian elephants in both wild range countries and in captive breeding populations in zoos is a highly lethal hemorrhagic herpesvirus disease that has killed at least 70 young Asian elephants worldwide. The genomes of the first three types of EEHVs (or probosciviruses) identified have been partially characterized in the preceding accompanying paper (L. K. Richman, J.-C. Zong, E. M. Latimer, J. Lock, R. C. Fleischer, S. Y. Heaggans, and G. S. Hayward, J. Virol. 88:13523-13546, 2014, http://dx.doi.org/10.1128/JVI.01673-14). Here we have used PCR DNA sequence analysis from multiple segments of DNA amplified directly from blood or necropsy tissue samples of six more selected cases of hemorrhagic disease to partially characterize four other types of EEHVs from either Asian or African elephants. We propose that all six types and two chimeric subtypes of EEHV belong to multiple lineages of both AT-rich and GC-rich branches within a new subfamily to be named the Deltaherpesvirinae, which evolved separately from all other mammalian herpesviruses about100 million years ago.
[Mh] Termos MeSH primário: Betaherpesvirinae/classificação
Betaherpesvirinae/isolamento & purificação
Sangue/virologia
Variação Genética
Infecções por Herpesviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Composição de Bases
Betaherpesvirinae/genética
Análise por Conglomerados
DNA Viral/química
DNA Viral/genética
Elefantes
Infecções por Herpesviridae/virologia
Dados de Sequência Molecular
Fases de Leitura Aberta
Filogenia
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140919
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.01675-14


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[PMID]:25231304
[Au] Autor:Pellett PE
[Ad] Endereço:Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan, USA ppellett@med.wayne.edu.
[Ti] Título:Trunkloads of viruses.
[So] Source:J Virol;88(23):13520-2, 2014 Dec.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Elephant populations are under intense pressure internationally from habitat destruction and poaching for ivory and meat. They also face pressure from infectious agents, including elephant endotheliotropic herpesvirus 1 (EEHV1), which kills ~20% of Asian elephants (Elephas maximus) born in zoos and causes disease in the wild. EEHV1 is one of at least six distinct EEHV in a phylogenetic lineage that appears to represent an ancient but newly recognized subfamily (the Deltaherpesvirinae) in the family Herpesviridae.
[Mh] Termos MeSH primário: Betaherpesvirinae/classificação
Betaherpesvirinae/isolamento & purificação
Sangue/virologia
Variação Genética
Infecções por Herpesviridae/veterinária
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:COMMENT; JOURNAL ARTICLE
[Em] Mês de entrada:1502
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140919
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.02359-14


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[PMID]:25231303
[Au] Autor:Richman LK; Zong JC; Latimer EM; Lock J; Fleischer RC; Heaggans SY; Hayward GS
[Ad] Endereço:Viral Oncology Program, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins School of Medicine, Baltimore, Maryland, USA National Elephant Herpesvirus Laboratory, Pathology Department, Smithsonian's National Zoo, Washington, DC, USA.
[Ti] Título:Elephant endotheliotropic herpesviruses EEHV1A, EEHV1B, and EEHV2 from cases of hemorrhagic disease are highly diverged from other mammalian herpesviruses and may form a new subfamily.
[So] Source:J Virol;88(23):13523-46, 2014 Dec.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: A family of novel endotheliotropic herpesviruses (EEHVs) assigned to the genus Proboscivirus have been identified as the cause of fatal hemorrhagic disease in 70 young Asian elephants worldwide. Although EEHV cannot be grown in cell culture, we have determined a total of 378 kb of viral genomic DNA sequence directly from clinical tissue samples from six lethal cases and two survivors. Overall, the data obtained encompass 57 genes, including orthologues of 32 core genes common to all herpesviruses, 14 genes found in some other herpesviruses, plus 10 novel genes, including a single large putative transcriptional regulatory protein (ORF-L). On the basis of differences in gene content and organization plus phylogenetic analyses of conserved core proteins that have just 20% to 50% or less identity to orthologues in other herpesviruses, we propose that EEHV1A, EEHV1B, and EEHV2 could be considered a new Deltaherpesvirinae subfamily of mammalian herpesviruses that evolved as an intermediate branch between the Betaherpesvirinae and Gammaherpesvirinae. Unlike cytomegaloviruses, EEHV genomes encode ribonucleotide kinase B subunit (RRB), thymidine kinase (TK), and UL9-like origin binding protein (OBP) proteins and have an alphaherpesvirus-like dyad symmetry Ori-Lyt domain. They also differ from all known betaherpesviruses by having a 40-kb large-scale inversion of core gene blocks I, II, and III. EEHV1 and EEHV2 DNA differ uniformly by more than 25%, but EEHV1 clusters into two major subgroups designated EEHV1A and EEHV1B with ancient partially chimeric features. Whereas large segments are nearly identical, three nonadjacent loci totaling 15 kb diverge by between 21 and 37%. One strain of EEHV1B analyzed is interpreted to be a modern partial recombinant with EEHV1A. IMPORTANCE: Asian elephants are an endangered species whose survival is under extreme pressure in wild range countries and whose captive breeding populations in zoos are not self-sustaining. In 1999, a novel class of herpesviruses called EEHVs was discovered. These viruses have caused a rapidly lethal hemorrhagic disease in 20% of all captive Asian elephant calves born in zoos in the United States and Europe since 1980. The disease is increasingly being recognized in Asian range countries as well. These viruses cannot be grown in cell culture, but by direct PCR DNA sequence analysis from segments totaling 15 to 30% of the genomes from blood or necropsy tissue from eight different cases, we have determined that they fall into multiple types and chimeric subtypes of a novel Proboscivirus genus, and we propose that they should also be classified as the first examples of a new mammalian herpesvirus subfamily named the Deltaherpesvirinae.
[Mh] Termos MeSH primário: Betaherpesvirinae/classificação
Betaherpesvirinae/isolamento & purificação
Variação Genética
Infecções por Herpesviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Betaherpesvirinae/genética
Análise por Conglomerados
DNA Viral/química
DNA Viral/genética
Elefantes
Infecções por Herpesviridae/virologia
Dados de Sequência Molecular
Fases de Leitura Aberta
Filogenia
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140919
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.01673-14


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[PMID]:25195484
[Au] Autor:Nair RG; Salajegheh A; Itthagarun A; Pakneshan S; Brennan MT; Samaranayake LP
[Ti] Título:Orofacial viral infections--an update for clinicians.
[So] Source:Dent Update;41(6):518-20, 522-4, 2014 Jul-Aug.
[Is] ISSN:0305-5000
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Orofacial viral infections may be less common but appear in different clinical forms. Often these infections get initially treated by antibiotics which obviously will have limited or no effect. The authors review the current concepts of orofacial viral infections, causative agents, their classification and clinical manifestations and a basis for treatment. CLINICAL RELEVANCE: Most viral infections do not require any specific treatment except in patients who are immunosuppressed or immunodeficient. Appropriate diagnosis and timely management of orofacial viral lesions are important irrespective of whether it is localized or a manifestation of a systemic infection.
[Mh] Termos MeSH primário: Infecções por Herpesviridae/diagnóstico
Doenças da Boca/virologia
Infecções por Papillomavirus/diagnóstico
[Mh] Termos MeSH secundário: Alphaherpesvirinae/classificação
Alphapapillomavirus/classificação
Betaherpesvirinae/classificação
Infecções por Coxsackievirus/diagnóstico
Gammaherpesvirinae/classificação
Infecções por HIV/diagnóstico
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1409
[Cu] Atualização por classe:140908
[Lr] Data última revisão:
140908
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:140909
[St] Status:MEDLINE


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[PMID]:24725936
[Au] Autor:Furuse Y; Dastjerdi A; Seilern-Moy K; Steinbach F; Cullen BR
[Ad] Endereço:Department of Molecular Genetics & Microbiology and Center for Virology, Duke University Medical Center, Durham, NC 27710, USA.
[Ti] Título:Analysis of viral microRNA expression by elephant endotheliotropic herpesvirus 1.
[So] Source:Virology;454-455:102-8, 2014 Apr.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Elephant endotheliotropic herpesvirus 1 (EEHV1), a member of the Betaherpesvirinae subfamily, has recently emerged as an important viral pathogen of Asian elephants that can cause a severe, often fatal, hemorrhagic disease. EEHV1 does not replicate in culture and little is currently known about the molecular biology of this emerging pathogen, with the notable exception of its genomic DNA sequence. Here, we have used small RNA deep sequencing to determine whether EEHV1, like other human and murine betaherpesviruses, expresses viral microRNAs in infected tissues in vivo. Our data provide evidence supporting the existence of at least three novel viral microRNAs encoded by EEHV1 and one of these, miR-E3-5p, is shown to repress target mRNA expression. Moreover, miR-E3-5p expression was readily detectable in tissue samples derived from two infected elephants, including in whole blood. These data shed new light on the biology of EEHV1 and identify small RNAs that have the potential to be useful in the diagnosis of sub-clinical infections in captive Asian and African elephants.
[Mh] Termos MeSH primário: Betaherpesvirinae/genética
Expressão Gênica
Infecções por Herpesviridae/veterinária
MicroRNAs/biossíntese
RNA Viral/biossíntese
[Mh] Termos MeSH secundário: Animais
Betaherpesvirinae/isolamento & purificação
Elefantes
Perfilação da Expressão Gênica
Infecções por Herpesviridae/virologia
Sequenciamento de Nucleotídeos em Larga Escala
MicroRNAs/genética
RNA Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MicroRNAs); 0 (RNA, Viral)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:140414
[Lr] Data última revisão:
140414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140415
[St] Status:MEDLINE


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[PMID]:24132949
[Au] Autor:Yamamoto Y; Morooka M; Hashimoto S; Ihra M; Yoshikawa T
[Ad] Endereço:Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
[Ti] Título:Analysis of the shedding of three ß-herpesviruses in urine and saliva of children with renal disease.
[So] Source:J Med Virol;86(3):505-11, 2014 Mar.
[Is] ISSN:1096-9071
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and 7 (HHV-7) are important pathogens in immunocompromised patients. To elucidate the kinetics of the three ß-herpesviruses in saliva and urine samples were collected serially from children with renal diseases. Twenty children with renal diseases were enrolled in this study. A total of 240 saliva and urine samples were collected monthly from the patients over a 1-year period. Viral DNAs loads were measured by real-time PCR. In 10 CMV seropositive patients CMV DNA was detected rarely in saliva and CMV DNA load was lower than the other two ß-herpesviruses DNA loads. All patients were seropositive for HHV-6B and the virus was detected frequently in saliva. Two of 20 patients were HHV-7 seronegative. High copies of viral DNA were detected continuously in saliva of the HHV-7 seropositive patients. Although neither CMV nor HHV-6B DNA load was different among the three renal diseases, HHV-7 DNA load was different among the diseases (P = 0.039). HHV-6B DNA loads were significantly higher in patients with immunosuppressive treatment compared to those without treatment (P = 0.013). Although CMV DNA was detected in urine samples collected from 5 of 10 CMV seropositive patients, HHV-6B and HHV-7 DNA were detected at relatively low frequencies in urine. No remarkable temporal associations between viral DNA excretion and proteinuria or immunosuppressive treatment were demonstrated. The pattern of viral DNA excretion in saliva and urine were different among the three viruses. No temporal correlation was observed between viral infection and renal diseases.
[Mh] Termos MeSH primário: Betaherpesvirinae/isolamento & purificação
Infecções por Herpesviridae/virologia
Nefropatias/virologia
Saliva/virologia
Urina/virologia
Eliminação de Partículas Virais
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
DNA Viral/genética
DNA Viral/isolamento & purificação
Feminino
Seres Humanos
Estudos Longitudinais
Masculino
Reação em Cadeia da Polimerase em Tempo Real
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1408
[Cu] Atualização por classe:140109
[Lr] Data última revisão:
140109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131018
[St] Status:MEDLINE
[do] DOI:10.1002/jmv.23782


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[PMID]:23552421
[Au] Autor:Wilkie GS; Davison AJ; Watson M; Kerr K; Sanderson S; Bouts T; Steinbach F; Dastjerdi A
[Ad] Endereço:MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.
[Ti] Título:Complete genome sequences of elephant endotheliotropic herpesviruses 1A and 1B determined directly from fatal cases.
[So] Source:J Virol;87(12):6700-12, 2013 Jun.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A highly lethal hemorrhagic disease associated with infection by elephant endotheliotropic herpesvirus (EEHV) poses a severe threat to Asian elephant husbandry. We have used high-throughput methods to sequence the genomes of the two genotypes that are involved in most fatalities, namely, EEHV1A and EEHV1B (species Elephantid herpesvirus 1, genus Proboscivirus, subfamily Betaherpesvirinae, family Herpesviridae). The sequences were determined from postmortem tissue samples, despite the data containing tiny proportions of viral reads among reads from a host for which the genome sequence was not available. The EEHV1A genome is 180,421 bp in size and consists of a unique sequence (174,601 bp) flanked by a terminal direct repeat (2,910 bp). The genome contains 116 predicted protein-coding genes, of which six are fragmented, and seven paralogous gene families are present. The EEHV1B genome is very similar to that of EEHV1A in structure, size, and gene layout. Half of the EEHV1A genes lack orthologs in other members of subfamily Betaherpesvirinae, such as human cytomegalovirus (genus Cytomegalovirus) and human herpesvirus 6A (genus Roseolovirus). Notable among these are 23 genes encoding type 3 membrane proteins containing seven transmembrane domains (the 7TM family) and seven genes encoding related type 2 membrane proteins (the EE50 family). The EE50 family appears to be under intense evolutionary selection, as it is highly diverged between the two genotypes, exhibits evidence of sequence duplications or deletions, and contains several fragmented genes. The availability of the genome sequences will facilitate future research on the epidemiology, pathogenesis, diagnosis, and treatment of EEHV-associated disease.
[Mh] Termos MeSH primário: Betaherpesvirinae/genética
Elefantes/virologia
Genoma Viral/genética
Infecções por Herpesviridae/veterinária
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Animais
Autopsia
Sequência de Bases
Betaherpesvirinae/classificação
Betaherpesvirinae/isolamento & purificação
DNA Viral/análise
DNA Viral/genética
DNA Viral/isolamento & purificação
Evolução Fatal
Feminino
Infecções por Herpesviridae/virologia
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Masculino
Dados de Sequência Molecular
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130405
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.00655-13


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[PMID]:23279912
[Au] Autor:Malkowska M; Kokoszynska K; Dymecka M; Rychlewski L; Wyrwicz LS
[Ad] Endereço:Laboratory of Bioinformatics and Biostatistics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, WK Roentgena 5, Warsaw, Poland. lucjan@bioinfo.pl
[Ti] Título:Alphaherpesvirinae and Gammaherpesvirinae glycoprotein L and CMV UL130 originate from chemokines.
[So] Source:Virol J;10:1, 2013 Jan 02.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Herpesviridae is a large family of DNA viruses divided into three subfamilies: Alpha-, Beta- and Gammaherpesvirinae. The process of herpesvirus transmission is mediated by a range of proteins, one of which is glycoprotein L (gL). Based on our analysis of the solved structures of HSV2 and EBV gH/gL complexes, we propose that Alphaherpesvirinae and Gammaherpesvirinae glycoprotein L and Betaherpesvirinae UL130 originate from chemokines. Herpes simplex virus type 2 gL and human cytomegalovirus homolog (UL130) adopt a novel C chemokine-like fold, while Epstein-Barr virus gL mimics a CC chemokine structure. Hence, it is possible that gL interface with specific chemokine receptors during the transmission of Herpesviridae. We conclude that the further understanding of the function of viral chemokine-like proteins in Herpesviridae infection may lead to development of novel prophylactic and therapeutic treatment.
[Mh] Termos MeSH primário: Alphaherpesvirinae/química
Betaherpesvirinae/química
Quimiocinas/química
Gammaherpesvirinae/química
Proteínas do Envelope Viral/química
[Mh] Termos MeSH secundário: Alphaherpesvirinae/genética
Sequência de Aminoácidos
Betaherpesvirinae/genética
Quimiocinas/genética
Evolução Molecular
Gammaherpesvirinae/genética
Modelos Moleculares
Dados de Sequência Molecular
Conformação Proteica
Alinhamento de Sequência
Proteínas do Envelope Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemokines); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:1401
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130103
[St] Status:MEDLINE
[do] DOI:10.1186/1743-422X-10-1



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