Base de dados : MEDLINE
Pesquisa : B04.280.410 [Categoria DeCS]
Referências encontradas : 619 [refinar]
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  1 / 619 MEDLINE  
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[PMID]:28874231
[Au] Autor:Li F; Xu L; Yang F
[Ad] Endereço:1​State Key Laboratory Breeding Base of Marine Genetic Resources; Fujian Key Laboratory of Marine Genetic Resources; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of State Oceanic Administration, T
[Ti] Título:Genomic characterization of a novel iridovirus from redclaw crayfish Cherax quadricarinatus: evidence for a new genus within the family Iridoviridae.
[So] Source:J Gen Virol;98(10):2589-2595, 2017 Oct.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel iridovirus, Cherax quadricarinatus iridovirus (CQIV), was identified from diseased C. quadricarinatus in 2014. This virus is considered as a new threat to crustacean aquaculture because it is lethal to both peneaid shrimp and crayfish. Here, we determined the complete genome sequence of CQIV. The double-stranded DNA genome is 165 695 bp in length with a G+C content of 34.6 %. A total of 178 open reading frames (ORFs) have been predicted, encoding hypothetical proteins ranging from 50 to 1327 amino acids. Forty-seven of these exhibit similarities to proteins of known functions. Phylogenetic analysis based on multiple alignments of conserved proteins shows that CQIV clusters with the members of the family Iridoviridae, but is placed in a distinct clade from all the five known genera. It indicates that CQIV may represent a new genus in the family Iridoviridae, for which we propose the name Cheraxvirus based on the host organism.
[Mh] Termos MeSH primário: Astacoidea/virologia
DNA Viral/genética
Genoma Viral/genética
Iridoviridae
[Mh] Termos MeSH secundário: Animais
Composição de Bases
Sequência de Bases
Iridoviridae/classificação
Iridoviridae/genética
Iridoviridae/isolamento & purificação
Fases de Leitura Aberta/genética
Análise de Sequência de DNA
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000904


  2 / 619 MEDLINE  
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[PMID]:28631595
[Au] Autor:Zhong Y; Fei C; Tang X; Zhan W; Sheng X
[Ad] Endereço:1​Laboratory of Pathology and Immunology of Aquatic Animals, KLM, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
[Ti] Título:A 32 kDa viral attachment protein of lymphocystis disease virus (LCDV) specifically interacts with a 27.8 kDa cellular receptor from flounder (Paralichthys olivaceus).
[So] Source:J Gen Virol;98(6):1477-1488, 2017 Jun.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The 27.8 kDa protein in flounder gill (FG) cells was previously proved to be a receptor specific for lymphocystis disease virus (LCDV) entry and infection. In this paper, a 32 kDa viral attachment protein (VAP) of LCDV specifically binding to the 27.8 kDa receptor (27.8R) was found by far-Western blotting coupled with monoclonal antibodies (MAbs) against 27.8R. The 32 kDa protein was confirmed to be encoded by the open reading frame (ORF) 038 gene in LCDV-C, and predicted to contain a putative transmembrane region, multiple N-myristoylation and glycosylation sites and phosphorylation motifs. The expression plasmid of pET-32a-ORF038 was constructed and the recombinant VAP (rVAP) was obtained. Rabbit polyclonal antibodies against the rVAP were prepared and could recognize the rVAP and 32 kDa protein in LCDV. Immunogold electron microscopy showed that the 32 kDa protein was located on the surface of LCDV particles. Immunofluorescence assay demonstrated that the rVAP could bind to the 27.8R on the cell membrane of the FG monolayer and the anti-27.8R MAbs could block the rVAP binding. Pre-incubation of the rVAP with FG cells before LCDV infection, or pre-incubation of LCDV with the antibodies against the rVAP, could significantly decrease the LCDV copy numbers (P<0.05) and delay the emergence of cytopathic effects in FG cells in a dose-dependent manner. These results indicated for the first time that the 32 kDa protein functioned as an attachment protein for the initial attachment and entry of LCDV, and the interaction of the 32 kDa VAP with the 27.8R-initiated LCDV infection.
[Mh] Termos MeSH primário: Linguado/virologia
Interações Hospedeiro-Patógeno
Iridoviridae/fisiologia
Receptores de Superfície Celular/metabolismo
Proteínas do Envelope Viral/metabolismo
Ligação Viral
[Mh] Termos MeSH secundário: Animais
Far-Western Blotting
Microscopia Imunoeletrônica
Ligação Proteica
Receptores de Superfície Celular/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Cell Surface); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000805


  3 / 619 MEDLINE  
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[PMID]:28555546
[Au] Autor:Chinchar VG; Hick P; Ince IA; Jancovich JK; Marschang R; Qin Q; Subramaniam K; Waltzek TB; Whittington R; Williams T; Zhang QY; Ictv Report Consortium
[Ad] Endereço:1​Department of Microbiology, University of Mississippi Medical Center, Jackson, MS 39216, USA.
[Ti] Título:ICTV Virus Taxonomy Profile: Iridoviridae.
[So] Source:J Gen Virol;98(5):890-891, 2017 May.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Iridoviridae is a family of large, icosahedral viruses with double-stranded DNA genomes ranging in size from 103 to 220 kbp. Members of the subfamily Alphairidovirinae infect ectothermic vertebrates (bony fish, amphibians and reptiles), whereas members of the subfamily Betairidovirinae mainly infect insects and crustaceans. Infections can be either covert or patent, and in vertebrates they can lead to high levels of mortality among commercially and ecologically important fish and amphibians. This is a summary of the current International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Iridoviridae, which is available at www.ictv.global/report/iridoviridae.
[Mh] Termos MeSH primário: Iridoviridae/classificação
Iridoviridae/isolamento & purificação
[Mh] Termos MeSH secundário: Anfíbios/virologia
Animais
Crustáceos/virologia
DNA Viral/genética
Peixes/virologia
Especificidade de Hospedeiro
Insetos/virologia
Iridoviridae/ultraestrutura
Répteis/virologia
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000818


  4 / 619 MEDLINE  
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[PMID]:28399906
[Au] Autor:Valverde EJ; Borrego JJ; Sarasquete MC; Ortiz-Delgado JB; Castro D
[Ad] Endereço:Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Campus Universitario Teatinos, Malaga, Spain.
[Ti] Título:Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata).
[So] Source:Vet Res;48(1):21, 2017 Apr 11.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The lymphocystis disease (LCD), the main viral pathology described in cultured gilthead seabream (Sparus aurata), is a self-limiting condition characterized by the appearance of hypertrophied fibroblasts (named lymphocysts) in the connective tissue of fish, primarily in the skin and fins. The causative agent of the disease is the Lymphocystis disease virus (LCDV), a member of the Iridoviridae family. In the present study, LCDV genome and transcripts were detected by real-time PCR in caudal fin, as well as in several internal organs, such as intestine, liver, spleen, kidney and brain, from asymptomatic, diseased and recovered gilthead seabream juveniles. These results indicate that the LCDV has a broad range tissue tropism, and can establish a systemic infection, even in subclinically infected fish. As showed by in situ hybridization, the permissive cells for LCDV infection seem to be fibroblasts, hepatocytes and cells of the mononuclear phagocyte system. Histopathological alterations associated with LCD were observed in all the organs analysed, including necrotic changes in liver and kidney, inflammatory response in the intestine submucosa or brain haemorrhage, although lymphocysts were only detected in the dermis of the caudal fin. Nevertheless, these histological changes were reverted in recovered animals.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/veterinária
Doenças dos Peixes/virologia
Iridoviridae/fisiologia
Dourada/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Vírus de DNA/patologia
Infecções por Vírus de DNA/virologia
DNA Viral/genética
Doenças dos Peixes/patologia
Hibridização In Situ/veterinária
Iridoviridae/genética
Carga Viral/veterinária
Replicação Viral/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE
[do] DOI:10.1186/s13567-017-0428-3


  5 / 619 MEDLINE  
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[PMID]:28263880
[Au] Autor:Zhang M; Yue B; Zhang AH; Wang GH; Liu Y; Zhou S; Cheng SF; Li NQ
[Ad] Endereço:Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China.
[Ti] Título:TC38, a teleost TFPI-2 peptide that kills bacteria via penetration of the cell membrane and interaction with nucleic acids.
[So] Source:Fish Shellfish Immunol;64:104-110, 2017 May.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tissue factor pathway inhibitor 2 (TFPI-2) is an analog of TFPI-1 and a potent endogenous inhibitor of tissue factor (TF)-mediated blood coagulation. Recent reports have proven that the C-terminal of TFPI-2 peptides in humans and several other vertebrates possesses antibacterial activity against Gram-positive and Gram-negative bacteria. In our previous study, we reported that the TFPI-2 peptide, TC38 in tongue sole (Cynoglossus semilaevis) was active against Micrococcus luteus. In this study, we further examine the antimicrobial spectrum, mechanism of action, and function of TC38 in tongue sole. Our results indicate that TC38 is active against the Gram-negative bacteria Vibrio ichthyoenteri, Vibrio litoralis, Vibrio parahaemolyticus, and Vibrio vulnificus, as well as the fish Megalocytivirus, infectious spleen and kidney necrosis virus (ISKNV). The mechanism of action of TC38 against V. vulnificus was explored. The results showed that TC38 killed V. vulnificus cells without lysis of the cell membrane. FITC-labeled TC38 was able to penetrate the cell membrane and bind to DNA and RNA, then disrupt cellular function, eventually leading to cell death. Administration of TC38 to tongue sole significantly improved its defense against V. vulnificus infection. Overall, these results indicate that TC38 is a novel peptide with a broad antimicrobial spectrum. Furthermore, the unique action of TC38 against V. vulnificus adds new insights to the mechanism of action of vertebrate TFPI peptides. Moreover, TC38 is an interesting antimicrobial agent that could be useful in the fight against pathogenic invasion in aquaculture.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/veterinária
Doenças dos Peixes/genética
Proteínas de Peixes/genética
Linguados
Glicoproteínas/genética
Vibrioses/veterinária
[Mh] Termos MeSH secundário: Animais
Membrana Celular/microbiologia
Membrana Celular/virologia
Infecções por Vírus de DNA/genética
Infecções por Vírus de DNA/imunologia
Infecções por Vírus de DNA/virologia
Doenças dos Peixes/imunologia
Doenças dos Peixes/microbiologia
Doenças dos Peixes/virologia
Proteínas de Peixes/metabolismo
Glicoproteínas/metabolismo
Iridoviridae/fisiologia
Ácidos Nucleicos/metabolismo
Distribuição Aleatória
Vibrio/fisiologia
Vibrioses/genética
Vibrioses/imunologia
Vibrioses/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Glycoproteins); 0 (Nucleic Acids); 0 (tissue-factor-pathway inhibitor 2)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170307
[St] Status:MEDLINE


  6 / 619 MEDLINE  
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[PMID]:27856327
[Au] Autor:Li C; Fu X; Lin Q; Liu L; Liang H; Huang Z; Li N
[Ad] Endereço:Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou, 510380, China; College of Fisheries and Life Science, Shanghai Ocean
[Ti] Título:Autophagy promoted infectious kidney and spleen necrosis virus replication and decreased infectious virus yields in CPB cell line.
[So] Source:Fish Shellfish Immunol;60:25-32, 2017 Jan.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Autophagy plays important functions in viral replication and pathogenesis. In this study, we investigated the role of autophagy in the replication of infectious kidney and spleen necrosis virus (ISKNV), an agent that has caused devastating losses in Chinese perch (Siniperca chuatsi) industry. We found that ISKNV infection triggered the complete autophagic process, as demonstrated by microtubule-associated protein 1 light chain 3B II (LC3B-II) conversion, an increased accumulation of punctate GFP-LC3-expressing cells, a higher number of autophagosome-double-membrane vesicles in the cytoplasm, and increased levels of autophagic flux in CPB cells. Then, we investigated the role of autophagy in the process of ISKNV replication. Results showed that inducing autophagy by rapamycin promoted ISKNV replication and proteins synthesis but decreased extracellular virus yields. While, blocking autophagosome-lysosome fusion by chloroquine (CQ) promoted infectious virus yields in culture supernatant. These results offer insight into the complex interactions between ISKNV and host cell, providing new insights into viral pathogenesis and antiviral treatment strategies.
[Mh] Termos MeSH primário: Autofagia
Infecções por Vírus de DNA/veterinária
Doenças dos Peixes/virologia
Iridoviridae/fisiologia
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Linhagem Celular/virologia
Infecções por Vírus de DNA/virologia
Percas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


  7 / 619 MEDLINE  
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[PMID]:27840169
[Au] Autor:He SW; Zhang J; Li NQ; Zhou S; Yue B; Zhang M
[Ad] Endereço:Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China.
[Ti] Título:A TFPI-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, Cynoglossus semilaevis.
[So] Source:Fish Shellfish Immunol;60:466-473, 2017 Jan.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tissue factor pathway inhibitor 1 (TFPI-1) is a serine protease inhibitor that inhibits tissue factor (TF)-mediated coagulation. The C-terminal region of TFPI-1 could be cleaved off and proved to be antimicrobial against a broad-spectrum of microorganism. In a previous study, a C-terminal peptide, TC24 (with 24 amino acids), derived from tongue sole (Cynoglossus semilaevis) TFPI-1, was synthesized and found antibacterial against Micrococcus luteus. In the present study, the antibacterial spectrum and the action mode of TC24 was further examined, and its in vivo function was analyzed. Our results showed that TC24 also possesses bactericidal activity against Staphylococcus aureus and Vibrio vulnificus. During its interaction with the target bacterial cells, TC24 destroyed cell membrane integrity, penetrated into the cytoplasm, and induced degradation of genomic DNA and total RNA. In vivo study showed that administration of tongue sole with TC24 before bacterial and viral infection significantly reduced pathogen dissemination and replication in tissues. These results indicated that TC24 is a novel antimicrobial peptide against bacterial and viral pathogens, and that the observed effect of TC24 on bacterial RNA adds new insights to the action mechanism of fish antimicrobial peptides. Moreover, TC24 may play an important role in fighting pathogenic infection in aquaculture.
[Mh] Termos MeSH primário: Doenças dos Peixes/genética
Proteínas de Peixes/genética
Linguados
Lipoproteínas/genética
[Mh] Termos MeSH secundário: Animais
Infecções por Vírus de DNA/genética
Infecções por Vírus de DNA/imunologia
Infecções por Vírus de DNA/veterinária
Infecções por Vírus de DNA/virologia
Doenças dos Peixes/imunologia
Doenças dos Peixes/microbiologia
Doenças dos Peixes/virologia
Proteínas de Peixes/metabolismo
Bactérias Gram-Negativas/fisiologia
Infecções por Bactérias Gram-Negativas/genética
Infecções por Bactérias Gram-Negativas/imunologia
Infecções por Bactérias Gram-Negativas/microbiologia
Infecções por Bactérias Gram-Negativas/veterinária
Bactérias Gram-Positivas/fisiologia
Infecções por Bactérias Gram-Positivas/genética
Infecções por Bactérias Gram-Positivas/imunologia
Infecções por Bactérias Gram-Positivas/microbiologia
Infecções por Bactérias Gram-Positivas/veterinária
Iridoviridae/fisiologia
Lipoproteínas/metabolismo
Análise de Sequência de DNA/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Lipoproteins); 0 (lipoprotein-associated coagulation inhibitor)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161115
[St] Status:MEDLINE


  8 / 619 MEDLINE  
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[PMID]:27709436
[Au] Autor:Valverde EJ; Cano I; Castro D; Paley RK; Borrego JJ
[Ad] Endereço:Departamento de Microbiología, Universidad de Málaga, 29071, Málaga, Spain.
[Ti] Título:Rapid and Sensitive Detection of Lymphocystis Disease Virus Genotype VII by Loop-Mediated Isothermal Amplification.
[So] Source:Food Environ Virol;9(1):114-122, 2017 Mar.
[Is] ISSN:1867-0342
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lymphocystis disease virus (LCDV) infections have been described in gilthead seabream (Sparus aurata L.) and Senegalese sole (Solea senegalensis, Kaup), two of the most important marine fish species in the Mediterranean aquaculture. In this study, a rapid, specific, and sensitive detection method for LCDV genotype VII based on loop-mediated isothermal amplification (LAMP) was developed. The LAMP assay, performed using an apparatus with real-time amplification monitoring, was able to specifically detect LCDV genotype VII from clinically positive samples in less than 12 min. In addition, the assay allowed the detection of LCDV in all asymptomatic carrier fish analysed, identified by qPCR, showing an analytical sensitivity of ten copies of viral DNA per reaction. The LCDV LAMP assay has proven to be a promising diagnostic method that can be used easily in fish farms to detect the presence and spread of this iridovirus.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/veterinária
Doenças dos Peixes/virologia
Iridoviridae/isolamento & purificação
Técnicas de Amplificação de Ácido Nucleico/métodos
[Mh] Termos MeSH secundário: Animais
Primers do DNA/genética
Infecções por Vírus de DNA/diagnóstico
Infecções por Vírus de DNA/virologia
Doenças dos Peixes/diagnóstico
Genótipo
Iridoviridae/classificação
Iridoviridae/genética
Dourada/virologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161007
[St] Status:MEDLINE
[do] DOI:10.1007/s12560-016-9265-1


  9 / 619 MEDLINE  
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[PMID]:27523084
[Au] Autor:Jin JW; Kim YC; Hong S; Kim MS; Jeong JB; Jeong HD
[Ad] Endereço:Namhae Fisheries Hatchery Station, Korea Fisheries Resources Agency, Wando, South Korea.
[Ti] Título:Cloning and expression analysis of innate immune genes from red sea bream to assess different susceptibility to megalocytivirus infection.
[So] Source:J Fish Dis;40(4):583-595, 2017 Apr.
[Is] ISSN:1365-2761
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:As suggested by the Office International des Epizooties (OIE), fishes belonging to the genus Oplegnathus are more sensitive to megalocytivirus infection than other fish species including red sea bream (Pagrus major). To assess the roles of the innate immune response to these different susceptibilities, we cloned the genes encoding inflammatory factors including IL-8 and COX-2, and the antiviral factor like Mx from red sea bream for the first time and performed phylogenetic and structural analysis. Analysed expression levels of IL-1ß, IL-8 and COX-2 and the antiviral factor like Mx genes performed with in vivo challenge experiment showed no difference in inflammatory gene expression or respiratory burst activity between red sea bream and rock bream (Oplegnathus fasciatus). However, the Mx gene expression levels in red sea bream were markedly higher than those in rock bream, suggesting the importance of type I interferon (IFN)-induced proteins, particularly Mx, during megalocytivirus infection, rather than inflammation-related genes. The in vitro challenge experiments using embryonic primary cultures derived from both fish species showed no difference in cytopathic effects (CPE), viral replication profiles, and inflammatory and Mx gene expression pattern between the two fish species.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/veterinária
Doenças dos Peixes/genética
Regulação da Expressão Gênica
Predisposição Genética para Doença/genética
Imunidade Inata/genética
Iridoviridae/imunologia
Dourada
[Mh] Termos MeSH secundário: Animais
Clonagem Molecular
Infecções por Vírus de DNA/genética
Infecções por Vírus de DNA/imunologia
Infecções por Vírus de DNA/virologia
Doenças dos Peixes/imunologia
Doenças dos Peixes/virologia
Proteínas de Peixes/genética
Proteínas de Peixes/metabolismo
Perfilação da Expressão Gênica
Análise de Sequência de DNA/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160816
[St] Status:MEDLINE
[do] DOI:10.1111/jfd.12537


  10 / 619 MEDLINE  
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[PMID]:27334576
[Au] Autor:Rimmer AE; Whittington RJ; Tweedie A; Becker JA
[Ad] Endereço:Faculty of Veterinary Science, School of Life and Environmental Sciences, The University of Sydney, Camden, NSW, Australia.
[Ti] Título:Susceptibility of a number of Australian freshwater fishes to dwarf gourami iridovirus (Infectious spleen and kidney necrosis virus).
[So] Source:J Fish Dis;40(3):293-310, 2017 Mar.
[Is] ISSN:1365-2761
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Megalocytiviruses cause high mortality diseases that have seriously impacted aquaculture, with the most frequent outbreaks occurring in East and South-East Asia. The international trade of juvenile fish for food and ornamental aquaculture has aided the spread of these viruses, which have spread to Europe and Australia and other regions. Australian freshwater fishes were examined for susceptibility to infection with the exotic megalocytivirus, dwarf gourami iridovirus (DGIV), which belongs to a group with the type species, Infectious spleen and kidney necrosis virus (ISKNV). Fish were held at 23 ± 1 °C and challenged by intraperitoneal (IP) injection or by cohabitation with Murray cod, Maccullochella peelii (Mitchell) infected with DGIV. A species was deemed to be susceptible to DGIV based on evidence of viral replication, as determined by qPCR, and megalocytic inclusion bodies observed histologically. Horizontal transmission occurred between infected Murray cod and golden perch, Macquaria ambigua (Richardson), Macquarie perch, Macquaria australasica (Cuvier) and Murray cod. This indicated that DGIV shed from infected fish held at 23 °C can survive in fresh water and subsequently infect these naïve fish. Further, DGIV administered IP was highly pathogenic to golden perch, Macquarie perch and Murray cod. Compared to these species, the susceptibility of southern pygmy perch, Nannoperca australis (Gunther) was lower. Freshwater catfish (dewfish), Tandanus tandanus (Mitchell), were not susceptible under the experimental conditions based on the absence of clinical disease, mortality and virus replication. This study showed the potential risks associated with naïve and DGIV-infected fish sharing a common water source.
[Mh] Termos MeSH primário: Peixes-Gato
Infecções por Vírus de DNA/veterinária
Doenças dos Peixes/transmissão
Iridoviridae/fisiologia
Perciformes
[Mh] Termos MeSH secundário: Animais
Austrália
Infecções por Vírus de DNA/transmissão
Infecções por Vírus de DNA/virologia
Suscetibilidade a Doenças/veterinária
Suscetibilidade a Doenças/virologia
Doenças dos Peixes/virologia
Água Doce
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160624
[St] Status:MEDLINE
[do] DOI:10.1111/jfd.12510



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