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[PMID]: 29181623 [Au] Autor: Qiu L; Chen MM; Wang RY; Wan XY; Li C; Zhang QL; Dong X; Yang B; Xiang JH; Huang J [Ad] Endereço: Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Yellow [Ti] Título: Complete genome sequence of shrimp hemocyte iridescent virus (SHIV) isolated from white leg shrimp, Litopenaeus vannamei. [So] Source: Arch Virol;163(3):781-785, 2018 Mar. [Is] ISSN: 1432-8798 [Cp] País de publicação: Austria [La] Idioma: eng [Ab] Resumo: Infection with shrimp hemocyte iridescent virus (SHIV), a new virus of the family Iridoviridae isolated in China, results in a high mortality rate in white leg shrimp (Litopenaeus vannamei). The complete genome sequence of SHIV was determined and analyzed in this study. The genomic DNA was 165,809 bp long with 34.6% G+C content and 170 open reading frames (ORFs). Dotplot analysis showed that the longest repetitive region was 320 bp in length, including 11 repetitions of an 18-bp sequence and 3.1 repetitions of a 39-bp sequence. Two phylogenetic trees were constructed based on 27 or 16 concatenated sequences of proteins encoded by genes that are conserved between SHIV homologous and other iridescent viruses. The results of this study, suggest that SHIV should be considered a member of the proposed new genus "Xiairidovirus". [Mh] Termos MeSH primário: DNA Viral/genética Genoma Viral Iridovirus/genética Penaeidae/virologia Filogenia [Mh] Termos MeSH secundário: Animais Composição de Bases Sequência de Bases Hemócitos/virologia Iridovirus/classificação Iridovirus/isolamento & purificação Fases de Leitura Aberta Análise de Sequência de DNA [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Viral) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180227 [Lr] Data última revisão: 180227 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171129 [St] Status: MEDLINE [do] DOI: 10.1007/s00705-017-3642-4

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[PMID]: 28689858 [Au] Autor: Yu Y; Huang Y; Ni S; Zhou L; Liu J; Zhang J; Zhang X; Hu Y; Huang X; Qin Q [Ad] Endereço: Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China. [Ti] Título: Singapore grouper iridovirus (SGIV) TNFR homolog VP51 functions as a virulence factor via modulating host inflammation response. [So] Source: Virology;511:280-289, 2017 Nov. [Is] ISSN: 1096-0341 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Virus encoded tumor necrosis factor receptor (TNFR) homologues are usually involved in immune evasion by regulating host immune response or cell death. Singapore grouper iridovirus (SGIV) is a novel ranavirus which causes great economic losses in aquaculture industry. Previous studies demonstrated that SGIV VP51, a TNFR-like protein regulated apoptotic process in VP51 overexpression cells. Here, we developed a VP51-deleted recombinant virus Δ51-SGIV by replacing VP51 with puro -GFP. Deletion of VP51 resulted in the decrease of SGIV virulence, evidenced by the reduced replication in vitro and the decreased cumulative mortalities in Δ51-SGIV challenged grouper compared to WT-SGIV. Moreover, VP51 deletion significantly increased virus induced apoptosis, and reduced the expression of pro-inflammatory cytokines in vitro. In addition, the expression of several pro-inflammatory genes were decreased in Δ51-SGIV infected grouper compared to WT-SGIV. Thus, we speculate that SGIV VP51 functions as a critical virulence factor via regulating host cell apoptosis and inflammation response. [Mh] Termos MeSH primário: Interações Hospedeiro-Patógeno Fatores Imunológicos/metabolismo Inflamação/patologia Iridovirus/patogenicidade Receptores do Fator de Necrose Tumoral/metabolismo Proteínas Virais/metabolismo Fatores de Virulência/metabolismo [Mh] Termos MeSH secundário: Animais Apoptose Células Cultivadas Citocinas/secreção Doenças dos Peixes/patologia Doenças dos Peixes/virologia Peixes Deleção de Genes Fatores Imunológicos/genética Iridovirus/genética Iridovirus/fisiologia Receptores do Fator de Necrose Tumoral/genética Análise de Sobrevida Proteínas Virais/genética Virulência Fatores de Virulência/genética Replicação Viral [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cytokines); 0 (Immunologic Factors); 0 (Receptors, Tumor Necrosis Factor); 0 (Viral Proteins); 0 (Virulence Factors) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171010 [Lr] Data última revisão: 171010 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170711 [St] Status: MEDLINE

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[PMID]: 28025159 [Au] Autor: Reshi L; Wang HV; Hui CF; Su YC; Hong JR [Ad] Endereço: Lab of Molecular Virology and Biotechnology, Institute of Biotechnology, National Cheng Kung University, No. 1 University Road, Tainan City 701, Taiwan, ROC; Department of Life Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, No. 1 University Road, Tainan City 701, [Ti] Título: Anti-apoptotic genes Bcl-2 and Bcl-xL overexpression can block iridovirus serine/threonine kinase-induced Bax/mitochondria-mediated cell death in GF-1 cells. [So] Source: Fish Shellfish Immunol;61:120-129, 2017 Feb. [Is] ISSN: 1095-9947 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Although serine/threonine (ST) kinase is known to induce host cell death in GF-1 cells, it remains unclear how ST kinase induces mitochondrial function loss. In the present study, we addressed the issue of mitochondrial function loss by determining whether the Bcl-2 family members Bcl-2 and Bcl-xL can prevent ST kinase-induced cell death activity via interacting with the pro-apoptotic gene Bax. Grouper fin cells (GF-1) carrying EGFP-Bal-xL and EGFP-Bcl-2 fused genes were selected, established in cell culture, and used to examine the involvement of Bcl-2 and Bcl-xL overexpression in protection of GF-1 cells from the effects of the giant sea perch iridovirus (GSIV) ST kinase gene. Using the TUNEL assay, we found that EGFP-Bcl-2 and EGFP-Bcl-xL reduced GSIV ST kinase-induced apoptosis to 20% all at 24 h and 48 h post-transfection (pt). Also, Bcl-2 and Bcl-xL substantially reduced the percentage of cells with GSIV ST kinase-induced loss of mitochondrial membrane potential (Δψps) at 24 and 48 hpt, respectively, and this reduction correlated with a 30% and 50% enhancement of host cell viability at 24 and 48 hpt as compared with vector control. Moreover, analysis of the effect of Bcl-2 and Bcl-xL interaction with Bax targeted to mitochondria during ST kinase expression at 48 hpt found that Bcl-2 and Bcl-xL also interacted with Bax to block cytochrome c release. Finally, Bcl-2 and Bcl-xL overexpression caused blockage of ST kinase function at 48 hpt, which was correlated with preventing caspase-9 and -3 cleavage and activation, thereby blocking downstream death signaling events. Taken together, our results suggest that the ST kinase-induced Bax/mitochondria-mediated cell death pathway can be blocked by the interaction of Bcl-2 and Bcl-xL with Bax to inhibit cytochrome c release during MMP loss. This rescue activity also correlated with inhibition of caspase-9 and -3 activation, thereby enhancing cell viability. [Mh] Termos MeSH primário: Bass/genética Proteínas de Peixes/genética Iridovirus/fisiologia Proteínas Serina-Treonina Quinases/genética Proteínas Proto-Oncogênicas c-bcl-2/genética Proteína X Associada a bcl-2/genética [Mh] Termos MeSH secundário: Animais Bass/metabolismo Bass/virologia Linhagem Celular Proteínas de Peixes/metabolismo Potencial da Membrana Mitocondrial Mitocôndrias/metabolismo Proteínas Serina-Treonina Quinases/metabolismo Proteínas Proto-Oncogênicas c-bcl-2/metabolismo Transdução de Sinais Proteína X Associada a bcl-2/metabolismo Proteína bcl-X/genética Proteína bcl-X/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fish Proteins); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (bcl-2-Associated X Protein); 0 (bcl-X Protein); EC 2.7.11.1 (Protein-Serine-Threonine Kinases) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170410 [Lr] Data última revisão: 170410 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161228 [St] Status: MEDLINE

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[PMID]: 27786157 [Au] Autor: Yu XB; Chen XH; Shan LP; Hao K; Wang GX [Ad] Endereço: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China. [Ti] Título: In vitro antiviral efficacy of moroxydine hydrochloride and ribavirin against grass carp reovirus and giant salamander iridovirus. [So] Source: Dis Aquat Organ;121(3):189-199, 2016 10 27. [Is] ISSN: 0177-5103 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Moroxydine hydrochloride (Mor) and ribavirin (Rib) have been reported to exhibit multi-antiviral activities against DNA and RNA viruses, but their antiviral activities and pharmacologies have seldom been studied in aquaculture. This paper has selected 3 aquatic viruses including a double-stranded RNA virus (grass carp reovirus, GCRV), a single-stranded RNA virus (spring viraemia of carp virus, SVCV) and a DNA virus (giant salamander iridovirus, GSIV) for antiviral testing. The results showed that Mor and Rib can effectively control the infection of GCRV and GSIV in respective host cells. Further study was undertaken to explore the antivirus efficiencies and pharmacological mechanisms of Mor and Rib on GCRV and GSIV in vitro. Briefly, compounds showed over 50% protective effects at 15.9 µg ml-1 except for the group of GSIV-infected epithelioma papulosum cyprinid (EPC) cells treated with Mor. Moreover, Mor and Rib blocked the virus-induced cytopathic effects and apoptosis in host cells to keep the normal cellular structure. The expression of VP1 (GCRV) and major capsid protein (MCP; GSIV) gene was also significantly inhibited in the virus-infected cells when treated with Mor and Rib. Cytotoxicity assay verified the 2 compounds had no toxic effects on grass carp ovary (GCO) cells and EPC cells at ≤96 µg ml-1. In conclusion, these results indicated that exposing GCRV-infected GCO cells and GSIV-infected EPC cells to Mor and Rib could elicit significant antiviral responses, and the 2 compounds have been shown to be promising agents for viral control in the aquaculture industry. [Mh] Termos MeSH primário: Antivirais/farmacologia Iridovirus/efeitos dos fármacos Morfolinas/farmacologia Reoviridae/efeitos dos fármacos Ribavirina/farmacologia [Mh] Termos MeSH secundário: Animais Antivirais/administração & dosagem Linhagem Celular Sobrevivência Celular Relação Dose-Resposta a Droga Peixes Morfolinas/administração & dosagem Ribavirina/administração & dosagem [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Antiviral Agents); 0 (Morpholines); 49717AWG6K (Ribavirin); O611591WAH (moroxydine) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170622 [Lr] Data última revisão: 170622 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161028 [St] Status: MEDLINE

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[PMID]: 27688178 [Au] Autor: Oh SY; Nishizawa T [Ad] Endereço: Department of Aqualife Medicine, Chonnam National University, Yeosu 59626, Republic of Korea. [Ti] Título: Establishment of rock bream Oplegnathus fasciatus embryo (RoBE-4) cells with cytolytic infection of red seabream iridovirus (RSIV). [So] Source: J Virol Methods;238:1-5, 2016 Dec. [Is] ISSN: 1879-0984 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Red seabream iridovirus (RSIV) is a member of genus Megalocytivirus in the family Iridoviridae. RSIV infection causes significant economic losses of marine-fishes in East Asian countries. Grunt fin (GF) cell line has been commonly used for culturing RSIV. However, it is not suitable for definite evaluation of infectivity titer of RSIV because cells infected with RSIV are not completely cytolysed. Thus, we established a new cell line, RoBE-4, from rock bream (Oplegnathus fasciatus) eyed-egg embryos in this study. Morphologically, RoBE-4 cells were fibroblastic-like. They have been stably grown over two-years with 60 passages using Leibovitz's L-15 medium containing 10% (v/v) fetal bovine serum. RoBE-4 cells infected with RSIV exhibited cytopathic effects (CPE) with cell rounding. They were cytolysed completely after ≥2 weeks of culture. Numerous RSIV particles with icosahedral morphology of approximately 122nm in diameter were observed in cytoplasmic area of infected RoBE-4 cells. The RSIV-suceptibility and amount of extracellular RSIV released by RoBE-4 cells were 100-fold higher than those by GF cells. RSIV cultured with RoBE-4 cells was highly virulent to rock bream in infection experiments. Therefore, using RoBE-4 cells instead of GF cells will enable accurate and sensitive measurement of RSIV infectivity. In addition, RoBE-4 cells might be used to produce RSIV vaccine in the future with significant reduction in cost. [Mh] Termos MeSH primário: Linhagem Celular Embrião não Mamífero Iridovirus/isolamento & purificação Iridovirus/fisiologia Dourada [Mh] Termos MeSH secundário: Animais Técnicas de Cultura de Células Morte Celular Efeito Citopatogênico Viral Embrião não Mamífero/citologia Embrião não Mamífero/virologia Iridovirus/química Iridovirus/crescimento & desenvolvimento Dourada/embriologia Dourada/virologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171013 [Lr] Data última revisão: 171013 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161001 [St] Status: MEDLINE

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[PMID]: 27667470 [Au] Autor: Zhou X; Zhang X; Jia Q; Han Y; Gao H [Ad] Endereço: Aquaculture and Fishery Engineering Laboratory, Yellow River Fisheries Institute, Chinese Academy of Fishery Sciences, Xi'an 710086, China. [Ti] Título: [Prokaryotic expression and antiserum preparation for major antigenic epitope region of major capsid protein of Chinese giant salamander (Andrias davidianus) iridovirus]. [So] Source: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi;32(10):1407-1411, 2016 Oct. [Is] ISSN: 1007-8738 [Cp] País de publicação: China [La] Idioma: chi [Ab] Resumo: Objective To express the fusion protein of major antigenic epitope region of major capsid protein (MCP) of Chinese giant salamander (Andrias davidianus) iridovirus (CGSIV) and prepare the rabbit antiserum. Methods Using the genomic DNA of CGSIV Lueyang strain (CGSIV-LY) as a template, the gene fragment of major antigenic epitope region of MCP was amplified by PCR and cloned into the prokaryotic vector pET-21a(+) to construct the prokaryotic expression recombinant plasmid pET-21a-MCP. The recombinant plasmid was transformed into Escherichia coli BL21(DE3). His-tagged fusion protein was induced by IPTG. After identified by SDS-PAGE and Western blot analysis, the recombinant protein was purified by nitrilotriacetic acid (Ni-NTA) agarose resin. New Zealand rabbits were immunized with the purified recombinant protein to generate antiserum. Specificity and titer of the antiserum were determined by Western blotting and indirect ELISA, and then the antiserum was used to detect the CGSIV in the infected EPC cells by indirect immunofluorescence assay. Results The recombinant protein with the relative molecular mass of 29 000 was expressed. The prepared rabbit antiserum had a good specificity and a high titer. Indirect immunofluorescence assay showed that the antiserum could recognize CGSIV in the infected EPC cells. Conclusion The fusion protein of major antigenic epitope region of MCP of CGSIV is successfully expressed and the rabbit antiserum with a high titer and a good specificity been prepared. [Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia Proteínas do Capsídeo/genética Escherichia coli/genética Soros Imunes/imunologia Iridovirus/imunologia [Mh] Termos MeSH secundário: Animais Especificidade de Anticorpos Proteínas do Capsídeo/imunologia Clonagem Molecular Escherichia coli/metabolismo Expressão Gênica Iridovirus/genética Iridovirus/isolamento & purificação Coelhos Proteínas Recombinantes/genética Proteínas Recombinantes/imunologia Urodelos/virologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antibodies, Viral); 0 (Capsid Proteins); 0 (Immune Sera); 0 (Recombinant Proteins) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170629 [Lr] Data última revisão: 170629 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160927 [St] Status: MEDLINE

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[PMID]: 27496102 [Au] Autor: Dizman YA; Muratoglu H; Sandalli C; Nalcacioglu R; Demirbag Z [Ad] Endereço: Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey. [Ti] Título: Chilo iridescent virus (CIV) ORF 012L encodes a protein with both exonuclease and endonuclease functions. [So] Source: Arch Virol;161(11):3029-37, 2016 Nov. [Is] ISSN: 1432-8798 [Cp] País de publicação: Austria [La] Idioma: eng [Ab] Resumo: Chilo iridescent virus (CIV) is the type member of the genus Iridovirus within the family Iridoviridae. The virions of CIV contain a single linear dsDNA molecule that is circularly permuted and terminally redundant. The genome of CIV contains an open reading frame (ORF 012L) encoding a protein homologous to exonuclease II of Schizosaccharomyces pombe. In this study, we focused on the characterization of CIV ORF 012L. The target ORF was cloned into the pET28a vector, expressed in E. coli strain BL21 (DE3) pLysS with an N-terminal His tag and purified to homogeneity by using Ni-NTA affinity chromatography. Biochemical characterization of the purified CIV 012L confirmed that this viral protein is a functional 5'-3' exonuclease that digests 3'-biotin-labelled oligonucleotides and linear double-stranded DNA (dsDNA) molecules from their 5' termini in a highly processive manner. CIV 012L also has a potent endonuclease activity on dsDNA in vitro. In addition, CIV 012L converted supercoiled plasmid DNA (replicative form I, RFI) into the open circular form (RFII) and then open circular form into linear form (RFIII). Endonuclease activity of CIV 012L was optimal in the presence of 10 mM Mg(2+) or 30 mM Mn(2+) ions and at 150 mM NaCl or KCl salt concentrations. The highest endonuclease activity was obtained at pH 8, and it reached a maximum at 55 °C. The CIV 012L protein showed deficiencies for both double- and single-stranded RNAs. [Mh] Termos MeSH primário: Endonucleases/metabolismo Exonucleases/metabolismo Iridovirus/enzimologia Proteínas Virais/metabolismo [Mh] Termos MeSH secundário: Cromatografia de Afinidade Clonagem Molecular DNA/metabolismo DNA Circular/metabolismo Endonucleases/química Endonucleases/genética Ativadores de Enzimas/análise Estabilidade Enzimática Escherichia coli/genética Escherichia coli/metabolismo Exonucleases/química Exonucleases/genética Expressão Gênica Concentração de Íons de Hidrogênio Iridovirus/genética Fases de Leitura Aberta Proteínas Recombinantes/química Proteínas Recombinantes/genética Proteínas Recombinantes/isolamento & purificação Proteínas Recombinantes/metabolismo Temperatura Ambiente Proteínas Virais/química Proteínas Virais/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Circular); 0 (Enzyme Activators); 0 (Recombinant Proteins); 0 (Viral Proteins); 9007-49-2 (DNA); EC 3.1.- (Endonucleases); EC 3.1.- (Exonucleases) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170126 [Lr] Data última revisão: 170126 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160807 [St] Status: MEDLINE [do] DOI: 10.1007/s00705-016-3007-4

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[PMID]: 27449679 [Au] Autor: Vávra J; Bílý T; Nebesárová J; Federici BA [Ad] Endereço: Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovská 31, CZ-37005 Ceské Budejovice, Czech Republic; Faculty of Science, University of South Bohemia, Branisovská 1760, CZ-37005 Ceské Budejovice, Czech Republic; Faculty of Science, Charles University in Prague, Vinic [Ti] Título: Occurrence, pathology, and ultrastructure of iridovirus and cytoplasmic polyhedrosis viruses in daphnids from the Czech Republic. [So] Source: J Invertebr Pathol;140:35-38, 2016 Oct. [Is] ISSN: 1096-0805 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Iridescent (IVs, family Iridoviridae, genus Iridovirus) and cytoplasmic polyhedrosis viruses (CPVs; family Reoviridae, genus Cypovirus) are well known in insects, with thirteen IV species recognized from various orders, and sixteen CPV species known from lepidopterans. In 1975, an IV and CPV were reported in the daphnid, Simocehpalus expinosus, in Florida, but other reported daphnid virus infections seem to be rare. Here we report infected daphnids from woodland and carp ponds in the Czech Republic, Daphnia curvirostris with an IV, and D. pulex and D. ambigua, with CPVs. This suggests these viruses are more common in daphnids, the rarity of reports due to few surveys. [Mh] Termos MeSH primário: Daphnia/virologia Viroses/veterinária [Mh] Termos MeSH secundário: Animais República Tcheca Iridovirus Microscopia de Força Atômica Microscopia Eletrônica de Transmissão Reoviridae [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170926 [Lr] Data última revisão: 170926 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160725 [St] Status: MEDLINE

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[PMID]: 27422159 [Au] Autor: Guo M; Wei J; Zhou Y; Qin Q [Ad] Endereço: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China; Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzho [Ti] Título: c-Jun N-terminal kinases 3 (JNK3) from orange-spotted grouper, Epinephelus coioides, inhibiting the replication of Singapore grouper iridovirus (SGIV) and SGIV-induced apoptosis. [So] Source: Dev Comp Immunol;65:169-181, 2016 12. [Is] ISSN: 1879-0089 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: C-Jun N-terminal kinases (JNKs), a subgroup of serine-threonine protein kinases that activated by phosphorylation, are involve in physiological and pathophysiological processes. JNK3 is one of JNK proteins involved in JNK3 signaling transduction. In the present study, two JNK3 isoforms, Ec-JNK3 X1 and Ec-JNK3 X2, were cloned from orange-spotted grouper, Epinephelus coioides. Both Ec-JNK3 X1 and Ec-JNK3 X2 were mainly expressed in liver, gill, skin, brain and muscle of juvenile grouper. The relative expression of Ec-JNK3 X2 mRNA was much higher in muscle and gill than that of Ec-JNK3 X1. Isoform-specific immune response to challenges was revealed by the expression profiles in vivo. Immunofluorescence staining indicated that JNK3 was localized in the cytoplasm of grouper spleen (GS) cells and shown immune response to SGIV infection in vitro. Over-expressing Ec-JNK3 X1 and/or Ec-JNK3 X2 inhibited the SGIV infection and replication and the SGIV-induced apoptosis. To achieve the antiviral and anti-apoptosis activities, JNK3 promoted the activation of genes ISRE and type I IFN in the antiviral IFN signaling pathway, and inhibited the activation of transcription factors NF-κB and p53 relating to apoptosis, respectively. Ec-JNK3 X2 showed stronger activities in antivirus and anti-apoptosis than that of Ec-JNK3 X1. Our results not only define the characterization of JNK3 but also reveal new immune functions and the molecular mechanisms of JNK3 on iridoviruses infection and the virus-induced apoptosis. [Mh] Termos MeSH primário: Infecções por Vírus de DNA/imunologia Doenças dos Peixes/imunologia Proteínas de Peixes/metabolismo Iridovirus/fisiologia Proteína Quinase 10 Ativada por Mitógeno/metabolismo Perciformes/imunologia [Mh] Termos MeSH secundário: Animais Apoptose Células Cultivadas Clonagem Molecular Proteínas de Peixes/genética Especificidade de Hospedeiro Interferon Tipo I/genética Interferon Tipo I/metabolismo Proteína Quinase 10 Ativada por Mitógeno/genética Isoformas de Proteínas/genética Transdução de Sinais Replicação Viral [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Fish Proteins); 0 (Interferon Type I); 0 (Protein Isoforms); EC 2.7.1.- (Mitogen-Activated Protein Kinase 10) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171120 [Lr] Data última revisão: 171120 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160717 [St] Status: MEDLINE

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[PMID]: 27369385 [Au] Autor: Nalcacioglu R; Muratoglu H; Yesilyurt A; van Oers MM; Vlak JM; Demirbag Z [Ad] Endereço: Karadeniz Technical University, Faculty of Science, Department of Biology, 61080 Trabzon, Turkey. Electronic address: remziye@ktu.edu.tr. [Ti] Título: Enhanced insecticidal activity of Chilo iridescent virus expressing an insect specific neurotoxin. [So] Source: J Invertebr Pathol;138:104-11, 2016 Jul. [Is] ISSN: 1096-0805 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Previously we have generated a recombinant Chilo iridescent virus (CIV) by inserting the green fluorescent protein gene (gfp) into the CIV 157L open reading frame (ORF) locus and showed that this recombinant (rCIV-Δ157L-gfp) was fully infectious both in cell culture as well as in insect larvae. This study opened up a new avenue for increasing the speed of kill of CIV and other iridoviruses by inserting virulence or toxin genes into the viral genome. In the current study we constructed a recombinant CIV (rCIV-Δ157L/gfp-AaIT) where the 157L ORF was replaced with both the AaIT neurotoxin gene from the scorpion Androctonus australis and the gfp gene, each under control of the viral major capsid protein (mcp) gene promoter. Recombinant virus was purified by successive rounds of plaque purification using Spodoptera frugiperda (Sf-9) cells. One-step growth curves for the recombinant viruses, rCIV-Δ157L/gfp-AaIT and rCIV-Δ157L-gfp, and wild-type CIVs in Sf-9 cells showed similar profiles. AaIT toxin expression in infected third instar Galleria mellonella larvae was confirmed by western blot analysis using an antibody against the AaIT protein. rCIV-Δ157L/gfp-AaIT infection at a concentration that kills 100% of the larvae caused paralysis in infected third instar G. mellonella larvae from two days after injection, whereas infection with non-AaIT containing viruses showed mortality starting much later (>10days). Bioassays on these larvae demonstrated that the speed of kill of CIV carrying AaIT was strikingly enhanced as compared to wild-type CIV. These results suggest that insertion of a toxin gene into CIV provides further opportunities to control a wide range of pest insects, such as weevils, using an iridovirus. [Mh] Termos MeSH primário: Inseticidas Iridovirus/genética Mariposas/virologia Controle Biológico de Vetores/métodos Venenos de Escorpião/genética [Mh] Termos MeSH secundário: Animais Western Blotting Engenharia Genética Vetores Genéticos Reação em Cadeia da Polimerase [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Insecticides); 0 (Scorpion Venoms); 131092-86-9 (AaIT neurotoxin, Androctonus australis) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170915 [Lr] Data última revisão: 170915 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160703 [St] Status: MEDLINE

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