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  1 / 1540 MEDLINE  
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[PMID]:27473724
[Au] Autor:Yang S; Liu Z; Wang Y; Li W; Fu X; Lin Y; Shen Q; Wang X; Wang H; Zhang W
[Ad] Endereço:School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of China.
[Ti] Título:A novel rodent Chapparvovirus in feces of wild rats.
[So] Source:Virol J;13:133, 2016 Jul 29.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chapparvovirus, a recently determined new genus in the family Parvoviridae, can infect many species of animals including bats, chickens, and pigs. Here, using viral metagenomics method, we identified a novel Chapparvovirus from feces of wild rats and designated it as rat parvovirus 2 (RPV2). The nearly complete genome of RPV2 is 4222-nt long and includes two ORFs encoding a 654-aa nonstructural protein 1 (NS1) and a 472-aa capsid protein (VP), respectively. Phylogenetic analysis over the amino acid sequence of the NS1 showed that RPV2 clustered with Eidolon helvum parvovirus 2 (EHPV2), porcine parvovirus 7 (PPV7), and turkey parvovirus 1 (TP1), forming a separate clade. Sequence analysis indicated that the NS1 protein of RPV2 shared the highest amino acid sequence identity (51 %) with that of EHPV2. According to the genetic distance-based criteria, RPV2 identified here belongs to a novel species of Chapparvovirus.
[Mh] Termos MeSH primário: Infecções por Parvoviridae/veterinária
Parvoviridae/isolamento & purificação
Doenças dos Roedores/virologia
[Mh] Termos MeSH secundário: Animais
Animais Selvagens/virologia
Galinhas
China
Fezes/virologia
Genoma Viral
Fases de Leitura Aberta
Parvoviridae/classificação
Parvoviridae/genética
Parvoviridae/metabolismo
Infecções por Parvoviridae/virologia
Filogenia
Ratos
Suínos
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170518
[Lr] Data última revisão:
170518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160731
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-016-0589-0


  2 / 1540 MEDLINE  
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Roehe, Paulo Michel
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[PMID]:26986573
[Au] Autor:Kluge M; Campos FS; Tavares M; de Amorim DB; Valdez FP; Giongo A; Roehe PM; Franco AC
[Ad] Endereço:Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, UFRGS (Federal University of Rio Grande do Sul), Porto Alegre, Rio Grande do Sul, Brazil.
[Ti] Título:Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals.
[So] Source:PLoS One;11(3):e0151921, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Brazilian South coast seasonally hosts numerous marine species, observed particularly during winter months. Some animals, including fur seals, are found dead or debilitated along the shore and may harbor potential pathogens within their microbiota. In the present study, a metagenomic approach was performed to evaluate the viral diversity in feces of fur seals found deceased along the coast of the state of Rio Grande do Sul. The fecal virome of two fur seal species was characterized: the South American fur seal (Arctocephalus australis) and the Subantarctic fur seal (Arctocephalus tropicalis). Fecal samples from 10 specimens (A. australis, n = 5; A. tropicalis, n = 5) were collected and viral particles were purified, extracted and amplified with a random PCR. The products were sequenced through Ion Torrent and Illumina platforms and assembled reads were submitted to BLASTx searches. Both viromes were dominated by bacteriophages and included a number of potentially novel virus genomes. Sequences of picobirnaviruses, picornaviruses and a hepevirus-like were identified in A. australis. A rotavirus related to group C, a novel member of the Sakobuvirus and a sapovirus very similar to California sea lion sapovirus 1 were found in A. tropicalis. Additionally, sequences of members of the Anelloviridae and Parvoviridae families were detected in both fur seal species. This is the first metagenomic study to screen the fecal virome of fur seals, contributing to a better understanding of the complexity of the viral community present in the intestinal microbiota of these animals.
[Mh] Termos MeSH primário: Otárias/virologia
Metagenômica
Vírus/genética
[Mh] Termos MeSH secundário: Anelloviridae/genética
Animais
Bacteriófagos/genética
Sequência de Bases
Fezes/virologia
Genoma Viral/genética
Biblioteca Genômica
Herpesviridae/genética
Dados de Sequência Molecular
Parvoviridae/genética
Filogenia
Picobirnavirus/genética
Picornaviridae/genética
Rotavirus/genética
Sapovirus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160318
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0151921


  3 / 1540 MEDLINE  
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[PMID]:26911883
[Au] Autor:Sánchez-Rodríguez SP; Morán-García Adel C; Bolonduro O; Dordick JS; Bustos-Jaimes I
[Ad] Endereço:Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA; Department of Biochemistry, Faculty of Medicine, National Autonomous University of Mexico, Mexico City 04510, Mexico.
[Ti] Título:Enhanced assembly and colloidal stabilization of primate erythroparvovirus 1 virus-like particles for improved surface engineering.
[So] Source:Acta Biomater;35:206-14, 2016 Apr 15.
[Is] ISSN:1878-7568
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Virus-like particles (VLPs) are the product of the self-assembly, either in vivo or in vitro, of structural components of viral capsids. These particles are excellent scaffolds for surface display of biomolecules that can be used in vaccine development and tissue-specific drug delivery. Surface engineering of VLPs requires structural stability and chemical reactivity. Herein, we report the enhanced assembly, colloidal stabilization and fluorescent labeling of primate erythroparvovirus 1 (PE1V), generally referred to as parvovirus B19. In vitro assembly of the VP2 protein of PE1V produces VLPs, which are prone to flocculate and hence undergo limited chemical modification by thiol-specific reagents like the fluorogenic monobromobimane (mBBr). We determined that the addition of 0.2M l-arginine during the assembly process produced an increased yield of soluble VLPs with good dispersion stability. Fluorescent labeling of VLPs suspended in phosphate buffered saline (PBS) added with 0.2M l-Arg was achieved in significantly shorter times than the flocculated VLPs assembled in only PBS buffer. Finally, to demonstrate the potential application of this approach, mBBr-labeled VLPs were successfully used to tag human hepatoma HepG2 cells. This new method for assembly and labeling PE1V VLPs eases its applications and provides insights on the manipulation of this biomaterial for further developments. STATEMENT OF SIGNIFICANCE: Application of virus-derived biomaterials sometimes requires surface modification for diverse purposes, including enhanced cell-specific interaction, the inclusion of luminescent probes for bioimaging, or the incorporation of catalytic properties for the production of enzyme nanocarriers. In this research, we reported for the first time the colloidal stabilization of the primate erythroparvovirus 1 (PE1V) virus-like particles (VLPs). Also, we report the chemical modification of the natural Cys residues located on the surface of these VLPs with a fluorescent probe, as well as its application for tagging hepatoma cells in vitro. Keeping in mind that PE1V is a human pathogen, virus-host interactions already exist in human cells, and they can be exploited for therapeutic and research aims. This study will impact on the speed in which the scientific community will be able to manipulate PE1V VLPs for diverse purposes. Additionally, this study may provide insights on the colloidal properties of these VLPs as well as in the effect of different protein additives used for protein stabilization.
[Mh] Termos MeSH primário: Coloides/química
Parvoviridae/química
Engenharia de Proteínas/métodos
Vírion/química
[Mh] Termos MeSH secundário: Animais
Arginina/farmacologia
Compostos Bicíclicos com Pontes/metabolismo
Centrifugação
Cristalografia por Raios X
Endocitose
Filtração
Fluorescência
Glicerol/farmacologia
Células Hep G2
Seres Humanos
Concentração de Íons de Hidrogênio
Modelos Moleculares
Primatas
Compostos de Sulfidrila/metabolismo
Propriedades de Superfície
Proteínas Virais/química
Proteínas Virais/metabolismo
Vírion/efeitos dos fármacos
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bridged Bicyclo Compounds); 0 (Colloids); 0 (Sulfhydryl Compounds); 0 (Viral Proteins); 94ZLA3W45F (Arginine); PDC6A3C0OX (Glycerol); V23UK0CYXL (monobromobimane)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160226
[St] Status:MEDLINE


  4 / 1540 MEDLINE  
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[PMID]:25382194
[Au] Autor:Filipov C; Desario C; Patouchas O; Eftimov P; Gruichev G; Manov V; Filipov G; Buonavoglia C; Decaro N
[Ad] Endereço:Faculty of Veterinary Medicine, University of Forestry, Sofia, Bulgaria.
[Ti] Título:A Ten-Year Molecular Survey on Parvoviruses Infecting Carnivores in Bulgaria.
[So] Source:Transbound Emerg Dis;63(4):460-4, 2016 Aug.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Parvoviruses represent the most important infectious agents that are responsible for severe to fatal disease in carnivores. This study reports the results of a 10-year molecular survey conducted on carnivores in Bulgaria (n = 344), including 262 dogs and 19 cats with gastroenteritis, and 57 hunted wild carnivores. Real-time polymerase chain reaction (qPCR), followed by virus characterization by minor groove binder (MGB) probe assays, detected 216 parvovirus positive dogs with a predominance of canine parvovirus type 2a (CPV-2a, 79.17%) over CPV-2b (18.52%) and CPV-2c (2.31%). Rottweilers and German shepherds were the most frequent breeds among CPV-positive pedigree dogs (n = 96). Eighteen cats were found to shed parvoviruses in their faeces, with most strains being characterized as FPLV (n = 17), although a single specimen tested positive for CPV-2a. Only two wild carnivores were parvovirus positive, a wolf (Canis lupus) and a red fox (Vulpes vulpes), both being infected by CPV-2a strains.
[Mh] Termos MeSH primário: Carnívoros/virologia
Infecções por Parvoviridae/epidemiologia
Infecções por Parvoviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Bulgária/epidemiologia
Gatos
Cães
Fezes/virologia
Parvoviridae/classificação
Parvoviridae/genética
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141111
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12285


  5 / 1540 MEDLINE  
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[PMID]:26383859
[Au] Autor:Lanave G; Martella V; Farkas SL; Marton S; Fehér E; Bodnar L; Lavazza A; Decaro N; Buonavoglia C; Bányai K
[Ad] Endereço:Department of Veterinary Medicine, University Aldo Moro of Bari, Km 3 St. for Casamassima, Valenzano 70010, Italy.
[Ti] Título:Novel bocaparvoviruses in rabbits.
[So] Source:Vet J;206(2):131-5, 2015 Nov.
[Is] ISSN:1532-2971
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bocaparvovirus is a newly established genus within the family Parvoviridae and has been identified as a possible cause of enteric, respiratory, reproductive/neonatal and neurological disease in humans and several animal species. In this study, metagenomic analysis was used to identify and characterise a novel bocaparvovirus in the faeces of rabbits with enteric disease. To assess the prevalence of the novel virus, rectal swabs and faecal samples obtained from rabbits with and without diarrhoea were screened with a specific PCR assay. The complete genome sequence of the novel parvovirus was reconstructed. The virus was distantly related to other bocaparvoviruses; the three ORFs shared 53%, 53% and 50% nucleotide identity, respectively, to homologous genes of porcine bocaparvoviruses. The virus was detected in 8/29 (28%) and 16/95 (17%) samples of rabbits with and without diarrhoea, respectively. Sequencing of the capsid protein fragment targeted by the diagnostic PCR identified two distinct bocaparvovirus populations/sub-types, with 91.7-94.5% nucleotide identity to each other. Including these novel parvoviruses in diagnostic algorithms of rabbit diseases might help inform their potential pathogenic role and impact on rabbit production and the virological profiles of laboratory rabbits.
[Mh] Termos MeSH primário: Infecções por Parvoviridae/veterinária
Parvoviridae
Coelhos
[Mh] Termos MeSH secundário: Animais
Genoma Viral
Parvoviridae/genética
Infecções por Parvoviridae/virologia
Filogenia
Cultura de Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150919
[St] Status:MEDLINE


  6 / 1540 MEDLINE  
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[PMID]:26239432
[Au] Autor:Tu M; Liu F; Chen S; Wang M; Cheng A
[Ad] Endereço:Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang District, Chengdu City, Sichuan Province, 611130, China. ttiyun@163.com.
[Ti] Título:Role of capsid proteins in parvoviruses infection.
[So] Source:Virol J;12:114, 2015 Aug 04.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The parvoviruses are widely spread in many species and are among the smallest DNA animal viruses. The parvovirus is composed of a single strand molecule of DNA wrapped into an icosahedral capsid. In a viral infection, the massy capsid participates in the entire viral infection process, which is summarized in this review. The capsid protein VP1 is primarily responsible for the infectivity of the virus, and the nuclear localization signal (NLS) of the VP1 serves as a guide to assist the viral genome in locating the nucleus. The dominant protein VP2 provides an "anti-receptor", which interacts with the cellular receptor and leads to the further internalization of virus, and, the N-terminal of VP2 also cooperates with the VP1 to prompt the process of nucleus translocation. Additionally, a cleavage protein VP3 is a part of the capsid, which exists only in several members of the parvovirus family; however, the function of this cleavage protein remains to be fully determined. Parvoviruses can suffer from the extreme environmental conditions such as low pH, or even escape from the recognition of pattern recognition receptors (PRRs), due to the protection of the stable capsid, which is thought to be an immune escape mechanism. The applications of the capsid proteins to the screening and the treatment of diseases are also discussed. The processes of viral infection should be noted, because understanding the virus-host interactions will contribute to the development of therapeutic vaccines.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/metabolismo
Infecções por Parvoviridae/virologia
Parvoviridae/metabolismo
[Mh] Termos MeSH secundário: Animais
Capsídeo/metabolismo
Proteínas do Capsídeo/genética
Núcleo Celular/metabolismo
Genoma Viral
Seres Humanos
Fases de Leitura Aberta
Parvoviridae/genética
Transporte Proteico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150805
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-015-0344-y


  7 / 1540 MEDLINE  
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[PMID]:26239342
[Au] Autor:Shen H; Zhang W; Wang H; Zhou Y; Shao S
[Ad] Endereço:Medical College, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, People's Republic of China. hxshen@ujs.edu.cn.
[Ti] Título:Identification of recombination between Muscovy duck parvovirus and goose parvovirus structural protein genes.
[So] Source:Arch Virol;160(10):2617-21, 2015 Oct.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Waterfowl parvoviruses are divided into Muscovy duck parvoviruses (MDPVs) and goose parvoviruses (GPVs). Phylogenetic analysis based on structural gene nucleotide sequences showed that the strains of three GPVs (DY, PT and D strains) and two MDPVs (GX5 and SAAH-SHNH) are closely related and formed one cluster. Recombination analysis showed that recombination between GPV-GDFsh and MDPV-89384/FRANCE strains led to five recombinant strains: GPV-DY, GPV-PT, GPV-D, MDPV-GX5 and MDPV-SAAH-SHNH. The recombinant event was confirmed using the Simplot program and phylogenetic analysis. This is the first comprehensive investigation of recombination between MDPV and GPV structural genes.
[Mh] Termos MeSH primário: Infecções por Parvoviridae/veterinária
Parvoviridae/genética
Doenças das Aves Domésticas/virologia
Recombinação Genética
Proteínas Estruturais Virais/genética
[Mh] Termos MeSH secundário: Animais
Patos
França
Gansos
Dados de Sequência Molecular
Parvoviridae/classificação
Parvoviridae/isolamento & purificação
Infecções por Parvoviridae/virologia
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Structural Proteins)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150926
[Lr] Data última revisão:
150926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150805
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-015-2541-9


  8 / 1540 MEDLINE  
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[PMID]:26085285
[Au] Autor:Wang S; Cheng X; Chen S; Lin F; Chen S; Zhu X; Wang J
[Ad] Endereço:Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, 247 Wusi Road, Fuzhou, 350003, Fujian, People's Republic of China.
[Ti] Título:Evidence for natural recombination in the capsid gene VP2 of Taiwanese goose parvovirus.
[So] Source:Arch Virol;160(8):2111-5, 2015 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:To investigate the possible role of recombination in the evolution of Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) in Taiwan, we analyzed a potentially significant recombination event that occurred only in GPV by comparing thirteen complete sequences of the capsid gene VP2 of GPV and MDPV. The recombination event occurred between GPV strain 06-0239 as the minor parent and strains 99-0808 as the major parent, which resulted in the GPV recombinant V325/TW03. GPV V325/TW03 is likely to represent a new genotype among the Taiwanese GPV strains. This represents the first evidence that intergenotype recombination within the VP2 gene cluster contributes to the genetic diversity of the VP2 genes of Taiwanese GPV field strains.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/genética
Gansos/virologia
Infecções por Parvoviridae/veterinária
Parvoviridae/genética
Doenças das Aves Domésticas/virologia
Recombinação Genética
[Mh] Termos MeSH secundário: Animais
Dados de Sequência Molecular
Parvoviridae/classificação
Parvoviridae/isolamento & purificação
Infecções por Parvoviridae/virologia
Filogenia
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150812
[Lr] Data última revisão:
150812
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150619
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-015-2491-2


  9 / 1540 MEDLINE  
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[PMID]:25968106
[Au] Autor:Knibb W; Quinn J; Kuballa A; Powell D; Remilton C; Nguyen NH
[Ad] Endereço:The University of the Sunshine Coast, Maroochydore, QLD 4558, Australia. Electronic address: wknibb@usc.edu.au.
[Ti] Título:Yearly, pond, lineage and family variation of hepatopancreatic parvo-like virus (HPV) copy number in banana shrimp Fenneropenaeus merguiensis.
[So] Source:J Invertebr Pathol;128:73-9, 2015 Jun.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hepatopancreatic parvo-like virus (HPV) has been reported from a variety of shrimp species around the world, including Australia, and thought to impact negatively on production, but until now there was scant information available on variation of HPV over time, ponds and shrimp lineages or families, information that could be used to manage or reduce virus levels. Here we report HPV copy number estimated using qPCR from 1500 individual shrimp sampled over three years and encompassing 91 ponds, 21 breeding groups or lineages and 40 families. HPV copy number variation between ponds was used by farm management as a criterion to choose prospective broodstock (candidates were taken from low HPV ponds). Despite such choice, HPV levels in farmed animals were not reduced from 2011 to 2013. Accordingly, the hypothesis that HPV levels can be reduced over time simply by considering average HPV levels in ponds alone is rejected. Different lines of shrimp within the same farm had different HPV levels, but as lines were raised separately, the line differences could be due to either genetic or environmental differences, the latter including possible different rearing effects and differences in vertical transmission. There were large (up to 2-3 LOG fold) differences of HPV levels between families bred and grown together contemporaneously, and the heritability for HPV copy number was estimated to be moderate to large (0.40 ± 0.13). Apart from genetic differences, differences of vertical transmission from dams may contribute to the between family differences, in any case we postulate that selection between families could be an effective method to reduce HPV levels. HPV levels were not genetically correlated with performance traits such as body weight or length, so selection for HPV level should not adversely affect production characteristics. This is the first evidence for an aquacultured species that viral levels, as opposed to survival/resistance to viruses, may have a substantial host genetic component. The heritability reported here for virus copy number was higher that most heritabilities reported for survival to specific pathogens such as white spot, raising the general postulate that selection for virus copy number may be more effective and repeatable than selection for survival to pathogen challenge.
[Mh] Termos MeSH primário: Parvoviridae
Penaeidae/virologia
Tanques/microbiologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Animais
Aquicultura/normas
Reação em Cadeia da Polimerase
Frutos do Mar/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150514
[St] Status:MEDLINE


  10 / 1540 MEDLINE  
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[PMID]:25951971
[Au] Autor:Chieochansin T; Vutithanachot V; Theamboonlers A; Poovorawan Y
[Ad] Endereço:Department of Pediatrics, Faculty of Medicine, Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, 10330, Thailand.
[Ti] Título:Bufavirus in fecal specimens of patients with and without diarrhea in Thailand.
[So] Source:Arch Virol;160(7):1781-4, 2015 Jul.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bufavirus (BuV) was initially discovered in fecal samples from children with acute diarrhea. In this study, we determined the prevalence, distribution, and genotype(s) of BuV in Thailand. A total of 1,495 diarrheal and 741 non-diarrheal stool specimens were collected and analyzed. A portion of the NS1 gene of BuV was amplified by nested RT-PCR. Phylogenetic analysis was performed to classify the BuV strains found. We detected bufavirus (BuV) in diarrheal (4/1495; 0.27%) but not in non-diarrheal specimens (0/726). All four strains belonged to BuV genotype 1. BuV could be detected in adults and children, but its role in causing acute diarrhea remains unclear.
[Mh] Termos MeSH primário: Diarreia/virologia
Fezes/virologia
Infecções por Parvoviridae/virologia
Parvoviridae/isolamento & purificação
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Criança
Pré-Escolar
Diarreia/epidemiologia
Feminino
Genótipo
Seres Humanos
Lactente
Masculino
Meia-Idade
Parvoviridae/classificação
Parvoviridae/genética
Infecções por Parvoviridae/epidemiologia
Filogenia
Prevalência
Tailândia/epidemiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1508
[Cu] Atualização por classe:150613
[Lr] Data última revisão:
150613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150509
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-015-2441-z



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