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[PMID]:28410597
[Au] Autor:Liu P; Chen S; Wang M; Cheng A
[Ad] Endereço:Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, No. 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, China.
[Ti] Título:The role of nuclear localization signal in parvovirus life cycle.
[So] Source:Virol J;14(1):80, 2017 Apr 14.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Parvoviruses are small, non-enveloped viruses with an approximately 5.0 kb, single-stranded DNA genome. Usually, the parvovirus capsid gene contains one or more nuclear localization signals (NLSs), which are required for guiding the virus particle into the nucleus through the nuclear pore. However, several classical NLSs (cNLSs) and non-classical NLSs (ncNLSs) have been identified in non-structural genes, and the ncNLSs can also target non-structural proteins into the nucleus. In this review, we have summarized recent research findings on parvovirus NLSs. The capsid protein of the adeno-associated virus has four potential nuclear localization sequences, named basic region 1 (BR), BR2, BR3 and BR4. BR3 was identified as an NLS by fusing it with green fluorescent protein. Moreover, BR3 and BR4 are required for infectivity and virion assembly. In Protoparvovirus, the canine parvovirus has a common cNLS located in the VP1 unique region, similar to parvovirus minute virus of mice (MVM) and porcine parvovirus. Moreover, an ncNLS is found in the C-terminal region of MVM VP1/2. Parvovirus B19 also contains an ncNLS in the C-terminal region of VP1/2, which is essential for the nuclear transport of VP1/VP2. Approximately 1 or 2 cNLSs and 1 ncNLS have been reported in the non-structural protein of bocaviruses. Understanding the role of the NLS in the process of parvovirus infection and its mechanism of nuclear transport will contribute to the development of therapeutic vaccines and novel antiviral medicines.
[Mh] Termos MeSH primário: Sinais de Localização Nuclear
Parvovirinae/fisiologia
Proteínas não Estruturais Virais/metabolismo
Proteínas Estruturais Virais/metabolismo
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Parvovirinae/genética
Transporte Proteico
Proteínas não Estruturais Virais/genética
Proteínas Estruturais Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Nuclear Localization Signals); 0 (Viral Nonstructural Proteins); 0 (Viral Structural Proteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-017-0745-1


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Roehe, Paulo M
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[PMID]:28382380
[Au] Autor:Cibulski SP; Teixeira TF; Varela APM; Scheffer CM; Santos HF; Lima FES; Roehe PM
[Ad] Endereço:Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil. spcibulski@gmail.com.
[Ti] Título:Ungulate copiparvovirus 2 in healthy and postweaning multisystemic wasting syndrome-affected pigs.
[So] Source:Trop Anim Health Prod;49(5):945-949, 2017 Jun.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A SYBR Green-based real-time polymerase chain reaction (qPCR) was designed to detect Ungulate copiparvovirus 2, also known as porcine parvovirus 4 (PPV4). The test was applied to search for PPV4 DNAemia in sera from 1- to 4-month-old pigs displaying signs of postweaning multisystemic wasting syndrome (PMWS), as well as in sera from healthy swine at equivalent age and in sera from older healthy animals (>6 months old). High levels of PPV4 DNA were detected in PMWS-affected pigs. The mean viral DNA load in PMWS-affected pigs was 5.2 × 10 copies/mL, whereas in young healthy pigs it was 1.4 × 10 copies/mL (P ≤ 0.001). Although the copy numbers were lower in younger PMWS-affected individuals, this result sheds some light on the possible association between PPV4 viral load detection in this group and the immune impairment caused by PMWS.
[Mh] Termos MeSH primário: Infecções por Parvoviridae/veterinária
Parvovirinae/isolamento & purificação
Síndrome Definhante Multissistêmico de Suínos Desmamados/virologia
Doenças dos Suínos/epidemiologia
Carga Viral/veterinária
[Mh] Termos MeSH secundário: Animais
DNA Viral/análise
Infecções por Parvoviridae/epidemiologia
Infecções por Parvoviridae/virologia
Parvovirus Suíno/fisiologia
Prevalência
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Suínos
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-017-1279-7


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[PMID]:28284244
[Au] Autor:Souza WM; Romeiro MF; Fumagalli MJ; Modha S; de Araujo J; Queiroz LH; Durigon EL; Figueiredo LT; Murcia PR; Gifford RJ
[Ad] Endereço:1​MRC-University of Glasgow Centre for Virus Research, Glasgow, UK 2​Virology Research Center, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
[Ti] Título:Chapparvoviruses occur in at least three vertebrate classes and have a broad biogeographic distribution.
[So] Source:J Gen Virol;98(2):225-229, 2017 Feb.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chapparvoviruses are a highly divergent group of parvoviruses (family Parvoviridae) that have recently been identified via metagenomic sampling of animal faeces. Here, we report the sequences of six novel chapparvoviruses identified through both metagenomic sampling of bat tissues and in silico screening of published vertebrate genome assemblies. The novel chapparvoviruses share several distinctive genomic features and group together as a robustly supported monophyletic clade in phylogenetic trees. Our data indicate that chapparvoviruses have a broad host range in vertebrates and a global distribution.
[Mh] Termos MeSH primário: Parvovirinae/classificação
Parvovirinae/genética
Vertebrados/genética
Vertebrados/virologia
[Mh] Termos MeSH secundário: Animais
Canários/genética
Canários/virologia
Cebus/genética
Cebus/virologia
Quirópteros/genética
Quirópteros/virologia
Simulação por Computador
Evolução Molecular
Ordem dos Genes
Genoma Viral
Metagenômica
Filogenia
Filogeografia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000671


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[PMID]:27591277
[Au] Autor:Sharafeldin TA; Singh A; Abdel-Glil MY; Mor SK; Porter RE; Goyal SM
[Ad] Endereço:Department of Veterinary Population Medicine and Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, St. Paul 55108 shara022@umn.edu.
[Ti] Título:Prevalence of parvovirus in Minnesota turkeys.
[So] Source:Poult Sci;96(2):320-324, 2017 Feb 01.
[Is] ISSN:1525-3171
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Poult enteritis syndrome (PES) is characterized by enteritis and decreased body weight gain in growing turkey poults between one d and 7 wk of age. Another syndrome called light turkey syndrome (LTS) causes a decrease in body weight of adult tom turkeys in Minnesota leading to huge economic losses. Reovirus, rotavirus, and astrovirus have been found in LTS and PES flocks in Minnesota. We tested 80 fecal pools collected from four LTS flocks and 35 fecal pools from non-LTS flocks for the presence of parvovirus. In addition, 116 fecal and meconium samples from turkeys submitted to the Minnesota Veterinary Diagnostic Laboratory (MVDL) also were tested. The samples were tested by PCR using primers for the non-structural 1 (NS1) gene of parvovirus. Of the 80 samples from LTS flocks, 41 were positive for parvovirus while 20 of 35 samples from non-LTS flocks were positive. The prevalence of parvovirus in fecal samples submitted to MVDL was relatively low; only five of the 116 pools were positive. The partial NS1 gene sequences from LTS and non-LTS samples showed 98 to 100% nt identity except for one divergent turkey parvovirus (TuPV) strain that revealed 90% identity and clustered with chicken-like parvoviruses. The presence of this divergent strain suggests circulation of a recombinant strain of TuPV in Minnesota turkeys. Our results indicate that TuPVs are circulating in both LTS and non-LTS flocks of turkeys in Minnesota, and further experimental studies are indicated to study the role of TuPV in LTS.
[Mh] Termos MeSH primário: Infecções por Parvoviridae/veterinária
Parvovirinae/isolamento & purificação
Doenças das Aves Domésticas/epidemiologia
Perus
[Mh] Termos MeSH secundário: Animais
Fezes/virologia
Minnesota/epidemiologia
Infecções por Parvoviridae/epidemiologia
Infecções por Parvoviridae/virologia
Parvovirinae/genética
Filogenia
Reação em Cadeia da Polimerase/veterinária
Doenças das Aves Domésticas/virologia
Prevalência
Análise de Sequência de DNA
Proteínas não Estruturais Virais/genética
Proteínas não Estruturais Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NS1 protein, parvovirus); 0 (Viral Nonstructural Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170524
[Lr] Data última revisão:
170524
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160904
[St] Status:MEDLINE
[do] DOI:10.3382/ps/pew283


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[PMID]:27473770
[Au] Autor:Nims RW; Zhou SS
[Ad] Endereço:RMC Pharmaceutical Solutions, Inc., 1851 Lefthand Circle, Suite A, Longmont, CO 80501, USA.
[Ti] Título:Intra-family differences in efficacy of inactivation of small, non-enveloped viruses.
[So] Source:Biologicals;44(5):456-62, 2016 Sep.
[Is] ISSN:1095-8320
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The use of specific model viruses for validating viral purification process steps and for assessing the efficacies of viral disinfectants is based, in part, on the assumption that viral susceptibilities to such treatments will be similar for different members, including different genera, within a given viral family. This assumption is useful in cases where cell-based infectivity assays or laboratory strains for the specific viruses of interest might not exist. There are some documented cases, however, where exceptions to this assumption exist. In this paper, we discuss some of the more striking cases of intra-family differences in susceptibilities to inactivation steps used for downstream viral purification steps in biologics manufacture (e.g. heat inactivation, low pH, and guanidinium hydrochloride inactivation) and to specific viral disinfectants (e.g. alcohols, hydrogen peroxide, and quaternary ammonium-containing disinfectants) that might be employed for facility/equipment disinfection. The results suggest that care should be taken when extrapolating viral inactivation susceptibilities from specific model viruses to different genera or even to different members of the same genus. This should be taken into consideration by regulatory agencies and biologics manufacturers designing viral clearance and facility disinfection validation studies, and developers and evaluators of viral disinfectants.
[Mh] Termos MeSH primário: Caliciviridae/química
Desinfecção/métodos
Parvovirinae/química
Picornaviridae/química
Inativação de Vírus
[Mh] Termos MeSH secundário: Seres Humanos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170123
[Lr] Data última revisão:
170123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160731
[St] Status:MEDLINE


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[PMID]:27058516
[Au] Autor:Liu L; Schwarz L; Ullman K; Ahola H; Qiu Y; Ma Z; Hennig-Pauka I
[Ad] Endereço:1​Department of Microbiology (MIK), National Veterinary Institute (SVA), 751 89 Uppsala, Sweden.
[Ti] Título:Identification of a novel bufavirus in domestic pigs by a viral metagenomic approach.
[So] Source:J Gen Virol;97(7):1592-6, 2016 Jul.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bufavirus is a single-stranded DNA virus belonging to the genus Protoparvovirus. This study reports the identification and characterization of a porcine bufavirus by a metagenomic approach, and a limited epidemiology investigation of bufavirus in six swine farms. A comparative genome analysis showed a similarity of 93 % to a Hungarian porcine bufavirus. Bayesian and maximum-likelihood analyses of genome sequences showed a close relationship of porcine bufaviruses to human and monkey bufaviruses. Molecular dating of the most recent common ancestors supported a recent introduction of bufaviruses into human and pig populations, respectively. A real-time PCR method was developed to screen 60 faecal samples for the porcine bufavirus DNA, and eight positive samples were found in two neighbouring farms, suggesting a relatively low prevalence (13.3 %). No direct transmission of porcine bufaviruses between two neighbouring farms was found, suggesting that bufaviruses may have spread widely in different geographical regions.
[Mh] Termos MeSH primário: Genoma Viral/genética
Infecções por Parvoviridae/virologia
Parvovirinae/classificação
Parvovirinae/genética
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
DNA de Cadeia Simples/genética
DNA Viral/genética
Metagenômica/métodos
Parvovirinae/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real/métodos
Análise de Sequência de DNA
Sus scrofa
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Single-Stranded); 0 (DNA, Viral)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170519
[Lr] Data última revisão:
170519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160409
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000476


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Texto completo SciELO Brasil
[PMID]:26991274
[Au] Autor:Souza CK; Streck AF; Gonçalves KR; Pinto LD; Ravazzolo AP; de Barcellos DE; Canal CW
[Ad] Endereço:Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Brazil.
[Ti] Título:Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil.
[So] Source:Braz J Microbiol;47(2):513-7, 2016 Apr-Jun.
[Is] ISSN:1678-4405
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Ungulate tetraparvovirus 2 (UTV2), formerly known as porcine hokovirus due to its discovery in Hong Kong, is closely related to a Primate tetraparvovirus (human PARV-4) and Ungulate tetraparvovirus 1 (bovine hokovirus). Until now, UTV2 was detected in European, Asian and North American countries, but its occurrence in Latin America is still unknown. This study describes the first report of UTV2 in Brazil, as well as its phylogenetic characterization. Tissue samples (lymph node, lung, liver, spleen and kidney) of 240 piglets from eight different herds (30 animals each herd) were processed for DNA extraction. UTV2 DNA was detected by PCR and the entire VP1/VP2 gene was sequenced for phylogenetic analysis. All pigs from this study displayed postweaning multisystemic wasting syndrome (PMWS). UTV2 was detected in 55.3% of the samples distributed in the variety of porcine tissues investigated, as well as detected in almost all herds, with one exception. The phylogenetic analysis demonstrated that Brazilian UTV2 sequences were more closely related to sequences from Europe and United States.
[Mh] Termos MeSH primário: Infecções por Parvoviridae/veterinária
Parvovirinae/classificação
Parvovirinae/isolamento & purificação
Filogenia
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Brasil
DNA Viral/genética
Infecções por Parvoviridae/virologia
Parvovirinae/genética
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160319
[St] Status:MEDLINE


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[PMID]:26908891
[Au] Autor:Nuñez LF; Sá LR; Parra SH; Astolfi-Ferreira CS; Carranza C; Ferreira AJ
[Ad] Endereço:Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Orlando Marques de Paiva, 87, 05508-900, São Paulo, Brazil.
[Ti] Título:Molecular detection of chicken parvovirus in broilers with enteric disorders presenting curving of duodenal loop, pancreatic atrophy, and mesenteritis.
[So] Source:Poult Sci;95(4):802-10, 2016 Apr.
[Is] ISSN:0032-5791
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Enteric disorders are an important cause of economic losses in broiler chickens worldwide. Several agents have been associated with enteric problems, such as viruses, bacteria, and parasites. In this study, broiler chickens showing signs of enteric disorders were subjected to molecular diagnosis for several viral agents and also for pathological examination for elucidating this problem. Thus, the chickens were screened for avian nephritis virus (ANV), chicken astrovirus (CAstV), avian rotavirus (ArtV), avian reovirus (AReoV), infectious bronchitis virus (IBV), fowl adenovirus group I (FAdV-1), and chicken parvovirus (ChPV). Postmortem examinations revealed a curving of the duodenal loop (J-like appearance) and intestines filled with liquid and gaseous content. Histopathological analysis of the duodenal loop showed pancreatic atrophy, acute mesenteritis, and enteritis. PCR results showed that ChPV was the sole viral agent detected in samples with lesions such as the curved duodenal loop and pancreatic atrophy. Molecular characterization of the nucleotide and deduced amino acid sequences revealed a high similarity with other strains of ChPV from Brazil, Canada, United States, Europe, and Asia. These findings suggest an association between ChPV and the development of enteritis, pancreatitis, and pancreatic atrophy, which may lead to curling of the duodenal loop. Together, these alterations may disrupt the normal functioning of the digestive system, diminishing digestion and the absorption of dietary nutrients and consequently leading to reduced weight gain, flock impairment, dwarfism, and an elevated feed conversion rate.
[Mh] Termos MeSH primário: Galinhas
Duodeno/patologia
Síndromes de Malabsorção/veterinária
Pâncreas/patologia
Infecções por Parvoviridae/veterinária
Parvovirinae/fisiologia
Doenças das Aves Domésticas/patologia
[Mh] Termos MeSH secundário: Animais
Atrofia/patologia
Atrofia/veterinária
Síndromes de Malabsorção/patologia
Síndromes de Malabsorção/virologia
Infecções por Parvoviridae/patologia
Infecções por Parvoviridae/virologia
Parvovirinae/genética
Doenças das Aves Domésticas/virologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1606
[Cu] Atualização por classe:160329
[Lr] Data última revisão:
160329
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE
[do] DOI:10.3382/ps/pev439


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[PMID]:26890433
[Au] Autor:Chen H; Tang Y; Dou Y; Zheng X; Diao Y
[Ad] Endereço:College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, China.
[Ti] Título:Evidence for Vertical Transmission of Novel Duck-Origin Goose Parvovirus-Related Parvovirus.
[So] Source:Transbound Emerg Dis;63(3):243-7, 2016 Jun.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In 2015, novel duck-origin goose parvovirus-related parvovirus (N-GPV) infection progressively appeared in commercial Cherry Valley duck flocks in North China. Diseased ducks were observed to have beak atrophy and dwarfism syndrome (BADS). A previous study showed that a high seropositive rate for N-GPV indicated a latent infection in most breeder duck flocks. To investigate this possibility in hatching eggs collected from N-GPV-infected breeder ducks, 120 eggs were collected at various stages of embryonic development for viral DNA detection and an N-GPV-specific antibody test. N-GPV DNA was present in nine hatching eggs, eleven duck embryo and eight newly hatched ducklings. Of the newly hatched ducklings, 58.33% (21/36) were seropositive. Further, two isolates were obtained from a 12-day-old duck embryo and a newly hatched duckling. N-GPV infection did not reduce the fertilization rate and hatchability. These results indicate possible vertical transmission of N-GPV and suggest that it may be transmitted from breeder ducks to ducklings in ovo.
[Mh] Termos MeSH primário: Patos
Transmissão Vertical de Doença Infecciosa/veterinária
Infecções por Parvoviridae/veterinária
Parvovirinae/fisiologia
Doenças das Aves Domésticas/transmissão
[Mh] Termos MeSH secundário: Animais
Proteínas do Capsídeo/genética
China/epidemiologia
Óvulo/virologia
Infecções por Parvoviridae/epidemiologia
Infecções por Parvoviridae/transmissão
Infecções por Parvoviridae/virologia
Parvovirinae/genética
Filogenia
Doenças das Aves Domésticas/epidemiologia
Doenças das Aves Domésticas/virologia
Prevalência
Análise de Sequência de DNA/veterinária
Estudos Soroepidemiológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160219
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12487


  10 / 40 MEDLINE  
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[PMID]:26658622
[Au] Autor:Leuchs B; Roscher M; Müller M; Kürschner K; Rommelaere J
[Ad] Endereço:German Cancer Research Center (DKFZ), Tumor Virology F010, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany. Electronic address: B.Leuchs@dkfz.de.
[Ti] Título:Standardized large-scale H-1PV production process with efficient quality and quantity monitoring.
[So] Source:J Virol Methods;229:48-59, 2016 Mar.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The promising anticancer properties of rodent protoparvoviruses, notably H-1PV, have led to their clinical testing. This makes it necessary to produce highly pure, well-characterized virus batches in sufficient quantity. The present work focused on developing standardized production, purification, and characterization procedures as a basis for exploiting H-1PV both preclinically and in clinical trials for anticancer virotherapy. Two infection and two virus purification strategies were tested and the resulting virus preparations compared for their purity and full-, infectious-, and empty-particle contents. The adopted production process, which involves culturing and infecting NB-324K cells in 10-layer CellSTACK(®) chambers (1×10(3) infectious units per infected cell), is simple, scalable, and reproducible. Downstream processing to eliminate contaminating DNA and protein includes DNAse treatment, filtration, and two Iodixanol density-gradient centrifugations, the first gradient being a step gradient and the second, either a step (1×10(10) PFU/ml) or a continuous gradient (3×10(11) PFU/ml). A procedure was also developed for obtaining infectious particle-free preparations of empty virions for research purposes: cesium chloride density gradient centrifugation followed by UV irradiation (1×10(14) physical particles/ml). For quick, sensitive determination of physical particles (and hence, particle-to-infectivity ratios), a "Capsid-ELISA" was developed, based on a novel monoclonal antibody that specifically targets assembled capsids.
[Mh] Termos MeSH primário: Centrifugação com Gradiente de Concentração/métodos
Filtração/métodos
Parvovirinae/crescimento & desenvolvimento
Parvovirinae/isolamento & purificação
Cultura de Vírus/métodos
[Mh] Termos MeSH secundário: Linhagem Celular
Centrifugação com Gradiente de Concentração/normas
Desinfecção/métodos
Células Epiteliais/virologia
Filtração/normas
Seres Humanos
Carga Viral/métodos
Cultura de Vírus/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE



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