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[PMID]:29256289
[Au] Autor:Keros T; Jemersic L; Toplak I; Prpic J
[Ad] Endereço:1 Department of Virology, Croatian Veterinary Institute , Savska cesta 143, HR-10 000 Zagreb , Croatia.
[Ti] Título:The silent spread of Porcine Bocavirus in Croatian pigs: should we be concerned?
[So] Source:Acta Vet Hung;65(4):565-573, 2017 12.
[Is] ISSN:0236-6290
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:A survey was conducted to evaluate the presence and prevalence of Porcine Bocavirus (PBoV) in Croatian domestic pigs by means of PCR targeting the NS1 gene fragment of PBoV. This study included testing of faecal samples collected from 10 small commercial farms and 11 small backyard holdings in Croatia. The presence of PBoV was confirmed by PCR in 24 out of 57 composite faecal samples from small commercial farms and in 12 out of 43 composite faecal samples from small backyard holdings. The PCR products of 18 positive samples were sequenced for genotyping. PBoV sequences grouped into the PBoV-a, PBoV-b and PBoV-c groups with 90.81% to 99.25% nucleotide identity. All Croatian PBoV sequences showed a high nucleotide and amino acid identity with PBoV sequences from China and Hong Kong, the United States, Sweden, and Slovenia. These results clearly show that PBoV is circulating among the domestic pig population in Croatia.
[Mh] Termos MeSH primário: Bocavirus/isolamento & purificação
Infecções por Parvoviridae/veterinária
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Croácia
Infecções por Parvoviridae/epidemiologia
Infecções por Parvoviridae/virologia
Filogenia
Suínos
Doenças dos Suínos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.055


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[PMID]:28934425
[Au] Autor:Schlaberg R; Ampofo K; Tardif KD; Stockmann C; Simmon KE; Hymas W; Flygare S; Kennedy B; Blaschke A; Eilbeck K; Yandell M; McCullers JA; Williams DJ; Edwards K; Arnold SR; Bramley A; Jain S; Pavia AT
[Ad] Endereço:Department of Pathology.
[Ti] Título:Human Bocavirus Capsid Messenger RNA Detection in Children With Pneumonia.
[So] Source:J Infect Dis;216(6):688-696, 2017 Sep 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: The role of human bocavirus (HBoV) in respiratory illness is uncertain. HBoV genomic DNA is frequently detected in both ill and healthy children. We hypothesized that spliced viral capsid messenger RNA (mRNA) produced during active replication might be a better marker for acute infection. Methods: As part of the Etiology of Pneumonia in the Community (EPIC) study, children aged <18 years who were hospitalized with community-acquired pneumonia (CAP) and children asymptomatic at the time of elective outpatient surgery (controls) were enrolled. Nasopharyngeal/oropharyngeal specimens were tested for HBoV mRNA and genomic DNA by quantitative polymerase chain reaction. Results: HBoV DNA was detected in 10.4% of 1295 patients with CAP and 7.5% of 721 controls (odds ratio [OR], 1.4 [95% confidence interval {CI}, 1.0-2.0]); HBoV mRNA was detected in 2.1% and 0.4%, respectively (OR, 5.1 [95% CI, 1.6-26]). When adjusted for age, enrollment month, and detection of other respiratory viruses, HBoV mRNA detection (adjusted OR, 7.6 [95% CI, 1.5-38.4]) but not DNA (adjusted OR, 1.2 [95% CI, .6-2.4]) was associated with CAP. Among children with no other pathogens detected, HBoV mRNA (OR, 9.6 [95% CI, 1.9-82]) was strongly associated with CAP. Conclusions: Detection of HBoV mRNA but not DNA was associated with CAP, supporting a pathogenic role for HBoV in CAP. HBoV mRNA could be a useful target for diagnostic testing.
[Mh] Termos MeSH primário: Bocavirus/isolamento & purificação
Proteínas do Capsídeo/genética
Infecções por Parvoviridae/diagnóstico
Pneumonia Viral/diagnóstico
RNA Mensageiro/isolamento & purificação
RNA Viral/isolamento & purificação
[Mh] Termos MeSH secundário: Doença Aguda
Bocavirus/genética
Estudos de Casos e Controles
Criança
Pré-Escolar
Infecções Comunitárias Adquiridas/diagnóstico
Infecções Comunitárias Adquiridas/virologia
Hospitalização
Seres Humanos
Lactente
Masculino
Nasofaringe/virologia
Orofaringe/virologia
Estudos Prospectivos
Manejo de Espécimes
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (RNA, Messenger); 0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix352


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[PMID]:28613145
[Au] Autor:Woo PCY; Lau SKP; Tsoi HW; Patteril NG; Yeung HC; Joseph S; Wong EYM; Muhammed R; Chow FWN; Wernery U; Yuen KY
[Ad] Endereço:3​Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong SAR 1​State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong SAR 2​Department of Microbiology, The University of Hong Kong, Hong Kong SAR 5​Collaborative Innovation Cen
[Ti] Título:Two novel dromedary camel bocaparvoviruses from dromedaries in the Middle East with unique genomic features.
[So] Source:J Gen Virol;98(6):1349-1359, 2017 Jun.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The recent emergence of Middle East respiratory syndrome (MERS) coronavirus and its discovery from dromedary camels has boosted interest in the search for novel viruses in dromedaries. While bocaparvoviruses are known to infect various animals, it was not known that they exist in dromedaries. In this study, we describe the discovery of two novel dromedary camel bocaparvoviruses (DBoVs), DBoV1 and DBoV2, from dromedary faecal samples in Dubai. Among 667 adult dromedaries and 72 dromedary calves, 13.9 % of adult dromedaries and 33.3 % of dromedary calves were positive for DBoV1, while 7.0 % of adult dromedaries and 25.0 % of dromedary calves were positive for DBoV2, as determined by PCR. Sequencing of 21 DBoV1 and 18 DBoV2 genomes and phylogenetic analysis showed that DBoV1 and DBoV2 formed two distinct clusters, with only 32.6-36.3 % amino acid identities between the DBoV1 and DBoV2 strains. Quasispecies were detected in both DBoVs. The amino acid sequences of the NS1 proteins of all the DBoV1 and DBoV2 strains showed <85 % identity to those of all the other bocaparvoviruses, indicating that DBoV1 and DBoV2 are two bocaparvovirus species according to the ICTV criteria. Although the typical genome structure of NS1-NP1-VP1/VP2 was observed in DBoV1 and DBoV2, no phospholipase A2 motif and associated calcium binding site were observed in the predicted VP1 sequences for any of the 18 sequenced DBoV2, and no start codons were found for their VP1. For all 18 DBoV2 genomes, an AT-rich region of variable length and composition was present downstream to NP1. Further studies will be crucial to understand the pathogenic potential of DBoVs in this unique group of animals.
[Mh] Termos MeSH primário: Bocavirus/classificação
Bocavirus/isolamento & purificação
Camelus/virologia
Fezes/virologia
Infecções por Parvoviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Bocavirus/genética
Análise por Conglomerados
Ordem dos Genes
Genoma Viral
Infecções por Parvoviridae/virologia
Filogenia
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Emirados Árabes Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000775


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[PMID]:28356522
[Au] Autor:Fasina OO; Stupps S; Figueroa-Cuilan W; Pintel DJ
[Ad] Endereço:Department of Molecular Microbiology and Immunology, University of Missouri-Columbia, School of Medicine, Bond Life Sciences Center, Columbia, Missouri, USA.
[Ti] Título:Minute Virus of Canines NP1 Protein Governs the Expression of a Subset of Essential Nonstructural Proteins via Its Role in RNA Processing.
[So] Source:J Virol;91(12), 2017 Jun 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Parvoviruses use a variety of means to control the expression of their compact genomes. The bocaparvovirus minute virus of canines (MVC) encodes a small, genus-specific protein, NP1, which governs access to the viral capsid gene via its role in alternative polyadenylation and alternative splicing of the single MVC pre-mRNA. In addition to NP1, MVC encodes five additional nonstructural proteins (NS) that share an initiation codon at the left end of the genome and which are individually encoded by alternative multiply spliced mRNAs. We found that three of these proteins were encoded by mRNAs that excise the NP1-regulated MVC intron immediately upstream of the internal polyadenylation site, (pA)p, and that generation of these proteins was thus regulated by NP1. Splicing of their progenitor mRNAs joined the amino termini of these proteins to the NP1 open reading frame, and splice site mutations that prevented their expression inhibited virus replication in a host cell-dependent manner. Thus, in addition to controlling capsid gene access, NP1 also controls the expression of three of the five identified NS proteins via its role in governing MVC pre-mRNA splicing. The are small nonenveloped icosahedral viruses that are important pathogens in many animal species, including humans. Minute virus of canine (MVC) is an autonomous parvovirus in the genus It has a single promoter that generates a single pre-mRNA. NP1, a small genus-specific MVC protein, participates in the processing of this pre-mRNA and so controls capsid gene access via its role in alternative internal polyadenylation and splicing. We show that NP1 also controls the expression of three of the five identified NS proteins via its role in governing MVC pre-mRNA splicing. These NS proteins together are required for virus replication in a host cell-dependent manner.
[Mh] Termos MeSH primário: Bocavirus/fisiologia
Regulação Viral da Expressão Gênica
Processamento de RNA
RNA Viral/genética
Proteínas não Estruturais Virais/genética
Proteínas não Estruturais Virais/fisiologia
[Mh] Termos MeSH secundário: Processamento Alternativo
Animais
Bocavirus/química
Bocavirus/genética
Capsídeo/metabolismo
Proteínas do Capsídeo/genética
Códon de Iniciação
Cães
Células HEK293
Seres Humanos
Íntrons
Células Madin Darby de Rim Canino
Poliadenilação
Precursores de RNA/genética
RNA Viral/metabolismo
Transcrição Genética
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Codon, Initiator); 0 (RNA Precursors); 0 (RNA, Viral); 0 (Viral Nonstructural Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE


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[PMID]:28331084
[Au] Autor:Mietzsch M; Kailasan S; Garrison J; Ilyas M; Chipman P; Kantola K; Janssen ME; Spear J; Sousa D; McKenna R; Brown K; Söderlund-Venermo M; Baker T; Agbandje-McKenna M
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Center for Structural Biology, McKnight Brain Institute, College of Medicine, University of Florida, Gainesville, Florida, USA.
[Ti] Título:Structural Insights into Human Bocaparvoviruses.
[So] Source:J Virol;91(11), 2017 Jun 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bocaparvoviruses are emerging pathogens of the family. Human bocavirus 1 (HBoV1) causes severe respiratory infections and HBoV2 to HBoV4 cause gastrointestinal infections in young children. Recent reports of life-threatening cases, lack of direct treatment or vaccination, and a limited understanding of their disease mechanisms highlight the need to study these pathogens on a molecular and structural level for the development of therapeutics. Toward this end, the capsid structures of HBoV1, HBoV3, and HBoV4 were determined to a resolution of 2.8 to 3.0 Å by cryo-electron microscopy and three-dimensional image reconstruction. The bocaparvovirus capsids, which display different tissue tropisms, have features in common with other parvoviruses, such as depressions at the icosahedral 2-fold symmetry axis and surrounding the 5-fold symmetry axis, protrusions surrounding the 3-fold symmetry axis, and a channel at the 5-fold symmetry axis. However, unlike other parvoviruses, densities extending the 5-fold channel into the capsid interior are conserved among the bocaparvoviruses and are suggestive of a genus-specific function. Additionally, their major viral protein 3 contains loops with variable regions at their apexes conferring capsid surface topologies different from those of other parvoviruses. Structural comparisons at the strain (HBoV) and genus (bovine parvovirus and HBoV) levels identified differences in surface loops that are functionally important in host/tissue tropism, pathogenicity, and antigenicity in other parvoviruses and likely play similar roles in these viruses. This study thus provides a structural framework to characterize determinants of host/tissue tropism, pathogenicity, and antigenicity for the development of antiviral strategies to control human bocavirus infections. Human bocaviruses are one of only a few members of the family pathogenic to humans, especially young children and immunocompromised adults. There are currently no treatments or vaccines for these viruses or the related enteric bocaviruses. This study obtained the first high-resolution structures of three human bocaparvoviruses determined by cryo-reconstruction. HBoV1 infects the respiratory tract, and HBoV3 and HBoV4 infect the gastrointestinal tract, tissues that are likely targeted by the capsid. Comparison of these viruses provides information on conserved bocaparvovirus-specific features and variable regions resulting in unique surface topologies that can serve as guides to characterize HBoV determinants of tissue tropism and antigenicity in future experiments. Based on the comparison to other existing parvovirus capsid structures, this study suggests capsid regions that likely control successful infection, including determinants of receptor attachment, host cell trafficking, and antigenic reactivity. Overall, these observations could impact efforts to design antiviral strategies and vaccines for HBoVs.
[Mh] Termos MeSH primário: Capsídeo/química
Capsídeo/ultraestrutura
Bocavirus Humano/química
Bocavirus Humano/ultraestrutura
[Mh] Termos MeSH secundário: Bocavirus/química
Proteínas do Capsídeo/análise
Microscopia Crioeletrônica
Seres Humanos
Imagem Tridimensional
Proteínas Virais
Tropismo Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Viral Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171112
[Lr] Data última revisão:
171112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE


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[PMID]:28115263
[Au] Autor:Moradi P; Keyvani H; Javad Mousavi SA; Karbalaie Niya MH; Esghaei M; Bokharaei-Salim F; Ataei-Pirkooh A; Monavari SH
[Ad] Endereço:Department of Virology, Iran University of Medical Sciences, Tehran, Iran.
[Ti] Título:Investigation of viral infection in idiopathic pulmonary fibrosis among Iranian patients in Tehran.
[So] Source:Microb Pathog;104:171-174, 2017 Mar.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM OF THE STUDY: Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease, which can be lethal with chronic complications. Viral infections may be associated with IPF and other fibrotic lung diseases. In the present study, we investigate for the first time in Iran the related viral etiology of IPF in order to detect three respiratory viruses; human adenovirus, enterovirus and bocavirus. MATERIALS AND METHODS: In this cross-sectional study which was supported by Iran University of Medical Sciences, Tehran, Iran. The diagnostic criteria for IPF were based on internationally accepted clinical and imaging criteria in accordance with the 2011 IPF guidelines. 30 nasopharyngeal (NP) swabs or broncho-alveolar lavage (BAL) samples were obtained from the lung of IPF patients that were diagnosed by a sophisticated practitioner from April 2015 to February 2016. Real-time (RT) polymerase chain reaction (PCR) method was performed to detect the three viruses. Fluorescence dye of a labeled probe recorded the results in order to create positive and negative controls. SPSS version 20 software was used to calculate basic descriptive and frequency features. RESULTS: Of 30 specimens, 13 (43.4%) were male and 17 (56.6%) were female with the total mean age ± standard deviation 68.2 ± 12.0. RT-PCR assay results illustrated there was no infection of human adenovirus, enterovirus, and bocavirus detected in these samples. Significant results between IPF incidence and variables were not significant (p > 0.05). CONCLUSION: The causes of IPF in Iranian patients need more research although, based on the results of this study, there was no association between human adenovirus, enterovirus, bocavirus, and IPF.
[Mh] Termos MeSH primário: Adenovírus Humanos/isolamento & purificação
Bocavirus/isolamento & purificação
Enterovirus/isolamento & purificação
Fibrose Pulmonar Idiopática/virologia
Viroses/diagnóstico
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Líquido da Lavagem Broncoalveolar/virologia
Estudos Transversais
Feminino
Seres Humanos
Fibrose Pulmonar Idiopática/etiologia
Irã (Geográfico)/epidemiologia
Masculino
Meia-Idade
Nasofaringe/virologia
Reação em Cadeia da Polimerase em Tempo Real
Viroses/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170413
[Lr] Data última revisão:
170413
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE


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[PMID]:28100412
[Au] Autor:Sadeghi M; Kapusinszky B; Yugo DM; Phan TG; Deng X; Kanevsky I; Opriessnig T; Woolums AR; Hurley DJ; Meng XJ; Delwart E
[Ad] Endereço:Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA; Department of Virology, University of Helsinki, Finland.
[Ti] Título:Virome of US bovine calf serum.
[So] Source:Biologicals;46:64-67, 2017 Mar.
[Is] ISSN:1095-8320
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.
[Mh] Termos MeSH primário: Bovinos/sangue
Bovinos/virologia
Genoma Viral/genética
Metagenômica/métodos
[Mh] Termos MeSH secundário: Animais
Bocavirus/classificação
Bocavirus/genética
Proteínas do Capsídeo/classificação
Proteínas do Capsídeo/genética
Geografia
Infecções por Parvoviridae/veterinária
Infecções por Parvoviridae/virologia
Filogenia
Análise de Sequência de DNA
Soro/virologia
Especificidade da Espécie
Estados Unidos
Proteínas não Estruturais Virais/classificação
Proteínas não Estruturais Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Viral Nonstructural Proteins)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE


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[PMID]:27871815
[Au] Autor:Lau SK; Yeung HC; Li KS; Lam CS; Cai JP; Yuen MC; Wang M; Zheng BJ; Woo PC; Yuen KY
[Ad] Endereço:State Key Laboratory of Emerging Infectious Diseases, Hong Kong, China; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong, China; Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China; Department of Microbiology, The University of Hong Kong, H
[Ti] Título:Identification and genomic characterization of a novel rat bocavirus from brown rats in China.
[So] Source:Infect Genet Evol;47:68-76, 2017 Jan.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Despite recent discoveries of novel animal bocaparvoviruses, current understandings on the diversity and evolution of bocaparvoviruses are still limited. We report the identification and genome characterization of a novel bocaparvovirus, rat bocaparvovirus (RBoV), in brown rats (Rattus norvegicus) in China. RBoV was detected in 11.5%, 2.4%, 16.2% and 0.3% of alimentary, respiratory, spleen and kidney samples respectively, of 636 brown rats by PCR, but not in samples of other rodent species, suggesting that brown rats are the primary reservoir of RBoV. Six RBoV genomes sequenced from three brown rats revealed the presence of three ORFs, characteristic of bocaparvoviruses. Phylogenetic analysis showed that RBoV was distantly related to other bocaparvoviruses, forming a distinct cluster within the genus, with ≤55.5% nucleotide identities to the genome of ungulate bocaparvovirus 3, supporting its classification as a novel bocaparvovirus species. RBoV possessed a putative second exon encoding the C-terminal region of NS1 and conserved RNA splicing signals, similar to human bocaparvoviruses and canine bocaparvovirus. In contrast to human, feline and canine bocaparvoviruses which demonstrates inter/intra-host viral diversity, partial VP1/VP2 sequences of 49 RBoV strains demonstrated little inter-host genetic diversity, suggesting a single genetic group. Although the pathogenicity of RBoV remains to be determined, its presence in different host tissues suggests wide tissue tropism. RBoV represents the first bocaparvovirus in rodents with genome sequenced, which extends our knowledge on the host range of bocaparvoviruses. Further studies are required to better understand the epidemiology, genetic diversity and pathogenicity of bocaparvoviruses in different rodent populations.
[Mh] Termos MeSH primário: Bocavirus/genética
Genoma Viral/genética
Infecções por Parvoviridae/veterinária
Infecções por Parvoviridae/virologia
Ratos/virologia
[Mh] Termos MeSH secundário: Animais
China
DNA Viral/genética
Sítios de Splice de RNA/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (RNA Splice Sites)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE


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[PMID]:27907010
[Au] Autor:Blomström AL; Fossum C; Wallgren P; Berg M
[Ad] Endereço:Department of Biomedical Sciences and Veterinary Public Health, Section of Virology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
[Ti] Título:Viral Metagenomic Analysis Displays the Co-Infection Situation in Healthy and PMWS Affected Pigs.
[So] Source:PLoS One;11(12):e0166863, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The development of high-throughput sequencing technologies have allowed the possibility to investigate and characterise the entire microbiome of individuals, providing better insight to the complex interaction between different microorganisms. This will help to understand how the microbiome influence the susceptibility of secondary agents and development of disease. We have applied viral metagenomics to investigate the virome of lymph nodes from Swedish pigs suffering from the multifactorial disease postweaning multisystemic wasting syndrome (PMWS) as well as from healthy pigs. The aim is to increase knowledge of potential viruses, apart from porcine circovirus type 2 (PCV2), involved in PMWS development as well as to increase knowledge on the virome of healthy individuals. In healthy individuals, a diverse viral flora was seen with several different viruses present simultaneously. The majority of the identified viruses were small linear and circular DNA viruses, such as different circoviruses, anelloviruses and bocaviruses. In the pigs suffering from PMWS, PCV2 sequences were, as expected, detected to a high extent but other viruses were also identified in the background of PCV2. Apart from DNA viruses also RNA viruses were identified, among them were a porcine pestivirus showing high similarity to a recently (in 2015) discovered atypical porcine pestivirus in the US. Majority of the viruses identified in the background of PCV2 in PMWS pigs could also be identified in the healthy pigs. PCV2 sequences were also identified in the healthy pigs but to a much lower extent than in PMWS affected pigs. Although the method used here is not quantitative the very clear difference in amount of PCV2 sequences in PMWS affected pigs and healthy pigs most likely reflect the very strong replication of PCV2 known to be a hallmark of PMWS. Taken together, these findings illustrate that pigs appear to have a considerable viral flora consisting to a large extent of small single-stranded and circular DNA viruses. Future research on these types of viruses will help to better understand the role that these ubiquitous viruses may have on health and disease of pigs. We also demonstrate for the first time, in Europe, the presence of a novel porcine pestivirus.
[Mh] Termos MeSH primário: Anelloviridae/genética
Bocavirus/genética
Circovirus/genética
Pestivirus/genética
Filogenia
Síndrome Definhante Multissistêmico de Suínos Desmamados/epidemiologia
Doenças dos Suínos/epidemiologia
[Mh] Termos MeSH secundário: Anelloviridae/classificação
Anelloviridae/isolamento & purificação
Animais
Bocavirus/classificação
Bocavirus/isolamento & purificação
Circovirus/classificação
Circovirus/isolamento & purificação
Coinfecção
DNA Viral/genética
Metagenômica
Pestivirus/classificação
Pestivirus/isolamento & purificação
Síndrome Definhante Multissistêmico de Suínos Desmamados/virologia
RNA Viral/genética
Suécia/epidemiologia
Suínos
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (RNA, Viral)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166863


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[PMID]:27902362
[Au] Autor:Lau SK; Ahmed SS; Yeung HC; Li KS; Fan RY; Cheng TY; Cai JP; Wang M; Zheng BJ; Wong SS; Woo PC; Yuen KY
[Ad] Endereço:4​Department of Microbiology, The University of Hong Kong, Hong Kong SAR, PR China 3​Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong SAR, PR China 1​State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong SAR, PR China 2​Research Cen
[Ti] Título:Identification and interspecies transmission of a novel bocaparvovirus among different bat species in China.
[So] Source:J Gen Virol;97(12):3345-3358, 2016 Dec.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We report the discovery of a novel bocaparvovirus, bat bocaparvovirus (BtBoV), in one spleen, four respiratory and 61 alimentary samples from bats of six different species belonging to three families, Hipposideridae, Rhinolophidae and Vespertilionidae. BtBoV showed a higher detection rate in alimentary samples of Rhinolophus sinicus (5.7 %) than those of other bat species (0.43-1.59 %), supporting R. sinicus as the primary reservoir and virus spillover to accidental bat species. BtBoV peaked during the lactating season of R. sinicus, and it was more frequently detected among female than male adult bats (P<0.05), and among lactating than non-lactating female bats (P<0.0001). Positive BtBoV detection was associated with lower body weight in lactating bats (P<0.05). Ten nearly complete BtBoV genomes from three bat species revealed a unique large ORF1 spanning NS1 and NP1 in eight genomes and conserved splicing signals leading to multiple proteins, as well as a unique substitution in the conserved replication initiator motif within NS1. BtBoV was phylogenetically distantly related to known bocaparvoviruses with ≤57.3 % genome identities, supporting BtBoV as a novel species. Ms-BtBoV from Miniopterus schreibersii and Hp-BtBoV from Hipposideros pomona demonstrated 97.2-99.9 % genome identities with Rs-BtBoVs from R. sinicus, supporting infection of different bat species by a single BtBoV species. Rs-BtBoV_str15 represents the first bat parvovirus genome with non-coding regions sequenced, which suggested the presence of head-to-tail genomic concatamers or episomal forms of the genome. This study represents the first to describe interspecies transmission in BoVs. The high detection rates in lactating female and juvenile bats suggest possible vertical transmission of BtBoV.
[Mh] Termos MeSH primário: Bocavirus/isolamento & purificação
Quirópteros/virologia
Infecções por Parvoviridae/veterinária
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Bocavirus/classificação
Bocavirus/genética
China
Quirópteros/classificação
Feminino
Genoma Viral
Masculino
Dados de Sequência Molecular
Fases de Leitura Aberta
Infecções por Parvoviridae/transmissão
Infecções por Parvoviridae/virologia
Filogenia
Estações do Ano
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161201
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000645



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