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[PMID]:29052875
[Au] Autor:Söderlund-Venermo M
[Ad] Endereço:Department of Virology, University of Helsinki, Haartmaninkatu 3, Helsinki, 00290, Finland.
[Ti] Título:Clinical significance of parvovirus B19 DNA in cutaneous biopsies.
[So] Source:Br J Dermatol;177(4):900-901, 2017 10.
[Is] ISSN:1365-2133
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Biópsia
DNA Viral
[Mh] Termos MeSH secundário: Anticorpos Antivirais
Seres Humanos
Parvovirus
Reação em Cadeia da Polimerase
Pele
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (DNA, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171021
[St] Status:MEDLINE
[do] DOI:10.1111/bjd.15811


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[PMID]:28884224
[Au] Autor:Wang J; Huang Y; Ling J; Wang Z; Zhu G
[Ad] Endereço:College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China. wangjy@yzu.edu.cn.
[Ti] Título:Transfection of embryonated Muscovy duck eggs with a recombinant plasmid is suitable for rescue of infectious Muscovy duck parvovirus.
[So] Source:Arch Virol;162(12):3869-3874, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:For members of the family Parvoviridae, rescue of infectious virus from recombinant plasmid is usually done in cultured cells. In this study, the whole genome of the pathogenic Muscovy duck parvovirus (MDPV) strain YY was cloned into the pBluescript II (SK) vector, generating recombinant plasmid pYY. With the aid of a transfection reagent, pYY plasmid was inoculated into 11-day-old embryonated Muscovy duck eggs via the chorioallantoic membrane route, resulting in the successful rescue of infectious virus and death of the embryos. The rescued virus exhibited pathogenicity in Muscovy ducklings similar to that of its parental strain, as evaluated based on the mortality rate. The results demonstrate that plasmid transfection in embryonated Muscovy duck eggs is a convenient and efficacious method for rescue of infectious MDPV in comparison to transfection of primary cells, which is somewhat time-consuming and laborious.
[Mh] Termos MeSH primário: Patos/virologia
Parvovirus/crescimento & desenvolvimento
Parvovirus/genética
Plasmídeos
Transfecção
[Mh] Termos MeSH secundário: Animais
Clonagem Molecular
Patos/crescimento & desenvolvimento
Embrião não Mamífero/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3541-8


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[PMID]:28768875
[Au] Autor:Grosse S; Penaud-Budloo M; Herrmann AK; Börner K; Fakhiri J; Laketa V; Krämer C; Wiedtke E; Gunkel M; Ménard L; Ayuso E; Grimm D
[Ad] Endereço:Department of Infectious Diseases/Virology, Heidelberg University Hospital, Cluster of Excellence CellNetworks, Heidelberg, Germany.
[Ti] Título:Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells.
[So] Source:J Virol;91(20), 2017 Oct 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The discovery that adeno-associated virus 2 (AAV2) encodes an eighth protein, called assembly-activating protein (AAP), transformed our understanding of wild-type AAV biology. Concurrently, it raised questions about the role of AAP during production of recombinant vectors based on natural or molecularly engineered AAV capsids. Here, we show that AAP is indeed essential for generation of functional recombinant AAV2 vectors in both mammalian and insect cell-based vector production systems. Surprisingly, we observed that AAV2 capsid proteins VP1 to -3 are unstable in the absence of AAP2, likely due to rapid proteasomal degradation. Inhibition of the proteasome led to an increase of intracellular VP1 to -3 but neither triggered assembly of functional capsids nor promoted nuclear localization of the capsid proteins. Together, this underscores the crucial and unique role of AAP in the AAV life cycle, where it rapidly chaperones capsid assembly, thus preventing degradation of free capsid proteins. An expanded analysis comprising nine alternative AAV serotypes (1, 3 to 9, and rh10) showed that vector production always depends on the presence of AAP, with the exceptions of AAV4 and AAV5, which exhibited AAP-independent, albeit low-level, particle assembly. Interestingly, AAPs from all 10 serotypes could cross-complement AAP-depleted helper plasmids during vector production, despite there being distinct intracellular AAP localization patterns. These were most pronounced for AAP4 and AAP5, congruent with their inability to rescue an AAV2/AAP2 knockout. We conclude that AAP is key for assembly of genuine capsids from at least 10 different AAV serotypes, which has implications for vectors derived from wild-type or synthetic AAV capsids. Assembly of adeno-associated virus 2 (AAV2) is regulated by the assembly-activating protein (AAP), whose open reading frame overlaps with that of the viral capsid proteins. As the majority of evidence was obtained using virus-like particles composed solely of the major capsid protein VP3, AAP's role in and relevance for assembly of genuine AAV capsids have remained largely unclear. Thus, we established a -complementation assay permitting assessment of AAP functionality during production of recombinant vectors based on complete AAV capsids and derived from any serotype. We find that AAP is indeed a critical factor not only for AAV2, but also for generation of vectors derived from nine other AAV serotypes. Moreover, we identify a new role of AAP in maintaining capsid protein stability in mammalian and insect cells. Thereby, our study expands our current understanding of AAV/AAP biology, and it concomitantly provides insights into the importance of AAP for AAV vector production.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/metabolismo
Dependovirus/genética
Vetores Genéticos
Montagem de Vírus
[Mh] Termos MeSH secundário: Animais
Proteínas do Capsídeo/genética
Dependovirus/efeitos dos fármacos
Dependovirus/metabolismo
Células HeLa
Seres Humanos
Insetos
Mamíferos
Parvovirus/genética
Parvovirus/metabolismo
Complexo de Endopeptidases do Proteassoma/metabolismo
Inibidores de Proteassoma/farmacologia
Estabilidade Proteica
Células Sf9
Vírion/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Proteasome Inhibitors); EC 3.4.25.1 (Proteasome Endopeptidase Complex)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE


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[PMID]:28569640
[Au] Autor:Mahon JL; Rozanski EA; Paul AL
[Ti] Título:Prevalence of serum antibody titers against canine distemper virus and canine parvovirus in dogs hospitalized in an intensive care unit.
[So] Source:J Am Vet Med Assoc;250(12):1413-1418, 2017 Jun 15.
[Is] ISSN:1943-569X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE To determine the prevalence of dogs hospitalized in an intensive care unit (ICU) with serum antibody titers against canine distemper virus (CDV) and canine parvovirus (CPV). DESIGN Prospective observational study. ANIMALS 80 dogs. PROCEDURES Dogs hospitalized in an ICU for > 12 hours between February 1 and June 1, 2015, that had at least 0.25 mL of serum left over from diagnostic testing were eligible for study inclusion. Dogs with serum antibody titers > 1:32 (as determined by serum neutralization) and > 1:80 (as determined by hemagglutination inhibition) were considered seropositive for CDV and CPV, respectively. The date of last vaccination was obtained from the medical record of each dog. RESULTS Of the 80 dogs, 40 (50%) and 65 (81%) dogs were seropositive for CDV and CPV, respectively. Of the 40 dogs that were seronegative for CDV, 27 had been vaccinated against CDV within 3 years prior to testing. Of the 15 dogs that were seronegative for CPV, 3 had been vaccinated against CPV within 3 years prior to testing. Ten dogs were seronegative for both CDV and CPV. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated the prevalence of dogs hospitalized in an ICU that were seropositive for CDV and CPV was lower than expected given the high vaccination rate reported for dogs. Although the antibody titer necessary to prevent disease caused by CDV or CPV in critically ill dogs is unknown, adherence to infectious disease control guidelines is warranted when CDV- or CPV-infected dogs are treated in an ICU.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Vírus da Cinomose Canina/imunologia
Cinomose/prevenção & controle
Infecções por Parvoviridae/veterinária
Parvovirus/imunologia
[Mh] Termos MeSH secundário: Animais
Cinomose/sangue
Cinomose/epidemiologia
Cinomose/virologia
Cães
Feminino
Unidades de Terapia Intensiva/estatística & dados numéricos
Masculino
Infecções por Parvoviridae/epidemiologia
Infecções por Parvoviridae/prevenção & controle
Prevalência
Estudos Prospectivos
Texas/epidemiologia
Vacinação/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE
[do] DOI:10.2460/javma.250.12.1413


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[PMID]:28566084
[Au] Autor:Altay Koçak A; Öcal M; Polat M; Kanik Yüksek S; Aktas Tapisiz A; Tezer H; Özkul A; Ergünay K; Bozdayi G; Ahmed K
[Ti] Título:[Multicenter investigation of bufavirus in the etiology of viral central nervous system infections of adults and children].
[Ti] Título:Bufavirusun çocuklar ve eriskinlerdeki viral santral sinir sistemi enfeksiyonlarinin etiyolojisi açisindan çok Mmerkezli olarak arastirilmasi..
[So] Source:Mikrobiyol Bul;51(2):191-194, 2017 Apr.
[Is] ISSN:0374-9096
[Cp] País de publicação:Turkey
[La] Idioma:tur
[Ab] Resumo:Bufavirus (BuV) is a newly-identified parvovirus in the family of Parvoviridae. Metagenomic analysis of fecal samples from children in Burkina Faso with acute diarrhea showed a highly divergent parvovirus, which was named bufavirus (BuV). The global distribution, epidemiology and genetic characteristics of BuVs infections are obscure. It was first discovered as an agent causing gastroenteritis but the association of BuV infections with various clinical presentations mostly remain to be explored. The aims of this study were to investigate probable impact of BuV in central nervous system infections in a region where it was previously reported to cause human infections and to detect enteroviruses (EV) which are reported as a cause of central nervous system infections in our country. The study was undertaken in three institutions in Ankara province, Central Anatolia, Turkey. Patients, clinically diagnosed with febrile disease and/or central nervous system infections of presumed viral etiology, were enrolled in the study with informed consent. Cerebrospinal fluid specimens were collected from 93 children attended to Gazi University Hospital and Diskapi Yildirim Beyazit Hospital from October 2011-April 2015 and 33 adult patients, attended to Hacettepe University Hospital from June 2012 to March 2013. Clinical history and follow-up, physical examination and standard laboratory findings of the patients were recorded. Nucleic acid extraction was performed via commercially available spin-column assays and complementery DNA (cDNA) synthesis was performed by using commercially available cDNA synthesis kit with randomised hexamer primers. BuV detection was carried out by in house nested-polymerase chain reaction (PCR) utilized with previously-described primers. EV detection was carried out by in house PCR with pan-enterovirus primers. Seventy-four percent (93/126) and 26% (33/126) of the patients were children (0-18) and adults (19-86), respectively. In all patients, bacterial, mycobacterial and fungal cultures were negative, as well as PCR for herpes simplex virus (HSV) types 1 and 2. PCR results of all samples were negative for BuV and EV. This is the first study that evaluates a probable association of BuV and central nervous system infections. Although Parvovirus B19, a well-characterized human pathogen can rarely cause encephalitis, our findings did not confirm such an association for BuV in this preliminary investigation. However, long-term evaluation of individual cases with unknown etiology is required to reveal the relationship of the virus with specific environments.
[Mh] Termos MeSH primário: Viroses do Sistema Nervoso Central/virologia
Infecções por Parvoviridae/virologia
Parvovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Viroses do Sistema Nervoso Central/epidemiologia
Líquido Cefalorraquidiano/virologia
Criança
Pré-Escolar
Seres Humanos
Lactente
Meia-Idade
Infecções por Parvoviridae/epidemiologia
Parvovirus/classificação
Turquia/epidemiologia
Adulto Jovem
[Pt] Tipo de publicação:LETTER; MULTICENTER STUDY
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE


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[PMID]:28410608
[Au] Autor:Domanska-Blicharz K; Bocian L; Lisowska A; Jacukowicz A; Pikula A; Minta Z
[Ad] Endereço:Department of Poultry Diseases, National Veterinary Research Institute, Al. Partyzantów 57, 24-100, Pulawy, Poland. domanska@piwet.pulawy.pl.
[Ti] Título:Cross-sectional survey of selected enteric viruses in Polish turkey flocks between 2008 and 2011.
[So] Source:BMC Vet Res;13(1):108, 2017 Apr 14.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Enteric diseases are an important health problem for the intensive poultry industry, resulting in considerable economic losses. Apart from such microbiological agents associated with enteritis as bacteria and parasites, a lot of research has been recently conducted on viral origin of enteric diseases. However, enteric viruses have been identified in intestinal tract of not only diseased but also healthy poultry, so their role in enteritis is still unclear. The present study aimed at determination of the prevalence of four enteric viruses, namely astrovirus, coronavirus, parvovirus and rotavirus in meat-type turkey flocks in Poland as well as at statistical evaluation of the occurrence of the studied viruses and their relationships with the health status and the age of birds. Two hundred and seven flocks of birds aged 1-20 weeks originating from different regions of the country were investigated between 2008 and 2011. Clinical samples (10 individual faecal swabs/flock) were duly processed and examined using molecular methods targeting the conservative regions of viral genomes: RNA-dependent RNA polymerase gene of astrovirus, non-structural 1 gene of parvovirus, non-structural protein 4 gene of rotavirus, and 5' untranslated region fragment of turkey coronavirus. Different statistical methods (i.e. the independence chi-square test, the correspondence analysis and the logistic regression model) were used to establish any relationships between the analyzed data. RESULTS: Overall, 137 (66.2%, 95% CI: 59.3-72.6) of the 207 turkey flocks sampled were infected with one or more enteric viruses. Among the 137 flocks, 74 (54%, 95% CI: 45.3-62.6) were positive for one virus, whereas 54 (39.4%, 9 5% CI: 31.2-48.1) and 9 (6.6%, 95% CI: 3.1-12.1) were co-infected with two or three different enteric viruses, respectively. No flock was simultaneously infected with all four viruses studied. The prevalence of astrovirus infection was 44.9% (95% CI: 38.0-52.0), parvovirus 27.5% (95% CI: 21.6-34.2), rotavirus 18.8% (95% CI: 13.8-24.8), and coronavirus 9.7% (95% CI: 6.0-14.5). Young turkeys aged 1-4 weeks old had the highest (82.1%, 95% CI:71.7-89.8) prevalence of viral infection. Applied statistical methods have indicated the dependence of rotavirus infection as well as the co-infection with multiple viruses and the health status of turkeys. Furthermore, our results statistically confirm that especially young birds are susceptible to infection with rotavirus and astrovirus. CONCLUSIONS: The study demonstrated the presence of astrovirus, coronavirus, parvovirus and rotavirus infections in Polish turkey farms. These viruses were detected in both healthy and diseased birds. However, the presented results provide valuable feedback which could help to evaluate the role of some enteric viruses in the etiology of enteritis in turkey.
[Mh] Termos MeSH primário: Fezes/virologia
Doenças das Aves Domésticas/virologia
Perus/virologia
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Avastrovirus/genética
Avastrovirus/isolamento & purificação
Coinfecção/veterinária
Coinfecção/virologia
Coronavirus/genética
Coronavirus/isolamento & purificação
Estudos Transversais
Enterite/veterinária
Enterite/virologia
Parvovirus/genética
Parvovirus/isolamento & purificação
Polônia/epidemiologia
Rotavirus/genética
Rotavirus/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-017-1013-8


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[PMID]:28407327
[Au] Autor:Rahal A; Musher B
[Ad] Endereço:Division of Hematology-Oncology, Department of Medicine, Baylor College of Medicine, Houston, Texas.
[Ti] Título:Oncolytic viral therapy for pancreatic cancer.
[So] Source:J Surg Oncol;116(1):94-103, 2017 Jul.
[Is] ISSN:1096-9098
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Outcomes of pancreatic adenocarcinoma (PDA) remain dismal despite extensive clinical investigation. Combination chemotherapy provides modest improvements in survival above best supportive care, and immunotherapy has thus far not proven effective. Nevertheless, growing insight into antitumor immunity and the tumor microenvironment has inspired the discovery of novel agents targeting PDA. Oncolytic viruses represent an emerging class of immunotherapeutic agents that have undergone extensive preclinical investigation and warrant further investigation in well-designed clinical trials.
[Mh] Termos MeSH primário: Terapia Viral Oncolítica
Neoplasias Pancreáticas/terapia
[Mh] Termos MeSH secundário: Adenocarcinoma/imunologia
Adenocarcinoma/terapia
Adenoviridae
Animais
Ensaios Clínicos como Assunto
Seres Humanos
Vírus do Sarampo
Myxoma virus
Orthoreovirus de Mamíferos
Neoplasias Pancreáticas/imunologia
Parvovirus
Simplexvirus
Microambiente Tumoral
Vírus Vaccinia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1002/jso.24626


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[PMID]:28301545
[Au] Autor:Needell JC; Dinarello CA; Ir D; Robertson CE; Ryan SM; Kroehl ME; Frank DN; Zipris D
[Ad] Endereço:Barbara Davis Center for Childhood Diabetes, University of Colorado Denver, Aurora, Colorado, United States of America.
[Ti] Título:Implication of the intestinal microbiome as a potential surrogate marker of immune responsiveness to experimental therapies in autoimmune diabetes.
[So] Source:PLoS One;12(3):e0173968, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Type 1 diabetes (T1D) is an autoimmune proinflammatory disease with no effective intervention. A major obstacle in developing new immunotherapies for T1D is the lack of means for monitoring immune responsiveness to experimental therapies. The LEW1.WR1 rat develops autoimmunity following infection with the parvovirus Kilham rat virus (KRV) via mechanisms linked with activation of proinflammatory pathways and alterations in the gut bacterial composition. We used this animal to test the hypothesis that intervention with agents that block innate immunity and diabetes is associated with a shift in the gut microbiota. We observed that infection with KRV results in the induction of proinflammatory gene activation in both the spleen and pancreatic lymph nodes. Furthermore, administering animals the histone deacetylase inhibitor ITF-2357 and IL-1 receptor antagonist (Anakinra) induced differential STAT-1 and the p40 unit of IL-12/IL-23 gene expression. Sequencing of bacterial 16S rRNA genes demonstrated that both ITF-2357 and Anakinra alter microbial diversity. ITF-2357 and Anakinra modulated the abundance of 23 and 8 bacterial taxa in KRV-infected animals, respectively, of which 5 overlapped between the two agents. Lastly, principal component analysis implied that ITF-2357 and Anakinra induce distinct gut microbiomes compared with those from untreated animals or rats provided KRV only. Together, the data suggest that ITF-2357 and Anakinra differentially influence the innate immune system and the intestinal microbiota and highlight the potential use of the gut microbiome as a surrogate means of assessing anti-inflammatory immune effects in type 1 diabetes.
[Mh] Termos MeSH primário: Biomarcadores/metabolismo
Diabetes Mellitus Tipo 1/terapia
Intestinos/microbiologia
Microbiota
[Mh] Termos MeSH secundário: Animais
Biodiversidade
Diabetes Mellitus Tipo 1/imunologia
Diabetes Mellitus Tipo 1/microbiologia
Fezes/microbiologia
Sequenciamento de Nucleotídeos em Larga Escala
Ácidos Hidroxâmicos/farmacologia
Imunidade Inata
Proteína Antagonista do Receptor de Interleucina 1/farmacologia
Linfonodos/efeitos dos fármacos
Linfonodos/imunologia
Microbiota/efeitos dos fármacos
Pancrelipase/efeitos dos fármacos
Pancrelipase/imunologia
Parvovirus/patogenicidade
Análise de Componente Principal
RNA Ribossômico 16S/genética
Ratos
Ratos Endogâmicos Lew
Baço/efeitos dos fármacos
Baço/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Hydroxamic Acids); 0 (Interleukin 1 Receptor Antagonist Protein); 0 (RNA, Ribosomal, 16S); 53608-75-6 (Pancrelipase); Z02132R2QQ (givinostat hydrochloride)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173968


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[PMID]:28125171
[Au] Autor:Kalli IV; Adamama-Moraitou KK; Patsika MN; Pardali D; Steiner JM; Suchodolski JS; Menexes G; Brellou GD; Rallis TS
[Ad] Endereço:Companion Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
[Ti] Título:Prevalence of increased canine pancreas-specific lipase concentrations in young dogs with parvovirus enteritis.
[So] Source:Vet Clin Pathol;46(1):111-119, 2017 Mar.
[Is] ISSN:1939-165X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Pancreatic abnormalities during canine parvovirus (CPV) enteritis have not been studied prospectively. OBJECTIVES: The objective of this study was to evaluate the diagnostic significance of canine serum pancreas-specific lipase (Spec cPL) concentration in dogs with CPV enteritis for the presence of acute pancreatitis (AP). Puppies with naturally occurring CPV enteritis were recruited and prospectively allocated into 2 groups according to normal or increased serum Spec cPL concentration. Clinical signs, laboratory findings, and pancreas-associated variables were compared between groups, and the impact of possible AP on disease course, duration of hospitalization, and outcome was assessed. RESULTS: Serum Spec cPL concentration in 35 puppies was above the upper limit of the RI in 17/35 (48.6%) dogs (Group A) and within the RI in 18 dogs (Group B). An increased serum lipase activity was present in 29/35 (82.9%) dogs, and Group A dogs had a higher serum lipase activity than Group B (P = .006). Serum Spec cPL in Group A dogs was positively correlated with serum lipase activity at the day of presentation (r = .667; P = .003) and day of discharge (r = .628; P = .007). No statistically significant difference was found between groups (P = .233) for the presence of systemic inflammatory response syndrome (SIRS) (6/17 or 35.3% dogs Group A, and 8/18 or 44.4% dogs Group B), the disease course, duration of hospitalization, or outcome between groups. CONCLUSIONS: Increased serum Spec cPL is relatively common in dogs with CPV enteritis. However, such increases do not seem to correlate with the outcome of disease.
[Mh] Termos MeSH primário: Doenças do Cão/diagnóstico
Enterite/veterinária
Lipase/sangue
Pancreatite/veterinária
Infecções por Parvoviridae/veterinária
Parvovirus/isolamento & purificação
Síndrome de Resposta Inflamatória Sistêmica/veterinária
[Mh] Termos MeSH secundário: Animais
Doenças do Cão/sangue
Doenças do Cão/virologia
Cães
Enterite/sangue
Enterite/virologia
Feminino
Masculino
Pancreatite/sangue
Pancreatite/virologia
Infecções por Parvoviridae/sangue
Infecções por Parvoviridae/virologia
Prevalência
Estudos Prospectivos
Síndrome de Resposta Inflamatória Sistêmica/sangue
Síndrome de Resposta Inflamatória Sistêmica/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1111/vcp.12447


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[PMID]:28042949
[Au] Autor:Dinsart C; Pervolaraki K; Stroh-Dege A; Lavie M; Ronsse I; Rommelaere J; Van Damme J; Van Raemdonck K; Struyf S
[Ad] Endereço:1 Division of Tumor Virology, German Cancer Research Center , Heidelberg, Germany.
[Ti] Título:Recombinant Parvoviruses Armed to Deliver CXCL4L1 and CXCL10 Are Impaired in Their Antiangiogenic and Antitumoral Effects in a Kaposi Sarcoma Tumor Model Due To the Chemokines' Interference with the Virus Cycle.
[So] Source:Hum Gene Ther;28(3):295-306, 2017 Mar.
[Is] ISSN:1557-7422
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Application of oncolytic viruses is a valuable option to broaden the armament of anticancer therapies, as these combine specific cytotoxic effects and immune-stimulating properties. The self-replicating H-1 parvovirus (H-1PV) is a prototypical oncolytic virus that, besides targeting tumor cells, also infects endothelial cells, thus combining oncolytic and angiostatic traits. To increase its therapeutic value, H-1PV can be armed with cytokines or chemokines to enhance the immunological response. Some chemokines-more specifically, the CXCR3 ligands CXCL4L1 and CXCL10-combine immune-stimulating properties with angiostatic activity. This study explores the therapeutic value of recombinant parvoviruses carrying CXCL4L1 or CXCL10 transgenes (Chi-H1/CXCL4L1 or Chi-H1/CXCL10, respectively) to inhibit the growth of the human Kaposi sarcoma cell line KS-IMM. KS-IMM cells infected by Chi-H1/CXCL4L1 or Chi-H1/CXCL10 released the corresponding chemokine and showed reduced migratory capacity. Therefore, the antitumoral capacity of Chi-H1/CXCL4L1 or Chi-H1/CXCL10 was tested in mice. Either in vitro infected KS-IMM cells were injected or subcutaneously growing KS-IMM xenografts were treated by peritumoral injections of the different viruses. Surprisingly, the transgenes did not increase the antitumoral effect of natural H-1PV. Further experiments indicated that CXCL4L1 and CXCL10 interfered with the expression of the viral NS1 protein in KS-IMM cells. These results indicate that the outcome of parvovirus-based delivery of CXCR3 ligands might be tumor cell type dependent, and hence its application must be considered carefully.
[Mh] Termos MeSH primário: Quimiocina CXCL10/genética
Terapia Genética
Vetores Genéticos/administração & dosagem
Parvovirus/genética
Fator Plaquetário 4/genética
Sarcoma de Kaposi/terapia
[Mh] Termos MeSH secundário: Inibidores da Angiogênese/uso terapêutico
Animais
Antineoplásicos/uso terapêutico
Modelos Animais de Doenças
Feminino
Seres Humanos
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Nus
Sarcoma de Kaposi/irrigação sanguínea
Sarcoma de Kaposi/genética
Sarcoma de Kaposi/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiogenesis Inhibitors); 0 (Antineoplastic Agents); 0 (Chemokine CXCL10); 0 (PF4V1 protein, human); 37270-94-3 (Platelet Factor 4)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170103
[St] Status:MEDLINE
[do] DOI:10.1089/hum.2016.108



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