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[PMID]:28662150
[Au] Autor:Bovo S; Mazzoni G; Ribani A; Utzeri VJ; Bertolini F; Schiavo G; Fontanesi L
[Ad] Endereço:Department of Agricultural and Food Sciences (DISTAL), Division of Animal Sciences, University of Bologna, Bologna, Italy.
[Ti] Título:A viral metagenomic approach on a non-metagenomic experiment: Mining next generation sequencing datasets from pig DNA identified several porcine parvoviruses for a retrospective evaluation of viral infections.
[So] Source:PLoS One;12(6):e0179462, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Shot-gun next generation sequencing (NGS) on whole DNA extracted from specimens collected from mammals often produces reads that are not mapped (i.e. unmapped reads) on the host reference genome and that are usually discarded as by-products of the experiments. In this study, we mined Ion Torrent reads obtained by sequencing DNA isolated from archived blood samples collected from 100 performance tested Italian Large White pigs. Two reduced representation libraries were prepared from two DNA pools constructed each from 50 equimolar DNA samples. Bioinformatic analyses were carried out to mine unmapped reads on the reference pig genome that were obtained from the two NGS datasets. In silico analyses included read mapping and sequence assembly approaches for a viral metagenomic analysis using the NCBI Viral Genome Resource. Our approach identified sequences matching several viruses of the Parvoviridae family: porcine parvovirus 2 (PPV2), PPV4, PPV5 and PPV6 and porcine bocavirus 1-H18 isolate (PBoV1-H18). The presence of these viruses was confirmed by PCR and Sanger sequencing of individual DNA samples. PPV2, PPV4, PPV5, PPV6 and PBoV1-H18 were all identified in samples collected in 1998-2007, 1998-2000, 1997-2000, 1998-2004 and 2003, respectively. For most of these viruses (PPV4, PPV5, PPV6 and PBoV1-H18) previous studies reported their first occurrence much later (from 5 to more than 10 years) than our identification period and in different geographic areas. Our study provided a retrospective evaluation of apparently asymptomatic parvovirus infected pigs providing information that could be important to define occurrence and prevalence of different parvoviruses in South Europe. This study demonstrated the potential of mining NGS datasets non-originally derived by metagenomics experiments for viral metagenomics analyses in a livestock species.
[Mh] Termos MeSH primário: DNA Viral/genética
DNA/genética
Metagenômica
Parvovirus Suíno/genética
Viroses/genética
[Mh] Termos MeSH secundário: Animais
Estudos Retrospectivos
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 9007-49-2 (DNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179462


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[PMID]:28566374
[Au] Autor:Mészáros I; Tóth R; Olasz F; Tijssen P; Zádori Z
[Ad] Endereço:Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary meszaros.istvan@agrar.mta.hu.
[Ti] Título:The SAT Protein of Porcine Parvovirus Accelerates Viral Spreading through Induction of Irreversible Endoplasmic Reticulum Stress.
[So] Source:J Virol;91(16), 2017 Aug 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The SAT protein (SATp) of porcine parvovirus (PPV) accumulates in the endoplasmic reticulum (ER), and SAT deletion induces the slow-spreading phenotype. The comparison of the wild-type Kresse strain and its SAT knockout (SAT ) mutant revealed that prolonged cell integrity and late viral release are responsible for the slower spreading of the SAT virus. During PPV infection, regardless of the presence or absence of SATp, the expression of downstream ER stress response proteins (Xbp1 and CHOP) was induced. However, in the absence of SATp, significant differences in the quantity and the localization of CHOP were detected, suggesting a role of SATp in the induction of irreversible ER stress in infected cells. The involvement of the induction of irreversible ER stress in porcine testis (PT) cell necrosis and viral egress was confirmed by treatment of infected cells by ER stress-inducing chemicals (MG132, dithiothreitol, and thapsigargin), which accelerated the egress and spreading of both the wild-type and the SAT viruses. UV stress induction had no beneficial effect on PPV infection, underscoring the specificity of ER stress pathways in the process. However, induction of CHOP and its nuclear translocation cannot alone be responsible for the biological effect of SAT, since nuclear CHOP could not complement the lack of SAT in a coexpression experiment. SATp is encoded by an alternative open reading frame of the PPV genome. Earlier we showed that SATp of the attenuated PPV NADL-2 strain accumulates in the ER and accelerates virus release and spreading. Our present work revealed that slow spreading is a general feature of SAT PPVs and is the consequence of prolonged cell integrity. PPV infection induced ER stress in infected cells regardless of the presence of SATp, as demonstrated by the morphological changes of the ER and expression of the stress response proteins Xbp1 and CHOP. However, the presence of SATp made the ER stress more severe and accelerated cell death during infection, as shown by the higher rate of expression of CHOP and alteration of the localization of CHOP. The beneficial effect of irreversible ER stress on PPV spread was confirmed by treatment of infected cells with ER stress-inducing chemicals.
[Mh] Termos MeSH primário: Estresse do Retículo Endoplasmático
Interações Hospedeiro-Patógeno
Parvovirus Suíno/fisiologia
Proteínas Virais/metabolismo
Fatores de Virulência/metabolismo
Liberação de Vírus
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Técnicas de Inativação de Genes
Parvovirus Suíno/genética
Suínos
Proteínas Virais/genética
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins); 0 (Virulence Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE


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Roehe, Paulo M
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[PMID]:28382380
[Au] Autor:Cibulski SP; Teixeira TF; Varela APM; Scheffer CM; Santos HF; Lima FES; Roehe PM
[Ad] Endereço:Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil. spcibulski@gmail.com.
[Ti] Título:Ungulate copiparvovirus 2 in healthy and postweaning multisystemic wasting syndrome-affected pigs.
[So] Source:Trop Anim Health Prod;49(5):945-949, 2017 Jun.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A SYBR Green-based real-time polymerase chain reaction (qPCR) was designed to detect Ungulate copiparvovirus 2, also known as porcine parvovirus 4 (PPV4). The test was applied to search for PPV4 DNAemia in sera from 1- to 4-month-old pigs displaying signs of postweaning multisystemic wasting syndrome (PMWS), as well as in sera from healthy swine at equivalent age and in sera from older healthy animals (>6 months old). High levels of PPV4 DNA were detected in PMWS-affected pigs. The mean viral DNA load in PMWS-affected pigs was 5.2 × 10 copies/mL, whereas in young healthy pigs it was 1.4 × 10 copies/mL (P ≤ 0.001). Although the copy numbers were lower in younger PMWS-affected individuals, this result sheds some light on the possible association between PPV4 viral load detection in this group and the immune impairment caused by PMWS.
[Mh] Termos MeSH primário: Infecções por Parvoviridae/veterinária
Parvovirinae/isolamento & purificação
Síndrome Definhante Multissistêmico de Suínos Desmamados/virologia
Doenças dos Suínos/epidemiologia
Carga Viral/veterinária
[Mh] Termos MeSH secundário: Animais
DNA Viral/análise
Infecções por Parvoviridae/epidemiologia
Infecções por Parvoviridae/virologia
Parvovirus Suíno/fisiologia
Prevalência
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Suínos
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-017-1279-7


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[PMID]:28340426
[Au] Autor:Cao L; Chen J; Wei Y; Shi H; Zhang X; Yuan J; Shi D; Liu J; Zhu X; Wang X; Cui S; Feng L
[Ad] Endereço:Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150040, China.
[Ti] Título:Porcine parvovirus induces activation of NF-κB signaling pathways in PK-15 cells mediated by toll-like receptors.
[So] Source:Mol Immunol;85:248-255, 2017 May.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Porcine parvovirus (PPV) is a pathogenic factor that primarily induces severe reproductive failure of pregnant swine, which results in extensive losses to the swine industry worldwide. In this study, a potential mechanism of PPV-induced activation of the nuclear transcription factor-kappaB (NF-κB) by infection in porcine kidney cells (PK-15) was elucidated for the first time. The subcellular localization of p65 analyzed by immunofluorescence assay (IFA) showed that PPV infection induced p65 translocation from the cytoplasm to the nucleus. p65 phosphorylation was detected in PK-15 cells with progression of PPV infection. NF-κB-regulated gene expression was enhanced in a viral dose-dependent manner using the NF-κB luciferase reporter assay system. Furthermore, PPV-induced NF-κB activation was closely related to the inhibitory kappa B alpha (IκBα) degradation. Treatment with a NF-κB-specific inhibitor demonstrated that the production of PPV progeny viruses was enhanced to some extent. In addition, these results demonstrated that the adapter molecule TIR domain-containing adapter inducing IFN-ß (TRIF) and myeloid differentiation primary-response protein 88 (MyD88)-dependent signaling pathways were involved in PPV-induced NF-κB activation. Together, these results provide evidence that the toll-like receptor (TLR) pathway participates in recognition of PPV and induction of NF-κB activation, and add to understanding of the molecular mechanisms underlying PPV infection.
[Mh] Termos MeSH primário: NF-kappa B/imunologia
Infecções por Parvoviridae/veterinária
Parvovirus Suíno/imunologia
Transdução de Sinais/imunologia
Receptores Toll-Like/imunologia
[Mh] Termos MeSH secundário: Animais
Western Blotting
Linhagem Celular
Imunofluorescência
NF-kappa B/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Suínos
Receptores Toll-Like/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NF-kappa B); 0 (Toll-Like Receptors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE


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[PMID]:28215695
[Au] Autor:Huangfu C; Ma Y; Jia J; Lv M; Zhu F; Ma X; Zhao X; Zhang J
[Ad] Endereço:Beijing Key Laboratory of Blood Safety and Supply Technologies, Beijing Institute of Transfusion Medicine, Beijing, 100850, China.
[Ti] Título:Inactivation of viruses by pasteurization at 60 °C for 10 h with and without 40% glucose as stabilizer during a new manufacturing process of α2-Macroglobulin from Cohn Fraction IV.
[So] Source:Biologicals;46:139-142, 2017 Mar.
[Is] ISSN:1095-8320
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pasteurization is regularly used to inactivate viruses for the safety of plasma derivatives. Influence of pasteurization at 60 °C for 10 h on α2-Macroglobulin activity and virus inactivation were studied. With 40% sugar as stabilizers more than 70% α2-Macroglobulin activity was reserved after pasteurization compared with 20% in control. Glucose presented a better activity protection effect than sucrose and maltose. By pasteurization without stabilizer the virus titers of pseudorabies virus, Sindbis virus, porcine parvovirus and encephalomyocarditis virus were reduced more than 5.88 log , 7.50 log , 4.88 log , and 5.63 log respectively within 2 h. By pasteurization with 40% glucose vesicular stomatitis virus was inactivated more than 5.88 log within 1 h. Only 2.71 log reduction was achieved for encephalomyocarditis virus after 10 h. 40% glucose protected α2-M activity and viruses simultaneously from pasteurization. Other viral inactivation methods need to be incorporated to ensure viral safety of this manufacturing process of α2-Macroglobulin.
[Mh] Termos MeSH primário: Proteínas Sanguíneas/metabolismo
Glucose/farmacologia
Temperatura Alta
Pasteurização/métodos
Inativação de Vírus/efeitos dos fármacos
alfa-Macroglobulinas/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Vírus da Encefalomiocardite/fisiologia
Herpesvirus Suídeo 1/fisiologia
Seres Humanos
Parvovirus Suíno/fisiologia
Reprodutibilidade dos Testes
Vírus Sindbis/fisiologia
Suínos
Fatores de Tempo
Células Vero
Vírus da Estomatite Vesicular Indiana/efeitos dos fármacos
Vírus da Estomatite Vesicular Indiana/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Proteins); 0 (Cohn fraction IV); 0 (alpha-Macroglobulins); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170418
[Lr] Data última revisão:
170418
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


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[PMID]:27590228
[Au] Autor:Cui J; Fan J; Gerber PF; Biernacka K; Stadejek T; Xiao CT; Opriessnig T
[Ad] Endereço:The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
[Ti] Título:First identification of porcine parvovirus 6 in Poland.
[So] Source:Virus Genes;53(1):100-104, 2017 Feb.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Porcine parvovirus type 1 is a major causative agent of swine reproductive failure. During the past decade, several new parvoviruses have been discovered in pigs. Porcine parvovirus type 6 (PPV6), recently identified, has been reported in pigs in China and in the USA while the PPV6 status in the European pig population remains undetermined. In the present study, PPV6 DNA was identified in serum samples collected from domestic pigs in Poland. In investigated herds, the prevalence of PPV6 was 14.9 % (15/101 samples). Sequencing was conducted, and 11 nearly complete PPV6 genomes were obtained. Phylogenetic analysis indicated that PPV6 sequences cluster into four distinct groups, and the Polish PPV6 strains from three individual farms were present in three of these four groups. In addition, the Polish PPV6 strain P15-1 was identified as a putative recombination of an ORF1 from US stains and an ORF2 from Chinese strains. This is the first identification of PPV6 in Europe, and this finding will encourage future epidemiological studies on parvoviruses in European pigs.
[Mh] Termos MeSH primário: Infecções por Parvoviridae/veterinária
Parvovirus Suíno/genética
Doenças dos Suínos/epidemiologia
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
DNA Viral
Evolução Molecular
Genoma Viral
Fases de Leitura Aberta
Parvovirus Suíno/classificação
Filogenia
Polônia/epidemiologia
Análise de Sequência de DNA
Sus scrofa
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160904
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-016-1386-y


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[PMID]:27593155
[Au] Autor:Yang Y; Qin X; Zhang W; Li Y; Zhang Z
[Ad] Endereço:State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Xujiaping 1, Lanzhou, 730046, Gansu, China.
[Ti] Título:Rapid and specific detection of porcine parvovirus by isothermal recombinase polymerase amplification assays.
[So] Source:Mol Cell Probes;30(5):300-305, 2016 Oct.
[Is] ISSN:1096-1194
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Porcine parvovirus (PPV) is a major cause of swine reproductive failure and reported in many countries worldwide. Recombinase polymerase amplification (RPA) assays using a real-time fluorescent detection (PPV real-time RPA assay) and a lateral flow dipstick (PPV RPA LFD assay) were developed targeting PPV NS1 gene. The detection limit of PPV real-time RPA assay was 300 copies per reaction within 9 min at 38 °C, while the RPA LFD assay has a detection limit of 400 copies per reaction in less than 20 min at 38 °C. In both assays, there were no cross-reactions with porcine circovirus type 2, pseudorabies virus, porcine reproductive and respiratory syndrome virus, classical swine fever virus, and foot-and-mouth disease virus. Based on a total of 128 clinical samples examined, the sensitivity and the specificity of the developed RPA assays for identification of PPV was 94.4% and 100%, respectively, when compared to real-time (qPCR) assay. Therefore, the RPA assay provides a rapid, sensitive and specific alternative for PPV detection.
[Mh] Termos MeSH primário: Parvovirus Suíno/genética
Parvovirus Suíno/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real/métodos
Recombinases/metabolismo
Temperatura Ambiente
[Mh] Termos MeSH secundário: Animais
Sensibilidade e Especificidade
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160906
[St] Status:MEDLINE


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[PMID]:27396164
[Au] Autor:Fu P; Pan X; Han Q; Yang X; Zhu Q; Guo X; Zhang Y; Chen H
[Ti] Título:[Immune Response of Recombinant Pseudorabies Virus rPRV-VP2 Expressing VP2 Gene of Porcine Parvovirus in Mice].
[So] Source:Bing Du Xue Bao;32(2):195-202, 2016 Mar.
[Is] ISSN:1000-8721
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:In order to develop a combined live vaccine that will be used to prevent against porcine parvovirus (PPV) and Pseudorabies virus (PRV) infection, the VP2 gene of PPV was inserted into the transfer vector plasmid pG to produce the recombinant plasmid pGVP2. The plasmid pGVP2 and the genome of PRV HB98 attenuated vaccine were transfected by using lipofectamine into swine testis cells for the homologous recombination. The recombinant virus rPRV-VP2 was purified by selection of green fluorescence plaques for five cycles. 6-week-old female Kunming mice were immunized intramuscularly with attenuated PRV parent HB98 strain, commercial inactivated vaccine against PPV, recombinant virus, DMEM culture solution. The injections were repeated with an equivalent dose after 2 weeks in all of the groups, and then challenged with the virulent PRV NY strain at 7 weeks after the first immunization. The recombinant virus rPRV-VP2 was successfully generated, and the recombinant virus could effectively elicite anti-PPV and PRV antibody and significant cellular immune response as indicated by anti-PPV ELISA and HI, PRV-neutralizing assay and flow cytometry. The challenge assay indicated that recombinant virus could protect the mice against the virulent PRV challenge. These results demonstrated that the recombinant virus can be a candidate recombinant vaccine strain for the prevention of PRV and PPV.
[Mh] Termos MeSH primário: Antígenos Virais/imunologia
Proteínas do Capsídeo/imunologia
Parvovirus Suíno/imunologia
Doenças dos Suínos/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/imunologia
Antígenos Virais/administração & dosagem
Antígenos Virais/genética
Proteínas do Capsídeo/administração & dosagem
Proteínas do Capsídeo/genética
Feminino
Expressão Gênica
Vetores Genéticos/genética
Vetores Genéticos/metabolismo
Herpesvirus Suídeo 1/genética
Herpesvirus Suídeo 1/metabolismo
Camundongos
Parvovirus Suíno/genética
Suínos
Doenças dos Suínos/prevenção & controle
Doenças dos Suínos/virologia
Vacinas Virais/administração & dosagem
Vacinas Virais/genética
Vacinas Virais/imunologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (Capsid Proteins); 0 (VP2 protein, porcine parvovirus); 0 (Viral Vaccines)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161021
[Lr] Data última revisão:
161021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160712
[St] Status:MEDLINE


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[PMID]:27033924
[Au] Autor:McKillen J; McNair I; Lagan P; McKay K; McClintock J; Casement V; Charreyre C; Allan G
[Ad] Endereço:Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, Belfast BT4 3SD, United Kingdom. Electronic address: john.mckillen@afbini.gov.uk.
[Ti] Título:Reproduction of post-weaning multi-systemic wasting syndrome in an animal disease model as a tool for vaccine testing under controlled conditions.
[So] Source:Res Vet Sci;105:143-52, 2016 Apr.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Snatch farrowed, colostrum deprived piglets were inoculated with different combinations of porcine circovirus 2, porcine parvovirus and Erysipelothrix rhusiopathiae candidate vaccines. 10 piglets were mock-vaccinated. Following virus challenge with a combined porcine circovirus 2/porcine parvovirus inoculum, all animals were monitored and samples taken for serology, immunohistochemistry and qPCR. At 24 dpc all non-vaccinated animals remaining were exhibiting signs of post-weaning multi-systemic wasting syndrome which was confirmed by laboratory analysis. Details of the study, analysis of samples and performance of the candidate vaccines are described.
[Mh] Termos MeSH primário: Circovirus/imunologia
Síndrome Definhante Multissistêmico de Suínos Desmamados/imunologia
Vacinas Virais/farmacologia
[Mh] Termos MeSH secundário: Animais
Vacinas Bacterianas/farmacologia
Modelos Animais de Doenças
Erysipelothrix/imunologia
Infecções por Erysipelothrix/imunologia
Infecções por Erysipelothrix/microbiologia
Infecções por Parvoviridae/imunologia
Infecções por Parvoviridae/virologia
Parvovirus Suíno/imunologia
Síndrome Definhante Multissistêmico de Suínos Desmamados/virologia
Suínos
Doenças dos Suínos/imunologia
Doenças dos Suínos/microbiologia
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Vaccines); 0 (Viral Vaccines)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE


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[PMID]:26995221
[Au] Autor:Palinski RM; Mitra N; Hause BM
[Ad] Endereço:Kansas State Department of Diagnostic Medicine and Pathobiology, 1800 Denison Avenue, Manhattan, KS, 66506, USA.
[Ti] Título:Discovery of a novel Parvovirinae virus, porcine parvovirus 7, by metagenomic sequencing of porcine rectal swabs.
[So] Source:Virus Genes;52(4):564-7, 2016 Aug.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Parvoviruses are a diverse group of viruses containing some of the smallest known species that are capable of infecting a wide range of animals. Metagenomic sequencing of pooled rectal swabs from adult pigs identified a 4103-bp contig consisting of two major open reading frames encoding proteins of 672 and 469 amino acids (aa) in length. BLASTP analysis of the 672-aa protein found 42.4 % identity to fruit bat (Eidolon helvum) parvovirus 2 (EhPV2) and 37.9 % to turkey parvovirus (TuPV) TP1-2012/HUN NS1 proteins. The 469-aa protein had no significant similarity to known proteins. Genetic and phylogenetic analyses suggest that PPV7, EhPV2, and TuPV represent a novel genus in the family Parvoviridae. Quantitative PCR screening of 182 porcine diagnostic samples found a total of 16 positives (8.6 %). Together, these data suggest that PPV7 is a highly divergent novel parvovirus prevalent within the US swine.
[Mh] Termos MeSH primário: Genoma Viral/genética
Infecções por Parvoviridae/virologia
Parvovirus Suíno/genética
Doenças dos Suínos/virologia
Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Metagenômica/métodos
Filogenia
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160321
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-016-1322-1



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